Rh1DG093200

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0018950
Physical Location & Seq
Forward (+)
17522721 .. 17523158
438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG093200.1

Sequence Viewer

Length: 438 bp
ATGGGAGATGGAGAAGTGGAACTGGAAATGAAGATAAATATGTACCCGCCATGCCCACAACCCCAACGAGCCCTTGGCGTTGAGCCTCACACCGACATGTCCGCCCTCACCTTACTGCAGGTTTCAAACGATGATCCTGGTCTTCAGCTGTGGAAAGATGACACTTGGGTTGCTGTGAATTACCTGCCAAATGCAGTCTTTGCTAACATTGGTGATCAGATGGAGGTCTTGAGCAATGTTAAGTTCAAGAGTATTCTTCACAGGAGCTTGGTGAACAAGAAGCGGTTACACATGTCATGGGCTGTGTTCATTGTGGCGCCACATGAAGCTGTGATTGGGCCTCTGCGGGAGCTTGTGGAAGAGCAAAATCCTGCCAAATACTCAACCAAAACATATGGTGAATACCGACACCACAAATTCAACAAGATCCCACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00941 ko01100 ko01110 map00941 map01100 map01110
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

145

Amino Acids

16.55

Weight (kDa)

6.29

Isoelectric Point (pI)

47.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 10 - 104 4.2e-25 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 109, 192
AccB1I GGYRCC 1 cut(s) 316
AciI CCGC 4 cut(s) 47, 102, 283, 346
AclWI GGATC 2 cut(s) 128, 421
AcsI RAATTY 1 cut(s) 416
AcuI CTGAAG 1 cut(s) 128
AcyI GRCGYC 1 cut(s) 317
AfaI GTAC 1 cut(s) 44
AflIII ACRYGT 2 cut(s) 96, 291
AgsI TTSAA 3 cut(s) 126, 247, 421
AjnI CCWGG 1 cut(s) 136
AjuI GAANNNNNNNTTGG 2 cut(s) 318, 350
AluBI AGCT 4 cut(s) 148, 267, 329, 352
AluI AGCT 4 cut(s) 148, 267, 329, 352
AlwI GGATC 2 cut(s) 128, 421
AoxI GGCC 1 cut(s) 338
ApoI RAATTY 1 cut(s) 416
AspLEI GCGC 1 cut(s) 319
AspS9I GGNCC 1 cut(s) 338
AsuHPI GGTGA 4 cut(s) 100, 224, 283, 410
BanI GGYRCC 1 cut(s) 316
BanII GRGCYC 1 cut(s) 73
BbsI GAAGAC 1 cut(s) 134
BccI CCATC 2 cut(s) 2, 214
BciT130I CCWGG 1 cut(s) 138
BclI TGATCA 1 cut(s) 214
BfmI CTRYAG 1 cut(s) 116
BfoI RGCGCY 1 cut(s) 320
BfuAI ACCTGC 2 cut(s) 109, 192
Bme1390I CCNGG 1 cut(s) 138
BmgT120I GGNCC 1 cut(s) 338
BmiI GGNNCC 1 cut(s) 318
BmrFI CCNGG 1 cut(s) 138
BpiI GAAGAC 1 cut(s) 134
BpuEI CTTGAG 1 cut(s) 250
BsaHI GRCGYC 1 cut(s) 317
BsaJI CCNNGG 1 cut(s) 73
Bse1I ACTGG 1 cut(s) 27
Bse3DI GCAATG 1 cut(s) 241
BseBI CCWGG 1 cut(s) 138
BseDI CCNNGG 1 cut(s) 73
BseMI GCAATG 1 cut(s) 241
BseNI ACTGG 1 cut(s) 27
BshFI GGCC 1 cut(s) 340
BshNI GGYRCC 1 cut(s) 316
BsnI GGCC 1 cut(s) 340
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 3 cut(s) 133, 214, 426
BspACI CCGC 4 cut(s) 47, 102, 283, 346
BspANI GGCC 1 cut(s) 340
BspLI GGNNCC 1 cut(s) 318
BspMAI CTGCAG 1 cut(s) 120
BspMI ACCTGC 2 cut(s) 109, 192
BspPI GGATC 2 cut(s) 128, 421
BspQI GCTCTTC 1 cut(s) 354
BspT107I GGYRCC 1 cut(s) 316
BsrDI GCAATG 1 cut(s) 241
BsrI ACTGG 1 cut(s) 27
BssECI CCNNGG 1 cut(s) 73
BssMI GATC 3 cut(s) 133, 214, 426
BssNI GRCGYC 1 cut(s) 317
BssT1I CCWWGG 1 cut(s) 73
Bst2UI CCWGG 1 cut(s) 138
Bst4CI ACNGT 1 cut(s) 435
Bst6I CTCTTC 1 cut(s) 354
BstACI GRCGYC 1 cut(s) 317
BstAPI GCANNNNNTGC 1 cut(s) 200
BstH2I RGCGCY 1 cut(s) 320
BstHHI GCGC 1 cut(s) 319
BstKTI GATC 3 cut(s) 136, 217, 429
BstMBI GATC 3 cut(s) 133, 214, 426
BstMWI GCNNNNNNNGC 1 cut(s) 200
BstNI CCWGG 1 cut(s) 138
BstNSI RCATGY 2 cut(s) 100, 295
BstSCI CCNGG 1 cut(s) 136
BstSFI CTRYAG 1 cut(s) 116
BstV2I GAAGAC 1 cut(s) 134
BstX2I RGATCY 1 cut(s) 426
BstYI RGATCY 1 cut(s) 426
BsuRI GGCC 1 cut(s) 340
BveI ACCTGC 2 cut(s) 109, 192
CfoI GCGC 1 cut(s) 319
Cfr13I GGNCC 1 cut(s) 338
Csp6I GTAC 1 cut(s) 43
CviAII CATG 5 cut(s) 51, 97, 292, 297, 323
CviJI RGCY 8 cut(s) 71, 85, 148, 267, 302, 329, 340, 352
CviKI_1 RGCY 8 cut(s) 71, 85, 148, 267, 302, 329, 340, 352
CviQI GTAC 1 cut(s) 43
DinI GGCGCC 1 cut(s) 318
DpnI GATC 3 cut(s) 135, 216, 428
DpnII GATC 3 cut(s) 133, 214, 426
Eam1104I CTCTTC 1 cut(s) 354
EarI CTCTTC 1 cut(s) 354
EciI GGCGGA 1 cut(s) 91
Eco130I CCWWGG 1 cut(s) 73
Eco24I GRGCYC 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 128
EcoRII CCWGG 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 73
EcoT38I GRGCYC 1 cut(s) 73
EgeI GGCGCC 1 cut(s) 318
EheI GGCGCC 1 cut(s) 318
ErhI CCWWGG 1 cut(s) 73
FaeI CATG 5 cut(s) 54, 100, 295, 300, 326
FaiI YATR 8 cut(s) 41, 52, 98, 293, 298, 324, 394, 396
FatI CATG 5 cut(s) 50, 96, 291, 296, 322
FauI CCCGC 2 cut(s) 54, 339
FauNDI CATATG 1 cut(s) 394
FbaI TGATCA 1 cut(s) 214
FriOI GRGCYC 1 cut(s) 73
GlaI GCGC 1 cut(s) 318
HaeII RGCGCY 1 cut(s) 320
HaeIII GGCC 1 cut(s) 340
HhaI GCGC 1 cut(s) 319
Hin1I GRCGYC 1 cut(s) 317
Hin1II CATG 5 cut(s) 54, 100, 295, 300, 326
Hin6I GCGC 1 cut(s) 317
HinP1I GCGC 1 cut(s) 317
HphI GGTGA 4 cut(s) 100, 224, 283, 410
Hpy166II GTNNAC 1 cut(s) 274
Hpy188I TCNGA 1 cut(s) 219
Hpy188III TCNNGA 2 cut(s) 229, 247
Hpy8I GTNNAC 1 cut(s) 274
HpyCH4III ACNGT 1 cut(s) 435
HpyCH4V TGCA 2 cut(s) 118, 194
HpyF10VI GCNNNNNNNGC 1 cut(s) 200
Hsp92I GRCGYC 1 cut(s) 317
Hsp92II CATG 5 cut(s) 54, 100, 295, 300, 326
HspAI GCGC 1 cut(s) 317
KasI GGCGCC 1 cut(s) 316
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 3 cut(s) 133, 214, 426
LguI GCTCTTC 1 cut(s) 354
LmnI GCTCC 2 cut(s) 264, 349
LpnPI CCDG 7 cut(s) 8, 104, 123, 150, 197, 247, 384
MaeIII GTNAC 1 cut(s) 285
MalI GATC 3 cut(s) 135, 216, 428
MboI GATC 3 cut(s) 133, 214, 426
MboII GAAGA 4 cut(s) 43, 134, 248, 371
MflI RGATCY 1 cut(s) 426
MhlI GDGCHC 1 cut(s) 73
MluCI AATT 2 cut(s) 178, 416
Mly113I GGCGCC 1 cut(s) 317
MnlI CCTC 4 cut(s) 96, 116, 217, 351
MseI TTAA 1 cut(s) 240
MslI CAYNNNNRTG 1 cut(s) 95
MspA1I CMGCKG 1 cut(s) 148
MspR9I CCNGG 1 cut(s) 138
MvaI CCWGG 1 cut(s) 138
MwoI GCNNNNNNNGC 1 cut(s) 200
NarI GGCGCC 1 cut(s) 317
NdeI CATATG 1 cut(s) 394
NdeII GATC 3 cut(s) 133, 214, 426
NlaIII CATG 5 cut(s) 54, 100, 295, 300, 326
NlaIV GGNNCC 1 cut(s) 318
NspI RCATGY 2 cut(s) 100, 295
PciI ACATGT 2 cut(s) 96, 291
PciSI GCTCTTC 1 cut(s) 354
PluTI GGCGCC 1 cut(s) 320
PscI ACATGT 2 cut(s) 96, 291
Psp6I CCWGG 1 cut(s) 136
PspGI CCWGG 1 cut(s) 136
PspN4I GGNNCC 1 cut(s) 318
PspPI GGNCC 1 cut(s) 338
PstI CTGCAG 1 cut(s) 120
PsuI RGATCY 1 cut(s) 426
PvuII CAGCTG 1 cut(s) 148
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
RseI CAYNNNNRTG 1 cut(s) 95
SapI GCTCTTC 1 cut(s) 354
SaqAI TTAA 1 cut(s) 240
Sau3AI GATC 3 cut(s) 133, 214, 426
Sau96I GGNCC 1 cut(s) 338
ScrFI CCNGG 1 cut(s) 138
SduI GDGCHC 1 cut(s) 73
SetI ASST 8 cut(s) 113, 123, 150, 186, 228, 269, 331, 354
SfcI CTRYAG 1 cut(s) 116
SfoI GGCGCC 1 cut(s) 318
SmiMI CAYNNNNRTG 1 cut(s) 95
SmlI CTYRAG 1 cut(s) 229
SmoI CTYRAG 1 cut(s) 229
Sse9I AATT 2 cut(s) 178, 416
SsiI CCGC 4 cut(s) 47, 102, 283, 346
SspDI GGCGCC 1 cut(s) 316
StyD4I CCNGG 1 cut(s) 136
StyI CCWWGG 1 cut(s) 73
TaaI ACNGT 1 cut(s) 435
TasI AATT 2 cut(s) 178, 416
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TspDTI ATGAA 3 cut(s) 44, 298, 339
XapI RAATTY 1 cut(s) 416
XceI RCATGY 2 cut(s) 100, 295
XcmI CCANNNNNNNNNTGG 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.