Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0014491

Physical Location & Seq

Genomic Coordinates Forward (+)

4069637 .. 4076558

Gene Structure 6922 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG055900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2760 bp

ATGCAGCGCCAGAAATCGATCCTCTCCTTCTTCCGGAAACCCTCGCCGGACAAAAGCACCTCCACCGCAGGAGACGACGGCCGTGGAGTTAATCAGTTCGCGGAGAAGGAGCGGGAGCAGAAGCCCGTCGGATCCAATCAGCCTCCGGATCTCGAAGTTCGAGGAACCGACACTCCGCCTGAGAAGGTGCCACGTCAGATTCTGCCGGCGAATTCTGCGAGCCTGAAAGACTCTTCGCCTTTCTCTAGTATTATGCATAAGTTTATGAAGGTCGATGACACACCTAAGGCTTCTCAGAGCCACCGGAATGAACCAAATGGTGGTTCCTCTAATGATTGTACAGTATCTGGTAAACTCAATGATATCAATTCCAGTAAGAAAATTAATCGAGGTGCTGTGATGCATATCGAAAGTGATGAGGACATTGCTGGACCAGAAACACCAGGGACACAACGTCTAGTTCCGCGTATGAAGCGAATTCAAGAGGAGATTCCTAAGTTTGGGGATAAATTTGGGAGTTCTTTGGCAGATTCTAGCAAAAGATTGAAAGTTCTTGAGGAGCCAACAGTCTTCGACAAGAGCCATGGGGAAGCATCTGATATGGCTAGCAAGTTTGAGTGGCTTGACCCATCCCGAATCAGAGATGCAAATCGCAGAAGGCCTGGTGATCCTCTTTATGACAAAACATCACTTTATATACCGCCTGATGCTCTGAGGAAAATGTCGGCATCACAAAGACAATACTGGGATGTCAAGTGTCAGTATATGGATGTTGTGATTTTCTTTAAAGTGGGAAAGTTTTATGAGCTTTATGAACTGGATGCTGAAATTGGCCACAAGGAGCTTGATTGGAAAATGACCTTGAGTGGTGTTGGGAAATGTCGTCAGGTTGGCATCTCTGAAAGTGGGATTGACGATGCTGTTCAAAAGTTGGTTGTCCGTGGGTATAAAGTTGGACGGATAGAGCAGCTGGAAACTTCTGATCAAGTAAAAGCCAGAGGTGCCAAAGCTGTAATTCCAAGAAAATTGGTTCAGGTAGTCACACCATCAACCACAACTGATGGAAATATTGGGCCTGATGCTGTTCATCTTCTTGCAATAAAAGAGGGGAACTCTGGGGTCGACAATGGTTCAGTTGTATACGGATTTGCTTTTGTTGATTGTTCTGCTTTAAAGTTCTGGATTGGTGCTATCAGTGATGATGCTTCGTGTGCTGCTTTGGGGGCTTTATTGATGCAAGTGTCACCCAAAGAAGTTGTATATGAAAACAGAGGGCTCTCAAAAGAAGCTCAAAAAGCACTGAAAAAGTACTCAACAGGTTCTGCACTGCAATTGACTCCAGTGCAGCCAGTCAATGATTTTATGGATGCTTCTGAAGTTAGTAAATTGATTCAGTTGAAAGGATACTTCAAAGGGTCTTCGAATTCATGGAATCATGGACCTGATGGTGTGGTACACCATGATATAACTTTACCTGCTCTGGGTACACTTATTGACCACCTATCCAGGTTGATGTTAGATGATGTTTTACGCAATGGAGATATATTACCTTACCAAGTTTATAAGGGTTGCCTCAAAATGGATGGACAGACGCTGATAAATCTTGAGATTTTCAGTAACACTGCTGATGGTGGTCCATCAGGTACATTGTATGCATATCTCGATAACTGTGTGACTGCATCTGGTAAGCGGCTTTTGAGGAAGTGGATCTGCCATCCCTTGAAGTCTGTTGAAGCTATTAATAACAGGCTTAATTTGGTTGAAGATCTACTGGCACATCCAGAAATGGTGCCACTTATTGCTCAGTATCTTCGCAAGCTGCCAGACTTAGAGAGGTTGTTTGGACGGGTTAGAGCAACTGTTCAGTCGTCAGCTTCCCTCCTATTGCCATTATTTGGCAAGAAATTACTAAAACAGAGAGTGAAAGTGTTCGGGACTCTTGTGAAAGGCCTCAGATTTGGACTGGATTTGTTAACGGTATTACAAAATGAAGGGCATATAATTGAGCTGTTATTAAAACTATTTAAAGTTCCACGTCTAAGTGGCAGTGATGGGCTTGATCAATTTCTTAAACAATTTGAAGCAGCTGTAGATAGTGACTTCCCAAATTACCAGAATCATGATGCAACCGATTCAGATGCTGAAATACTCTCGATTCTAGTCGAGCTATTTATGGAGAAAGCAACTGAGTGGACTGATGTCATACATGCCATCAACTGTATTGATGTGCTAAGATCTTTTGTAGTTACCGCAAGCTTCCCAAGTGGAGCTATGTCCAGACCTGTTCTTTTGCCTCAATCTCAAAATACAACCTTGAATGGAGAAAATGGAAGTCCTACACTTAATATTAAAGGACTATGGCATCCATTTGCTCTTGGTGAAAATGGTGGACAGCCTGTGCCAAATGACGTAGTTCTAGGTGAAAATACTGATGGCTATCATCCACGCACTTTACTATTAACTGGACCAAATATGGGTGGGAAATCAACTCTTCTTCGTTCCACATGTCTGGCTGTTATACTTGCCCAGCTGGGCTGTTACGTGCCTTGTGAGATATGTGTTCTCTCACTTGTGGACATCATCTTTACACGGCTTGGTGCCACTGATCGTATCATGACTGGCGAGAGTACCTTCTTAGTGGAATGCACGGAAACGGCATCAGTTCTTCAACATGCCACTCCAGATTCTTTGGTTATTCTTGATGAATTGGGTCGTGGAACAAGCACGTTTGATGGATATGCCATTGCATATTGCTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000217 GO:0000404 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006259 GO:0006281 GO:0006298 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008094 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030983 GO:0032135 GO:0032300 GO:0032991 GO:0033554 GO:0034641 GO:0042623 GO:0043170 GO:0043570 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990391

KEGG Pathways

Metabolic & Signaling

ko01524 ko03430 ko05200 ko05210 map01524 map03430 map05200 map05210

Pfam Domains

Protein Families

MutS_I MutS_II MutS_III MutS_V

Protein Analysis

919

Amino Acids

100.9

Weight (kDa)

6.27

Isoelectric Point (pI)

36.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MutS_I	PF01624	244 - 355	2.1e-29	MutS domain I
MutS_II	PF05188	364 - 509	1.8e-11	MutS domain II
MutS_III	PF05192	530 - 747	9.4e-33	MutS domain III
MutS_V	PF00488	819 - 918	1.7e-45	MutS domain V

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014491)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24495 AT3G24495
fragaria_vesca	FvH4_1g05130 FvH4_1g05130
malus_domestica	MD15G1187500.v1.1
prunus_persica	Prupe.7G228900_v2.0.a1
pyrus_communis	pycom15g16830
rosa_chinensis	RchiOBHm_Chr2g0090761
rosa_laevigata	RLG00000016141
rosa_multiflora	Rmu_ssc0000367.1_g000007
rosa_roxburghii	Rroxscaffold_2G00150840
rosa_rugosa	Rorug02G0010300
rosa_samantha	Rh2AG056300 Rh2BG055000 Rh2CG057400 Rh2DG055900
rosa_wichuraiana	Rw2G004620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1563
Acc36I	ACCTGC	1 cut(s)	1483
AccB1I	GGYRCC	4 cut(s)	187, 1001, 1789, 2597
AccB7I	CCANNNNNTGG	2 cut(s)	320, 1895
AccBSI	CCGCTC	1 cut(s)	112
AccI	GTMKAC	2 cut(s)	1122, 1140
AccII	CGCG	2 cut(s)	101, 466
AccIII	TCCGGA	2 cut(s)	33, 145
AciI	CCGC	8 cut(s)	66, 101, 112, 176, 464, 701, 1690, 2250
AclWI	GGATC	6 cut(s)	13, 126, 139, 156, 662, 1715
AcoI	YGGCCR	2 cut(s)	79, 832
AcsI	RAATTY	4 cut(s)	211, 477, 509, 1423
AcuI	CTGAAG	1 cut(s)	1395
AfaI	GTAC	6 cut(s)	340, 1310, 1455, 1486, 1645, 2629
AfiI	CCNNNNNNNGG	7 cut(s)	33, 320, 500, 1481, 1579, 1895, 2474
AflIII	ACRYGT	1 cut(s)	2504
AhdI	GACNNNNNGTC	1 cut(s)	453
AjiI	CACGTC	2 cut(s)	194, 2036
AjnI	CCWGG	3 cut(s)	442, 661, 1505
AjuI	GAANNNNNNNTTGG	2 cut(s)	307, 339
AloI	GAACNNNNNNTCC	2 cut(s)	157, 189
Alw26I	GTCTC	1 cut(s)	66
AlwI	GGATC	6 cut(s)	13, 126, 139, 156, 662, 1715
AlwNI	CAGNNNCTG	5 cut(s)	202, 347, 1322, 1594, 2141
Aor13HI	TCCGGA	2 cut(s)	33, 145
AoxI	GGCC	5 cut(s)	79, 659, 832, 1073, 1948
ApeKI	GCWGC	6 cut(s)	4, 967, 1214, 1345, 1819, 2084
ApoI	RAATTY	4 cut(s)	211, 477, 509, 1423
AseI	ATTAAT	2 cut(s)	384, 1740
Asp700I	GAANNNNTTC	1 cut(s)	1928
AspLEI	GCGC	1 cut(s)	9
AspS9I	GGNCC	5 cut(s)	431, 1073, 1439, 1634, 2465
AsuHPI	GGTGA	4 cut(s)	677, 1236, 2390, 2432
AsuII	TTCGAA	1 cut(s)	1421
AsuNHI	GCTAGC	1 cut(s)	605
AvaII	GGWCC	4 cut(s)	431, 1439, 1634, 2465
AxyI	CCTNAGG	1 cut(s)	285
BalI	TGGCCA	1 cut(s)	834
BamHI	GGATCC	1 cut(s)	131
BanI	GGYRCC	4 cut(s)	187, 1001, 1789, 2597
BanII	GRGCYC	1 cut(s)	1278
BbsI	GAAGAC	2 cut(s)	562, 1410
BbvI	GCAGC	6 cut(s)	16, 979, 1201, 1357, 1806, 2096
BceAI	ACGGC	4 cut(s)	66, 94, 2606, 2670
BcgI	CGANNNNNNTGC	2 cut(s)	2120, 2154
BciT130I	CCWGG	3 cut(s)	444, 663, 1507
BciVI	GTATCC	1 cut(s)	1397
BclI	TGATCA	2 cut(s)	982, 2059
BcoDI	GTCTC	1 cut(s)	66
BfaI	CTAG	6 cut(s)	246, 458, 534, 606, 2159, 2417
BfmI	CTRYAG	1 cut(s)	2088
BfoI	RGCGCY	1 cut(s)	10
BfuAI	ACCTGC	1 cut(s)	1483
BfuI	GTATCC	1 cut(s)	1397
BglII	AGATCT	2 cut(s)	1765, 2234
BisI	GCNGC	7 cut(s)	5, 968, 1215, 1346, 1691, 1820, 2085
BlsI	GCNGC	7 cut(s)	6, 969, 1216, 1347, 1692, 1821, 2086
BmcAI	AGTACT	1 cut(s)	1310
Bme1390I	CCNGG	3 cut(s)	444, 663, 1507
Bme18I	GGWCC	4 cut(s)	431, 1439, 1634, 2465
BmeRI	GACNNNNNGTC	1 cut(s)	453
BmgBI	CACGTC	2 cut(s)	194, 2036
BmgT120I	GGNCC	5 cut(s)	431, 1073, 1439, 1634, 2465
BmiI	GGNNCC	8 cut(s)	133, 166, 189, 325, 561, 1003, 1791, 2599
BmrFI	CCNGG	3 cut(s)	444, 663, 1507
BmrI	ACTGGG	1 cut(s)	754
BmtI	GCTAGC	1 cut(s)	609
BmuI	ACTGGG	1 cut(s)	754
BoxI	GACNNNNGTC	1 cut(s)	2198
BpiI	GAAGAC	2 cut(s)	562, 1410
BplI	GAGNNNNNCTC	2 cut(s)	1097, 1129
BpmI	CTGGAG	2 cut(s)	1323, 2664
Bpu14I	TTCGAA	1 cut(s)	1421
BpuEI	CTTGAG	3 cut(s)	575, 883, 1625
Bsa29I	ATCGAT	1 cut(s)	17
BsaAI	YACGTR	1 cut(s)	2542
BsaBI	GATNNNNATC	3 cut(s)	404, 648, 2436
BsaJI	CCNNGG	4 cut(s)	82, 443, 583, 940
BsaWI	WCCGGW	3 cut(s)	33, 145, 303
BsaXI	ACNNNNNCTCC	2 cut(s)	157, 187
Bsc4I	CCNNNNNNNGG	7 cut(s)	33, 320, 500, 1481, 1579, 1895, 2474
Bse118I	RCCGGY	1 cut(s)	205
Bse1I	ACTGG	9 cut(s)	372, 749, 822, 1340, 1349, 1776, 1968, 2467, 2623
Bse21I	CCTNAGG	1 cut(s)	285
Bse3DI	GCAATG	3 cut(s)	423, 1540, 2742
Bse8I	GATNNNNATC	3 cut(s)	404, 648, 2436
BseAI	TCCGGA	2 cut(s)	33, 145
BseBI	CCWGG	3 cut(s)	444, 663, 1507
BseCI	ATCGAT	1 cut(s)	17
BseDI	CCNNGG	4 cut(s)	82, 443, 583, 940
BseJI	GATNNNNATC	3 cut(s)	404, 648, 2436
BseLI	CCNNNNNNNGG	7 cut(s)	33, 320, 500, 1481, 1579, 1895, 2474
BseMI	GCAATG	3 cut(s)	423, 1540, 2742
BseMII	CTCAG	6 cut(s)	171, 308, 704, 1817, 1966, 2178
BseNI	ACTGG	9 cut(s)	372, 749, 822, 1340, 1349, 1776, 1968, 2467, 2623
BseRI	GAGGAG	2 cut(s)	500, 572
BseX3I	CGGCCG	1 cut(s)	79
BseXI	GCAGC	6 cut(s)	16, 979, 1201, 1357, 1806, 2096
BseYI	CCCAGC	2 cut(s)	2526, 2530
BsgI	GTGCAG	2 cut(s)	1308, 1364
Bsh1236I	CGCG	2 cut(s)	101, 466
Bsh1285I	CGRYCG	1 cut(s)	82
BshFI	GGCC	5 cut(s)	81, 661, 834, 1075, 1950
BshNI	GGYRCC	4 cut(s)	187, 1001, 1789, 2597
BshVI	ATCGAT	1 cut(s)	17
BsiEI	CGRYCG	1 cut(s)	82
BsiSI	CCGG	5 cut(s)	34, 47, 146, 206, 304
BslFI	GGGAC	2 cut(s)	460, 1948
BslI	CCNNNNNNNGG	7 cut(s)	33, 320, 500, 1481, 1579, 1895, 2474
BsmAI	GTCTC	1 cut(s)	66
BsmBI	CGTCTC	1 cut(s)	66
BsmFI	GGGAC	2 cut(s)	460, 1948
BsmI	GAATGC	1 cut(s)	2648
BsnI	GGCC	5 cut(s)	81, 661, 834, 1075, 1950
Bsp119I	TTCGAA	1 cut(s)	1421
Bsp1286I	GDGCHC	1 cut(s)	1278
Bsp13I	TCCGGA	2 cut(s)	33, 145
Bsp1407I	TGTACA	1 cut(s)	338
Bsp19I	CCATGG	1 cut(s)	583
BspACI	CCGC	8 cut(s)	66, 101, 112, 176, 464, 701, 1690, 2250
BspANI	GGCC	5 cut(s)	81, 661, 834, 1075, 1950
BspCNI	CTCAG	6 cut(s)	172, 307, 705, 1816, 1965, 2179
BspDI	ATCGAT	1 cut(s)	17
BspEI	TCCGGA	2 cut(s)	33, 145
BspFNI	CGCG	2 cut(s)	101, 466
BspHI	TCATGA	2 cut(s)	2119, 2613
BspLI	GGNNCC	8 cut(s)	133, 166, 189, 325, 561, 1003, 1791, 2599
BspMI	ACCTGC	1 cut(s)	1483
BspOI	GCTAGC	1 cut(s)	609
BspPI	GGATC	6 cut(s)	13, 126, 139, 156, 662, 1715
BspT104I	TTCGAA	1 cut(s)	1421
BspT107I	GGYRCC	4 cut(s)	187, 1001, 1789, 2597
BsrBI	CCGCTC	1 cut(s)	112
BsrDI	GCAATG	3 cut(s)	423, 1540, 2742
BsrFI	RCCGGY	1 cut(s)	205
BsrGI	TGTACA	1 cut(s)	338
BsrI	ACTGG	9 cut(s)	372, 749, 822, 1340, 1349, 1776, 1968, 2467, 2623
BssAI	RCCGGY	1 cut(s)	205
BssECI	CCNNGG	4 cut(s)	82, 443, 583, 940
BssNAI	GTATAC	1 cut(s)	1141
BssT1I	CCWWGG	1 cut(s)	583
Bst1107I	GTATAC	1 cut(s)	1141
Bst2UI	CCWGG	3 cut(s)	444, 663, 1507
Bst4CI	ACNGT	6 cut(s)	343, 568, 1670, 1861, 1978, 2219
Bst6I	CTCTTC	2 cut(s)	238, 2496
BstAUI	TGTACA	1 cut(s)	338
BstBAI	YACGTR	1 cut(s)	2542
BstBI	TTCGAA	1 cut(s)	1421
BstC8I	GCNNGC	5 cut(s)	207, 220, 607, 1817, 2254
BstDSI	CCRYGG	3 cut(s)	82, 583, 940
BstFNI	CGCG	2 cut(s)	101, 466
BstH2I	RGCGCY	1 cut(s)	10
BstHHI	GCGC	1 cut(s)	9
BstMAI	GTCTC	1 cut(s)	66
BstMCI	CGRYCG	1 cut(s)	82
BstMWI	GCNNNNNNNGC	6 cut(s)	215, 472, 1001, 1211, 1223, 1295
BstNI	CCWGG	3 cut(s)	444, 663, 1507
BstNSI	RCATGY	3 cut(s)	2210, 2508, 2675
BstPAI	GACNNNNGTC	1 cut(s)	2198
BstSCI	CCNGG	3 cut(s)	442, 661, 1505
BstSFI	CTRYAG	1 cut(s)	2088
BstUI	CGCG	2 cut(s)	101, 466
BstV1I	GCAGC	6 cut(s)	16, 979, 1201, 1357, 1806, 2096
BstV2I	GAAGAC	2 cut(s)	562, 1410
BstX2I	RGATCY	5 cut(s)	131, 148, 1707, 1765, 2234
BstXI	CCANNNNNNTGG	1 cut(s)	2509
BstYI	RGATCY	5 cut(s)	131, 148, 1707, 1765, 2234
BstZ17I	GTATAC	1 cut(s)	1141
BstZI	CGGCCG	1 cut(s)	79
Bsu15I	ATCGAT	1 cut(s)	17
Bsu36I	CCTNAGG	1 cut(s)	285
BsuI	GTATCC	1 cut(s)	1397
BsuRI	GGCC	5 cut(s)	81, 661, 834, 1075, 1950
BsuTUI	ATCGAT	1 cut(s)	17
BtgI	CCRYGG	3 cut(s)	82, 583, 940
BtrI	CACGTC	2 cut(s)	194, 2036
BtsI	GCAGTG	3 cut(s)	1325, 1620, 2053
BtsIMutI	CAGTG	7 cut(s)	1201, 1298, 1325, 1347, 1620, 2053, 2601
BveI	ACCTGC	1 cut(s)	1483
Cac8I	GCNNGC	5 cut(s)	207, 220, 607, 1817, 2254
CaiI	CAGNNNCTG	5 cut(s)	202, 347, 1322, 1594, 2141
CciI	TCATGA	2 cut(s)	2119, 2613
CfoI	GCGC	1 cut(s)	9
Cfr10I	RCCGGY	1 cut(s)	205
Cfr13I	GGNCC	5 cut(s)	431, 1073, 1439, 1634, 2465
ClaI	ATCGAT	1 cut(s)	17
CseI	GACGC	1 cut(s)	1600
Csp6I	GTAC	6 cut(s)	339, 1309, 1454, 1485, 1644, 2628
CviAII	CATG	9 cut(s)	584, 1428, 1436, 1460, 2120, 2207, 2505, 2614, 2672
CviQI	GTAC	6 cut(s)	339, 1309, 1454, 1485, 1644, 2628
DraI	TTTAAA	3 cut(s)	787, 1173, 2026
DriI	GACNNNNNGTC	1 cut(s)	453
EaeI	YGGCCR	2 cut(s)	79, 832
EagI	CGGCCG	1 cut(s)	79
Eam1104I	CTCTTC	2 cut(s)	238, 2496
Eam1105I	GACNNNNNGTC	1 cut(s)	453
EarI	CTCTTC	2 cut(s)	238, 2496
EciI	GGCGGA	1 cut(s)	165
EclXI	CGGCCG	1 cut(s)	79
Eco130I	CCWWGG	1 cut(s)	583
Eco147I	AGGCCT	2 cut(s)	661, 1950
Eco24I	GRGCYC	1 cut(s)	1278
Eco32I	GATATC	1 cut(s)	364
Eco47I	GGWCC	4 cut(s)	431, 1439, 1634, 2465
Eco52I	CGGCCG	1 cut(s)	79
Eco57I	CTGAAG	1 cut(s)	1395
Eco81I	CCTNAGG	1 cut(s)	285
EcoRI	GAATTC	3 cut(s)	211, 477, 1423
EcoRII	CCWGG	3 cut(s)	442, 661, 1505
EcoRV	GATATC	1 cut(s)	364
EcoT14I	CCWWGG	1 cut(s)	583
EcoT22I	ATGCAT	3 cut(s)	258, 405, 1657
EcoT38I	GRGCYC	1 cut(s)	1278
ErhI	CCWWGG	1 cut(s)	583
Esp3I	CGTCTC	1 cut(s)	66
FaeI	CATG	9 cut(s)	587, 1431, 1439, 1463, 2123, 2210, 2508, 2617, 2675
FaqI	GGGAC	2 cut(s)	460, 1948
FatI	CATG	9 cut(s)	583, 1427, 1435, 1459, 2119, 2206, 2504, 2613, 2671
FauI	CCCGC	1 cut(s)	105
FbaI	TGATCA	2 cut(s)	982, 2059
FblI	GTMKAC	2 cut(s)	1122, 1140
Fnu4HI	GCNGC	7 cut(s)	5, 968, 1215, 1346, 1691, 1820, 2085
FriOI	GRGCYC	1 cut(s)	1278
Fsp4HI	GCNGC	7 cut(s)	5, 968, 1215, 1346, 1691, 1820, 2085
FspBI	CTAG	6 cut(s)	246, 458, 534, 606, 2159, 2417
GlaI	GCGC	1 cut(s)	8
GluI	GCNGC	7 cut(s)	5, 968, 1215, 1346, 1691, 1820, 2085
GsaI	CCCAGC	2 cut(s)	2530, 2534
GsuI	CTGGAG	2 cut(s)	1323, 2664
HaeII	RGCGCY	1 cut(s)	10
HaeIII	GGCC	5 cut(s)	81, 661, 834, 1075, 1950
HapII	CCGG	5 cut(s)	34, 47, 146, 206, 304
HgaI	GACGC	1 cut(s)	1600
HhaI	GCGC	1 cut(s)	9
Hin1II	CATG	9 cut(s)	587, 1431, 1439, 1463, 2123, 2210, 2508, 2617, 2675
Hin6I	GCGC	1 cut(s)	7
HinP1I	GCGC	1 cut(s)	7
HincII	GTYRAC	2 cut(s)	1123, 1974
HindII	GTYRAC	2 cut(s)	1123, 1974
HindIII	AAGCTT	1 cut(s)	2254
HpaI	GTTAAC	1 cut(s)	1974
HpaII	CCGG	5 cut(s)	34, 47, 146, 206, 304
HphI	GGTGA	4 cut(s)	677, 1236, 2390, 2432
Hpy166II	GTNNAC	9 cut(s)	353, 1123, 1141, 1456, 1487, 1974, 2193, 2390, 2575
Hpy8I	GTNNAC	9 cut(s)	353, 1123, 1141, 1456, 1487, 1974, 2193, 2390, 2575
Hpy99I	CGWCG	2 cut(s)	80, 131
HpyAV	CCTTC	7 cut(s)	37, 100, 178, 262, 651, 1985, 2641
HpyCH4III	ACNGT	6 cut(s)	343, 568, 1670, 1861, 1978, 2219
HpyCH4IV	ACGT	6 cut(s)	193, 454, 2035, 2409, 2541, 2726
HpyF10VI	GCNNNNNNNGC	6 cut(s)	215, 472, 1001, 1211, 1223, 1295
HpySE526I	ACGT	6 cut(s)	193, 454, 2035, 2409, 2541, 2726
Hsp92II	CATG	9 cut(s)	587, 1431, 1439, 1463, 2123, 2210, 2508, 2617, 2675
HspAI	GCGC	1 cut(s)	7
Kpn2I	TCCGGA	2 cut(s)	33, 145
KroI	GCCGGC	1 cut(s)	205
KroNI	GCCGGC	1 cut(s)	207
Ksp22I	TGATCA	2 cut(s)	982, 2059
KspAI	GTTAAC	1 cut(s)	1974
LmnI	GCTCC	5 cut(s)	109, 115, 559, 841, 2267
Lsp1109I	GCAGC	6 cut(s)	16, 979, 1201, 1357, 1806, 2096
MaeI	CTAG	6 cut(s)	246, 458, 534, 606, 2159, 2417
MaeII	ACGT	6 cut(s)	193, 454, 2035, 2409, 2541, 2726
MaeIII	GTNAC	7 cut(s)	1039, 1242, 1616, 1672, 2096, 2245, 2537
MbiI	CCGCTC	1 cut(s)	112
MfeI	CAATTG	1 cut(s)	1331
MflI	RGATCY	5 cut(s)	131, 148, 1707, 1765, 2234
MhlI	GDGCHC	1 cut(s)	1278
MlsI	TGGCCA	1 cut(s)	834
MluNI	TGGCCA	1 cut(s)	834
MlyI	GAGTC	3 cut(s)	224, 1330, 1930
MmeI	TCCRAC	2 cut(s)	109, 934
Mox20I	TGGCCA	1 cut(s)	834
Mph1103I	ATGCAT	3 cut(s)	258, 405, 1657
MroI	TCCGGA	2 cut(s)	33, 145
MroNI	GCCGGC	1 cut(s)	205
MroXI	GAANNNNTTC	1 cut(s)	1928
MscI	TGGCCA	1 cut(s)	834
MslI	CAYNNNNRTG	1 cut(s)	306
Msp20I	TGGCCA	1 cut(s)	834
MspA1I	CMGCKG	3 cut(s)	970, 2087, 2530
MspI	CCGG	5 cut(s)	34, 47, 146, 206, 304
MspR9I	CCNGG	3 cut(s)	444, 663, 1507
MunI	CAATTG	1 cut(s)	1331
Mva1269I	GAATGC	1 cut(s)	2648
MvaI	CCWGG	3 cut(s)	444, 663, 1507
MvnI	CGCG	2 cut(s)	101, 466
MwoI	GCNNNNNNNGC	6 cut(s)	215, 472, 1001, 1211, 1223, 1295
NaeI	GCCGGC	1 cut(s)	207
NcoI	CCATGG	1 cut(s)	583
NgoMIV	GCCGGC	1 cut(s)	205
NheI	GCTAGC	1 cut(s)	605
NlaIII	CATG	9 cut(s)	587, 1431, 1439, 1463, 2123, 2210, 2508, 2617, 2675
NlaIV	GGNNCC	8 cut(s)	133, 166, 189, 325, 561, 1003, 1791, 2599
NmuCI	GTSAC	4 cut(s)	1039, 1242, 1672, 2096
NsiI	ATGCAT	3 cut(s)	258, 405, 1657
NspI	RCATGY	3 cut(s)	2210, 2508, 2675
NspV	TTCGAA	1 cut(s)	1421
PagI	TCATGA	2 cut(s)	2119, 2613
PceI	AGGCCT	2 cut(s)	661, 1950
PciI	ACATGT	1 cut(s)	2504
PctI	GAATGC	1 cut(s)	2648
PdiI	GCCGGC	1 cut(s)	207
PdmI	GAANNNNTTC	1 cut(s)	1928
PflMI	CCANNNNNTGG	2 cut(s)	320, 1895
PkrI	GCNGC	7 cut(s)	6, 969, 1216, 1347, 1692, 1821, 2086
PleI	GAGTC	3 cut(s)	224, 1330, 1930
PpsI	GAGTC	3 cut(s)	224, 1330, 1930
Ppu21I	YACGTR	1 cut(s)	2542
PscI	ACATGT	1 cut(s)	2504
PshAI	GACNNNNGTC	1 cut(s)	2198
PshBI	ATTAAT	2 cut(s)	384, 1740
PsiI	TTATAA	1 cut(s)	1563
Psp6I	CCWGG	3 cut(s)	442, 661, 1505
PspFI	CCCAGC	2 cut(s)	2526, 2530
PspGI	CCWGG	3 cut(s)	442, 661, 1505
PspN4I	GGNNCC	8 cut(s)	133, 166, 189, 325, 561, 1003, 1791, 2599
PspPI	GGNCC	5 cut(s)	431, 1073, 1439, 1634, 2465
PstNI	CAGNNNCTG	5 cut(s)	202, 347, 1322, 1594, 2141
PsuI	RGATCY	5 cut(s)	131, 148, 1707, 1765, 2234
PvuII	CAGCTG	3 cut(s)	970, 2087, 2530
RsaI	GTAC	6 cut(s)	340, 1310, 1455, 1486, 1645, 2629
RsaNI	GTAC	6 cut(s)	339, 1309, 1454, 1485, 1644, 2628
RseI	CAYNNNNRTG	1 cut(s)	306
SalI	GTCGAC	1 cut(s)	1121
SatI	GCNGC	7 cut(s)	5, 968, 1215, 1346, 1691, 1820, 2085
Sau96I	GGNCC	5 cut(s)	431, 1073, 1439, 1634, 2465
ScaI	AGTACT	1 cut(s)	1310
SchI	GAGTC	3 cut(s)	224, 1330, 1930
ScrFI	CCNGG	3 cut(s)	444, 663, 1507
SduI	GDGCHC	1 cut(s)	1278
SfcI	CTRYAG	1 cut(s)	2088
SfuI	TTCGAA	1 cut(s)	1421
SinI	GGWCC	4 cut(s)	431, 1439, 1634, 2465
SmiMI	CAYNNNNRTG	1 cut(s)	306
SmlI	CTYRAG	3 cut(s)	554, 862, 1604
SmoI	CTYRAG	3 cut(s)	554, 862, 1604
SseBI	AGGCCT	2 cut(s)	661, 1950
SsiI	CCGC	8 cut(s)	66, 101, 112, 176, 464, 701, 1690, 2250
SspI	AATATT	2 cut(s)	1069, 2347
SspMI	CTAG	6 cut(s)	246, 458, 534, 606, 2159, 2417
StuI	AGGCCT	2 cut(s)	661, 1950
StyD4I	CCNGG	3 cut(s)	442, 661, 1505
StyI	CCWWGG	1 cut(s)	583
TaaI	ACNGT	6 cut(s)	343, 568, 1670, 1861, 1978, 2219
TaiI	ACGT	6 cut(s)	196, 457, 2038, 2412, 2544, 2729
TatI	WGTACW	2 cut(s)	338, 1308
TauI	GCSGC	1 cut(s)	1693
TscAI	CASTG	7 cut(s)	1201, 1305, 1332, 1347, 1627, 2053, 2608
TseFI	GTSAC	4 cut(s)	1039, 1242, 1672, 2096
TseI	GCWGC	6 cut(s)	4, 967, 1214, 1345, 1819, 2084
Tsp45I	GTSAC	4 cut(s)	1039, 1242, 1672, 2096
TspDTI	ATGAA	9 cut(s)	281, 324, 485, 828, 1076, 1278, 1416, 2004, 2718
TspGWI	ACGGA	4 cut(s)	929, 973, 1158, 2663
TspRI	CASTG	7 cut(s)	1201, 1305, 1332, 1347, 1627, 2053, 2608
Van91I	CCANNNNNTGG	2 cut(s)	320, 1895
VpaK11BI	GGWCC	4 cut(s)	431, 1439, 1634, 2465
VspI	ATTAAT	2 cut(s)	384, 1740
XapI	RAATTY	4 cut(s)	211, 477, 509, 1423
XceI	RCATGY	3 cut(s)	2210, 2508, 2675
XmiI	GTMKAC	2 cut(s)	1122, 1140
XmnI	GAANNNNTTC	1 cut(s)	1928
XspI	CTAG	6 cut(s)	246, 458, 534, 606, 2159, 2417
ZrmI	AGTACT	1 cut(s)	1310
Zsp2I	ATGCAT	3 cut(s)	258, 405, 1657

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.