Rh6BG313600

Cell division protein kinase. Source PGD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0018656
Physical Location & Seq
Reverse (-)
54356178 .. 54358573
2396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG313600.1

Sequence Viewer

Length: 840 bp
ATGGCTTTTCCATCAGCGTTGGAGTCCAGATTTCATGATGTGGAGCTCATCGGCCAAGGTCCTCGGTTCGTTGTGTATGGCGCCAAGCTTAAGAATTCCGCCATCAGAGTTGCTGTAAAGGTCATGAGGCCAGAGGTGCTTGCATGTCTCGAGGGTCGGAGTGCCATCTCTTCCGCTCTCCAGTCCGAGCTGCTGAAATCCTCGTCGGCTGCCTACGTGAGGCTGTACCTCTACGACTACGGCAGCGACGGGGCTCCGTACTTTTTGTATGAGCGGATGGACGAGAACCTCACGGGAAAAGGAGAGCCCCGAGAGCCGGGTTTGGTCAAGCAGCTCATGCGCGAGCTTTGCCAGGAGCTCGCCGGAATTCACTCCAGCGGCGGAACCCATGGTGCGCTCCACCCAAACAACTTGTTCTTGAAGGATGGACATCTGTTCATCGGAGATTACGGGTTCGAAGGAGCTTTCCCTGTTCCAGCTACCGCAGCAATGCCATACGGTTACTCCGTCATCCGCTACAAGGCCTTGGAGAGAAAGAGGACGCCAAGCGGGGATATTTGGGCAGCGGGCTGTGTTTTTGCGGAGCTGGCCACTGGAAGCCCTCTCTTCACCTCAGGGCCAATTTCATCGCAGAAGGAACGGATTTACCGGATTCTTGGAAGGAGGGCTGGCACCGGCGACATCACGGACTCTGACATCGAGTCCTGTAAGATGCGTTTGGCGGCTGCTGTTCCCCAGCTTTCACAGGAAGGGATCGACCTCCTTTATGAGATGCTGCAAGAACATCCCGTCGACCGGATTGGTGCGGAGGCAGCACTAGAGCATCCTTTTTTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

279

Amino Acids

30.52

Weight (kDa)

6.01

Isoelectric Point (pI)

39.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 11 - 278 9.8e-23 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018656)

Species Orthologous Gene IDs
rosa_laevigata RLG00000012355 RLG00000012357
rosa_roxburghii Rroxscaffold_7G00179800
rosa_rugosa Rorug06G0200200 Rorug06G0200200 Rorug06G0200300
rosa_samantha Rh6AG310300 Rh6BG313600 Rh6BG316200 Rh6BG316500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 80, 671
AccBSI CCGCTC 2 cut(s) 176, 274
AccI GTMKAC 1 cut(s) 792
AccII CGCG 1 cut(s) 342
AclWI GGATC 1 cut(s) 761
AcoI YGGCCR 2 cut(s) 52, 588
AcsI RAATTY 2 cut(s) 94, 366
AcyI GRCGYC 2 cut(s) 81, 542
AfaI GTAC 2 cut(s) 227, 260
AfiI CCNNNNNNNGG 4 cut(s) 219, 316, 520, 795
AflII CTTAAG 1 cut(s) 89
AgsI TTSAA 1 cut(s) 421
AhdI GACNNNNNGTC 1 cut(s) 700
AjnI CCWGG 1 cut(s) 351
Alw21I GWGCWC 2 cut(s) 48, 360
Alw26I GTCTC 1 cut(s) 152
AlwI GGATC 1 cut(s) 761
Ama87I CYCGRG 2 cut(s) 149, 309
AoxI GGCC 5 cut(s) 52, 128, 522, 588, 617
ApeKI GCWGC 9 cut(s) 190, 209, 243, 331, 485, 563, 725, 775, 812
ApoI RAATTY 2 cut(s) 94, 366
AspLEI GCGC 3 cut(s) 83, 342, 397
AspS9I GGNCC 2 cut(s) 59, 617
AsuC2I CCSGG 1 cut(s) 318
AsuHPI GGTGA 1 cut(s) 601
AsuII TTCGAA 1 cut(s) 456
AvaI CYCGRG 2 cut(s) 149, 309
AvaII GGWCC 1 cut(s) 59
AxyI CCTNAGG 1 cut(s) 613
BalI TGGCCA 1 cut(s) 590
BanI GGYRCC 2 cut(s) 80, 671
BanII GRGCYC 4 cut(s) 48, 256, 309, 360
Bbv12I GWGCWC 2 cut(s) 48, 360
BbvI GCAGC 9 cut(s) 177, 196, 255, 343, 497, 575, 712, 762, 824
BccI CCATC 5 cut(s) 19, 110, 173, 271, 419
BceAI ACGGC 1 cut(s) 256
BciT130I CCWGG 1 cut(s) 353
BcnI CCSGG 1 cut(s) 318
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 1 cut(s) 818
BfoI RGCGCY 1 cut(s) 84
BfrI CTTAAG 1 cut(s) 89
Bme1390I CCNGG 2 cut(s) 318, 353
Bme18I GGWCC 1 cut(s) 59
BmeRI GACNNNNNGTC 1 cut(s) 700
BmeT110I CYCGRG 2 cut(s) 149, 309
BmgT120I GGNCC 2 cut(s) 59, 617
BmiI GGNNCC 4 cut(s) 82, 255, 385, 673
BmrFI CCNGG 2 cut(s) 318, 353
BmsI GCATC 3 cut(s) 702, 762, 832
BpmI CTGGAG 2 cut(s) 164, 358
Bpu14I TTCGAA 1 cut(s) 456
BpuMI CCSGG 1 cut(s) 318
BsaAI YACGTR 1 cut(s) 217
BsaBI GATNNNNATC 1 cut(s) 429
BsaHI GRCGYC 2 cut(s) 81, 542
BsaJI CCNNGG 4 cut(s) 55, 62, 388, 525
BsaWI WCCGGW 2 cut(s) 648, 795
BsaXI ACNNNNNCTCC 2 cut(s) 488, 518
Bsc4I CCNNNNNNNGG 4 cut(s) 219, 316, 520, 795
Bse118I RCCGGY 1 cut(s) 674
Bse1I ACTGG 2 cut(s) 181, 598
Bse21I CCTNAGG 1 cut(s) 613
Bse3DI GCAATG 1 cut(s) 495
Bse8I GATNNNNATC 1 cut(s) 429
BseBI CCWGG 1 cut(s) 353
BseDI CCNNGG 4 cut(s) 55, 62, 388, 525
BseGI GGATG 5 cut(s) 282, 430, 510, 784, 823
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 4 cut(s) 219, 316, 520, 795
BseMI GCAATG 1 cut(s) 495
BseMII CTCAG 1 cut(s) 627
BseNI ACTGG 2 cut(s) 181, 598
BseXI GCAGC 9 cut(s) 177, 196, 255, 343, 497, 575, 712, 762, 824
BseYI CCCAGC 1 cut(s) 735
Bsh1236I CGCG 1 cut(s) 342
Bsh1285I CGRYCG 1 cut(s) 796
BshFI GGCC 5 cut(s) 54, 130, 524, 590, 619
BshNI GGYRCC 2 cut(s) 80, 671
BsiEI CGRYCG 1 cut(s) 796
BsiHKAI GWGCWC 2 cut(s) 48, 360
BsiHKCI CYCGRG 2 cut(s) 149, 309
BsiSI CCGG 5 cut(s) 317, 363, 649, 675, 796
BslI CCNNNNNNNGG 4 cut(s) 219, 316, 520, 795
BsmAI GTCTC 1 cut(s) 152
BsnI GGCC 5 cut(s) 54, 130, 524, 590, 619
BsoBI CYCGRG 2 cut(s) 149, 309
Bsp119I TTCGAA 1 cut(s) 456
Bsp1286I GDGCHC 4 cut(s) 48, 256, 309, 360
Bsp143I GATC 1 cut(s) 753
Bsp19I CCATGG 1 cut(s) 388
BspANI GGCC 5 cut(s) 54, 130, 524, 590, 619
BspCNI CTCAG 1 cut(s) 626
BspFNI CGCG 1 cut(s) 342
BspHI TCATGA 2 cut(s) 34, 123
BspLI GGNNCC 4 cut(s) 82, 255, 385, 673
BspPI GGATC 1 cut(s) 761
BspT104I TTCGAA 1 cut(s) 456
BspT107I GGYRCC 2 cut(s) 80, 671
BspTI CTTAAG 1 cut(s) 89
BsrBI CCGCTC 2 cut(s) 176, 274
BsrDI GCAATG 1 cut(s) 495
BsrFI RCCGGY 1 cut(s) 674
BsrI ACTGG 2 cut(s) 181, 598
BssAI RCCGGY 1 cut(s) 674
BssECI CCNNGG 4 cut(s) 55, 62, 388, 525
BssMI GATC 1 cut(s) 753
BssNI GRCGYC 2 cut(s) 81, 542
BssT1I CCWWGG 3 cut(s) 55, 388, 525
Bst2UI CCWGG 1 cut(s) 353
Bst4CI ACNGT 1 cut(s) 500
Bst6I CTCTTC 2 cut(s) 175, 611
BstACI GRCGYC 2 cut(s) 81, 542
BstAFI CTTAAG 1 cut(s) 89
BstAPI GCANNNNNTGC 1 cut(s) 337
BstBAI YACGTR 1 cut(s) 217
BstBI TTCGAA 1 cut(s) 456
BstC8I GCNNGC 6 cut(s) 141, 344, 360, 568, 588, 670
BstDEI CTNAG 1 cut(s) 613
BstDSI CCRYGG 1 cut(s) 388
BstENI CCTNNNNNAGG 1 cut(s) 217
BstF5I GGATG 5 cut(s) 282, 430, 510, 784, 823
BstFNI CGCG 1 cut(s) 342
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 3 cut(s) 83, 342, 397
BstKTI GATC 1 cut(s) 756
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 1 cut(s) 753
BstMCI CGRYCG 1 cut(s) 796
BstMWI GCNNNNNNNGC 7 cut(s) 136, 313, 337, 348, 485, 587, 812
BstNI CCWGG 1 cut(s) 353
BstNSI RCATGY 1 cut(s) 147
BstSCI CCNGG 2 cut(s) 316, 351
BstUI CGCG 1 cut(s) 342
BstV1I GCAGC 9 cut(s) 177, 196, 255, 343, 497, 575, 712, 762, 824
Bsu36I CCTNAGG 1 cut(s) 613
BsuRI GGCC 5 cut(s) 54, 130, 524, 590, 619
BtgI CCRYGG 1 cut(s) 388
BtgZI GCGATG 1 cut(s) 612
BtsCI GGATG 5 cut(s) 282, 430, 510, 784, 823
BtsIMutI CAGTG 1 cut(s) 591
Cac8I GCNNGC 6 cut(s) 141, 344, 360, 568, 588, 670
CciI TCATGA 2 cut(s) 34, 123
CfoI GCGC 3 cut(s) 83, 342, 397
Cfr10I RCCGGY 1 cut(s) 674
Cfr13I GGNCC 2 cut(s) 59, 617
CseI GACGC 1 cut(s) 550
Csp6I GTAC 2 cut(s) 226, 259
CviAII CATG 5 cut(s) 35, 124, 144, 337, 389
CviQI GTAC 2 cut(s) 226, 259
DdeI CTNAG 1 cut(s) 613
DinI GGCGCC 1 cut(s) 82
DpnI GATC 1 cut(s) 755
DpnII GATC 1 cut(s) 753
DriI GACNNNNNGTC 1 cut(s) 700
EaeI YGGCCR 2 cut(s) 52, 588
Eam1104I CTCTTC 2 cut(s) 175, 611
Eam1105I GACNNNNNGTC 1 cut(s) 700
EarI CTCTTC 2 cut(s) 175, 611
EciI GGCGGA 2 cut(s) 88, 396
Ecl136II GAGCTC 2 cut(s) 46, 358
Eco130I CCWWGG 3 cut(s) 55, 388, 525
Eco147I AGGCCT 1 cut(s) 524
Eco24I GRGCYC 4 cut(s) 48, 256, 309, 360
Eco47I GGWCC 1 cut(s) 59
Eco53kI GAGCTC 2 cut(s) 46, 358
Eco81I CCTNAGG 1 cut(s) 613
Eco88I CYCGRG 2 cut(s) 149, 309
EcoICRI GAGCTC 2 cut(s) 46, 358
EcoNI CCTNNNNNAGG 1 cut(s) 217
EcoO109I RGGNCCY 1 cut(s) 59
EcoRI GAATTC 2 cut(s) 94, 366
EcoRII CCWGG 1 cut(s) 351
EcoT14I CCWWGG 3 cut(s) 55, 388, 525
EcoT38I GRGCYC 4 cut(s) 48, 256, 309, 360
EgeI GGCGCC 1 cut(s) 82
EheI GGCGCC 1 cut(s) 82
ErhI CCWWGG 3 cut(s) 55, 388, 525
FaeI CATG 5 cut(s) 38, 127, 147, 340, 392
FaiI YATR 9 cut(s) 36, 78, 125, 145, 270, 338, 390, 496, 768
FatI CATG 5 cut(s) 34, 123, 143, 336, 388
FauI CCCGC 2 cut(s) 542, 559
FblI GTMKAC 1 cut(s) 792
FokI GGATG 5 cut(s) 289, 437, 497, 771, 810
FriOI GRGCYC 4 cut(s) 48, 256, 309, 360
FspBI CTAG 1 cut(s) 818
GlaI GCGC 3 cut(s) 82, 341, 396
GsaI CCCAGC 1 cut(s) 739
GsuI CTGGAG 2 cut(s) 164, 358
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 5 cut(s) 54, 130, 524, 590, 619
HapII CCGG 5 cut(s) 317, 363, 649, 675, 796
HgaI GACGC 1 cut(s) 550
HhaI GCGC 3 cut(s) 83, 342, 397
Hin1I GRCGYC 2 cut(s) 81, 542
Hin1II CATG 5 cut(s) 38, 127, 147, 340, 392
Hin6I GCGC 3 cut(s) 81, 340, 395
HinP1I GCGC 3 cut(s) 81, 340, 395
HincII GTYRAC 1 cut(s) 793
HindII GTYRAC 1 cut(s) 793
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 4 cut(s) 23, 652, 689, 701
HpaII CCGG 5 cut(s) 317, 363, 649, 675, 796
HphI GGTGA 1 cut(s) 601
Hpy166II GTNNAC 1 cut(s) 793
Hpy188I TCNGA 5 cut(s) 107, 159, 187, 443, 694
Hpy188III TCNNGA 5 cut(s) 27, 35, 124, 149, 418
Hpy8I GTNNAC 1 cut(s) 793
Hpy99I CGWCG 3 cut(s) 208, 251, 794
HpyAV CCTTC 5 cut(s) 415, 452, 628, 654, 743
HpyCH4III ACNGT 1 cut(s) 500
HpyCH4IV ACGT 1 cut(s) 216
HpyCH4V TGCA 2 cut(s) 143, 778
HpyF10VI GCNNNNNNNGC 7 cut(s) 136, 313, 337, 348, 485, 587, 812
HpyF3I CTNAG 1 cut(s) 613
HpySE526I ACGT 1 cut(s) 216
Hsp92I GRCGYC 2 cut(s) 81, 542
Hsp92II CATG 5 cut(s) 38, 127, 147, 340, 392
HspAI GCGC 3 cut(s) 81, 340, 395
KasI GGCGCC 1 cut(s) 80
Kzo9I GATC 1 cut(s) 753
LmnI GCTCC 6 cut(s) 43, 259, 355, 402, 461, 583
Lsp1109I GCAGC 9 cut(s) 177, 196, 255, 343, 497, 575, 712, 762, 824
LweI GCATC 3 cut(s) 702, 762, 832
MaeI CTAG 1 cut(s) 818
MaeII ACGT 1 cut(s) 216
MaeIII GTNAC 1 cut(s) 500
MalI GATC 1 cut(s) 755
MbiI CCGCTC 2 cut(s) 176, 274
MboI GATC 1 cut(s) 753
MboII GAAGA 2 cut(s) 162, 598
MhlI GDGCHC 4 cut(s) 48, 256, 309, 360
MlsI TGGCCA 1 cut(s) 590
MluCI AATT 3 cut(s) 94, 366, 621
MluNI TGGCCA 1 cut(s) 590
Mly113I GGCGCC 1 cut(s) 81
MlyI GAGTC 3 cut(s) 32, 683, 710
MmeI TCCRAC 1 cut(s) 137
Mox20I TGGCCA 1 cut(s) 590
MscI TGGCCA 1 cut(s) 590
MseI TTAA 1 cut(s) 90
Msp20I TGGCCA 1 cut(s) 590
MspA1I CMGCKG 2 cut(s) 378, 566
MspCI CTTAAG 1 cut(s) 89
MspI CCGG 5 cut(s) 317, 363, 649, 675, 796
MspR9I CCNGG 2 cut(s) 318, 353
MvaI CCWGG 1 cut(s) 353
MvnI CGCG 1 cut(s) 342
MwoI GCNNNNNNNGC 7 cut(s) 136, 313, 337, 348, 485, 587, 812
NarI GGCGCC 1 cut(s) 81
NciI CCSGG 1 cut(s) 318
NcoI CCATGG 1 cut(s) 388
NdeII GATC 1 cut(s) 753
NlaIII CATG 5 cut(s) 38, 127, 147, 340, 392
NlaIV GGNNCC 4 cut(s) 82, 255, 385, 673
NspI RCATGY 1 cut(s) 147
NspV TTCGAA 1 cut(s) 456
PaeR7I CTCGAG 1 cut(s) 149
PagI TCATGA 2 cut(s) 34, 123
PceI AGGCCT 1 cut(s) 524
PcsI WCGNNNNNNNCGW 1 cut(s) 504
PfeI GAWTC 1 cut(s) 652
PleI GAGTC 3 cut(s) 31, 683, 709
PluTI GGCGCC 1 cut(s) 84
PpsI GAGTC 3 cut(s) 31, 683, 709
Ppu21I YACGTR 1 cut(s) 217
PpuMI RGGWCCY 1 cut(s) 59
Psp124BI GAGCTC 2 cut(s) 48, 360
Psp5II RGGWCCY 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 351
PspFI CCCAGC 1 cut(s) 735
PspGI CCWGG 1 cut(s) 351
PspN4I GGNNCC 4 cut(s) 82, 255, 385, 673
PspPI GGNCC 2 cut(s) 59, 617
PspPPI RGGWCCY 1 cut(s) 59
RsaI GTAC 2 cut(s) 227, 260
RsaNI GTAC 2 cut(s) 226, 259
SacI GAGCTC 2 cut(s) 48, 360
SalI GTCGAC 1 cut(s) 791
SaqAI TTAA 1 cut(s) 90
Sau3AI GATC 1 cut(s) 753
Sau96I GGNCC 2 cut(s) 59, 617
SchI GAGTC 3 cut(s) 32, 683, 710
ScrFI CCNGG 2 cut(s) 318, 353
SduI GDGCHC 4 cut(s) 48, 256, 309, 360
SfaNI GCATC 3 cut(s) 702, 762, 832
SfoI GGCGCC 1 cut(s) 82
Sfr274I CTCGAG 1 cut(s) 149
SfuI TTCGAA 1 cut(s) 456
SgrAI CRCCGGYG 1 cut(s) 674
SinI GGWCC 1 cut(s) 59
SlaI CTCGAG 1 cut(s) 149
SmlI CTYRAG 2 cut(s) 89, 149
SmoI CTYRAG 2 cut(s) 89, 149
Sse9I AATT 3 cut(s) 94, 366, 621
SseBI AGGCCT 1 cut(s) 524
SspDI GGCGCC 1 cut(s) 80
SspMI CTAG 1 cut(s) 818
SstI GAGCTC 2 cut(s) 48, 360
StuI AGGCCT 1 cut(s) 524
StyD4I CCNGG 2 cut(s) 316, 351
StyI CCWWGG 3 cut(s) 55, 388, 525
TaaI ACNGT 1 cut(s) 500
TaiI ACGT 1 cut(s) 219
TaqI TCGA 5 cut(s) 150, 456, 699, 756, 792
TasI AATT 3 cut(s) 94, 366, 621
TauI GCSGC 2 cut(s) 381, 725
TfiI GAWTC 1 cut(s) 652
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TscAI CASTG 1 cut(s) 598
TseI GCWGC 9 cut(s) 190, 209, 243, 331, 485, 563, 725, 775, 812
TspDTI ATGAA 3 cut(s) 23, 427, 615
TspGWI ACGGA 4 cut(s) 246, 496, 655, 701
TspRI CASTG 1 cut(s) 598
Vha464I CTTAAG 1 cut(s) 89
VpaK11BI GGWCC 1 cut(s) 59
XagI CCTNNNNNAGG 1 cut(s) 217
XapI RAATTY 2 cut(s) 94, 366
XceI RCATGY 1 cut(s) 147
XhoI CTCGAG 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 792
XspI CTAG 1 cut(s) 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.