RLG00000013912

tRNA-dihydrouridine(47) synthase NAD(P)( )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0019954
Physical Location & Seq
Reverse (-)
44183222 .. 44184097
876 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013912

Sequence Viewer

Length: 426 bp
ATGGGCGACGACTCTCCTGCCGCAACTGGGCCGGGCCCAGAGGTCTCCAACTGCGCGGCGGCGGCGGCTGAGAGAACGCCACCTTGCTCTAAGGATCAAAACGACGCCGTCTGTGAGGGAAACGCGAAGCCTGCACAGTCGAACGTAGTGAAGGAGAAGAAGTCCAAGCGGCAGCTCAAGCGTGAACGCCGCGAGGTTATAAATCGATCGCCCCTCGGTATCTGTCCAGAGGTAGTAAAGAAAGGAGATGTTAGTCGGTGTCCATATAATGATAAGTGCCGCTACAGCCACGACATAGAAGCCTATAAGGCTCAGAAACCTGAGGACATAGAGGGAGAATGTCCTTTCATGATGAGCCATGCCCCTATGGTTTGTCATGTAGGTTCTTGGGTACACATAGCAATGGTGTTGCAGATGGAGCTGTGA

Protein Analysis

142

Amino Acids

15.48

Weight (kDa)

6.88

Isoelectric Point (pI)

62.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH_DUS3L PF25585 74 - 105 3.3e-11 DUS3L CCCH zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019954)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0268421 RchiOBHm_Chr6g0271631
rosa_laevigata RLG00000013912
rosa_multiflora Rmu_sc0008184.1_g000008
rosa_rugosa Rorug06G0042000
rosa_samantha Rh6AG161300 Rh6BG165700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 200
AccII CGCG 3 cut(s) 56, 125, 192
AciI CCGC 8 cut(s) 21, 56, 59, 62, 65, 169, 190, 280
AclWI GGATC 1 cut(s) 102
AcyI GRCGYC 1 cut(s) 105
AfaI GTAC 1 cut(s) 393
AfiI CCNNNNNNNGG 1 cut(s) 27
AluBI AGCT 2 cut(s) 175, 421
AluI AGCT 2 cut(s) 175, 421
Alw26I GTCTC 1 cut(s) 49
AlwI GGATC 1 cut(s) 102
AoxI GGCC 2 cut(s) 29, 34
ApaI GGGCCC 1 cut(s) 38
ApeKI GCWGC 1 cut(s) 172
AspLEI GCGC 1 cut(s) 56
AspS9I GGNCC 3 cut(s) 29, 34, 35
AsuC2I CCSGG 1 cut(s) 33
AxyI CCTNAGG 1 cut(s) 321
BaeGI GKGCMC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 38
BbvI GCAGC 1 cut(s) 184
BccI CCATC 1 cut(s) 409
BceAI ACGGC 1 cut(s) 92
BcgI CGANNNNNNTGC 1 cut(s) 33
BcnI CCSGG 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 49
BfmI CTRYAG 1 cut(s) 283
BglI GCCNNNNNGGC 1 cut(s) 308
BisI GCNGC 9 cut(s) 21, 57, 60, 63, 66, 170, 173, 190, 280
BlsI GCNGC 9 cut(s) 22, 58, 61, 64, 67, 171, 174, 191, 281
Bme1390I CCNGG 1 cut(s) 33
BmgT120I GGNCC 3 cut(s) 29, 34, 35
BmiI GGNNCC 1 cut(s) 36
BmrFI CCNGG 1 cut(s) 33
BmrI ACTGGG 1 cut(s) 36
BmuI ACTGGG 1 cut(s) 36
BpuEI CTTGAG 1 cut(s) 161
BpuMI CCSGG 1 cut(s) 33
Bsa29I ATCGAT 1 cut(s) 205
BsaHI GRCGYC 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 49
BsaJI CCNNGG 1 cut(s) 214
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bsc4I CCNNNNNNNGG 1 cut(s) 27
Bse1I ACTGG 1 cut(s) 31
Bse21I CCTNAGG 1 cut(s) 321
Bse3DI GCAATG 1 cut(s) 408
BseCI ATCGAT 1 cut(s) 205
BseDI CCNNGG 1 cut(s) 214
BseLI CCNNNNNNNGG 1 cut(s) 27
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 3 cut(s) 60, 312, 326
BseNI ACTGG 1 cut(s) 31
BseSI GKGCMC 1 cut(s) 38
BseXI GCAGC 1 cut(s) 184
BsgI GTGCAG 1 cut(s) 117
Bsh1236I CGCG 3 cut(s) 56, 125, 192
Bsh1285I CGRYCG 1 cut(s) 209
BshFI GGCC 2 cut(s) 31, 36
BshVI ATCGAT 1 cut(s) 205
BsiEI CGRYCG 1 cut(s) 209
BsiSI CCGG 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 27
BsmAI GTCTC 1 cut(s) 49
BsnI GGCC 2 cut(s) 31, 36
Bso31I GGTCTC 1 cut(s) 49
Bsp120I GGGCCC 1 cut(s) 34
Bsp1286I GDGCHC 1 cut(s) 38
Bsp143I GATC 2 cut(s) 94, 206
BspACI CCGC 8 cut(s) 21, 56, 59, 62, 65, 169, 190, 280
BspANI GGCC 2 cut(s) 31, 36
BspCNI CTCAG 3 cut(s) 61, 313, 325
BspDI ATCGAT 1 cut(s) 205
BspFNI CGCG 3 cut(s) 56, 125, 192
BspHI TCATGA 1 cut(s) 348
BspLI GGNNCC 1 cut(s) 36
BspPI GGATC 1 cut(s) 102
BspTNI GGTCTC 1 cut(s) 49
BsrDI GCAATG 1 cut(s) 408
BsrI ACTGG 1 cut(s) 31
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 2 cut(s) 94, 206
BssNI GRCGYC 1 cut(s) 105
Bst4CI ACNGT 1 cut(s) 138
BstACI GRCGYC 1 cut(s) 105
BstC8I GCNNGC 1 cut(s) 132
BstDEI CTNAG 4 cut(s) 69, 90, 312, 321
BstFNI CGCG 3 cut(s) 56, 125, 192
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 2 cut(s) 97, 209
BstMAI GTCTC 1 cut(s) 49
BstMBI GATC 2 cut(s) 94, 206
BstMCI CGRYCG 1 cut(s) 209
BstMWI GCNNNNNNNGC 7 cut(s) 62, 65, 131, 178, 285, 308, 418
BstSCI CCNGG 1 cut(s) 31
BstSFI CTRYAG 1 cut(s) 283
BstSLI GKGCMC 1 cut(s) 38
BstUI CGCG 3 cut(s) 56, 125, 192
BstV1I GCAGC 1 cut(s) 184
Bsu15I ATCGAT 1 cut(s) 205
Bsu36I CCTNAGG 1 cut(s) 321
BsuRI GGCC 2 cut(s) 31, 36
BsuTUI ATCGAT 1 cut(s) 205
Cac8I GCNNGC 1 cut(s) 132
CciI TCATGA 1 cut(s) 348
CfoI GCGC 1 cut(s) 56
Cfr13I GGNCC 3 cut(s) 29, 34, 35
ClaI ATCGAT 1 cut(s) 205
CseI GACGC 1 cut(s) 113
Csp6I GTAC 1 cut(s) 392
CviAII CATG 3 cut(s) 349, 359, 377
CviQI GTAC 1 cut(s) 392
DdeI CTNAG 4 cut(s) 69, 90, 312, 321
DpnI GATC 2 cut(s) 96, 208
DpnII GATC 2 cut(s) 94, 206
Eco24I GRGCYC 1 cut(s) 38
Eco31I GGTCTC 1 cut(s) 49
Eco81I CCTNAGG 1 cut(s) 321
EcoT38I GRGCYC 1 cut(s) 38
FaeI CATG 3 cut(s) 352, 362, 380
FatI CATG 3 cut(s) 348, 358, 376
Fnu4HI GCNGC 9 cut(s) 21, 57, 60, 63, 66, 170, 173, 190, 280
FriOI GRGCYC 1 cut(s) 38
Fsp4HI GCNGC 9 cut(s) 21, 57, 60, 63, 66, 170, 173, 190, 280
GlaI GCGC 1 cut(s) 55
GluI GCNGC 9 cut(s) 21, 57, 60, 63, 66, 170, 173, 190, 280
HaeIII GGCC 2 cut(s) 31, 36
HapII CCGG 1 cut(s) 32
HgaI GACGC 1 cut(s) 113
HhaI GCGC 1 cut(s) 56
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 3 cut(s) 352, 362, 380
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HinfI GANTC 1 cut(s) 11
HpaII CCGG 1 cut(s) 32
Hpy166II GTNNAC 2 cut(s) 185, 394
Hpy188I TCNGA 1 cut(s) 315
Hpy188III TCNNGA 2 cut(s) 227, 349
Hpy8I GTNNAC 2 cut(s) 185, 394
Hpy99I CGWCG 2 cut(s) 11, 107
HpyAV CCTTC 1 cut(s) 145
HpyCH4III ACNGT 1 cut(s) 138
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 2 cut(s) 134, 412
HpyF10VI GCNNNNNNNGC 7 cut(s) 62, 65, 131, 178, 285, 308, 418
HpyF3I CTNAG 4 cut(s) 69, 90, 312, 321
HpySE526I ACGT 1 cut(s) 144
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 3 cut(s) 352, 362, 380
HspAI GCGC 1 cut(s) 54
Kzo9I GATC 2 cut(s) 94, 206
LmnI GCTCC 1 cut(s) 418
LpnPI CCDG 7 cut(s) 12, 30, 45, 51, 144, 240, 333
Lsp1109I GCAGC 1 cut(s) 184
MaeII ACGT 1 cut(s) 144
MalI GATC 2 cut(s) 96, 208
MboI GATC 2 cut(s) 94, 206
MboII GAAGA 1 cut(s) 169
MhlI GDGCHC 1 cut(s) 38
MlyI GAGTC 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 7 cut(s) 34, 109, 187, 223, 224, 316, 325
MslI CAYNNNNRTG 1 cut(s) 401
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 1 cut(s) 33
MvnI CGCG 3 cut(s) 56, 125, 192
MwoI GCNNNNNNNGC 7 cut(s) 62, 65, 131, 178, 285, 308, 418
NciI CCSGG 1 cut(s) 33
NdeII GATC 2 cut(s) 94, 206
NlaIII CATG 3 cut(s) 352, 362, 380
NlaIV GGNNCC 1 cut(s) 36
PagI TCATGA 1 cut(s) 348
PflFI GACNNNGTC 1 cut(s) 107
PkrI GCNGC 9 cut(s) 22, 58, 61, 64, 67, 171, 174, 191, 281
Ple19I CGATCG 1 cut(s) 209
PleI GAGTC 1 cut(s) 5
PpsI GAGTC 1 cut(s) 5
PsiI TTATAA 1 cut(s) 200
PspN4I GGNNCC 1 cut(s) 36
PspOMI GGGCCC 1 cut(s) 34
PspPI GGNCC 3 cut(s) 29, 34, 35
PsyI GACNNNGTC 1 cut(s) 107
PvuI CGATCG 1 cut(s) 209
RsaI GTAC 1 cut(s) 393
RsaNI GTAC 1 cut(s) 392
RseI CAYNNNNRTG 1 cut(s) 401
SatI GCNGC 9 cut(s) 21, 57, 60, 63, 66, 170, 173, 190, 280
Sau3AI GATC 2 cut(s) 94, 206
Sau96I GGNCC 3 cut(s) 29, 34, 35
SchI GAGTC 1 cut(s) 5
ScrFI CCNGG 1 cut(s) 33
SduI GDGCHC 1 cut(s) 38
SetI ASST 9 cut(s) 45, 85, 147, 177, 198, 234, 322, 385, 423
SfcI CTRYAG 1 cut(s) 283
SmiMI CAYNNNNRTG 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
SsiI CCGC 8 cut(s) 21, 56, 59, 62, 65, 169, 190, 280
StyD4I CCNGG 1 cut(s) 31
TaaI ACNGT 1 cut(s) 138
TaiI ACGT 1 cut(s) 147
TaqI TCGA 2 cut(s) 140, 205
TauI GCSGC 8 cut(s) 23, 59, 62, 65, 68, 172, 192, 282
TseI GCWGC 1 cut(s) 172
TspDTI ATGAA 1 cut(s) 337
Tth111I GACNNNGTC 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.