RLG00000000459

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0019978
Physical Location & Seq
Reverse (-)
1049140 .. 1050696
1557 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000459

Sequence Viewer

Length: 858 bp
ATGATACAAATACTCCATGATGCTCGTCTACCAATTCATAGGAGGCAAAGACAAAAGAAAGAGCAAAGGAACTCAGACTCACCTAGTGAGTATATGGATTTCGTGTCTAAGTTTCCAATAACTCTCAAGGAGTTTATGCCACCTGAGTTAATGGACATCCCAGGCGTTGAAAGCCTGTCATGTCAGTGTACGACGGAGAAGGAAAGCCATACGAACATTTGGTACTCTTCCTTCATCGATGTGGAGATAGAGCCAGAAAAGGGAACCACGGGGATTCGAAATGTTGAATACATCGGAAATGGTCAGGAAGAAGAGGAGCTGTTAGGAATAGAAAAGGACAAAGCCTTGAAAGTCCCGCTTATGTTGAATTCGGAACACATGGCTCCTTATCCAGAATGGATGGAACATGTCCCATATCCTCCTTCATACCAAATCCCTAACTTTTTCCGGTTTAATGCCGGAGATGACGCATTGGCTTATTTTGTGTATTTTCAACAAGAGTGTGGGGTAAGAGCTGCTGTCGAGGCTCTCTGTTGCAAACTATTTCGTTACTCGCTTATGGGAAGAACCCCTATTTGGTATGATTACTTCGCCTGGGGATCCATACCCATACGGGAGCTATGGTGTTTTCTCCAAGAGTTAGGAGCACTCAAGGATGGAATATTCCAAATCATGAATGGGGTGACTCCGATGATGATTGGGACTTCCAATCTGAACAAGACAAGACCGATGGTTCTCATACTTCTTCAGTCTCAGGGGATGTGGGGAGTGCAGCACCTGACGGCTGGACCTCTGAAGTTGATGAATTTTTTGAAGGTAGAGTCTAGTCTTATCATGGCAGATGGTTCAGATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

286

Amino Acids

32.83

Weight (kDa)

5.16

Isoelectric Point (pI)

47.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019978)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_MTg0498561
rosa_laevigata RLG00000000459
rosa_multiflora Rmu_sc0003129.1_g000010
rosa_roxburghii Rroxscaffold_2G00119260 Rroxscaffold_3G00257290
rosa_samantha Rh2AG001000 Rh2CG000800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 28
AciI CCGC 1 cut(s) 356
AclWI GGATC 2 cut(s) 594, 607
AcsI RAATTY 2 cut(s) 367, 805
AcuI CTGAAG 2 cut(s) 731, 815
AdeI CACNNNGTG 1 cut(s) 86
AfaI GTAC 2 cut(s) 190, 224
AfiI CCNNNNNNNGG 2 cut(s) 260, 576
AflIII ACRYGT 1 cut(s) 406
AgsI TTSAA 6 cut(s) 170, 287, 349, 367, 494, 814
AjnI CCWGG 2 cut(s) 160, 593
AjuI GAANNNNNNNTTGG 2 cut(s) 559, 591
AluBI AGCT 3 cut(s) 319, 515, 619
AluI AGCT 3 cut(s) 319, 515, 619
Alw21I GWGCWC 1 cut(s) 649
Alw26I GTCTC 1 cut(s) 756
AlwI GGATC 2 cut(s) 594, 607
AlwNI CAGNNNCTG 1 cut(s) 778
ApeKI GCWGC 2 cut(s) 515, 772
ApoI RAATTY 2 cut(s) 367, 805
AspS9I GGNCC 1 cut(s) 788
AsuHPI GGTGA 2 cut(s) 72, 694
AsuII TTCGAA 1 cut(s) 277
AvaII GGWCC 1 cut(s) 788
BamHI GGATCC 1 cut(s) 599
BarI GAAGNNNNNNTAC 4 cut(s) 215, 247, 572, 604
Bbv12I GWGCWC 1 cut(s) 649
BbvI GCAGC 2 cut(s) 502, 784
BccI CCATC 4 cut(s) 394, 650, 724, 836
BceAI ACGGC 1 cut(s) 798
BciT130I CCWGG 2 cut(s) 162, 595
BcoDI GTCTC 1 cut(s) 756
BfaI CTAG 2 cut(s) 84, 825
BisI GCNGC 2 cut(s) 516, 773
BlsI GCNGC 2 cut(s) 517, 774
Bme1390I CCNGG 2 cut(s) 162, 595
Bme18I GGWCC 1 cut(s) 788
BmgT120I GGNCC 1 cut(s) 788
BmiI GGNNCC 3 cut(s) 265, 384, 601
BmrFI CCNGG 2 cut(s) 162, 595
BmsI GCATC 1 cut(s) 10
Bpu14I TTCGAA 1 cut(s) 277
BpuEI CTTGAG 2 cut(s) 110, 635
Bsa29I ATCGAT 1 cut(s) 237
BsaJI CCNNGG 3 cut(s) 160, 267, 594
BsaWI WCCGGW 1 cut(s) 447
BsaXI ACNNNNNCTCC 3 cut(s) 27, 608, 638
Bsc4I CCNNNNNNNGG 2 cut(s) 260, 576
BseBI CCWGG 2 cut(s) 162, 595
BseCI ATCGAT 1 cut(s) 237
BseDI CCNNGG 3 cut(s) 160, 267, 594
BseGI GGATG 4 cut(s) 156, 405, 661, 765
BseLI CCNNNNNNNGG 2 cut(s) 260, 576
BseMII CTCAG 3 cut(s) 87, 135, 767
BseRI GAGGAG 1 cut(s) 329
BseXI GCAGC 2 cut(s) 502, 784
BsgI GTGCAG 1 cut(s) 791
BshVI ATCGAT 1 cut(s) 237
BsiHKAI GWGCWC 1 cut(s) 649
BsiSI CCGG 2 cut(s) 448, 459
BslFI GGGAC 3 cut(s) 338, 395, 715
BslI CCNNNNNNNGG 2 cut(s) 260, 576
BsmAI GTCTC 1 cut(s) 756
BsmFI GGGAC 3 cut(s) 338, 395, 715
Bsp119I TTCGAA 1 cut(s) 277
Bsp1286I GDGCHC 1 cut(s) 649
Bsp143I GATC 1 cut(s) 599
BspACI CCGC 1 cut(s) 356
BspCNI CTCAG 3 cut(s) 86, 136, 766
BspDI ATCGAT 1 cut(s) 237
BspHI TCATGA 1 cut(s) 672
BspLI GGNNCC 3 cut(s) 265, 384, 601
BspPI GGATC 2 cut(s) 594, 607
BspT104I TTCGAA 1 cut(s) 277
BssECI CCNNGG 3 cut(s) 160, 267, 594
BssMI GATC 1 cut(s) 599
Bst2UI CCWGG 2 cut(s) 162, 595
Bst6I CTCTTC 2 cut(s) 232, 306
BstBI TTCGAA 1 cut(s) 277
BstDEI CTNAG 4 cut(s) 73, 108, 144, 753
BstDSI CCRYGG 1 cut(s) 267
BstF5I GGATG 4 cut(s) 156, 405, 661, 765
BstKTI GATC 1 cut(s) 602
BstMAI GTCTC 1 cut(s) 756
BstMBI GATC 1 cut(s) 599
BstMWI GCNNNNNNNGC 2 cut(s) 171, 524
BstNI CCWGG 2 cut(s) 162, 595
BstNSI RCATGY 1 cut(s) 410
BstSCI CCNGG 2 cut(s) 160, 593
BstV1I GCAGC 2 cut(s) 502, 784
BstX2I RGATCY 1 cut(s) 599
BstYI RGATCY 1 cut(s) 599
Bsu15I ATCGAT 1 cut(s) 237
BsuTUI ATCGAT 1 cut(s) 237
BtgI CCRYGG 1 cut(s) 267
BtsCI GGATG 4 cut(s) 156, 405, 661, 765
BtsIMutI CAGTG 1 cut(s) 191
CaiI CAGNNNCTG 1 cut(s) 778
CciI TCATGA 1 cut(s) 672
Cfr13I GGNCC 1 cut(s) 788
ClaI ATCGAT 1 cut(s) 237
CseI GACGC 1 cut(s) 476
Csp6I GTAC 2 cut(s) 189, 223
CviAII CATG 6 cut(s) 17, 180, 379, 407, 673, 835
CviQI GTAC 2 cut(s) 189, 223
DdeI CTNAG 4 cut(s) 73, 108, 144, 753
DpnI GATC 1 cut(s) 601
DpnII GATC 1 cut(s) 599
DraIII CACNNNGTG 1 cut(s) 86
Eam1104I CTCTTC 2 cut(s) 232, 306
EarI CTCTTC 2 cut(s) 232, 306
Eco47I GGWCC 1 cut(s) 788
Eco57I CTGAAG 2 cut(s) 731, 815
EcoRI GAATTC 1 cut(s) 367
EcoRII CCWGG 2 cut(s) 160, 593
FaeI CATG 6 cut(s) 20, 183, 382, 410, 676, 838
FalI AAGNNNNNCTT 2 cut(s) 342, 374
FaqI GGGAC 3 cut(s) 338, 395, 715
FatI CATG 6 cut(s) 16, 179, 378, 406, 672, 834
FauI CCCGC 1 cut(s) 363
FblI GTMKAC 1 cut(s) 28
Fnu4HI GCNGC 2 cut(s) 516, 773
FokI GGATG 4 cut(s) 143, 412, 668, 772
Fsp4HI GCNGC 2 cut(s) 516, 773
FspBI CTAG 2 cut(s) 84, 825
GluI GCNGC 2 cut(s) 516, 773
HapII CCGG 2 cut(s) 448, 459
HgaI GACGC 1 cut(s) 476
Hin1II CATG 6 cut(s) 20, 183, 382, 410, 676, 838
HinfI GANTC 4 cut(s) 77, 274, 685, 821
HpaII CCGG 2 cut(s) 448, 459
HphI GGTGA 2 cut(s) 72, 694
Hpy166II GTNNAC 2 cut(s) 29, 189
Hpy188I TCNGA 8 cut(s) 76, 296, 373, 690, 714, 795, 850, 857
Hpy188III TCNNGA 3 cut(s) 305, 392, 673
Hpy8I GTNNAC 2 cut(s) 29, 189
Hpy99I CGWCG 1 cut(s) 196
HpyAV CCTTC 4 cut(s) 193, 241, 432, 808
HpyCH4V TGCA 2 cut(s) 537, 772
HpyF10VI GCNNNNNNNGC 2 cut(s) 171, 524
HpyF3I CTNAG 4 cut(s) 73, 108, 144, 753
Hsp92II CATG 6 cut(s) 20, 183, 382, 410, 676, 838
Kzo9I GATC 1 cut(s) 599
LmnI GCTCC 4 cut(s) 316, 388, 616, 644
Lsp1109I GCAGC 2 cut(s) 502, 784
LweI GCATC 1 cut(s) 10
MaeI CTAG 2 cut(s) 84, 825
MaeIII GTNAC 2 cut(s) 548, 682
MalI GATC 1 cut(s) 601
MboI GATC 1 cut(s) 599
MboII GAAGA 5 cut(s) 219, 320, 323, 576, 737
MflI RGATCY 1 cut(s) 599
MhlI GDGCHC 1 cut(s) 649
MluCI AATT 3 cut(s) 33, 367, 805
MlyI GAGTC 3 cut(s) 71, 679, 830
MnlI CCTC 5 cut(s) 36, 307, 429, 517, 801
MseI TTAA 2 cut(s) 149, 453
MslI CAYNNNNRTG 2 cut(s) 184, 239
MspI CCGG 2 cut(s) 448, 459
MspR9I CCNGG 2 cut(s) 162, 595
MvaI CCWGG 2 cut(s) 162, 595
MwoI GCNNNNNNNGC 2 cut(s) 171, 524
NdeII GATC 1 cut(s) 599
NlaIII CATG 6 cut(s) 20, 183, 382, 410, 676, 838
NlaIV GGNNCC 3 cut(s) 265, 384, 601
NmuCI GTSAC 1 cut(s) 682
NspI RCATGY 1 cut(s) 410
NspV TTCGAA 1 cut(s) 277
PagI TCATGA 1 cut(s) 672
PciI ACATGT 1 cut(s) 406
PfeI GAWTC 1 cut(s) 274
PkrI GCNGC 2 cut(s) 517, 774
PleI GAGTC 3 cut(s) 71, 679, 829
PpsI GAGTC 3 cut(s) 71, 679, 829
PscI ACATGT 1 cut(s) 406
Psp6I CCWGG 2 cut(s) 160, 593
PspGI CCWGG 2 cut(s) 160, 593
PspN4I GGNNCC 3 cut(s) 265, 384, 601
PspPI GGNCC 1 cut(s) 788
PsrI GAACNNNNNNTAC 2 cut(s) 206, 238
PstNI CAGNNNCTG 1 cut(s) 778
PsuI RGATCY 1 cut(s) 599
RsaI GTAC 2 cut(s) 190, 224
RsaNI GTAC 2 cut(s) 189, 223
RseI CAYNNNNRTG 2 cut(s) 184, 239
SaqAI TTAA 2 cut(s) 149, 453
SatI GCNGC 2 cut(s) 516, 773
Sau3AI GATC 1 cut(s) 599
Sau96I GGNCC 1 cut(s) 788
SchI GAGTC 3 cut(s) 71, 679, 830
ScrFI CCNGG 2 cut(s) 162, 595
SduI GDGCHC 1 cut(s) 649
SetI ASST 8 cut(s) 85, 145, 321, 517, 621, 780, 793, 819
SfaNI GCATC 1 cut(s) 10
SfuI TTCGAA 1 cut(s) 277
SinI GGWCC 1 cut(s) 788
SmiMI CAYNNNNRTG 2 cut(s) 184, 239
SmlI CTYRAG 2 cut(s) 125, 650
SmoI CTYRAG 2 cut(s) 125, 650
Sse9I AATT 3 cut(s) 33, 367, 805
SsiI CCGC 1 cut(s) 356
SspI AATATT 1 cut(s) 663
SspMI CTAG 2 cut(s) 84, 825
StyD4I CCNGG 2 cut(s) 160, 593
TaqI TCGA 3 cut(s) 237, 277, 522
TaqII GACCGA 1 cut(s) 742
TasI AATT 3 cut(s) 33, 367, 805
TfiI GAWTC 1 cut(s) 274
Tru1I TTAA 2 cut(s) 149, 453
Tru9I TTAA 2 cut(s) 149, 453
TscAI CASTG 1 cut(s) 191
TseFI GTSAC 1 cut(s) 682
TseI GCWGC 2 cut(s) 515, 772
Tsp45I GTSAC 1 cut(s) 682
TspDTI ATGAA 5 cut(s) 26, 223, 414, 689, 818
TspGWI ACGGA 1 cut(s) 209
TspRI CASTG 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 788
XapI RAATTY 2 cut(s) 367, 805
XceI RCATGY 1 cut(s) 410
XcmI CCANNNNNNNNNTGG 1 cut(s) 674
XmiI GTMKAC 1 cut(s) 28
XspI CTAG 2 cut(s) 84, 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.