RLG00000014725

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0017849
Physical Location & Seq
Forward (+)
56834399 .. 56838449
4051 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014725

Sequence Viewer

Length: 525 bp
ATGGAAGGGACATCTGAGACTCCTAATTCCACTCCCATACCTACTCCAACTAATGGTTCTAGCATTGGAACAACTCCGACACCCACATCCACTCCTACTCAGACGGTGGACCAAAGTCATCAACAAGGAGCTGCAGCACTACCACCCCTTCCACCAGTTTCAAAAGATCCTGATGGAACAACCAGCGGCGTCGAGTTCAAGAAGAAAGCTCTGGAGTCTCTGCTTCAGCTTCTCGAAGACAACGACAAGGCCGTTTCCCTAGTCTTCATTTTGCACCTCCGCTGTCTCTCCCCTTGGAAACGAACTCGATCTCGAACCCGACCCTTAATCGAATTCGAACTCCAGCTGCCTCTCCAGCATTACTCCCGCTCCTTATTGATTCCGATCCTGTTTTGGCGACTCAGGACCTCAACTCCTCCGTGGTCTTTGGTCTCTGCGGCGACGGCCATTGAGCCAGCACACTGCCAATCAAAGATTAAGGCCAATGGAGAAGGCCACGTGTTCGGGTTCTTCATGCTGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

175

Amino Acids

19.06

Weight (kDa)

8.66

Isoelectric Point (pI)

51.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017849)

Species Orthologous Gene IDs
rosa_laevigata RLG00000014725
rosa_multiflora Rmu_sc0000758.1_g000010
rosa_roxburghii Rroxscaffold_7G00198470
rosa_samantha Rh2BG514900 Rh2DG274300 Rh2DG527000 Rh3AG123000 Rh4DG205600 Rh5CG206500 Rh6CG096500 Rh7AG301400
rosa_wichuraiana Rw6G023390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 53
AccBSI CCGCTC 1 cut(s) 369
AciI CCGC 4 cut(s) 186, 280, 367, 437
AclWI GGATC 2 cut(s) 161, 379
AcoI YGGCCR 1 cut(s) 444
AcsI RAATTY 1 cut(s) 332
AcuI CTGAAG 1 cut(s) 209
AcvI CACGTG 1 cut(s) 499
AcyI GRCGYC 1 cut(s) 189
AfiI CCNNNNNNNGG 1 cut(s) 53
AflIII ACRYGT 1 cut(s) 498
AgsI TTSAA 2 cut(s) 162, 199
AjuI GAANNNNNNNTTGG 2 cut(s) 40, 72
AluBI AGCT 4 cut(s) 131, 209, 229, 346
AluI AGCT 4 cut(s) 131, 209, 229, 346
Alw26I GTCTC 4 cut(s) 11, 222, 290, 436
AlwI GGATC 2 cut(s) 161, 379
AoxI GGCC 4 cut(s) 249, 444, 480, 493
ApeKI GCWGC 3 cut(s) 131, 134, 346
ApoI RAATTY 1 cut(s) 332
AspS9I GGNCC 2 cut(s) 109, 405
AsuII TTCGAA 1 cut(s) 336
AvaII GGWCC 2 cut(s) 109, 405
BbrPI CACGTG 1 cut(s) 499
BbsI GAAGAC 2 cut(s) 243, 256
BbvI GCAGC 3 cut(s) 118, 146, 333
BccI CCATC 1 cut(s) 167
BceAI ACGGC 2 cut(s) 236, 459
BcoDI GTCTC 4 cut(s) 11, 222, 290, 436
BfaI CTAG 2 cut(s) 60, 260
BfmI CTRYAG 1 cut(s) 132
BisI GCNGC 5 cut(s) 132, 135, 187, 347, 438
BlsI GCNGC 5 cut(s) 133, 136, 188, 348, 439
Bme18I GGWCC 2 cut(s) 109, 405
BmgT120I GGNCC 2 cut(s) 109, 405
BoxI GACNNNNGTC 1 cut(s) 114
BpiI GAAGAC 2 cut(s) 243, 256
BpmI CTGGAG 3 cut(s) 233, 326, 338
Bpu14I TTCGAA 1 cut(s) 336
BsaAI YACGTR 1 cut(s) 499
BsaBI GATNNNNATC 1 cut(s) 383
BsaHI GRCGYC 1 cut(s) 189
BsaI GGTCTC 1 cut(s) 436
BsaJI CCNNGG 2 cut(s) 293, 419
BsaXI ACNNNNNCTCC 6 cut(s) 76, 106, 353, 383, 397, 427
Bsc4I CCNNNNNNNGG 1 cut(s) 53
Bse1I ACTGG 1 cut(s) 155
Bse8I GATNNNNATC 1 cut(s) 383
BseDI CCNNGG 2 cut(s) 293, 419
BseGI GGATG 1 cut(s) 86
BseJI GATNNNNATC 1 cut(s) 383
BseLI CCNNNNNNNGG 1 cut(s) 53
BseMII CTCAG 3 cut(s) 6, 113, 415
BseNI ACTGG 1 cut(s) 155
BseRI GAGGAG 1 cut(s) 405
BseXI GCAGC 3 cut(s) 118, 146, 333
BshFI GGCC 4 cut(s) 251, 446, 482, 495
BslFI GGGAC 1 cut(s) 22
BslI CCNNNNNNNGG 1 cut(s) 53
BsmAI GTCTC 4 cut(s) 11, 222, 290, 436
BsmFI GGGAC 1 cut(s) 22
BsnI GGCC 4 cut(s) 251, 446, 482, 495
Bso31I GGTCTC 1 cut(s) 436
Bsp119I TTCGAA 1 cut(s) 336
Bsp143I GATC 3 cut(s) 166, 308, 384
BspACI CCGC 4 cut(s) 186, 280, 367, 437
BspANI GGCC 4 cut(s) 251, 446, 482, 495
BspCNI CTCAG 3 cut(s) 7, 112, 414
BspMAI CTGCAG 1 cut(s) 136
BspPI GGATC 2 cut(s) 161, 379
BspT104I TTCGAA 1 cut(s) 336
BspTNI GGTCTC 1 cut(s) 436
BsrBI CCGCTC 1 cut(s) 369
BsrI ACTGG 1 cut(s) 155
BssECI CCNNGG 2 cut(s) 293, 419
BssMI GATC 3 cut(s) 166, 308, 384
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 1 cut(s) 293
Bst4CI ACNGT 1 cut(s) 106
BstACI GRCGYC 1 cut(s) 189
BstBAI YACGTR 1 cut(s) 499
BstBI TTCGAA 1 cut(s) 336
BstC8I GCNNGC 1 cut(s) 456
BstDEI CTNAG 3 cut(s) 15, 99, 401
BstDSI CCRYGG 1 cut(s) 419
BstF5I GGATG 1 cut(s) 86
BstKTI GATC 3 cut(s) 169, 311, 387
BstMAI GTCTC 4 cut(s) 11, 222, 290, 436
BstMBI GATC 3 cut(s) 166, 308, 384
BstMWI GCNNNNNNNGC 2 cut(s) 355, 443
BstPAI GACNNNNGTC 1 cut(s) 114
BstSFI CTRYAG 1 cut(s) 132
BstV1I GCAGC 3 cut(s) 118, 146, 333
BstV2I GAAGAC 2 cut(s) 243, 256
BstX2I RGATCY 1 cut(s) 166
BstYI RGATCY 1 cut(s) 166
BsuRI GGCC 4 cut(s) 251, 446, 482, 495
BtgI CCRYGG 1 cut(s) 419
BtsCI GGATG 1 cut(s) 86
BtsI GCAGTG 1 cut(s) 460
BtsIMutI CAGTG 1 cut(s) 460
Cac8I GCNNGC 1 cut(s) 456
Cfr13I GGNCC 2 cut(s) 109, 405
CseI GACGC 1 cut(s) 178
CviAII CATG 1 cut(s) 514
CviJI RGCY 9 cut(s) 131, 209, 229, 251, 346, 446, 454, 482, 495
CviKI_1 RGCY 9 cut(s) 131, 209, 229, 251, 346, 446, 454, 482, 495
DdeI CTNAG 3 cut(s) 15, 99, 401
DpnI GATC 3 cut(s) 168, 310, 386
DpnII GATC 3 cut(s) 166, 308, 384
EaeI YGGCCR 1 cut(s) 444
Eco130I CCWWGG 1 cut(s) 293
Eco31I GGTCTC 1 cut(s) 436
Eco47I GGWCC 2 cut(s) 109, 405
Eco57I CTGAAG 1 cut(s) 209
Eco72I CACGTG 1 cut(s) 499
EcoO109I RGGNCCY 1 cut(s) 405
EcoRI GAATTC 1 cut(s) 332
EcoT14I CCWWGG 1 cut(s) 293
ErhI CCWWGG 1 cut(s) 293
FaeI CATG 1 cut(s) 517
FaiI YATR 2 cut(s) 38, 515
FaqI GGGAC 1 cut(s) 22
FatI CATG 1 cut(s) 513
FauI CCCGC 1 cut(s) 374
Fnu4HI GCNGC 5 cut(s) 132, 135, 187, 347, 438
FokI GGATG 1 cut(s) 73
Fsp4HI GCNGC 5 cut(s) 132, 135, 187, 347, 438
FspBI CTAG 2 cut(s) 60, 260
GluI GCNGC 5 cut(s) 132, 135, 187, 347, 438
GsuI CTGGAG 3 cut(s) 233, 326, 338
HaeIII GGCC 4 cut(s) 251, 446, 482, 495
HgaI GACGC 1 cut(s) 178
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 1 cut(s) 517
HinfI GANTC 4 cut(s) 19, 215, 379, 399
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 4 cut(s) 16, 78, 102, 384
Hpy188III TCNNGA 6 cut(s) 170, 199, 212, 233, 312, 403
Hpy8I GTNNAC 1 cut(s) 109
Hpy99I CGWCG 2 cut(s) 194, 445
HpyAV CCTTC 2 cut(s) 158, 485
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4IV ACGT 1 cut(s) 498
HpyCH4V TGCA 2 cut(s) 134, 274
HpyF10VI GCNNNNNNNGC 2 cut(s) 355, 443
HpyF3I CTNAG 3 cut(s) 15, 99, 401
HpySE526I ACGT 1 cut(s) 498
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 1 cut(s) 517
Kzo9I GATC 3 cut(s) 166, 308, 384
LmnI GCTCC 2 cut(s) 128, 374
LpnPI CCDG 9 cut(s) 168, 183, 196, 197, 356, 368, 388, 401, 468
Lsp1109I GCAGC 3 cut(s) 118, 146, 333
MaeI CTAG 2 cut(s) 60, 260
MaeII ACGT 1 cut(s) 498
MalI GATC 3 cut(s) 168, 310, 386
MbiI CCGCTC 1 cut(s) 369
MboI GATC 3 cut(s) 166, 308, 384
MboII GAAGA 4 cut(s) 214, 248, 256, 502
MflI RGATCY 1 cut(s) 166
MluCI AATT 2 cut(s) 25, 332
MlyI GAGTC 3 cut(s) 13, 224, 393
MmeI TCCRAC 2 cut(s) 71, 101
MnlI CCTC 4 cut(s) 287, 360, 418, 426
MseI TTAA 2 cut(s) 326, 477
MspA1I CMGCKG 3 cut(s) 186, 282, 346
MwoI GCNNNNNNNGC 2 cut(s) 355, 443
NdeII GATC 3 cut(s) 166, 308, 384
NlaIII CATG 1 cut(s) 517
NspV TTCGAA 1 cut(s) 336
PcsI WCGNNNNNNNCGW 2 cut(s) 240, 249
PfeI GAWTC 1 cut(s) 379
PflMI CCANNNNNTGG 1 cut(s) 53
PkrI GCNGC 5 cut(s) 133, 136, 188, 348, 439
PleI GAGTC 3 cut(s) 13, 223, 393
PmaCI CACGTG 1 cut(s) 499
PmlI CACGTG 1 cut(s) 499
PpsI GAGTC 3 cut(s) 13, 223, 393
Ppu21I YACGTR 1 cut(s) 499
PpuMI RGGWCCY 1 cut(s) 405
PshAI GACNNNNGTC 1 cut(s) 114
Psp5II RGGWCCY 1 cut(s) 405
PspCI CACGTG 1 cut(s) 499
PspPI GGNCC 2 cut(s) 109, 405
PspPPI RGGWCCY 1 cut(s) 405
PstI CTGCAG 1 cut(s) 136
PsuI RGATCY 1 cut(s) 166
PvuII CAGCTG 1 cut(s) 346
SaqAI TTAA 2 cut(s) 326, 477
SatI GCNGC 5 cut(s) 132, 135, 187, 347, 438
Sau3AI GATC 3 cut(s) 166, 308, 384
Sau96I GGNCC 2 cut(s) 109, 405
SchI GAGTC 3 cut(s) 13, 224, 393
SetI ASST 8 cut(s) 43, 133, 211, 231, 279, 348, 410, 501
SfcI CTRYAG 1 cut(s) 132
SfuI TTCGAA 1 cut(s) 336
SinI GGWCC 2 cut(s) 109, 405
Sse9I AATT 2 cut(s) 25, 332
SsiI CCGC 4 cut(s) 186, 280, 367, 437
SspMI CTAG 2 cut(s) 60, 260
StyI CCWWGG 1 cut(s) 293
TaaI ACNGT 1 cut(s) 106
TaiI ACGT 1 cut(s) 501
TaqI TCGA 6 cut(s) 192, 234, 307, 313, 330, 336
TasI AATT 2 cut(s) 25, 332
TauI GCSGC 2 cut(s) 189, 440
TfiI GAWTC 1 cut(s) 379
Tru1I TTAA 2 cut(s) 326, 477
Tru9I TTAA 2 cut(s) 326, 477
TscAI CASTG 1 cut(s) 467
TseI GCWGC 3 cut(s) 131, 134, 346
TspDTI ATGAA 2 cut(s) 256, 502
TspGWI ACGGA 1 cut(s) 408
TspRI CASTG 1 cut(s) 467
Van91I CCANNNNNTGG 1 cut(s) 53
VpaK11BI GGWCC 2 cut(s) 109, 405
XapI RAATTY 1 cut(s) 332
XspI CTAG 2 cut(s) 60, 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.