Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0014491

Physical Location & Seq

Genomic Coordinates Reverse (-)

88192023 .. 88199882

Gene Structure 7860 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00150840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3216 bp

ATGCAGCGCCAGAAATCGATCCTCTCCTTCTTCCGGAAACCCTCGCCAGACAAAAGCACCTCCACCGCCGGAGACGACGGCCATGGAGTTAATCAGTTCGCGGCTAAGGAGCAGGAGCAGAAGCCCATCGGATCCAATCAGCCTCCGGATCTCGAAGTTCGAGGAACCGACACTCCGCCGGAGAAGGTGCCACGTCAGATTCTGCCGGCGAATTCTGCGAGCCTGAAAGACTCTTCGCCTTTCTCTAGTATTATGCATAAGTTTATGAAGGTCGATGACACACATAAGGCTTCTCAGAGCCACCGGAATGAACCAAATGGTGGTTCCTCTAATGATTTTACAGTATCTGGTAAACTCAATGATACCAATTCCAGTAAAAAAATTAATCGAGGTGCTGTGATGCATATCGAAAGTGATGAGGACATTTCTGGACCAGAAACACCGGGGACACAACGTCTAGTACCACGTATGAAGCGAATTCAAGAGGAGATTCCTAAGTTTGGGGATAAATTTGGGAGTTCTTTGCCAGATTCTAGCAAAAGATTGAAAGTTCTTGAGGAGCCAACAGTCTTAGACAAGAGCCATGGGGAAGCATCTGAAATGGCTAGCAAGTTTGAGTGGCTTGACCCATCCCGAATCAGAGATGCAAATCGCAGAAGGCCTGGTGATCCTCTCTATGATAAAACATCACTTTTTATACCGCCTGATGCGCTGAAGAAAATGTCAGCATCACAAAGACAATACTGGGATGTCAAGTGTCAGTATATGGATGTTGTGATTTTCTTTAAAGTGGGAAAGTTTTATGAGCTTTATGAACTAGATGCTGAAATTGGCCACAAGGAGCTTGATTGGAAAATGACCTTGAGTGGTGTTGGGAAATGTCGTCAGGTTGGCATCTCTGAAAGTGGGATTGACGATGCTGTTCAAAAGTTGGTTGTCCGTGGGTATAAAGTTGGACGGATAGAGCAGCTGGAAACTTCTGATCAAGTAAAAGCCAGAGGTGCCAAAGCTGTGATTCCAAGAAAATTGGTTCAGGTAGTCACACCATCAACCACAACTGATGGAAATATTGGGCCTGATGCTGTTCATCTTCTTGCAATAAAAGAGGGGAACTCTGGGGTTGACAATGGTTCAGTTGTATACGGGTTTGCTTTTGTTGATTGTTCTGCTTTAAAGTTCTGGATTGGTGCTATCAGTGATGATGCTTCGTGTGCTGCTTTGGGGGCTTTATTGATGCAAGTGTCACCCAAAGAAGTTGTATATGAAAACAGAGGGCTCTCAAAAGAAGCTCAAAAAGCACTGAAAAAGTACTCAACAGGTTCTGCAATACAATTGACTCCAGTGCAGCCAGTCAATGATTTTATGGATGCTTCTGAAGTTAGTAAATTGATTCAGTTGAAAGGATACTTCAAAGGGTCTTTGAATTCATGGAATCATGGACCTGATGGTGTGGTACACCATGATATAACTTTACCTGCTCTGGGTACACTTATTGACCACCTTTCCAGGTTGATGTTAGATGATGTTTTACGCAACGGAGATATATTACCTTACCAAGTTTATAAGGGTTGCCTCAAAATGGATGGACAGACGCTGATAAATCTTGAGATTTTCAGTAATACTGCTGATGGTGGTACGCTGTACGCATATCTCGATAACTGTGTGACTGCATCTGGTAAGCGCCTTTTGAGGAAGTGGATCTGCCATCCCTTGAAGTCTGTTGAAGCTATTAATAACAGGCTTAATGTGGTTGAAGATCTACTGGCACATCCAGAAATGGTGCCACTTATTGCTCAGTATCTCCGCAAGCTTCCAGACTTAGAGAGGTTGTTTGGACGGGTTAGAGCGACTGTTCAGTCGTCAGCTTCCCTCCTATTGCCGTTATTTGGCAAGAAATTACTAAAACAGAGAGTGAAAGTGTTTGGGACTCTTGTGAAAGGCCTCAGATTTGGACTGGATTTGTTAACGGTATTACAAAATGAAGGGCATATAATTGAGCTGTTATTAAAACTATTTAAAGTTCCACATCTAAGTGGCAGTGATGGGCTTGATCAATATCTTAAGCAATTTGAAGCAGCTGTAGATAGTGACTTCCCAAATTACCAGAATCATGATGCAACCGATTCAGATGCTGAAATACTCTCGATTCTAGTCGAGCTATTCATGGAGAAAGCAGCTGAGTGGACTGATGTCATACATGCCATCAACTGTATTGATGTGCTAAGATCTTTTGTAGTTAGCGCAAGCTTCCCAAGTGGAGCTATGTCCAGACCTGTTCTTTTGCCTCAATCTCAAAATACAACCTTGAATGGAGAAAATGGAAGTCCTACACTTAATATTAACGGACTATGGCATCCATTTGCTCTTGGTGAAAATGGTGGACAGCCTGTGCCAAATGATGTAGTTCTAGGTGAAAATACTGATGGCTATCATCCACGCACTTTACTATTAACTGGACCAAATATGGGTGGGAAATCAACTCTTCTTCGTTCCACATGTCTGGCTGTTATACTTGCCCAGCTGGGCTGTTACGTGCCTTGTGAGATATGTGTTCTCTCACTTGTGGACATCATCTTTACACGGCTTGGTGCCACTGATCGTATCATGACTGGCGAGAGTACCTTCTTAGTGGAATGCACGGAAACGGCATCAGTTCTTCAACATGCCACTCCAGATTCTTTGGTTATTCTTGATGAATTGGGTCGTGGAACAAGCACGTTTGATGGATATGCCATTGCATATGCTGTATTTCGTCATCTTGTCGAAAAGATCAACTGCAGACTGTTATTCGCCACACACTATCACCCGCTTACGAGAGAATTTGCTTCTCATCCGCATGTTTCATTGCAACACATGGCATGTGCTTTTAGGTCGAAATCTAAATCCCCTTCAAAAAGTGACCAAGAGCTGGTATTTCTCTATCGCCTTACCTCTGGGGCATGCCCAGAGAGTTATGGATTGCAAGTAGCAATGATGGCTGGGATCCCTGAGAATGTGGTTGAAGCTGCTTCTAAGGCTGGCCAAGTAATGAAGAAATCTGTTGGAGAAAGCTTCAGGTCCAGTGAACAGAGATCGGAATTCTCAACTCTCCATGAGGAGTGGCTGAAGACACTGTTAACTGTTTCACAAACCGGTAATTATAACGACGAAGATTCATTGGACGTATTGTTTTGCTTGCAGCAGGAACTAAAGTGCTCATATAAATTGGCCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000217 GO:0000404 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006259 GO:0006281 GO:0006298 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008094 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030983 GO:0032135 GO:0032300 GO:0032991 GO:0033554 GO:0034641 GO:0042623 GO:0043170 GO:0043570 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990391

KEGG Pathways

Metabolic & Signaling

ko01524 ko03430 ko05200 ko05210 map01524 map03430 map05200 map05210

Pfam Domains

Protein Families

MutS_I MutS_II MutS_III MutS_V

Protein Analysis

1071

Amino Acids

118.04

Weight (kDa)

6.3

Isoelectric Point (pI)

38.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MutS_I	PF01624	244 - 355	2.6e-29	MutS domain I
MutS_II	PF05188	364 - 509	1.2e-11	MutS domain II
MutS_III	PF05192	530 - 744	5.2e-32	MutS domain III
MutS_V	PF00488	816 - 1008	7.7e-74	MutS domain V

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014491)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24495 AT3G24495
fragaria_vesca	FvH4_1g05130 FvH4_1g05130
malus_domestica	MD15G1187500.v1.1
prunus_persica	Prupe.7G228900_v2.0.a1
pyrus_communis	pycom15g16830
rosa_chinensis	RchiOBHm_Chr2g0090761
rosa_laevigata	RLG00000016141
rosa_multiflora	Rmu_ssc0000367.1_g000007
rosa_roxburghii	Rroxscaffold_2G00150840
rosa_rugosa	Rorug02G0010300
rosa_samantha	Rh2AG056300 Rh2BG055000 Rh2CG057400 Rh2DG055900
rosa_wichuraiana	Rw2G004620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1563, 3141
AasI	GACNNNNNNGTC	1 cut(s)	1855
Acc36I	ACCTGC	1 cut(s)	1483
AccB1I	GGYRCC	4 cut(s)	187, 1001, 1780, 2588
AccB7I	CCANNNNNTGG	2 cut(s)	320, 2908
AccI	GTMKAC	1 cut(s)	1140
AccII	CGCG	1 cut(s)	101
AccIII	TCCGGA	2 cut(s)	33, 145
AciI	CCGC	7 cut(s)	66, 101, 176, 701, 1804, 2807, 2834
AclWI	GGATC	8 cut(s)	13, 126, 139, 156, 662, 1706, 2977, 2990
AcoI	YGGCCR	4 cut(s)	79, 832, 3019, 3207
AcsI	RAATTY	6 cut(s)	211, 477, 509, 1423, 2819, 3077
AcuI	CTGAAG	4 cut(s)	734, 1395, 3037, 3125
AfaI	GTAC	7 cut(s)	462, 1310, 1455, 1486, 1636, 1643, 2620
AfiI	CCNNNNNNNGG	8 cut(s)	33, 320, 500, 1481, 1579, 1886, 2465, 2908
AflII	CTTAAG	1 cut(s)	2060
AflIII	ACRYGT	1 cut(s)	2495
AgeI	ACCGGT	1 cut(s)	3131
AhdI	GACNNNNNGTC	1 cut(s)	453
AjiI	CACGTC	1 cut(s)	194
AjnI	CCWGG	2 cut(s)	661, 1505
AjuI	GAANNNNNNNTTGG	2 cut(s)	307, 339
AloI	GAACNNNNNNTCC	2 cut(s)	157, 189
Alw21I	GWGCWC	1 cut(s)	3197
Alw26I	GTCTC	1 cut(s)	66
AlwI	GGATC	8 cut(s)	13, 126, 139, 156, 662, 1706, 2977, 2990
AlwNI	CAGNNNCTG	5 cut(s)	202, 347, 1322, 1594, 2132
Aor13HI	TCCGGA	2 cut(s)	33, 145
AoxI	GGCC	7 cut(s)	79, 659, 832, 1073, 1939, 3019, 3207
ApeKI	GCWGC	8 cut(s)	4, 967, 1214, 1345, 2075, 2174, 3005, 3178
ApoI	RAATTY	6 cut(s)	211, 477, 509, 1423, 2819, 3077
AseI	ATTAAT	2 cut(s)	384, 1731
AsiGI	ACCGGT	1 cut(s)	3131
AspLEI	GCGC	4 cut(s)	9, 712, 1683, 2243
AspS9I	GGNCC	5 cut(s)	431, 1073, 1439, 2456, 3057
AsuC2I	CCSGG	1 cut(s)	444
AsuHPI	GGTGA	5 cut(s)	677, 1236, 2381, 2423, 2795
AsuNHI	GCTAGC	1 cut(s)	605
AvaII	GGWCC	4 cut(s)	431, 1439, 2456, 3057
BalI	TGGCCA	3 cut(s)	834, 3021, 3209
BamHI	GGATCC	2 cut(s)	131, 2982
BanI	GGYRCC	4 cut(s)	187, 1001, 1780, 2588
BanII	GRGCYC	1 cut(s)	1278
BbsI	GAAGAC	1 cut(s)	3113
Bbv12I	GWGCWC	1 cut(s)	3197
BbvI	GCAGC	8 cut(s)	16, 979, 1201, 1357, 2087, 2186, 2992, 3190
BceAI	ACGGC	4 cut(s)	94, 1864, 2597, 2661
BcgI	CGANNNNNNTGC	2 cut(s)	2111, 2145
BciT130I	CCWGG	2 cut(s)	663, 1507
BciVI	GTATCC	1 cut(s)	1397
BclI	TGATCA	2 cut(s)	982, 2050
BcnI	CCSGG	1 cut(s)	444
BcoDI	GTCTC	1 cut(s)	66
BfaI	CTAG	7 cut(s)	246, 458, 534, 606, 818, 2150, 2408
BfmI	CTRYAG	2 cut(s)	2079, 2776
BfoI	RGCGCY	2 cut(s)	10, 1684
BfrI	CTTAAG	1 cut(s)	2060
BfuAI	ACCTGC	1 cut(s)	1483
BfuI	GTATCC	1 cut(s)	1397
BglII	AGATCT	2 cut(s)	1756, 2225
BisI	GCNGC	9 cut(s)	5, 102, 968, 1215, 1346, 2076, 2175, 3006, 3179
BlsI	GCNGC	9 cut(s)	6, 103, 969, 1216, 1347, 2077, 2176, 3007, 3180
BmcAI	AGTACT	1 cut(s)	1310
Bme1390I	CCNGG	3 cut(s)	444, 663, 1507
Bme18I	GGWCC	4 cut(s)	431, 1439, 2456, 3057
BmeRI	GACNNNNNGTC	1 cut(s)	453
BmgBI	CACGTC	1 cut(s)	194
BmgT120I	GGNCC	5 cut(s)	431, 1073, 1439, 2456, 3057
BmiI	GGNNCC	9 cut(s)	133, 166, 189, 325, 561, 1003, 1782, 2590, 2984
BmrFI	CCNGG	3 cut(s)	444, 663, 1507
BmrI	ACTGGG	1 cut(s)	754
BmtI	GCTAGC	1 cut(s)	609
BmuI	ACTGGG	1 cut(s)	754
BoxI	GACNNNNGTC	1 cut(s)	2189
BpiI	GAAGAC	1 cut(s)	3113
BplI	GAGNNNNNCTC	2 cut(s)	1097, 1129
BpmI	CTGGAG	2 cut(s)	1323, 2655
Bpu10I	CCTNAGC	1 cut(s)	105
BpuEI	CTTGAG	3 cut(s)	575, 883, 1625
BpuMI	CCSGG	1 cut(s)	444
Bsa29I	ATCGAT	1 cut(s)	17
BsaAI	YACGTR	2 cut(s)	467, 2533
BsaBI	GATNNNNATC	4 cut(s)	404, 648, 2055, 2427
BsaJI	CCNNGG	4 cut(s)	82, 443, 583, 940
BsaWI	WCCGGW	4 cut(s)	33, 145, 303, 3131
BsaXI	ACNNNNNCTCC	2 cut(s)	157, 187
Bsc4I	CCNNNNNNNGG	8 cut(s)	33, 320, 500, 1481, 1579, 1886, 2465, 2908
Bse118I	RCCGGY	2 cut(s)	205, 3131
Bse1I	ACTGG	9 cut(s)	372, 749, 1340, 1349, 1767, 1959, 2458, 2614, 3060
Bse3DI	GCAATG	3 cut(s)	2733, 2843, 2976
Bse8I	GATNNNNATC	4 cut(s)	404, 648, 2055, 2427
BseAI	TCCGGA	2 cut(s)	33, 145
BseBI	CCWGG	2 cut(s)	663, 1507
BseCI	ATCGAT	1 cut(s)	17
BseDI	CCNNGG	4 cut(s)	82, 443, 583, 940
BseJI	GATNNNNATC	4 cut(s)	404, 648, 2055, 2427
BseLI	CCNNNNNNNGG	8 cut(s)	33, 320, 500, 1481, 1579, 1886, 2465, 2908
BseMI	GCAATG	3 cut(s)	2733, 2843, 2976
BseMII	CTCAG	5 cut(s)	308, 1808, 1957, 2169, 2979
BseNI	ACTGG	9 cut(s)	372, 749, 1340, 1349, 1767, 1959, 2458, 2614, 3060
BseRI	GAGGAG	3 cut(s)	500, 572, 3110
BseXI	GCAGC	8 cut(s)	16, 979, 1201, 1357, 2087, 2186, 2992, 3190
BseYI	CCCAGC	3 cut(s)	2517, 2521, 2978
BsgI	GTGCAG	1 cut(s)	1364
Bsh1236I	CGCG	1 cut(s)	101
BshFI	GGCC	7 cut(s)	81, 661, 834, 1075, 1941, 3021, 3209
BshNI	GGYRCC	4 cut(s)	187, 1001, 1780, 2588
BshTI	ACCGGT	1 cut(s)	3131
BshVI	ATCGAT	1 cut(s)	17
BsiHKAI	GWGCWC	1 cut(s)	3197
BsiSI	CCGG	8 cut(s)	34, 69, 146, 179, 206, 304, 443, 3132
BslFI	GGGAC	2 cut(s)	460, 1939
BslI	CCNNNNNNNGG	8 cut(s)	33, 320, 500, 1481, 1579, 1886, 2465, 2908
BsmAI	GTCTC	1 cut(s)	66
BsmBI	CGTCTC	1 cut(s)	66
BsmFI	GGGAC	2 cut(s)	460, 1939
BsmI	GAATGC	1 cut(s)	2639
BsnI	GGCC	7 cut(s)	81, 661, 834, 1075, 1941, 3021, 3209
Bsp1286I	GDGCHC	2 cut(s)	1278, 3197
Bsp13I	TCCGGA	2 cut(s)	33, 145
Bsp19I	CCATGG	2 cut(s)	82, 583
BspACI	CCGC	7 cut(s)	66, 101, 176, 701, 1804, 2807, 2834
BspANI	GGCC	7 cut(s)	81, 661, 834, 1075, 1941, 3021, 3209
BspCNI	CTCAG	5 cut(s)	307, 1807, 1956, 2170, 2980
BspDI	ATCGAT	1 cut(s)	17
BspEI	TCCGGA	2 cut(s)	33, 145
BspFNI	CGCG	1 cut(s)	101
BspHI	TCATGA	2 cut(s)	2110, 2604
BspLI	GGNNCC	9 cut(s)	133, 166, 189, 325, 561, 1003, 1782, 2590, 2984
BspMAI	CTGCAG	1 cut(s)	2780
BspMI	ACCTGC	1 cut(s)	1483
BspOI	GCTAGC	1 cut(s)	609
BspPI	GGATC	8 cut(s)	13, 126, 139, 156, 662, 1706, 2977, 2990
BspT107I	GGYRCC	4 cut(s)	187, 1001, 1780, 2588
BspTI	CTTAAG	1 cut(s)	2060
BsrDI	GCAATG	3 cut(s)	2733, 2843, 2976
BsrFI	RCCGGY	2 cut(s)	205, 3131
BsrI	ACTGG	9 cut(s)	372, 749, 1340, 1349, 1767, 1959, 2458, 2614, 3060
BssAI	RCCGGY	2 cut(s)	205, 3131
BssECI	CCNNGG	4 cut(s)	82, 443, 583, 940
BssNAI	GTATAC	1 cut(s)	1141
BssT1I	CCWWGG	2 cut(s)	82, 583
Bst1107I	GTATAC	1 cut(s)	1141
Bst2UI	CCWGG	2 cut(s)	663, 1507
Bst4CI	ACNGT	9 cut(s)	343, 568, 1661, 1852, 1969, 2210, 2784, 3114, 3121
Bst6I	CTCTTC	2 cut(s)	238, 2487
BstAFI	CTTAAG	1 cut(s)	2060
BstBAI	YACGTR	2 cut(s)	467, 2533
BstC8I	GCNNGC	8 cut(s)	207, 220, 607, 1808, 2245, 2941, 3019, 3176
BstDSI	CCRYGG	3 cut(s)	82, 583, 940
BstFNI	CGCG	1 cut(s)	101
BstH2I	RGCGCY	2 cut(s)	10, 1684
BstHHI	GCGC	4 cut(s)	9, 712, 1683, 2243
BstMAI	GTCTC	1 cut(s)	66
BstMWI	GCNNNNNNNGC	8 cut(s)	215, 709, 1001, 1211, 1223, 1295, 2975, 3014
BstNI	CCWGG	2 cut(s)	663, 1507
BstNSI	RCATGY	6 cut(s)	2201, 2499, 2666, 2840, 2862, 2943
BstPAI	GACNNNNGTC	1 cut(s)	2189
BstSCI	CCNGG	3 cut(s)	442, 661, 1505
BstSFI	CTRYAG	2 cut(s)	2079, 2776
BstUI	CGCG	1 cut(s)	101
BstV1I	GCAGC	8 cut(s)	16, 979, 1201, 1357, 2087, 2186, 2992, 3190
BstV2I	GAAGAC	1 cut(s)	3113
BstX2I	RGATCY	6 cut(s)	131, 148, 1698, 1756, 2225, 2982
BstXI	CCANNNNNNTGG	1 cut(s)	2500
BstYI	RGATCY	6 cut(s)	131, 148, 1698, 1756, 2225, 2982
BstZ17I	GTATAC	1 cut(s)	1141
Bsu15I	ATCGAT	1 cut(s)	17
BsuI	GTATCC	1 cut(s)	1397
BsuRI	GGCC	7 cut(s)	81, 661, 834, 1075, 1941, 3021, 3209
BsuTUI	ATCGAT	1 cut(s)	17
BtgI	CCRYGG	3 cut(s)	82, 583, 940
BtrI	CACGTC	1 cut(s)	194
BtsI	GCAGTG	1 cut(s)	2044
BtsIMutI	CAGTG	7 cut(s)	1201, 1298, 1347, 2044, 2592, 3067, 3110
BveI	ACCTGC	1 cut(s)	1483
Cac8I	GCNNGC	8 cut(s)	207, 220, 607, 1808, 2245, 2941, 3019, 3176
CaiI	CAGNNNCTG	5 cut(s)	202, 347, 1322, 1594, 2132
CciI	TCATGA	2 cut(s)	2110, 2604
CfoI	GCGC	4 cut(s)	9, 712, 1683, 2243
Cfr10I	RCCGGY	2 cut(s)	205, 3131
Cfr13I	GGNCC	5 cut(s)	431, 1073, 1439, 2456, 3057
ClaI	ATCGAT	1 cut(s)	17
CseI	GACGC	1 cut(s)	1600
Csp6I	GTAC	7 cut(s)	461, 1309, 1454, 1485, 1635, 1642, 2619
CspAI	ACCGGT	1 cut(s)	3131
CviQI	GTAC	7 cut(s)	461, 1309, 1454, 1485, 1635, 1642, 2619
DraI	TTTAAA	3 cut(s)	787, 1173, 2017
DrdI	GACNNNNNNGTC	1 cut(s)	1855
DriI	GACNNNNNGTC	1 cut(s)	453
DseDI	GACNNNNNNGTC	1 cut(s)	1855
EaeI	YGGCCR	4 cut(s)	79, 832, 3019, 3207
Eam1104I	CTCTTC	2 cut(s)	238, 2487
Eam1105I	GACNNNNNGTC	1 cut(s)	453
EarI	CTCTTC	2 cut(s)	238, 2487
EciI	GGCGGA	1 cut(s)	165
Eco130I	CCWWGG	2 cut(s)	82, 583
Eco147I	AGGCCT	2 cut(s)	661, 1941
Eco24I	GRGCYC	1 cut(s)	1278
Eco47I	GGWCC	4 cut(s)	431, 1439, 2456, 3057
Eco57I	CTGAAG	4 cut(s)	734, 1395, 3037, 3125
EcoRI	GAATTC	4 cut(s)	211, 477, 1423, 3077
EcoRII	CCWGG	2 cut(s)	661, 1505
EcoT14I	CCWWGG	2 cut(s)	82, 583
EcoT22I	ATGCAT	2 cut(s)	258, 405
EcoT38I	GRGCYC	1 cut(s)	1278
ErhI	CCWWGG	2 cut(s)	82, 583
Esp3I	CGTCTC	1 cut(s)	66
FaqI	GGGAC	2 cut(s)	460, 1939
FauI	CCCGC	1 cut(s)	2814
FauNDI	CATATG	1 cut(s)	2740
FbaI	TGATCA	2 cut(s)	982, 2050
FblI	GTMKAC	1 cut(s)	1140
Fnu4HI	GCNGC	9 cut(s)	5, 102, 968, 1215, 1346, 2076, 2175, 3006, 3179
FriOI	GRGCYC	1 cut(s)	1278
Fsp4HI	GCNGC	9 cut(s)	5, 102, 968, 1215, 1346, 2076, 2175, 3006, 3179
FspBI	CTAG	7 cut(s)	246, 458, 534, 606, 818, 2150, 2408
GlaI	GCGC	4 cut(s)	8, 711, 1682, 2242
GluI	GCNGC	9 cut(s)	5, 102, 968, 1215, 1346, 2076, 2175, 3006, 3179
GsaI	CCCAGC	3 cut(s)	2521, 2525, 2982
GsuI	CTGGAG	2 cut(s)	1323, 2655
HaeII	RGCGCY	2 cut(s)	10, 1684
HaeIII	GGCC	7 cut(s)	81, 661, 834, 1075, 1941, 3021, 3209
HapII	CCGG	8 cut(s)	34, 69, 146, 179, 206, 304, 443, 3132
HgaI	GACGC	1 cut(s)	1600
HhaI	GCGC	4 cut(s)	9, 712, 1683, 2243
Hin6I	GCGC	4 cut(s)	7, 710, 1681, 2241
HinP1I	GCGC	4 cut(s)	7, 710, 1681, 2241
HincII	GTYRAC	3 cut(s)	1123, 1965, 3117
HindII	GTYRAC	3 cut(s)	1123, 1965, 3117
HindIII	AAGCTT	3 cut(s)	1808, 2245, 3049
HpaI	GTTAAC	2 cut(s)	1965, 3117
HpaII	CCGG	8 cut(s)	34, 69, 146, 179, 206, 304, 443, 3132
HphI	GGTGA	5 cut(s)	677, 1236, 2381, 2423, 2795
Hpy99I	CGWCG	2 cut(s)	80, 3149
HpyAV	CCTTC	7 cut(s)	37, 178, 262, 651, 1976, 2632, 2898
HpyCH4III	ACNGT	9 cut(s)	343, 568, 1661, 1852, 1969, 2210, 2784, 3114, 3121
HpyCH4IV	ACGT	6 cut(s)	193, 454, 466, 2532, 2717, 3162
HpyF10VI	GCNNNNNNNGC	8 cut(s)	215, 709, 1001, 1211, 1223, 1295, 2975, 3014
HpySE526I	ACGT	6 cut(s)	193, 454, 466, 2532, 2717, 3162
HspAI	GCGC	4 cut(s)	7, 710, 1681, 2241
Kpn2I	TCCGGA	2 cut(s)	33, 145
KroI	GCCGGC	1 cut(s)	205
KroNI	GCCGGC	1 cut(s)	207
Ksp22I	TGATCA	2 cut(s)	982, 2050
KspAI	GTTAAC	2 cut(s)	1965, 3117
LmnI	GCTCC	5 cut(s)	109, 115, 559, 841, 2258
Lsp1109I	GCAGC	8 cut(s)	16, 979, 1201, 1357, 2087, 2186, 2992, 3190
MaeI	CTAG	7 cut(s)	246, 458, 534, 606, 818, 2150, 2408
MaeII	ACGT	6 cut(s)	193, 454, 466, 2532, 2717, 3162
MaeIII	GTNAC	6 cut(s)	1039, 1242, 1663, 2087, 2528, 2897
MfeI	CAATTG	1 cut(s)	1331
MflI	RGATCY	6 cut(s)	131, 148, 1698, 1756, 2225, 2982
MhlI	GDGCHC	2 cut(s)	1278, 3197
MlsI	TGGCCA	3 cut(s)	834, 3021, 3209
MluNI	TGGCCA	3 cut(s)	834, 3021, 3209
MlyI	GAGTC	3 cut(s)	224, 1330, 1921
MmeI	TCCRAC	2 cut(s)	934, 3022
Mox20I	TGGCCA	3 cut(s)	834, 3021, 3209
Mph1103I	ATGCAT	2 cut(s)	258, 405
MroI	TCCGGA	2 cut(s)	33, 145
MroNI	GCCGGC	1 cut(s)	205
MscI	TGGCCA	3 cut(s)	834, 3021, 3209
MslI	CAYNNNNRTG	3 cut(s)	306, 2031, 2835
Msp20I	TGGCCA	3 cut(s)	834, 3021, 3209
MspA1I	CMGCKG	4 cut(s)	970, 2078, 2177, 2521
MspCI	CTTAAG	1 cut(s)	2060
MspI	CCGG	8 cut(s)	34, 69, 146, 179, 206, 304, 443, 3132
MspR9I	CCNGG	3 cut(s)	444, 663, 1507
MunI	CAATTG	1 cut(s)	1331
Mva1269I	GAATGC	1 cut(s)	2639
MvaI	CCWGG	2 cut(s)	663, 1507
MvnI	CGCG	1 cut(s)	101
MwoI	GCNNNNNNNGC	8 cut(s)	215, 709, 1001, 1211, 1223, 1295, 2975, 3014
NaeI	GCCGGC	1 cut(s)	207
NciI	CCSGG	1 cut(s)	444
NcoI	CCATGG	2 cut(s)	82, 583
NdeI	CATATG	1 cut(s)	2740
NgoMIV	GCCGGC	1 cut(s)	205
NheI	GCTAGC	1 cut(s)	605
NlaIV	GGNNCC	9 cut(s)	133, 166, 189, 325, 561, 1003, 1782, 2590, 2984
NmuCI	GTSAC	5 cut(s)	1039, 1242, 1663, 2087, 2897
NsiI	ATGCAT	2 cut(s)	258, 405
NspI	RCATGY	6 cut(s)	2201, 2499, 2666, 2840, 2862, 2943
PaeI	GCATGC	1 cut(s)	2943
PagI	TCATGA	2 cut(s)	2110, 2604
PceI	AGGCCT	2 cut(s)	661, 1941
PciI	ACATGT	1 cut(s)	2495
PcsI	WCGNNNNNNNCGW	2 cut(s)	472, 1650
PctI	GAATGC	1 cut(s)	2639
PdiI	GCCGGC	1 cut(s)	207
PflMI	CCANNNNNTGG	2 cut(s)	320, 2908
PinAI	ACCGGT	1 cut(s)	3131
PkrI	GCNGC	9 cut(s)	6, 103, 969, 1216, 1347, 2077, 2176, 3007, 3180
PleI	GAGTC	3 cut(s)	224, 1330, 1921
PpsI	GAGTC	3 cut(s)	224, 1330, 1921
Ppu21I	YACGTR	2 cut(s)	467, 2533
PscI	ACATGT	1 cut(s)	2495
PshAI	GACNNNNGTC	1 cut(s)	2189
PshBI	ATTAAT	2 cut(s)	384, 1731
PsiI	TTATAA	2 cut(s)	1563, 3141
Psp6I	CCWGG	2 cut(s)	661, 1505
PspFI	CCCAGC	3 cut(s)	2517, 2521, 2978
PspGI	CCWGG	2 cut(s)	661, 1505
PspN4I	GGNNCC	9 cut(s)	133, 166, 189, 325, 561, 1003, 1782, 2590, 2984
PspPI	GGNCC	5 cut(s)	431, 1073, 1439, 2456, 3057
PstI	CTGCAG	1 cut(s)	2780
PstNI	CAGNNNCTG	5 cut(s)	202, 347, 1322, 1594, 2132
PsuI	RGATCY	6 cut(s)	131, 148, 1698, 1756, 2225, 2982
PvuII	CAGCTG	4 cut(s)	970, 2078, 2177, 2521
RsaI	GTAC	7 cut(s)	462, 1310, 1455, 1486, 1636, 1643, 2620
RsaNI	GTAC	7 cut(s)	461, 1309, 1454, 1485, 1635, 1642, 2619
RseI	CAYNNNNRTG	3 cut(s)	306, 2031, 2835
SatI	GCNGC	9 cut(s)	5, 102, 968, 1215, 1346, 2076, 2175, 3006, 3179
Sau96I	GGNCC	5 cut(s)	431, 1073, 1439, 2456, 3057
ScaI	AGTACT	1 cut(s)	1310
SchI	GAGTC	3 cut(s)	224, 1330, 1921
ScrFI	CCNGG	3 cut(s)	444, 663, 1507
SduI	GDGCHC	2 cut(s)	1278, 3197
SfcI	CTRYAG	2 cut(s)	2079, 2776
SinI	GGWCC	4 cut(s)	431, 1439, 2456, 3057
SmiMI	CAYNNNNRTG	3 cut(s)	306, 2031, 2835
SmlI	CTYRAG	4 cut(s)	554, 862, 1604, 2060
SmoI	CTYRAG	4 cut(s)	554, 862, 1604, 2060
SphI	GCATGC	1 cut(s)	2943
SseBI	AGGCCT	2 cut(s)	661, 1941
SsiI	CCGC	7 cut(s)	66, 101, 176, 701, 1804, 2807, 2834
SspI	AATATT	2 cut(s)	1069, 2338
SspMI	CTAG	7 cut(s)	246, 458, 534, 606, 818, 2150, 2408
StuI	AGGCCT	2 cut(s)	661, 1941
StyD4I	CCNGG	3 cut(s)	442, 661, 1505
StyI	CCWWGG	2 cut(s)	82, 583
TaaI	ACNGT	9 cut(s)	343, 568, 1661, 1852, 1969, 2210, 2784, 3114, 3121
TaiI	ACGT	6 cut(s)	196, 457, 469, 2535, 2720, 3165
TatI	WGTACW	1 cut(s)	1308
TauI	GCSGC	1 cut(s)	104
TscAI	CASTG	7 cut(s)	1201, 1305, 1347, 2044, 2599, 3067, 3117
TseFI	GTSAC	5 cut(s)	1039, 1242, 1663, 2087, 2897
TseI	GCWGC	8 cut(s)	4, 967, 1214, 1345, 2075, 2174, 3005, 3178
Tsp45I	GTSAC	5 cut(s)	1039, 1242, 1663, 2087, 2897
TspGWI	ACGGA	5 cut(s)	929, 973, 1551, 2358, 2654
TspRI	CASTG	7 cut(s)	1201, 1305, 1347, 2044, 2599, 3067, 3117
Van91I	CCANNNNNTGG	2 cut(s)	320, 2908
Vha464I	CTTAAG	1 cut(s)	2060
VpaK11BI	GGWCC	4 cut(s)	431, 1439, 2456, 3057
VspI	ATTAAT	2 cut(s)	384, 1731
XapI	RAATTY	6 cut(s)	211, 477, 509, 1423, 2819, 3077
XceI	RCATGY	6 cut(s)	2201, 2499, 2666, 2840, 2862, 2943
XmiI	GTMKAC	1 cut(s)	1140
XspI	CTAG	7 cut(s)	246, 458, 534, 606, 818, 2150, 2408
ZrmI	AGTACT	1 cut(s)	1310
Zsp2I	ATGCAT	2 cut(s)	258, 405

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.