Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0014491

Physical Location & Seq

Genomic Coordinates Forward (+)

4178918 .. 4186141

Gene Structure 7224 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016141

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3219 bp

ATGCAGCGCCAGAAATCGATCCTCTCCTTCTTCCGGAAACCCTTGCAGGACAAAAGCACCTCCACCGCCGGAGATGACGGCCGTGGAGTTAATCAGTTCGCGGCGAAGGAGCAGGAGCCCGTCGGATCCAATCAGCCTCCGGATCTCGAAGTTCGAGGAACAGACACTCCGCCTGAGAAGGTGCCACGTCAGATTCTGCCGGCGAATTCTGCGAGCCTGAAAGACTCTTCGCCTTTCTCTAGTATTATGCATAAGTTTATGAAGGTCGATGACACACATAAGGCTTCTCAAAGCCACCGGAATGAACCAAATGGTGGTTCCTCTAATGATTGTACAGTATCTGGTAAACTCAATGATATCGATTCCAGTAAGAAAATTAATCGAGGTGCTGTGATGCATATCGAAAGTGATGAGGACATTGCTGGACCAGAAACACCGGGGACACAACGTCTAGTTCCGCATATGAAGCGAATTCAAGAGGAGATTCCTATGTTTGGGGATAAATTTGGGAGTTCTTTGGCAGATTCTAGCAAAAGATTGAAAGTTCTTGAGGAGCCAACAGTCTTAGACAAGAGCCATGGGGAAGCATCTGATATGGCTAGCAAGTTTGAGTGGCTTGACCCATCCCGAATCAGAGATGCAAATCGCAGAAGGCCTGGTGATCCTCTCTATGATAAAACATCACTTTTTATACCGCCTGATGCTCTGAAGAAAATGTCAGCATCACAAAGACAATACTGGGATGTCAAGTGTCAGTATATGGATGTTGTGATTTTCTTTAAAGTGGGAAAGTTTTATGAGCTTTATGAACTAGATGCTGAAATTGGCCACAAGGAGCTTGATTGGAAAATGACCTTGAGTGGTGTTGGGAAATGTCGTCAGGTTGGCATCTCTGAAAGTGGGATTGACGATGCTGTTCAAAAGTTGGTTGTCCGTGGGTATAAAGTTGGACGGATAGAGCAGCTGGAAACTTCTGATCAAGTAAAAGCCAGAGGTGCCAAAGCTGTAATTCCAAGAAAATTGGTTCAGGTAGTCACACCATCAACCACAACTGATGGAAATATTGGGCCTGATGCTGTTCATCTTCTTGCAATAAAAGAGGGCAACTCTGGGGTCGGCAATGGTTCAGTTGTATACGGGTTTGCTTTTGTTGATTGTTCTGCTTTAAAGTTCTGGATTGGTGCTATCAGTGATGATGCTTCGTGTGCTGCTTTGGGGGCTTTATTGATGCAAGTGTCGCCCAAAGAAGTTGTATATGAAAACAGAGCGCTCTCCAAAGAAGCTCAAAAAGCACTGAAAAAGTACTCAACAGGTTCCGCACTGCAATTGACTCCAGTGCAGCCAGTCAATGATTTTATGGATGCTTCTGAAGTTAGTAAATTGATTCAGTTGAAAGAATACTTCAAAGGGTCTTCGAATTTGTGGAATCATGGACCTGATGGTGTGGTACACCATGATATAACTTTACCTGCTCTGGGTACACTTATTGACCACCTATCCAGGTTGATGTTAGATGACGTTTTACGCAATGGAGATATATTACCTTACCAAGTTTATAAGGGTTGCCTCAAAATGGATGGACAGACGCTGATAAATCTTGAGATTTTCAGTAATACTGCTGATGGTGGTCCATCAGGTACGCTGTATGCATATCTCGATAACTGTGTGACTGCATCTGGTAAGCGGCTTTTGAGGAAGTGGATCTGCCATCCCTTGAAGTCTGTTGAAGCTATTAATAACAGGCTTAATGTGGTTGAAGATCTACTGGCACATCCAGAAATGGTGCCACTTATTGCTCAGTATCTCCGCAAGCTTCCAGACTTAGAGAGGTTGTTTGGACGGGTTAGAGCAACTGTTCAGTCATCAGCTTCCCTCCTATTGCCGTTATTTGGCAAGAAATTACTAAAACAGAGAGTGAAAGTGTTTGGGACTCTTGTGAAAGGCCTCAGATTTGGACTGGATTTGTTAACGGTATTACAAAATGAAGGGCATATAATTGAGCTGTTATTAAAACTATTTAAAGTTCCACGTCTAAGTGGCAGTGATGGGCTTGATCAATATCTTAAGCAATTTGAAGCAGCTGTAGATAGTGACTTCCCAAATTACCAGAATCATGATGCAACCGATTCGGATGCTGAAATACTCTCGATTCTAGTCGAGCTATTTATGGAGAAAGCAACTGAGTGGACTGATGTCATACATGCCATCAACTGTATTGATGTGCTAAGATCTTTTGTAGTTACTGCAAGCTTCCCAAGTGGAGCTATGTCCAGACCTGTTCTTTTGCCTCAATCTCAAAATGCAACCTTGAATGGAGAAAATGGAAGTCCTACACTTAATATTAAAGGACTATGGCATCCATTTGCTCTTGGTGAAAATGGTGGACAGCCTGTGCCAAATGACGTAGTTCTAGGTGAAAATACTGATGGCTATCATCCACGCACTTTACTATTAACTGGACCAAATATGGGTGGGAAATCAACTCTTCTTCGTTCCACATGTCTGGCTGTTATACTTGCCCAGCTGGGCTGTTACGTGCCTTGTGAGATATGTGTTCTCTCACTTGTGGACATCATCTTTACACGGCTTGGTGCCACTGATCGTATCATGACTGGCGAGAGTACCTTCTTAGTGGAATGCACAGAAACGGCATCAGTTCTTCAACATGCCACTCCAGATTCTTTGGTTATTCTTGATGAATTGGGTCGTGGAACAAGCACGTTTGATGGATATGCCATTGCATATGCTGTATTTCGTCATCTTGTCGAAAAGATCAACTGCAGACTGTTATTCGCCACACACTATCACCCGCTCACGAGAGAATTTGCTTCTCATCCGCATGTTTCATTGCAACACATGGCATGTGCTTTTAGGTCGAAATCTAAATCCCCTTCAAAATGTGTCCAAGAGCTGGTATTTCTCTATCGCCTTACCTCAGGGGCATGCCCAGAGAGTTATGGATTGCAAGTAGCAATGATGGCTGGGATCCCTGAGAATGTGGTTGAAGCTGCTTCTAAGGCTGGCCAAGTAATGAAGAAATCTGTTGGAGAAAGCTTCAGGTCCAGTGAACAGAGATCGGAATTCTCAACTCTCCATGAGGAGTGGTTGAAGACACTGTTAACAGTTTCACAAACCGGTAATTATAACGACGAAGATTCATTGGACGTATTGTTTTGCTTGCAGCAGGAACTAAAGTGCTCATATAGATGGGCCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000217 GO:0000404 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006259 GO:0006281 GO:0006298 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008094 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030983 GO:0032135 GO:0032300 GO:0032991 GO:0033554 GO:0034641 GO:0042623 GO:0043170 GO:0043570 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990391

KEGG Pathways

Metabolic & Signaling

ko01524 ko03430 ko05200 ko05210 map01524 map03430 map05200 map05210

Pfam Domains

Protein Families

MutS_I MutS_II MutS_III MutS_V

Protein Analysis

1073

Amino Acids

118.16

Weight (kDa)

6.28

Isoelectric Point (pI)

40.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MutS_I	PF01624	242 - 353	2.6e-29	MutS domain I
MutS_II	PF05188	362 - 505	1e-11	MutS domain II
MutS_III	PF05192	528 - 745	1.3e-32	MutS domain III
MutS_V	PF00488	817 - 1009	3.7e-73	MutS domain V

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014491)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24495 AT3G24495
fragaria_vesca	FvH4_1g05130 FvH4_1g05130
malus_domestica	MD15G1187500.v1.1
prunus_persica	Prupe.7G228900_v2.0.a1
pyrus_communis	pycom15g16830
rosa_chinensis	RchiOBHm_Chr2g0090761
rosa_laevigata	RLG00000016141
rosa_multiflora	Rmu_ssc0000367.1_g000007
rosa_roxburghii	Rroxscaffold_2G00150840
rosa_rugosa	Rorug02G0010300
rosa_samantha	Rh2AG056300 Rh2BG055000 Rh2CG057400 Rh2DG055900
rosa_wichuraiana	Rw2G004620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1557, 3144
Acc36I	ACCTGC	1 cut(s)	1477
AccB1I	GGYRCC	4 cut(s)	181, 995, 1783, 2591
AccB7I	CCANNNNNTGG	2 cut(s)	314, 2911
AccBSI	CCGCTC	1 cut(s)	2812
AccI	GTMKAC	1 cut(s)	1134
AccII	CGCG	1 cut(s)	101
AccIII	TCCGGA	2 cut(s)	33, 139
AclWI	GGATC	8 cut(s)	13, 120, 133, 150, 656, 1709, 2980, 2993
AcoI	YGGCCR	3 cut(s)	79, 826, 3022
AcsI	RAATTY	6 cut(s)	205, 471, 503, 1417, 2822, 3080
AcuI	CTGAAG	3 cut(s)	728, 1389, 3040
AfaI	GTAC	6 cut(s)	334, 1304, 1449, 1480, 1639, 2623
AfeI	AGCGCT	1 cut(s)	1269
AfiI	CCNNNNNNNGG	8 cut(s)	33, 314, 494, 1475, 1573, 1889, 2468, 2911
AflII	CTTAAG	1 cut(s)	2063
AflIII	ACRYGT	1 cut(s)	2498
AgeI	ACCGGT	1 cut(s)	3134
AhdI	GACNNNNNGTC	1 cut(s)	447
AjiI	CACGTC	2 cut(s)	188, 2030
AjnI	CCWGG	2 cut(s)	655, 1499
AjuI	GAANNNNNNNTTGG	2 cut(s)	301, 333
AloI	GAACNNNNNNTCC	2 cut(s)	151, 183
Alw21I	GWGCWC	1 cut(s)	3200
AlwI	GGATC	8 cut(s)	13, 120, 133, 150, 656, 1709, 2980, 2993
AlwNI	CAGNNNCTG	3 cut(s)	196, 341, 1588
Aor13HI	TCCGGA	2 cut(s)	33, 139
Aor51HI	AGCGCT	1 cut(s)	1269
AoxI	GGCC	7 cut(s)	79, 653, 826, 1067, 1942, 3022, 3210
ApeKI	GCWGC	7 cut(s)	4, 961, 1208, 1339, 2078, 3008, 3181
ApoI	RAATTY	6 cut(s)	205, 471, 503, 1417, 2822, 3080
AseI	ATTAAT	2 cut(s)	378, 1734
AsiGI	ACCGGT	1 cut(s)	3134
AspLEI	GCGC	2 cut(s)	9, 1270
AspS9I	GGNCC	7 cut(s)	425, 1067, 1433, 1628, 2459, 3060, 3210
AsuC2I	CCSGG	1 cut(s)	438
AsuHPI	GGTGA	4 cut(s)	671, 2384, 2426, 2798
AsuII	TTCGAA	1 cut(s)	1415
AsuNHI	GCTAGC	1 cut(s)	599
AvaII	GGWCC	5 cut(s)	425, 1433, 1628, 2459, 3060
AxyI	CCTNAGG	1 cut(s)	2935
BalI	TGGCCA	2 cut(s)	828, 3024
BamHI	GGATCC	2 cut(s)	125, 2985
BanI	GGYRCC	4 cut(s)	181, 995, 1783, 2591
BanII	GRGCYC	1 cut(s)	120
BauI	CACGAG	1 cut(s)	2815
BbsI	GAAGAC	2 cut(s)	1404, 3116
Bbv12I	GWGCWC	1 cut(s)	3200
BbvI	GCAGC	7 cut(s)	16, 973, 1195, 1351, 2090, 2995, 3193
BceAI	ACGGC	5 cut(s)	66, 94, 1867, 2600, 2664
BcgI	CGANNNNNNTGC	4 cut(s)	2109, 2114, 2143, 2148
BciT130I	CCWGG	2 cut(s)	657, 1501
BclI	TGATCA	2 cut(s)	976, 2053
BcnI	CCSGG	1 cut(s)	438
BfaI	CTAG	7 cut(s)	240, 452, 528, 600, 812, 2153, 2411
BfmI	CTRYAG	2 cut(s)	2082, 2779
BfoI	RGCGCY	2 cut(s)	10, 1271
BfrI	CTTAAG	1 cut(s)	2063
BfuAI	ACCTGC	1 cut(s)	1477
BglII	AGATCT	2 cut(s)	1759, 2228
BisI	GCNGC	9 cut(s)	5, 102, 962, 1209, 1340, 1685, 2079, 3009, 3182
BlsI	GCNGC	9 cut(s)	6, 103, 963, 1210, 1341, 1686, 2080, 3010, 3183
BmcAI	AGTACT	1 cut(s)	1304
Bme1390I	CCNGG	3 cut(s)	438, 657, 1501
Bme18I	GGWCC	5 cut(s)	425, 1433, 1628, 2459, 3060
BmeRI	GACNNNNNGTC	1 cut(s)	447
BmgBI	CACGTC	2 cut(s)	188, 2030
BmgT120I	GGNCC	7 cut(s)	425, 1067, 1433, 1628, 2459, 3060, 3210
BmrFI	CCNGG	3 cut(s)	438, 657, 1501
BmrI	ACTGGG	1 cut(s)	748
BmtI	GCTAGC	1 cut(s)	603
BmuI	ACTGGG	1 cut(s)	748
BoxI	GACNNNNGTC	1 cut(s)	2192
BpiI	GAAGAC	2 cut(s)	1404, 3116
BplI	GAGNNNNNCTC	2 cut(s)	1091, 1123
BpmI	CTGGAG	2 cut(s)	1317, 2658
Bpu14I	TTCGAA	1 cut(s)	1415
BpuEI	CTTGAG	3 cut(s)	569, 877, 1619
BpuMI	CCSGG	1 cut(s)	438
Bsa29I	ATCGAT	2 cut(s)	17, 360
BsaAI	YACGTR	1 cut(s)	2536
BsaBI	GATNNNNATC	4 cut(s)	398, 642, 2058, 2430
BsaJI	CCNNGG	4 cut(s)	82, 437, 577, 934
BsaWI	WCCGGW	4 cut(s)	33, 139, 297, 3134
BsaXI	ACNNNNNCTCC	2 cut(s)	151, 181
Bsc4I	CCNNNNNNNGG	8 cut(s)	33, 314, 494, 1475, 1573, 1889, 2468, 2911
Bse118I	RCCGGY	2 cut(s)	199, 3134
Bse1I	ACTGG	9 cut(s)	366, 743, 1334, 1343, 1770, 1962, 2461, 2617, 3063
Bse21I	CCTNAGG	1 cut(s)	2935
Bse3DI	GCAATG	6 cut(s)	417, 1126, 1534, 2736, 2846, 2979
Bse8I	GATNNNNATC	4 cut(s)	398, 642, 2058, 2430
BseAI	TCCGGA	2 cut(s)	33, 139
BseBI	CCWGG	2 cut(s)	657, 1501
BseCI	ATCGAT	2 cut(s)	17, 360
BseDI	CCNNGG	4 cut(s)	82, 437, 577, 934
BseJI	GATNNNNATC	4 cut(s)	398, 642, 2058, 2430
BseLI	CCNNNNNNNGG	8 cut(s)	33, 314, 494, 1475, 1573, 1889, 2468, 2911
BseMI	GCAATG	6 cut(s)	417, 1126, 1534, 2736, 2846, 2979
BseMII	CTCAG	6 cut(s)	165, 1811, 1960, 2172, 2949, 2982
BseNI	ACTGG	9 cut(s)	366, 743, 1334, 1343, 1770, 1962, 2461, 2617, 3063
BseRI	GAGGAG	3 cut(s)	494, 566, 3113
BseX3I	CGGCCG	1 cut(s)	79
BseXI	GCAGC	7 cut(s)	16, 973, 1195, 1351, 2090, 2995, 3193
BseYI	CCCAGC	3 cut(s)	2520, 2524, 2981
BsgI	GTGCAG	1 cut(s)	1358
Bsh1236I	CGCG	1 cut(s)	101
Bsh1285I	CGRYCG	1 cut(s)	82
BshFI	GGCC	7 cut(s)	81, 655, 828, 1069, 1944, 3024, 3212
BshNI	GGYRCC	4 cut(s)	181, 995, 1783, 2591
BshTI	ACCGGT	1 cut(s)	3134
BshVI	ATCGAT	2 cut(s)	17, 360
BsiEI	CGRYCG	1 cut(s)	82
BsiHKAI	GWGCWC	1 cut(s)	3200
BsiSI	CCGG	7 cut(s)	34, 69, 140, 200, 298, 437, 3135
BslFI	GGGAC	2 cut(s)	454, 1942
BslI	CCNNNNNNNGG	8 cut(s)	33, 314, 494, 1475, 1573, 1889, 2468, 2911
BsmFI	GGGAC	2 cut(s)	454, 1942
BsmI	GAATGC	1 cut(s)	2642
BsnI	GGCC	7 cut(s)	81, 655, 828, 1069, 1944, 3024, 3212
Bsp119I	TTCGAA	1 cut(s)	1415
Bsp1286I	GDGCHC	2 cut(s)	120, 3200
Bsp13I	TCCGGA	2 cut(s)	33, 139
Bsp1407I	TGTACA	1 cut(s)	332
Bsp19I	CCATGG	1 cut(s)	577
BspANI	GGCC	7 cut(s)	81, 655, 828, 1069, 1944, 3024, 3212
BspCNI	CTCAG	6 cut(s)	166, 1810, 1959, 2173, 2948, 2983
BspDI	ATCGAT	2 cut(s)	17, 360
BspEI	TCCGGA	2 cut(s)	33, 139
BspFNI	CGCG	1 cut(s)	101
BspHI	TCATGA	2 cut(s)	2113, 2607
BspMAI	CTGCAG	1 cut(s)	2783
BspMI	ACCTGC	1 cut(s)	1477
BspOI	GCTAGC	1 cut(s)	603
BspPI	GGATC	8 cut(s)	13, 120, 133, 150, 656, 1709, 2980, 2993
BspT104I	TTCGAA	1 cut(s)	1415
BspT107I	GGYRCC	4 cut(s)	181, 995, 1783, 2591
BspTI	CTTAAG	1 cut(s)	2063
BsrBI	CCGCTC	1 cut(s)	2812
BsrDI	GCAATG	6 cut(s)	417, 1126, 1534, 2736, 2846, 2979
BsrFI	RCCGGY	2 cut(s)	199, 3134
BsrGI	TGTACA	1 cut(s)	332
BsrI	ACTGG	9 cut(s)	366, 743, 1334, 1343, 1770, 1962, 2461, 2617, 3063
BssAI	RCCGGY	2 cut(s)	199, 3134
BssECI	CCNNGG	4 cut(s)	82, 437, 577, 934
BssNAI	GTATAC	1 cut(s)	1135
BssSI	CACGAG	1 cut(s)	2815
BssT1I	CCWWGG	1 cut(s)	577
Bst1107I	GTATAC	1 cut(s)	1135
Bst2BI	CACGAG	1 cut(s)	2815
Bst2UI	CCWGG	2 cut(s)	657, 1501
Bst4CI	ACNGT	9 cut(s)	337, 562, 1664, 1855, 1972, 2213, 2787, 3117, 3124
Bst6I	CTCTTC	2 cut(s)	232, 2490
BstAFI	CTTAAG	1 cut(s)	2063
BstAUI	TGTACA	1 cut(s)	332
BstBAI	YACGTR	1 cut(s)	2536
BstBI	TTCGAA	1 cut(s)	1415
BstC8I	GCNNGC	8 cut(s)	201, 214, 601, 1811, 2248, 2944, 3022, 3179
BstDSI	CCRYGG	3 cut(s)	82, 577, 934
BstFNI	CGCG	1 cut(s)	101
BstH2I	RGCGCY	2 cut(s)	10, 1271
BstHHI	GCGC	2 cut(s)	9, 1270
BstMCI	CGRYCG	1 cut(s)	82
BstMWI	GCNNNNNNNGC	9 cut(s)	209, 466, 995, 1205, 1217, 1237, 1289, 2978, 3017
BstNI	CCWGG	2 cut(s)	657, 1501
BstNSI	RCATGY	6 cut(s)	2204, 2502, 2669, 2843, 2865, 2946
BstPAI	GACNNNNGTC	1 cut(s)	2192
BstSCI	CCNGG	3 cut(s)	436, 655, 1499
BstSFI	CTRYAG	2 cut(s)	2082, 2779
BstUI	CGCG	1 cut(s)	101
BstV1I	GCAGC	7 cut(s)	16, 973, 1195, 1351, 2090, 2995, 3193
BstV2I	GAAGAC	2 cut(s)	1404, 3116
BstX2I	RGATCY	6 cut(s)	125, 142, 1701, 1759, 2228, 2985
BstXI	CCANNNNNNTGG	1 cut(s)	2503
BstYI	RGATCY	6 cut(s)	125, 142, 1701, 1759, 2228, 2985
BstZ17I	GTATAC	1 cut(s)	1135
BstZI	CGGCCG	1 cut(s)	79
Bsu15I	ATCGAT	2 cut(s)	17, 360
Bsu36I	CCTNAGG	1 cut(s)	2935
BsuRI	GGCC	7 cut(s)	81, 655, 828, 1069, 1944, 3024, 3212
BsuTUI	ATCGAT	2 cut(s)	17, 360
BtgI	CCRYGG	3 cut(s)	82, 577, 934
BtrI	CACGTC	2 cut(s)	188, 2030
BtsI	GCAGTG	2 cut(s)	1319, 2047
BtsIMutI	CAGTG	8 cut(s)	1195, 1292, 1319, 1341, 2047, 2595, 3070, 3113
BveI	ACCTGC	1 cut(s)	1477
Cac8I	GCNNGC	8 cut(s)	201, 214, 601, 1811, 2248, 2944, 3022, 3179
CaiI	CAGNNNCTG	3 cut(s)	196, 341, 1588
CciI	TCATGA	2 cut(s)	2113, 2607
CfoI	GCGC	2 cut(s)	9, 1270
Cfr10I	RCCGGY	2 cut(s)	199, 3134
Cfr13I	GGNCC	7 cut(s)	425, 1067, 1433, 1628, 2459, 3060, 3210
ClaI	ATCGAT	2 cut(s)	17, 360
CseI	GACGC	1 cut(s)	1594
Csp6I	GTAC	6 cut(s)	333, 1303, 1448, 1479, 1638, 2622
CspAI	ACCGGT	1 cut(s)	3134
CviQI	GTAC	6 cut(s)	333, 1303, 1448, 1479, 1638, 2622
DraI	TTTAAA	3 cut(s)	781, 1167, 2020
DriI	GACNNNNNGTC	1 cut(s)	447
EaeI	YGGCCR	3 cut(s)	79, 826, 3022
EagI	CGGCCG	1 cut(s)	79
Eam1104I	CTCTTC	2 cut(s)	232, 2490
Eam1105I	GACNNNNNGTC	1 cut(s)	447
EarI	CTCTTC	2 cut(s)	232, 2490
EciI	GGCGGA	1 cut(s)	159
EclXI	CGGCCG	1 cut(s)	79
Eco130I	CCWWGG	1 cut(s)	577
Eco147I	AGGCCT	2 cut(s)	655, 1944
Eco24I	GRGCYC	1 cut(s)	120
Eco32I	GATATC	1 cut(s)	358
Eco47I	GGWCC	5 cut(s)	425, 1433, 1628, 2459, 3060
Eco47III	AGCGCT	1 cut(s)	1269
Eco52I	CGGCCG	1 cut(s)	79
Eco57I	CTGAAG	3 cut(s)	728, 1389, 3040
Eco81I	CCTNAGG	1 cut(s)	2935
EcoRI	GAATTC	3 cut(s)	205, 471, 3080
EcoRII	CCWGG	2 cut(s)	655, 1499
EcoRV	GATATC	1 cut(s)	358
EcoT14I	CCWWGG	1 cut(s)	577
EcoT22I	ATGCAT	3 cut(s)	252, 399, 1651
EcoT38I	GRGCYC	1 cut(s)	120
ErhI	CCWWGG	1 cut(s)	577
FaqI	GGGAC	2 cut(s)	454, 1942
FauI	CCCGC	1 cut(s)	2817
FauNDI	CATATG	2 cut(s)	462, 2743
FbaI	TGATCA	2 cut(s)	976, 2053
FblI	GTMKAC	1 cut(s)	1134
Fnu4HI	GCNGC	9 cut(s)	5, 102, 962, 1209, 1340, 1685, 2079, 3009, 3182
FriOI	GRGCYC	1 cut(s)	120
Fsp4HI	GCNGC	9 cut(s)	5, 102, 962, 1209, 1340, 1685, 2079, 3009, 3182
FspBI	CTAG	7 cut(s)	240, 452, 528, 600, 812, 2153, 2411
GlaI	GCGC	2 cut(s)	8, 1269
GluI	GCNGC	9 cut(s)	5, 102, 962, 1209, 1340, 1685, 2079, 3009, 3182
GsaI	CCCAGC	3 cut(s)	2524, 2528, 2985
GsuI	CTGGAG	2 cut(s)	1317, 2658
HaeII	RGCGCY	2 cut(s)	10, 1271
HaeIII	GGCC	7 cut(s)	81, 655, 828, 1069, 1944, 3024, 3212
HapII	CCGG	7 cut(s)	34, 69, 140, 200, 298, 437, 3135
HgaI	GACGC	1 cut(s)	1594
HhaI	GCGC	2 cut(s)	9, 1270
Hin6I	GCGC	2 cut(s)	7, 1268
HinP1I	GCGC	2 cut(s)	7, 1268
HincII	GTYRAC	2 cut(s)	1968, 3120
HindII	GTYRAC	2 cut(s)	1968, 3120
HindIII	AAGCTT	3 cut(s)	1811, 2248, 3052
HpaI	GTTAAC	2 cut(s)	1968, 3120
HpaII	CCGG	7 cut(s)	34, 69, 140, 200, 298, 437, 3135
HphI	GGTGA	4 cut(s)	671, 2384, 2426, 2798
Hpy99I	CGWCG	2 cut(s)	125, 3152
HpyAV	CCTTC	8 cut(s)	37, 100, 172, 256, 645, 1979, 2635, 2901
HpyCH4III	ACNGT	9 cut(s)	337, 562, 1664, 1855, 1972, 2213, 2787, 3117, 3124
HpyCH4IV	ACGT	8 cut(s)	187, 448, 1518, 2029, 2403, 2535, 2720, 3165
HpyF10VI	GCNNNNNNNGC	9 cut(s)	209, 466, 995, 1205, 1217, 1237, 1289, 2978, 3017
HpySE526I	ACGT	8 cut(s)	187, 448, 1518, 2029, 2403, 2535, 2720, 3165
HspAI	GCGC	2 cut(s)	7, 1268
Kpn2I	TCCGGA	2 cut(s)	33, 139
KroI	GCCGGC	1 cut(s)	199
KroNI	GCCGGC	1 cut(s)	201
Ksp22I	TGATCA	2 cut(s)	976, 2053
KspAI	GTTAAC	2 cut(s)	1968, 3120
LmnI	GCTCC	5 cut(s)	109, 115, 553, 835, 2261
Lsp1109I	GCAGC	7 cut(s)	16, 973, 1195, 1351, 2090, 2995, 3193
MaeI	CTAG	7 cut(s)	240, 452, 528, 600, 812, 2153, 2411
MaeII	ACGT	8 cut(s)	187, 448, 1518, 2029, 2403, 2535, 2720, 3165
MaeIII	GTNAC	5 cut(s)	1033, 1666, 2090, 2239, 2531
MbiI	CCGCTC	1 cut(s)	2812
MfeI	CAATTG	1 cut(s)	1325
MflI	RGATCY	6 cut(s)	125, 142, 1701, 1759, 2228, 2985
MhlI	GDGCHC	2 cut(s)	120, 3200
MlsI	TGGCCA	2 cut(s)	828, 3024
MluNI	TGGCCA	2 cut(s)	828, 3024
MlyI	GAGTC	3 cut(s)	218, 1324, 1924
MmeI	TCCRAC	3 cut(s)	103, 928, 3025
Mox20I	TGGCCA	2 cut(s)	828, 3024
Mph1103I	ATGCAT	3 cut(s)	252, 399, 1651
MroI	TCCGGA	2 cut(s)	33, 139
MroNI	GCCGGC	1 cut(s)	199
MscI	TGGCCA	2 cut(s)	828, 3024
MslI	CAYNNNNRTG	3 cut(s)	300, 2838, 3205
Msp20I	TGGCCA	2 cut(s)	828, 3024
MspA1I	CMGCKG	3 cut(s)	964, 2081, 2524
MspCI	CTTAAG	1 cut(s)	2063
MspI	CCGG	7 cut(s)	34, 69, 140, 200, 298, 437, 3135
MspR9I	CCNGG	3 cut(s)	438, 657, 1501
MunI	CAATTG	1 cut(s)	1325
Mva1269I	GAATGC	1 cut(s)	2642
MvaI	CCWGG	2 cut(s)	657, 1501
MvnI	CGCG	1 cut(s)	101
MwoI	GCNNNNNNNGC	9 cut(s)	209, 466, 995, 1205, 1217, 1237, 1289, 2978, 3017
NaeI	GCCGGC	1 cut(s)	201
NciI	CCSGG	1 cut(s)	438
NcoI	CCATGG	1 cut(s)	577
NdeI	CATATG	2 cut(s)	462, 2743
NgoMIV	GCCGGC	1 cut(s)	199
NheI	GCTAGC	1 cut(s)	599
NmuCI	GTSAC	3 cut(s)	1033, 1666, 2090
NsiI	ATGCAT	3 cut(s)	252, 399, 1651
NspI	RCATGY	6 cut(s)	2204, 2502, 2669, 2843, 2865, 2946
NspV	TTCGAA	1 cut(s)	1415
PaeI	GCATGC	1 cut(s)	2946
PagI	TCATGA	2 cut(s)	2113, 2607
PceI	AGGCCT	2 cut(s)	655, 1944
PciI	ACATGT	1 cut(s)	2498
PctI	GAATGC	1 cut(s)	2642
PdiI	GCCGGC	1 cut(s)	201
PflMI	CCANNNNNTGG	2 cut(s)	314, 2911
PinAI	ACCGGT	1 cut(s)	3134
PkrI	GCNGC	9 cut(s)	6, 103, 963, 1210, 1341, 1686, 2080, 3010, 3183
PleI	GAGTC	3 cut(s)	218, 1324, 1924
PpsI	GAGTC	3 cut(s)	218, 1324, 1924
Ppu21I	YACGTR	1 cut(s)	2536
PscI	ACATGT	1 cut(s)	2498
PshAI	GACNNNNGTC	1 cut(s)	2192
PshBI	ATTAAT	2 cut(s)	378, 1734
PsiI	TTATAA	2 cut(s)	1557, 3144
Psp6I	CCWGG	2 cut(s)	655, 1499
PspFI	CCCAGC	3 cut(s)	2520, 2524, 2981
PspGI	CCWGG	2 cut(s)	655, 1499
PspPI	GGNCC	7 cut(s)	425, 1067, 1433, 1628, 2459, 3060, 3210
PstI	CTGCAG	1 cut(s)	2783
PstNI	CAGNNNCTG	3 cut(s)	196, 341, 1588
PsuI	RGATCY	6 cut(s)	125, 142, 1701, 1759, 2228, 2985
PvuII	CAGCTG	3 cut(s)	964, 2081, 2524
RsaI	GTAC	6 cut(s)	334, 1304, 1449, 1480, 1639, 2623
RsaNI	GTAC	6 cut(s)	333, 1303, 1448, 1479, 1638, 2622
RseI	CAYNNNNRTG	3 cut(s)	300, 2838, 3205
SatI	GCNGC	9 cut(s)	5, 102, 962, 1209, 1340, 1685, 2079, 3009, 3182
Sau96I	GGNCC	7 cut(s)	425, 1067, 1433, 1628, 2459, 3060, 3210
ScaI	AGTACT	1 cut(s)	1304
SchI	GAGTC	3 cut(s)	218, 1324, 1924
ScrFI	CCNGG	3 cut(s)	438, 657, 1501
SduI	GDGCHC	2 cut(s)	120, 3200
SfcI	CTRYAG	2 cut(s)	2082, 2779
SfuI	TTCGAA	1 cut(s)	1415
SinI	GGWCC	5 cut(s)	425, 1433, 1628, 2459, 3060
SmiMI	CAYNNNNRTG	3 cut(s)	300, 2838, 3205
SmlI	CTYRAG	4 cut(s)	548, 856, 1598, 2063
SmoI	CTYRAG	4 cut(s)	548, 856, 1598, 2063
SphI	GCATGC	1 cut(s)	2946
SseBI	AGGCCT	2 cut(s)	655, 1944
SspI	AATATT	2 cut(s)	1063, 2341
SspMI	CTAG	7 cut(s)	240, 452, 528, 600, 812, 2153, 2411
StuI	AGGCCT	2 cut(s)	655, 1944
StyD4I	CCNGG	3 cut(s)	436, 655, 1499
StyI	CCWWGG	1 cut(s)	577
TaaI	ACNGT	9 cut(s)	337, 562, 1664, 1855, 1972, 2213, 2787, 3117, 3124
TaiI	ACGT	8 cut(s)	190, 451, 1521, 2032, 2406, 2538, 2723, 3168
TatI	WGTACW	2 cut(s)	332, 1302
TauI	GCSGC	2 cut(s)	104, 1687
TscAI	CASTG	8 cut(s)	1195, 1299, 1326, 1341, 2047, 2602, 3070, 3120
TseFI	GTSAC	3 cut(s)	1033, 1666, 2090
TseI	GCWGC	7 cut(s)	4, 961, 1208, 1339, 2078, 3008, 3181
Tsp45I	GTSAC	3 cut(s)	1033, 1666, 2090
TspGWI	ACGGA	2 cut(s)	923, 967
TspRI	CASTG	8 cut(s)	1195, 1299, 1326, 1341, 2047, 2602, 3070, 3120
Van91I	CCANNNNNTGG	2 cut(s)	314, 2911
Vha464I	CTTAAG	1 cut(s)	2063
VpaK11BI	GGWCC	5 cut(s)	425, 1433, 1628, 2459, 3060
VspI	ATTAAT	2 cut(s)	378, 1734
XapI	RAATTY	6 cut(s)	205, 471, 503, 1417, 2822, 3080
XceI	RCATGY	6 cut(s)	2204, 2502, 2669, 2843, 2865, 2946
XmiI	GTMKAC	1 cut(s)	1134
XspI	CTAG	7 cut(s)	240, 452, 528, 600, 812, 2153, 2411
ZrmI	AGTACT	1 cut(s)	1304
Zsp2I	ATGCAT	3 cut(s)	252, 399, 1651

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.