RLG00000017921

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018781
Physical Location & Seq
Forward (+)
22963165 .. 22969395
6231 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017921

Sequence Viewer

Length: 1437 bp
ATGAATTCCCACAAGGTCTTGCGTCTTAATGGGAATGGCGCTGCATTGTTTCATGCTCCCGCCAAATCTTGGGATGGTGGATTTCTTGGCGTTTTGAACACCACCACCAGTGAATTCTTTGGGGACGAGGTTGAATTTGATTCCGACATCCTCAACTGGGATCCTATCCTCCATATTAGCTTCTGGGGTTTAACAATTCAAATGGGGATGCGTTTTGGATCAAGAACCAAACGGGCAGGAATCCCTTTTGCACCTTCCTCCATTAAGTTTCTATTTGGACAATCCTTAGATGAGATCAGCCTTGGGAGAAGATTGTTTAGATTAACCCCTTTCCAAAGGACCAAAAGAAAATCTCTCCATCCATGGAAGTACGATGTATTTTTGAGTTTCACTGGGGAAACCCGTAAGGGTTTCACTCACTTCCTGTACAAAGCCTTGCAAGGGCGAGGACTCGTTGTTTTCAGGGACCAAGAACTTGAAAGAGGCACAAACATTTCTTTGAAGCTCTTGACTGCAATAGAAGAATCGAGGTTTGCCATTGTTGTTCTCTCTCCAAAATATGCTTCTTCCACTTGGTGCTTGGACGAACTTTCAAAGATCTTTGAATGCATGGAAGTCCGTGGCACCATTCTTCCGATTTTTTACAAGGTGAAGCCCTCGAATATACGGAACCAAAGGGGAAGTTTTGCAAAAGCCTTCAACAAGCATGAATATCATAACATGGCAAAAGTGCAAAGGTGGAGGAGTGTTTTAACGAAAGTGGGCTGCATCGCTGGATGGATATCAAAAGATTGGTACGAGTCTGAGCTTATCGACGTAATTGTAGAATTAGTGTTGGCAAAATTGCTCGTGGTAATTTATTTTGTGTACGTAGGGAGCAGATTCTTCGGAATCACTTTCAAATGCACCAAAACACAACCATGGAAATACGATCTGTTTTTGAGTTTCACCGGGGAAACCCGCAAGGGTTTCACTCACTTCTTGTTCAAAGCATTGCAACGGCGAGGACTCAAAGTTTTCAGGGACCAAGAAATCCAAAGAGGAGCAGACATTTCTTCAATGCTCTCAACTGGAATTGAAGAATCGAGGTTTGCCATCATCATCCTTTCGCCAAAGTACGCTTCTTCTACTTGGTGCTTGGACGAACTTACTAAAATTGTGGAGTGCATGGAAGTCAAGGGCACAACAATTCTCCCAATATTTTATAACGTGAAGCCAACGAGTGTACGGAATCAAAGAGGAGCTTTTGCAAAAGCCTTCAAGAAGCATGATAATAATTATGTTAAAGATAAAGCAAAAGTGCAAAGGTGGAGAAGCGCTTTTACAAAACTGAGCGGTATCGCTGGATGGATATCAACAGAATGGTATGAGTACGAGCTGATTGAAGAAGTCGTGGAATTAGTGTGTGGCACATGCATCTTAGCTCGATCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

479

Amino Acids

54.98

Weight (kDa)

9.62

Isoelectric Point (pI)

42.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 123 - 282 2.1e-43 TIR domain
TIR_2 PF13676 126 - 220 6.4e-15 TIR domain
TIR PF01582 309 - 467 8.9e-45 TIR domain
TIR_2 PF13676 313 - 407 7.5e-16 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018781)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19921 FvH4_5g03280
rosa_laevigata RLG00000017921 RLG00000017922 RLG00000017924
rosa_multiflora Rmu_sc0000448.1_g000008
rosa_roxburghii Rroxscaffold_2G00132130
rosa_rugosa Rorug02G0176100
rosa_wichuraiana Rw2G017680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1206
AccB1I GGYRCC 1 cut(s) 623
AccB7I CCANNNNNTGG 1 cut(s) 69
AccBSI CCGCTC 1 cut(s) 1335
AciI CCGC 3 cut(s) 60, 961, 1335
AclWI GGATC 4 cut(s) 155, 168, 226, 1422
AcsI RAATTY 3 cut(s) 4, 113, 134
AdeI CACNNNGTG 1 cut(s) 576
AfaI GTAC 7 cut(s) 371, 428, 797, 869, 1118, 1227, 1373
AfeI AGCGCT 1 cut(s) 1318
AfiI CCNNNNNNNGG 3 cut(s) 69, 157, 441
AjuI GAANNNNNNNTTGG 4 cut(s) 547, 579, 1105, 1137
AluBI AGCT 6 cut(s) 180, 505, 808, 1244, 1378, 1424
AluI AGCT 6 cut(s) 180, 505, 808, 1244, 1378, 1424
AlwI GGATC 4 cut(s) 155, 168, 226, 1422
Aor51HI AGCGCT 1 cut(s) 1318
ApeKI GCWGC 2 cut(s) 41, 765
ApoI RAATTY 3 cut(s) 4, 113, 134
AspLEI GCGC 2 cut(s) 41, 1319
AspS9I GGNCC 3 cut(s) 339, 466, 1024
AsuC2I CCSGG 1 cut(s) 952
AsuHPI GGTGA 2 cut(s) 661, 940
AvaII GGWCC 3 cut(s) 339, 466, 1024
BaeGI GKGCMC 1 cut(s) 1184
BamHI GGATCC 1 cut(s) 160
BanI GGYRCC 1 cut(s) 623
BarI GAAGNNNNNNTAC 2 cut(s) 1306, 1338
BauI CACGAG 1 cut(s) 848
BbvI GCAGC 2 cut(s) 28, 752
BccI CCATC 5 cut(s) 68, 366, 771, 1103, 1341
BceAI ACGGC 1 cut(s) 1016
BcgI CGANNNNNNTGC 2 cut(s) 868, 902
BcnI CCSGG 1 cut(s) 952
BfoI RGCGCY 2 cut(s) 42, 1320
BglII AGATCT 1 cut(s) 597
BisI GCNGC 2 cut(s) 42, 766
BlsI GCNGC 2 cut(s) 43, 767
Bme1390I CCNGG 1 cut(s) 952
Bme18I GGWCC 3 cut(s) 339, 466, 1024
BmgT120I GGNCC 3 cut(s) 339, 466, 1024
BmiI GGNNCC 5 cut(s) 162, 467, 625, 671, 1025
BmrFI CCNGG 1 cut(s) 952
BmrI ACTGGG 2 cut(s) 166, 402
BmsI GCATC 3 cut(s) 198, 777, 1425
BmuI ACTGGG 2 cut(s) 166, 402
BpuMI CCSGG 1 cut(s) 952
BsaAI YACGTR 1 cut(s) 871
BsaBI GATNNNNATC 2 cut(s) 781, 1351
BsaJI CCNNGG 5 cut(s) 301, 362, 619, 920, 951
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 157, 441
Bse1I ACTGG 4 cut(s) 108, 161, 397, 1075
Bse3DI GCAATG 1 cut(s) 992
Bse8I GATNNNNATC 2 cut(s) 781, 1351
BseDI CCNNGG 5 cut(s) 301, 362, 619, 920, 951
BseGI GGATG 7 cut(s) 79, 147, 213, 358, 782, 1101, 1352
BseJI GATNNNNATC 2 cut(s) 781, 1351
BseLI CCNNNNNNNGG 3 cut(s) 69, 157, 441
BseMI GCAATG 1 cut(s) 992
BseMII CTCAG 2 cut(s) 795, 1322
BseNI ACTGG 4 cut(s) 108, 161, 397, 1075
BseRI GAGGAG 3 cut(s) 757, 1056, 1254
BseSI GKGCMC 1 cut(s) 1184
BseXI GCAGC 2 cut(s) 28, 752
BshNI GGYRCC 1 cut(s) 623
BsiSI CCGG 1 cut(s) 951
BslFI GGGAC 3 cut(s) 137, 479, 1037
BslI CCNNNNNNNGG 3 cut(s) 69, 157, 441
BsmFI GGGAC 3 cut(s) 137, 479, 1037
BsmI GAATGC 1 cut(s) 611
Bsp1286I GDGCHC 1 cut(s) 1184
Bsp1407I TGTACA 1 cut(s) 426
Bsp143I GATC 6 cut(s) 160, 218, 294, 597, 931, 1427
Bsp19I CCATGG 2 cut(s) 362, 920
BspACI CCGC 3 cut(s) 60, 961, 1335
BspCNI CTCAG 2 cut(s) 796, 1323
BspLI GGNNCC 5 cut(s) 162, 467, 625, 671, 1025
BspPI GGATC 4 cut(s) 155, 168, 226, 1422
BspT107I GGYRCC 1 cut(s) 623
BsrBI CCGCTC 1 cut(s) 1335
BsrDI GCAATG 1 cut(s) 992
BsrGI TGTACA 1 cut(s) 426
BsrI ACTGG 4 cut(s) 108, 161, 397, 1075
BssECI CCNNGG 5 cut(s) 301, 362, 619, 920, 951
BssMI GATC 6 cut(s) 160, 218, 294, 597, 931, 1427
BssSI CACGAG 1 cut(s) 848
BssT1I CCWWGG 3 cut(s) 301, 362, 920
Bst2BI CACGAG 1 cut(s) 848
BstAUI TGTACA 1 cut(s) 426
BstBAI YACGTR 1 cut(s) 871
BstDEI CTNAG 4 cut(s) 286, 804, 1331, 1420
BstDSI CCRYGG 3 cut(s) 362, 619, 920
BstF5I GGATG 7 cut(s) 79, 147, 213, 358, 782, 1101, 1352
BstH2I RGCGCY 2 cut(s) 42, 1320
BstHHI GCGC 2 cut(s) 41, 1319
BstKTI GATC 6 cut(s) 163, 221, 297, 600, 934, 1430
BstMBI GATC 6 cut(s) 160, 218, 294, 597, 931, 1427
BstNSI RCATGY 1 cut(s) 1416
BstSCI CCNGG 1 cut(s) 950
BstSLI GKGCMC 1 cut(s) 1184
BstSNI TACGTA 1 cut(s) 871
BstV1I GCAGC 2 cut(s) 28, 752
BstX2I RGATCY 2 cut(s) 160, 597
BstYI RGATCY 2 cut(s) 160, 597
BtgI CCRYGG 3 cut(s) 362, 619, 920
BtgZI GCGATG 1 cut(s) 754
BtsCI GGATG 7 cut(s) 79, 147, 213, 358, 782, 1101, 1352
BtsIMutI CAGTG 2 cut(s) 115, 390
CfoI GCGC 2 cut(s) 41, 1319
Cfr13I GGNCC 3 cut(s) 339, 466, 1024
CseI GACGC 1 cut(s) 11
Csp6I GTAC 7 cut(s) 370, 427, 796, 868, 1117, 1226, 1372
CviAII CATG 9 cut(s) 53, 363, 610, 707, 721, 921, 1168, 1268, 1413
CviQI GTAC 7 cut(s) 370, 427, 796, 868, 1117, 1226, 1372
DdeI CTNAG 4 cut(s) 286, 804, 1331, 1420
DpnI GATC 6 cut(s) 162, 220, 296, 599, 933, 1429
DpnII GATC 6 cut(s) 160, 218, 294, 597, 931, 1427
DraIII CACNNNGTG 1 cut(s) 576
Eco105I TACGTA 1 cut(s) 871
Eco130I CCWWGG 3 cut(s) 301, 362, 920
Eco32I GATATC 2 cut(s) 783, 1353
Eco47I GGWCC 3 cut(s) 339, 466, 1024
Eco47III AGCGCT 1 cut(s) 1318
EcoRI GAATTC 2 cut(s) 4, 113
EcoRV GATATC 2 cut(s) 783, 1353
EcoT14I CCWWGG 3 cut(s) 301, 362, 920
EcoT22I ATGCAT 2 cut(s) 611, 1418
ErhI CCWWGG 3 cut(s) 301, 362, 920
FaeI CATG 9 cut(s) 56, 366, 613, 710, 724, 924, 1171, 1271, 1416
FalI AAGNNNNNCTT 2 cut(s) 1228, 1260
FaqI GGGAC 3 cut(s) 137, 479, 1037
FatI CATG 9 cut(s) 52, 362, 609, 706, 720, 920, 1167, 1267, 1412
FauI CCCGC 2 cut(s) 67, 968
Fnu4HI GCNGC 2 cut(s) 42, 766
FokI GGATG 7 cut(s) 86, 134, 220, 345, 789, 1088, 1359
Fsp4HI GCNGC 2 cut(s) 42, 766
GlaI GCGC 2 cut(s) 40, 1318
GluI GCNGC 2 cut(s) 42, 766
HaeII RGCGCY 2 cut(s) 42, 1320
HapII CCGG 1 cut(s) 951
HgaI GACGC 1 cut(s) 11
HhaI GCGC 2 cut(s) 41, 1319
Hin1II CATG 9 cut(s) 56, 366, 613, 710, 724, 924, 1171, 1271, 1416
Hin6I GCGC 2 cut(s) 39, 1317
HinP1I GCGC 2 cut(s) 39, 1317
HpaII CCGG 1 cut(s) 951
HphI GGTGA 2 cut(s) 661, 940
Hpy166II GTNNAC 2 cut(s) 868, 1226
Hpy188I TCNGA 4 cut(s) 145, 636, 805, 890
Hpy188III TCNNGA 3 cut(s) 222, 508, 1261
Hpy8I GTNNAC 2 cut(s) 868, 1226
Hpy99I CGWCG 1 cut(s) 818
HpyAV CCTTC 3 cut(s) 264, 706, 1267
HpyCH4IV ACGT 3 cut(s) 816, 870, 1209
HpyF3I CTNAG 4 cut(s) 286, 804, 1331, 1420
HpySE526I ACGT 3 cut(s) 816, 870, 1209
Hsp92II CATG 9 cut(s) 56, 366, 613, 710, 724, 924, 1171, 1271, 1416
HspAI GCGC 2 cut(s) 39, 1317
Kzo9I GATC 6 cut(s) 160, 218, 294, 597, 931, 1427
LmnI GCTCC 4 cut(s) 61, 876, 1043, 1241
Lsp1109I GCAGC 2 cut(s) 28, 752
LweI GCATC 3 cut(s) 198, 777, 1425
MaeII ACGT 3 cut(s) 816, 870, 1209
MalI GATC 6 cut(s) 162, 220, 296, 599, 933, 1429
MbiI CCGCTC 1 cut(s) 1335
MboI GATC 6 cut(s) 160, 218, 294, 597, 931, 1427
MboII GAAGA 9 cut(s) 321, 533, 558, 623, 877, 1047, 1091, 1116, 1397
MflI RGATCY 2 cut(s) 160, 597
MhlI GDGCHC 1 cut(s) 1184
MlyI GAGTC 3 cut(s) 444, 809, 1002
MmeI TCCRAC 1 cut(s) 168
Mph1103I ATGCAT 2 cut(s) 611, 1418
MseI TTAA 6 cut(s) 27, 191, 264, 323, 752, 1284
MslI CAYNNNNRTG 1 cut(s) 919
MspI CCGG 1 cut(s) 951
MspR9I CCNGG 1 cut(s) 952
Mva1269I GAATGC 1 cut(s) 611
NciI CCSGG 1 cut(s) 952
NcoI CCATGG 2 cut(s) 362, 920
NdeII GATC 6 cut(s) 160, 218, 294, 597, 931, 1427
NlaIII CATG 9 cut(s) 56, 366, 613, 710, 724, 924, 1171, 1271, 1416
NlaIV GGNNCC 5 cut(s) 162, 467, 625, 671, 1025
NsiI ATGCAT 2 cut(s) 611, 1418
NspI RCATGY 1 cut(s) 1416
PctI GAATGC 1 cut(s) 611
PfeI GAWTC 7 cut(s) 140, 240, 524, 882, 891, 1082, 1231
PflMI CCANNNNNTGG 1 cut(s) 69
PkrI GCNGC 2 cut(s) 43, 767
PleI GAGTC 3 cut(s) 444, 808, 1002
PpsI GAGTC 3 cut(s) 444, 808, 1002
Ppu21I YACGTR 1 cut(s) 871
PsiI TTATAA 1 cut(s) 1206
PspN4I GGNNCC 5 cut(s) 162, 467, 625, 671, 1025
PspPI GGNCC 3 cut(s) 339, 466, 1024
PsuI RGATCY 2 cut(s) 160, 597
RsaI GTAC 7 cut(s) 371, 428, 797, 869, 1118, 1227, 1373
RsaNI GTAC 7 cut(s) 370, 427, 796, 868, 1117, 1226, 1372
RseI CAYNNNNRTG 1 cut(s) 919
SaqAI TTAA 6 cut(s) 27, 191, 264, 323, 752, 1284
SatI GCNGC 2 cut(s) 42, 766
Sau3AI GATC 6 cut(s) 160, 218, 294, 597, 931, 1427
Sau96I GGNCC 3 cut(s) 339, 466, 1024
SchI GAGTC 3 cut(s) 444, 809, 1002
ScrFI CCNGG 1 cut(s) 952
SduI GDGCHC 1 cut(s) 1184
SfaNI GCATC 3 cut(s) 198, 777, 1425
SinI GGWCC 3 cut(s) 339, 466, 1024
SmiMI CAYNNNNRTG 1 cut(s) 919
SnaBI TACGTA 1 cut(s) 871
SsiI CCGC 3 cut(s) 60, 961, 1335
SspI AATATT 1 cut(s) 1200
StyD4I CCNGG 1 cut(s) 950
StyI CCWWGG 3 cut(s) 301, 362, 920
TaiI ACGT 3 cut(s) 819, 873, 1212
TaqI TCGA 5 cut(s) 527, 659, 813, 1085, 1426
TatI WGTACW 1 cut(s) 426
TfiI GAWTC 7 cut(s) 140, 240, 524, 882, 891, 1082, 1231
Tru1I TTAA 6 cut(s) 27, 191, 264, 323, 752, 1284
Tru9I TTAA 6 cut(s) 27, 191, 264, 323, 752, 1284
TscAI CASTG 2 cut(s) 115, 397
TseI GCWGC 2 cut(s) 41, 765
TspDTI ATGAA 3 cut(s) 17, 41, 723
TspGWI ACGGA 3 cut(s) 608, 682, 1243
TspRI CASTG 2 cut(s) 115, 397
Van91I CCANNNNNTGG 1 cut(s) 69
VpaK11BI GGWCC 3 cut(s) 339, 466, 1024
XapI RAATTY 3 cut(s) 4, 113, 134
XceI RCATGY 1 cut(s) 1416
XcmI CCANNNNNNNNNTGG 1 cut(s) 577
Zsp2I ATGCAT 2 cut(s) 611, 1418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.