RLG00000012357

Cell division control protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0018656
Physical Location & Seq
Reverse (-)
17837342 .. 17840826
3485 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012357

Sequence Viewer

Length: 846 bp
ATGGCTTTTCCATCAGCGTTGGAGTCCAGATTTCATGATGTGGAGCTCATCGGCCAAGGTCCTCGGTTCATTGTCTATGGAGCGAAGCTGAAGAATACCGCCATCAGAGTTGCTGTGAAGGTGATGAGGCCAGAGGTGCTTGCGTGTCTCGAGCAGGGTCGGAGTGCCATCTCTTCCGCTCTCCAGTCCGAGCTGCTGAAATCCTCGTCGGCTGCGTACGTGAGGCTTTACTTGTACGATTACGGCAGCCACGGCGCTCCATACTTTGTGTATGACCTGATGGTGGAAGAGAATCTCACTGGCAAAGGAGAGCCCCGAGAGCCGGCTTTGGTGAAGCAGGTGATGCGCGAGCTTTGCCAGGAGCTCGCCGGAATTCACTCCGGCGGCGGAACCCAAGGTGCACTCCACCCAAACAACTTGTTCCTGAAGGATGGACATCTGTTCATCGGAGATTACGGGTTCGACGGAGCTTTCCCTGTTCCAGCTACTGCAGCAACGCCATATTCCTACTCCGTCGTCCGCTACAAGGCTATGGAGAAACAGAGGACGGCAAGCGGTGATATTTGGGCGGCGGGTTGTGTTTTCGCCGAGCTGGCCACTGGAAACCCTCTCTTCACTTCAGGGCCAATTTCATCGCAGAAAGAACGGATTTACCGGATTCTTGGAAGGAGGGCCGGCACCGGCGACATCACGGACTCTGACATCGAGTCCCGTAAGATGCAGTTGGCGGCTGCTGTTCCCCAGCTTTCACAGGAAGGGATCGACCTCCTTTATGAGATGCTGCAAGAGCATCCCGTCGACCGGATTGGTGCGGAGGCAGCACTAGACCATCCTTTTTTTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000307 GO:0000902 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008284 GO:0009058 GO:0009059 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010033 GO:0010103 GO:0010154 GO:0010374 GO:0010376 GO:0010389 GO:0010440 GO:0010444 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0022414 GO:0023052 GO:0030154 GO:0030332 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0032991 GO:0034641 GO:0034645 GO:0036211 GO:0040007 GO:0042023 GO:0042127 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044770 GO:0044772 GO:0044786 GO:0044843 GO:0046483 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051239 GO:0051716 GO:0051726 GO:0060255 GO:0060560 GO:0061458 GO:0061695 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097472 GO:0099402 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901987 GO:1901990 GO:1902494 GO:1902554 GO:1902749 GO:1902806 GO:1902911 GO:1903047 GO:1990234 GO:2000026 GO:2000037 GO:2000112
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

282

Amino Acids

30.64

Weight (kDa)

5.83

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 11 - 280 6.9e-22 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018656)

Species Orthologous Gene IDs
rosa_laevigata RLG00000012355 RLG00000012357
rosa_roxburghii Rroxscaffold_7G00179800
rosa_rugosa Rorug06G0200200 Rorug06G0200200 Rorug06G0200300
rosa_samantha Rh6AG310300 Rh6BG313600 Rh6BG316200 Rh6BG316500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 328
Acc36I ACCTGC 1 cut(s) 328
AccB1I GGYRCC 1 cut(s) 677
AccBSI CCGCTC 1 cut(s) 179
AccI GTMKAC 1 cut(s) 798
AccII CGCG 1 cut(s) 348
AclWI GGATC 1 cut(s) 767
AcoI YGGCCR 2 cut(s) 52, 594
AcsI RAATTY 1 cut(s) 372
AcuI CTGAAG 3 cut(s) 110, 446, 603
AfaI GTAC 2 cut(s) 218, 236
AfiI CCNNNNNNNGG 5 cut(s) 283, 322, 526, 801, 839
AhdI GACNNNNNGTC 1 cut(s) 706
AjnI CCWGG 1 cut(s) 357
AluBI AGCT 9 cut(s) 46, 88, 193, 352, 364, 470, 485, 592, 745
AluI AGCT 9 cut(s) 46, 88, 193, 352, 364, 470, 485, 592, 745
Alw21I GWGCWC 3 cut(s) 48, 366, 403
Alw26I GTCTC 1 cut(s) 152
Alw44I GTGCAC 1 cut(s) 399
AlwI GGATC 1 cut(s) 767
AlwNI CAGNNNCTG 1 cut(s) 488
Ama87I CYCGRG 2 cut(s) 149, 315
AoxI GGCC 5 cut(s) 52, 128, 594, 623, 672
ApaLI GTGCAC 1 cut(s) 399
ApeKI GCWGC 7 cut(s) 193, 212, 246, 491, 731, 781, 818
ApoI RAATTY 1 cut(s) 372
AspLEI GCGC 2 cut(s) 257, 348
AspS9I GGNCC 3 cut(s) 59, 623, 672
AsuHPI GGTGA 4 cut(s) 133, 343, 352, 569
AvaI CYCGRG 2 cut(s) 149, 315
AvaII GGWCC 1 cut(s) 59
BaeGI GKGCMC 1 cut(s) 403
BalI TGGCCA 1 cut(s) 596
BanI GGYRCC 1 cut(s) 677
BanII GRGCYC 3 cut(s) 48, 315, 366
Bbv12I GWGCWC 3 cut(s) 48, 366, 403
BbvI GCAGC 7 cut(s) 180, 199, 258, 503, 718, 768, 830
BccI CCATC 6 cut(s) 19, 110, 176, 274, 425, 837
BceAI ACGGC 3 cut(s) 259, 268, 564
BciT130I CCWGG 1 cut(s) 359
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 1 cut(s) 824
BfmI CTRYAG 1 cut(s) 489
BfoI RGCGCY 1 cut(s) 258
BfuAI ACCTGC 1 cut(s) 328
BglI GCCNNNNNGGC 1 cut(s) 593
Bme1390I CCNGG 1 cut(s) 359
Bme18I GGWCC 1 cut(s) 59
BmeRI GACNNNNNGTC 1 cut(s) 706
BmeT110I CYCGRG 2 cut(s) 149, 315
BmgT120I GGNCC 3 cut(s) 59, 623, 672
BmiI GGNNCC 2 cut(s) 391, 679
BmrFI CCNGG 1 cut(s) 359
BmsI GCATC 4 cut(s) 333, 708, 768, 799
BpmI CTGGAG 1 cut(s) 167
BsaAI YACGTR 1 cut(s) 220
BsaBI GATNNNNATC 1 cut(s) 435
BsaJI CCNNGG 4 cut(s) 55, 62, 250, 394
BsaWI WCCGGW 2 cut(s) 654, 801
Bsc4I CCNNNNNNNGG 5 cut(s) 283, 322, 526, 801, 839
Bse118I RCCGGY 3 cut(s) 322, 674, 680
Bse1I ACTGG 3 cut(s) 184, 304, 604
Bse8I GATNNNNATC 1 cut(s) 435
BseBI CCWGG 1 cut(s) 359
BseDI CCNNGG 4 cut(s) 55, 62, 250, 394
BseGI GGATG 3 cut(s) 436, 790, 829
BseJI GATNNNNATC 1 cut(s) 435
BseLI CCNNNNNNNGG 5 cut(s) 283, 322, 526, 801, 839
BseNI ACTGG 3 cut(s) 184, 304, 604
BseSI GKGCMC 1 cut(s) 403
BseXI GCAGC 7 cut(s) 180, 199, 258, 503, 718, 768, 830
BseYI CCCAGC 1 cut(s) 741
Bsh1236I CGCG 1 cut(s) 348
Bsh1285I CGRYCG 1 cut(s) 802
BshFI GGCC 5 cut(s) 54, 130, 596, 625, 674
BshNI GGYRCC 1 cut(s) 677
BsiEI CGRYCG 1 cut(s) 802
BsiHKAI GWGCWC 3 cut(s) 48, 366, 403
BsiHKCI CYCGRG 2 cut(s) 149, 315
BsiSI CCGG 7 cut(s) 323, 369, 381, 655, 675, 681, 802
BsiWI CGTACG 1 cut(s) 216
BslFI GGGAC 1 cut(s) 694
BslI CCNNNNNNNGG 5 cut(s) 283, 322, 526, 801, 839
BsmAI GTCTC 1 cut(s) 152
BsmFI GGGAC 1 cut(s) 694
BsnI GGCC 5 cut(s) 54, 130, 596, 625, 674
BsoBI CYCGRG 2 cut(s) 149, 315
Bsp1286I GDGCHC 4 cut(s) 48, 315, 366, 403
Bsp143I GATC 1 cut(s) 759
BspANI GGCC 5 cut(s) 54, 130, 596, 625, 674
BspFNI CGCG 1 cut(s) 348
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 2 cut(s) 391, 679
BspMAI CTGCAG 1 cut(s) 493
BspMI ACCTGC 1 cut(s) 328
BspPI GGATC 1 cut(s) 767
BspT107I GGYRCC 1 cut(s) 677
BsrBI CCGCTC 1 cut(s) 179
BsrFI RCCGGY 3 cut(s) 322, 674, 680
BsrI ACTGG 3 cut(s) 184, 304, 604
BssAI RCCGGY 3 cut(s) 322, 674, 680
BssECI CCNNGG 4 cut(s) 55, 62, 250, 394
BssMI GATC 1 cut(s) 759
BssT1I CCWWGG 2 cut(s) 55, 394
Bst2UI CCWGG 1 cut(s) 359
Bst6I CTCTTC 3 cut(s) 178, 282, 617
BstAPI GCANNNNNTGC 1 cut(s) 343
BstBAI YACGTR 1 cut(s) 220
BstC8I GCNNGC 7 cut(s) 141, 324, 350, 366, 553, 594, 676
BstDSI CCRYGG 1 cut(s) 250
BstF5I GGATG 3 cut(s) 436, 790, 829
BstFNI CGCG 1 cut(s) 348
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 2 cut(s) 257, 348
BstKTI GATC 1 cut(s) 762
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 1 cut(s) 759
BstMCI CGRYCG 1 cut(s) 802
BstMWI GCNNNNNNNGC 9 cut(s) 136, 252, 319, 343, 354, 491, 593, 787, 818
BstNI CCWGG 1 cut(s) 359
BstSCI CCNGG 1 cut(s) 357
BstSFI CTRYAG 1 cut(s) 489
BstSLI GKGCMC 1 cut(s) 403
BstUI CGCG 1 cut(s) 348
BstV1I GCAGC 7 cut(s) 180, 199, 258, 503, 718, 768, 830
BsuRI GGCC 5 cut(s) 54, 130, 596, 625, 674
BtgI CCRYGG 1 cut(s) 250
BtgZI GCGATG 1 cut(s) 618
BtsCI GGATG 3 cut(s) 436, 790, 829
BtsIMutI CAGTG 2 cut(s) 297, 597
BveI ACCTGC 1 cut(s) 328
Cac8I GCNNGC 7 cut(s) 141, 324, 350, 366, 553, 594, 676
CaiI CAGNNNCTG 1 cut(s) 488
CciI TCATGA 1 cut(s) 34
CfoI GCGC 2 cut(s) 257, 348
Cfr10I RCCGGY 3 cut(s) 322, 674, 680
Cfr13I GGNCC 3 cut(s) 59, 623, 672
Csp6I GTAC 2 cut(s) 217, 235
CviAII CATG 1 cut(s) 35
CviQI GTAC 2 cut(s) 217, 235
DpnI GATC 1 cut(s) 761
DpnII GATC 1 cut(s) 759
DriI GACNNNNNGTC 1 cut(s) 706
EaeI YGGCCR 2 cut(s) 52, 594
Eam1104I CTCTTC 3 cut(s) 178, 282, 617
Eam1105I GACNNNNNGTC 1 cut(s) 706
EarI CTCTTC 3 cut(s) 178, 282, 617
EciI GGCGGA 1 cut(s) 402
Ecl136II GAGCTC 2 cut(s) 46, 364
Eco130I CCWWGG 2 cut(s) 55, 394
Eco24I GRGCYC 3 cut(s) 48, 315, 366
Eco47I GGWCC 1 cut(s) 59
Eco53kI GAGCTC 2 cut(s) 46, 364
Eco57I CTGAAG 3 cut(s) 110, 446, 603
Eco88I CYCGRG 2 cut(s) 149, 315
EcoICRI GAGCTC 2 cut(s) 46, 364
EcoO109I RGGNCCY 1 cut(s) 59
EcoRI GAATTC 1 cut(s) 372
EcoRII CCWGG 1 cut(s) 357
EcoT14I CCWWGG 2 cut(s) 55, 394
EcoT38I GRGCYC 3 cut(s) 48, 315, 366
ErhI CCWWGG 2 cut(s) 55, 394
FaeI CATG 1 cut(s) 38
FaiI YATR 7 cut(s) 36, 78, 262, 273, 502, 533, 774
FaqI GGGAC 1 cut(s) 694
FatI CATG 1 cut(s) 34
FauI CCCGC 1 cut(s) 565
FblI GTMKAC 1 cut(s) 798
FokI GGATG 3 cut(s) 443, 777, 816
FriOI GRGCYC 3 cut(s) 48, 315, 366
FspBI CTAG 1 cut(s) 824
GlaI GCGC 2 cut(s) 256, 347
GsaI CCCAGC 1 cut(s) 745
GsuI CTGGAG 1 cut(s) 167
HaeII RGCGCY 1 cut(s) 258
HaeIII GGCC 5 cut(s) 54, 130, 596, 625, 674
HapII CCGG 7 cut(s) 323, 369, 381, 655, 675, 681, 802
HhaI GCGC 2 cut(s) 257, 348
Hin1II CATG 1 cut(s) 38
Hin6I GCGC 2 cut(s) 255, 346
HinP1I GCGC 2 cut(s) 255, 346
HincII GTYRAC 1 cut(s) 799
HindII GTYRAC 1 cut(s) 799
HinfI GANTC 5 cut(s) 23, 292, 658, 695, 707
HpaII CCGG 7 cut(s) 323, 369, 381, 655, 675, 681, 802
HphI GGTGA 4 cut(s) 133, 343, 352, 569
Hpy166II GTNNAC 2 cut(s) 401, 799
Hpy188I TCNGA 5 cut(s) 107, 162, 190, 449, 700
Hpy188III TCNNGA 4 cut(s) 27, 35, 149, 424
Hpy8I GTNNAC 2 cut(s) 401, 799
Hpy99I CGWCG 4 cut(s) 211, 467, 518, 800
HpyAV CCTTC 4 cut(s) 112, 421, 660, 749
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 4 cut(s) 401, 491, 721, 784
HpyF10VI GCNNNNNNNGC 9 cut(s) 136, 252, 319, 343, 354, 491, 593, 787, 818
HpySE526I ACGT 1 cut(s) 219
Hsp92II CATG 1 cut(s) 38
HspAI GCGC 2 cut(s) 255, 346
KroI GCCGGC 2 cut(s) 322, 674
KroNI GCCGGC 2 cut(s) 324, 676
Kzo9I GATC 1 cut(s) 759
LmnI GCTCC 5 cut(s) 43, 80, 262, 361, 467
Lsp1109I GCAGC 7 cut(s) 180, 199, 258, 503, 718, 768, 830
LweI GCATC 4 cut(s) 333, 708, 768, 799
MaeI CTAG 1 cut(s) 824
MaeII ACGT 1 cut(s) 219
MalI GATC 1 cut(s) 761
MbiI CCGCTC 1 cut(s) 179
MboI GATC 1 cut(s) 759
MboII GAAGA 4 cut(s) 103, 165, 299, 604
MhlI GDGCHC 4 cut(s) 48, 315, 366, 403
MlsI TGGCCA 1 cut(s) 596
MluCI AATT 2 cut(s) 372, 627
MluNI TGGCCA 1 cut(s) 596
MlyI GAGTC 3 cut(s) 32, 689, 716
MmeI TCCRAC 1 cut(s) 140
Mox20I TGGCCA 1 cut(s) 596
MroNI GCCGGC 2 cut(s) 322, 674
MscI TGGCCA 1 cut(s) 596
MseI TTAA 1 cut(s) 844
Msp20I TGGCCA 1 cut(s) 596
MspI CCGG 7 cut(s) 323, 369, 381, 655, 675, 681, 802
MspR9I CCNGG 1 cut(s) 359
MvaI CCWGG 1 cut(s) 359
MvnI CGCG 1 cut(s) 348
MwoI GCNNNNNNNGC 9 cut(s) 136, 252, 319, 343, 354, 491, 593, 787, 818
NaeI GCCGGC 2 cut(s) 324, 676
NdeII GATC 1 cut(s) 759
NgoMIV GCCGGC 2 cut(s) 322, 674
NlaIII CATG 1 cut(s) 38
NlaIV GGNNCC 2 cut(s) 391, 679
NmeAIII GCCGAG 1 cut(s) 613
PaeR7I CTCGAG 1 cut(s) 149
PagI TCATGA 1 cut(s) 34
PaqCI CACCTGC 1 cut(s) 328
PcsI WCGNNNNNNNCGW 1 cut(s) 212
PdiI GCCGGC 2 cut(s) 324, 676
PfeI GAWTC 2 cut(s) 292, 658
Pfl23II CGTACG 1 cut(s) 216
PleI GAGTC 3 cut(s) 31, 689, 715
PpsI GAGTC 3 cut(s) 31, 689, 715
Ppu21I YACGTR 1 cut(s) 220
PpuMI RGGWCCY 1 cut(s) 59
Psp124BI GAGCTC 2 cut(s) 48, 366
Psp5II RGGWCCY 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 357
PspFI CCCAGC 1 cut(s) 741
PspGI CCWGG 1 cut(s) 357
PspLI CGTACG 1 cut(s) 216
PspN4I GGNNCC 2 cut(s) 391, 679
PspPI GGNCC 3 cut(s) 59, 623, 672
PspPPI RGGWCCY 1 cut(s) 59
PstI CTGCAG 1 cut(s) 493
PstNI CAGNNNCTG 1 cut(s) 488
RsaI GTAC 2 cut(s) 218, 236
RsaNI GTAC 2 cut(s) 217, 235
SacI GAGCTC 2 cut(s) 48, 366
SalI GTCGAC 1 cut(s) 797
SaqAI TTAA 1 cut(s) 844
Sau3AI GATC 1 cut(s) 759
Sau96I GGNCC 3 cut(s) 59, 623, 672
SchI GAGTC 3 cut(s) 32, 689, 716
ScrFI CCNGG 1 cut(s) 359
SduI GDGCHC 4 cut(s) 48, 315, 366, 403
SfaNI GCATC 4 cut(s) 333, 708, 768, 799
SfcI CTRYAG 1 cut(s) 489
Sfr274I CTCGAG 1 cut(s) 149
SgrAI CRCCGGYG 1 cut(s) 680
SinI GGWCC 1 cut(s) 59
SlaI CTCGAG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 2 cut(s) 372, 627
SspMI CTAG 1 cut(s) 824
SstI GAGCTC 2 cut(s) 48, 366
StyD4I CCNGG 1 cut(s) 357
StyI CCWWGG 2 cut(s) 55, 394
TaiI ACGT 1 cut(s) 222
TaqI TCGA 5 cut(s) 150, 462, 705, 762, 798
TasI AATT 2 cut(s) 372, 627
TauI GCSGC 3 cut(s) 387, 572, 731
TfiI GAWTC 2 cut(s) 292, 658
Tru1I TTAA 1 cut(s) 844
Tru9I TTAA 1 cut(s) 844
TscAI CASTG 2 cut(s) 304, 604
TseI GCWGC 7 cut(s) 193, 212, 246, 491, 731, 781, 818
TspDTI ATGAA 4 cut(s) 23, 58, 433, 621
TspGWI ACGGA 4 cut(s) 480, 502, 661, 707
TspRI CASTG 2 cut(s) 304, 604
VneI GTGCAC 1 cut(s) 399
VpaK11BI GGWCC 1 cut(s) 59
XapI RAATTY 1 cut(s) 372
XcmI CCANNNNNNNNNTGG 1 cut(s) 836
XhoI CTCGAG 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 798
XspI CTAG 1 cut(s) 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.