Rw6G023630

Cell division control protein 45 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0016184
Physical Location & Seq
Reverse (-)
46253380 .. 46255576
2197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G023630.1

Sequence Viewer

Length: 1788 bp
ATGGTTAGAGAGCAAACAGTGGAGTCCTTCTACTCGAGGCTCCGCGAATCGGCGATAGCCTCGTCGGGTTCTCCTCTGCTCATATTCCCATCTACTTCAGATGTGGACTCTCTCTGTGCCCTCAAAATCATTTTACATGTGCTTGAATCGGATTCGGTCCGGTACTCGTGTTATCCGGTGTCTTCGTTTCAGGAGATTCACAAGTACGCAGGGCCGGACCTGTGTTCGTCGTCGGAGAATCCGATTTGTATTCTTTTGATCAATTGGGGGTGTCACCGGGACCTGAGGAGGCTGCTCAATTTGTCCCCCGTGGCGCGTGTTTTCGTGGTGGACAGTCATCGGCCGATTCATTTGCATAATCTGAGTGAGCAGAATGATCGTGTGGTTGTGCTTTATACATTGGAAGACGAGAGGCAGGCGGATTTGGCCTATGATATTGAGGATGTTTCGGCTCTGGCTAATGCGAGTGACTTGAATAGTGATGATGAGCTTGATGGGGAGTCGGATAGTGATGGCGATAGTGATAGTGAGAGTGAGGAGGATGAAGCTGGGGATGGGGATGGGTCGAGGAAGAAAAGGAGGGTGTCTGAGGAAGAAGACCCGGTTAAGCTTTTTGGGAAGTTGAAGAAGAAATACTACCATTTGGGTACTTTTCATGGTAAGCCTTCGGGGTGTTTGATGTATGATCTGTCACATTCTTTGAGGAAGAACACGAATGAGTTGCTTTGGTTGTCTTGTGTTTCTTTAACGGATCAGTTTGTACACGAGAGGTTAACAGATGAGAGGTACCAGGCTGCGGTTATGGAGCTTGAGCAGCATATTAACAGTTCAGGGAATTTGGAGGCAGTGACTTCGGTGACTCTTAAAGATGGGACGAAGATTCGGCTGCCCAGTTCGTCTAGAATTGCATATGAAGATGAACCTCGACTAATGCTGTTGCAGGAATGGAATTTATTCGATTCCATGATTTGTTCTTCATACATTGCAACCAAAATGAAGACATGGAGTGACAACGGAATGAAAAAGCTCCAGCTTCTTATTGCAAGGATGGGAATTGCGCTGGTGGATTGCCAACAGAAGTTTCAGTTCATGAATGTTGAAGTGAAAAAAAGGATGAGAGATCAATTTGAACAGTTTTTACCCGAGTATGGACTCACTGATTTCTACTACCGGAGCTTTCTGCGGCTCCACGGTTATGGTTCAAGGGTATCAGCAGCAGATGTGGTGTATGGAGTTACAGCTTTGCTTGAATCGTTTGTAGAGTCAGATGGTTCCTGTGCTGCTAAGCAGTTCGGGGTAGCTTATGACGCACTGTCATTGAACAATCTTGATAAGCTGAAAGCTGGAATGCAGAAAGCAATTCGGGTACAAAGGGCAATTGTTAGACAAGGAAGTAGAGCAGTAACAAAGAGTGGTTGTATAAGAAGTGGAAGGAAATTTAGGTGGGTGAAACTTGAAGATTCTGTAGATGCGAAACTGTTGGGGTACCCCCAGGCATTGACTAAATTGTGCTATTTTGTGATGGATGCTCTGAAAGAGAAGGGAGCTAGAATGAAGCCTTTGGTTTGTGCTTGCTTGGCACAAGAAGCCAAAAAGATGATCATAGTTGGGGTTTGTGGGAAGCCTCGACTGGGAGCAGTTAAAGGAAATTCCTTTGGAATTGCATTTCGAACTGCAGCTCAGGAGATTTCTGCAGAGTTCTTCCATGAGATGTTTGAGTCTTCATGGATCATCCTGGATTCAGCTCAAGTGAATACCTTTATGATGAGGTTGACTGATAGATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000228 GO:0000278 GO:0000724 GO:0000725 GO:0000727 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003688 GO:0003690 GO:0003697 GO:0003824 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005656 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006267 GO:0006270 GO:0006275 GO:0006281 GO:0006302 GO:0006310 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0008156 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010948 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0019219 GO:0019222 GO:0022402 GO:0022607 GO:0023052 GO:0031261 GO:0031298 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031570 GO:0031573 GO:0031935 GO:0031938 GO:0031974 GO:0031981 GO:0032392 GO:0032508 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0033260 GO:0033262 GO:0033554 GO:0034622 GO:0034641 GO:0034645 GO:0035556 GO:0036387 GO:0036388 GO:0042770 GO:0043138 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0043596 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044773 GO:0044774 GO:0044786 GO:0045786 GO:0045787 GO:0045930 GO:0045931 GO:0045934 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051128 GO:0051171 GO:0051172 GO:0051252 GO:0051276 GO:0051716 GO:0051726 GO:0060255 GO:0060968 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0071103 GO:0071163 GO:0071704 GO:0071824 GO:0071840 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072428 GO:0080090 GO:0090068 GO:0090304 GO:0090329 GO:0097159 GO:0140097 GO:1900087 GO:1901360 GO:1901363 GO:1901576 GO:1901987 GO:1901989 GO:1901990 GO:1901992 GO:1902275 GO:1902292 GO:1902299 GO:1902315 GO:1902402 GO:1902403 GO:1902576 GO:1902806 GO:1902808 GO:1902969 GO:1902975 GO:1902977 GO:1903047 GO:1903463 GO:1903464 GO:1903506 GO:1990837 GO:2000045 GO:2000104 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 map04110 map04111 map04113
Pfam Domains
Protein Families
CDC45

Protein Analysis

595

Amino Acids

67.06

Weight (kDa)

5.98

Isoelectric Point (pI)

44.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC45 PF02724 27 - 593 8.8e-161 CDC45
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016184)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25100
fragaria_vesca FvH4_2g18640
malus_domestica MD05G1172300.v1.1 MD10G1160500.v1.1
prunus_persica Prupe.8G199700_v2.0.a1 Prupe.8G199700_v2.0.a1
pyrus_communis pycom05g15890 pycom10g13870
rosa_chinensis RchiOBHm_Chr6g0284201
rosa_laevigata RLG00000012757
rosa_multiflora Rmu_sc0003778.1_g000001
rosa_roxburghii Rroxscaffold_7G00184790
rosa_rugosa Rorug06G0161800
rosa_samantha Rh6BG275200
rosa_wichuraiana Rw6G023630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 786, 1485
AccB1I GGYRCC 2 cut(s) 786, 1485
AccII CGCG 2 cut(s) 45, 316
AciI CCGC 4 cut(s) 43, 419, 797, 1183
AclWI GGATC 2 cut(s) 759, 1736
AcoI YGGCCR 1 cut(s) 341
AcsI RAATTY 4 cut(s) 835, 949, 1436, 1648
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 7 cut(s) 164, 206, 649, 762, 788, 1368, 1487
AfiI CCNNNNNNNGG 4 cut(s) 49, 796, 1148, 1631
AflIII ACRYGT 1 cut(s) 136
AgsI TTSAA 9 cut(s) 146, 475, 625, 1100, 1130, 1203, 1250, 1321, 1457
AhdI GACNNNNNGTC 1 cut(s) 1312
AjnI CCWGG 3 cut(s) 789, 1491, 1734
AjuI GAANNNNNNNTTGG 2 cut(s) 1065, 1097
AlwI GGATC 2 cut(s) 759, 1736
Ama87I CYCGRG 2 cut(s) 34, 1142
AoxI GGCC 3 cut(s) 212, 341, 426
ApeKI GCWGC 7 cut(s) 292, 794, 814, 886, 1214, 1280, 1676
ApoI RAATTY 4 cut(s) 835, 949, 1436, 1648
Asp700I GAANNNNTTC 1 cut(s) 953
Asp718I GGTACC 2 cut(s) 786, 1485
AspLEI GCGC 2 cut(s) 316, 1060
AspS9I GGNCC 4 cut(s) 157, 212, 217, 280
AsuC2I CCSGG 2 cut(s) 278, 602
AsuHPI GGTGA 3 cut(s) 266, 868, 1459
AsuII TTCGAA 1 cut(s) 1669
AvaI CYCGRG 2 cut(s) 34, 1142
AvaII GGWCC 3 cut(s) 157, 217, 280
AxyI CCTNAGG 1 cut(s) 284
BaeGI GKGCMC 1 cut(s) 121
BaeI ACNNNNGTAYC 2 cut(s) 154, 187
BanI GGYRCC 2 cut(s) 786, 1485
BarI GAAGNNNNNNTAC 4 cut(s) 617, 620, 649, 652
BauI CACGAG 2 cut(s) 166, 764
BbsI GAAGAC 5 cut(s) 174, 411, 603, 1004, 1713
BbvI GCAGC 7 cut(s) 279, 781, 826, 873, 1226, 1267, 1688
BccI CCATC 9 cut(s) 97, 488, 506, 548, 554, 863, 1042, 1262, 1516
BcgI CGANNNNNNTGC 8 cut(s) 334, 359, 368, 393, 703, 737, 834, 868
BciT130I CCWGG 3 cut(s) 791, 1493, 1736
BclI TGATCA 2 cut(s) 258, 1599
BcnI CCSGG 2 cut(s) 278, 602
BfaI CTAG 2 cut(s) 900, 1548
BfmI CTRYAG 3 cut(s) 1464, 1674, 1692
BisI GCNGC 8 cut(s) 293, 795, 815, 887, 1184, 1215, 1281, 1677
BlpI GCTNAGC 1 cut(s) 1284
BlsI GCNGC 8 cut(s) 294, 796, 816, 888, 1185, 1216, 1282, 1678
Bme1390I CCNGG 5 cut(s) 278, 602, 791, 1493, 1736
Bme18I GGWCC 3 cut(s) 157, 217, 280
BmeRI GACNNNNNGTC 1 cut(s) 1312
BmeT110I CYCGRG 2 cut(s) 34, 1142
BmgT120I GGNCC 4 cut(s) 157, 212, 217, 280
BmiI GGNNCC 6 cut(s) 41, 281, 788, 1187, 1273, 1487
BmrFI CCNGG 5 cut(s) 278, 602, 791, 1493, 1736
BmrI ACTGGG 2 cut(s) 885, 1640
BmsI GCATC 2 cut(s) 1459, 1516
BmuI ACTGGG 2 cut(s) 885, 1640
BpiI GAAGAC 5 cut(s) 174, 411, 603, 1004, 1713
BpmI CTGGAG 1 cut(s) 1013
Bpu10I CCTNAGC 1 cut(s) 1680
Bpu1102I GCTNAGC 1 cut(s) 1284
Bpu14I TTCGAA 1 cut(s) 1669
BpuEI CTTGAG 2 cut(s) 830, 1731
BpuMI CCSGG 2 cut(s) 278, 602
BsaJI CCNNGG 3 cut(s) 309, 1189, 1491
BsaWI WCCGGW 3 cut(s) 159, 175, 1170
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 796, 1148, 1631
Bse1I ACTGG 2 cut(s) 891, 1635
Bse21I CCTNAGG 1 cut(s) 284
Bse3DI GCAATG 1 cut(s) 981
BseBI CCWGG 3 cut(s) 791, 1493, 1736
BseDI CCNNGG 3 cut(s) 309, 1189, 1491
BseGI GGATG 8 cut(s) 448, 547, 559, 565, 1053, 1119, 1531, 1731
BseLI CCNNNNNNNGG 4 cut(s) 49, 796, 1148, 1631
BseMI GCAATG 1 cut(s) 981
BseMII CTCAG 4 cut(s) 275, 353, 579, 1694
BseNI ACTGG 2 cut(s) 891, 1635
BseRI GAGGAG 3 cut(s) 63, 301, 551
BseSI GKGCMC 1 cut(s) 121
BseX3I CGGCCG 1 cut(s) 341
BseXI GCAGC 7 cut(s) 279, 781, 826, 873, 1226, 1267, 1688
BseYI CCCAGC 1 cut(s) 548
Bsh1236I CGCG 2 cut(s) 45, 316
Bsh1285I CGRYCG 1 cut(s) 344
BshFI GGCC 3 cut(s) 214, 343, 428
BshNI GGYRCC 2 cut(s) 786, 1485
BsiEI CGRYCG 1 cut(s) 344
BsiHKCI CYCGRG 2 cut(s) 34, 1142
BsiSI CCGG 6 cut(s) 160, 176, 215, 277, 602, 1171
BslFI GGGAC 3 cut(s) 289, 293, 886
BslI CCNNNNNNNGG 4 cut(s) 49, 796, 1148, 1631
BsmFI GGGAC 3 cut(s) 289, 293, 886
BsmI GAATGC 1 cut(s) 1353
BsnI GGCC 3 cut(s) 214, 343, 428
BsoBI CYCGRG 2 cut(s) 34, 1142
Bsp119I TTCGAA 1 cut(s) 1669
Bsp1286I GDGCHC 1 cut(s) 121
Bsp1407I TGTACA 1 cut(s) 760
Bsp143I GATC 7 cut(s) 258, 376, 685, 751, 1120, 1599, 1728
Bsp1720I GCTNAGC 1 cut(s) 1284
BspACI CCGC 4 cut(s) 43, 419, 797, 1183
BspANI GGCC 3 cut(s) 214, 343, 428
BspCNI CTCAG 4 cut(s) 276, 354, 580, 1693
BspFNI CGCG 2 cut(s) 45, 316
BspHI TCATGA 1 cut(s) 1089
BspLI GGNNCC 6 cut(s) 41, 281, 788, 1187, 1273, 1487
BspMAI CTGCAG 2 cut(s) 1678, 1696
BspPI GGATC 2 cut(s) 759, 1736
BspT104I TTCGAA 1 cut(s) 1669
BspT107I GGYRCC 2 cut(s) 786, 1485
BsrDI GCAATG 1 cut(s) 981
BsrGI TGTACA 1 cut(s) 760
BsrI ACTGG 2 cut(s) 891, 1635
BssECI CCNNGG 3 cut(s) 309, 1189, 1491
BssMI GATC 7 cut(s) 258, 376, 685, 751, 1120, 1599, 1728
BssSI CACGAG 2 cut(s) 166, 764
Bst2BI CACGAG 2 cut(s) 166, 764
Bst2UI CCWGG 3 cut(s) 791, 1493, 1736
Bst4CI ACNGT 7 cut(s) 19, 335, 827, 1134, 1193, 1314, 1479
BstAUI TGTACA 1 cut(s) 760
BstBI TTCGAA 1 cut(s) 1669
BstC8I GCNNGC 2 cut(s) 417, 1573
BstDEI CTNAG 5 cut(s) 284, 362, 588, 1284, 1680
BstDSI CCRYGG 2 cut(s) 309, 1189
BstF5I GGATG 8 cut(s) 448, 547, 559, 565, 1053, 1119, 1531, 1731
BstFNI CGCG 2 cut(s) 45, 316
BstHHI GCGC 2 cut(s) 316, 1060
BstKTI GATC 7 cut(s) 261, 379, 688, 754, 1123, 1602, 1731
BstMBI GATC 7 cut(s) 258, 376, 685, 751, 1120, 1599, 1728
BstMCI CGRYCG 1 cut(s) 344
BstMWI GCNNNNNNNGC 5 cut(s) 425, 814, 1307, 1577, 1586
BstNI CCWGG 3 cut(s) 791, 1493, 1736
BstNSI RCATGY 1 cut(s) 140
BstSCI CCNGG 5 cut(s) 276, 600, 789, 1491, 1734
BstSFI CTRYAG 3 cut(s) 1464, 1674, 1692
BstSLI GKGCMC 1 cut(s) 121
BstUI CGCG 2 cut(s) 45, 316
BstV1I GCAGC 7 cut(s) 279, 781, 826, 873, 1226, 1267, 1688
BstV2I GAAGAC 5 cut(s) 174, 411, 603, 1004, 1713
BstXI CCANNNNNNTGG 1 cut(s) 1196
BstZI CGGCCG 1 cut(s) 341
Bsu36I CCTNAGG 1 cut(s) 284
BsuRI GGCC 3 cut(s) 214, 343, 428
BtgI CCRYGG 2 cut(s) 309, 1189
BtsCI GGATG 8 cut(s) 448, 547, 559, 565, 1053, 1119, 1531, 1731
BtsI GCAGTG 1 cut(s) 852
BtsIMutI CAGTG 4 cut(s) 24, 852, 1155, 1310
Cac8I GCNNGC 2 cut(s) 417, 1573
CciI TCATGA 1 cut(s) 1089
CfoI GCGC 2 cut(s) 316, 1060
Cfr13I GGNCC 4 cut(s) 157, 212, 217, 280
CpoI CGGWCCG 1 cut(s) 157
CseI GACGC 1 cut(s) 1316
Csp6I GTAC 7 cut(s) 163, 205, 648, 761, 787, 1367, 1486
CspI CGGWCCG 1 cut(s) 157
CviAII CATG 7 cut(s) 137, 656, 964, 1002, 1090, 1706, 1725
CviQI GTAC 7 cut(s) 163, 205, 648, 761, 787, 1367, 1486
DdeI CTNAG 5 cut(s) 284, 362, 588, 1284, 1680
DpnI GATC 7 cut(s) 260, 378, 687, 753, 1122, 1601, 1730
DpnII GATC 7 cut(s) 258, 376, 685, 751, 1120, 1599, 1728
DriI GACNNNNNGTC 1 cut(s) 1312
EaeI YGGCCR 1 cut(s) 341
EagI CGGCCG 1 cut(s) 341
Eam1105I GACNNNNNGTC 1 cut(s) 1312
EciI GGCGGA 1 cut(s) 434
EclXI CGGCCG 1 cut(s) 341
Eco47I GGWCC 3 cut(s) 157, 217, 280
Eco52I CGGCCG 1 cut(s) 341
Eco57I CTGAAG 1 cut(s) 81
Eco81I CCTNAGG 1 cut(s) 284
Eco88I CYCGRG 2 cut(s) 34, 1142
EcoO109I RGGNCCY 1 cut(s) 280
EcoRII CCWGG 3 cut(s) 789, 1491, 1734
FaeI CATG 7 cut(s) 140, 659, 967, 1005, 1093, 1709, 1728
FaqI GGGAC 3 cut(s) 289, 293, 886
FatI CATG 7 cut(s) 136, 655, 963, 1001, 1089, 1705, 1724
FauNDI CATATG 1 cut(s) 910
FbaI TGATCA 2 cut(s) 258, 1599
Fnu4HI GCNGC 8 cut(s) 293, 795, 815, 887, 1184, 1215, 1281, 1677
FokI GGATG 8 cut(s) 455, 554, 566, 572, 1060, 1126, 1538, 1718
Fsp4HI GCNGC 8 cut(s) 293, 795, 815, 887, 1184, 1215, 1281, 1677
FspBI CTAG 2 cut(s) 900, 1548
GlaI GCGC 2 cut(s) 315, 1059
GluI GCNGC 8 cut(s) 293, 795, 815, 887, 1184, 1215, 1281, 1677
GsaI CCCAGC 1 cut(s) 552
GsuI CTGGAG 1 cut(s) 1013
HaeIII GGCC 3 cut(s) 214, 343, 428
HapII CCGG 6 cut(s) 160, 176, 215, 277, 602, 1171
HgaI GACGC 1 cut(s) 1316
HhaI GCGC 2 cut(s) 316, 1060
Hin1II CATG 7 cut(s) 140, 659, 967, 1005, 1093, 1709, 1728
Hin6I GCGC 2 cut(s) 314, 1058
HinP1I GCGC 2 cut(s) 314, 1058
HincII GTYRAC 2 cut(s) 774, 1773
HindII GTYRAC 2 cut(s) 774, 1773
HindIII AAGCTT 1 cut(s) 608
HpaI GTTAAC 1 cut(s) 774
HpaII CCGG 6 cut(s) 160, 176, 215, 277, 602, 1171
HphI GGTGA 3 cut(s) 266, 868, 1459
Hpy166II GTNNAC 5 cut(s) 106, 331, 763, 774, 1773
Hpy188I TCNGA 9 cut(s) 100, 151, 235, 243, 363, 505, 589, 1267, 1533
Hpy188III TCNNGA 5 cut(s) 191, 900, 1090, 1328, 1682
Hpy8I GTNNAC 5 cut(s) 106, 331, 763, 774, 1773
Hpy99I CGWCG 3 cut(s) 67, 232, 235
HpyAV CCTTC 4 cut(s) 37, 675, 1425, 1534
HpyCH4III ACNGT 7 cut(s) 19, 335, 827, 1134, 1193, 1314, 1479
HpyCH4V TGCA 9 cut(s) 355, 908, 940, 986, 1043, 1351, 1664, 1676, 1694
HpyF10VI GCNNNNNNNGC 5 cut(s) 425, 814, 1307, 1577, 1586
HpyF3I CTNAG 5 cut(s) 284, 362, 588, 1284, 1680
Hsp92II CATG 7 cut(s) 140, 659, 967, 1005, 1093, 1709, 1728
HspAI GCGC 2 cut(s) 314, 1058
KpnI GGTACC 2 cut(s) 790, 1489
Ksp22I TGATCA 2 cut(s) 258, 1599
KspAI GTTAAC 1 cut(s) 774
Kzo9I GATC 7 cut(s) 258, 376, 685, 751, 1120, 1599, 1728
LmnI GCTCC 7 cut(s) 45, 805, 1032, 1173, 1191, 1544, 1634
Lsp1109I GCAGC 7 cut(s) 279, 781, 826, 873, 1226, 1267, 1688
LweI GCATC 2 cut(s) 1459, 1516
MaeI CTAG 2 cut(s) 900, 1548
MaeIII GTNAC 8 cut(s) 272, 467, 690, 847, 856, 1007, 1234, 1402
MalI GATC 7 cut(s) 260, 378, 687, 753, 1122, 1601, 1730
MboI GATC 7 cut(s) 258, 376, 685, 751, 1120, 1599, 1728
MfeI CAATTG 2 cut(s) 262, 1377
MhlI GDGCHC 1 cut(s) 121
MlyI GAGTC 7 cut(s) 32, 101, 509, 853, 1146, 1271, 1727
MmeI TCCRAC 2 cut(s) 213, 483
MroXI GAANNNNTTC 1 cut(s) 953
MseI TTAA 6 cut(s) 606, 746, 773, 822, 864, 1641
MslI CAYNNNNRTG 1 cut(s) 1194
MspI CCGG 6 cut(s) 160, 176, 215, 277, 602, 1171
MspR9I CCNGG 5 cut(s) 278, 602, 791, 1493, 1736
MunI CAATTG 2 cut(s) 262, 1377
Mva1269I GAATGC 1 cut(s) 1353
MvaI CCWGG 3 cut(s) 791, 1493, 1736
MvnI CGCG 2 cut(s) 45, 316
MwoI GCNNNNNNNGC 5 cut(s) 425, 814, 1307, 1577, 1586
NciI CCSGG 2 cut(s) 278, 602
NdeI CATATG 1 cut(s) 910
NdeII GATC 7 cut(s) 258, 376, 685, 751, 1120, 1599, 1728
NlaIII CATG 7 cut(s) 140, 659, 967, 1005, 1093, 1709, 1728
NlaIV GGNNCC 6 cut(s) 41, 281, 788, 1187, 1273, 1487
NmuCI GTSAC 6 cut(s) 272, 467, 690, 847, 856, 1007
NspI RCATGY 1 cut(s) 140
NspV TTCGAA 1 cut(s) 1669
PaeR7I CTCGAG 1 cut(s) 34
PagI TCATGA 1 cut(s) 1089
PciI ACATGT 1 cut(s) 136
PcsI WCGNNNNNNNCGW 1 cut(s) 239
PctI GAATGC 1 cut(s) 1353
PdmI GAANNNNTTC 1 cut(s) 953
PfoI TCCNGGA 1 cut(s) 1734
PkrI GCNGC 8 cut(s) 294, 796, 816, 888, 1185, 1216, 1282, 1678
PleI GAGTC 7 cut(s) 31, 101, 508, 853, 1146, 1270, 1726
PpsI GAGTC 7 cut(s) 31, 101, 508, 853, 1146, 1270, 1726
PpuMI RGGWCCY 1 cut(s) 280
PscI ACATGT 1 cut(s) 136
Psp5II RGGWCCY 1 cut(s) 280
Psp6I CCWGG 3 cut(s) 789, 1491, 1734
PspFI CCCAGC 1 cut(s) 548
PspGI CCWGG 3 cut(s) 789, 1491, 1734
PspN4I GGNNCC 6 cut(s) 41, 281, 788, 1187, 1273, 1487
PspPI GGNCC 4 cut(s) 157, 212, 217, 280
PspPPI RGGWCCY 1 cut(s) 280
PspXI VCTCGAGB 1 cut(s) 34
PsrI GAACNNNNNNTAC 2 cut(s) 1122, 1154
PstI CTGCAG 2 cut(s) 1678, 1696
RsaI GTAC 7 cut(s) 164, 206, 649, 762, 788, 1368, 1487
RsaNI GTAC 7 cut(s) 163, 205, 648, 761, 787, 1367, 1486
RseI CAYNNNNRTG 1 cut(s) 1194
Rsr2I CGGWCCG 1 cut(s) 157
RsrII CGGWCCG 1 cut(s) 157
SaqAI TTAA 6 cut(s) 606, 746, 773, 822, 864, 1641
SatI GCNGC 8 cut(s) 293, 795, 815, 887, 1184, 1215, 1281, 1677
Sau3AI GATC 7 cut(s) 258, 376, 685, 751, 1120, 1599, 1728
Sau96I GGNCC 4 cut(s) 157, 212, 217, 280
SchI GAGTC 7 cut(s) 32, 101, 509, 853, 1146, 1271, 1727
ScrFI CCNGG 5 cut(s) 278, 602, 791, 1493, 1736
SduI GDGCHC 1 cut(s) 121
SfaNI GCATC 2 cut(s) 1459, 1516
SfcI CTRYAG 3 cut(s) 1464, 1674, 1692
Sfr274I CTCGAG 1 cut(s) 34
SfuI TTCGAA 1 cut(s) 1669
SinI GGWCC 3 cut(s) 157, 217, 280
SlaI CTCGAG 1 cut(s) 34
SmiMI CAYNNNNRTG 1 cut(s) 1194
SmlI CTYRAG 3 cut(s) 34, 809, 1746
SmoI CTYRAG 3 cut(s) 34, 809, 1746
SsiI CCGC 4 cut(s) 43, 419, 797, 1183
SspMI CTAG 2 cut(s) 900, 1548
StyD4I CCNGG 5 cut(s) 276, 600, 789, 1491, 1734
TaaI ACNGT 7 cut(s) 19, 335, 827, 1134, 1193, 1314, 1479
TaqI TCGA 6 cut(s) 35, 566, 925, 957, 1627, 1669
TaqII GACCGA 1 cut(s) 145
TatI WGTACW 1 cut(s) 760
TauI GCSGC 1 cut(s) 1186
Tru1I TTAA 6 cut(s) 606, 746, 773, 822, 864, 1641
Tru9I TTAA 6 cut(s) 606, 746, 773, 822, 864, 1641
TscAI CASTG 4 cut(s) 24, 852, 1162, 1317
TseFI GTSAC 6 cut(s) 272, 467, 690, 847, 856, 1007
TseI GCWGC 7 cut(s) 292, 794, 814, 886, 1214, 1280, 1676
Tsp45I GTSAC 6 cut(s) 272, 467, 690, 847, 856, 1007
TspGWI ACGGA 2 cut(s) 764, 1029
TspRI CASTG 4 cut(s) 24, 852, 1162, 1317
VpaK11BI GGWCC 3 cut(s) 157, 217, 280
XapI RAATTY 4 cut(s) 835, 949, 1436, 1648
XbaI TCTAGA 1 cut(s) 899
XceI RCATGY 1 cut(s) 140
XhoI CTCGAG 1 cut(s) 34
XmnI GAANNNNTTC 1 cut(s) 953
XspI CTAG 2 cut(s) 900, 1548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.