RchiOBHm_Chr7g0193641

mitogen-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0017483
Physical Location & Seq
Reverse (-)
11923995 .. 11925000
1006 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17316

Sequence Viewer

Length: 318 bp
ATGCTTCCTTCTTCACAACAAAAATTCAGAGATATCTATGTTGTGTTTGAGTTGATGGAATCGTACCTTCACCGGGTTATCAAGGCAAATGATGGTCTTACCCAGGAGCATTATCAGTTTTTCCTGTACCAGGTTCTTCGTGGTTTAAAGTATATTCATACAGCAAATGATTATGTTGCAACTCGATGGTACCGAGCCTCTGAACTTTGTGCCTCATTTTTCTCAAAATTTGATCTCATGTTGGAGAGGGTCGGACTATTGGACCAAGTGCTGCTGAAGTACTTAGCTTTTTCATGTATTAATGATCCACTGAATTGA

Protein Analysis

105

Amino Acids

12.5

Weight (kDa)

6.81

Isoelectric Point (pI)

31.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 7 - 55 1.1e-06 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 189
AccB1I GGYRCC 1 cut(s) 189
AclWI GGATC 1 cut(s) 299
AcsI RAATTY 2 cut(s) 23, 227
AcuI CTGAAG 1 cut(s) 296
AfaI GTAC 4 cut(s) 65, 128, 191, 281
AfiI CCNNNNNNNGG 2 cut(s) 73, 130
AjnI CCWGG 2 cut(s) 102, 129
AluBI AGCT 1 cut(s) 287
AluI AGCT 1 cut(s) 287
AlwI GGATC 1 cut(s) 299
ApeKI GCWGC 1 cut(s) 271
ApoI RAATTY 2 cut(s) 23, 227
AseI ATTAAT 1 cut(s) 300
Asp718I GGTACC 1 cut(s) 189
AspS9I GGNCC 1 cut(s) 262
AsuC2I CCSGG 1 cut(s) 74
AsuHPI GGTGA 1 cut(s) 62
AvaII GGWCC 1 cut(s) 262
BanI GGYRCC 1 cut(s) 189
BbvI GCAGC 1 cut(s) 258
BccI CCATC 3 cut(s) 49, 86, 180
BciT130I CCWGG 2 cut(s) 104, 131
BcnI CCSGG 1 cut(s) 74
BisI GCNGC 1 cut(s) 272
BlsI GCNGC 1 cut(s) 273
BmcAI AGTACT 1 cut(s) 281
Bme1390I CCNGG 3 cut(s) 74, 104, 131
Bme18I GGWCC 1 cut(s) 262
BmgT120I GGNCC 1 cut(s) 262
BmiI GGNNCC 1 cut(s) 191
BmrFI CCNGG 3 cut(s) 74, 104, 131
BpuMI CCSGG 1 cut(s) 74
BsaJI CCNNGG 1 cut(s) 102
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 130
BseBI CCWGG 2 cut(s) 104, 131
BseDI CCNNGG 1 cut(s) 102
BseLI CCNNNNNNNGG 2 cut(s) 73, 130
BseXI GCAGC 1 cut(s) 258
BshNI GGYRCC 1 cut(s) 189
BsiSI CCGG 1 cut(s) 73
BslI CCNNNNNNNGG 2 cut(s) 73, 130
Bsp143I GATC 2 cut(s) 232, 304
BspLI GGNNCC 1 cut(s) 191
BspPI GGATC 1 cut(s) 299
BspT107I GGYRCC 1 cut(s) 189
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 2 cut(s) 232, 304
Bst2UI CCWGG 2 cut(s) 104, 131
BstDEI CTNAG 1 cut(s) 283
BstENI CCTNNNNNAGG 1 cut(s) 128
BstKTI GATC 2 cut(s) 235, 307
BstMBI GATC 2 cut(s) 232, 304
BstNI CCWGG 2 cut(s) 104, 131
BstSCI CCNGG 3 cut(s) 72, 102, 129
BstV1I GCAGC 1 cut(s) 258
BtsIMutI CAGTG 1 cut(s) 308
Cfr13I GGNCC 1 cut(s) 262
CsiI ACCWGGT 1 cut(s) 129
Csp6I GTAC 4 cut(s) 64, 127, 190, 280
CviAII CATG 2 cut(s) 238, 294
CviJI RGCY 2 cut(s) 197, 287
CviKI_1 RGCY 2 cut(s) 197, 287
CviQI GTAC 4 cut(s) 64, 127, 190, 280
DdeI CTNAG 1 cut(s) 283
DpnI GATC 2 cut(s) 234, 306
DpnII GATC 2 cut(s) 232, 304
DraI TTTAAA 1 cut(s) 147
Eco32I GATATC 1 cut(s) 34
Eco47I GGWCC 1 cut(s) 262
Eco57I CTGAAG 1 cut(s) 296
EcoNI CCTNNNNNAGG 1 cut(s) 128
EcoRII CCWGG 2 cut(s) 102, 129
EcoRV GATATC 1 cut(s) 34
FaeI CATG 2 cut(s) 241, 297
FaiI YATR 6 cut(s) 39, 153, 159, 174, 239, 295
FatI CATG 2 cut(s) 237, 293
Fnu4HI GCNGC 1 cut(s) 272
Fsp4HI GCNGC 1 cut(s) 272
GluI GCNGC 1 cut(s) 272
HapII CCGG 1 cut(s) 73
Hin1II CATG 2 cut(s) 241, 297
HinfI GANTC 1 cut(s) 59
HpaII CCGG 1 cut(s) 73
HphI GGTGA 1 cut(s) 62
Hpy188I TCNGA 3 cut(s) 29, 202, 254
HpyAV CCTTC 2 cut(s) 18, 77
HpyCH4V TGCA 1 cut(s) 179
HpyF3I CTNAG 1 cut(s) 283
Hsp92II CATG 2 cut(s) 241, 297
KpnI GGTACC 1 cut(s) 193
Kzo9I GATC 2 cut(s) 232, 304
LmnI GCTCC 1 cut(s) 106
LpnPI CCDG 6 cut(s) 86, 89, 116, 116, 137, 143
Lsp1109I GCAGC 1 cut(s) 258
MabI ACCWGGT 1 cut(s) 129
MalI GATC 2 cut(s) 234, 306
MboI GATC 2 cut(s) 232, 304
MboII GAAGA 2 cut(s) 3, 128
MluCI AATT 3 cut(s) 23, 227, 313
MmeI TCCRAC 2 cut(s) 222, 232
MnlI CCTC 3 cut(s) 208, 223, 240
MseI TTAA 2 cut(s) 146, 300
MspI CCGG 1 cut(s) 73
MspR9I CCNGG 3 cut(s) 74, 104, 131
MvaI CCWGG 2 cut(s) 104, 131
NciI CCSGG 1 cut(s) 74
NdeII GATC 2 cut(s) 232, 304
NlaIII CATG 2 cut(s) 241, 297
NlaIV GGNNCC 1 cut(s) 191
PcsI WCGNNNNNNNCGW 1 cut(s) 190
PfeI GAWTC 1 cut(s) 59
PkrI GCNGC 1 cut(s) 273
PshBI ATTAAT 1 cut(s) 300
Psp6I CCWGG 2 cut(s) 102, 129
PspGI CCWGG 2 cut(s) 102, 129
PspN4I GGNNCC 1 cut(s) 191
PspPI GGNCC 1 cut(s) 262
RsaI GTAC 4 cut(s) 65, 128, 191, 281
RsaNI GTAC 4 cut(s) 64, 127, 190, 280
SaqAI TTAA 2 cut(s) 146, 300
SatI GCNGC 1 cut(s) 272
Sau3AI GATC 2 cut(s) 232, 304
Sau96I GGNCC 1 cut(s) 262
ScaI AGTACT 1 cut(s) 281
ScrFI CCNGG 3 cut(s) 74, 104, 131
SetI ASST 3 cut(s) 69, 135, 289
SexAI ACCWGGT 1 cut(s) 129
SinI GGWCC 1 cut(s) 262
Sse9I AATT 3 cut(s) 23, 227, 313
StyD4I CCNGG 3 cut(s) 72, 102, 129
TaqI TCGA 1 cut(s) 184
TasI AATT 3 cut(s) 23, 227, 313
TatI WGTACW 1 cut(s) 279
TfiI GAWTC 1 cut(s) 59
Tru1I TTAA 2 cut(s) 146, 300
Tru9I TTAA 2 cut(s) 146, 300
TscAI CASTG 1 cut(s) 315
TseI GCWGC 1 cut(s) 271
TspDTI ATGAA 2 cut(s) 146, 282
TspRI CASTG 1 cut(s) 315
VpaK11BI GGWCC 1 cut(s) 262
VspI ATTAAT 1 cut(s) 300
XagI CCTNNNNNAGG 1 cut(s) 128
XapI RAATTY 2 cut(s) 23, 227
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
ZrmI AGTACT 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.