RLG00000002257

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0017021
Physical Location & Seq
Reverse (-)
30802555 .. 30807242
4688 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002257

Sequence Viewer

Length: 480 bp
ATGACGCTCTGCTTGTTCAGCAAAACCAGCTGCTCCACTGAGATCCGACATCCTCATCTGCACTACCGACTAGTTCACCGCCACCTGCAACAGCTGGCTCCGGCTCAGCCTGGACAGCACCTCCACATCAACGGTGCCGCCGATGCTGCGTTTCAGGACTGGCAGGGAGTCGGCGGAGTCAGAGGCAACGATGGGCATGGCATGGAGGGCCGCGACTTGAGGCTGGAGGAATCGAACGTAGCGGTGCGCCGCCTCGAGCACGGTGGCAGTGTCCATCTTCTTCTCCCATGGTTCCTGACTCTAGGAGATAGTACGATTAGGGAGCAACTTATTGATGGTGGAAATGAGGAAAAGAAATTAGAGAATGGGACGGAGATAGTCGTTGATCAAAAGGAGGATAACTTGGAGGAAGATAGGGTGTTTCAATGGAATGGCGAATATTTGGACAGCTTCTTGCGTTTGAAGTTTTCCTCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

18.07

Weight (kDa)

5.63

Isoelectric Point (pI)

45.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 93
Acc36I ACCTGC 1 cut(s) 93
AccB1I GGYRCC 1 cut(s) 134
AccII CGCG 1 cut(s) 213
AciI CCGC 6 cut(s) 79, 138, 174, 211, 242, 250
AclWI GGATC 1 cut(s) 37
AfaI GTAC 1 cut(s) 313
AgsI TTSAA 2 cut(s) 425, 463
AhlI ACTAGT 1 cut(s) 70
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 3 cut(s) 30, 94, 450
AluI AGCT 3 cut(s) 30, 94, 450
Alw21I GWGCWC 1 cut(s) 261
AlwI GGATC 1 cut(s) 37
Ama87I CYCGRG 1 cut(s) 254
AoxI GGCC 1 cut(s) 208
ApeKI GCWGC 2 cut(s) 30, 146
AspLEI GCGC 1 cut(s) 249
AspS9I GGNCC 1 cut(s) 208
AsuHPI GGTGA 1 cut(s) 68
AvaI CYCGRG 1 cut(s) 254
BanI GGYRCC 1 cut(s) 134
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 2 cut(s) 17, 133
BccI CCATC 3 cut(s) 185, 282, 329
BciT130I CCWGG 1 cut(s) 111
BclI TGATCA 1 cut(s) 385
BcuI ACTAGT 1 cut(s) 70
BfaI CTAG 2 cut(s) 71, 302
BfuAI ACCTGC 1 cut(s) 93
BisI GCNGC 5 cut(s) 31, 138, 147, 211, 250
BlpI GCTNAGC 1 cut(s) 105
BlsI GCNGC 5 cut(s) 32, 139, 148, 212, 251
Bme1390I CCNGG 1 cut(s) 111
BmeT110I CYCGRG 1 cut(s) 254
BmgT120I GGNCC 1 cut(s) 208
BmiI GGNNCC 3 cut(s) 99, 136, 293
BmrFI CCNGG 1 cut(s) 111
BmsI GCATC 1 cut(s) 133
BpmI CTGGAG 1 cut(s) 245
Bpu1102I GCTNAGC 1 cut(s) 105
BpuEI CTTGAG 1 cut(s) 238
BsaJI CCNNGG 1 cut(s) 287
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bse1I ACTGG 1 cut(s) 164
BseBI CCWGG 1 cut(s) 111
BseDI CCNNGG 1 cut(s) 287
BseGI GGATG 1 cut(s) 49
BseMII CTCAG 2 cut(s) 30, 119
BseNI ACTGG 1 cut(s) 164
BseXI GCAGC 2 cut(s) 17, 133
BsgI GTGCAG 1 cut(s) 44
Bsh1236I CGCG 1 cut(s) 213
BshFI GGCC 1 cut(s) 210
BshNI GGYRCC 1 cut(s) 134
BsiHKAI GWGCWC 1 cut(s) 261
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 1 cut(s) 101
BslFI GGGAC 1 cut(s) 382
BsmFI GGGAC 1 cut(s) 382
BsnI GGCC 1 cut(s) 210
BsoBI CYCGRG 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 261
Bsp143I GATC 2 cut(s) 42, 385
Bsp1720I GCTNAGC 1 cut(s) 105
Bsp19I CCATGG 1 cut(s) 287
BspACI CCGC 6 cut(s) 79, 138, 174, 211, 242, 250
BspANI GGCC 1 cut(s) 210
BspCNI CTCAG 2 cut(s) 31, 118
BspFNI CGCG 1 cut(s) 213
BspLI GGNNCC 3 cut(s) 99, 136, 293
BspMI ACCTGC 1 cut(s) 93
BspPI GGATC 1 cut(s) 37
BspT107I GGYRCC 1 cut(s) 134
BsrI ACTGG 1 cut(s) 164
BssECI CCNNGG 1 cut(s) 287
BssMI GATC 2 cut(s) 42, 385
BssT1I CCWWGG 1 cut(s) 287
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 2 cut(s) 134, 263
BstC8I GCNNGC 1 cut(s) 96
BstDEI CTNAG 2 cut(s) 39, 105
BstDSI CCRYGG 1 cut(s) 287
BstF5I GGATG 1 cut(s) 49
BstFNI CGCG 1 cut(s) 213
BstHHI GCGC 1 cut(s) 249
BstKTI GATC 2 cut(s) 45, 388
BstMBI GATC 2 cut(s) 42, 385
BstMWI GCNNNNNNNGC 6 cut(s) 18, 27, 115, 143, 146, 207
BstNI CCWGG 1 cut(s) 111
BstSCI CCNGG 1 cut(s) 109
BstUI CGCG 1 cut(s) 213
BstV1I GCAGC 2 cut(s) 17, 133
BstX2I RGATCY 1 cut(s) 42
BstYI RGATCY 1 cut(s) 42
BsuRI GGCC 1 cut(s) 210
BtgI CCRYGG 1 cut(s) 287
BtsCI GGATG 1 cut(s) 49
BtsI GCAGTG 1 cut(s) 274
BtsIMutI CAGTG 2 cut(s) 36, 274
BveI ACCTGC 1 cut(s) 93
Cac8I GCNNGC 1 cut(s) 96
CfoI GCGC 1 cut(s) 249
Cfr13I GGNCC 1 cut(s) 208
CseI GACGC 1 cut(s) 13
Csp6I GTAC 1 cut(s) 312
CviAII CATG 3 cut(s) 197, 202, 288
CviJI RGCY 8 cut(s) 30, 94, 98, 104, 109, 210, 223, 450
CviKI_1 RGCY 8 cut(s) 30, 94, 98, 104, 109, 210, 223, 450
CviQI GTAC 1 cut(s) 312
DdeI CTNAG 2 cut(s) 39, 105
DpnI GATC 2 cut(s) 44, 387
DpnII GATC 2 cut(s) 42, 385
EciI GGCGGA 1 cut(s) 189
Eco130I CCWWGG 1 cut(s) 287
Eco88I CYCGRG 1 cut(s) 254
EcoRII CCWGG 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 287
ErhI CCWWGG 1 cut(s) 287
FaeI CATG 3 cut(s) 200, 205, 291
FaiI YATR 3 cut(s) 198, 203, 289
FaqI GGGAC 1 cut(s) 382
FatI CATG 3 cut(s) 196, 201, 287
FbaI TGATCA 1 cut(s) 385
Fnu4HI GCNGC 5 cut(s) 31, 138, 147, 211, 250
FokI GGATG 1 cut(s) 36
Fsp4HI GCNGC 5 cut(s) 31, 138, 147, 211, 250
FspBI CTAG 2 cut(s) 71, 302
GlaI GCGC 1 cut(s) 248
GluI GCNGC 5 cut(s) 31, 138, 147, 211, 250
GsuI CTGGAG 1 cut(s) 245
HaeIII GGCC 1 cut(s) 210
HapII CCGG 1 cut(s) 101
HgaI GACGC 1 cut(s) 13
HhaI GCGC 1 cut(s) 249
Hin1II CATG 3 cut(s) 200, 205, 291
Hin6I GCGC 1 cut(s) 247
HinP1I GCGC 1 cut(s) 247
HinfI GANTC 4 cut(s) 168, 177, 230, 298
HpaII CCGG 1 cut(s) 101
HphI GGTGA 1 cut(s) 68
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 2 cut(s) 47, 182
Hpy188III TCNNGA 2 cut(s) 155, 295
Hpy8I GTNNAC 1 cut(s) 76
HpyCH4III ACNGT 2 cut(s) 134, 263
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 2 cut(s) 61, 88
HpyF10VI GCNNNNNNNGC 6 cut(s) 18, 27, 115, 143, 146, 207
HpyF3I CTNAG 2 cut(s) 39, 105
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 3 cut(s) 200, 205, 291
HspAI GCGC 1 cut(s) 247
Ksp22I TGATCA 1 cut(s) 385
Kzo9I GATC 2 cut(s) 42, 385
LmnI GCTCC 3 cut(s) 38, 103, 322
Lsp1109I GCAGC 2 cut(s) 17, 133
LweI GCATC 1 cut(s) 133
MaeI CTAG 2 cut(s) 71, 302
MaeII ACGT 1 cut(s) 237
MalI GATC 2 cut(s) 44, 387
MboI GATC 2 cut(s) 42, 385
MboII GAAGA 3 cut(s) 269, 272, 422
MflI RGATCY 1 cut(s) 42
MhlI GDGCHC 1 cut(s) 261
MluCI AATT 1 cut(s) 356
MlyI GAGTC 3 cut(s) 177, 186, 292
MmeI TCCRAC 1 cut(s) 70
MspA1I CMGCKG 2 cut(s) 30, 94
MspI CCGG 1 cut(s) 101
MspR9I CCNGG 1 cut(s) 111
MvaI CCWGG 1 cut(s) 111
MvnI CGCG 1 cut(s) 213
MwoI GCNNNNNNNGC 6 cut(s) 18, 27, 115, 143, 146, 207
NcoI CCATGG 1 cut(s) 287
NdeII GATC 2 cut(s) 42, 385
NlaIII CATG 3 cut(s) 200, 205, 291
NlaIV GGNNCC 3 cut(s) 99, 136, 293
PaeR7I CTCGAG 1 cut(s) 254
PaqCI CACCTGC 1 cut(s) 93
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PfeI GAWTC 1 cut(s) 230
PkrI GCNGC 5 cut(s) 32, 139, 148, 212, 251
PleI GAGTC 3 cut(s) 176, 185, 292
PpsI GAGTC 3 cut(s) 176, 185, 292
Psp6I CCWGG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 109
PspN4I GGNNCC 3 cut(s) 99, 136, 293
PspPI GGNCC 1 cut(s) 208
PspXI VCTCGAGB 1 cut(s) 254
PsuI RGATCY 1 cut(s) 42
PvuII CAGCTG 2 cut(s) 30, 94
RsaI GTAC 1 cut(s) 313
RsaNI GTAC 1 cut(s) 312
SatI GCNGC 5 cut(s) 31, 138, 147, 211, 250
Sau3AI GATC 2 cut(s) 42, 385
Sau96I GGNCC 1 cut(s) 208
SchI GAGTC 3 cut(s) 177, 186, 292
ScrFI CCNGG 1 cut(s) 111
SduI GDGCHC 1 cut(s) 261
SetI ASST 6 cut(s) 32, 87, 96, 123, 240, 452
SfaNI GCATC 1 cut(s) 133
Sfr274I CTCGAG 1 cut(s) 254
SlaI CTCGAG 1 cut(s) 254
SmlI CTYRAG 2 cut(s) 217, 254
SmoI CTYRAG 2 cut(s) 217, 254
SpeI ACTAGT 1 cut(s) 70
Sse9I AATT 1 cut(s) 356
SsiI CCGC 6 cut(s) 79, 138, 174, 211, 242, 250
SspI AATATT 1 cut(s) 440
SspMI CTAG 2 cut(s) 71, 302
StyD4I CCNGG 1 cut(s) 109
StyI CCWWGG 1 cut(s) 287
TaaI ACNGT 2 cut(s) 134, 263
TaiI ACGT 1 cut(s) 240
TaqI TCGA 2 cut(s) 233, 255
TasI AATT 1 cut(s) 356
TauI GCSGC 3 cut(s) 140, 213, 252
TfiI GAWTC 1 cut(s) 230
TscAI CASTG 2 cut(s) 43, 274
TseI GCWGC 2 cut(s) 30, 146
TspGWI ACGGA 1 cut(s) 386
TspRI CASTG 2 cut(s) 43, 274
XhoI CTCGAG 1 cut(s) 254
XspI CTAG 2 cut(s) 71, 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.