Rh6BG316200

Cell division protein kinase. Source PGD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0018656
Physical Location & Seq
Forward (+)
54613047 .. 54616291
3245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG316200.1

Sequence Viewer

Length: 801 bp
ATGAGGCCAGAGGTGCTTGCATGTCTCGAGGGTCGGAGTGCCATCTCTTCCGCTCTCCAGTCCGAGCTGCTGAAATCCTCGTCGGCTGCCTACGTGAGGCTGTACCTCTACGACTACGGCAGCGACGGGGCTCCGTACTTTTTGTATGAGCGGATGGACGAGAACCTCACGGGAAAAGGAGAGCCCCGAGAGCCGGGTTTGGTCAAGCAGCTCATGCGCGAGCTTTGCCAGGAGCTCGCCGGAATTCACTCCAGCGGCGGAACCCATGGTGCGCTCCACCCAAACAACTTGTTCTTGAAGGATGGACATCTGTTCATCGGAGATTACGGGTTCGAAGGAGCTTTCCCTGTTCCAGCTACCGCAGCAATGCCATACGGTTACTCCGTCATCCGCTACAAGGCCTTGGAGAGAAAGAGGACGCCAAGCGGGGATATTTGGGCAGCGGGCTGTGTTTTTGCGGAGCTGGCCACTGGAAGCCCTCTCTTCACCTCAGGGCCAATTTCATCGCAGAAGGAACGGATTTACCGGATTCTTGGAAGGAGGGCTGGCACCGGCGACATCACGGACTCTGACATCGAGTCCTGTAAGATGCGTTTGGCGGCTGCTGTTCCCCAGCTTTCACAGGAAGGGATCGACCTCCTTTATGTGAGTGTGGCTAATTTGCAACAATTTGGAGATGCTGCAAGAGCATCCCGTCGACCGGATTGGTGCGGAGGCAGCACTAGAGCATCCTTTTTTTGGTTAAAAGGGACATGCTGCAAGAGCATCCCGTCGACCGGATTGGTGTGGAGGCAGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

266

Amino Acids

29.04

Weight (kDa)

8.12

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 46 - 177 8.7e-11 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018656)

Species Orthologous Gene IDs
rosa_laevigata RLG00000012355 RLG00000012357
rosa_roxburghii Rroxscaffold_7G00179800
rosa_rugosa Rorug06G0200200 Rorug06G0200200 Rorug06G0200300
rosa_samantha Rh6AG310300 Rh6BG313600 Rh6BG316200 Rh6BG316500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 548
AccBSI CCGCTC 2 cut(s) 53, 151
AccI GTMKAC 2 cut(s) 697, 773
AccII CGCG 1 cut(s) 219
AclWI GGATC 1 cut(s) 638
AcoI YGGCCR 1 cut(s) 465
AcsI RAATTY 1 cut(s) 243
AcyI GRCGYC 1 cut(s) 419
AfaI GTAC 2 cut(s) 104, 137
AfiI CCNNNNNNNGG 6 cut(s) 96, 193, 397, 700, 738, 776
AgsI TTSAA 1 cut(s) 298
AhdI GACNNNNNGTC 1 cut(s) 577
AjnI CCWGG 1 cut(s) 228
AluBI AGCT 8 cut(s) 67, 211, 223, 235, 341, 356, 463, 616
AluI AGCT 8 cut(s) 67, 211, 223, 235, 341, 356, 463, 616
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 1 cut(s) 638
Ama87I CYCGRG 2 cut(s) 26, 186
AoxI GGCC 4 cut(s) 5, 399, 465, 494
ApoI RAATTY 1 cut(s) 243
AspLEI GCGC 2 cut(s) 219, 274
AspS9I GGNCC 1 cut(s) 494
AsuC2I CCSGG 1 cut(s) 195
AsuHPI GGTGA 1 cut(s) 478
AsuII TTCGAA 1 cut(s) 333
AvaI CYCGRG 2 cut(s) 26, 186
AxyI CCTNAGG 1 cut(s) 490
BalI TGGCCA 1 cut(s) 467
BanI GGYRCC 1 cut(s) 548
BanII GRGCYC 3 cut(s) 133, 186, 237
Bbv12I GWGCWC 1 cut(s) 237
BccI CCATC 3 cut(s) 50, 148, 296
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 1 cut(s) 230
BcnI CCSGG 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 29
BfaI CTAG 2 cut(s) 723, 799
Bme1390I CCNGG 2 cut(s) 195, 230
BmeRI GACNNNNNGTC 1 cut(s) 577
BmeT110I CYCGRG 2 cut(s) 26, 186
BmgT120I GGNCC 1 cut(s) 494
BmiI GGNNCC 3 cut(s) 132, 262, 550
BmrFI CCNGG 2 cut(s) 195, 230
BmsI GCATC 5 cut(s) 579, 667, 698, 737, 774
BpmI CTGGAG 2 cut(s) 41, 235
Bpu14I TTCGAA 1 cut(s) 333
BpuMI CCSGG 1 cut(s) 195
BsaAI YACGTR 1 cut(s) 94
BsaBI GATNNNNATC 1 cut(s) 306
BsaHI GRCGYC 1 cut(s) 419
BsaJI CCNNGG 2 cut(s) 265, 402
BsaWI WCCGGW 3 cut(s) 525, 700, 776
BsaXI ACNNNNNCTCC 2 cut(s) 365, 395
Bsc4I CCNNNNNNNGG 6 cut(s) 96, 193, 397, 700, 738, 776
Bse118I RCCGGY 1 cut(s) 551
Bse1I ACTGG 2 cut(s) 58, 475
Bse21I CCTNAGG 1 cut(s) 490
Bse3DI GCAATG 1 cut(s) 372
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 1 cut(s) 230
BseDI CCNNGG 2 cut(s) 265, 402
BseGI GGATG 6 cut(s) 159, 307, 387, 689, 728, 765
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 6 cut(s) 96, 193, 397, 700, 738, 776
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 1 cut(s) 504
BseNI ACTGG 2 cut(s) 58, 475
BseYI CCCAGC 1 cut(s) 612
Bsh1236I CGCG 1 cut(s) 219
Bsh1285I CGRYCG 2 cut(s) 701, 777
BshFI GGCC 4 cut(s) 7, 401, 467, 496
BshNI GGYRCC 1 cut(s) 548
BsiEI CGRYCG 2 cut(s) 701, 777
BsiHKAI GWGCWC 1 cut(s) 237
BsiHKCI CYCGRG 2 cut(s) 26, 186
BsiSI CCGG 6 cut(s) 194, 240, 526, 552, 701, 777
BslFI GGGAC 1 cut(s) 763
BslI CCNNNNNNNGG 6 cut(s) 96, 193, 397, 700, 738, 776
BsmAI GTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 763
BsnI GGCC 4 cut(s) 7, 401, 467, 496
BsoBI CYCGRG 2 cut(s) 26, 186
Bsp119I TTCGAA 1 cut(s) 333
Bsp1286I GDGCHC 3 cut(s) 133, 186, 237
Bsp143I GATC 1 cut(s) 630
Bsp19I CCATGG 1 cut(s) 265
BspANI GGCC 4 cut(s) 7, 401, 467, 496
BspCNI CTCAG 1 cut(s) 503
BspFNI CGCG 1 cut(s) 219
BspLI GGNNCC 3 cut(s) 132, 262, 550
BspPI GGATC 1 cut(s) 638
BspT104I TTCGAA 1 cut(s) 333
BspT107I GGYRCC 1 cut(s) 548
BsrBI CCGCTC 2 cut(s) 53, 151
BsrDI GCAATG 1 cut(s) 372
BsrFI RCCGGY 1 cut(s) 551
BsrI ACTGG 2 cut(s) 58, 475
BssAI RCCGGY 1 cut(s) 551
BssECI CCNNGG 2 cut(s) 265, 402
BssMI GATC 1 cut(s) 630
BssNI GRCGYC 1 cut(s) 419
BssT1I CCWWGG 2 cut(s) 265, 402
Bst2UI CCWGG 1 cut(s) 230
Bst4CI ACNGT 1 cut(s) 377
Bst6I CTCTTC 2 cut(s) 52, 488
BstACI GRCGYC 1 cut(s) 419
BstAPI GCANNNNNTGC 1 cut(s) 214
BstBAI YACGTR 1 cut(s) 94
BstBI TTCGAA 1 cut(s) 333
BstC8I GCNNGC 6 cut(s) 18, 221, 237, 445, 465, 547
BstDEI CTNAG 1 cut(s) 490
BstDSI CCRYGG 1 cut(s) 265
BstENI CCTNNNNNAGG 1 cut(s) 94
BstF5I GGATG 6 cut(s) 159, 307, 387, 689, 728, 765
BstFNI CGCG 1 cut(s) 219
BstHHI GCGC 2 cut(s) 219, 274
BstKTI GATC 1 cut(s) 633
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 1 cut(s) 630
BstMCI CGRYCG 2 cut(s) 701, 777
BstMWI GCNNNNNNNGC 9 cut(s) 13, 190, 214, 225, 362, 464, 686, 717, 762
BstNI CCWGG 1 cut(s) 230
BstNSI RCATGY 2 cut(s) 24, 756
BstSCI CCNGG 2 cut(s) 193, 228
BstUI CGCG 1 cut(s) 219
Bsu36I CCTNAGG 1 cut(s) 490
BsuRI GGCC 4 cut(s) 7, 401, 467, 496
BtgI CCRYGG 1 cut(s) 265
BtgZI GCGATG 1 cut(s) 489
BtsCI GGATG 6 cut(s) 159, 307, 387, 689, 728, 765
BtsIMutI CAGTG 1 cut(s) 468
Cac8I GCNNGC 6 cut(s) 18, 221, 237, 445, 465, 547
CfoI GCGC 2 cut(s) 219, 274
Cfr10I RCCGGY 1 cut(s) 551
Cfr13I GGNCC 1 cut(s) 494
CseI GACGC 1 cut(s) 427
Csp6I GTAC 2 cut(s) 103, 136
CviAII CATG 4 cut(s) 21, 214, 266, 753
CviQI GTAC 2 cut(s) 103, 136
DdeI CTNAG 1 cut(s) 490
DpnI GATC 1 cut(s) 632
DpnII GATC 1 cut(s) 630
DriI GACNNNNNGTC 1 cut(s) 577
EaeI YGGCCR 1 cut(s) 465
Eam1104I CTCTTC 2 cut(s) 52, 488
Eam1105I GACNNNNNGTC 1 cut(s) 577
EarI CTCTTC 2 cut(s) 52, 488
EciI GGCGGA 1 cut(s) 273
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 2 cut(s) 265, 402
Eco147I AGGCCT 1 cut(s) 401
Eco24I GRGCYC 3 cut(s) 133, 186, 237
Eco53kI GAGCTC 1 cut(s) 235
Eco81I CCTNAGG 1 cut(s) 490
Eco88I CYCGRG 2 cut(s) 26, 186
EcoICRI GAGCTC 1 cut(s) 235
EcoNI CCTNNNNNAGG 1 cut(s) 94
EcoRI GAATTC 1 cut(s) 243
EcoRII CCWGG 1 cut(s) 228
EcoT14I CCWWGG 2 cut(s) 265, 402
EcoT38I GRGCYC 3 cut(s) 133, 186, 237
ErhI CCWWGG 2 cut(s) 265, 402
FaeI CATG 4 cut(s) 24, 217, 269, 756
FaiI YATR 7 cut(s) 22, 147, 215, 267, 373, 645, 754
FaqI GGGAC 1 cut(s) 763
FatI CATG 4 cut(s) 20, 213, 265, 752
FauI CCCGC 2 cut(s) 419, 436
FblI GTMKAC 2 cut(s) 697, 773
FokI GGATG 6 cut(s) 166, 314, 374, 676, 715, 752
FriOI GRGCYC 3 cut(s) 133, 186, 237
FspBI CTAG 2 cut(s) 723, 799
GlaI GCGC 2 cut(s) 218, 273
GsaI CCCAGC 1 cut(s) 616
GsuI CTGGAG 2 cut(s) 41, 235
HaeIII GGCC 4 cut(s) 7, 401, 467, 496
HapII CCGG 6 cut(s) 194, 240, 526, 552, 701, 777
HgaI GACGC 1 cut(s) 427
HhaI GCGC 2 cut(s) 219, 274
Hin1I GRCGYC 1 cut(s) 419
Hin1II CATG 4 cut(s) 24, 217, 269, 756
Hin6I GCGC 2 cut(s) 217, 272
HinP1I GCGC 2 cut(s) 217, 272
HincII GTYRAC 2 cut(s) 698, 774
HindII GTYRAC 2 cut(s) 698, 774
HinfI GANTC 3 cut(s) 529, 566, 578
HpaII CCGG 6 cut(s) 194, 240, 526, 552, 701, 777
HphI GGTGA 1 cut(s) 478
Hpy166II GTNNAC 2 cut(s) 698, 774
Hpy188I TCNGA 4 cut(s) 36, 64, 320, 571
Hpy188III TCNNGA 2 cut(s) 26, 295
Hpy8I GTNNAC 2 cut(s) 698, 774
Hpy99I CGWCG 4 cut(s) 85, 128, 699, 775
HpyAV CCTTC 5 cut(s) 292, 329, 505, 531, 620
HpyCH4III ACNGT 1 cut(s) 377
HpyCH4IV ACGT 1 cut(s) 93
HpyCH4V TGCA 4 cut(s) 20, 664, 683, 759
HpyF10VI GCNNNNNNNGC 9 cut(s) 13, 190, 214, 225, 362, 464, 686, 717, 762
HpyF3I CTNAG 1 cut(s) 490
HpySE526I ACGT 1 cut(s) 93
Hsp92I GRCGYC 1 cut(s) 419
Hsp92II CATG 4 cut(s) 24, 217, 269, 756
HspAI GCGC 2 cut(s) 217, 272
Kzo9I GATC 1 cut(s) 630
LmnI GCTCC 5 cut(s) 136, 232, 279, 338, 460
LweI GCATC 5 cut(s) 579, 667, 698, 737, 774
MaeI CTAG 2 cut(s) 723, 799
MaeII ACGT 1 cut(s) 93
MaeIII GTNAC 1 cut(s) 377
MalI GATC 1 cut(s) 632
MbiI CCGCTC 2 cut(s) 53, 151
MboI GATC 1 cut(s) 630
MboII GAAGA 2 cut(s) 39, 475
MhlI GDGCHC 3 cut(s) 133, 186, 237
MlsI TGGCCA 1 cut(s) 467
MluCI AATT 4 cut(s) 243, 498, 658, 668
MluNI TGGCCA 1 cut(s) 467
MlyI GAGTC 2 cut(s) 560, 587
MmeI TCCRAC 1 cut(s) 14
Mox20I TGGCCA 1 cut(s) 467
MscI TGGCCA 1 cut(s) 467
MseI TTAA 1 cut(s) 743
Msp20I TGGCCA 1 cut(s) 467
MspA1I CMGCKG 2 cut(s) 255, 443
MspI CCGG 6 cut(s) 194, 240, 526, 552, 701, 777
MspR9I CCNGG 2 cut(s) 195, 230
MvaI CCWGG 1 cut(s) 230
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 9 cut(s) 13, 190, 214, 225, 362, 464, 686, 717, 762
NciI CCSGG 1 cut(s) 195
NcoI CCATGG 1 cut(s) 265
NdeII GATC 1 cut(s) 630
NlaIII CATG 4 cut(s) 24, 217, 269, 756
NlaIV GGNNCC 3 cut(s) 132, 262, 550
NspI RCATGY 2 cut(s) 24, 756
NspV TTCGAA 1 cut(s) 333
PaeR7I CTCGAG 1 cut(s) 26
PceI AGGCCT 1 cut(s) 401
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PfeI GAWTC 1 cut(s) 529
PleI GAGTC 2 cut(s) 560, 586
PpsI GAGTC 2 cut(s) 560, 586
Ppu21I YACGTR 1 cut(s) 94
Psp124BI GAGCTC 1 cut(s) 237
Psp6I CCWGG 1 cut(s) 228
PspFI CCCAGC 1 cut(s) 612
PspGI CCWGG 1 cut(s) 228
PspN4I GGNNCC 3 cut(s) 132, 262, 550
PspPI GGNCC 1 cut(s) 494
RsaI GTAC 2 cut(s) 104, 137
RsaNI GTAC 2 cut(s) 103, 136
SacI GAGCTC 1 cut(s) 237
SalI GTCGAC 2 cut(s) 696, 772
SaqAI TTAA 1 cut(s) 743
Sau3AI GATC 1 cut(s) 630
Sau96I GGNCC 1 cut(s) 494
SchI GAGTC 2 cut(s) 560, 587
ScrFI CCNGG 2 cut(s) 195, 230
SduI GDGCHC 3 cut(s) 133, 186, 237
SfaNI GCATC 5 cut(s) 579, 667, 698, 737, 774
Sfr274I CTCGAG 1 cut(s) 26
SfuI TTCGAA 1 cut(s) 333
SgrAI CRCCGGYG 1 cut(s) 551
SlaI CTCGAG 1 cut(s) 26
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
Sse9I AATT 4 cut(s) 243, 498, 658, 668
SseBI AGGCCT 1 cut(s) 401
SspMI CTAG 2 cut(s) 723, 799
SstI GAGCTC 1 cut(s) 237
StuI AGGCCT 1 cut(s) 401
StyD4I CCNGG 2 cut(s) 193, 228
StyI CCWWGG 2 cut(s) 265, 402
TaaI ACNGT 1 cut(s) 377
TaiI ACGT 1 cut(s) 96
TaqI TCGA 6 cut(s) 27, 333, 576, 633, 697, 773
TasI AATT 4 cut(s) 243, 498, 658, 668
TauI GCSGC 2 cut(s) 258, 602
TfiI GAWTC 1 cut(s) 529
Tru1I TTAA 1 cut(s) 743
Tru9I TTAA 1 cut(s) 743
TscAI CASTG 1 cut(s) 475
TspDTI ATGAA 2 cut(s) 304, 492
TspGWI ACGGA 4 cut(s) 123, 373, 532, 578
TspRI CASTG 1 cut(s) 475
XagI CCTNNNNNAGG 1 cut(s) 94
XapI RAATTY 1 cut(s) 243
XceI RCATGY 2 cut(s) 24, 756
XhoI CTCGAG 1 cut(s) 26
XmiI GTMKAC 2 cut(s) 697, 773
XspI CTAG 2 cut(s) 723, 799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.