Rroxscaffold_7G00179550

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003462
Physical Location & Seq
Forward (+)
19079110 .. 19080615
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00179550.1

Sequence Viewer

Length: 1506 bp
ATGTTTCTCGAGCTACTAGCCTTGGCCCTTTTCTTGCTCCCCTTGCTTTTCCTCATTCTCAAGTTTTCCGGCAACCAGGAAACCGGCAACAGCAAGCTCCCAAAATCATACCCAATAATCGGTTCATTCCCAGCCCTCTACAAGAACCATGCCAACAGACTCGCATGGTTCACTGAGATTCTCCAGAACTCTCCCTCGGCCACTATCTCTATTCACCACTCTTTCGGCCAACACTCCGTCATCACCGCCAACCCCACTGTGGTTCAGCACATCCTCAAGACAAACTTCCCCACCTACCAAAAAGGCGAGTCATCCCGTCAAATTCTCCAGGAGCTCCTCGGCGACGGCATTTTCAACGCCGACGGTGACAACTGGAAGTTCCAGAGGCAACTCTCCAGCCACGAATTCAACACCAAGTCGCTGAGAAAATTCGTTGAAGACGTGGTTGACATTGAACTCTACGACCGGCTCATACCCATTCTATCCAAATCTTCTAGCGAGCGAACCATTCTTGATTTCCAAGACATTCTCCAAAGGTTTGCCTTCGACAACATTTGCCGAATCGGGTTCGGCTACGACCCTGAGTATCTCCTTCCTTCTCTGCCACAAGCTAAATTCGCGGTGGCGTTTGACGATGCGGTGCAGATCAGCAGCGACAGGTTCGGCTACCTGAGACCGGTGTGGAAAATCAAAAGAGCTCTTGGAATCGGCTCGGAAAGCCGTATCAGCAGAGTGGTCTCCGATGTGCGTGAGTTCGCCAACACCATTGTCAAGCAAAAGAAGAGAGAGATGGACGAGATGATGAAGACCCAGGAGTCTCACGATCTGCTGTCTCGGTTCTTGGGCTCCGGCCACTCCGACGAGAAGTTTGTCACCGACATCGTCATCAGCTTCACCCTCGCCGGAAGAGACACCACATCAGCGGCTCTGACCTGGTTCTTCTGGCTAATCTCAAACCACCCAAACGTCGAGAGAGAAATTCTCAAGGAGATAGAAGAGAAATCGGAGAACGGAGCCGGATATGATGAAGCGAAGGAGATGGTGTACACACACGCGGCGCTCTGCGAGAGCATGAGGCTGTACCCGCCGGTTCCGATAGACAGCAAAGAGGCGGTGAACGACGACGTTTTGCCGGACGGGACTAAAGTGAAGAAGGGGATGGCAGTGTCGTACCATGTTTACGCGATGGGGAGGATGGAGCAGATATGGGGCAAGGACTGGGCAGAGTTCCGACCCGAGAGGTGGTTGGAGCGTGACGTGGCGACGAACAAGTGGGGGTTCGTGGCGAAGGACTCGTTTAGCTACCCGGTGTTTCAGGCGGGGCCGAGGATCTGTTTGGGGAAGGAGATGGCGTTTTTGCAGATGAAGAGGGTGGTGGTTGGGGTTTTGAAGAGGTTTAAGGTGGTGCCAGAGAAGAGAGAGAATGGATCAGAGCCGGAGTTTGTTGCTTACCTTACCGCGAAAATGAAGGGTGGGTTTCCGGTCACCGTTGTGGAGAGGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
p450

Protein Analysis

501

Amino Acids

57.15

Weight (kDa)

7.19

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 34 - 474 1.2e-60 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 814
AccB1I GGYRCC 1 cut(s) 1405
AccII CGCG 4 cut(s) 620, 1055, 1184, 1460
AciI CCGC 9 cut(s) 246, 620, 638, 923, 1055, 1085, 1112, 1319, 1458
AclWI GGATC 2 cut(s) 1337, 1435
AcoI YGGCCR 3 cut(s) 198, 226, 850
AcsI RAATTY 5 cut(s) 321, 404, 428, 614, 978
AfaI GTAC 3 cut(s) 1046, 1082, 1172
AfiI CCNNNNNNNGG 4 cut(s) 119, 259, 676, 1242
AgeI ACCGGT 1 cut(s) 676
AgsI TTSAA 5 cut(s) 355, 409, 437, 455, 1390
AjiI CACGTC 2 cut(s) 442, 1258
AjnI CCWGG 4 cut(s) 75, 327, 810, 932
AjuI GAANNNNNNNTTGG 2 cut(s) 106, 138
AleI CACNNNNGTG 1 cut(s) 1490
AluBI AGCT 7 cut(s) 13, 97, 334, 611, 698, 891, 1302
AluI AGCT 7 cut(s) 13, 97, 334, 611, 698, 891, 1302
Alw21I GWGCWC 2 cut(s) 336, 700
Alw26I GTCTC 5 cut(s) 667, 742, 822, 837, 903
AlwI GGATC 2 cut(s) 1337, 1435
Ama87I CYCGRG 2 cut(s) 8, 1235
AoxI GGCC 5 cut(s) 24, 198, 226, 850, 1322
ApeKI GCWGC 1 cut(s) 651
ApoI RAATTY 5 cut(s) 321, 404, 428, 614, 978
ArsI GACNNNNNNTTYG 2 cut(s) 851, 883
AsiGI ACCGGT 1 cut(s) 676
AspLEI GCGC 1 cut(s) 1060
AspS9I GGNCC 2 cut(s) 25, 1322
AsuC2I CCSGG 1 cut(s) 1307
AsuHPI GGTGA 7 cut(s) 206, 235, 377, 865, 886, 1126, 1477
AvaI CYCGRG 2 cut(s) 8, 1235
BanI GGYRCC 1 cut(s) 1405
BanII GRGCYC 3 cut(s) 336, 700, 848
BbsI GAAGAC 2 cut(s) 444, 812
Bbv12I GWGCWC 2 cut(s) 336, 700
BbvI GCAGC 1 cut(s) 663
BccI CCATC 6 cut(s) 784, 1033, 1153, 1180, 1189, 1342
BceAI ACGGC 2 cut(s) 361, 705
BciT130I CCWGG 4 cut(s) 77, 329, 812, 934
BcnI CCSGG 1 cut(s) 1307
BcoDI GTCTC 5 cut(s) 667, 742, 822, 837, 903
BfaI CTAG 2 cut(s) 17, 495
BfoI RGCGCY 1 cut(s) 1061
BisI GCNGC 3 cut(s) 652, 924, 1056
BlsI GCNGC 3 cut(s) 653, 925, 1057
Bme1390I CCNGG 5 cut(s) 77, 329, 812, 934, 1307
BmeT110I CYCGRG 2 cut(s) 8, 1235
BmgBI CACGTC 2 cut(s) 442, 1258
BmgT120I GGNCC 2 cut(s) 25, 1322
BmiI GGNNCC 5 cut(s) 847, 1015, 1092, 1323, 1407
BmrFI CCNGG 5 cut(s) 77, 329, 812, 934, 1307
BmrI ACTGGG 1 cut(s) 1228
BmsI GCATC 1 cut(s) 625
BmuI ACTGGG 1 cut(s) 1228
BpiI GAAGAC 2 cut(s) 444, 812
BplI GAGNNNNNCTC 2 cut(s) 966, 998
BpmI CTGGAG 3 cut(s) 167, 311, 379
BpuEI CTTGAG 3 cut(s) 44, 260, 968
BpuMI CCSGG 1 cut(s) 1307
BsaI GGTCTC 2 cut(s) 667, 742
BsaJI CCNNGG 5 cut(s) 21, 195, 337, 810, 1325
BsaWI WCCGGW 2 cut(s) 676, 1480
BsaXI ACNNNNNCTCC 4 cut(s) 1028, 1058, 1337, 1367
Bsc4I CCNNNNNNNGG 4 cut(s) 119, 259, 676, 1242
Bse118I RCCGGY 4 cut(s) 83, 465, 676, 1087
Bse1I ACTGG 2 cut(s) 377, 1223
BseBI CCWGG 4 cut(s) 77, 329, 812, 934
BseDI CCNNGG 5 cut(s) 21, 195, 337, 810, 1325
BseGI GGATG 4 cut(s) 270, 311, 1164, 1200
BseLI CCNNNNNNNGG 4 cut(s) 119, 259, 676, 1242
BseMII CTCAG 4 cut(s) 165, 413, 573, 662
BseNI ACTGG 2 cut(s) 377, 1223
BseRI GAGGAG 1 cut(s) 326
BseXI GCAGC 1 cut(s) 663
BseYI CCCAGC 1 cut(s) 130
BsgI GTGCAG 1 cut(s) 662
Bsh1236I CGCG 4 cut(s) 620, 1055, 1184, 1460
Bsh1285I CGRYCG 1 cut(s) 466
BshFI GGCC 5 cut(s) 26, 200, 228, 852, 1324
BshNI GGYRCC 1 cut(s) 1405
BshTI ACCGGT 1 cut(s) 676
BsiEI CGRYCG 1 cut(s) 466
BsiHKAI GWGCWC 2 cut(s) 336, 700
BsiHKCI CYCGRG 2 cut(s) 8, 1235
BslFI GGGAC 1 cut(s) 1153
BslI CCNNNNNNNGG 4 cut(s) 119, 259, 676, 1242
BsmAI GTCTC 5 cut(s) 667, 742, 822, 837, 903
BsmFI GGGAC 1 cut(s) 1153
BsnI GGCC 5 cut(s) 26, 200, 228, 852, 1324
Bso31I GGTCTC 2 cut(s) 667, 742
BsoBI CYCGRG 2 cut(s) 8, 1235
Bsp1286I GDGCHC 3 cut(s) 336, 700, 848
Bsp1407I TGTACA 1 cut(s) 1044
Bsp143I GATC 4 cut(s) 645, 823, 1329, 1427
BspACI CCGC 9 cut(s) 246, 620, 638, 923, 1055, 1085, 1112, 1319, 1458
BspANI GGCC 5 cut(s) 26, 200, 228, 852, 1324
BspCNI CTCAG 4 cut(s) 166, 414, 574, 663
BspFNI CGCG 4 cut(s) 620, 1055, 1184, 1460
BspLI GGNNCC 5 cut(s) 847, 1015, 1092, 1323, 1407
BspPI GGATC 2 cut(s) 1337, 1435
BspT107I GGYRCC 1 cut(s) 1405
BspTNI GGTCTC 2 cut(s) 667, 742
BsrFI RCCGGY 4 cut(s) 83, 465, 676, 1087
BsrGI TGTACA 1 cut(s) 1044
BsrI ACTGG 2 cut(s) 377, 1223
BssAI RCCGGY 4 cut(s) 83, 465, 676, 1087
BssECI CCNNGG 5 cut(s) 21, 195, 337, 810, 1325
BssMI GATC 4 cut(s) 645, 823, 1329, 1427
BssT1I CCWWGG 1 cut(s) 21
Bst2UI CCWGG 4 cut(s) 77, 329, 812, 934
Bst4CI ACNGT 3 cut(s) 259, 365, 1489
Bst6I CTCTTC 6 cut(s) 776, 901, 990, 1361, 1385, 1409
BstAUI TGTACA 1 cut(s) 1044
BstC8I GCNNGC 2 cut(s) 95, 500
BstDEI CTNAG 4 cut(s) 174, 422, 582, 671
BstEII GGTNACC 1 cut(s) 1483
BstF5I GGATG 4 cut(s) 270, 311, 1164, 1200
BstFNI CGCG 4 cut(s) 620, 1055, 1184, 1460
BstH2I RGCGCY 1 cut(s) 1061
BstHHI GCGC 1 cut(s) 1060
BstKTI GATC 4 cut(s) 648, 826, 1332, 1430
BstMAI GTCTC 5 cut(s) 667, 742, 822, 837, 903
BstMBI GATC 4 cut(s) 645, 823, 1329, 1427
BstMCI CGRYCG 1 cut(s) 466
BstMWI GCNNNNNNNGC 5 cut(s) 43, 617, 717, 726, 1084
BstNI CCWGG 4 cut(s) 77, 329, 812, 934
BstPI GGTNACC 1 cut(s) 1483
BstSCI CCNGG 5 cut(s) 75, 327, 810, 932, 1305
BstUI CGCG 4 cut(s) 620, 1055, 1184, 1460
BstV1I GCAGC 1 cut(s) 663
BstV2I GAAGAC 2 cut(s) 444, 812
BstX2I RGATCY 1 cut(s) 1329
BstYI RGATCY 1 cut(s) 1329
BsuRI GGCC 5 cut(s) 26, 200, 228, 852, 1324
BtgZI GCGATG 1 cut(s) 1199
BtrI CACGTC 2 cut(s) 442, 1258
BtsCI GGATG 4 cut(s) 270, 311, 1164, 1200
BtsI GCAGTG 1 cut(s) 1170
BtsIMutI CAGTG 3 cut(s) 171, 255, 1170
Cac8I GCNNGC 2 cut(s) 95, 500
CfoI GCGC 1 cut(s) 1060
Cfr10I RCCGGY 4 cut(s) 83, 465, 676, 1087
Cfr13I GGNCC 2 cut(s) 25, 1322
CsiI ACCWGGT 1 cut(s) 932
Csp6I GTAC 3 cut(s) 1045, 1081, 1171
CspAI ACCGGT 1 cut(s) 676
CviAII CATG 4 cut(s) 149, 165, 1072, 1175
CviQI GTAC 3 cut(s) 1045, 1081, 1171
DdeI CTNAG 4 cut(s) 174, 422, 582, 671
DpnI GATC 4 cut(s) 647, 825, 1331, 1429
DpnII GATC 4 cut(s) 645, 823, 1329, 1427
DrdI GACNNNNNNGTC 1 cut(s) 814
DseDI GACNNNNNNGTC 1 cut(s) 814
EaeI YGGCCR 3 cut(s) 198, 226, 850
Eam1104I CTCTTC 6 cut(s) 776, 901, 990, 1361, 1385, 1409
EarI CTCTTC 6 cut(s) 776, 901, 990, 1361, 1385, 1409
Ecl136II GAGCTC 2 cut(s) 334, 698
Eco130I CCWWGG 1 cut(s) 21
Eco24I GRGCYC 3 cut(s) 336, 700, 848
Eco31I GGTCTC 2 cut(s) 667, 742
Eco53kI GAGCTC 2 cut(s) 334, 698
Eco88I CYCGRG 2 cut(s) 8, 1235
Eco91I GGTNACC 1 cut(s) 1483
EcoICRI GAGCTC 2 cut(s) 334, 698
EcoO65I GGTNACC 1 cut(s) 1483
EcoRI GAATTC 1 cut(s) 404
EcoRII CCWGG 4 cut(s) 75, 327, 810, 932
EcoT14I CCWWGG 1 cut(s) 21
EcoT38I GRGCYC 3 cut(s) 336, 700, 848
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 4 cut(s) 152, 168, 1075, 1178
FaiI YATR 8 cut(s) 109, 150, 166, 473, 1023, 1073, 1176, 1207
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FaqI GGGAC 1 cut(s) 1153
FatI CATG 4 cut(s) 148, 164, 1071, 1174
FauI CCCGC 2 cut(s) 1092, 1312
Fnu4HI GCNGC 3 cut(s) 652, 924, 1056
FokI GGATG 4 cut(s) 257, 298, 1171, 1207
FriOI GRGCYC 3 cut(s) 336, 700, 848
Fsp4HI GCNGC 3 cut(s) 652, 924, 1056
FspBI CTAG 2 cut(s) 17, 495
GlaI GCGC 1 cut(s) 1059
GluI GCNGC 3 cut(s) 652, 924, 1056
GsaI CCCAGC 1 cut(s) 134
GsuI CTGGAG 3 cut(s) 167, 311, 379
HaeII RGCGCY 1 cut(s) 1061
HaeIII GGCC 5 cut(s) 26, 200, 228, 852, 1324
HhaI GCGC 1 cut(s) 1060
Hin1II CATG 4 cut(s) 152, 168, 1075, 1178
Hin6I GCGC 1 cut(s) 1058
HinP1I GCGC 1 cut(s) 1058
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HinfI GANTC 7 cut(s) 159, 178, 308, 561, 705, 815, 1292
HphI GGTGA 7 cut(s) 206, 235, 377, 865, 886, 1126, 1477
Hpy166II GTNNAC 6 cut(s) 171, 448, 1045, 1047, 1117, 1180
Hpy188I TCNGA 8 cut(s) 715, 742, 859, 930, 1006, 1095, 1232, 1432
Hpy188III TCNNGA 7 cut(s) 8, 184, 277, 382, 512, 821, 970
Hpy8I GTNNAC 6 cut(s) 171, 448, 1045, 1047, 1117, 1180
Hpy99I CGWCG 7 cut(s) 347, 365, 863, 971, 1124, 1127, 1267
HpyAV CCTTC 8 cut(s) 553, 602, 606, 1027, 1147, 1282, 1336, 1462
HpyCH4III ACNGT 3 cut(s) 259, 365, 1489
HpyCH4IV ACGT 4 cut(s) 441, 966, 1125, 1257
HpyCH4V TGCA 2 cut(s) 643, 1360
HpyF10VI GCNNNNNNNGC 5 cut(s) 43, 617, 717, 726, 1084
HpyF3I CTNAG 4 cut(s) 174, 422, 582, 671
HpySE526I ACGT 4 cut(s) 441, 966, 1125, 1257
Hsp92II CATG 4 cut(s) 152, 168, 1075, 1178
HspAI GCGC 1 cut(s) 1058
Kzo9I GATC 4 cut(s) 645, 823, 1329, 1427
LmnI GCTCC 8 cut(s) 42, 102, 331, 339, 851, 1013, 1198, 1249
Lsp1109I GCAGC 1 cut(s) 663
LweI GCATC 1 cut(s) 625
MabI ACCWGGT 1 cut(s) 932
MaeI CTAG 2 cut(s) 17, 495
MaeII ACGT 4 cut(s) 441, 966, 1125, 1257
MaeIII GTNAC 4 cut(s) 365, 871, 1253, 1483
MalI GATC 4 cut(s) 647, 825, 1331, 1429
MboI GATC 4 cut(s) 645, 823, 1329, 1427
MflI RGATCY 1 cut(s) 1329
MhlI GDGCHC 3 cut(s) 336, 700, 848
MluCI AATT 5 cut(s) 321, 404, 428, 614, 978
MlyI GAGTC 4 cut(s) 153, 317, 824, 1286
MmeI TCCRAC 3 cut(s) 882, 1227, 1255
MseI TTAA 2 cut(s) 1398, 1504
MslI CAYNNNNRTG 1 cut(s) 1490
MspA1I CMGCKG 1 cut(s) 923
MspR9I CCNGG 5 cut(s) 77, 329, 812, 934, 1307
MvaI CCWGG 4 cut(s) 77, 329, 812, 934
MvnI CGCG 4 cut(s) 620, 1055, 1184, 1460
MwoI GCNNNNNNNGC 5 cut(s) 43, 617, 717, 726, 1084
NciI CCSGG 1 cut(s) 1307
NdeII GATC 4 cut(s) 645, 823, 1329, 1427
NlaIII CATG 4 cut(s) 152, 168, 1075, 1178
NlaIV GGNNCC 5 cut(s) 847, 1015, 1092, 1323, 1407
NmeAIII GCCGAG 3 cut(s) 176, 318, 1350
NmuCI GTSAC 4 cut(s) 365, 871, 1253, 1483
OliI CACNNNNGTG 1 cut(s) 1490
PaeR7I CTCGAG 1 cut(s) 8
PcsI WCGNNNNNNNCGW 1 cut(s) 438
PfeI GAWTC 3 cut(s) 178, 561, 705
PflFI GACNNNGTC 1 cut(s) 881
PfoI TCCNGGA 1 cut(s) 327
PinAI ACCGGT 1 cut(s) 676
PkrI GCNGC 3 cut(s) 653, 925, 1057
PleI GAGTC 4 cut(s) 153, 316, 823, 1286
PpsI GAGTC 4 cut(s) 153, 316, 823, 1286
Psp124BI GAGCTC 2 cut(s) 336, 700
Psp6I CCWGG 4 cut(s) 75, 327, 810, 932
PspEI GGTNACC 1 cut(s) 1483
PspFI CCCAGC 1 cut(s) 130
PspGI CCWGG 4 cut(s) 75, 327, 810, 932
PspN4I GGNNCC 5 cut(s) 847, 1015, 1092, 1323, 1407
PspPI GGNCC 2 cut(s) 25, 1322
PsuI RGATCY 1 cut(s) 1329
PsyI GACNNNGTC 1 cut(s) 881
RsaI GTAC 3 cut(s) 1046, 1082, 1172
RsaNI GTAC 3 cut(s) 1045, 1081, 1171
RseI CAYNNNNRTG 1 cut(s) 1490
SacI GAGCTC 2 cut(s) 336, 700
SaqAI TTAA 2 cut(s) 1398, 1504
SatI GCNGC 3 cut(s) 652, 924, 1056
Sau3AI GATC 4 cut(s) 645, 823, 1329, 1427
Sau96I GGNCC 2 cut(s) 25, 1322
SchI GAGTC 4 cut(s) 153, 317, 824, 1286
ScrFI CCNGG 5 cut(s) 77, 329, 812, 934, 1307
SduI GDGCHC 3 cut(s) 336, 700, 848
SexAI ACCWGGT 1 cut(s) 932
SfaNI GCATC 1 cut(s) 625
Sfr274I CTCGAG 1 cut(s) 8
SlaI CTCGAG 1 cut(s) 8
SmiMI CAYNNNNRTG 1 cut(s) 1490
SmlI CTYRAG 4 cut(s) 8, 59, 275, 983
SmoI CTYRAG 4 cut(s) 8, 59, 275, 983
Sse9I AATT 5 cut(s) 321, 404, 428, 614, 978
SsiI CCGC 9 cut(s) 246, 620, 638, 923, 1055, 1085, 1112, 1319, 1458
SspMI CTAG 2 cut(s) 17, 495
SstI GAGCTC 2 cut(s) 336, 700
StyD4I CCNGG 5 cut(s) 75, 327, 810, 932, 1305
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 3 cut(s) 259, 365, 1489
TaiI ACGT 4 cut(s) 444, 969, 1128, 1260
TaqI TCGA 3 cut(s) 9, 546, 969
TasI AATT 5 cut(s) 321, 404, 428, 614, 978
TatI WGTACW 1 cut(s) 1044
TauI GCSGC 2 cut(s) 926, 1058
TfiI GAWTC 3 cut(s) 178, 561, 705
Tru1I TTAA 2 cut(s) 1398, 1504
Tru9I TTAA 2 cut(s) 1398, 1504
TscAI CASTG 3 cut(s) 178, 262, 1170
TseFI GTSAC 4 cut(s) 365, 871, 1253, 1483
TseI GCWGC 1 cut(s) 651
Tsp45I GTSAC 4 cut(s) 365, 871, 1253, 1483
TspDTI ATGAA 5 cut(s) 114, 818, 1041, 1379, 1481
TspGWI ACGGA 2 cut(s) 226, 1026
TspRI CASTG 3 cut(s) 178, 262, 1170
Tth111I GACNNNGTC 1 cut(s) 881
XapI RAATTY 5 cut(s) 321, 404, 428, 614, 978
XcmI CCANNNNNNNNNTGG 1 cut(s) 256
XhoI CTCGAG 1 cut(s) 8
XspI CTAG 2 cut(s) 17, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.