Rh4DG165500

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0012975
Physical Location & Seq
Forward (+)
32979381 .. 32982883
3503 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG165500.1

Sequence Viewer

Length: 1566 bp
ATGGAAAAGGACAGCAACTCAGCCGCACCCAGTTGGAACCCTTCAAGCTTAATGCAGTCCAATGAGCTCCATTGTGCTCCACAGCAACTTCCCAATTCTTATTTCAATGCCAATTGGGACAATTCAATGGATCAGAGCGATCCCTTTGAGTCTGCCTTGAGCTCCATGGTGTCCTCACCGGCGGCGTCCAACGCCGTCGGTGCCACCGCCTTTCCCGGAGGAGAAGGCGGTGGAGATATGATAAGAGAAGTAATTGGAAGATTAGGCAGCATTTGCAACTCTGGAGATATATCTTCACTGTCTTACAATAACAGTACTAACAATTCATGCTATAGTACCCCATTAAACTCTTCACCGCCTACTAAGCTCAACTTGTCAATGGCTGATCCACAGATGAGAGGCAATTTTCCAGTTCCAGCACCCTCATCACACCCAACTTTGGCTCCATTTGCAGCTGACCCTGGTTTTGTAGAGAGGGCAGCAAGGTTTTCCAGCTTTGGAAATTTTGGTGGTGGTCTGAATGGTCAGTTCAATTTGAATGAAGCTGAATTGGCCTACAGATCAATGCTGAAACTTGATTCAGGCAAGCTTTCCAGAGCTTCCAGCAACCAGTCACTGAGATCTCAAATGGGTGGTGTTCAAGAAAGCAGCATCAAAAGCTCTTCACCCCAAGATGGGAATGCAATTCCTGATAAGAAGTTCAGCAGGTTCTCAAGGTCCTCCACCCCAGAAAATGGTGAATTGGGTGATTCCAGAGAAGGGTCATCAGTTTCTGAGCAGATCCCAGCTGGGGATTTGAGTGTGAAAGCAGAGAATGCTTCCAACTCAAGGAAGAGGAAAGCTGTTCCAAGAGGGAAAGCCAAAGAAACCTCTTCATCTCCCTCTGTTAAAAATGGCAAGGCTGTGACTGAAGAGCAACCAAATGCCAAGAGAAGCAAAACTGATGAAGCTTCTGGGAATGAAAAGGAAACTCAAAAGTCAAACAAGGAACCTAATGGAGGTAGCAAAACTACCAAGGATAGTTCAGAGCCTGTTGAGCCTCCAAAGGACTATATCCATGTCAGAGCCAGAAGGGGTCAGGCTACTGATAGCCATAGTCTGGCAGAAAGAGTTCGAAGAGAGAAAATTAGTGAAAGGATGAAGTTTCTTCAAGATCTTGTTCCTGGTTGCAACAAGGTAACTGGGAAAGCTGTGATGCTAGATGAAATCATTAACTATGTTCAGTCGTTGCAGCGCCAAGTCGAGTTTCTTTCAATGAAATTGTCAACTGTGAATCCTAGAATGGATTTGAACATGGAGGCTCTCCTGTCAAAGGAAATCCTTCAATCTCAAGGAGCTTTACAAAATGCTCTTTACCAATTAGATTCCGCAGTCCCCGCATCATTTCCTTTTGGGTATCAACCCCAGCAACTTCCAGCTTTACATAGCAACGGCATCTCTAATGAGACAGAGACGCAGTTTCCAGTAACTTTGAATGCCTCACTGACTCAAAATTCAACCATGCAATCACCTGCTTTTGATAGATTTGGTGGAGCAAATCTTCAGGTAATTAAAGCAAATATTTGA

Protein Analysis

521

Amino Acids

55.99

Weight (kDa)

6.99

Isoelectric Point (pI)

57.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 364 - 411 2e-06 Helix-loop-helix DNA-binding domain
bHLH_SAC51 PF23173 369 - 414 2.8e-06 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012975)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23690
fragaria_vesca FvH4_4g12760 FvH4_4g12760
malus_domestica MD04G1023500.v1.1
prunus_persica Prupe.1G173300_v2.0.a1 Prupe.1G173300_v2.0.a1
pyrus_communis pycom04g02000
rosa_chinensis RchiOBHm_Chr4g0412071
rosa_laevigata RLG00000008324
rosa_multiflora Rmu_sc0000711.1_g000061 Rmu_sc0000711.1_g000062
rosa_roxburghii Rroxscaffold_5G00356350
rosa_rugosa Rorug04G0106600
rosa_samantha Rh4AG171200 Rh4BG170000 Rh4CG182100 Rh4DG165500
rosa_wichuraiana Rw4G014270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1519
Acc36I ACCTGC 2 cut(s) 696, 1519
AccB1I GGYRCC 1 cut(s) 200
AccB7I CCANNNNNTGG 2 cut(s) 734, 1099
AciI CCGC 7 cut(s) 24, 182, 207, 228, 356, 1368, 1377
AclWI GGATC 4 cut(s) 134, 138, 380, 775
AcsI RAATTY 2 cut(s) 502, 1492
AcuI CTGAAG 2 cut(s) 930, 1526
AcyI GRCGYC 1 cut(s) 185
AfaI GTAC 2 cut(s) 316, 337
AjnI CCWGG 2 cut(s) 460, 1162
AjuI GAANNNNNNNTTGG 4 cut(s) 86, 118, 1230, 1262
Alw21I GWGCWC 3 cut(s) 69, 79, 164
Alw26I GTCTC 2 cut(s) 1439, 1445
AlwI GGATC 4 cut(s) 134, 138, 380, 775
AlwNI CAGNNNCTG 3 cut(s) 616, 773, 1031
AoxI GGCC 1 cut(s) 552
ApeKI GCWGC 5 cut(s) 267, 452, 479, 648, 1231
ApoI RAATTY 2 cut(s) 502, 1492
ArsI GACNNNNNNTTYG 2 cut(s) 449, 481
Asp700I GAANNNNTTC 2 cut(s) 1110, 1320
AspLEI GCGC 1 cut(s) 1236
AspS9I GGNCC 1 cut(s) 717
AsuC2I CCSGG 1 cut(s) 216
AsuHPI GGTGA 6 cut(s) 168, 345, 657, 749, 758, 1500
AsuII TTCGAA 1 cut(s) 1114
AvaII GGWCC 1 cut(s) 717
BanI GGYRCC 1 cut(s) 200
BanII GRGCYC 2 cut(s) 69, 164
Bbv12I GWGCWC 3 cut(s) 69, 79, 164
BbvI GCAGC 5 cut(s) 279, 464, 491, 660, 1243
BccI CCATC 1 cut(s) 668
BceAI ACGGC 2 cut(s) 179, 1447
BciT130I CCWGG 2 cut(s) 462, 1164
BcnI CCSGG 1 cut(s) 216
BcoDI GTCTC 2 cut(s) 1439, 1445
BfaI CTAG 2 cut(s) 1199, 1278
BfmI CTRYAG 2 cut(s) 331, 556
BfoI RGCGCY 1 cut(s) 1237
BfuAI ACCTGC 2 cut(s) 696, 1519
BglII AGATCT 2 cut(s) 620, 1153
BisI GCNGC 7 cut(s) 24, 183, 268, 453, 480, 649, 1232
BlsI GCNGC 7 cut(s) 25, 184, 269, 454, 481, 650, 1233
BmcAI AGTACT 1 cut(s) 316
Bme1390I CCNGG 3 cut(s) 216, 462, 1164
Bme18I GGWCC 1 cut(s) 717
BmgT120I GGNCC 1 cut(s) 717
BmiI GGNNCC 4 cut(s) 38, 202, 444, 990
BmrFI CCNGG 3 cut(s) 216, 462, 1164
BmrI ACTGGG 2 cut(s) 24, 1191
BmsI GCATC 4 cut(s) 660, 1185, 1388, 1443
BmuI ACTGGG 2 cut(s) 24, 1191
BpmI CTGGAG 1 cut(s) 303
Bpu14I TTCGAA 1 cut(s) 1114
BpuEI CTTGAG 4 cut(s) 178, 697, 811, 1314
BpuMI CCSGG 1 cut(s) 216
BsaHI GRCGYC 1 cut(s) 185
BsaJI CCNNGG 3 cut(s) 165, 460, 1014
BsaXI ACNNNNNCTCC 2 cut(s) 427, 457
Bse118I RCCGGY 1 cut(s) 178
Bse1I ACTGG 5 cut(s) 30, 410, 610, 1186, 1463
BseBI CCWGG 2 cut(s) 462, 1164
BseDI CCNNGG 3 cut(s) 165, 460, 1014
BseGI GGATG 1 cut(s) 1143
BseMII CTCAG 3 cut(s) 33, 608, 765
BseNI ACTGG 5 cut(s) 30, 410, 610, 1186, 1463
BseRI GAGGAG 1 cut(s) 234
BseXI GCAGC 5 cut(s) 279, 464, 491, 660, 1243
BseYI CCCAGC 3 cut(s) 784, 788, 1404
BshFI GGCC 1 cut(s) 554
BshNI GGYRCC 1 cut(s) 200
BsiHKAI GWGCWC 3 cut(s) 69, 79, 164
BsiSI CCGG 2 cut(s) 179, 216
BslFI GGGAC 2 cut(s) 131, 1358
BsmAI GTCTC 2 cut(s) 1439, 1445
BsmBI CGTCTC 1 cut(s) 1445
BsmFI GGGAC 2 cut(s) 131, 1358
BsmI GAATGC 3 cut(s) 685, 820, 1480
BsnI GGCC 1 cut(s) 554
Bsp119I TTCGAA 1 cut(s) 1114
Bsp1286I GDGCHC 3 cut(s) 69, 79, 164
Bsp143I GATC 7 cut(s) 130, 139, 385, 560, 620, 780, 1153
Bsp19I CCATGG 1 cut(s) 165
BspACI CCGC 7 cut(s) 24, 182, 207, 228, 356, 1368, 1377
BspANI GGCC 1 cut(s) 554
BspCNI CTCAG 3 cut(s) 32, 609, 766
BspLI GGNNCC 4 cut(s) 38, 202, 444, 990
BspMI ACCTGC 2 cut(s) 696, 1519
BspPI GGATC 4 cut(s) 134, 138, 380, 775
BspQI GCTCTTC 2 cut(s) 667, 906
BspT104I TTCGAA 1 cut(s) 1114
BspT107I GGYRCC 1 cut(s) 200
BsrFI RCCGGY 1 cut(s) 178
BsrI ACTGG 5 cut(s) 30, 410, 610, 1186, 1463
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 3 cut(s) 165, 460, 1014
BssMI GATC 7 cut(s) 130, 139, 385, 560, 620, 780, 1153
BssNI GRCGYC 1 cut(s) 185
BssT1I CCWWGG 2 cut(s) 165, 1014
Bst2UI CCWGG 2 cut(s) 462, 1164
Bst4CI ACNGT 3 cut(s) 300, 314, 1270
Bst6I CTCTTC 6 cut(s) 355, 667, 827, 877, 906, 1111
BstACI GRCGYC 1 cut(s) 185
BstAPI GCANNNNNTGC 2 cut(s) 273, 815
BstBI TTCGAA 1 cut(s) 1114
BstC8I GCNNGC 1 cut(s) 587
BstDEI CTNAG 4 cut(s) 19, 363, 617, 774
BstDSI CCRYGG 1 cut(s) 165
BstENI CCTNNNNNAGG 2 cut(s) 996, 1310
BstF5I GGATG 1 cut(s) 1143
BstH2I RGCGCY 1 cut(s) 1237
BstHHI GCGC 1 cut(s) 1236
BstKTI GATC 7 cut(s) 133, 142, 388, 563, 623, 783, 1156
BstMAI GTCTC 2 cut(s) 1439, 1445
BstMBI GATC 7 cut(s) 130, 139, 385, 560, 620, 780, 1153
BstNI CCWGG 2 cut(s) 462, 1164
BstSCI CCNGG 3 cut(s) 214, 460, 1162
BstSFI CTRYAG 2 cut(s) 331, 556
BstV1I GCAGC 5 cut(s) 279, 464, 491, 660, 1243
BstX2I RGATCY 3 cut(s) 620, 780, 1153
BstYI RGATCY 3 cut(s) 620, 780, 1153
BsuRI GGCC 1 cut(s) 554
BtgI CCRYGG 1 cut(s) 165
BtsCI GGATG 1 cut(s) 1143
BtsIMutI CAGTG 3 cut(s) 296, 614, 1481
BveI ACCTGC 2 cut(s) 696, 1519
Cac8I GCNNGC 1 cut(s) 587
CaiI CAGNNNCTG 3 cut(s) 616, 773, 1031
CfoI GCGC 1 cut(s) 1236
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 1 cut(s) 717
CseI GACGC 2 cut(s) 174, 1462
Csp6I GTAC 2 cut(s) 315, 336
CviAII CATG 5 cut(s) 166, 327, 1058, 1294, 1501
CviQI GTAC 2 cut(s) 315, 336
DdeI CTNAG 4 cut(s) 19, 363, 617, 774
DpnI GATC 7 cut(s) 132, 141, 387, 562, 622, 782, 1155
DpnII GATC 7 cut(s) 130, 139, 385, 560, 620, 780, 1153
Eam1104I CTCTTC 6 cut(s) 355, 667, 827, 877, 906, 1111
EarI CTCTTC 6 cut(s) 355, 667, 827, 877, 906, 1111
Ecl136II GAGCTC 2 cut(s) 67, 162
Eco130I CCWWGG 2 cut(s) 165, 1014
Eco24I GRGCYC 2 cut(s) 69, 164
Eco47I GGWCC 1 cut(s) 717
Eco53kI GAGCTC 2 cut(s) 67, 162
Eco57I CTGAAG 2 cut(s) 930, 1526
EcoICRI GAGCTC 2 cut(s) 67, 162
EcoNI CCTNNNNNAGG 2 cut(s) 996, 1310
EcoO109I RGGNCCY 1 cut(s) 717
EcoRII CCWGG 2 cut(s) 460, 1162
EcoT14I CCWWGG 2 cut(s) 165, 1014
EcoT38I GRGCYC 2 cut(s) 69, 164
ErhI CCWWGG 2 cut(s) 165, 1014
Esp3I CGTCTC 1 cut(s) 1445
FaeI CATG 5 cut(s) 169, 330, 1061, 1297, 1504
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FaqI GGGAC 2 cut(s) 131, 1358
FatI CATG 5 cut(s) 165, 326, 1057, 1293, 1500
FauI CCCGC 1 cut(s) 1384
Fnu4HI GCNGC 7 cut(s) 24, 183, 268, 453, 480, 649, 1232
FokI GGATG 1 cut(s) 1150
FriOI GRGCYC 2 cut(s) 69, 164
Fsp4HI GCNGC 7 cut(s) 24, 183, 268, 453, 480, 649, 1232
FspBI CTAG 2 cut(s) 1199, 1278
GlaI GCGC 1 cut(s) 1235
GluI GCNGC 7 cut(s) 24, 183, 268, 453, 480, 649, 1232
GsaI CCCAGC 3 cut(s) 788, 792, 1408
GsuI CTGGAG 1 cut(s) 303
HaeII RGCGCY 1 cut(s) 1237
HaeIII GGCC 1 cut(s) 554
HapII CCGG 2 cut(s) 179, 216
HgaI GACGC 2 cut(s) 174, 1462
HhaI GCGC 1 cut(s) 1236
Hin1I GRCGYC 1 cut(s) 185
Hin1II CATG 5 cut(s) 169, 330, 1061, 1297, 1504
Hin6I GCGC 1 cut(s) 1234
HinP1I GCGC 1 cut(s) 1234
HincII GTYRAC 1 cut(s) 1266
HindII GTYRAC 1 cut(s) 1266
HindIII AAGCTT 3 cut(s) 46, 587, 948
HinfI GANTC 6 cut(s) 149, 578, 749, 1273, 1364, 1486
HpaII CCGG 2 cut(s) 179, 216
HphI GGTGA 6 cut(s) 168, 345, 657, 749, 758, 1500
Hpy166II GTNNAC 1 cut(s) 1266
Hpy188I TCNGA 5 cut(s) 135, 519, 775, 1027, 1064
Hpy188III TCNNGA 6 cut(s) 282, 594, 641, 689, 753, 1151
Hpy8I GTNNAC 1 cut(s) 1266
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 5 cut(s) 51, 218, 752, 1065, 1331
HpyCH4III ACNGT 3 cut(s) 300, 314, 1270
HpyCH4V TGCA 7 cut(s) 55, 276, 452, 683, 1170, 1231, 1504
HpyF3I CTNAG 4 cut(s) 19, 363, 617, 774
Hsp92I GRCGYC 1 cut(s) 185
Hsp92II CATG 5 cut(s) 169, 330, 1061, 1297, 1504
HspAI GCGC 1 cut(s) 1234
Kzo9I GATC 7 cut(s) 130, 139, 385, 560, 620, 780, 1153
LguI GCTCTTC 2 cut(s) 667, 906
LmnI GCTCC 6 cut(s) 72, 82, 167, 448, 1334, 1532
Lsp1109I GCAGC 5 cut(s) 279, 464, 491, 660, 1243
LweI GCATC 4 cut(s) 660, 1185, 1388, 1443
MaeI CTAG 2 cut(s) 1199, 1278
MaeIII GTNAC 4 cut(s) 612, 904, 1177, 1465
MalI GATC 7 cut(s) 132, 141, 387, 562, 622, 782, 1155
MboI GATC 7 cut(s) 130, 139, 385, 560, 620, 780, 1153
MfeI CAATTG 1 cut(s) 112
MflI RGATCY 3 cut(s) 620, 780, 1153
MhlI GDGCHC 3 cut(s) 69, 79, 164
MlyI GAGTC 2 cut(s) 158, 1480
MmeI TCCRAC 3 cut(s) 14, 213, 846
MroXI GAANNNNTTC 2 cut(s) 1110, 1320
MseI TTAA 5 cut(s) 50, 344, 888, 1212, 1551
MspA1I CMGCKG 2 cut(s) 455, 788
MspI CCGG 2 cut(s) 179, 216
MspR9I CCNGG 3 cut(s) 216, 462, 1164
MunI CAATTG 1 cut(s) 112
Mva1269I GAATGC 3 cut(s) 685, 820, 1480
MvaI CCWGG 2 cut(s) 462, 1164
NciI CCSGG 1 cut(s) 216
NcoI CCATGG 1 cut(s) 165
NdeII GATC 7 cut(s) 130, 139, 385, 560, 620, 780, 1153
NlaIII CATG 5 cut(s) 169, 330, 1061, 1297, 1504
NlaIV GGNNCC 4 cut(s) 38, 202, 444, 990
NmuCI GTSAC 2 cut(s) 612, 904
NspV TTCGAA 1 cut(s) 1114
PaqCI CACCTGC 1 cut(s) 1519
PciSI GCTCTTC 2 cut(s) 667, 906
PctI GAATGC 3 cut(s) 685, 820, 1480
PdmI GAANNNNTTC 2 cut(s) 1110, 1320
PfeI GAWTC 4 cut(s) 578, 749, 1273, 1364
PflMI CCANNNNNTGG 2 cut(s) 734, 1099
PfoI TCCNGGA 1 cut(s) 214
PkrI GCNGC 7 cut(s) 25, 184, 269, 454, 481, 650, 1233
PleI GAGTC 2 cut(s) 157, 1480
PpsI GAGTC 2 cut(s) 157, 1480
PpuMI RGGWCCY 1 cut(s) 717
Psp124BI GAGCTC 2 cut(s) 69, 164
Psp5II RGGWCCY 1 cut(s) 717
Psp6I CCWGG 2 cut(s) 460, 1162
PspFI CCCAGC 3 cut(s) 784, 788, 1404
PspGI CCWGG 2 cut(s) 460, 1162
PspN4I GGNNCC 4 cut(s) 38, 202, 444, 990
PspPI GGNCC 1 cut(s) 717
PspPPI RGGWCCY 1 cut(s) 717
PstNI CAGNNNCTG 3 cut(s) 616, 773, 1031
PsuI RGATCY 3 cut(s) 620, 780, 1153
PvuII CAGCTG 2 cut(s) 455, 788
RsaI GTAC 2 cut(s) 316, 337
RsaNI GTAC 2 cut(s) 315, 336
SacI GAGCTC 2 cut(s) 69, 164
SapI GCTCTTC 2 cut(s) 667, 906
SaqAI TTAA 5 cut(s) 50, 344, 888, 1212, 1551
SatI GCNGC 7 cut(s) 24, 183, 268, 453, 480, 649, 1232
Sau3AI GATC 7 cut(s) 130, 139, 385, 560, 620, 780, 1153
Sau96I GGNCC 1 cut(s) 717
ScaI AGTACT 1 cut(s) 316
SchI GAGTC 2 cut(s) 158, 1480
ScrFI CCNGG 3 cut(s) 216, 462, 1164
SduI GDGCHC 3 cut(s) 69, 79, 164
SfaNI GCATC 4 cut(s) 660, 1185, 1388, 1443
SfcI CTRYAG 2 cut(s) 331, 556
SfuI TTCGAA 1 cut(s) 1114
SgrAI CRCCGGYG 1 cut(s) 178
SinI GGWCC 1 cut(s) 717
SmlI CTYRAG 4 cut(s) 157, 712, 826, 1329
SmoI CTYRAG 4 cut(s) 157, 712, 826, 1329
SsiI CCGC 7 cut(s) 24, 182, 207, 228, 356, 1368, 1377
SspI AATATT 1 cut(s) 1561
SspMI CTAG 2 cut(s) 1199, 1278
SstI GAGCTC 2 cut(s) 69, 164
StyD4I CCNGG 3 cut(s) 214, 460, 1162
StyI CCWWGG 2 cut(s) 165, 1014
TaaI ACNGT 3 cut(s) 300, 314, 1270
TaqI TCGA 2 cut(s) 1114, 1242
TatI WGTACW 1 cut(s) 314
TauI GCSGC 2 cut(s) 26, 185
TfiI GAWTC 4 cut(s) 578, 749, 1273, 1364
Tru1I TTAA 5 cut(s) 50, 344, 888, 1212, 1551
Tru9I TTAA 5 cut(s) 50, 344, 888, 1212, 1551
TscAI CASTG 3 cut(s) 303, 621, 1488
TseFI GTSAC 2 cut(s) 612, 904
TseI GCWGC 5 cut(s) 267, 452, 479, 648, 1231
Tsp45I GTSAC 2 cut(s) 612, 904
TspDTI ATGAA 8 cut(s) 315, 555, 864, 960, 975, 1154, 1218, 1271
TspRI CASTG 3 cut(s) 303, 621, 1488
Van91I CCANNNNNTGG 2 cut(s) 734, 1099
VpaK11BI GGWCC 1 cut(s) 717
XagI CCTNNNNNAGG 2 cut(s) 996, 1310
XapI RAATTY 2 cut(s) 502, 1492
XmnI GAANNNNTTC 2 cut(s) 1110, 1320
XspI CTAG 2 cut(s) 1199, 1278
ZrmI AGTACT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.