RLG00000004728

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0018210
Physical Location & Seq
Forward (+)
61771051 .. 61772611
1561 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004728

Sequence Viewer

Length: 1140 bp
ATGGAGTACCTCCCGCAAGACCTAATATATGAAATTGTGTTACGACTCCCTGTGAAGGGTCTGATCCGATTTTCTTTTGTTTCGAAGCTATGGAAGGCTGTGATCTACAGCAAAGACTTCATTAAAGAGCATCAGCAACGCCGCTCTATCAACAACTCCAAGAAAGATTTCACCCTCATACGCCATGGTGTTCAGTCCGGAGATTTTCATCACTTCTATGCAGCCTCCTTTTCTGCTGATACATTAGAGGAAGAACAACAAATCGAGCATCCAATATATTCCCTTGAAGAAGAGTATGGACTTGGGCTTCATACTCGTATCATTACTGTCTGCCATGGATTGGTTTTACTAGGTGTTCTGTATTATAGGAAGGCTACTAGTAATTATATGCACCTCTGTGTATGGAACCCGGTGATCGCAAAGCACAGATACATATCCCAGCCCATATGGCCAGAAGGAGAAGACCCGCATTTTACGTATGGAATAGGTTATCATTGTGACGAAGATGACTTAAAGGTGTTAGTTTTGAGGCAAACTGGTAGATGGCCGATTTGGAAAATCCAGTTTATAGTTTGCAGCGTAGCAACAGGCCAATGGAAAAGGATAACAAAACTTCCACCTAGTAATCTTCAAGTTGATATATTTTGTTATTATAATATGGTGTCGTTGAGCGATTCTCGTGTGGCTTGGCTGATGAGAGATGTTGATTATATGGATCTGTACCATGTTGTGACTTTTGACCTTGTGAAGGAAGAGTATAATACTTATCGGGTTCCGATTTCTATTCGTGGTCCAGGGGCTATGACGCTGATGGAACTGGGAGGCTTCCTGTGCTTTAGGCATGGTCAAAATATTTGGTTCATGAAGGATTCTTCTTGGTCGACCGAGCCTATTCCTCTGAATAATTCCAGGCCTCTATTCTTTGATGGTGAGAGGTTTCTGTTGAGCAGGTTTTCTGGTGATCATAAGGAGCTCTTTTGGTACAACATAAAGACACAAGATCATCAACATGTGACGCGAACTGGTGACTATGCTGGAAGCTTTGATGGGGCTACTACTCAGACAGCTTTCATTTGTGGAGGAAACCTCCGTTTTCTCCTCGATGGTCAACCTGATCTGTTGATAGAGGCACCGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1

Protein Analysis

380

Amino Acids

44.21

Weight (kDa)

6.34

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 4 - 41 1.2e-07 F-box domain
FBA_1 PF07734 72 - 261 4.2e-08 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018210)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0184151 RchiOBHm_Chr7g0188081
rosa_laevigata RLG00000004728
rosa_multiflora Rmu_co8443907.1_g000001
rosa_rugosa Rorug06G0463300 Rorug06G0488200
rosa_samantha Rh7BG064500 Rh7CG093300 Rh7DG064800 Rh7DG094300
rosa_wichuraiana Rw0G002520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 654
Acc36I ACCTGC 1 cut(s) 939
AccB1I GGYRCC 1 cut(s) 1129
AccB7I CCANNNNNTGG 1 cut(s) 340
AccBSI CCGCTC 1 cut(s) 144
AccI GTMKAC 1 cut(s) 881
AccII CGCG 1 cut(s) 1018
AccIII TCCGGA 1 cut(s) 197
AciI CCGC 3 cut(s) 14, 142, 467
AclWI GGATC 2 cut(s) 58, 723
AcoI YGGCCR 2 cut(s) 449, 545
AfaI GTAC 3 cut(s) 8, 722, 983
AfiI CCNNNNNNNGG 4 cut(s) 55, 56, 340, 748
AflIII ACRYGT 1 cut(s) 1009
AgsI TTSAA 2 cut(s) 287, 632
AhlI ACTAGT 1 cut(s) 377
AjnI CCWGG 2 cut(s) 793, 908
AjuI GAANNNNNNNTTGG 2 cut(s) 152, 184
AleI CACNNNNGTG 1 cut(s) 396
AluBI AGCT 4 cut(s) 88, 973, 1041, 1067
AluI AGCT 4 cut(s) 88, 973, 1041, 1067
Alw21I GWGCWC 1 cut(s) 975
AlwI GGATC 2 cut(s) 58, 723
Aor13HI TCCGGA 1 cut(s) 197
AoxI GGCC 4 cut(s) 449, 545, 589, 911
ApeKI GCWGC 2 cut(s) 221, 576
Asp700I GAANNNNTTC 1 cut(s) 167
AspS9I GGNCC 1 cut(s) 791
AsuC2I CCSGG 1 cut(s) 410
AsuHPI GGTGA 5 cut(s) 163, 424, 941, 971, 1037
AsuII TTCGAA 1 cut(s) 83
AvaII GGWCC 1 cut(s) 791
BalI TGGCCA 1 cut(s) 451
BanI GGYRCC 1 cut(s) 1129
BanII GRGCYC 1 cut(s) 975
BauI CACGAG 1 cut(s) 678
BbsI GAAGAC 1 cut(s) 468
Bbv12I GWGCWC 1 cut(s) 975
BbvI GCAGC 2 cut(s) 233, 588
BccI CCATC 5 cut(s) 537, 805, 920, 1040, 1097
BciT130I CCWGG 2 cut(s) 795, 910
BclI TGATCA 1 cut(s) 961
BcnI CCSGG 1 cut(s) 410
BcuI ACTAGT 1 cut(s) 377
BfaI CTAG 3 cut(s) 350, 378, 621
BfmI CTRYAG 1 cut(s) 106
BfuAI ACCTGC 1 cut(s) 939
BglI GCCNNNNNGGC 1 cut(s) 448
BisI GCNGC 3 cut(s) 142, 222, 577
BlsI GCNGC 3 cut(s) 143, 223, 578
Bme1390I CCNGG 3 cut(s) 410, 795, 910
Bme18I GGWCC 1 cut(s) 791
BmgT120I GGNCC 1 cut(s) 791
BmiI GGNNCC 3 cut(s) 407, 774, 1131
BmrFI CCNGG 3 cut(s) 410, 795, 910
BmrI ACTGGG 1 cut(s) 827
BmsI GCATC 2 cut(s) 139, 277
BmuI ACTGGG 1 cut(s) 827
BpiI GAAGAC 1 cut(s) 468
BplI GAGNNNNNCTC 4 cut(s) 661, 693, 1071, 1103
Bpu14I TTCGAA 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 410
BsaAI YACGTR 1 cut(s) 477
BsaBI GATNNNNATC 2 cut(s) 207, 433
BsaJI CCNNGG 3 cut(s) 184, 334, 794
BsaWI WCCGGW 1 cut(s) 197
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 56, 340, 748
Bse1I ACTGG 4 cut(s) 541, 562, 822, 1027
Bse8I GATNNNNATC 2 cut(s) 207, 433
BseAI TCCGGA 1 cut(s) 197
BseBI CCWGG 2 cut(s) 795, 910
BseDI CCNNGG 3 cut(s) 184, 334, 794
BseGI GGATG 1 cut(s) 268
BseJI GATNNNNATC 2 cut(s) 207, 433
BseLI CCNNNNNNNGG 4 cut(s) 55, 56, 340, 748
BseMII CTCAG 1 cut(s) 1073
BseNI ACTGG 4 cut(s) 541, 562, 822, 1027
BseRI GAGGAG 1 cut(s) 1088
BseXI GCAGC 2 cut(s) 233, 588
BseYI CCCAGC 1 cut(s) 438
Bsh1236I CGCG 1 cut(s) 1018
Bsh1285I CGRYCG 1 cut(s) 885
BshFI GGCC 4 cut(s) 451, 547, 591, 913
BshNI GGYRCC 1 cut(s) 1129
BsiEI CGRYCG 1 cut(s) 885
BsiHKAI GWGCWC 1 cut(s) 975
BsiSI CCGG 2 cut(s) 198, 410
BslI CCNNNNNNNGG 4 cut(s) 55, 56, 340, 748
BsnI GGCC 4 cut(s) 451, 547, 591, 913
Bsp119I TTCGAA 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 975
Bsp13I TCCGGA 1 cut(s) 197
Bsp143I GATC 7 cut(s) 63, 102, 414, 715, 961, 1000, 1114
Bsp19I CCATGG 2 cut(s) 184, 334
BspACI CCGC 3 cut(s) 14, 142, 467
BspANI GGCC 4 cut(s) 451, 547, 591, 913
BspCNI CTCAG 1 cut(s) 1072
BspEI TCCGGA 1 cut(s) 197
BspFNI CGCG 1 cut(s) 1018
BspHI TCATGA 1 cut(s) 861
BspLI GGNNCC 3 cut(s) 407, 774, 1131
BspMI ACCTGC 1 cut(s) 939
BspPI GGATC 2 cut(s) 58, 723
BspT104I TTCGAA 1 cut(s) 83
BspT107I GGYRCC 1 cut(s) 1129
BsrBI CCGCTC 1 cut(s) 144
BsrI ACTGG 4 cut(s) 541, 562, 822, 1027
BssECI CCNNGG 3 cut(s) 184, 334, 794
BssMI GATC 7 cut(s) 63, 102, 414, 715, 961, 1000, 1114
BssSI CACGAG 1 cut(s) 678
BssT1I CCWWGG 2 cut(s) 184, 334
Bst2BI CACGAG 1 cut(s) 678
Bst2UI CCWGG 2 cut(s) 795, 910
Bst4CI ACNGT 2 cut(s) 328, 1134
Bst6I CTCTTC 2 cut(s) 285, 747
BstBAI YACGTR 1 cut(s) 477
BstBI TTCGAA 1 cut(s) 83
BstDEI CTNAG 1 cut(s) 1059
BstDSI CCRYGG 2 cut(s) 184, 334
BstENI CCTNNNNNAGG 1 cut(s) 746
BstF5I GGATG 1 cut(s) 268
BstFNI CGCG 1 cut(s) 1018
BstKTI GATC 7 cut(s) 66, 105, 417, 718, 964, 1003, 1117
BstMBI GATC 7 cut(s) 63, 102, 414, 715, 961, 1000, 1114
BstMCI CGRYCG 1 cut(s) 885
BstMWI GCNNNNNNNGC 2 cut(s) 448, 831
BstNI CCWGG 2 cut(s) 795, 910
BstNSI RCATGY 1 cut(s) 1013
BstSCI CCNGG 3 cut(s) 408, 793, 908
BstSFI CTRYAG 1 cut(s) 106
BstSNI TACGTA 1 cut(s) 477
BstUI CGCG 1 cut(s) 1018
BstV1I GCAGC 2 cut(s) 233, 588
BstV2I GAAGAC 1 cut(s) 468
BstX2I RGATCY 1 cut(s) 715
BstYI RGATCY 1 cut(s) 715
BsuRI GGCC 4 cut(s) 451, 547, 591, 913
BtgI CCRYGG 2 cut(s) 184, 334
BtsCI GGATG 1 cut(s) 268
BveI ACCTGC 1 cut(s) 939
CciI TCATGA 1 cut(s) 861
Cfr13I GGNCC 1 cut(s) 791
CseI GACGC 2 cut(s) 814, 1024
Csp6I GTAC 3 cut(s) 7, 721, 982
CviAII CATG 6 cut(s) 185, 335, 725, 842, 862, 1010
CviQI GTAC 3 cut(s) 7, 721, 982
DdeI CTNAG 1 cut(s) 1059
DpnI GATC 7 cut(s) 65, 104, 416, 717, 963, 1002, 1116
DpnII GATC 7 cut(s) 63, 102, 414, 715, 961, 1000, 1114
EaeI YGGCCR 2 cut(s) 449, 545
Eam1104I CTCTTC 2 cut(s) 285, 747
EarI CTCTTC 2 cut(s) 285, 747
Ecl136II GAGCTC 1 cut(s) 973
Eco105I TACGTA 1 cut(s) 477
Eco130I CCWWGG 2 cut(s) 184, 334
Eco147I AGGCCT 1 cut(s) 913
Eco24I GRGCYC 1 cut(s) 975
Eco47I GGWCC 1 cut(s) 791
Eco53kI GAGCTC 1 cut(s) 973
EcoICRI GAGCTC 1 cut(s) 973
EcoNI CCTNNNNNAGG 1 cut(s) 746
EcoRII CCWGG 2 cut(s) 793, 908
EcoT14I CCWWGG 2 cut(s) 184, 334
EcoT38I GRGCYC 1 cut(s) 975
ErhI CCWWGG 2 cut(s) 184, 334
FaeI CATG 6 cut(s) 188, 338, 728, 845, 865, 1013
FalI AAGNNNNNCTT 2 cut(s) 959, 991
FatI CATG 6 cut(s) 184, 334, 724, 841, 861, 1009
FauI CCCGC 2 cut(s) 21, 474
FauNDI CATATG 1 cut(s) 446
FbaI TGATCA 1 cut(s) 961
FblI GTMKAC 1 cut(s) 881
Fnu4HI GCNGC 3 cut(s) 142, 222, 577
FokI GGATG 1 cut(s) 255
FriOI GRGCYC 1 cut(s) 975
Fsp4HI GCNGC 3 cut(s) 142, 222, 577
FspBI CTAG 3 cut(s) 350, 378, 621
GluI GCNGC 3 cut(s) 142, 222, 577
GsaI CCCAGC 1 cut(s) 442
HaeIII GGCC 4 cut(s) 451, 547, 591, 913
HapII CCGG 2 cut(s) 198, 410
HgaI GACGC 2 cut(s) 814, 1024
Hin1II CATG 6 cut(s) 188, 338, 728, 845, 865, 1013
HincII GTYRAC 2 cut(s) 882, 1109
HindII GTYRAC 2 cut(s) 882, 1109
HindIII AAGCTT 1 cut(s) 1039
HinfI GANTC 3 cut(s) 45, 674, 869
HpaII CCGG 2 cut(s) 198, 410
HphI GGTGA 5 cut(s) 163, 424, 941, 971, 1037
Hpy166II GTNNAC 2 cut(s) 882, 1109
Hpy188I TCNGA 5 cut(s) 63, 68, 777, 900, 1062
Hpy188III TCNNGA 2 cut(s) 198, 862
Hpy8I GTNNAC 2 cut(s) 882, 1109
HpyAV CCTTC 6 cut(s) 49, 88, 364, 449, 742, 859
HpyCH4III ACNGT 2 cut(s) 328, 1134
HpyCH4IV ACGT 1 cut(s) 476
HpyCH4V TGCA 3 cut(s) 221, 391, 576
HpyF10VI GCNNNNNNNGC 2 cut(s) 448, 831
HpyF3I CTNAG 1 cut(s) 1059
HpySE526I ACGT 1 cut(s) 476
Hsp92II CATG 6 cut(s) 188, 338, 728, 845, 865, 1013
Kpn2I TCCGGA 1 cut(s) 197
Ksp22I TGATCA 1 cut(s) 961
Kzo9I GATC 7 cut(s) 63, 102, 414, 715, 961, 1000, 1114
LmnI GCTCC 1 cut(s) 970
Lsp1109I GCAGC 2 cut(s) 233, 588
LweI GCATC 2 cut(s) 139, 277
MaeI CTAG 3 cut(s) 350, 378, 621
MaeII ACGT 1 cut(s) 476
MaeIII GTNAC 5 cut(s) 39, 497, 730, 1012, 1025
MalI GATC 7 cut(s) 65, 104, 416, 717, 963, 1002, 1116
MbiI CCGCTC 1 cut(s) 144
MboI GATC 7 cut(s) 63, 102, 414, 715, 961, 1000, 1114
MboII GAAGA 8 cut(s) 263, 299, 302, 473, 515, 620, 764, 864
MflI RGATCY 1 cut(s) 715
MhlI GDGCHC 1 cut(s) 975
MlsI TGGCCA 1 cut(s) 451
MluCI AATT 3 cut(s) 33, 382, 904
MluNI TGGCCA 1 cut(s) 451
MlyI GAGTC 1 cut(s) 39
Mox20I TGGCCA 1 cut(s) 451
MroI TCCGGA 1 cut(s) 197
MroXI GAANNNNTTC 1 cut(s) 167
MscI TGGCCA 1 cut(s) 451
MseI TTAA 2 cut(s) 123, 512
MslI CAYNNNNRTG 3 cut(s) 216, 396, 1008
Msp20I TGGCCA 1 cut(s) 451
MspI CCGG 2 cut(s) 198, 410
MspR9I CCNGG 3 cut(s) 410, 795, 910
MvaI CCWGG 2 cut(s) 795, 910
MvnI CGCG 1 cut(s) 1018
MwoI GCNNNNNNNGC 2 cut(s) 448, 831
NciI CCSGG 1 cut(s) 410
NcoI CCATGG 2 cut(s) 184, 334
NdeI CATATG 1 cut(s) 446
NdeII GATC 7 cut(s) 63, 102, 414, 715, 961, 1000, 1114
NlaIII CATG 6 cut(s) 188, 338, 728, 845, 865, 1013
NlaIV GGNNCC 3 cut(s) 407, 774, 1131
NmuCI GTSAC 4 cut(s) 497, 730, 1012, 1025
NspI RCATGY 1 cut(s) 1013
NspV TTCGAA 1 cut(s) 83
OliI CACNNNNGTG 1 cut(s) 396
PagI TCATGA 1 cut(s) 861
PceI AGGCCT 1 cut(s) 913
PciI ACATGT 1 cut(s) 1009
PdmI GAANNNNTTC 1 cut(s) 167
PfeI GAWTC 2 cut(s) 674, 869
PflMI CCANNNNNTGG 1 cut(s) 340
PkrI GCNGC 3 cut(s) 143, 223, 578
PleI GAGTC 1 cut(s) 39
PpsI GAGTC 1 cut(s) 39
Ppu21I YACGTR 1 cut(s) 477
PscI ACATGT 1 cut(s) 1009
PsiI TTATAA 1 cut(s) 654
Psp124BI GAGCTC 1 cut(s) 975
Psp6I CCWGG 2 cut(s) 793, 908
PspFI CCCAGC 1 cut(s) 438
PspGI CCWGG 2 cut(s) 793, 908
PspN4I GGNNCC 3 cut(s) 407, 774, 1131
PspPI GGNCC 1 cut(s) 791
PsuI RGATCY 1 cut(s) 715
RsaI GTAC 3 cut(s) 8, 722, 983
RsaNI GTAC 3 cut(s) 7, 721, 982
RseI CAYNNNNRTG 3 cut(s) 216, 396, 1008
SacI GAGCTC 1 cut(s) 975
SalI GTCGAC 1 cut(s) 880
SaqAI TTAA 2 cut(s) 123, 512
SatI GCNGC 3 cut(s) 142, 222, 577
Sau3AI GATC 7 cut(s) 63, 102, 414, 715, 961, 1000, 1114
Sau96I GGNCC 1 cut(s) 791
SchI GAGTC 1 cut(s) 39
ScrFI CCNGG 3 cut(s) 410, 795, 910
SduI GDGCHC 1 cut(s) 975
SfaNI GCATC 2 cut(s) 139, 277
SfcI CTRYAG 1 cut(s) 106
SfuI TTCGAA 1 cut(s) 83
SinI GGWCC 1 cut(s) 791
SmiMI CAYNNNNRTG 3 cut(s) 216, 396, 1008
SnaBI TACGTA 1 cut(s) 477
SpeI ACTAGT 1 cut(s) 377
Sse9I AATT 3 cut(s) 33, 382, 904
SseBI AGGCCT 1 cut(s) 913
SsiI CCGC 3 cut(s) 14, 142, 467
SspI AATATT 1 cut(s) 853
SspMI CTAG 3 cut(s) 350, 378, 621
SstI GAGCTC 1 cut(s) 975
StuI AGGCCT 1 cut(s) 913
StyD4I CCNGG 3 cut(s) 408, 793, 908
StyI CCWWGG 2 cut(s) 184, 334
TaaI ACNGT 2 cut(s) 328, 1134
TaiI ACGT 1 cut(s) 479
TaqI TCGA 4 cut(s) 83, 264, 881, 1101
TaqII GACCGA 1 cut(s) 899
TasI AATT 3 cut(s) 33, 382, 904
TauI GCSGC 1 cut(s) 144
TfiI GAWTC 2 cut(s) 674, 869
Tru1I TTAA 2 cut(s) 123, 512
Tru9I TTAA 2 cut(s) 123, 512
TseFI GTSAC 4 cut(s) 497, 730, 1012, 1025
TseI GCWGC 2 cut(s) 221, 576
Tsp45I GTSAC 4 cut(s) 497, 730, 1012, 1025
TspDTI ATGAA 7 cut(s) 45, 109, 197, 299, 850, 878, 1060
TspGWI ACGGA 1 cut(s) 1079
Van91I CCANNNNNTGG 1 cut(s) 340
VpaK11BI GGWCC 1 cut(s) 791
XagI CCTNNNNNAGG 1 cut(s) 746
XceI RCATGY 1 cut(s) 1013
XmiI GTMKAC 1 cut(s) 881
XmnI GAANNNNTTC 1 cut(s) 167
XspI CTAG 3 cut(s) 350, 378, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.