Rw0G002520

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00074
OG0018210
Physical Location & Seq
Forward (+)
30129 .. 31325
1197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G002520.1

Sequence Viewer

Length: 1197 bp
ATGTCAGAAGCTCTCAAACAGTCGTCAATGGAGTACCTCCCGCAGGACCTAATACATGAAATTGTTTTAAGACTCCCTGTTAAGTGTCTAATCCGATTTTCTTTCGTTTCAAAGCTATGGAAGGCTGTCATCCACAGCAAAGACTTCATTCGAAAGCATCGTCAACGCTTTAATCTCAACCACTTAAATGATTTTGCCCTCATTCGCCATCGTGTTGAATCTGATCCTCATGATCACTTCTATTCAGTAACCTTTTCTGGTGATGATACCACGTTAGAAGAAGAACGATTGAAGCATCCATTATATTCCCTCGAAGAAAGTGATAGACTTGGATCTGGCATCGTTGCCCTTTGCAATGGATTGATTTTACTTCATGTTAGAAGTAAGAAATATGATCTTGGTCTCTTTTTATGGAACCCCATGATTTCAAAGCATAAAATTATACCCACCCCCTTCAAATGGGCACGAGGACAATTCTCGAATCGGATGTACGGATTGGGCTATCATTCTGCTGAAGATGACTTGAAGGTGTTGGTTCTTACCGAAGACCATGATAATAGGTCGAATTCTCTTTTTGGACAAATAAATTTGACGATGGATTTAAAAGTGTACAGCGTAGCATTAAACAGATGGAAAAGGATAGAAAAGCTTCCACCCAGTAATCTTCGAATTTATGTAAATCATCAATCGAATATGGTGTCAATGAACGATTCTCGTGTAGCTTGGCTGATGAGAGACAATGATCAATTAGATCTGTACATGGTTGTGACTTTTGATCTTGTGAAGGAGGAGTATCAGTTTTTTCGTGTTCCGATTCTTATTCGGGGTCCGGGAGTAGTAAAACTGATGGCATTCGGAGGCTTCTTATGCTTTTTGCGCTGTTATACAGGTGACTGTATAAATGTTTGGTTTACAAAAGATTGCGATTCTTGCTGGTCGTTGGAGCCTGTTGTGTTGAATTGCCAGCCTTTATTCTTTGATGGTGAGAGGTTTCTCTTGAACCAGTTGAACCATGATGGCAATGATAAACTGATTTGGTACAATATAAAGACACAAAAACATGATTATGGGACAGAAATTCGTCGGCATACTCGCAGCAATCGGTGTACAGAAACAACTCTATGTGTGGGAGACCTCAGTCTTCTACTTGATGCTGATCCTGATGGTCGTCGTAGCTACTTTGGCCATGTATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

398

Amino Acids

46.63

Weight (kDa)

8.0

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 13 - 51 2.1e-09 F-box domain
FBA_3 PF08268 107 - 340 1.4e-11 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018210)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0184151 RchiOBHm_Chr7g0188081
rosa_laevigata RLG00000004728
rosa_multiflora Rmu_co8443907.1_g000001
rosa_rugosa Rorug06G0463300 Rorug06G0488200
rosa_samantha Rh7BG064500 Rh7CG093300 Rh7DG064800 Rh7DG094300
rosa_wichuraiana Rw0G002520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 41
AclWI GGATC 3 cut(s) 218, 340, 1151
AcoI YGGCCR 1 cut(s) 1183
AcsI RAATTY 4 cut(s) 565, 586, 669, 1077
AcuI CTGAAG 1 cut(s) 534
AfaI GTAC 6 cut(s) 35, 491, 611, 758, 1040, 1108
AfiI CCNNNNNNNGG 2 cut(s) 43, 459
AgsI TTSAA 9 cut(s) 111, 218, 292, 429, 457, 526, 958, 1000, 1009
AluBI AGCT 5 cut(s) 11, 115, 649, 722, 1176
AluI AGCT 5 cut(s) 11, 115, 649, 722, 1176
Alw26I GTCTC 3 cut(s) 407, 729, 1125
AlwI GGATC 3 cut(s) 218, 340, 1151
AoxI GGCC 1 cut(s) 1183
ApeKI GCWGC 1 cut(s) 1095
ApoI RAATTY 4 cut(s) 565, 586, 669, 1077
ArsI GACNNNNNNTTYG 4 cut(s) 145, 177, 683, 715
Asp700I GAANNNNTTC 1 cut(s) 648
AspLEI GCGC 1 cut(s) 879
AspS9I GGNCC 2 cut(s) 46, 827
AsuC2I CCSGG 1 cut(s) 831
AsuHPI GGTGA 3 cut(s) 272, 902, 995
AsuII TTCGAA 2 cut(s) 151, 667
AvaII GGWCC 2 cut(s) 46, 827
BaeGI GKGCMC 1 cut(s) 466
BalI TGGCCA 1 cut(s) 1185
BauI CACGAG 2 cut(s) 465, 714
BbsI GAAGAC 2 cut(s) 552, 1133
BbvI GCAGC 1 cut(s) 1107
BccI CCATC 7 cut(s) 216, 589, 624, 841, 974, 1010, 1157
BclI TGATCA 2 cut(s) 232, 742
BcnI CCSGG 1 cut(s) 831
BcoDI GTCTC 3 cut(s) 407, 729, 1125
BglII AGATCT 1 cut(s) 751
BisI GCNGC 1 cut(s) 1096
BlsI GCNGC 1 cut(s) 1097
Bme1390I CCNGG 1 cut(s) 831
Bme18I GGWCC 2 cut(s) 46, 827
BmgT120I GGNCC 2 cut(s) 46, 827
BmiI GGNNCC 3 cut(s) 416, 828, 945
BmrFI CCNGG 1 cut(s) 831
BmrI ACTGGG 1 cut(s) 651
BmsI GCATC 4 cut(s) 166, 304, 348, 1141
BmuI ACTGGG 1 cut(s) 651
BoxI GACNNNNGTC 1 cut(s) 1137
BpiI GAAGAC 2 cut(s) 552, 1133
Bpu14I TTCGAA 2 cut(s) 151, 667
BpuMI CCSGG 1 cut(s) 831
BsaBI GATNNNNATC 1 cut(s) 1155
BsaI GGTCTC 2 cut(s) 407, 1125
BsaXI ACNNNNNCTCC 2 cut(s) 782, 812
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 459
Bse1I ACTGG 2 cut(s) 657, 1003
Bse3DI GCAATG 2 cut(s) 361, 1027
Bse8I GATNNNNATC 1 cut(s) 1155
BseGI GGATG 3 cut(s) 129, 295, 492
BseJI GATNNNNATC 1 cut(s) 1155
BseLI CCNNNNNNNGG 2 cut(s) 43, 459
BseMI GCAATG 2 cut(s) 361, 1027
BseMII CTCAG 1 cut(s) 1150
BseNI ACTGG 2 cut(s) 657, 1003
BseRI GAGGAG 1 cut(s) 803
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 1 cut(s) 1107
BshFI GGCC 1 cut(s) 1185
BsiSI CCGG 1 cut(s) 830
BslFI GGGAC 1 cut(s) 1084
BslI CCNNNNNNNGG 2 cut(s) 43, 459
BsmAI GTCTC 3 cut(s) 407, 729, 1125
BsmFI GGGAC 1 cut(s) 1084
BsmI GAATGC 1 cut(s) 851
BsnI GGCC 1 cut(s) 1185
Bso31I GGTCTC 2 cut(s) 407, 1125
Bsp119I TTCGAA 2 cut(s) 151, 667
Bsp1286I GDGCHC 1 cut(s) 466
Bsp1407I TGTACA 3 cut(s) 609, 756, 1106
Bsp143I GATC 8 cut(s) 223, 232, 332, 394, 742, 751, 775, 1156
BspACI CCGC 1 cut(s) 41
BspANI GGCC 1 cut(s) 1185
BspCNI CTCAG 1 cut(s) 1149
BspHI TCATGA 1 cut(s) 229
BspLI GGNNCC 3 cut(s) 416, 828, 945
BspPI GGATC 3 cut(s) 218, 340, 1151
BspT104I TTCGAA 2 cut(s) 151, 667
BspTNI GGTCTC 2 cut(s) 407, 1125
BsrDI GCAATG 2 cut(s) 361, 1027
BsrGI TGTACA 3 cut(s) 609, 756, 1106
BsrI ACTGG 2 cut(s) 657, 1003
BssMI GATC 8 cut(s) 223, 232, 332, 394, 742, 751, 775, 1156
BssSI CACGAG 2 cut(s) 465, 714
Bst2BI CACGAG 2 cut(s) 465, 714
Bst4CI ACNGT 2 cut(s) 21, 896
BstAUI TGTACA 3 cut(s) 609, 756, 1106
BstBI TTCGAA 2 cut(s) 151, 667
BstC8I GCNNGC 1 cut(s) 965
BstDEI CTNAG 1 cut(s) 1136
BstENI CCTNNNNNAGG 1 cut(s) 41
BstF5I GGATG 3 cut(s) 129, 295, 492
BstHHI GCGC 1 cut(s) 879
BstKTI GATC 8 cut(s) 226, 235, 335, 397, 745, 754, 778, 1159
BstMAI GTCTC 3 cut(s) 407, 729, 1125
BstMBI GATC 8 cut(s) 223, 232, 332, 394, 742, 751, 775, 1156
BstMWI GCNNNNNNNGC 4 cut(s) 867, 876, 930, 1182
BstPAI GACNNNNGTC 1 cut(s) 1137
BstSCI CCNGG 1 cut(s) 829
BstSLI GKGCMC 1 cut(s) 466
BstV1I GCAGC 1 cut(s) 1107
BstV2I GAAGAC 2 cut(s) 552, 1133
BstX2I RGATCY 2 cut(s) 332, 751
BstYI RGATCY 2 cut(s) 332, 751
BsuRI GGCC 1 cut(s) 1185
BtsCI GGATG 3 cut(s) 129, 295, 492
Cac8I GCNNGC 1 cut(s) 965
CciI TCATGA 1 cut(s) 229
CfoI GCGC 1 cut(s) 879
Cfr13I GGNCC 2 cut(s) 46, 827
Csp6I GTAC 6 cut(s) 34, 490, 610, 757, 1039, 1107
CviAII CATG 9 cut(s) 56, 230, 374, 421, 551, 760, 1013, 1061, 1187
CviQI GTAC 6 cut(s) 34, 490, 610, 757, 1039, 1107
DdeI CTNAG 1 cut(s) 1136
DpnI GATC 8 cut(s) 225, 234, 334, 396, 744, 753, 777, 1158
DpnII GATC 8 cut(s) 223, 232, 332, 394, 742, 751, 775, 1156
DraI TTTAAA 1 cut(s) 603
EaeI YGGCCR 1 cut(s) 1183
Eco31I GGTCTC 2 cut(s) 407, 1125
Eco47I GGWCC 2 cut(s) 46, 827
Eco57I CTGAAG 1 cut(s) 534
EcoNI CCTNNNNNAGG 1 cut(s) 41
EcoO109I RGGNCCY 1 cut(s) 46
EcoRI GAATTC 1 cut(s) 565
FaeI CATG 9 cut(s) 59, 233, 377, 424, 554, 763, 1016, 1064, 1190
FaqI GGGAC 1 cut(s) 1084
FatI CATG 9 cut(s) 55, 229, 373, 420, 550, 759, 1012, 1060, 1186
FauI CCCGC 1 cut(s) 48
FbaI TGATCA 2 cut(s) 232, 742
Fnu4HI GCNGC 1 cut(s) 1096
FokI GGATG 3 cut(s) 116, 282, 499
Fsp4HI GCNGC 1 cut(s) 1096
GlaI GCGC 1 cut(s) 878
GluI GCNGC 1 cut(s) 1096
HaeIII GGCC 1 cut(s) 1185
HapII CCGG 1 cut(s) 830
HhaI GCGC 1 cut(s) 879
Hin1II CATG 9 cut(s) 59, 233, 377, 424, 554, 763, 1016, 1064, 1190
Hin6I GCGC 1 cut(s) 877
HinP1I GCGC 1 cut(s) 877
HincII GTYRAC 1 cut(s) 164
HindII GTYRAC 1 cut(s) 164
HindIII AAGCTT 1 cut(s) 647
HinfI GANTC 6 cut(s) 72, 218, 481, 710, 814, 926
HpaII CCGG 1 cut(s) 830
HphI GGTGA 3 cut(s) 272, 902, 995
Hpy166II GTNNAC 4 cut(s) 164, 610, 912, 1107
Hpy188I TCNGA 6 cut(s) 7, 95, 223, 486, 813, 857
Hpy188III TCNNGA 4 cut(s) 230, 478, 997, 1160
Hpy8I GTNNAC 4 cut(s) 164, 610, 912, 1107
Hpy99I CGWCG 2 cut(s) 1086, 1173
HpyAV CCTTC 4 cut(s) 115, 463, 520, 778
HpyCH4III ACNGT 2 cut(s) 21, 896
HpyCH4IV ACGT 1 cut(s) 272
HpyCH4V TGCA 1 cut(s) 354
HpyF10VI GCNNNNNNNGC 4 cut(s) 867, 876, 930, 1182
HpyF3I CTNAG 1 cut(s) 1136
HpySE526I ACGT 1 cut(s) 272
Hsp92II CATG 9 cut(s) 59, 233, 377, 424, 554, 763, 1016, 1064, 1190
HspAI GCGC 1 cut(s) 877
Ksp22I TGATCA 2 cut(s) 232, 742
Kzo9I GATC 8 cut(s) 223, 232, 332, 394, 742, 751, 775, 1156
LmnI GCTCC 1 cut(s) 943
Lsp1109I GCAGC 1 cut(s) 1107
LweI GCATC 4 cut(s) 166, 304, 348, 1141
MaeII ACGT 1 cut(s) 272
MaeIII GTNAC 3 cut(s) 247, 766, 890
MalI GATC 8 cut(s) 225, 234, 334, 396, 744, 753, 777, 1158
MboI GATC 8 cut(s) 223, 232, 332, 394, 742, 751, 775, 1156
MboII GAAGA 7 cut(s) 290, 293, 326, 527, 557, 656, 1133
MflI RGATCY 2 cut(s) 332, 751
MhlI GDGCHC 1 cut(s) 466
MlsI TGGCCA 1 cut(s) 1185
MluCI AATT 9 cut(s) 60, 438, 473, 565, 586, 669, 746, 958, 1077
MluNI TGGCCA 1 cut(s) 1185
MlyI GAGTC 1 cut(s) 66
MmeI TCCRAC 1 cut(s) 921
MnlI CCTC 9 cut(s) 47, 209, 237, 320, 461, 781, 851, 981, 1145
Mox20I TGGCCA 1 cut(s) 1185
MroXI GAANNNNTTC 1 cut(s) 648
MscI TGGCCA 1 cut(s) 1185
MseI TTAA 6 cut(s) 68, 81, 171, 185, 602, 623
MslI CAYNNNNRTG 3 cut(s) 186, 764, 1065
Msp20I TGGCCA 1 cut(s) 1185
MspI CCGG 1 cut(s) 830
MspR9I CCNGG 1 cut(s) 831
Mva1269I GAATGC 1 cut(s) 851
MwoI GCNNNNNNNGC 4 cut(s) 867, 876, 930, 1182
NciI CCSGG 1 cut(s) 831
NdeII GATC 8 cut(s) 223, 232, 332, 394, 742, 751, 775, 1156
NlaIII CATG 9 cut(s) 59, 233, 377, 424, 554, 763, 1016, 1064, 1190
NlaIV GGNNCC 3 cut(s) 416, 828, 945
NmuCI GTSAC 2 cut(s) 766, 890
NspV TTCGAA 2 cut(s) 151, 667
PagI TCATGA 1 cut(s) 229
PcsI WCGNNNNNNNCGW 1 cut(s) 157
PctI GAATGC 1 cut(s) 851
PdmI GAANNNNTTC 1 cut(s) 648
PfeI GAWTC 5 cut(s) 218, 481, 710, 814, 926
PfoI TCCNGGA 1 cut(s) 829
PkrI GCNGC 1 cut(s) 1097
PleI GAGTC 1 cut(s) 66
PpsI GAGTC 1 cut(s) 66
PpuMI RGGWCCY 1 cut(s) 46
PshAI GACNNNNGTC 1 cut(s) 1137
Psp5II RGGWCCY 1 cut(s) 46
PspN4I GGNNCC 3 cut(s) 416, 828, 945
PspPI GGNCC 2 cut(s) 46, 827
PspPPI RGGWCCY 1 cut(s) 46
PsuI RGATCY 2 cut(s) 332, 751
RsaI GTAC 6 cut(s) 35, 491, 611, 758, 1040, 1108
RsaNI GTAC 6 cut(s) 34, 490, 610, 757, 1039, 1107
RseI CAYNNNNRTG 3 cut(s) 186, 764, 1065
SaqAI TTAA 6 cut(s) 68, 81, 171, 185, 602, 623
SatI GCNGC 1 cut(s) 1096
Sau3AI GATC 8 cut(s) 223, 232, 332, 394, 742, 751, 775, 1156
Sau96I GGNCC 2 cut(s) 46, 827
SchI GAGTC 1 cut(s) 66
ScrFI CCNGG 1 cut(s) 831
SduI GDGCHC 1 cut(s) 466
SfaNI GCATC 4 cut(s) 166, 304, 348, 1141
SfuI TTCGAA 2 cut(s) 151, 667
SinI GGWCC 2 cut(s) 46, 827
SmiMI CAYNNNNRTG 3 cut(s) 186, 764, 1065
Sse9I AATT 9 cut(s) 60, 438, 473, 565, 586, 669, 746, 958, 1077
SsiI CCGC 1 cut(s) 41
StyD4I CCNGG 1 cut(s) 829
TaaI ACNGT 2 cut(s) 21, 896
TaiI ACGT 1 cut(s) 275
TaqI TCGA 6 cut(s) 151, 312, 479, 563, 667, 689
TasI AATT 9 cut(s) 60, 438, 473, 565, 586, 669, 746, 958, 1077
TatI WGTACW 3 cut(s) 609, 756, 1106
TfiI GAWTC 5 cut(s) 218, 481, 710, 814, 926
Tru1I TTAA 6 cut(s) 68, 81, 171, 185, 602, 623
Tru9I TTAA 6 cut(s) 68, 81, 171, 185, 602, 623
TseFI GTSAC 2 cut(s) 766, 890
TseI GCWGC 1 cut(s) 1095
Tsp45I GTSAC 2 cut(s) 766, 890
TspDTI ATGAA 4 cut(s) 72, 136, 362, 719
TspGWI ACGGA 1 cut(s) 507
VpaK11BI GGWCC 2 cut(s) 46, 827
XagI CCTNNNNNAGG 1 cut(s) 41
XapI RAATTY 4 cut(s) 565, 586, 669, 1077
XmnI GAANNNNTTC 1 cut(s) 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.