RLG00000028215

Ripening-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0018475
Physical Location & Seq
Reverse (-)
20194624 .. 20201975
7352 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028215

Sequence Viewer

Length: 1206 bp
ATGTCGGAAGTGGACCGGAAATTGAGTTTGAAGAAAGGTACAAGTTCTGCAAATTTGAAAGACTTCCGATCGATGTCGGAAGTGGACCGGAAATTGAGTTTGAATAAAGGTACAAGTTCTGCAAATTTGAAAGACTTCCGATCGATGTCGGAAGTGGACCGGAAATTGAGTTTGAATAAAGGTACAAGTTCTGCAAATTTGAAAGACTTCCAATCGATGTCGGAAGTGGACCGGAAATTGAGTTCTCAGAAAGGTCCAGGGTCTGTAAATTCGAAAGATTTCCGATCGATGTCGGAAGTGGACCGGAAATTGAGTTCCAAGAAAGGTTCAAGTTTTGCAAATTTGAAAGATTTCCGATCGATGTCGGAAGTGGACCGGAAATTGAGTTCCAACAAAGGTTCATGGTCCTCAAATGTGAAAGATTTCCGATTGATATCGGAAACGAAGCTCGATGATGTTACCTGTTTGGTTGTTGCAGACTATGCCATTCCAGTTGCAGCAGTCTTTGCCAACCCAAGAATGAAGCGCAAGCAAAGGATCTTGAAGACCTTGCTTGAATTCAAGGAGGGCCTTCCTCTCTTCCTCAATGCAAGCCACATTGGACCTCTCATCATTGACACGATTTGCTTTGTTAGTGAAGCTCAAAAATGTCGTCCAAGTGGAATGATAAGAGGAAAGAAGTCCCCTTCTGGACAATGTAACCAGGAGCAAGACCCTGACTGTTGTAGACCGGGAAAAATGTACCCAACCTACTCGTGCTCACCCCCAGTGTCGAGGAACACCCACGCCTACCTCAGTCTCAACAGTTTCGAGGCAGGTGGTGAGGGCAGTCTTCCATCAGAATGTGATAACAAATACCACAATGGCAGCACTCCAATCGTAGCACTCTCCACTGGATGGTACAAGAATGGAGGAAGGTGCCATAACAACATTAAAATTACTGCTAATGGTCGCAGTGTGGTGGCCATGGTGGTGGATGAATGTGACTCTACTAGGGGATGTGATGCAGATCATGACTACCTACCACCTTGTGCTACCAACATTATTGTTGCCTCCAAGGCTGTTTGGGAAGGCTTGCATGTGTCTATAGAAAACTTGGGGCAGCTTAGATATCACATGGAACGACGCCTAATTAGTTACATTAGTAAATGTTTAGCTTGTTTGGCTTTGTATTTTGTGTTTATGTATGTATCCTGTGTGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

402

Amino Acids

44.38

Weight (kDa)

9.18

Isoelectric Point (pI)

50.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KWL1 PF24300 207 - 364 5e-51 Kiwellin-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018475)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g13961
rosa_chinensis RchiOBHm_Chr1g0354461
rosa_laevigata RLG00000009352 RLG00000028215
rosa_multiflora Rmu_sc0002737.1_g000007
rosa_roxburghii Rroxscaffold_4G00301020
rosa_rugosa Rorug01G0243100
rosa_samantha Rh1AG253700 Rh1DG252600
rosa_wichuraiana Rw1G022210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 806
Acc36I ACCTGC 1 cut(s) 806
AccB1I GGYRCC 1 cut(s) 918
AccB7I CCANNNNNTGG 1 cut(s) 897
AccI GTMKAC 1 cut(s) 727
AclWI GGATC 1 cut(s) 545
AcoI YGGCCR 1 cut(s) 963
AcsI RAATTY 6 cut(s) 52, 124, 196, 268, 340, 557
AcyI GRCGYC 1 cut(s) 1126
AdeI CACNNNGTG 1 cut(s) 1031
AfaI GTAC 5 cut(s) 40, 112, 184, 743, 902
AfiI CCNNNNNNNGG 1 cut(s) 897
AjnI CCWGG 2 cut(s) 256, 702
AloI GAACNNNNNNTCC 6 cut(s) 226, 258, 298, 330, 370, 402
AluBI AGCT 4 cut(s) 448, 641, 1105, 1157
AluI AGCT 4 cut(s) 448, 641, 1105, 1157
Alw21I GWGCWC 1 cut(s) 761
Alw26I GTCTC 1 cut(s) 803
AlwI GGATC 1 cut(s) 545
AlwNI CAGNNNCTG 1 cut(s) 263
AoxI GGCC 2 cut(s) 568, 963
ApeKI GCWGC 3 cut(s) 497, 867, 1102
ApoI RAATTY 6 cut(s) 52, 124, 196, 268, 340, 557
ArsI GACNNNNNNTTYG 2 cut(s) 637, 669
Asp700I GAANNNNTTC 6 cut(s) 62, 134, 206, 278, 350, 422
AspLEI GCGC 1 cut(s) 528
AsuC2I CCSGG 1 cut(s) 732
AsuHPI GGTGA 2 cut(s) 753, 833
AsuII TTCGAA 1 cut(s) 272
AvaII GGWCC 9 cut(s) 13, 85, 157, 229, 254, 301, 373, 405, 602
BalI TGGCCA 1 cut(s) 965
BanI GGYRCC 1 cut(s) 918
BauI CACGAG 1 cut(s) 754
BbsI GAAGAC 2 cut(s) 551, 824
Bbv12I GWGCWC 1 cut(s) 761
BbvI GCAGC 3 cut(s) 509, 879, 1114
BccI CCATC 2 cut(s) 844, 891
BcgI CGANNNNNNTGC 2 cut(s) 859, 893
BciT130I CCWGG 2 cut(s) 258, 704
BciVI GTATCC 1 cut(s) 1201
BcnI CCSGG 1 cut(s) 732
BcoDI GTCTC 1 cut(s) 803
BfaI CTAG 1 cut(s) 993
BfmI CTRYAG 1 cut(s) 1086
BfuAI ACCTGC 1 cut(s) 806
BfuI GTATCC 1 cut(s) 1201
BglI GCCNNNNNGGC 1 cut(s) 1058
BisI GCNGC 3 cut(s) 498, 868, 1103
BlsI GCNGC 3 cut(s) 499, 869, 1104
Bme1390I CCNGG 3 cut(s) 258, 704, 732
Bme18I GGWCC 9 cut(s) 13, 85, 157, 229, 254, 301, 373, 405, 602
BmiI GGNNCC 1 cut(s) 920
BmrFI CCNGG 3 cut(s) 258, 704, 732
BmrI ACTGGG 1 cut(s) 761
BmsI GCATC 1 cut(s) 994
BmuI ACTGGG 1 cut(s) 761
BpiI GAAGAC 2 cut(s) 551, 824
Bpu14I TTCGAA 1 cut(s) 272
BpuMI CCSGG 1 cut(s) 732
Bsa29I ATCGAT 5 cut(s) 71, 143, 215, 287, 359
BsaBI GATNNNNATC 2 cut(s) 433, 1008
BsaHI GRCGYC 1 cut(s) 1126
BsaJI CCNNGG 3 cut(s) 257, 966, 1056
BsaWI WCCGGW 6 cut(s) 15, 87, 159, 231, 303, 375
Bsc4I CCNNNNNNNGG 1 cut(s) 897
Bse1I ACTGG 3 cut(s) 491, 767, 898
Bse8I GATNNNNATC 2 cut(s) 433, 1008
BseBI CCWGG 2 cut(s) 258, 704
BseCI ATCGAT 5 cut(s) 71, 143, 215, 287, 359
BseDI CCNNGG 3 cut(s) 257, 966, 1056
BseGI GGATG 3 cut(s) 902, 982, 1004
BseJI GATNNNNATC 2 cut(s) 433, 1008
BseLI CCNNNNNNNGG 1 cut(s) 897
BseMII CTCAG 2 cut(s) 260, 808
BseNI ACTGG 3 cut(s) 491, 767, 898
BseXI GCAGC 3 cut(s) 509, 879, 1114
Bsh1285I CGRYCG 4 cut(s) 71, 143, 287, 359
BshFI GGCC 2 cut(s) 570, 965
BshNI GGYRCC 1 cut(s) 918
BshVI ATCGAT 5 cut(s) 71, 143, 215, 287, 359
BsiEI CGRYCG 4 cut(s) 71, 143, 287, 359
BsiHKAI GWGCWC 1 cut(s) 761
BsiSI CCGG 7 cut(s) 16, 88, 160, 232, 304, 376, 731
BslFI GGGAC 1 cut(s) 667
BslI CCNNNNNNNGG 1 cut(s) 897
BsmAI GTCTC 1 cut(s) 803
BsmFI GGGAC 1 cut(s) 667
BsnI GGCC 2 cut(s) 570, 965
Bsp119I TTCGAA 1 cut(s) 272
Bsp1286I GDGCHC 1 cut(s) 761
Bsp143I GATC 6 cut(s) 68, 140, 284, 356, 537, 1009
Bsp19I CCATGG 1 cut(s) 966
BspANI GGCC 2 cut(s) 570, 965
BspCNI CTCAG 2 cut(s) 259, 807
BspDI ATCGAT 5 cut(s) 71, 143, 215, 287, 359
BspHI TCATGA 1 cut(s) 1012
BspLI GGNNCC 1 cut(s) 920
BspMI ACCTGC 1 cut(s) 806
BspPI GGATC 1 cut(s) 545
BspT104I TTCGAA 1 cut(s) 272
BspT107I GGYRCC 1 cut(s) 918
BsrI ACTGG 3 cut(s) 491, 767, 898
BssECI CCNNGG 3 cut(s) 257, 966, 1056
BssMI GATC 6 cut(s) 68, 140, 284, 356, 537, 1009
BssNI GRCGYC 1 cut(s) 1126
BssSI CACGAG 1 cut(s) 754
BssT1I CCWWGG 2 cut(s) 966, 1056
Bst2BI CACGAG 1 cut(s) 754
Bst2UI CCWGG 2 cut(s) 258, 704
Bst4CI ACNGT 2 cut(s) 722, 806
Bst6I CTCTTC 1 cut(s) 584
BstACI GRCGYC 1 cut(s) 1126
BstAPI GCANNNNNTGC 2 cut(s) 482, 506
BstBI TTCGAA 1 cut(s) 272
BstC8I GCNNGC 3 cut(s) 530, 592, 1076
BstDEI CTNAG 3 cut(s) 246, 794, 1106
BstDSI CCRYGG 1 cut(s) 966
BstF5I GGATG 3 cut(s) 902, 982, 1004
BstHHI GCGC 1 cut(s) 528
BstKTI GATC 6 cut(s) 71, 143, 287, 359, 540, 1012
BstMAI GTCTC 1 cut(s) 803
BstMBI GATC 6 cut(s) 68, 140, 284, 356, 537, 1009
BstMCI CGRYCG 4 cut(s) 71, 143, 287, 359
BstMWI GCNNNNNNNGC 4 cut(s) 482, 506, 1058, 1163
BstNI CCWGG 2 cut(s) 258, 704
BstNSI RCATGY 1 cut(s) 1082
BstSCI CCNGG 3 cut(s) 256, 702, 730
BstSFI CTRYAG 1 cut(s) 1086
BstV1I GCAGC 3 cut(s) 509, 879, 1114
BstV2I GAAGAC 2 cut(s) 551, 824
BstX2I RGATCY 1 cut(s) 537
BstXI CCANNNNNNTGG 1 cut(s) 973
BstYI RGATCY 1 cut(s) 537
Bsu15I ATCGAT 5 cut(s) 71, 143, 215, 287, 359
BsuI GTATCC 1 cut(s) 1201
BsuRI GGCC 2 cut(s) 570, 965
BsuTUI ATCGAT 5 cut(s) 71, 143, 215, 287, 359
BtgI CCRYGG 1 cut(s) 966
BtsCI GGATG 3 cut(s) 902, 982, 1004
BtsI GCAGTG 1 cut(s) 961
BtsIMutI CAGTG 3 cut(s) 774, 891, 961
BveI ACCTGC 1 cut(s) 806
Cac8I GCNNGC 3 cut(s) 530, 592, 1076
CaiI CAGNNNCTG 1 cut(s) 263
CciI TCATGA 1 cut(s) 1012
CfoI GCGC 1 cut(s) 528
ClaI ATCGAT 5 cut(s) 71, 143, 215, 287, 359
CseI GACGC 1 cut(s) 1134
Csp6I GTAC 5 cut(s) 39, 111, 183, 742, 901
CviAII CATG 5 cut(s) 402, 967, 1013, 1079, 1117
CviQI GTAC 5 cut(s) 39, 111, 183, 742, 901
DdeI CTNAG 3 cut(s) 246, 794, 1106
DpnI GATC 6 cut(s) 70, 142, 286, 358, 539, 1011
DpnII GATC 6 cut(s) 68, 140, 284, 356, 537, 1009
DraIII CACNNNGTG 1 cut(s) 1031
EaeI YGGCCR 1 cut(s) 963
Eam1104I CTCTTC 1 cut(s) 584
EarI CTCTTC 1 cut(s) 584
Eco130I CCWWGG 2 cut(s) 966, 1056
Eco32I GATATC 2 cut(s) 435, 1112
Eco47I GGWCC 9 cut(s) 13, 85, 157, 229, 254, 301, 373, 405, 602
EcoO109I RGGNCCY 1 cut(s) 568
EcoRI GAATTC 1 cut(s) 557
EcoRII CCWGG 2 cut(s) 256, 702
EcoRV GATATC 2 cut(s) 435, 1112
EcoT14I CCWWGG 2 cut(s) 966, 1056
ErhI CCWWGG 2 cut(s) 966, 1056
FaeI CATG 5 cut(s) 405, 970, 1016, 1082, 1120
FaqI GGGAC 1 cut(s) 667
FatI CATG 5 cut(s) 401, 966, 1012, 1078, 1116
FblI GTMKAC 1 cut(s) 727
Fnu4HI GCNGC 3 cut(s) 498, 868, 1103
FokI GGATG 3 cut(s) 909, 989, 1011
Fsp4HI GCNGC 3 cut(s) 498, 868, 1103
FspBI CTAG 1 cut(s) 993
GlaI GCGC 1 cut(s) 527
GluI GCNGC 3 cut(s) 498, 868, 1103
HaeIII GGCC 2 cut(s) 570, 965
HapII CCGG 7 cut(s) 16, 88, 160, 232, 304, 376, 731
HgaI GACGC 1 cut(s) 1134
HhaI GCGC 1 cut(s) 528
Hin1I GRCGYC 1 cut(s) 1126
Hin1II CATG 5 cut(s) 405, 970, 1016, 1082, 1120
Hin6I GCGC 1 cut(s) 526
HinP1I GCGC 1 cut(s) 526
HinfI GANTC 1 cut(s) 986
HpaII CCGG 7 cut(s) 16, 88, 160, 232, 304, 376, 731
HphI GGTGA 2 cut(s) 753, 833
Hpy166II GTNNAC 7 cut(s) 13, 85, 157, 229, 301, 373, 728
Hpy188III TCNNGA 3 cut(s) 541, 690, 1013
Hpy8I GTNNAC 7 cut(s) 13, 85, 157, 229, 301, 373, 728
Hpy99I CGWCG 1 cut(s) 1128
HpyAV CCTTC 4 cut(s) 581, 696, 909, 1064
HpyCH4III ACNGT 2 cut(s) 722, 806
HpyCH4V TGCA 9 cut(s) 50, 122, 194, 338, 476, 497, 590, 1007, 1078
HpyF10VI GCNNNNNNNGC 4 cut(s) 482, 506, 1058, 1163
HpyF3I CTNAG 3 cut(s) 246, 794, 1106
Hsp92I GRCGYC 1 cut(s) 1126
Hsp92II CATG 5 cut(s) 405, 970, 1016, 1082, 1120
HspAI GCGC 1 cut(s) 526
Kzo9I GATC 6 cut(s) 68, 140, 284, 356, 537, 1009
LmnI GCTCC 1 cut(s) 706
Lsp1109I GCAGC 3 cut(s) 509, 879, 1114
LweI GCATC 1 cut(s) 994
MaeI CTAG 1 cut(s) 993
MaeIII GTNAC 4 cut(s) 457, 698, 983, 1136
MalI GATC 6 cut(s) 70, 142, 286, 358, 539, 1011
MboI GATC 6 cut(s) 68, 140, 284, 356, 537, 1009
MboII GAAGA 4 cut(s) 43, 556, 571, 824
MflI RGATCY 1 cut(s) 537
MhlI GDGCHC 1 cut(s) 761
MlsI TGGCCA 1 cut(s) 965
MluNI TGGCCA 1 cut(s) 965
MlyI GAGTC 1 cut(s) 980
MmeI TCCRAC 6 cut(s) 57, 129, 201, 273, 345, 414
Mox20I TGGCCA 1 cut(s) 965
MroXI GAANNNNTTC 6 cut(s) 62, 134, 206, 278, 350, 422
MscI TGGCCA 1 cut(s) 965
MseI TTAA 1 cut(s) 933
MslI CAYNNNNRTG 2 cut(s) 841, 971
Msp20I TGGCCA 1 cut(s) 965
MspI CCGG 7 cut(s) 16, 88, 160, 232, 304, 376, 731
MspR9I CCNGG 3 cut(s) 258, 704, 732
MvaI CCWGG 2 cut(s) 258, 704
MwoI GCNNNNNNNGC 4 cut(s) 482, 506, 1058, 1163
NciI CCSGG 1 cut(s) 732
NcoI CCATGG 1 cut(s) 966
NdeII GATC 6 cut(s) 68, 140, 284, 356, 537, 1009
NlaIII CATG 5 cut(s) 405, 970, 1016, 1082, 1120
NlaIV GGNNCC 1 cut(s) 920
NmuCI GTSAC 1 cut(s) 983
NspI RCATGY 1 cut(s) 1082
NspV TTCGAA 1 cut(s) 272
PagI TCATGA 1 cut(s) 1012
PaqCI CACCTGC 1 cut(s) 806
PdmI GAANNNNTTC 6 cut(s) 62, 134, 206, 278, 350, 422
PflMI CCANNNNNTGG 1 cut(s) 897
PkrI GCNGC 3 cut(s) 499, 869, 1104
Ple19I CGATCG 4 cut(s) 71, 143, 287, 359
PleI GAGTC 1 cut(s) 980
PpsI GAGTC 1 cut(s) 980
Psp6I CCWGG 2 cut(s) 256, 702
PspGI CCWGG 2 cut(s) 256, 702
PspN4I GGNNCC 1 cut(s) 920
PstNI CAGNNNCTG 1 cut(s) 263
PsuI RGATCY 1 cut(s) 537
PvuI CGATCG 4 cut(s) 71, 143, 287, 359
RsaI GTAC 5 cut(s) 40, 112, 184, 743, 902
RsaNI GTAC 5 cut(s) 39, 111, 183, 742, 901
RseI CAYNNNNRTG 2 cut(s) 841, 971
SaqAI TTAA 1 cut(s) 933
SatI GCNGC 3 cut(s) 498, 868, 1103
Sau3AI GATC 6 cut(s) 68, 140, 284, 356, 537, 1009
SchI GAGTC 1 cut(s) 980
ScrFI CCNGG 3 cut(s) 258, 704, 732
SduI GDGCHC 1 cut(s) 761
SfaNI GCATC 1 cut(s) 994
SfcI CTRYAG 1 cut(s) 1086
SfuI TTCGAA 1 cut(s) 272
SinI GGWCC 9 cut(s) 13, 85, 157, 229, 254, 301, 373, 405, 602
SmiMI CAYNNNNRTG 2 cut(s) 841, 971
SspMI CTAG 1 cut(s) 993
StyD4I CCNGG 3 cut(s) 256, 702, 730
StyI CCWWGG 2 cut(s) 966, 1056
TaaI ACNGT 2 cut(s) 722, 806
TaqI TCGA 9 cut(s) 71, 143, 215, 272, 287, 359, 450, 773, 810
Tru1I TTAA 1 cut(s) 933
Tru9I TTAA 1 cut(s) 933
TscAI CASTG 3 cut(s) 774, 898, 961
TseFI GTSAC 1 cut(s) 983
TseI GCWGC 3 cut(s) 497, 867, 1102
Tsp45I GTSAC 1 cut(s) 983
TspDTI ATGAA 3 cut(s) 390, 536, 993
TspRI CASTG 3 cut(s) 774, 898, 961
Van91I CCANNNNNTGG 1 cut(s) 897
VpaK11BI GGWCC 9 cut(s) 13, 85, 157, 229, 254, 301, 373, 405, 602
XapI RAATTY 6 cut(s) 52, 124, 196, 268, 340, 557
XceI RCATGY 1 cut(s) 1082
XmiI GTMKAC 1 cut(s) 727
XmnI GAANNNNTTC 6 cut(s) 62, 134, 206, 278, 350, 422
XspI CTAG 1 cut(s) 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.