RLG00000009352

Ripening-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0018475
Physical Location & Seq
Reverse (-)
51416453 .. 51417013
561 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009352

Sequence Viewer

Length: 561 bp
ATGAAGAGCTTCCTTTCAAGAGAGTCTATTCTTGTCATTTTGCTTTTAGCGATTTGCTTTGTTAGTGAAGCTCAGAAATGTTGTCCAAGTGGAATGATAAGAGGAAAGAAGTCCCCTTCTGGACAATGTAACCAGGAGCAAGACCCTGACTGTTGTAGACCGGGAAAAATGTACCCAACCTACTCATGCTCACCCCCAGTGTCCAAGAACACCCACGCCTACCTCACTCTCAACAGTTTCGAGGCAGGTGGTGATGGCAGTCTTCCATCAAAATGCGATAACAAATACCACAATGACAGCACTCCAATCGTAGCACTCTCCACCGGATGGTACAAGAATGGAGGAAGGTGCCATAACAACATTAAAATTACTACTAATGGTCGCAGTGTGGTGGCCATGGTGGTGGATGAATGTGACTCTACTAGGGGATGTGATGCAAATCATGACTACCTACCTCCTTGTGCTAACGACATTATTGTTGCCTCCAAGGCTGTTTGGGAAGGCTTGCATGTGTCTAAAGAAAATTGGGGCAGCTTAGATATCACATGGAATGACGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

187

Amino Acids

20.27

Weight (kDa)

6.49

Isoelectric Point (pI)

48.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KWL1 PF24300 14 - 186 4e-55 Kiwellin-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018475)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g13961
rosa_chinensis RchiOBHm_Chr1g0354461
rosa_laevigata RLG00000009352 RLG00000028215
rosa_multiflora Rmu_sc0002737.1_g000007
rosa_roxburghii Rroxscaffold_4G00301020
rosa_rugosa Rorug01G0243100
rosa_samantha Rh1AG253700 Rh1DG252600
rosa_wichuraiana Rw1G022210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 236
Acc36I ACCTGC 1 cut(s) 236
AccB1I GGYRCC 1 cut(s) 348
AccB7I CCANNNNNTGG 1 cut(s) 327
AccI GTMKAC 1 cut(s) 157
AcoI YGGCCR 1 cut(s) 393
AcyI GRCGYC 1 cut(s) 555
AfaI GTAC 2 cut(s) 173, 332
AfiI CCNNNNNNNGG 1 cut(s) 327
AgsI TTSAA 1 cut(s) 18
AjnI CCWGG 1 cut(s) 132
AluBI AGCT 3 cut(s) 9, 71, 534
AluI AGCT 3 cut(s) 9, 71, 534
AoxI GGCC 1 cut(s) 393
ApeKI GCWGC 1 cut(s) 531
Asp700I GAANNNNTTC 1 cut(s) 8
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 2 cut(s) 183, 263
BalI TGGCCA 1 cut(s) 395
BanI GGYRCC 1 cut(s) 348
BbsI GAAGAC 1 cut(s) 254
BbvI GCAGC 1 cut(s) 543
BccI CCATC 3 cut(s) 248, 274, 321
BcgI CGANNNNNNTGC 2 cut(s) 289, 323
BciT130I CCWGG 1 cut(s) 134
BcnI CCSGG 1 cut(s) 162
BfaI CTAG 1 cut(s) 423
BfuAI ACCTGC 1 cut(s) 236
BglI GCCNNNNNGGC 1 cut(s) 488
BisI GCNGC 1 cut(s) 532
BlsI GCNGC 1 cut(s) 533
Bme1390I CCNGG 2 cut(s) 134, 162
BmiI GGNNCC 1 cut(s) 350
BmrFI CCNGG 2 cut(s) 134, 162
BmrI ACTGGG 1 cut(s) 191
BmsI GCATC 1 cut(s) 424
BmuI ACTGGG 1 cut(s) 191
BpiI GAAGAC 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 162
BsaBI GATNNNNATC 1 cut(s) 438
BsaHI GRCGYC 1 cut(s) 555
BsaJI CCNNGG 2 cut(s) 396, 486
BsaWI WCCGGW 1 cut(s) 323
Bsc4I CCNNNNNNNGG 1 cut(s) 327
Bse1I ACTGG 1 cut(s) 197
Bse8I GATNNNNATC 1 cut(s) 438
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 2 cut(s) 396, 486
BseGI GGATG 3 cut(s) 332, 412, 434
BseJI GATNNNNATC 1 cut(s) 438
BseLI CCNNNNNNNGG 1 cut(s) 327
BseMII CTCAG 1 cut(s) 86
BseNI ACTGG 1 cut(s) 197
BseXI GCAGC 1 cut(s) 543
BshFI GGCC 1 cut(s) 395
BshNI GGYRCC 1 cut(s) 348
BsiSI CCGG 2 cut(s) 161, 324
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 1 cut(s) 327
BsmFI GGGAC 1 cut(s) 97
BsnI GGCC 1 cut(s) 395
Bsp19I CCATGG 1 cut(s) 396
BspANI GGCC 1 cut(s) 395
BspCNI CTCAG 1 cut(s) 85
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 1 cut(s) 350
BspMI ACCTGC 1 cut(s) 236
BspT107I GGYRCC 1 cut(s) 348
BsrI ACTGG 1 cut(s) 197
BssECI CCNNGG 2 cut(s) 396, 486
BssNI GRCGYC 1 cut(s) 555
BssT1I CCWWGG 2 cut(s) 396, 486
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 2 cut(s) 152, 236
BstACI GRCGYC 1 cut(s) 555
BstC8I GCNNGC 1 cut(s) 506
BstDEI CTNAG 2 cut(s) 72, 535
BstDSI CCRYGG 1 cut(s) 396
BstF5I GGATG 3 cut(s) 332, 412, 434
BstMWI GCNNNNNNNGC 1 cut(s) 488
BstNI CCWGG 1 cut(s) 134
BstNSI RCATGY 1 cut(s) 512
BstSCI CCNGG 2 cut(s) 132, 160
BstV1I GCAGC 1 cut(s) 543
BstV2I GAAGAC 1 cut(s) 254
BstXI CCANNNNNNTGG 1 cut(s) 403
BsuRI GGCC 1 cut(s) 395
BtgI CCRYGG 1 cut(s) 396
BtsCI GGATG 3 cut(s) 332, 412, 434
BtsI GCAGTG 1 cut(s) 391
BtsIMutI CAGTG 2 cut(s) 204, 391
BveI ACCTGC 1 cut(s) 236
Cac8I GCNNGC 1 cut(s) 506
CciI TCATGA 1 cut(s) 442
Csp6I GTAC 2 cut(s) 172, 331
CviAII CATG 5 cut(s) 186, 397, 443, 509, 546
CviJI RGCY 6 cut(s) 9, 71, 395, 491, 504, 534
CviKI_1 RGCY 6 cut(s) 9, 71, 395, 491, 504, 534
CviQI GTAC 2 cut(s) 172, 331
DdeI CTNAG 2 cut(s) 72, 535
EaeI YGGCCR 1 cut(s) 393
Eco130I CCWWGG 2 cut(s) 396, 486
Eco32I GATATC 1 cut(s) 541
EcoRII CCWGG 1 cut(s) 132
EcoRV GATATC 1 cut(s) 541
EcoT14I CCWWGG 2 cut(s) 396, 486
ErhI CCWWGG 2 cut(s) 396, 486
FaeI CATG 5 cut(s) 189, 400, 446, 512, 549
FaiI YATR 6 cut(s) 187, 354, 398, 444, 510, 547
FaqI GGGAC 1 cut(s) 97
FatI CATG 5 cut(s) 185, 396, 442, 508, 545
FblI GTMKAC 1 cut(s) 157
Fnu4HI GCNGC 1 cut(s) 532
FokI GGATG 3 cut(s) 339, 419, 441
Fsp4HI GCNGC 1 cut(s) 532
FspBI CTAG 1 cut(s) 423
GluI GCNGC 1 cut(s) 532
HaeIII GGCC 1 cut(s) 395
HapII CCGG 2 cut(s) 161, 324
Hin1I GRCGYC 1 cut(s) 555
Hin1II CATG 5 cut(s) 189, 400, 446, 512, 549
HinfI GANTC 2 cut(s) 23, 416
HpaII CCGG 2 cut(s) 161, 324
HphI GGTGA 2 cut(s) 183, 263
Hpy166II GTNNAC 1 cut(s) 158
Hpy188I TCNGA 1 cut(s) 75
Hpy188III TCNNGA 3 cut(s) 18, 120, 443
Hpy8I GTNNAC 1 cut(s) 158
HpyAV CCTTC 3 cut(s) 126, 339, 494
HpyCH4III ACNGT 2 cut(s) 152, 236
HpyCH4V TGCA 2 cut(s) 437, 508
HpyF10VI GCNNNNNNNGC 1 cut(s) 488
HpyF3I CTNAG 2 cut(s) 72, 535
Hsp92I GRCGYC 1 cut(s) 555
Hsp92II CATG 5 cut(s) 189, 400, 446, 512, 549
LmnI GCTCC 1 cut(s) 136
LpnPI CCDG 8 cut(s) 105, 119, 146, 159, 174, 210, 231, 337
Lsp1109I GCAGC 1 cut(s) 543
LweI GCATC 1 cut(s) 424
MaeI CTAG 1 cut(s) 423
MaeIII GTNAC 2 cut(s) 128, 413
MboII GAAGA 2 cut(s) 16, 254
MlsI TGGCCA 1 cut(s) 395
MluCI AATT 2 cut(s) 366, 523
MluNI TGGCCA 1 cut(s) 395
MlyI GAGTC 2 cut(s) 32, 410
MnlI CCTC 6 cut(s) 95, 233, 235, 335, 465, 493
Mox20I TGGCCA 1 cut(s) 395
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 1 cut(s) 395
MseI TTAA 1 cut(s) 363
MslI CAYNNNNRTG 2 cut(s) 271, 401
Msp20I TGGCCA 1 cut(s) 395
MspI CCGG 2 cut(s) 161, 324
MspR9I CCNGG 2 cut(s) 134, 162
MvaI CCWGG 1 cut(s) 134
MwoI GCNNNNNNNGC 1 cut(s) 488
NciI CCSGG 1 cut(s) 162
NcoI CCATGG 1 cut(s) 396
NlaIII CATG 5 cut(s) 189, 400, 446, 512, 549
NlaIV GGNNCC 1 cut(s) 350
NmuCI GTSAC 1 cut(s) 413
NspI RCATGY 1 cut(s) 512
PagI TCATGA 1 cut(s) 442
PaqCI CACCTGC 1 cut(s) 236
PdmI GAANNNNTTC 1 cut(s) 8
PflMI CCANNNNNTGG 1 cut(s) 327
PkrI GCNGC 1 cut(s) 533
PleI GAGTC 2 cut(s) 31, 410
PpsI GAGTC 2 cut(s) 31, 410
Psp6I CCWGG 1 cut(s) 132
PspGI CCWGG 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 350
RsaI GTAC 2 cut(s) 173, 332
RsaNI GTAC 2 cut(s) 172, 331
RseI CAYNNNNRTG 2 cut(s) 271, 401
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 1 cut(s) 532
SchI GAGTC 2 cut(s) 32, 410
ScrFI CCNGG 2 cut(s) 134, 162
SetI ASST 9 cut(s) 11, 73, 182, 225, 250, 350, 453, 457, 536
SfaNI GCATC 1 cut(s) 424
SmiMI CAYNNNNRTG 2 cut(s) 271, 401
Sse9I AATT 2 cut(s) 366, 523
SspMI CTAG 1 cut(s) 423
StyD4I CCNGG 2 cut(s) 132, 160
StyI CCWWGG 2 cut(s) 396, 486
TaaI ACNGT 2 cut(s) 152, 236
TaqI TCGA 1 cut(s) 240
TasI AATT 2 cut(s) 366, 523
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TscAI CASTG 2 cut(s) 204, 391
TseFI GTSAC 1 cut(s) 413
TseI GCWGC 1 cut(s) 531
Tsp45I GTSAC 1 cut(s) 413
TspDTI ATGAA 2 cut(s) 17, 423
TspRI CASTG 2 cut(s) 204, 391
Van91I CCANNNNNTGG 1 cut(s) 327
XceI RCATGY 1 cut(s) 512
XmiI GTMKAC 1 cut(s) 157
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.