Rh6BG316500

Cell division protein kinase. Source PGD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0018656
Physical Location & Seq
Forward (+)
54682029 .. 54685711
3683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG316500.1

Sequence Viewer

Length: 792 bp
ATGGCTTTTCCATCAGCGTTGGAGTCCAGATTTCATGATGTGGAGCTCATCGGCCAAGGTCCTCGGTTCGTTGTCTATGGCGCGAAGCTGAAGAATTCCGCCATCAGAGTTGCTGTGAAGGTGATGAGGCCAGAGGTGCTTGCATGTCTCGAGACGGGTCGGAGTGCCATCTCTTCCGCTCTCCAGTCCGAGCTGCTGAAATCCTCGTCGGCTGCGTACGTGAGGCTTTACTTCTACGACTACGGCAGCGACGGCGCTCCATACTTTGTGTATGACCGGATGGACGAGAATCTCACGGGAAAAGGAGAGCCCCGAGAGCCGGCTTTGGTGAAGCAGGTGATGCGCGAGCTTTGCCAGGAGCTCGCCGGAATTCACTCCAGCGGCAGAACCCACGGTGCGCTCCACCCAAACAACTTGTTCCTGAAGGATGGACATCTGTTCATCGGAGATTACGGGTTCGACGGAGATTTCCCTGTTCCGGCTACGGCAGCAATGTCATATGCGTACTCCGTCATCCGCTACAAGGCTAGGGAGAAAAAGAAGACGGCAAGCGGCGATATTTGGGCGGCGGGCTGTGTTTTTGCCGAGCTGGCCACTGGAAACCCTCTCTTCACTTCAGGCCCAATTTCATCGCAGAAAGAACGGATTTACGAGTACACTCTCGGCTATTTCTGTTCTCACAAAGAATTTTCTACAGAATTTTTTTGGGAAAATTGTTTTATTTCTCACAGAAAATCAATTCTGCAGGATTCTTGGAAGGAGGGCCGGCACCGGCGACATCACGGACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

263

Amino Acids

29.53

Weight (kDa)

8.49

Isoelectric Point (pI)

37.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 11 - 219 5.4e-17 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018656)

Species Orthologous Gene IDs
rosa_laevigata RLG00000012355 RLG00000012357
rosa_roxburghii Rroxscaffold_7G00179800
rosa_rugosa Rorug06G0200200 Rorug06G0200200 Rorug06G0200300
rosa_samantha Rh6AG310300 Rh6BG313600 Rh6BG316200 Rh6BG316500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 325
Acc36I ACCTGC 1 cut(s) 325
AccB1I GGYRCC 1 cut(s) 768
AccBSI CCGCTC 1 cut(s) 179
AccII CGCG 2 cut(s) 83, 345
AciI CCGC 7 cut(s) 99, 177, 381, 517, 552, 566, 569
AcoI YGGCCR 2 cut(s) 52, 591
AcsI RAATTY 4 cut(s) 94, 369, 686, 698
AcuI CTGAAG 3 cut(s) 110, 443, 600
AfaI GTAC 3 cut(s) 218, 506, 656
AfiI CCNNNNNNNGG 3 cut(s) 319, 478, 523
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 6 cut(s) 46, 88, 193, 349, 361, 589
AluI AGCT 6 cut(s) 46, 88, 193, 349, 361, 589
Alw21I GWGCWC 2 cut(s) 48, 363
Alw26I GTCTC 2 cut(s) 146, 152
Ama87I CYCGRG 2 cut(s) 149, 312
AoxI GGCC 5 cut(s) 52, 128, 591, 619, 763
ApeKI GCWGC 4 cut(s) 193, 212, 246, 488
ApoI RAATTY 4 cut(s) 94, 369, 686, 698
AspLEI GCGC 4 cut(s) 83, 257, 345, 400
AspS9I GGNCC 3 cut(s) 59, 620, 763
AsuHPI GGTGA 3 cut(s) 133, 340, 349
AvaI CYCGRG 2 cut(s) 149, 312
AvaII GGWCC 1 cut(s) 59
BalI TGGCCA 1 cut(s) 593
BanI GGYRCC 1 cut(s) 768
BanII GRGCYC 3 cut(s) 48, 312, 363
BbsI GAAGAC 1 cut(s) 548
Bbv12I GWGCWC 2 cut(s) 48, 363
BbvI GCAGC 4 cut(s) 180, 199, 258, 500
BccI CCATC 5 cut(s) 19, 110, 176, 274, 422
BceAI ACGGC 4 cut(s) 259, 268, 501, 561
BciT130I CCWGG 1 cut(s) 356
BcoDI GTCTC 2 cut(s) 146, 152
BfaI CTAG 1 cut(s) 528
BfmI CTRYAG 2 cut(s) 693, 743
BfoI RGCGCY 1 cut(s) 258
BfuAI ACCTGC 1 cut(s) 325
BglI GCCNNNNNGGC 1 cut(s) 590
BisI GCNGC 7 cut(s) 194, 213, 247, 382, 489, 553, 567
BlsI GCNGC 7 cut(s) 195, 214, 248, 383, 490, 554, 568
Bme1390I CCNGG 1 cut(s) 356
Bme18I GGWCC 1 cut(s) 59
BmeT110I CYCGRG 2 cut(s) 149, 312
BmgT120I GGNCC 3 cut(s) 59, 620, 763
BmiI GGNNCC 1 cut(s) 770
BmrFI CCNGG 1 cut(s) 356
BmsI GCATC 1 cut(s) 330
BpiI GAAGAC 1 cut(s) 548
BpmI CTGGAG 2 cut(s) 167, 361
BsaAI YACGTR 1 cut(s) 220
BsaBI GATNNNNATC 1 cut(s) 432
BsaJI CCNNGG 3 cut(s) 55, 62, 391
BsaWI WCCGGW 1 cut(s) 276
Bsc4I CCNNNNNNNGG 3 cut(s) 319, 478, 523
Bse118I RCCGGY 3 cut(s) 319, 765, 771
Bse1I ACTGG 2 cut(s) 184, 601
Bse3DI GCAATG 1 cut(s) 498
Bse8I GATNNNNATC 1 cut(s) 432
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 3 cut(s) 55, 62, 391
BseGI GGATG 3 cut(s) 285, 433, 513
BseJI GATNNNNATC 1 cut(s) 432
BseLI CCNNNNNNNGG 3 cut(s) 319, 478, 523
BseMI GCAATG 1 cut(s) 498
BseNI ACTGG 2 cut(s) 184, 601
BseXI GCAGC 4 cut(s) 180, 199, 258, 500
Bsh1236I CGCG 2 cut(s) 83, 345
BshFI GGCC 5 cut(s) 54, 130, 593, 621, 765
BshNI GGYRCC 1 cut(s) 768
BsiHKAI GWGCWC 2 cut(s) 48, 363
BsiHKCI CYCGRG 2 cut(s) 149, 312
BsiSI CCGG 6 cut(s) 277, 320, 366, 479, 766, 772
BsiWI CGTACG 1 cut(s) 216
BslI CCNNNNNNNGG 3 cut(s) 319, 478, 523
BsmAI GTCTC 2 cut(s) 146, 152
BsmBI CGTCTC 1 cut(s) 146
BsnI GGCC 5 cut(s) 54, 130, 593, 621, 765
BsoBI CYCGRG 2 cut(s) 149, 312
Bsp1286I GDGCHC 3 cut(s) 48, 312, 363
BspACI CCGC 7 cut(s) 99, 177, 381, 517, 552, 566, 569
BspANI GGCC 5 cut(s) 54, 130, 593, 621, 765
BspFNI CGCG 2 cut(s) 83, 345
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 1 cut(s) 770
BspMAI CTGCAG 1 cut(s) 747
BspMI ACCTGC 1 cut(s) 325
BspT107I GGYRCC 1 cut(s) 768
BsrBI CCGCTC 1 cut(s) 179
BsrDI GCAATG 1 cut(s) 498
BsrFI RCCGGY 3 cut(s) 319, 765, 771
BsrI ACTGG 2 cut(s) 184, 601
BssAI RCCGGY 3 cut(s) 319, 765, 771
BssECI CCNNGG 3 cut(s) 55, 62, 391
BssT1I CCWWGG 1 cut(s) 55
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 1 cut(s) 395
Bst6I CTCTTC 2 cut(s) 178, 614
BstAPI GCANNNNNTGC 1 cut(s) 340
BstBAI YACGTR 1 cut(s) 220
BstC8I GCNNGC 8 cut(s) 141, 321, 347, 363, 550, 571, 591, 767
BstDSI CCRYGG 1 cut(s) 391
BstF5I GGATG 3 cut(s) 285, 433, 513
BstFNI CGCG 2 cut(s) 83, 345
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 4 cut(s) 83, 257, 345, 400
BstMAI GTCTC 2 cut(s) 146, 152
BstMWI GCNNNNNNNGC 7 cut(s) 136, 252, 316, 340, 351, 488, 590
BstNI CCWGG 1 cut(s) 356
BstNSI RCATGY 1 cut(s) 147
BstSCI CCNGG 1 cut(s) 354
BstSFI CTRYAG 2 cut(s) 693, 743
BstUI CGCG 2 cut(s) 83, 345
BstV1I GCAGC 4 cut(s) 180, 199, 258, 500
BstV2I GAAGAC 1 cut(s) 548
BsuRI GGCC 5 cut(s) 54, 130, 593, 621, 765
BtgI CCRYGG 1 cut(s) 391
BtgZI GCGATG 1 cut(s) 615
BtsCI GGATG 3 cut(s) 285, 433, 513
BtsIMutI CAGTG 1 cut(s) 594
BveI ACCTGC 1 cut(s) 325
Cac8I GCNNGC 8 cut(s) 141, 321, 347, 363, 550, 571, 591, 767
CciI TCATGA 1 cut(s) 34
CfoI GCGC 4 cut(s) 83, 257, 345, 400
Cfr10I RCCGGY 3 cut(s) 319, 765, 771
Cfr13I GGNCC 3 cut(s) 59, 620, 763
Csp6I GTAC 3 cut(s) 217, 505, 655
CviAII CATG 2 cut(s) 35, 144
CviQI GTAC 3 cut(s) 217, 505, 655
EaeI YGGCCR 2 cut(s) 52, 591
Eam1104I CTCTTC 2 cut(s) 178, 614
EarI CTCTTC 2 cut(s) 178, 614
EciI GGCGGA 1 cut(s) 88
Ecl136II GAGCTC 2 cut(s) 46, 361
Eco130I CCWWGG 1 cut(s) 55
Eco24I GRGCYC 3 cut(s) 48, 312, 363
Eco47I GGWCC 1 cut(s) 59
Eco53kI GAGCTC 2 cut(s) 46, 361
Eco57I CTGAAG 3 cut(s) 110, 443, 600
Eco88I CYCGRG 2 cut(s) 149, 312
EcoICRI GAGCTC 2 cut(s) 46, 361
EcoO109I RGGNCCY 1 cut(s) 59
EcoRI GAATTC 2 cut(s) 94, 369
EcoRII CCWGG 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 55
EcoT38I GRGCYC 3 cut(s) 48, 312, 363
ErhI CCWWGG 1 cut(s) 55
Esp3I CGTCTC 1 cut(s) 146
FaeI CATG 2 cut(s) 38, 147
FaiI YATR 7 cut(s) 36, 78, 145, 262, 273, 499, 501
FatI CATG 2 cut(s) 34, 143
FauI CCCGC 1 cut(s) 562
FauNDI CATATG 1 cut(s) 499
Fnu4HI GCNGC 7 cut(s) 194, 213, 247, 382, 489, 553, 567
FokI GGATG 3 cut(s) 292, 440, 500
FriOI GRGCYC 3 cut(s) 48, 312, 363
Fsp4HI GCNGC 7 cut(s) 194, 213, 247, 382, 489, 553, 567
FspBI CTAG 1 cut(s) 528
GlaI GCGC 4 cut(s) 82, 256, 344, 399
GluI GCNGC 7 cut(s) 194, 213, 247, 382, 489, 553, 567
GsuI CTGGAG 2 cut(s) 167, 361
HaeII RGCGCY 1 cut(s) 258
HaeIII GGCC 5 cut(s) 54, 130, 593, 621, 765
HapII CCGG 6 cut(s) 277, 320, 366, 479, 766, 772
HhaI GCGC 4 cut(s) 83, 257, 345, 400
Hin1II CATG 2 cut(s) 38, 147
Hin6I GCGC 4 cut(s) 81, 255, 343, 398
HinP1I GCGC 4 cut(s) 81, 255, 343, 398
HinfI GANTC 4 cut(s) 23, 289, 749, 786
HpaII CCGG 6 cut(s) 277, 320, 366, 479, 766, 772
HphI GGTGA 3 cut(s) 133, 340, 349
Hpy166II GTNNAC 1 cut(s) 657
Hpy188I TCNGA 5 cut(s) 107, 162, 190, 446, 791
Hpy188III TCNNGA 5 cut(s) 27, 35, 149, 151, 421
Hpy8I GTNNAC 1 cut(s) 657
Hpy99I CGWCG 3 cut(s) 211, 254, 464
HpyAV CCTTC 3 cut(s) 112, 418, 751
HpyCH4III ACNGT 1 cut(s) 395
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 2 cut(s) 143, 745
HpyF10VI GCNNNNNNNGC 7 cut(s) 136, 252, 316, 340, 351, 488, 590
HpySE526I ACGT 1 cut(s) 219
Hsp92II CATG 2 cut(s) 38, 147
HspAI GCGC 4 cut(s) 81, 255, 343, 398
KroI GCCGGC 2 cut(s) 319, 765
KroNI GCCGGC 2 cut(s) 321, 767
LmnI GCTCC 4 cut(s) 43, 262, 358, 405
Lsp1109I GCAGC 4 cut(s) 180, 199, 258, 500
LweI GCATC 1 cut(s) 330
MaeI CTAG 1 cut(s) 528
MaeII ACGT 1 cut(s) 219
MbiI CCGCTC 1 cut(s) 179
MboII GAAGA 4 cut(s) 103, 165, 553, 601
MhlI GDGCHC 3 cut(s) 48, 312, 363
MlsI TGGCCA 1 cut(s) 593
MluCI AATT 7 cut(s) 94, 369, 624, 686, 698, 712, 738
MluNI TGGCCA 1 cut(s) 593
MlyI GAGTC 2 cut(s) 32, 780
MmeI TCCRAC 1 cut(s) 140
MnlI CCTC 7 cut(s) 72, 120, 127, 214, 216, 615, 754
Mox20I TGGCCA 1 cut(s) 593
MroNI GCCGGC 2 cut(s) 319, 765
MscI TGGCCA 1 cut(s) 593
Msp20I TGGCCA 1 cut(s) 593
MspA1I CMGCKG 1 cut(s) 381
MspI CCGG 6 cut(s) 277, 320, 366, 479, 766, 772
MspR9I CCNGG 1 cut(s) 356
MvaI CCWGG 1 cut(s) 356
MvnI CGCG 2 cut(s) 83, 345
MwoI GCNNNNNNNGC 7 cut(s) 136, 252, 316, 340, 351, 488, 590
NaeI GCCGGC 2 cut(s) 321, 767
NdeI CATATG 1 cut(s) 499
NgoMIV GCCGGC 2 cut(s) 319, 765
NlaIII CATG 2 cut(s) 38, 147
NlaIV GGNNCC 1 cut(s) 770
NmeAIII GCCGAG 2 cut(s) 610, 642
NspI RCATGY 1 cut(s) 147
PaeR7I CTCGAG 1 cut(s) 149
PagI TCATGA 1 cut(s) 34
PaqCI CACCTGC 1 cut(s) 325
PcsI WCGNNNNNNNCGW 1 cut(s) 212
PdiI GCCGGC 2 cut(s) 321, 767
PfeI GAWTC 2 cut(s) 289, 749
Pfl23II CGTACG 1 cut(s) 216
PkrI GCNGC 7 cut(s) 195, 214, 248, 383, 490, 554, 568
PleI GAGTC 2 cut(s) 31, 780
PpsI GAGTC 2 cut(s) 31, 780
Ppu21I YACGTR 1 cut(s) 220
PpuMI RGGWCCY 1 cut(s) 59
Psp124BI GAGCTC 2 cut(s) 48, 363
Psp5II RGGWCCY 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 354
PspGI CCWGG 1 cut(s) 354
PspLI CGTACG 1 cut(s) 216
PspN4I GGNNCC 1 cut(s) 770
PspPI GGNCC 3 cut(s) 59, 620, 763
PspPPI RGGWCCY 1 cut(s) 59
PstI CTGCAG 1 cut(s) 747
RsaI GTAC 3 cut(s) 218, 506, 656
RsaNI GTAC 3 cut(s) 217, 505, 655
SacI GAGCTC 2 cut(s) 48, 363
SatI GCNGC 7 cut(s) 194, 213, 247, 382, 489, 553, 567
Sau96I GGNCC 3 cut(s) 59, 620, 763
SchI GAGTC 2 cut(s) 32, 780
ScrFI CCNGG 1 cut(s) 356
SduI GDGCHC 3 cut(s) 48, 312, 363
SfaNI GCATC 1 cut(s) 330
SfcI CTRYAG 2 cut(s) 693, 743
Sfr274I CTCGAG 1 cut(s) 149
SgrAI CRCCGGYG 1 cut(s) 771
SinI GGWCC 1 cut(s) 59
SlaI CTCGAG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 7 cut(s) 94, 369, 624, 686, 698, 712, 738
SsiI CCGC 7 cut(s) 99, 177, 381, 517, 552, 566, 569
SspMI CTAG 1 cut(s) 528
SstI GAGCTC 2 cut(s) 48, 363
StyD4I CCNGG 1 cut(s) 354
StyI CCWWGG 1 cut(s) 55
TaaI ACNGT 1 cut(s) 395
TaiI ACGT 1 cut(s) 222
TaqI TCGA 2 cut(s) 150, 459
TasI AATT 7 cut(s) 94, 369, 624, 686, 698, 712, 738
TatI WGTACW 1 cut(s) 654
TauI GCSGC 3 cut(s) 384, 555, 569
TfiI GAWTC 2 cut(s) 289, 749
TscAI CASTG 1 cut(s) 601
TseI GCWGC 4 cut(s) 193, 212, 246, 488
TspDTI ATGAA 3 cut(s) 23, 430, 618
TspGWI ACGGA 3 cut(s) 477, 499, 658
TspRI CASTG 1 cut(s) 601
VpaK11BI GGWCC 1 cut(s) 59
XapI RAATTY 4 cut(s) 94, 369, 686, 698
XceI RCATGY 1 cut(s) 147
XhoI CTCGAG 1 cut(s) 149
XspI CTAG 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.