RLG00000013827
NAC Family

inactive poly ADP-ribose polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0018534
Physical Location & Seq
Forward (+)
42200306 .. 42201761
1456 bp
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UTR
Exon/CDS
Intron
RLM00000013827

Sequence Viewer

Length: 477 bp
ATGGAGAGGCAGTCTTGCAGACCAAGGGGTTGGTCATTCCGTCTCCGAGCTAGCCGTACAAGAAGATCGAGATGTACTCGTCGGCCTTTTATGCTACTTCTACTGCAGGAGGAATCCACAGCTGTGTCTTACTCACATGGCTGTACTTATCTTGACCTCATCCAGTGCAATATGCAGGGGAGGCATCCTAGGCCAGCTTTAGAATATGAGAAATCACAGGAAAAGCCTGTTGAAATTGGCTCAAGCTCTTTGACAACTCCAAAATCCCTATGGATGCCATTTAAAGCGCTATTTGAAGCTATTTCAAAAGAAGTTGCTCCAAAAGATATGAAATTGGTTAATGTTCACTATGATCTATTCAGGTGCAAGAAGATCAGCCGGGATGGTTTTATTCAGAAGTTAAGAGCAATTGTTGGAGATGCGTTGCTGAGGTCAACCATAATGAGCCTTCAGTGCAAGGTAATGTTGTCCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PARP RST

Protein Analysis

159

Amino Acids

18.31

Weight (kDa)

9.99

Isoelectric Point (pI)

70.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RST PF12174 87 - 151 1.1e-26 RCD1-SRO-TAF4 (RST) plant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018534)

Species Orthologous Gene IDs
pyrus_communis pycom07g04710
rosa_chinensis RchiOBHm_Chr2g0176171
rosa_laevigata RLG00000011568 RLG00000013827 RLG00000018612 RLG00000026517 RLG00000032416 RLG00000033785
rosa_multiflora Rmu_sc0006800.1_g000008
rosa_roxburghii Rroxscaffold_7G00196410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 434
AfaI GTAC 3 cut(s) 58, 76, 145
AfeI AGCGCT 1 cut(s) 288
AgsI TTSAA 3 cut(s) 233, 296, 306
AleI CACNNNNGTG 1 cut(s) 122
AluBI AGCT 5 cut(s) 50, 122, 197, 246, 299
AluI AGCT 5 cut(s) 50, 122, 197, 246, 299
Alw26I GTCTC 1 cut(s) 47
Aor51HI AGCGCT 1 cut(s) 288
AoxI GGCC 2 cut(s) 83, 191
AspA2I CCTAGG 1 cut(s) 188
AspLEI GCGC 1 cut(s) 289
AsuC2I CCSGG 1 cut(s) 380
AsuNHI GCTAGC 1 cut(s) 50
AvrII CCTAGG 1 cut(s) 188
BbvCI CCTCAGC 1 cut(s) 428
BccI CCATC 1 cut(s) 377
BceAI ACGGC 1 cut(s) 39
BcnI CCSGG 1 cut(s) 380
BcoDI GTCTC 1 cut(s) 47
BfaI CTAG 2 cut(s) 51, 189
BfmI CTRYAG 1 cut(s) 104
BfoI RGCGCY 1 cut(s) 290
BlnI CCTAGG 1 cut(s) 188
Bme1390I CCNGG 1 cut(s) 380
BmrFI CCNGG 1 cut(s) 380
BmsI GCATC 3 cut(s) 193, 264, 409
BmtI GCTAGC 1 cut(s) 54
BplI GAGNNNNNCTC 2 cut(s) 61, 93
Bpu10I CCTNAGC 1 cut(s) 428
BpuEI CTTGAG 1 cut(s) 226
BpuMI CCSGG 1 cut(s) 380
BsaJI CCNNGG 2 cut(s) 23, 188
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 163
BseDI CCNNGG 2 cut(s) 23, 188
BseGI GGATG 4 cut(s) 159, 184, 279, 388
BseMII CTCAG 1 cut(s) 419
BseNI ACTGG 1 cut(s) 163
BshFI GGCC 2 cut(s) 85, 193
BsiSI CCGG 1 cut(s) 379
BsmAI GTCTC 1 cut(s) 47
BsmBI CGTCTC 1 cut(s) 47
BsnI GGCC 2 cut(s) 85, 193
Bsp143I GATC 3 cut(s) 65, 352, 372
BspANI GGCC 2 cut(s) 85, 193
BspCNI CTCAG 1 cut(s) 420
BspMAI CTGCAG 1 cut(s) 108
BspOI GCTAGC 1 cut(s) 54
BsrI ACTGG 1 cut(s) 163
BssECI CCNNGG 2 cut(s) 23, 188
BssMI GATC 3 cut(s) 65, 352, 372
BssT1I CCWWGG 2 cut(s) 23, 188
BstC8I GCNNGC 2 cut(s) 52, 195
BstDEI CTNAG 1 cut(s) 428
BstF5I GGATG 4 cut(s) 159, 184, 279, 388
BstH2I RGCGCY 1 cut(s) 290
BstHHI GCGC 1 cut(s) 289
BstKTI GATC 3 cut(s) 68, 355, 375
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 3 cut(s) 65, 352, 372
BstMWI GCNNNNNNNGC 4 cut(s) 91, 181, 190, 453
BstSCI CCNGG 1 cut(s) 378
BstSFI CTRYAG 1 cut(s) 104
BstXI CCANNNNNNTGG 1 cut(s) 30
BsuRI GGCC 2 cut(s) 85, 193
BtsCI GGATG 4 cut(s) 159, 184, 279, 388
BtsIMutI CAGTG 2 cut(s) 170, 458
Cac8I GCNNGC 2 cut(s) 52, 195
CfoI GCGC 1 cut(s) 289
Csp6I GTAC 3 cut(s) 57, 75, 144
CviAII CATG 1 cut(s) 137
CviQI GTAC 3 cut(s) 57, 75, 144
DdeI CTNAG 1 cut(s) 428
DpnI GATC 3 cut(s) 67, 354, 374
DpnII GATC 3 cut(s) 65, 352, 372
DraI TTTAAA 1 cut(s) 283
Eco130I CCWWGG 2 cut(s) 23, 188
Eco47III AGCGCT 1 cut(s) 288
Eco57I CTGAAG 1 cut(s) 434
EcoT14I CCWWGG 2 cut(s) 23, 188
ErhI CCWWGG 2 cut(s) 23, 188
Esp3I CGTCTC 1 cut(s) 47
FaeI CATG 1 cut(s) 140
FatI CATG 1 cut(s) 136
FauNDI CATATG 1 cut(s) 473
FokI GGATG 4 cut(s) 146, 171, 286, 395
FspBI CTAG 2 cut(s) 51, 189
GlaI GCGC 1 cut(s) 288
HaeII RGCGCY 1 cut(s) 290
HaeIII GGCC 2 cut(s) 85, 193
HapII CCGG 1 cut(s) 379
HhaI GCGC 1 cut(s) 289
Hin1II CATG 1 cut(s) 140
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HincII GTYRAC 1 cut(s) 435
HindII GTYRAC 1 cut(s) 435
HinfI GANTC 1 cut(s) 113
HpaII CCGG 1 cut(s) 379
Hpy166II GTNNAC 2 cut(s) 346, 435
Hpy188I TCNGA 2 cut(s) 47, 396
Hpy188III TCNNGA 2 cut(s) 69, 152
Hpy8I GTNNAC 2 cut(s) 346, 435
Hpy99I CGWCG 1 cut(s) 84
HpyAV CCTTC 1 cut(s) 458
HpyCH4V TGCA 6 cut(s) 18, 106, 168, 175, 366, 456
HpyF10VI GCNNNNNNNGC 4 cut(s) 91, 181, 190, 453
HpyF3I CTNAG 1 cut(s) 428
Hsp92II CATG 1 cut(s) 140
HspAI GCGC 1 cut(s) 287
Kzo9I GATC 3 cut(s) 65, 352, 372
LmnI GCTCC 1 cut(s) 322
LpnPI CCDG 8 cut(s) 92, 161, 176, 203, 207, 240, 346, 392
LweI GCATC 3 cut(s) 193, 264, 409
MaeI CTAG 2 cut(s) 51, 189
MalI GATC 3 cut(s) 67, 354, 374
MboI GATC 3 cut(s) 65, 352, 372
MboII GAAGA 2 cut(s) 75, 382
MfeI CAATTG 1 cut(s) 408
MluCI AATT 3 cut(s) 234, 332, 408
MmeI TCCRAC 1 cut(s) 394
MnlI CCTC 4 cut(s) 103, 167, 174, 423
MseI TTAA 3 cut(s) 282, 339, 401
MslI CAYNNNNRTG 1 cut(s) 122
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 1 cut(s) 379
MspR9I CCNGG 1 cut(s) 380
MunI CAATTG 1 cut(s) 408
MwoI GCNNNNNNNGC 4 cut(s) 91, 181, 190, 453
NciI CCSGG 1 cut(s) 380
NdeI CATATG 1 cut(s) 473
NdeII GATC 3 cut(s) 65, 352, 372
NheI GCTAGC 1 cut(s) 50
NlaIII CATG 1 cut(s) 140
OliI CACNNNNGTG 1 cut(s) 122
PfeI GAWTC 1 cut(s) 113
PstI CTGCAG 1 cut(s) 108
PvuII CAGCTG 1 cut(s) 122
RsaI GTAC 3 cut(s) 58, 76, 145
RsaNI GTAC 3 cut(s) 57, 75, 144
RseI CAYNNNNRTG 1 cut(s) 122
SaqAI TTAA 3 cut(s) 282, 339, 401
Sau3AI GATC 3 cut(s) 65, 352, 372
ScrFI CCNGG 1 cut(s) 380
SetI ASST 9 cut(s) 52, 124, 159, 199, 248, 301, 365, 434, 462
SfaNI GCATC 3 cut(s) 193, 264, 409
SfcI CTRYAG 1 cut(s) 104
SmiMI CAYNNNNRTG 1 cut(s) 122
SmlI CTYRAG 1 cut(s) 241
SmoI CTYRAG 1 cut(s) 241
Sse9I AATT 3 cut(s) 234, 332, 408
SspMI CTAG 2 cut(s) 51, 189
StyD4I CCNGG 1 cut(s) 378
StyI CCWWGG 2 cut(s) 23, 188
TaqI TCGA 1 cut(s) 68
TasI AATT 3 cut(s) 234, 332, 408
TatI WGTACW 2 cut(s) 74, 143
TfiI GAWTC 1 cut(s) 113
Tru1I TTAA 3 cut(s) 282, 339, 401
Tru9I TTAA 3 cut(s) 282, 339, 401
TscAI CASTG 2 cut(s) 170, 458
TspDTI ATGAA 1 cut(s) 344
TspGWI ACGGA 1 cut(s) 29
TspRI CASTG 2 cut(s) 170, 458
XcmI CCANNNNNNNNNTGG 1 cut(s) 267
XmaJI CCTAGG 1 cut(s) 188
XspI CTAG 2 cut(s) 51, 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.