RchiOBHm_Chr6g0295261

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0018950
Physical Location & Seq
Forward (+)
57332787 .. 57333572
786 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26496

Sequence Viewer

Length: 513 bp
ATGCTGAGAGTAACGGACAAGCTATTGGAGGTGCTTTCAGAGGGTCTAGGTCTGGATAGGAAGGTATTGAAATCCCATATGGGAGATGGAGCAGTGGAATTGGAAATGAAGATAAATATGTACCCGCCATGCCCACAACCCCAACTAGCCCTTGGCGTTGAGCCTCACACCGACATGTCCGCCCTCACCTTACTGGTTTCGAACGATGTTCGTGGCCTTCAGCTGTGGAAAGATGACAATTGGGTTGCTGTGAATTACCTGCCAAATGCAGTCTTTGTTAACATTGGTGATCAGATGGAGGTCCTGAGCAATGGTAAGTACAAGAGTGTTCTTCACAGGAGCTTGGTGAACAAGGAACGGTTGCGCATTTCATGGGCTGTGTTCATTGCGCCACCACATGAGGCTGTGATTGGGCCTCTTCCGGAGCTTGTGGACGAGCAAAATCCTGCCAAATACTCAACCAAAACATATGGTGAATACCGACACCGCAAATTCAACAAGATCCCACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00941 ko01100 ko01110 map00941 map01100 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

170

Amino Acids

19.25

Weight (kDa)

6.97

Isoelectric Point (pI)

33.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 36 - 131 4.7e-32 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 365
Acc36I ACCTGC 1 cut(s) 267
AccIII TCCGGA 1 cut(s) 421
AciI CCGC 3 cut(s) 125, 180, 487
AclWI GGATC 1 cut(s) 496
AcsI RAATTY 1 cut(s) 491
AcuI CTGAAG 1 cut(s) 203
AfaI GTAC 2 cut(s) 122, 320
AflIII ACRYGT 1 cut(s) 174
AgsI TTSAA 2 cut(s) 70, 496
AluBI AGCT 4 cut(s) 22, 223, 342, 427
AluI AGCT 4 cut(s) 22, 223, 342, 427
AlwI GGATC 1 cut(s) 496
Aor13HI TCCGGA 1 cut(s) 421
AoxI GGCC 2 cut(s) 214, 413
ApoI RAATTY 1 cut(s) 491
AspLEI GCGC 2 cut(s) 366, 391
AspS9I GGNCC 2 cut(s) 301, 413
AsuHPI GGTGA 4 cut(s) 178, 299, 358, 485
AsuII TTCGAA 1 cut(s) 200
AvaII GGWCC 1 cut(s) 301
BccI CCATC 2 cut(s) 80, 289
BclI TGATCA 1 cut(s) 289
BfaI CTAG 2 cut(s) 47, 146
BfuAI ACCTGC 1 cut(s) 267
Bme18I GGWCC 1 cut(s) 301
BmgT120I GGNCC 2 cut(s) 301, 413
Bpu10I CCTNAGC 1 cut(s) 305
Bpu14I TTCGAA 1 cut(s) 200
BsaJI CCNNGG 1 cut(s) 151
BsaWI WCCGGW 1 cut(s) 421
Bse1I ACTGG 1 cut(s) 198
Bse3DI GCAATG 2 cut(s) 316, 384
BseAI TCCGGA 1 cut(s) 421
BseDI CCNNGG 1 cut(s) 151
BseMI GCAATG 2 cut(s) 316, 384
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 1 cut(s) 198
BshFI GGCC 2 cut(s) 216, 415
BsiSI CCGG 1 cut(s) 422
BsnI GGCC 2 cut(s) 216, 415
Bsp119I TTCGAA 1 cut(s) 200
Bsp13I TCCGGA 1 cut(s) 421
Bsp143I GATC 2 cut(s) 289, 501
BspACI CCGC 3 cut(s) 125, 180, 487
BspANI GGCC 2 cut(s) 216, 415
BspCNI CTCAG 1 cut(s) 297
BspEI TCCGGA 1 cut(s) 421
BspMI ACCTGC 1 cut(s) 267
BspPI GGATC 1 cut(s) 496
BspT104I TTCGAA 1 cut(s) 200
BsrDI GCAATG 2 cut(s) 316, 384
BsrI ACTGG 1 cut(s) 198
BssECI CCNNGG 1 cut(s) 151
BssMI GATC 2 cut(s) 289, 501
BssT1I CCWWGG 1 cut(s) 151
Bst4CI ACNGT 2 cut(s) 360, 510
Bst6I CTCTTC 1 cut(s) 423
BstBI TTCGAA 1 cut(s) 200
BstDEI CTNAG 2 cut(s) 5, 305
BstHHI GCGC 2 cut(s) 366, 391
BstKTI GATC 2 cut(s) 292, 504
BstMBI GATC 2 cut(s) 289, 501
BstNSI RCATGY 1 cut(s) 178
BstX2I RGATCY 1 cut(s) 501
BstYI RGATCY 1 cut(s) 501
BsuRI GGCC 2 cut(s) 216, 415
BtsI GCAGTG 1 cut(s) 99
BtsIMutI CAGTG 1 cut(s) 99
BveI ACCTGC 1 cut(s) 267
CfoI GCGC 2 cut(s) 366, 391
Cfr13I GGNCC 2 cut(s) 301, 413
Csp6I GTAC 2 cut(s) 121, 319
CviAII CATG 4 cut(s) 129, 175, 372, 398
CviQI GTAC 2 cut(s) 121, 319
DdeI CTNAG 2 cut(s) 5, 305
DpnI GATC 2 cut(s) 291, 503
DpnII GATC 2 cut(s) 289, 501
Eam1104I CTCTTC 1 cut(s) 423
EarI CTCTTC 1 cut(s) 423
EciI GGCGGA 1 cut(s) 169
Eco130I CCWWGG 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 301
Eco57I CTGAAG 1 cut(s) 203
EcoO109I RGGNCCY 1 cut(s) 301
EcoT14I CCWWGG 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 151
FaeI CATG 4 cut(s) 132, 178, 375, 401
FaiI YATR 9 cut(s) 78, 80, 119, 130, 176, 373, 399, 469, 471
FatI CATG 4 cut(s) 128, 174, 371, 397
FauI CCCGC 1 cut(s) 132
FauNDI CATATG 2 cut(s) 78, 469
FbaI TGATCA 1 cut(s) 289
FspBI CTAG 2 cut(s) 47, 146
FspI TGCGCA 1 cut(s) 365
GlaI GCGC 2 cut(s) 365, 390
HaeIII GGCC 2 cut(s) 216, 415
HapII CCGG 1 cut(s) 422
HhaI GCGC 2 cut(s) 366, 391
Hin1II CATG 4 cut(s) 132, 178, 375, 401
Hin6I GCGC 2 cut(s) 364, 389
HinP1I GCGC 2 cut(s) 364, 389
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HpaI GTTAAC 1 cut(s) 280
HpaII CCGG 1 cut(s) 422
HphI GGTGA 4 cut(s) 178, 299, 358, 485
Hpy166II GTNNAC 3 cut(s) 280, 349, 433
Hpy188I TCNGA 2 cut(s) 40, 294
Hpy188III TCNNGA 3 cut(s) 53, 304, 422
Hpy8I GTNNAC 3 cut(s) 280, 349, 433
HpyAV CCTTC 2 cut(s) 55, 227
HpyCH4III ACNGT 2 cut(s) 360, 510
HpyCH4V TGCA 1 cut(s) 269
HpyF3I CTNAG 2 cut(s) 5, 305
Hsp92II CATG 4 cut(s) 132, 178, 375, 401
HspAI GCGC 2 cut(s) 364, 389
Kpn2I TCCGGA 1 cut(s) 421
Ksp22I TGATCA 1 cut(s) 289
KspAI GTTAAC 1 cut(s) 280
Kzo9I GATC 2 cut(s) 289, 501
LmnI GCTCC 3 cut(s) 89, 339, 424
LpnPI CCDG 7 cut(s) 38, 179, 272, 317, 322, 435, 459
MaeI CTAG 2 cut(s) 47, 146
MaeIII GTNAC 1 cut(s) 10
MalI GATC 2 cut(s) 291, 503
MboI GATC 2 cut(s) 289, 501
MboII GAAGA 3 cut(s) 121, 323, 410
MfeI CAATTG 1 cut(s) 238
MflI RGATCY 1 cut(s) 501
MluCI AATT 4 cut(s) 98, 238, 253, 491
MnlI CCTC 7 cut(s) 22, 34, 174, 194, 292, 394, 426
MroI TCCGGA 1 cut(s) 421
MseI TTAA 1 cut(s) 279
MslI CAYNNNNRTG 1 cut(s) 173
MspA1I CMGCKG 1 cut(s) 223
MspI CCGG 1 cut(s) 422
MunI CAATTG 1 cut(s) 238
NdeI CATATG 2 cut(s) 78, 469
NdeII GATC 2 cut(s) 289, 501
NlaIII CATG 4 cut(s) 132, 178, 375, 401
NsbI TGCGCA 1 cut(s) 365
NspI RCATGY 1 cut(s) 178
NspV TTCGAA 1 cut(s) 200
PciI ACATGT 1 cut(s) 174
PpuMI RGGWCCY 1 cut(s) 301
PscI ACATGT 1 cut(s) 174
Psp5II RGGWCCY 1 cut(s) 301
PspPI GGNCC 2 cut(s) 301, 413
PspPPI RGGWCCY 1 cut(s) 301
PsuI RGATCY 1 cut(s) 501
PvuII CAGCTG 1 cut(s) 223
RsaI GTAC 2 cut(s) 122, 320
RsaNI GTAC 2 cut(s) 121, 319
RseI CAYNNNNRTG 1 cut(s) 173
SaqAI TTAA 1 cut(s) 279
Sau3AI GATC 2 cut(s) 289, 501
Sau96I GGNCC 2 cut(s) 301, 413
SfuI TTCGAA 1 cut(s) 200
SinI GGWCC 1 cut(s) 301
SmiMI CAYNNNNRTG 1 cut(s) 173
Sse9I AATT 4 cut(s) 98, 238, 253, 491
SsiI CCGC 3 cut(s) 125, 180, 487
SspMI CTAG 2 cut(s) 47, 146
StyI CCWWGG 1 cut(s) 151
TaaI ACNGT 2 cut(s) 360, 510
TaqI TCGA 1 cut(s) 200
TasI AATT 4 cut(s) 98, 238, 253, 491
TatI WGTACW 1 cut(s) 318
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TscAI CASTG 1 cut(s) 99
TspDTI ATGAA 3 cut(s) 122, 360, 373
TspGWI ACGGA 1 cut(s) 29
TspRI CASTG 1 cut(s) 99
VpaK11BI GGWCC 1 cut(s) 301
XapI RAATTY 1 cut(s) 491
XceI RCATGY 1 cut(s) 178
XcmI CCANNNNNNNNNTGG 2 cut(s) 83, 149
XspI CTAG 2 cut(s) 47, 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.