RLG00000012757

Cell division control protein 45 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0016184
Physical Location & Seq
Forward (+)
22084244 .. 22086031
1788 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012757

Sequence Viewer

Length: 1698 bp
ATGGTTAGAGAGCAAACAGTGGAGTCCTTCTACTCGAGGCTCCGCGAATCGGCGCTAGCCTCGTCGGGTTCTCCTCTGCTCATATTCCCATCTACTTCAGATGTGGACTCGCTCTGTGCCGTCAAAATCATTTTACATGTGCTTGAATCGGATTCAGTCCGGTACTCGTGTTATCCGGTGTCTTCGTTTCAGGAGATTCACAAGTACGCAGGGTCGGACCTGTGTTCTTCATCGGAGAATCCGATTTGTATTCTTTTGATCAATTGGGGGTGTCACCGGGACCTGAGGAGGCTGCTGAATTTGTCTCCCTTGGCTCGTGTTTTCGTGGTGGACAGTCACCGGCCGATTCATTTGCATAATCTGAGTGAGCAGAATGATCGTGTGGTTGTGCTTTATACATTGGAAGATGAGAGGCAGGCGGATTTGGCCTATGATATTGAGGATGTTTCGGCTTTGGCTAATGCGAGTGACTTGAATAGTGATGATGAGCTTGATGGAGAATCGGATAGTGATGGTGATAGTGATAGTGAGAGTGAGGAGGATGAAGCTGGGGATGGGGATGGGTCGAGGAAGAAAAGGAGGGTATCTGAGGAAGAAGACCCGGTTAAGCTTTTTGGGAAGTTGAAGAAGAAATACTACCATCTGGGTACTTTTCATGGTAAGCCTTCGGGGTGTTTGATGTTGACAGATGAGAGGTACCAGGCTGCGGTTATGGAGCTTGAGCAGCATATTAACAGTTCAGGGAATTTGGAGGCAGTGACTTCGGTGACTCTCAAAGATGGGACGAAGATTCGGCTGCCCAGTTCGTCTAGAATTGCATATGAAGATGAACCTCGACTAATGCTGTTGCAGGAATGGAATTTATTCGATTCCATGATTTGTTCTTCATACATTGCAACCAAAATGAAGACATGGAGTGACAACGGAATGAAAAAGCTCCAGCTTCTTATTGCAAGGATGGGAATTGCGCTGGTGGATTGCCAACAGAAGTTTCAGTTCATGAATGTTGAAGTCAAAAAGAGGATGAGAGATCAATTTGAACAGTTTTTACCCGAGTATGGACTCACTGATTTCTACTACCGGAGCTTTCTGCGGCTCCACGGTTATGGCTCAAGGGTATCAGCAGCAGATGTGGTGTATGGAGTTACAGCTTTGCTTGAATCGTTTGTAGAGTCAGATGGTTCCTGTGCTGCTAAGCAGTTTGGGGTAGCTTATGATGCCCTGTCATTGAACAATCTTGATAAGCTGAAAGCTGGAATGCAGAAAGCAATTCGGGTACAAAGGGCAATTGTTAGACAAGGTAGTAGAGCAGTAACAAAGAGTGGTTGTATAAGAAGTGGAAGGAAATTTAGGTGGGTGAAACTTGAAGATTCTGTAGATGCGAAACTGTTGGGGTACCCCCAGGCATTGACTAAATTGTGCTATTTTGTGATGGATGCTCTGAAAGAGAAGGGAGCTAGAATGAAGCCTTTGGTTTGTGCTTGCTTGGCACAAGAAGCCAAAAAGATGATCATAGTTGGGGTTTGTGGGAAGCCTCGACTGGGAGCAGTTAAAGGAAATTCATTTGGAATTGCATTTCGAACCGCAGCTCAGGAGATTTCTGCAGAGTTCTTCCATGAGATGTTTGAGTCTTCATGGATCATCCTGGATTCAGCGCAAGTGAATACCTTTATGATGCGGTTGACTGATAAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000228 GO:0000278 GO:0000724 GO:0000725 GO:0000727 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003688 GO:0003690 GO:0003697 GO:0003824 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005656 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006267 GO:0006270 GO:0006275 GO:0006281 GO:0006302 GO:0006310 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0008156 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010948 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0019219 GO:0019222 GO:0022402 GO:0022607 GO:0023052 GO:0031261 GO:0031298 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031570 GO:0031573 GO:0031935 GO:0031938 GO:0031974 GO:0031981 GO:0032392 GO:0032508 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0033260 GO:0033262 GO:0033554 GO:0034622 GO:0034641 GO:0034645 GO:0035556 GO:0036387 GO:0036388 GO:0042770 GO:0043138 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0043596 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044773 GO:0044774 GO:0044786 GO:0045786 GO:0045787 GO:0045930 GO:0045931 GO:0045934 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051128 GO:0051171 GO:0051172 GO:0051252 GO:0051276 GO:0051716 GO:0051726 GO:0060255 GO:0060968 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0071103 GO:0071163 GO:0071704 GO:0071824 GO:0071840 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072428 GO:0080090 GO:0090068 GO:0090304 GO:0090329 GO:0097159 GO:0140097 GO:1900087 GO:1901360 GO:1901363 GO:1901576 GO:1901987 GO:1901989 GO:1901990 GO:1901992 GO:1902275 GO:1902292 GO:1902299 GO:1902315 GO:1902402 GO:1902403 GO:1902576 GO:1902806 GO:1902808 GO:1902969 GO:1902975 GO:1902977 GO:1903047 GO:1903463 GO:1903464 GO:1903506 GO:1990837 GO:2000045 GO:2000104 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 map04110 map04111 map04113
Pfam Domains
Protein Families
CDC45

Protein Analysis

566

Amino Acids

63.43

Weight (kDa)

5.98

Isoelectric Point (pI)

44.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC45 PF02724 27 - 200 1.3e-35 CDC45
CDC45 PF02724 229 - 564 2.2e-104 CDC45
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25100
fragaria_vesca FvH4_2g18640
malus_domestica MD05G1172300.v1.1 MD10G1160500.v1.1
prunus_persica Prupe.8G199700_v2.0.a1 Prupe.8G199700_v2.0.a1
pyrus_communis pycom05g15890 pycom10g13870
rosa_chinensis RchiOBHm_Chr6g0284201
rosa_laevigata RLG00000012757
rosa_multiflora Rmu_sc0003778.1_g000001
rosa_roxburghii Rroxscaffold_7G00184790
rosa_rugosa Rorug06G0161800
rosa_samantha Rh6BG275200
rosa_wichuraiana Rw6G023630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 696, 1395
AccB1I GGYRCC 2 cut(s) 696, 1395
AccII CGCG 1 cut(s) 45
AciI CCGC 6 cut(s) 43, 419, 707, 1093, 1584, 1678
AclWI GGATC 1 cut(s) 1646
AcoI YGGCCR 1 cut(s) 341
AcsI RAATTY 5 cut(s) 298, 745, 859, 1346, 1558
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 6 cut(s) 164, 206, 649, 698, 1278, 1397
AfiI CCNNNNNNNGG 4 cut(s) 49, 706, 1058, 1541
AflIII ACRYGT 1 cut(s) 136
AgsI TTSAA 8 cut(s) 146, 475, 625, 1010, 1040, 1160, 1231, 1367
AjnI CCWGG 3 cut(s) 699, 1401, 1644
AjuI GAANNNNNNNTTGG 2 cut(s) 975, 1007
Alw26I GTCTC 1 cut(s) 309
AlwI GGATC 1 cut(s) 1646
Ama87I CYCGRG 2 cut(s) 34, 1052
AoxI GGCC 2 cut(s) 341, 426
ApeKI GCWGC 7 cut(s) 292, 704, 724, 796, 1124, 1190, 1586
ApoI RAATTY 5 cut(s) 298, 745, 859, 1346, 1558
Asp700I GAANNNNTTC 1 cut(s) 863
Asp718I GGTACC 2 cut(s) 696, 1395
AspLEI GCGC 3 cut(s) 55, 970, 1657
AspS9I GGNCC 2 cut(s) 217, 280
AsuC2I CCSGG 2 cut(s) 278, 602
AsuHPI GGTGA 5 cut(s) 266, 329, 527, 778, 1369
AsuII TTCGAA 1 cut(s) 1579
AsuNHI GCTAGC 1 cut(s) 55
AvaI CYCGRG 2 cut(s) 34, 1052
AvaII GGWCC 2 cut(s) 217, 280
AxyI CCTNAGG 1 cut(s) 284
BaeI ACNNNNGTAYC 2 cut(s) 154, 187
BanI GGYRCC 2 cut(s) 696, 1395
BarI GAAGNNNNNNTAC 4 cut(s) 617, 620, 649, 652
BauI CACGAG 2 cut(s) 166, 315
BbsI GAAGAC 4 cut(s) 174, 603, 914, 1623
BbvI GCAGC 7 cut(s) 279, 691, 736, 783, 1136, 1177, 1598
BceAI ACGGC 1 cut(s) 104
BcgI CGANNNNNNTGC 6 cut(s) 334, 359, 368, 393, 744, 778
BciT130I CCWGG 3 cut(s) 701, 1403, 1646
BclI TGATCA 2 cut(s) 258, 1509
BcnI CCSGG 2 cut(s) 278, 602
BcoDI GTCTC 1 cut(s) 309
BfaI CTAG 3 cut(s) 56, 810, 1458
BfmI CTRYAG 2 cut(s) 1374, 1602
BfoI RGCGCY 1 cut(s) 56
BisI GCNGC 8 cut(s) 293, 705, 725, 797, 1094, 1125, 1191, 1587
BlpI GCTNAGC 1 cut(s) 1194
BlsI GCNGC 8 cut(s) 294, 706, 726, 798, 1095, 1126, 1192, 1588
Bme1390I CCNGG 5 cut(s) 278, 602, 701, 1403, 1646
Bme18I GGWCC 2 cut(s) 217, 280
BmeT110I CYCGRG 2 cut(s) 34, 1052
BmgT120I GGNCC 2 cut(s) 217, 280
BmiI GGNNCC 6 cut(s) 41, 281, 698, 1097, 1183, 1397
BmrFI CCNGG 5 cut(s) 278, 602, 701, 1403, 1646
BmrI ACTGGG 2 cut(s) 795, 1550
BmsI GCATC 4 cut(s) 1207, 1369, 1426, 1665
BmtI GCTAGC 1 cut(s) 59
BmuI ACTGGG 2 cut(s) 795, 1550
BpiI GAAGAC 4 cut(s) 174, 603, 914, 1623
BpmI CTGGAG 1 cut(s) 923
Bpu10I CCTNAGC 1 cut(s) 1590
Bpu1102I GCTNAGC 1 cut(s) 1194
Bpu14I TTCGAA 1 cut(s) 1579
BpuEI CTTGAG 2 cut(s) 740, 1096
BpuMI CCSGG 2 cut(s) 278, 602
BsaJI CCNNGG 3 cut(s) 309, 1099, 1401
BsaWI WCCGGW 3 cut(s) 159, 175, 1080
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 706, 1058, 1541
Bse118I RCCGGY 1 cut(s) 339
Bse1I ACTGG 2 cut(s) 801, 1545
Bse21I CCTNAGG 1 cut(s) 284
Bse3DI GCAATG 1 cut(s) 891
BseBI CCWGG 3 cut(s) 701, 1403, 1646
BseDI CCNNGG 3 cut(s) 309, 1099, 1401
BseGI GGATG 8 cut(s) 448, 547, 559, 565, 963, 1029, 1441, 1641
BseLI CCNNNNNNNGG 4 cut(s) 49, 706, 1058, 1541
BseMI GCAATG 1 cut(s) 891
BseMII CTCAG 4 cut(s) 275, 353, 579, 1604
BseNI ACTGG 2 cut(s) 801, 1545
BseRI GAGGAG 3 cut(s) 63, 301, 551
BseX3I CGGCCG 1 cut(s) 341
BseXI GCAGC 7 cut(s) 279, 691, 736, 783, 1136, 1177, 1598
BseYI CCCAGC 1 cut(s) 548
Bsh1236I CGCG 1 cut(s) 45
Bsh1285I CGRYCG 1 cut(s) 344
BshFI GGCC 2 cut(s) 343, 428
BshNI GGYRCC 2 cut(s) 696, 1395
BsiEI CGRYCG 1 cut(s) 344
BsiHKCI CYCGRG 2 cut(s) 34, 1052
BsiSI CCGG 6 cut(s) 160, 176, 277, 340, 602, 1081
BslFI GGGAC 2 cut(s) 293, 796
BslI CCNNNNNNNGG 4 cut(s) 49, 706, 1058, 1541
BsmAI GTCTC 1 cut(s) 309
BsmFI GGGAC 2 cut(s) 293, 796
BsmI GAATGC 1 cut(s) 1263
BsnI GGCC 2 cut(s) 343, 428
BsoBI CYCGRG 2 cut(s) 34, 1052
Bsp119I TTCGAA 1 cut(s) 1579
Bsp143I GATC 5 cut(s) 258, 376, 1030, 1509, 1638
Bsp1720I GCTNAGC 1 cut(s) 1194
BspACI CCGC 6 cut(s) 43, 419, 707, 1093, 1584, 1678
BspANI GGCC 2 cut(s) 343, 428
BspCNI CTCAG 4 cut(s) 276, 354, 580, 1603
BspFNI CGCG 1 cut(s) 45
BspHI TCATGA 1 cut(s) 999
BspLI GGNNCC 6 cut(s) 41, 281, 698, 1097, 1183, 1397
BspMAI CTGCAG 1 cut(s) 1606
BspOI GCTAGC 1 cut(s) 59
BspPI GGATC 1 cut(s) 1646
BspT104I TTCGAA 1 cut(s) 1579
BspT107I GGYRCC 2 cut(s) 696, 1395
BsrDI GCAATG 1 cut(s) 891
BsrFI RCCGGY 1 cut(s) 339
BsrI ACTGG 2 cut(s) 801, 1545
BssAI RCCGGY 1 cut(s) 339
BssECI CCNNGG 3 cut(s) 309, 1099, 1401
BssMI GATC 5 cut(s) 258, 376, 1030, 1509, 1638
BssSI CACGAG 2 cut(s) 166, 315
BssT1I CCWWGG 1 cut(s) 309
Bst2BI CACGAG 2 cut(s) 166, 315
Bst2UI CCWGG 3 cut(s) 701, 1403, 1646
Bst4CI ACNGT 6 cut(s) 19, 335, 737, 1044, 1103, 1389
BstBI TTCGAA 1 cut(s) 1579
BstC8I GCNNGC 3 cut(s) 57, 417, 1483
BstDEI CTNAG 5 cut(s) 284, 362, 588, 1194, 1590
BstDSI CCRYGG 1 cut(s) 1099
BstF5I GGATG 8 cut(s) 448, 547, 559, 565, 963, 1029, 1441, 1641
BstFNI CGCG 1 cut(s) 45
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 3 cut(s) 55, 970, 1657
BstKTI GATC 5 cut(s) 261, 379, 1033, 1512, 1641
BstMAI GTCTC 1 cut(s) 309
BstMBI GATC 5 cut(s) 258, 376, 1030, 1509, 1638
BstMCI CGRYCG 1 cut(s) 344
BstMWI GCNNNNNNNGC 5 cut(s) 425, 724, 1217, 1487, 1496
BstNI CCWGG 3 cut(s) 701, 1403, 1646
BstNSI RCATGY 1 cut(s) 140
BstSCI CCNGG 5 cut(s) 276, 600, 699, 1401, 1644
BstSFI CTRYAG 2 cut(s) 1374, 1602
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 7 cut(s) 279, 691, 736, 783, 1136, 1177, 1598
BstV2I GAAGAC 4 cut(s) 174, 603, 914, 1623
BstXI CCANNNNNNTGG 1 cut(s) 1106
BstZI CGGCCG 1 cut(s) 341
Bsu36I CCTNAGG 1 cut(s) 284
BsuRI GGCC 2 cut(s) 343, 428
BtgI CCRYGG 1 cut(s) 1099
BtsCI GGATG 8 cut(s) 448, 547, 559, 565, 963, 1029, 1441, 1641
BtsI GCAGTG 1 cut(s) 762
BtsIMutI CAGTG 3 cut(s) 24, 762, 1065
Cac8I GCNNGC 3 cut(s) 57, 417, 1483
CciI TCATGA 1 cut(s) 999
CfoI GCGC 3 cut(s) 55, 970, 1657
Cfr10I RCCGGY 1 cut(s) 339
Cfr13I GGNCC 2 cut(s) 217, 280
Csp6I GTAC 6 cut(s) 163, 205, 648, 697, 1277, 1396
CviAII CATG 7 cut(s) 137, 656, 874, 912, 1000, 1616, 1635
CviQI GTAC 6 cut(s) 163, 205, 648, 697, 1277, 1396
DdeI CTNAG 5 cut(s) 284, 362, 588, 1194, 1590
DpnI GATC 5 cut(s) 260, 378, 1032, 1511, 1640
DpnII GATC 5 cut(s) 258, 376, 1030, 1509, 1638
EaeI YGGCCR 1 cut(s) 341
EagI CGGCCG 1 cut(s) 341
EciI GGCGGA 1 cut(s) 434
EclXI CGGCCG 1 cut(s) 341
Eco130I CCWWGG 1 cut(s) 309
Eco47I GGWCC 2 cut(s) 217, 280
Eco52I CGGCCG 1 cut(s) 341
Eco57I CTGAAG 1 cut(s) 81
Eco81I CCTNAGG 1 cut(s) 284
Eco88I CYCGRG 2 cut(s) 34, 1052
EcoO109I RGGNCCY 1 cut(s) 280
EcoRII CCWGG 3 cut(s) 699, 1401, 1644
EcoT14I CCWWGG 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 309
FaeI CATG 7 cut(s) 140, 659, 877, 915, 1003, 1619, 1638
FaqI GGGAC 2 cut(s) 293, 796
FatI CATG 7 cut(s) 136, 655, 873, 911, 999, 1615, 1634
FauNDI CATATG 1 cut(s) 820
FbaI TGATCA 2 cut(s) 258, 1509
Fnu4HI GCNGC 8 cut(s) 293, 705, 725, 797, 1094, 1125, 1191, 1587
FokI GGATG 8 cut(s) 455, 554, 566, 572, 970, 1036, 1448, 1628
Fsp4HI GCNGC 8 cut(s) 293, 705, 725, 797, 1094, 1125, 1191, 1587
FspBI CTAG 3 cut(s) 56, 810, 1458
GlaI GCGC 3 cut(s) 54, 969, 1656
GluI GCNGC 8 cut(s) 293, 705, 725, 797, 1094, 1125, 1191, 1587
GsaI CCCAGC 1 cut(s) 552
GsuI CTGGAG 1 cut(s) 923
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 2 cut(s) 343, 428
HapII CCGG 6 cut(s) 160, 176, 277, 340, 602, 1081
HhaI GCGC 3 cut(s) 55, 970, 1657
Hin1II CATG 7 cut(s) 140, 659, 877, 915, 1003, 1619, 1638
Hin6I GCGC 3 cut(s) 53, 968, 1655
HinP1I GCGC 3 cut(s) 53, 968, 1655
HincII GTYRAC 2 cut(s) 684, 1683
HindII GTYRAC 2 cut(s) 684, 1683
HindIII AAGCTT 1 cut(s) 608
HpaII CCGG 6 cut(s) 160, 176, 277, 340, 602, 1081
HphI GGTGA 5 cut(s) 266, 329, 527, 778, 1369
Hpy166II GTNNAC 4 cut(s) 106, 331, 684, 1683
Hpy188III TCNNGA 5 cut(s) 191, 810, 1000, 1238, 1592
Hpy8I GTNNAC 4 cut(s) 106, 331, 684, 1683
Hpy99I CGWCG 1 cut(s) 67
HpyAV CCTTC 4 cut(s) 37, 675, 1335, 1444
HpyCH4III ACNGT 6 cut(s) 19, 335, 737, 1044, 1103, 1389
HpyCH4V TGCA 8 cut(s) 355, 818, 850, 896, 953, 1261, 1574, 1604
HpyF10VI GCNNNNNNNGC 5 cut(s) 425, 724, 1217, 1487, 1496
HpyF3I CTNAG 5 cut(s) 284, 362, 588, 1194, 1590
Hsp92II CATG 7 cut(s) 140, 659, 877, 915, 1003, 1619, 1638
HspAI GCGC 3 cut(s) 53, 968, 1655
KpnI GGTACC 2 cut(s) 700, 1399
Ksp22I TGATCA 2 cut(s) 258, 1509
Kzo9I GATC 5 cut(s) 258, 376, 1030, 1509, 1638
LmnI GCTCC 7 cut(s) 45, 715, 942, 1083, 1101, 1454, 1544
Lsp1109I GCAGC 7 cut(s) 279, 691, 736, 783, 1136, 1177, 1598
LweI GCATC 4 cut(s) 1207, 1369, 1426, 1665
MaeI CTAG 3 cut(s) 56, 810, 1458
MaeIII GTNAC 8 cut(s) 272, 335, 467, 757, 766, 917, 1144, 1312
MalI GATC 5 cut(s) 260, 378, 1032, 1511, 1640
MboI GATC 5 cut(s) 258, 376, 1030, 1509, 1638
MfeI CAATTG 2 cut(s) 262, 1287
MlyI GAGTC 6 cut(s) 32, 101, 763, 1056, 1181, 1637
MmeI TCCRAC 1 cut(s) 195
MroXI GAANNNNTTC 1 cut(s) 863
MseI TTAA 3 cut(s) 606, 732, 1551
MslI CAYNNNNRTG 1 cut(s) 1104
MspI CCGG 6 cut(s) 160, 176, 277, 340, 602, 1081
MspR9I CCNGG 5 cut(s) 278, 602, 701, 1403, 1646
MunI CAATTG 2 cut(s) 262, 1287
Mva1269I GAATGC 1 cut(s) 1263
MvaI CCWGG 3 cut(s) 701, 1403, 1646
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 5 cut(s) 425, 724, 1217, 1487, 1496
NciI CCSGG 2 cut(s) 278, 602
NdeI CATATG 1 cut(s) 820
NdeII GATC 5 cut(s) 258, 376, 1030, 1509, 1638
NheI GCTAGC 1 cut(s) 55
NlaIII CATG 7 cut(s) 140, 659, 877, 915, 1003, 1619, 1638
NlaIV GGNNCC 6 cut(s) 41, 281, 698, 1097, 1183, 1397
NmuCI GTSAC 6 cut(s) 272, 335, 467, 757, 766, 917
NspI RCATGY 1 cut(s) 140
NspV TTCGAA 1 cut(s) 1579
PaeR7I CTCGAG 1 cut(s) 34
PagI TCATGA 1 cut(s) 999
PciI ACATGT 1 cut(s) 136
PcsI WCGNNNNNNNCGW 1 cut(s) 239
PctI GAATGC 1 cut(s) 1263
PdmI GAANNNNTTC 1 cut(s) 863
PfoI TCCNGGA 1 cut(s) 1644
PkrI GCNGC 8 cut(s) 294, 706, 726, 798, 1095, 1126, 1192, 1588
PleI GAGTC 6 cut(s) 31, 101, 763, 1056, 1180, 1636
PpsI GAGTC 6 cut(s) 31, 101, 763, 1056, 1180, 1636
PpuMI RGGWCCY 1 cut(s) 280
PscI ACATGT 1 cut(s) 136
Psp5II RGGWCCY 1 cut(s) 280
Psp6I CCWGG 3 cut(s) 699, 1401, 1644
PspFI CCCAGC 1 cut(s) 548
PspGI CCWGG 3 cut(s) 699, 1401, 1644
PspN4I GGNNCC 6 cut(s) 41, 281, 698, 1097, 1183, 1397
PspPI GGNCC 2 cut(s) 217, 280
PspPPI RGGWCCY 1 cut(s) 280
PspXI VCTCGAGB 1 cut(s) 34
PsrI GAACNNNNNNTAC 2 cut(s) 1032, 1064
PstI CTGCAG 1 cut(s) 1606
RsaI GTAC 6 cut(s) 164, 206, 649, 698, 1278, 1397
RsaNI GTAC 6 cut(s) 163, 205, 648, 697, 1277, 1396
RseI CAYNNNNRTG 1 cut(s) 1104
SaqAI TTAA 3 cut(s) 606, 732, 1551
SatI GCNGC 8 cut(s) 293, 705, 725, 797, 1094, 1125, 1191, 1587
Sau3AI GATC 5 cut(s) 258, 376, 1030, 1509, 1638
Sau96I GGNCC 2 cut(s) 217, 280
SchI GAGTC 6 cut(s) 32, 101, 763, 1056, 1181, 1637
ScrFI CCNGG 5 cut(s) 278, 602, 701, 1403, 1646
SfaNI GCATC 4 cut(s) 1207, 1369, 1426, 1665
SfcI CTRYAG 2 cut(s) 1374, 1602
Sfr274I CTCGAG 1 cut(s) 34
SfuI TTCGAA 1 cut(s) 1579
SinI GGWCC 2 cut(s) 217, 280
SlaI CTCGAG 1 cut(s) 34
SmiMI CAYNNNNRTG 1 cut(s) 1104
SmlI CTYRAG 3 cut(s) 34, 719, 1111
SmoI CTYRAG 3 cut(s) 34, 719, 1111
SsiI CCGC 6 cut(s) 43, 419, 707, 1093, 1584, 1678
SspMI CTAG 3 cut(s) 56, 810, 1458
StyD4I CCNGG 5 cut(s) 276, 600, 699, 1401, 1644
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 6 cut(s) 19, 335, 737, 1044, 1103, 1389
TaqI TCGA 6 cut(s) 35, 566, 835, 867, 1537, 1579
TauI GCSGC 1 cut(s) 1096
Tru1I TTAA 3 cut(s) 606, 732, 1551
Tru9I TTAA 3 cut(s) 606, 732, 1551
TscAI CASTG 3 cut(s) 24, 762, 1072
TseFI GTSAC 6 cut(s) 272, 335, 467, 757, 766, 917
TseI GCWGC 7 cut(s) 292, 704, 724, 796, 1124, 1190, 1586
Tsp45I GTSAC 6 cut(s) 272, 335, 467, 757, 766, 917
TspGWI ACGGA 1 cut(s) 939
TspRI CASTG 3 cut(s) 24, 762, 1072
VpaK11BI GGWCC 2 cut(s) 217, 280
XapI RAATTY 5 cut(s) 298, 745, 859, 1346, 1558
XbaI TCTAGA 1 cut(s) 809
XceI RCATGY 1 cut(s) 140
XhoI CTCGAG 1 cut(s) 34
XmnI GAANNNNTTC 1 cut(s) 863
XspI CTAG 3 cut(s) 56, 810, 1458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.