RLG00000017924

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018781
Physical Location & Seq
Forward (+)
23024603 .. 23025445
843 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017924

Sequence Viewer

Length: 627 bp
ATGGAAGCAAATATCAAGGAACACATATACAAAGACAATATCAATGCAGCTAGGGCTATAACTCCACAGGCTTGTTTCATTTACTGGGCAGAAACGCTCGTATTTATTAATTGTATGTACGTCGCACTCTCATTGCTTGAAACCACTTTCAAATGGACCCGAGGATCAACTCTTTCAGCTGTTCTTCATCCCTGGGAATATGATGTCTTTTTGAGTTTCGCAGGAGAAACCCGCAAGGGTTTCACAGACCACTTGTACCGTGCATTGCAGAGGCGAGGAGTCAATGTTTTCAGGGACCAACAACTTGAAAGAGGTACAACTCTTTCATCAGAGCTCTTGACTGTGATCGAGGAGTCGAGGTTTGCAATCATCGTCCTCTCGCAAAATTATGCTTCCTCCACTTGGTGTTTAGATGAACTTTCAAAAGTTTTTGAATGCATGGAAGTCAGGGGCACAATACTGCCCATTTTTTATAAGGTGAAGCCCTCCAATGTTCGAAGACAAAGGGGAGCTTTTGCAAGAGCCTTCTACAAGCATGAATACAATAACATAGCAAAAGTGCAAAGATGGAGAAGTGCTTTCACAAGAGTGGGCGGGATCGCTGGATGGCTATCAGACGATTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

209

Amino Acids

24.17

Weight (kDa)

9.06

Isoelectric Point (pI)

49.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 67 - 208 1.1e-41 TIR domain
TIR_2 PF13676 69 - 163 3.5e-17 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018781)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19921 FvH4_5g03280
rosa_laevigata RLG00000017921 RLG00000017922 RLG00000017924
rosa_multiflora Rmu_sc0000448.1_g000008
rosa_roxburghii Rroxscaffold_2G00132130
rosa_rugosa Rorug02G0176100
rosa_wichuraiana Rw2G017680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 474
AciI CCGC 2 cut(s) 232, 594
AclWI GGATC 2 cut(s) 172, 605
AdeI CACNNNGTG 1 cut(s) 405
AfaI GTAC 3 cut(s) 119, 257, 316
AfiI CCNNNNNNNGG 1 cut(s) 402
AgsI TTSAA 5 cut(s) 140, 151, 308, 423, 434
AjnI CCWGG 1 cut(s) 191
AleI CACNNNNGTG 1 cut(s) 587
AluBI AGCT 4 cut(s) 50, 179, 334, 512
AluI AGCT 4 cut(s) 50, 179, 334, 512
Alw21I GWGCWC 1 cut(s) 336
AlwI GGATC 2 cut(s) 172, 605
Ama87I CYCGRG 1 cut(s) 159
ApeKI GCWGC 1 cut(s) 47
AseI ATTAAT 1 cut(s) 108
AspS9I GGNCC 2 cut(s) 156, 295
AsuHPI GGTGA 1 cut(s) 490
AsuII TTCGAA 1 cut(s) 496
AvaI CYCGRG 1 cut(s) 159
AvaII GGWCC 2 cut(s) 156, 295
BaeGI GKGCMC 1 cut(s) 455
BanII GRGCYC 1 cut(s) 336
BbsI GAAGAC 1 cut(s) 505
Bbv12I GWGCWC 1 cut(s) 336
BbvI GCAGC 1 cut(s) 59
BccI CCATC 2 cut(s) 561, 600
BciT130I CCWGG 1 cut(s) 193
BfaI CTAG 1 cut(s) 51
BisI GCNGC 1 cut(s) 48
BlsI GCNGC 1 cut(s) 49
Bme1390I CCNGG 1 cut(s) 193
Bme18I GGWCC 2 cut(s) 156, 295
BmeT110I CYCGRG 1 cut(s) 159
BmgT120I GGNCC 2 cut(s) 156, 295
BmiI GGNNCC 2 cut(s) 158, 296
BmrFI CCNGG 1 cut(s) 193
BmrI ACTGGG 1 cut(s) 94
BmuI ACTGGG 1 cut(s) 94
BpiI GAAGAC 1 cut(s) 505
Bpu14I TTCGAA 1 cut(s) 496
BsaBI GATNNNNATC 1 cut(s) 610
BsaJI CCNNGG 3 cut(s) 160, 191, 192
BsaXI ACNNNNNCTCC 4 cut(s) 270, 300, 344, 374
Bsc4I CCNNNNNNNGG 1 cut(s) 402
Bse1I ACTGG 1 cut(s) 89
Bse3DI GCAATG 2 cut(s) 131, 263
Bse8I GATNNNNATC 1 cut(s) 610
BseBI CCWGG 1 cut(s) 193
BseDI CCNNGG 3 cut(s) 160, 191, 192
BseGI GGATG 2 cut(s) 187, 611
BseJI GATNNNNATC 1 cut(s) 610
BseLI CCNNNNNNNGG 1 cut(s) 402
BseMI GCAATG 2 cut(s) 131, 263
BseNI ACTGG 1 cut(s) 89
BseRI GAGGAG 2 cut(s) 291, 365
BseSI GKGCMC 1 cut(s) 455
BseXI GCAGC 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 336
BsiHKCI CYCGRG 1 cut(s) 159
BslFI GGGAC 1 cut(s) 308
BslI CCNNNNNNNGG 1 cut(s) 402
BsmFI GGGAC 1 cut(s) 308
BsmI GAATGC 1 cut(s) 440
BsoBI CYCGRG 1 cut(s) 159
Bsp119I TTCGAA 1 cut(s) 496
Bsp1286I GDGCHC 2 cut(s) 336, 455
Bsp143I GATC 3 cut(s) 164, 345, 597
BspACI CCGC 2 cut(s) 232, 594
BspLI GGNNCC 2 cut(s) 158, 296
BspPI GGATC 2 cut(s) 172, 605
BspT104I TTCGAA 1 cut(s) 496
BsrDI GCAATG 2 cut(s) 131, 263
BsrI ACTGG 1 cut(s) 89
BssECI CCNNGG 3 cut(s) 160, 191, 192
BssMI GATC 3 cut(s) 164, 345, 597
Bst2UI CCWGG 1 cut(s) 193
Bst4CI ACNGT 2 cut(s) 260, 343
BstBI TTCGAA 1 cut(s) 496
BstF5I GGATG 2 cut(s) 187, 611
BstKTI GATC 3 cut(s) 167, 348, 600
BstMBI GATC 3 cut(s) 164, 345, 597
BstMWI GCNNNNNNNGC 1 cut(s) 53
BstNI CCWGG 1 cut(s) 193
BstSCI CCNGG 1 cut(s) 191
BstSLI GKGCMC 1 cut(s) 455
BstV1I GCAGC 1 cut(s) 59
BstV2I GAAGAC 1 cut(s) 505
BtsCI GGATG 2 cut(s) 187, 611
Cfr13I GGNCC 2 cut(s) 156, 295
Csp6I GTAC 3 cut(s) 118, 256, 315
CviAII CATG 2 cut(s) 439, 536
CviJI RGCY 9 cut(s) 50, 56, 71, 179, 334, 484, 512, 524, 610
CviKI_1 RGCY 9 cut(s) 50, 56, 71, 179, 334, 484, 512, 524, 610
CviQI GTAC 3 cut(s) 118, 256, 315
DpnI GATC 3 cut(s) 166, 347, 599
DpnII GATC 3 cut(s) 164, 345, 597
DraIII CACNNNGTG 1 cut(s) 405
Ecl136II GAGCTC 1 cut(s) 334
Eco24I GRGCYC 1 cut(s) 336
Eco47I GGWCC 2 cut(s) 156, 295
Eco53kI GAGCTC 1 cut(s) 334
Eco88I CYCGRG 1 cut(s) 159
EcoICRI GAGCTC 1 cut(s) 334
EcoRII CCWGG 1 cut(s) 191
EcoT22I ATGCAT 1 cut(s) 440
EcoT38I GRGCYC 1 cut(s) 336
FaeI CATG 2 cut(s) 442, 539
FalI AAGNNNNNCTT 2 cut(s) 496, 528
FaqI GGGAC 1 cut(s) 308
FatI CATG 2 cut(s) 438, 535
FauI CCCGC 2 cut(s) 239, 587
Fnu4HI GCNGC 1 cut(s) 48
FokI GGATG 2 cut(s) 174, 618
FriOI GRGCYC 1 cut(s) 336
Fsp4HI GCNGC 1 cut(s) 48
FspBI CTAG 1 cut(s) 51
GluI GCNGC 1 cut(s) 48
Hin1II CATG 2 cut(s) 442, 539
HinfI GANTC 2 cut(s) 279, 353
HphI GGTGA 1 cut(s) 490
Hpy188I TCNGA 2 cut(s) 331, 616
Hpy188III TCNNGA 1 cut(s) 337
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 1 cut(s) 535
HpyCH4III ACNGT 2 cut(s) 260, 343
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 7 cut(s) 47, 263, 268, 365, 438, 518, 562
HpyF10VI GCNNNNNNNGC 1 cut(s) 53
HpySE526I ACGT 1 cut(s) 120
Hsp92II CATG 2 cut(s) 442, 539
Kzo9I GATC 3 cut(s) 164, 345, 597
LmnI GCTCC 1 cut(s) 509
LpnPI CCDG 8 cut(s) 53, 70, 178, 205, 207, 277, 433, 588
Lsp1109I GCAGC 1 cut(s) 59
MaeI CTAG 1 cut(s) 51
MaeII ACGT 1 cut(s) 120
MalI GATC 3 cut(s) 166, 347, 599
MboI GATC 3 cut(s) 164, 345, 597
MboII GAAGA 2 cut(s) 176, 510
MhlI GDGCHC 2 cut(s) 336, 455
MluCI AATT 2 cut(s) 109, 385
MlyI GAGTC 2 cut(s) 288, 362
MnlI CCTC 9 cut(s) 155, 264, 269, 305, 343, 351, 386, 406, 496
Mph1103I ATGCAT 1 cut(s) 440
MseI TTAA 1 cut(s) 108
MslI CAYNNNNRTG 1 cut(s) 587
MspA1I CMGCKG 1 cut(s) 179
MspR9I CCNGG 1 cut(s) 193
Mva1269I GAATGC 1 cut(s) 440
MvaI CCWGG 1 cut(s) 193
MwoI GCNNNNNNNGC 1 cut(s) 53
NdeII GATC 3 cut(s) 164, 345, 597
NlaIII CATG 2 cut(s) 442, 539
NlaIV GGNNCC 2 cut(s) 158, 296
NsiI ATGCAT 1 cut(s) 440
NspV TTCGAA 1 cut(s) 496
OliI CACNNNNGTG 1 cut(s) 587
PasI CCCWGGG 1 cut(s) 192
PctI GAATGC 1 cut(s) 440
PkrI GCNGC 1 cut(s) 49
PleI GAGTC 2 cut(s) 287, 361
PpsI GAGTC 2 cut(s) 287, 361
PshBI ATTAAT 1 cut(s) 108
PsiI TTATAA 1 cut(s) 474
Psp124BI GAGCTC 1 cut(s) 336
Psp6I CCWGG 1 cut(s) 191
PspGI CCWGG 1 cut(s) 191
PspN4I GGNNCC 2 cut(s) 158, 296
PspPI GGNCC 2 cut(s) 156, 295
PvuII CAGCTG 1 cut(s) 179
RsaI GTAC 3 cut(s) 119, 257, 316
RsaNI GTAC 3 cut(s) 118, 256, 315
RseI CAYNNNNRTG 1 cut(s) 587
SacI GAGCTC 1 cut(s) 336
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 1 cut(s) 48
Sau3AI GATC 3 cut(s) 164, 345, 597
Sau96I GGNCC 2 cut(s) 156, 295
SchI GAGTC 2 cut(s) 288, 362
ScrFI CCNGG 1 cut(s) 193
SduI GDGCHC 2 cut(s) 336, 455
SetI ASST 8 cut(s) 52, 123, 181, 316, 336, 362, 480, 514
SfuI TTCGAA 1 cut(s) 496
SinI GGWCC 2 cut(s) 156, 295
SmiMI CAYNNNNRTG 1 cut(s) 587
Sse9I AATT 2 cut(s) 109, 385
SsiI CCGC 2 cut(s) 232, 594
SspMI CTAG 1 cut(s) 51
SstI GAGCTC 1 cut(s) 336
StyD4I CCNGG 1 cut(s) 191
TaaI ACNGT 2 cut(s) 260, 343
TaiI ACGT 1 cut(s) 123
TaqI TCGA 3 cut(s) 348, 356, 496
TasI AATT 2 cut(s) 109, 385
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TseI GCWGC 1 cut(s) 47
TspDTI ATGAA 5 cut(s) 67, 176, 315, 429, 552
VpaK11BI GGWCC 2 cut(s) 156, 295
VspI ATTAAT 1 cut(s) 108
XspI CTAG 1 cut(s) 51
Zsp2I ATGCAT 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.