RLG00000008324

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0012975
Physical Location & Seq
Reverse (-)
28893568 .. 28896771
3204 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008324

Sequence Viewer

Length: 1563 bp
ATGGAAAAGGACAGCAATTCAGCCGCACCCAGTTGGAACCCTTCAAGCTTAATGCAGTCCAATGAGCTCCATTGTGCTCCACAGCAACTTCCCAATTCTTATTTCAGTGCCAATTGGGACAATTCAATGGATCAGAGCGATCCCTTTGAGTCTGCCTTGAGCTCCATGGTGTCCTCACCGGCGGCGTCCAACGCCGTCGGTGCCACCGCCTTTCCCGGAGGAGAAGGCGGTGGAGATATGATAAGAGAAGTAATTGGAAGATTAGGAAGCATTTGCAACTCTGGAGATATATCTTCACTGTCTTACAATAACAGTACTAACAATTCATGCTATAGTACCCCATTGAACTCTTCACCGCCTACTAAGCTCAACTTGTCAATGGTTGATCCACAGATGAGAGGCAGTTTGCCAGTTCCAGCACCCTCATCACACCCAACTTTGGCTCCATTTGCAGCTGACCCTGGTTTTGTAGAGAGGGCTGCAAGGTTTTCCAGCTTTGGAAATTTTGGTGGTGGTCTGAATGGTCAGTTCAATTTGAATGAAGCTGAATTGGCCTGCCGATCAATGCTGAAACTTGATTCAGGCAAGCTTTCCAGAGCTTCCAGCAACCAGTCACTGAGATCTCAAATGGGTGGTGTTCAAGAAAGCATCAAAAGCTCTTCACCCCCAGATGGGAATGCAATTCCTGATAAGAAGTTCAGCAGGTTCTCAAGGTCCTCCACCCCAGAAAATGGTGAATTGGGTGATTCCAGAGAAGGGTCATCAGTTTCTGAGCAGATCCCAGCTGGGGATTTGAGTGCGAAAGCAGAGAATGCTTCCAACTCAAGGAAGAGGAAAGCTGTTCCAAGAGGGAAAGCCAAAGAAACATCTTCATCTCCCTCTGTTAAAAATGGCAAGGCTGTGACTGAAGAGCAACCAAATGCCAAGAGAAGCAAAACTGATGAAGCTTCTGGGAATGAAAAGGAAACTCAAAAGTCAAACAAGGAACCTAATGGAGGTAGCAAAGCTACCAAGGATAGTTCAGAGCCTGTTGAGCCTCCAAAGGACTATATCCATGTCAGAGCCAGAAGGGGTCAGGCTACTGATAGCCATAGTCTGGCAGAAAGAGTTCGAAGAGAGAAAATTAGTGAAAGGATGAAGTTTCTTCAAGATCTTGTTCCTGGTTGCAACAAGGTAACTGGGAAAGCTGTGATGCTAGATGAAATCATAAACTATGTTCAGTCATTGCAGCGCCAAGTCGAGTTTCTTTCAATGAAATTGTCAACTGTGAATCCTAGAATGGATTTGAACATGGAGGCTCTCCTGTCAAAGGAAATCCTTCAATCTCAAGGAGCTTTACAAAATGCTCTTTACCAATTAGATTCCGCAGTCCCCGCCTCATTTCCTTTTGGTTATCAACCCCAGCAACTTCCAGCTTTACATAGCAACGGCATTTCTAATGAGACAGAGACGCAGTTTCCAGTAACTTTAAATGCCTCACTGACTCAAAATTCAACCATGCAATCACCTGCTTTTGATAGATTTGGTGGAGCAAATCTTCAGGTAATTAAAGCAAATATTTGA

Protein Analysis

521

Amino Acids

55.7

Weight (kDa)

6.98

Isoelectric Point (pI)

57.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 363 - 410 2e-06 Helix-loop-helix DNA-binding domain
bHLH_SAC51 PF23173 368 - 413 2.8e-06 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012975)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23690
fragaria_vesca FvH4_4g12760 FvH4_4g12760
malus_domestica MD04G1023500.v1.1
prunus_persica Prupe.1G173300_v2.0.a1 Prupe.1G173300_v2.0.a1
pyrus_communis pycom04g02000
rosa_chinensis RchiOBHm_Chr4g0412071
rosa_laevigata RLG00000008324
rosa_multiflora Rmu_sc0000711.1_g000061 Rmu_sc0000711.1_g000062
rosa_roxburghii Rroxscaffold_5G00356350
rosa_rugosa Rorug04G0106600
rosa_samantha Rh4AG171200 Rh4BG170000 Rh4CG182100 Rh4DG165500
rosa_wichuraiana Rw4G014270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1516
Acc36I ACCTGC 2 cut(s) 693, 1516
AccB1I GGYRCC 1 cut(s) 200
AccB7I CCANNNNNTGG 2 cut(s) 731, 1096
AciI CCGC 7 cut(s) 24, 182, 207, 228, 356, 1365, 1374
AclWI GGATC 4 cut(s) 134, 138, 380, 772
AcsI RAATTY 2 cut(s) 502, 1489
AcuI CTGAAG 2 cut(s) 927, 1523
AcyI GRCGYC 1 cut(s) 185
AfaI GTAC 2 cut(s) 316, 337
AjnI CCWGG 2 cut(s) 460, 1159
AjuI GAANNNNNNNTTGG 4 cut(s) 86, 118, 1227, 1259
Alw21I GWGCWC 3 cut(s) 69, 79, 164
Alw26I GTCTC 2 cut(s) 1436, 1442
AlwI GGATC 4 cut(s) 134, 138, 380, 772
AlwNI CAGNNNCTG 3 cut(s) 616, 770, 1028
AoxI GGCC 1 cut(s) 552
ApeKI GCWGC 3 cut(s) 452, 479, 1228
ApoI RAATTY 2 cut(s) 502, 1489
ArsI GACNNNNNNTTYG 2 cut(s) 449, 481
Asp700I GAANNNNTTC 2 cut(s) 1107, 1317
AspLEI GCGC 1 cut(s) 1233
AspS9I GGNCC 1 cut(s) 714
AsuC2I CCSGG 1 cut(s) 216
AsuHPI GGTGA 6 cut(s) 168, 345, 654, 746, 755, 1497
AsuII TTCGAA 1 cut(s) 1111
AvaII GGWCC 1 cut(s) 714
BanI GGYRCC 1 cut(s) 200
BanII GRGCYC 2 cut(s) 69, 164
Bbv12I GWGCWC 3 cut(s) 69, 79, 164
BbvI GCAGC 3 cut(s) 464, 466, 1240
BccI CCATC 1 cut(s) 665
BceAI ACGGC 2 cut(s) 179, 1444
BciT130I CCWGG 2 cut(s) 462, 1161
BcnI CCSGG 1 cut(s) 216
BcoDI GTCTC 2 cut(s) 1436, 1442
BfaI CTAG 2 cut(s) 1196, 1275
BfmI CTRYAG 1 cut(s) 331
BfoI RGCGCY 1 cut(s) 1234
BfuAI ACCTGC 2 cut(s) 693, 1516
BglII AGATCT 2 cut(s) 620, 1150
BisI GCNGC 5 cut(s) 24, 183, 453, 480, 1229
BlsI GCNGC 5 cut(s) 25, 184, 454, 481, 1230
BmcAI AGTACT 1 cut(s) 316
Bme1390I CCNGG 3 cut(s) 216, 462, 1161
Bme18I GGWCC 1 cut(s) 714
BmgT120I GGNCC 1 cut(s) 714
BmiI GGNNCC 4 cut(s) 38, 202, 444, 987
BmrFI CCNGG 3 cut(s) 216, 462, 1161
BmrI ACTGGG 2 cut(s) 24, 1188
BmsI GCATC 2 cut(s) 657, 1182
BmuI ACTGGG 2 cut(s) 24, 1188
BpmI CTGGAG 1 cut(s) 303
Bpu14I TTCGAA 1 cut(s) 1111
BpuEI CTTGAG 4 cut(s) 178, 694, 808, 1311
BpuMI CCSGG 1 cut(s) 216
BsaHI GRCGYC 1 cut(s) 185
BsaJI CCNNGG 3 cut(s) 165, 460, 1011
BsaXI ACNNNNNCTCC 2 cut(s) 427, 457
Bse118I RCCGGY 1 cut(s) 178
Bse1I ACTGG 5 cut(s) 30, 410, 610, 1183, 1460
Bse3DI GCAATG 1 cut(s) 1223
BseBI CCWGG 2 cut(s) 462, 1161
BseDI CCNNGG 3 cut(s) 165, 460, 1011
BseGI GGATG 1 cut(s) 1140
BseMI GCAATG 1 cut(s) 1223
BseMII CTCAG 2 cut(s) 608, 762
BseNI ACTGG 5 cut(s) 30, 410, 610, 1183, 1460
BseRI GAGGAG 1 cut(s) 234
BseXI GCAGC 3 cut(s) 464, 466, 1240
BseYI CCCAGC 3 cut(s) 781, 785, 1401
BshFI GGCC 1 cut(s) 554
BshNI GGYRCC 1 cut(s) 200
BsiHKAI GWGCWC 3 cut(s) 69, 79, 164
BsiSI CCGG 2 cut(s) 179, 216
BslFI GGGAC 2 cut(s) 131, 1355
BsmAI GTCTC 2 cut(s) 1436, 1442
BsmBI CGTCTC 1 cut(s) 1442
BsmFI GGGAC 2 cut(s) 131, 1355
BsmI GAATGC 2 cut(s) 682, 817
BsnI GGCC 1 cut(s) 554
Bsp119I TTCGAA 1 cut(s) 1111
Bsp1286I GDGCHC 3 cut(s) 69, 79, 164
Bsp143I GATC 7 cut(s) 130, 139, 385, 560, 620, 777, 1150
Bsp19I CCATGG 1 cut(s) 165
BspACI CCGC 7 cut(s) 24, 182, 207, 228, 356, 1365, 1374
BspANI GGCC 1 cut(s) 554
BspCNI CTCAG 2 cut(s) 609, 763
BspLI GGNNCC 4 cut(s) 38, 202, 444, 987
BspMI ACCTGC 2 cut(s) 693, 1516
BspPI GGATC 4 cut(s) 134, 138, 380, 772
BspQI GCTCTTC 2 cut(s) 664, 903
BspT104I TTCGAA 1 cut(s) 1111
BspT107I GGYRCC 1 cut(s) 200
BsrDI GCAATG 1 cut(s) 1223
BsrFI RCCGGY 1 cut(s) 178
BsrI ACTGG 5 cut(s) 30, 410, 610, 1183, 1460
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 3 cut(s) 165, 460, 1011
BssMI GATC 7 cut(s) 130, 139, 385, 560, 620, 777, 1150
BssNI GRCGYC 1 cut(s) 185
BssT1I CCWWGG 2 cut(s) 165, 1011
Bst2UI CCWGG 2 cut(s) 462, 1161
Bst4CI ACNGT 3 cut(s) 300, 314, 1267
Bst6I CTCTTC 5 cut(s) 355, 664, 824, 903, 1108
BstACI GRCGYC 1 cut(s) 185
BstAPI GCANNNNNTGC 1 cut(s) 812
BstBI TTCGAA 1 cut(s) 1111
BstC8I GCNNGC 2 cut(s) 556, 587
BstDEI CTNAG 3 cut(s) 363, 617, 771
BstDSI CCRYGG 1 cut(s) 165
BstENI CCTNNNNNAGG 2 cut(s) 993, 1307
BstF5I GGATG 1 cut(s) 1140
BstH2I RGCGCY 1 cut(s) 1234
BstHHI GCGC 1 cut(s) 1233
BstKTI GATC 7 cut(s) 133, 142, 388, 563, 623, 780, 1153
BstMAI GTCTC 2 cut(s) 1436, 1442
BstMBI GATC 7 cut(s) 130, 139, 385, 560, 620, 777, 1150
BstMWI GCNNNNNNNGC 9 cut(s) 191, 200, 364, 449, 551, 654, 812, 1033, 1373
BstNI CCWGG 2 cut(s) 462, 1161
BstSCI CCNGG 3 cut(s) 214, 460, 1159
BstSFI CTRYAG 1 cut(s) 331
BstV1I GCAGC 3 cut(s) 464, 466, 1240
BstX2I RGATCY 3 cut(s) 620, 777, 1150
BstYI RGATCY 3 cut(s) 620, 777, 1150
BsuRI GGCC 1 cut(s) 554
BtgI CCRYGG 1 cut(s) 165
BtsCI GGATG 1 cut(s) 1140
BtsIMutI CAGTG 4 cut(s) 112, 296, 614, 1478
BveI ACCTGC 2 cut(s) 693, 1516
Cac8I GCNNGC 2 cut(s) 556, 587
CaiI CAGNNNCTG 3 cut(s) 616, 770, 1028
CfoI GCGC 1 cut(s) 1233
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 1 cut(s) 714
CseI GACGC 2 cut(s) 174, 1459
Csp6I GTAC 2 cut(s) 315, 336
CviAII CATG 5 cut(s) 166, 327, 1055, 1291, 1498
CviQI GTAC 2 cut(s) 315, 336
DdeI CTNAG 3 cut(s) 363, 617, 771
DpnI GATC 7 cut(s) 132, 141, 387, 562, 622, 779, 1152
DpnII GATC 7 cut(s) 130, 139, 385, 560, 620, 777, 1150
DraI TTTAAA 1 cut(s) 1470
Eam1104I CTCTTC 5 cut(s) 355, 664, 824, 903, 1108
EarI CTCTTC 5 cut(s) 355, 664, 824, 903, 1108
Ecl136II GAGCTC 2 cut(s) 67, 162
Eco130I CCWWGG 2 cut(s) 165, 1011
Eco24I GRGCYC 2 cut(s) 69, 164
Eco47I GGWCC 1 cut(s) 714
Eco53kI GAGCTC 2 cut(s) 67, 162
Eco57I CTGAAG 2 cut(s) 927, 1523
EcoICRI GAGCTC 2 cut(s) 67, 162
EcoNI CCTNNNNNAGG 2 cut(s) 993, 1307
EcoO109I RGGNCCY 1 cut(s) 714
EcoRII CCWGG 2 cut(s) 460, 1159
EcoT14I CCWWGG 2 cut(s) 165, 1011
EcoT38I GRGCYC 2 cut(s) 69, 164
ErhI CCWWGG 2 cut(s) 165, 1011
Esp3I CGTCTC 1 cut(s) 1442
FaeI CATG 5 cut(s) 169, 330, 1058, 1294, 1501
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FaqI GGGAC 2 cut(s) 131, 1355
FatI CATG 5 cut(s) 165, 326, 1054, 1290, 1497
FauI CCCGC 1 cut(s) 1381
Fnu4HI GCNGC 5 cut(s) 24, 183, 453, 480, 1229
FokI GGATG 1 cut(s) 1147
FriOI GRGCYC 2 cut(s) 69, 164
Fsp4HI GCNGC 5 cut(s) 24, 183, 453, 480, 1229
FspBI CTAG 2 cut(s) 1196, 1275
GlaI GCGC 1 cut(s) 1232
GluI GCNGC 5 cut(s) 24, 183, 453, 480, 1229
GsaI CCCAGC 3 cut(s) 785, 789, 1405
GsuI CTGGAG 1 cut(s) 303
HaeII RGCGCY 1 cut(s) 1234
HaeIII GGCC 1 cut(s) 554
HapII CCGG 2 cut(s) 179, 216
HgaI GACGC 2 cut(s) 174, 1459
HhaI GCGC 1 cut(s) 1233
Hin1I GRCGYC 1 cut(s) 185
Hin1II CATG 5 cut(s) 169, 330, 1058, 1294, 1501
Hin6I GCGC 1 cut(s) 1231
HinP1I GCGC 1 cut(s) 1231
HincII GTYRAC 1 cut(s) 1263
HindII GTYRAC 1 cut(s) 1263
HindIII AAGCTT 3 cut(s) 46, 587, 945
HinfI GANTC 6 cut(s) 149, 578, 746, 1270, 1361, 1483
HpaII CCGG 2 cut(s) 179, 216
HphI GGTGA 6 cut(s) 168, 345, 654, 746, 755, 1497
Hpy166II GTNNAC 1 cut(s) 1263
Hpy188I TCNGA 5 cut(s) 135, 519, 772, 1024, 1061
Hpy188III TCNNGA 6 cut(s) 282, 594, 641, 686, 750, 1148
Hpy8I GTNNAC 1 cut(s) 1263
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 5 cut(s) 51, 218, 749, 1062, 1328
HpyCH4III ACNGT 3 cut(s) 300, 314, 1267
HpyCH4V TGCA 8 cut(s) 55, 276, 452, 482, 680, 1167, 1228, 1501
HpyF10VI GCNNNNNNNGC 9 cut(s) 191, 200, 364, 449, 551, 654, 812, 1033, 1373
HpyF3I CTNAG 3 cut(s) 363, 617, 771
Hsp92I GRCGYC 1 cut(s) 185
Hsp92II CATG 5 cut(s) 169, 330, 1058, 1294, 1501
HspAI GCGC 1 cut(s) 1231
Kzo9I GATC 7 cut(s) 130, 139, 385, 560, 620, 777, 1150
LguI GCTCTTC 2 cut(s) 664, 903
LmnI GCTCC 6 cut(s) 72, 82, 167, 448, 1331, 1529
Lsp1109I GCAGC 3 cut(s) 464, 466, 1240
LweI GCATC 2 cut(s) 657, 1182
MaeI CTAG 2 cut(s) 1196, 1275
MaeIII GTNAC 4 cut(s) 612, 901, 1174, 1462
MalI GATC 7 cut(s) 132, 141, 387, 562, 622, 779, 1152
MboI GATC 7 cut(s) 130, 139, 385, 560, 620, 777, 1150
MfeI CAATTG 1 cut(s) 112
MflI RGATCY 3 cut(s) 620, 777, 1150
MhlI GDGCHC 3 cut(s) 69, 79, 164
MlyI GAGTC 2 cut(s) 158, 1477
MmeI TCCRAC 3 cut(s) 14, 213, 843
MroXI GAANNNNTTC 2 cut(s) 1107, 1317
MseI TTAA 4 cut(s) 50, 885, 1469, 1548
MspA1I CMGCKG 2 cut(s) 455, 785
MspI CCGG 2 cut(s) 179, 216
MspR9I CCNGG 3 cut(s) 216, 462, 1161
MunI CAATTG 1 cut(s) 112
Mva1269I GAATGC 2 cut(s) 682, 817
MvaI CCWGG 2 cut(s) 462, 1161
MwoI GCNNNNNNNGC 9 cut(s) 191, 200, 364, 449, 551, 654, 812, 1033, 1373
NciI CCSGG 1 cut(s) 216
NcoI CCATGG 1 cut(s) 165
NdeII GATC 7 cut(s) 130, 139, 385, 560, 620, 777, 1150
NlaIII CATG 5 cut(s) 169, 330, 1058, 1294, 1501
NlaIV GGNNCC 4 cut(s) 38, 202, 444, 987
NmuCI GTSAC 2 cut(s) 612, 901
NspV TTCGAA 1 cut(s) 1111
PaqCI CACCTGC 1 cut(s) 1516
PciSI GCTCTTC 2 cut(s) 664, 903
PctI GAATGC 2 cut(s) 682, 817
PdmI GAANNNNTTC 2 cut(s) 1107, 1317
PfeI GAWTC 4 cut(s) 578, 746, 1270, 1361
PflMI CCANNNNNTGG 2 cut(s) 731, 1096
PfoI TCCNGGA 1 cut(s) 214
PkrI GCNGC 5 cut(s) 25, 184, 454, 481, 1230
PleI GAGTC 2 cut(s) 157, 1477
PpsI GAGTC 2 cut(s) 157, 1477
PpuMI RGGWCCY 1 cut(s) 714
Psp124BI GAGCTC 2 cut(s) 69, 164
Psp5II RGGWCCY 1 cut(s) 714
Psp6I CCWGG 2 cut(s) 460, 1159
PspFI CCCAGC 3 cut(s) 781, 785, 1401
PspGI CCWGG 2 cut(s) 460, 1159
PspN4I GGNNCC 4 cut(s) 38, 202, 444, 987
PspPI GGNCC 1 cut(s) 714
PspPPI RGGWCCY 1 cut(s) 714
PstNI CAGNNNCTG 3 cut(s) 616, 770, 1028
PsuI RGATCY 3 cut(s) 620, 777, 1150
PvuII CAGCTG 2 cut(s) 455, 785
RsaI GTAC 2 cut(s) 316, 337
RsaNI GTAC 2 cut(s) 315, 336
SacI GAGCTC 2 cut(s) 69, 164
SapI GCTCTTC 2 cut(s) 664, 903
SaqAI TTAA 4 cut(s) 50, 885, 1469, 1548
SatI GCNGC 5 cut(s) 24, 183, 453, 480, 1229
Sau3AI GATC 7 cut(s) 130, 139, 385, 560, 620, 777, 1150
Sau96I GGNCC 1 cut(s) 714
ScaI AGTACT 1 cut(s) 316
SchI GAGTC 2 cut(s) 158, 1477
ScrFI CCNGG 3 cut(s) 216, 462, 1161
SduI GDGCHC 3 cut(s) 69, 79, 164
SfaNI GCATC 2 cut(s) 657, 1182
SfcI CTRYAG 1 cut(s) 331
SfuI TTCGAA 1 cut(s) 1111
SgrAI CRCCGGYG 1 cut(s) 178
SinI GGWCC 1 cut(s) 714
SmlI CTYRAG 4 cut(s) 157, 709, 823, 1326
SmoI CTYRAG 4 cut(s) 157, 709, 823, 1326
SsiI CCGC 7 cut(s) 24, 182, 207, 228, 356, 1365, 1374
SspI AATATT 1 cut(s) 1558
SspMI CTAG 2 cut(s) 1196, 1275
SstI GAGCTC 2 cut(s) 69, 164
StyD4I CCNGG 3 cut(s) 214, 460, 1159
StyI CCWWGG 2 cut(s) 165, 1011
TaaI ACNGT 3 cut(s) 300, 314, 1267
TaqI TCGA 2 cut(s) 1111, 1239
TatI WGTACW 1 cut(s) 314
TauI GCSGC 2 cut(s) 26, 185
TfiI GAWTC 4 cut(s) 578, 746, 1270, 1361
Tru1I TTAA 4 cut(s) 50, 885, 1469, 1548
Tru9I TTAA 4 cut(s) 50, 885, 1469, 1548
TscAI CASTG 4 cut(s) 112, 303, 621, 1485
TseFI GTSAC 2 cut(s) 612, 901
TseI GCWGC 3 cut(s) 452, 479, 1228
Tsp45I GTSAC 2 cut(s) 612, 901
TspDTI ATGAA 8 cut(s) 315, 555, 861, 957, 972, 1151, 1215, 1268
TspRI CASTG 4 cut(s) 112, 303, 621, 1485
Van91I CCANNNNNTGG 2 cut(s) 731, 1096
VpaK11BI GGWCC 1 cut(s) 714
XagI CCTNNNNNAGG 2 cut(s) 993, 1307
XapI RAATTY 2 cut(s) 502, 1489
XmnI GAANNNNTTC 2 cut(s) 1107, 1317
XspI CTAG 2 cut(s) 1196, 1275
ZrmI AGTACT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.