RCHIOBHM_MTG0498431

cytochrome c biogenesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Mt
OG0018763
Physical Location & Seq
Forward (+)
78351 .. 80839
2489 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15614

Sequence Viewer

Length: 1347 bp
ATGGTCCAACTACATAACTTTTTCTTTTTCATTACTTCCATGGTCGTGCCTTGTGGCACGGCAGCACCCGTACTATTGAAATGGTTTGTCAGTAGAGATGTTCCCACAGGTGCCCCTTCTTCCAATGGTACTATAATTCCTATTCCTATCCCTGCATTCCCTCTTTTGGTCTATATACATTCCAGGAAATTCATACGCTCCATGGACGGAGCAAAAAGTGGAGTCTTGGTCAGAGCAAGCCGCCCTATTTTATTACCAGACATAATTGAGAGAGGCTCAGCCGAAATTAGAGCTAGAAACGCCTTATTTCGTTTCGTTCCCGTTCTTCATTTCCTTCTTCTCGCATACAAGGGGGACTTATCATATTTAGAATCTCTCTGCGGTGTGCTCTGTTTACTATTCTTTCGTACTCTCTTCTCTTTACCACGCGATAGGTCGTCAGCGAAGCGTGAGCGGGCGCGGAGAAGGAAACGCCAAACACTTCGGCCTAACGGGAATGAGCAACGACGAAATGACAAGATGAGGTGCTCCGGGCACCCCCGAAGGGTAGAAGGTTTTGGGCCTGTAGCTTTCCCCGCCCCCCCTTCGTCGGGTGGCGCTTGTGGGGGGCGTGTGCCACCAGAAATCGGGCTTGAAGCTCTCGCCTTACCAACGAGCCGACAGCTGATGGCTGTTGGTCACGACTACTACCAAAAAGCTCCAATGAAGATGAATATTTCACATGGAGGAGTGTGCATCTGTATGTTGGGTGTTCTTCTGTCGTCAACTAACACAAAGAAGATACAGTTCACTCAACGATTGCCTTTGGGTTCCGAACTCCTTATGGGGAAGGAGCGTTGTTGTTTGCGAGGTCTCGATCATTTACATGGACCCACTTTTCATTCCATTTGTGGAAATTTGATGATCTATAAACCGTCCCTAACGAACGATCGGCTCATGTTTGAGCATGATGAATCACTGCGTGCCGACGTGTTGCCAATAAGCTTTCCGGCCTCATATGAGAATGGAAAACTGGAGCATTTTCTGCATCGGTGGATGAAGAATCGCGAACATAATAATTTCTGGTTGACCATGTTCCCAGAAAAAAGATACTTTCGAGAAACGACGAGCACGACCGAAGTGGCTATACATACAAATCTATTTACGGATCTATATGCTTCGATTGGAACTGGAAGTTCCAGAACAGGCGGCTGGTATACCACCATAATGAAACTGCCTTTTATTTTTTTTATTCGGATAGGATTTATGTTGGCTTCGTCGGGAGGCTCGCGTAGTTTGTTACGTCAGCTCCAAAAGGATAAGTTGCGTTGGAATCGAGAAAGTTCCGTGGAGTTCATAATTGCATAA

Protein Analysis

448

Amino Acids

50.65

Weight (kDa)

10.1

Isoelectric Point (pI)

51.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CcmF_C PF16327 351 - 426 2.3e-09 Cytochrome c-type biogenesis protein CcmF C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018763)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07815 ATMG00180
prunus_persica Prupe.5G026600_v2.0.a1
pyrus_communis pycom12401g00060
rosa_chinensis RchiOBHm_MTg0498431
rosa_laevigata RLG00000000022 RLG00000000465 RLG00000000504
rosa_samantha Rh1BG019000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 110, 534
AccBSI CCGCTC 1 cut(s) 454
AccI GTMKAC 1 cut(s) 1196
AccII CGCG 4 cut(s) 429, 460, 1047, 1270
AciI CCGC 6 cut(s) 241, 381, 454, 460, 576, 1188
AclWI GGATC 1 cut(s) 1155
AcsI RAATTY 2 cut(s) 188, 895
AdeI CACNNNGTG 1 cut(s) 962
AfaI GTAC 3 cut(s) 72, 130, 409
AfiI CCNNNNNNNGG 5 cut(s) 166, 544, 589, 590, 626
AflIII ACRYGT 1 cut(s) 969
AgsI TTSAA 2 cut(s) 79, 635
AjiI CACGTC 1 cut(s) 970
AjnI CCWGG 1 cut(s) 182
AluBI AGCT 7 cut(s) 293, 569, 638, 664, 698, 984, 1288
AluI AGCT 7 cut(s) 293, 569, 638, 664, 698, 984, 1288
Alw21I GWGCWC 3 cut(s) 390, 530, 1112
Alw26I GTCTC 1 cut(s) 857
AlwI GGATC 1 cut(s) 1155
AoxI GGCC 3 cut(s) 485, 560, 990
ApeKI GCWGC 1 cut(s) 62
ApoI RAATTY 2 cut(s) 188, 895
AspLEI GCGC 2 cut(s) 460, 599
AspS9I GGNCC 3 cut(s) 4, 560, 869
AsuC2I CCSGG 1 cut(s) 532
AvaII GGWCC 2 cut(s) 4, 869
BaeGI GKGCMC 2 cut(s) 115, 537
BanI GGYRCC 2 cut(s) 110, 534
BarI GAAGNNNNNNTAC 2 cut(s) 1110, 1142
Bbv12I GWGCWC 3 cut(s) 390, 530, 1112
BbvI GCAGC 1 cut(s) 74
BccI CCATC 1 cut(s) 661
BceAI ACGGC 1 cut(s) 75
BciT130I CCWGG 1 cut(s) 184
BcnI CCSGG 1 cut(s) 532
BcoDI GTCTC 1 cut(s) 857
BfaI CTAG 1 cut(s) 294
BfmI CTRYAG 1 cut(s) 564
BfoI RGCGCY 1 cut(s) 600
BisI GCNGC 3 cut(s) 63, 241, 1189
BlpI GCTNAGC 1 cut(s) 277
BlsI GCNGC 3 cut(s) 64, 242, 1190
Bme1390I CCNGG 2 cut(s) 184, 532
Bme18I GGWCC 2 cut(s) 4, 869
BmgBI CACGTC 1 cut(s) 970
BmgT120I GGNCC 3 cut(s) 4, 560, 869
BmiI GGNNCC 4 cut(s) 112, 536, 811, 871
BmrFI CCNGG 2 cut(s) 184, 532
BmsI GCATC 2 cut(s) 744, 1036
BplI GAGNNNNNCTC 2 cut(s) 260, 292
BpmI CTGGAG 1 cut(s) 1034
Bpu1102I GCTNAGC 1 cut(s) 277
BpuMI CCSGG 1 cut(s) 532
BsaI GGTCTC 1 cut(s) 857
BsaJI CCNNGG 3 cut(s) 39, 201, 1326
BsaXI ACNNNNNCTCC 4 cut(s) 213, 243, 1272, 1302
Bsc4I CCNNNNNNNGG 5 cut(s) 166, 544, 589, 590, 626
Bse1I ACTGG 2 cut(s) 1017, 1174
BseBI CCWGG 1 cut(s) 184
BseDI CCNNGG 3 cut(s) 39, 201, 1326
BseGI GGATG 1 cut(s) 1041
BseLI CCNNNNNNNGG 5 cut(s) 166, 544, 589, 590, 626
BseMII CTCAG 1 cut(s) 291
BseNI ACTGG 2 cut(s) 1017, 1174
BseRI GAGGAG 1 cut(s) 741
BseSI GKGCMC 2 cut(s) 115, 537
BseXI GCAGC 1 cut(s) 74
Bsh1236I CGCG 4 cut(s) 429, 460, 1047, 1270
Bsh1285I CGRYCG 2 cut(s) 931, 1116
BshFI GGCC 3 cut(s) 487, 562, 992
BshNI GGYRCC 2 cut(s) 110, 534
BsiEI CGRYCG 2 cut(s) 931, 1116
BsiHKAI GWGCWC 3 cut(s) 390, 530, 1112
BsiSI CCGG 2 cut(s) 531, 989
BslFI GGGAC 2 cut(s) 368, 901
BslI CCNNNNNNNGG 5 cut(s) 166, 544, 589, 590, 626
BsmAI GTCTC 1 cut(s) 857
BsmFI GGGAC 2 cut(s) 368, 901
BsmI GAATGC 1 cut(s) 155
BsnI GGCC 3 cut(s) 487, 562, 992
Bso31I GGTCTC 1 cut(s) 857
Bsp1286I GDGCHC 5 cut(s) 115, 390, 530, 537, 1112
Bsp143I GATC 4 cut(s) 856, 903, 928, 1147
Bsp1720I GCTNAGC 1 cut(s) 277
Bsp19I CCATGG 2 cut(s) 39, 201
Bsp68I TCGCGA 1 cut(s) 1047
BspACI CCGC 6 cut(s) 241, 381, 454, 460, 576, 1188
BspANI GGCC 3 cut(s) 487, 562, 992
BspCNI CTCAG 1 cut(s) 290
BspFNI CGCG 4 cut(s) 429, 460, 1047, 1270
BspLI GGNNCC 4 cut(s) 112, 536, 811, 871
BspPI GGATC 1 cut(s) 1155
BspT107I GGYRCC 2 cut(s) 110, 534
BspTNI GGTCTC 1 cut(s) 857
BsrBI CCGCTC 1 cut(s) 454
BsrI ACTGG 2 cut(s) 1017, 1174
BssECI CCNNGG 3 cut(s) 39, 201, 1326
BssMI GATC 4 cut(s) 856, 903, 928, 1147
BssNAI GTATAC 1 cut(s) 1197
BssT1I CCWWGG 2 cut(s) 39, 201
Bst1107I GTATAC 1 cut(s) 1197
Bst2UI CCWGG 1 cut(s) 184
Bst4CI ACNGT 2 cut(s) 786, 915
Bst6I CTCTTC 1 cut(s) 419
BstAPI GCANNNNNTGC 1 cut(s) 1024
BstC8I GCNNGC 4 cut(s) 238, 456, 963, 1268
BstDEI CTNAG 1 cut(s) 277
BstDSI CCRYGG 3 cut(s) 39, 201, 1326
BstF5I GGATG 1 cut(s) 1041
BstFNI CGCG 4 cut(s) 429, 460, 1047, 1270
BstH2I RGCGCY 1 cut(s) 600
BstHHI GCGC 2 cut(s) 460, 599
BstKTI GATC 4 cut(s) 859, 906, 931, 1150
BstMAI GTCTC 1 cut(s) 857
BstMBI GATC 4 cut(s) 856, 903, 928, 1147
BstMCI CGRYCG 2 cut(s) 931, 1116
BstMWI GCNNNNNNNGC 3 cut(s) 299, 575, 1024
BstNI CCWGG 1 cut(s) 184
BstSCI CCNGG 2 cut(s) 182, 530
BstSFI CTRYAG 1 cut(s) 564
BstSLI GKGCMC 2 cut(s) 115, 537
BstUI CGCG 4 cut(s) 429, 460, 1047, 1270
BstV1I GCAGC 1 cut(s) 74
BstX2I RGATCY 1 cut(s) 1147
BstYI RGATCY 1 cut(s) 1147
BstZ17I GTATAC 1 cut(s) 1197
BsuRI GGCC 3 cut(s) 487, 562, 992
BtgI CCRYGG 3 cut(s) 39, 201, 1326
BtrI CACGTC 1 cut(s) 970
BtsCI GGATG 1 cut(s) 1041
BtsI GCAGTG 1 cut(s) 956
BtsIMutI CAGTG 1 cut(s) 956
BtuMI TCGCGA 1 cut(s) 1047
Cac8I GCNNGC 4 cut(s) 238, 456, 963, 1268
CfoI GCGC 2 cut(s) 460, 599
Cfr13I GGNCC 3 cut(s) 4, 560, 869
Csp6I GTAC 3 cut(s) 71, 129, 408
CviAII CATG 7 cut(s) 40, 202, 722, 866, 937, 947, 1072
CviQI GTAC 3 cut(s) 71, 129, 408
DdeI CTNAG 1 cut(s) 277
DpnI GATC 4 cut(s) 858, 905, 930, 1149
DpnII GATC 4 cut(s) 856, 903, 928, 1147
DraIII CACNNNGTG 1 cut(s) 962
Eam1104I CTCTTC 1 cut(s) 419
EarI CTCTTC 1 cut(s) 419
Eco130I CCWWGG 2 cut(s) 39, 201
Eco31I GGTCTC 1 cut(s) 857
Eco47I GGWCC 2 cut(s) 4, 869
EcoRII CCWGG 1 cut(s) 182
EcoT14I CCWWGG 2 cut(s) 39, 201
ErhI CCWWGG 2 cut(s) 39, 201
FaeI CATG 7 cut(s) 43, 205, 725, 869, 940, 950, 1075
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FaqI GGGAC 2 cut(s) 368, 901
FatI CATG 7 cut(s) 39, 201, 721, 865, 936, 946, 1071
FauI CCCGC 2 cut(s) 447, 583
FauNDI CATATG 1 cut(s) 997
FblI GTMKAC 1 cut(s) 1196
Fnu4HI GCNGC 3 cut(s) 63, 241, 1189
FokI GGATG 1 cut(s) 1048
Fsp4HI GCNGC 3 cut(s) 63, 241, 1189
FspBI CTAG 1 cut(s) 294
GlaI GCGC 2 cut(s) 459, 598
GluI GCNGC 3 cut(s) 63, 241, 1189
GsuI CTGGAG 1 cut(s) 1034
HaeII RGCGCY 1 cut(s) 600
HaeIII GGCC 3 cut(s) 487, 562, 992
HapII CCGG 2 cut(s) 531, 989
HhaI GCGC 2 cut(s) 460, 599
Hin1II CATG 7 cut(s) 43, 205, 725, 869, 940, 950, 1075
Hin6I GCGC 2 cut(s) 458, 597
HinP1I GCGC 2 cut(s) 458, 597
HincII GTYRAC 2 cut(s) 765, 1068
HindII GTYRAC 2 cut(s) 765, 1068
HindIII AAGCTT 1 cut(s) 982
HinfI GANTC 5 cut(s) 222, 371, 953, 1042, 1312
HpaII CCGG 2 cut(s) 531, 989
Hpy166II GTNNAC 5 cut(s) 395, 765, 789, 1068, 1197
Hpy188I TCNGA 3 cut(s) 233, 814, 1236
Hpy188III TCNNGA 7 cut(s) 680, 854, 1046, 1097, 1179, 1260, 1316
Hpy8I GTNNAC 5 cut(s) 395, 765, 789, 1068, 1197
Hpy99I CGWCG 5 cut(s) 510, 592, 971, 1108, 1261
HpyAV CCTTC 7 cut(s) 126, 344, 459, 537, 545, 594, 823
HpyCH4III ACNGT 2 cut(s) 786, 915
HpyCH4IV ACGT 2 cut(s) 969, 1282
HpyCH4V TGCA 4 cut(s) 155, 735, 1027, 1343
HpyF10VI GCNNNNNNNGC 3 cut(s) 299, 575, 1024
HpyF3I CTNAG 1 cut(s) 277
HpySE526I ACGT 2 cut(s) 969, 1282
Hsp92II CATG 7 cut(s) 43, 205, 725, 869, 940, 950, 1075
HspAI GCGC 2 cut(s) 458, 597
Kzo9I GATC 4 cut(s) 856, 903, 928, 1147
LmnI GCTCC 7 cut(s) 203, 209, 533, 703, 832, 1015, 1293
Lsp1109I GCAGC 1 cut(s) 74
LweI GCATC 2 cut(s) 744, 1036
MaeI CTAG 1 cut(s) 294
MaeII ACGT 2 cut(s) 969, 1282
MaeIII GTNAC 2 cut(s) 677, 1278
MalI GATC 4 cut(s) 858, 905, 930, 1149
MbiI CCGCTC 1 cut(s) 454
MboI GATC 4 cut(s) 856, 903, 928, 1147
MboII GAAGA 8 cut(s) 111, 317, 329, 406, 718, 746, 790, 1051
MflI RGATCY 1 cut(s) 1147
MhlI GDGCHC 5 cut(s) 115, 390, 530, 537, 1112
MluCI AATT 7 cut(s) 135, 188, 264, 285, 895, 1057, 1338
MlyI GAGTC 1 cut(s) 231
MmeI TCCRAC 2 cut(s) 31, 1289
MnlI CCTC 7 cut(s) 171, 266, 516, 719, 842, 1003, 1256
MslI CAYNNNNRTG 4 cut(s) 44, 740, 864, 1205
MspA1I CMGCKG 1 cut(s) 664
MspI CCGG 2 cut(s) 531, 989
MspR9I CCNGG 2 cut(s) 184, 532
Mva1269I GAATGC 1 cut(s) 155
MvaI CCWGG 1 cut(s) 184
MvnI CGCG 4 cut(s) 429, 460, 1047, 1270
MwoI GCNNNNNNNGC 3 cut(s) 299, 575, 1024
NciI CCSGG 1 cut(s) 532
NcoI CCATGG 2 cut(s) 39, 201
NdeI CATATG 1 cut(s) 997
NdeII GATC 4 cut(s) 856, 903, 928, 1147
NlaIII CATG 7 cut(s) 43, 205, 725, 869, 940, 950, 1075
NlaIV GGNNCC 4 cut(s) 112, 536, 811, 871
NmuCI GTSAC 1 cut(s) 677
NruI TCGCGA 1 cut(s) 1047
PcsI WCGNNNNNNNCGW 1 cut(s) 1109
PctI GAATGC 1 cut(s) 155
PfeI GAWTC 4 cut(s) 371, 953, 1042, 1312
PfoI TCCNGGA 1 cut(s) 182
PkrI GCNGC 3 cut(s) 64, 242, 1190
Ple19I CGATCG 1 cut(s) 931
PleI GAGTC 1 cut(s) 230
PpsI GAGTC 1 cut(s) 230
Psp6I CCWGG 1 cut(s) 182
PspGI CCWGG 1 cut(s) 182
PspN4I GGNNCC 4 cut(s) 112, 536, 811, 871
PspPI GGNCC 3 cut(s) 4, 560, 869
PsuI RGATCY 1 cut(s) 1147
PvuI CGATCG 1 cut(s) 931
PvuII CAGCTG 1 cut(s) 664
RruI TCGCGA 1 cut(s) 1047
RsaI GTAC 3 cut(s) 72, 130, 409
RsaNI GTAC 3 cut(s) 71, 129, 408
RseI CAYNNNNRTG 4 cut(s) 44, 740, 864, 1205
SatI GCNGC 3 cut(s) 63, 241, 1189
Sau3AI GATC 4 cut(s) 856, 903, 928, 1147
Sau96I GGNCC 3 cut(s) 4, 560, 869
SchI GAGTC 1 cut(s) 231
ScrFI CCNGG 2 cut(s) 184, 532
SduI GDGCHC 5 cut(s) 115, 390, 530, 537, 1112
SfaNI GCATC 2 cut(s) 744, 1036
SfcI CTRYAG 1 cut(s) 564
SinI GGWCC 2 cut(s) 4, 869
SmiMI CAYNNNNRTG 4 cut(s) 44, 740, 864, 1205
Sse9I AATT 7 cut(s) 135, 188, 264, 285, 895, 1057, 1338
SsiI CCGC 6 cut(s) 241, 381, 454, 460, 576, 1188
SspI AATATT 1 cut(s) 715
SspMI CTAG 1 cut(s) 294
StyD4I CCNGG 2 cut(s) 182, 530
StyI CCWWGG 2 cut(s) 39, 201
TaaI ACNGT 2 cut(s) 786, 915
TaiI ACGT 2 cut(s) 972, 1285
TaqI TCGA 4 cut(s) 855, 1096, 1160, 1315
TaqII GACCGA 1 cut(s) 1130
TasI AATT 7 cut(s) 135, 188, 264, 285, 895, 1057, 1338
TauI GCSGC 2 cut(s) 243, 1191
TfiI GAWTC 4 cut(s) 371, 953, 1042, 1312
TscAI CASTG 1 cut(s) 963
TseFI GTSAC 1 cut(s) 677
TseI GCWGC 1 cut(s) 62
Tsp45I GTSAC 1 cut(s) 677
TspGWI ACGGA 3 cut(s) 222, 1160, 1315
TspRI CASTG 1 cut(s) 963
VpaK11BI GGWCC 2 cut(s) 4, 869
XapI RAATTY 2 cut(s) 188, 895
XmiI GTMKAC 1 cut(s) 1196
XspI CTAG 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.