RLG00000003754
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0018732
Physical Location & Seq
Forward (+)
52304499 .. 52305500
1002 bp
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UTR
Exon/CDS
Intron
RLM00000003754

Sequence Viewer

Length: 420 bp
ATGGCAGCACGTCTTCACTCCACTCGTGCTCTAGGAACCTTTGGTTATCATGCCCCAGAATATGTGATGACTGGACAGTTGAATGCCAAAAGTGATACTAACACTTTTGGTGTTGTCCTGTTAGAGCTTTTGACTGGAAGAAAACCTGTTGATCATACATTACTACGGGGACAGCAGAGCCTGGTGACATGGGTACTATATCTGCTATCTGTCTCTTATGAAGGCAAAGTTAGGTGGTGCATTGATGCAAGACTAGGAGGAGAATTCCTCACCAAGGCTGTTGCAAAGATGGCTGCAGTTGCTGTCTTGTGTGTGCAATATGAGGCTGATTTCCGCCCAAACATGAGCATTGTGATCAAAGCCCTACAACCACTGCTAAATGCTCGGCCTGGACCCACTGGTGAATCGCCAAGCTTGTGA

Protein Analysis

140

Amino Acids

15.19

Weight (kDa)

9.26

Isoelectric Point (pI)

29.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 14 - 121 4.4e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018732)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62220
pyrus_communis pycom13g12270 pycom16g12110
rosa_chinensis RchiOBHm_Chr3g0487491
rosa_laevigata RLG00000003754 RLG00000023002
rosa_multiflora Rmu_co8428993.1_g000001 Rmu_sc0009883.1_g000004
rosa_samantha Rh1CG082500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 334
AcsI RAATTY 1 cut(s) 263
AfaI GTAC 1 cut(s) 195
AfiI CCNNNNNNNGG 1 cut(s) 274
AgsI TTSAA 1 cut(s) 82
AjiI CACGTC 1 cut(s) 11
AjnI CCWGG 2 cut(s) 180, 388
AluBI AGCT 2 cut(s) 127, 414
AluI AGCT 2 cut(s) 127, 414
Alw21I GWGCWC 1 cut(s) 31
Alw26I GTCTC 1 cut(s) 217
AlwNI CAGNNNCTG 2 cut(s) 181, 302
AoxI GGCC 1 cut(s) 386
ApeKI GCWGC 2 cut(s) 5, 293
ApoI RAATTY 1 cut(s) 263
AspS9I GGNCC 1 cut(s) 392
AsuHPI GGTGA 3 cut(s) 196, 262, 413
AvaII GGWCC 1 cut(s) 392
BauI CACGAG 1 cut(s) 24
BbsI GAAGAC 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 31
BbvI GCAGC 2 cut(s) 17, 280
BccI CCATC 1 cut(s) 283
BciT130I CCWGG 2 cut(s) 182, 390
BclI TGATCA 2 cut(s) 151, 354
BcoDI GTCTC 1 cut(s) 217
BfaI CTAG 2 cut(s) 32, 254
BfmI CTRYAG 1 cut(s) 294
BisI GCNGC 2 cut(s) 6, 294
BlsI GCNGC 2 cut(s) 7, 295
Bme1390I CCNGG 2 cut(s) 182, 390
Bme18I GGWCC 1 cut(s) 392
BmgBI CACGTC 1 cut(s) 11
BmgT120I GGNCC 1 cut(s) 392
BmiI GGNNCC 2 cut(s) 37, 394
BmrFI CCNGG 2 cut(s) 182, 390
BmsI GCATC 1 cut(s) 235
BpiI GAAGAC 1 cut(s) 5
BplI GAGNNNNNCTC 2 cut(s) 252, 284
BsaJI CCNNGG 1 cut(s) 273
Bsc4I CCNNNNNNNGG 1 cut(s) 274
Bse1I ACTGG 3 cut(s) 76, 139, 403
BseBI CCWGG 2 cut(s) 182, 390
BseDI CCNNGG 1 cut(s) 273
BseLI CCNNNNNNNGG 1 cut(s) 274
BseNI ACTGG 3 cut(s) 76, 139, 403
BseRI GAGGAG 1 cut(s) 273
BseXI GCAGC 2 cut(s) 17, 280
BshFI GGCC 1 cut(s) 388
BsiHKAI GWGCWC 1 cut(s) 31
BslFI GGGAC 1 cut(s) 183
BslI CCNNNNNNNGG 1 cut(s) 274
BsmAI GTCTC 1 cut(s) 217
BsmFI GGGAC 1 cut(s) 183
BsmI GAATGC 1 cut(s) 88
BsnI GGCC 1 cut(s) 388
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 2 cut(s) 151, 354
BspACI CCGC 1 cut(s) 334
BspANI GGCC 1 cut(s) 388
BspLI GGNNCC 2 cut(s) 37, 394
BspMAI CTGCAG 1 cut(s) 298
BsrI ACTGG 3 cut(s) 76, 139, 403
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 2 cut(s) 151, 354
BssSI CACGAG 1 cut(s) 24
BssT1I CCWWGG 1 cut(s) 273
Bst2BI CACGAG 1 cut(s) 24
Bst2UI CCWGG 2 cut(s) 182, 390
Bst4CI ACNGT 1 cut(s) 78
BstENI CCTNNNNNAGG 1 cut(s) 272
BstKTI GATC 2 cut(s) 154, 357
BstMAI GTCTC 1 cut(s) 217
BstMBI GATC 2 cut(s) 151, 354
BstMWI GCNNNNNNNGC 2 cut(s) 290, 299
BstNI CCWGG 2 cut(s) 182, 390
BstSCI CCNGG 2 cut(s) 180, 388
BstSFI CTRYAG 1 cut(s) 294
BstV1I GCAGC 2 cut(s) 17, 280
BstV2I GAAGAC 1 cut(s) 5
BsuRI GGCC 1 cut(s) 388
BtrI CACGTC 1 cut(s) 11
BtsI GCAGTG 1 cut(s) 371
BtsIMutI CAGTG 2 cut(s) 371, 396
CaiI CAGNNNCTG 2 cut(s) 181, 302
Cfr13I GGNCC 1 cut(s) 392
Csp6I GTAC 1 cut(s) 194
CviAII CATG 3 cut(s) 50, 189, 343
CviJI RGCY 8 cut(s) 127, 180, 278, 293, 326, 362, 388, 414
CviKI_1 RGCY 8 cut(s) 127, 180, 278, 293, 326, 362, 388, 414
CviQI GTAC 1 cut(s) 194
DpnI GATC 2 cut(s) 153, 356
DpnII GATC 2 cut(s) 151, 354
EciI GGCGGA 1 cut(s) 323
Eco130I CCWWGG 1 cut(s) 273
Eco47I GGWCC 1 cut(s) 392
EcoNI CCTNNNNNAGG 1 cut(s) 272
EcoRI GAATTC 1 cut(s) 263
EcoRII CCWGG 2 cut(s) 180, 388
EcoT14I CCWWGG 1 cut(s) 273
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 3 cut(s) 53, 192, 346
FaiI YATR 8 cut(s) 51, 63, 156, 190, 199, 219, 321, 344
FaqI GGGAC 1 cut(s) 183
FatI CATG 3 cut(s) 49, 188, 342
FbaI TGATCA 2 cut(s) 151, 354
Fnu4HI GCNGC 2 cut(s) 6, 294
Fsp4HI GCNGC 2 cut(s) 6, 294
FspBI CTAG 2 cut(s) 32, 254
GluI GCNGC 2 cut(s) 6, 294
HaeIII GGCC 1 cut(s) 388
Hin1II CATG 3 cut(s) 53, 192, 346
HindIII AAGCTT 1 cut(s) 412
HinfI GANTC 1 cut(s) 404
HphI GGTGA 3 cut(s) 196, 262, 413
HpyAV CCTTC 1 cut(s) 215
HpyCH4III ACNGT 1 cut(s) 78
HpyCH4IV ACGT 1 cut(s) 10
HpyCH4V TGCA 5 cut(s) 240, 248, 284, 296, 316
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 299
HpySE526I ACGT 1 cut(s) 10
Hsp92II CATG 3 cut(s) 53, 192, 346
Ksp22I TGATCA 2 cut(s) 151, 354
Kzo9I GATC 2 cut(s) 151, 354
Lsp1109I GCAGC 2 cut(s) 17, 280
LweI GCATC 1 cut(s) 235
MaeI CTAG 2 cut(s) 32, 254
MaeII ACGT 1 cut(s) 10
MaeIII GTNAC 1 cut(s) 184
MalI GATC 2 cut(s) 153, 356
MboI GATC 2 cut(s) 151, 354
MboII GAAGA 2 cut(s) 5, 150
MhlI GDGCHC 1 cut(s) 31
MluCI AATT 1 cut(s) 263
MnlI CCTC 3 cut(s) 251, 278, 316
MspR9I CCNGG 2 cut(s) 182, 390
Mva1269I GAATGC 1 cut(s) 88
MvaI CCWGG 2 cut(s) 182, 390
MwoI GCNNNNNNNGC 2 cut(s) 290, 299
NdeII GATC 2 cut(s) 151, 354
NlaIII CATG 3 cut(s) 53, 192, 346
NlaIV GGNNCC 2 cut(s) 37, 394
NmeAIII GCCGAG 1 cut(s) 364
NmuCI GTSAC 1 cut(s) 184
PctI GAATGC 1 cut(s) 88
PfeI GAWTC 1 cut(s) 404
PkrI GCNGC 2 cut(s) 7, 295
Psp6I CCWGG 2 cut(s) 180, 388
PspGI CCWGG 2 cut(s) 180, 388
PspN4I GGNNCC 2 cut(s) 37, 394
PspPI GGNCC 1 cut(s) 392
PstI CTGCAG 1 cut(s) 298
PstNI CAGNNNCTG 2 cut(s) 181, 302
RsaI GTAC 1 cut(s) 195
RsaNI GTAC 1 cut(s) 194
SatI GCNGC 2 cut(s) 6, 294
Sau3AI GATC 2 cut(s) 151, 354
Sau96I GGNCC 1 cut(s) 392
ScrFI CCNGG 2 cut(s) 182, 390
SduI GDGCHC 1 cut(s) 31
SetI ASST 6 cut(s) 13, 41, 129, 148, 236, 416
SfaNI GCATC 1 cut(s) 235
SfcI CTRYAG 1 cut(s) 294
SinI GGWCC 1 cut(s) 392
Sse9I AATT 1 cut(s) 263
SsiI CCGC 1 cut(s) 334
SspMI CTAG 2 cut(s) 32, 254
StyD4I CCNGG 2 cut(s) 180, 388
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 1 cut(s) 78
TaiI ACGT 1 cut(s) 13
TasI AATT 1 cut(s) 263
TfiI GAWTC 1 cut(s) 404
TscAI CASTG 2 cut(s) 378, 403
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 2 cut(s) 5, 293
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 1 cut(s) 234
TspRI CASTG 2 cut(s) 378, 403
VpaK11BI GGWCC 1 cut(s) 392
XagI CCTNNNNNAGG 1 cut(s) 272
XapI RAATTY 1 cut(s) 263
XspI CTAG 2 cut(s) 32, 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.