Rroxscaffold_1G00050280

mitogen-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0017483
Physical Location & Seq
Reverse (-)
70842300 .. 70847276
4977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00050280.1

Sequence Viewer

Length: 399 bp
ATGGGTGGACTTGCTCTAAGCGCCACAAACAATCTAGAGTCGAATTCTTCACGAATATGGAAAGGCAAGCGGTACAAAGTTCGGGAGGTCATTGGGAAAGGAAGTTATGGTGTTATGGGTTCTGCGGTTGACACCCACAACAGAGAAAAAGTTGCAATCAAGAAGATTAATGGTGTCTTTGAGCATGCCTCGGATGATACAAGGATCCCGAGAGAAATGAAGCTTCTTCATCCGCTTCATCATCCCGATATTTTCAATGCTATGATTTATGCTAGACCTGTTCGAAGTTGCACAGCTTGTACACTTGTGTTTGGTGAAAGACGAGTTATATTGTTCATCAAACATTCATTACAAATTAGCTTCATTCGGGATGTAGCGATGATTCCAAACATGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

132

Amino Acids

14.87

Weight (kDa)

10.01

Isoelectric Point (pI)

33.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 25 - 87 2.8e-09 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 70, 125, 233
AclWI GGATC 2 cut(s) 199, 212
AcsI RAATTY 1 cut(s) 43
AfaI GTAC 2 cut(s) 74, 301
AgsI TTSAA 1 cut(s) 256
AluBI AGCT 3 cut(s) 223, 296, 360
AluI AGCT 3 cut(s) 223, 296, 360
AlwI GGATC 2 cut(s) 199, 212
Ama87I CYCGRG 1 cut(s) 208
ApoI RAATTY 1 cut(s) 43
AseI ATTAAT 1 cut(s) 168
AspLEI GCGC 1 cut(s) 23
AsuHPI GGTGA 1 cut(s) 326
AsuII TTCGAA 1 cut(s) 283
AvaI CYCGRG 1 cut(s) 208
BamHI GGATCC 1 cut(s) 204
BfaI CTAG 2 cut(s) 35, 273
BfoI RGCGCY 1 cut(s) 24
BmeT110I CYCGRG 1 cut(s) 208
BmiI GGNNCC 1 cut(s) 206
BplI GAGNNNNNCTC 2 cut(s) 173, 205
Bpu14I TTCGAA 1 cut(s) 283
BsaJI CCNNGG 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 189
BseGI GGATG 4 cut(s) 199, 229, 241, 376
BsiHKCI CYCGRG 1 cut(s) 208
BsoBI CYCGRG 1 cut(s) 208
Bsp119I TTCGAA 1 cut(s) 283
Bsp1407I TGTACA 1 cut(s) 299
Bsp143I GATC 1 cut(s) 204
BspACI CCGC 3 cut(s) 70, 125, 233
BspLI GGNNCC 1 cut(s) 206
BspPI GGATC 2 cut(s) 199, 212
BspT104I TTCGAA 1 cut(s) 283
BsrGI TGTACA 1 cut(s) 299
BssECI CCNNGG 1 cut(s) 189
BssMI GATC 1 cut(s) 204
BstAUI TGTACA 1 cut(s) 299
BstBI TTCGAA 1 cut(s) 283
BstC8I GCNNGC 2 cut(s) 68, 186
BstDEI CTNAG 1 cut(s) 17
BstF5I GGATG 4 cut(s) 199, 229, 241, 376
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 1 cut(s) 207
BstMBI GATC 1 cut(s) 204
BstMWI GCNNNNNNNGC 1 cut(s) 20
BstNSI RCATGY 2 cut(s) 188, 394
BstX2I RGATCY 1 cut(s) 204
BstYI RGATCY 1 cut(s) 204
BtgZI GCGATG 1 cut(s) 392
BtsCI GGATG 4 cut(s) 199, 229, 241, 376
Cac8I GCNNGC 2 cut(s) 68, 186
CfoI GCGC 1 cut(s) 23
Csp6I GTAC 2 cut(s) 73, 300
CviAII CATG 2 cut(s) 185, 391
CviJI RGCY 3 cut(s) 223, 296, 360
CviKI_1 RGCY 3 cut(s) 223, 296, 360
CviQI GTAC 2 cut(s) 73, 300
DdeI CTNAG 1 cut(s) 17
DpnI GATC 1 cut(s) 206
DpnII GATC 1 cut(s) 204
Eco88I CYCGRG 1 cut(s) 208
EcoRI GAATTC 1 cut(s) 43
FaeI CATG 2 cut(s) 188, 394
FaiI YATR 8 cut(s) 58, 108, 116, 186, 263, 270, 329, 392
FatI CATG 2 cut(s) 184, 390
FokI GGATG 4 cut(s) 206, 216, 228, 383
FspBI CTAG 2 cut(s) 35, 273
GlaI GCGC 1 cut(s) 22
HaeII RGCGCY 1 cut(s) 24
HhaI GCGC 1 cut(s) 23
Hin1II CATG 2 cut(s) 188, 394
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 1 cut(s) 221
HinfI GANTC 2 cut(s) 38, 382
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 3 cut(s) 8, 130, 302
Hpy188I TCNGA 1 cut(s) 193
Hpy188III TCNNGA 7 cut(s) 35, 51, 83, 160, 208, 245, 368
Hpy8I GTNNAC 3 cut(s) 8, 130, 302
HpyCH4V TGCA 2 cut(s) 155, 291
HpyF10VI GCNNNNNNNGC 1 cut(s) 20
HpyF3I CTNAG 1 cut(s) 17
Hsp92II CATG 2 cut(s) 188, 394
HspAI GCGC 1 cut(s) 21
Kzo9I GATC 1 cut(s) 204
LpnPI CCDG 1 cut(s) 291
MaeI CTAG 2 cut(s) 35, 273
MalI GATC 1 cut(s) 206
MboI GATC 1 cut(s) 204
MboII GAAGA 3 cut(s) 39, 175, 218
MflI RGATCY 1 cut(s) 204
MluCI AATT 2 cut(s) 43, 354
MlyI GAGTC 1 cut(s) 47
MnlI CCTC 2 cut(s) 79, 199
MseI TTAA 1 cut(s) 168
MslI CAYNNNNRTG 1 cut(s) 55
MwoI GCNNNNNNNGC 1 cut(s) 20
NdeII GATC 1 cut(s) 204
NlaIII CATG 2 cut(s) 188, 394
NlaIV GGNNCC 1 cut(s) 206
NspI RCATGY 2 cut(s) 188, 394
NspV TTCGAA 1 cut(s) 283
PaeI GCATGC 1 cut(s) 188
PfeI GAWTC 1 cut(s) 382
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
PshBI ATTAAT 1 cut(s) 168
PspN4I GGNNCC 1 cut(s) 206
PsuI RGATCY 1 cut(s) 204
RsaI GTAC 2 cut(s) 74, 301
RsaNI GTAC 2 cut(s) 73, 300
RseI CAYNNNNRTG 1 cut(s) 55
SaqAI TTAA 1 cut(s) 168
Sau3AI GATC 1 cut(s) 204
SchI GAGTC 1 cut(s) 47
SetI ASST 5 cut(s) 90, 225, 280, 298, 362
SfuI TTCGAA 1 cut(s) 283
SmiMI CAYNNNNRTG 1 cut(s) 55
SphI GCATGC 1 cut(s) 188
Sse9I AATT 2 cut(s) 43, 354
SsiI CCGC 3 cut(s) 70, 125, 233
SspMI CTAG 2 cut(s) 35, 273
TaqI TCGA 2 cut(s) 41, 283
TasI AATT 2 cut(s) 43, 354
TatI WGTACW 1 cut(s) 299
TfiI GAWTC 1 cut(s) 382
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TspDTI ATGAA 6 cut(s) 218, 227, 233, 325, 336, 352
VspI ATTAAT 1 cut(s) 168
XapI RAATTY 1 cut(s) 43
XbaI TCTAGA 1 cut(s) 34
XceI RCATGY 2 cut(s) 188, 394
XspI CTAG 2 cut(s) 35, 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.