RLG00000017922

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018781
Physical Location & Seq
Forward (+)
22975930 .. 22985273
9344 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017922

Sequence Viewer

Length: 1245 bp
ATGCGGAGGCTGTGCAGCAGCTGTGCGGAGGCTGTGCAGGTGCTGTCGGCAGATGTCGCAGGGGTGTCGGCAGGCGTCGGCAGCTATCGGCAGGTGTGTGCAGGGCTGTCGGCAGCTTCTCGGCAGGGGCCTCGGCAGGTGTCGGCAGGTGATAGGCAGCTATCGGCAGGTGCTCGGCAGGTGTCGACAGGTCTCGGCAGGTGCTCTGCAGGTCTCGGCAGGTGCTCGGCAGGTGTCGGCAGGTGCTCGGCAGGTCTCGGCAGGTGTCGGCAGGTGCTCGGCAGGTGTGGCAGGTGCTCGGCAGGTCTCGGCAGATTGTTCGGAACCACTTTCAAATGCACCAAAACACAACCATGGAAGTACGATGTGTTTTTGAGTTTCACTGGGGAAACCCGCAAGGGTTTCACTCACTTCTTGTACAAAGCCTTGCAAGGTCGAGGACTCTGTGTTTTCAGGGACCAAGAACTTGAAAGAGGAGCAAGTGAGTTTGAAGTTCAAGCGTCAATCAATCGACAGTTCCAGATAGAATCGTGGGCTGCATCGCTGGATGGATATCAAAAGATTGATATGAGTCTGAGCTTATCGATACAGAGCAAAAGTGCAGAGTTGGAGAAGCACTTTTACAAAACTGAGCGGTATCGCTGGATGGATATCTACAGAATGCCAGCCATCCGAATCACTGTCAAATGGACTCGAGGAGTCTCCATATTAGCTCCTGCAACTGATTTTCAATCGCGGAAATATGATGTCTTTCTGAGCTTCACTGGAGAAACGAGAAAAAATTTCACAGACCACTTGTATAAAGAATTGCAGAGGGGAGGAGTCCAAGTCTTTAGAGACAAACAACTTGAACGAGGAACAGCTCTTTCTTCAAAGCTCTTGACTGCAATCGAGGACTCGGAGTTTGCCATTGTTGTCATCTCACCGCATTACGCGTCTTCATCTTGGTGTATGAGTGAGCTTTCGACCATTTTCGAATCCAAAGTCAGTGGCACAATTCTCCCTGTTTTCTATCGGGTCAAGCCCTCCGACATAAGATGTGCAAGGGGTAGTTTTGCGAGAGCCTTTAGGAAGCATGAAAATCGTAACATAGCAAACGTGCAAGAATGGAGAAGTGCTTTTACAAAATTGGGCGGCATCACTGGGTGGGTCTCAAAAGATTGGTACGAGTCTGAGCTCATAGAAGATATTGTACAATTTGTACGTAACGCAAAATCTGCACATACTTATTCTGAGACTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

415

Amino Acids

46.18

Weight (kDa)

9.59

Isoelectric Point (pI)

38.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 247 - 411 2.2e-40 TIR domain
TIR_2 PF13676 250 - 343 6.6e-17 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018781)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19921 FvH4_5g03280
rosa_laevigata RLG00000017921 RLG00000017922 RLG00000017924
rosa_multiflora Rmu_sc0000448.1_g000008
rosa_roxburghii Rroxscaffold_2G00132130
rosa_rugosa Rorug02G0176100
rosa_wichuraiana Rw2G017680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 634
AccI GTMKAC 1 cut(s) 185
AccII CGCG 2 cut(s) 736, 935
AciI CCGC 7 cut(s) 4, 26, 394, 634, 736, 926, 1134
AcsI RAATTY 1 cut(s) 781
AcyI GRCGYC 1 cut(s) 75
AdeI CACNNNGTG 1 cut(s) 1146
AfaI GTAC 5 cut(s) 362, 419, 1166, 1194, 1203
AflIII ACRYGT 1 cut(s) 933
AgsI TTSAA 7 cut(s) 334, 470, 491, 497, 731, 851, 873
Alw21I GWGCWC 7 cut(s) 175, 206, 227, 248, 279, 299, 1179
Alw26I GTCTC 8 cut(s) 197, 218, 260, 311, 706, 831, 1156, 1229
AlwNI CAGNNNCTG 2 cut(s) 21, 43
Ama87I CYCGRG 1 cut(s) 693
AoxI GGCC 1 cut(s) 128
ApeKI GCWGC 6 cut(s) 15, 18, 81, 113, 157, 536
ApoI RAATTY 1 cut(s) 781
ArsI GACNNNNNNTTYG 4 cut(s) 887, 919, 969, 1001
AspS9I GGNCC 2 cut(s) 128, 457
AsuHPI GGTGA 2 cut(s) 161, 915
AsuII TTCGAA 1 cut(s) 975
AvaI CYCGRG 1 cut(s) 693
AvaII GGWCC 1 cut(s) 457
BanII GRGCYC 1 cut(s) 1179
BarI GAAGNNNNNNTAC 6 cut(s) 605, 637, 1105, 1137, 1176, 1208
BbsI GAAGAC 1 cut(s) 930
Bbv12I GWGCWC 7 cut(s) 175, 206, 227, 248, 279, 299, 1179
BbvI GCAGC 6 cut(s) 27, 30, 93, 125, 169, 523
BccI CCATC 3 cut(s) 542, 640, 677
BcoDI GTCTC 8 cut(s) 197, 218, 260, 311, 706, 831, 1156, 1229
BfmI CTRYAG 2 cut(s) 207, 655
BisI GCNGC 7 cut(s) 16, 19, 82, 114, 158, 537, 1135
BlsI GCNGC 7 cut(s) 17, 20, 83, 115, 159, 538, 1136
Bme18I GGWCC 1 cut(s) 457
BmeT110I CYCGRG 1 cut(s) 693
BmgT120I GGNCC 2 cut(s) 128, 457
BmiI GGNNCC 3 cut(s) 129, 325, 458
BmrI ACTGGG 2 cut(s) 393, 1152
BmsI GCATC 2 cut(s) 548, 1146
BmuI ACTGGG 2 cut(s) 393, 1152
BpiI GAAGAC 1 cut(s) 930
BpmI CTGGAG 1 cut(s) 786
Bpu14I TTCGAA 1 cut(s) 975
Bsa29I ATCGAT 1 cut(s) 584
BsaAI YACGTR 1 cut(s) 1205
BsaBI GATNNNNATC 2 cut(s) 552, 650
BsaHI GRCGYC 1 cut(s) 75
BsaI GGTCTC 5 cut(s) 197, 218, 260, 311, 1156
BsaJI CCNNGG 2 cut(s) 131, 353
BsaXI ACNNNNNCTCC 2 cut(s) 813, 843
Bse1I ACTGG 3 cut(s) 388, 769, 1147
Bse8I GATNNNNATC 2 cut(s) 552, 650
BseCI ATCGAT 1 cut(s) 584
BseDI CCNNGG 2 cut(s) 131, 353
BseGI GGATG 3 cut(s) 553, 651, 669
BseJI GATNNNNATC 2 cut(s) 552, 650
BseMII CTCAG 5 cut(s) 566, 621, 746, 1164, 1224
BseNI ACTGG 3 cut(s) 388, 769, 1147
BseRI GAGGAG 3 cut(s) 489, 711, 834
BseXI GCAGC 6 cut(s) 27, 30, 93, 125, 169, 523
BsgI GTGCAG 5 cut(s) 34, 56, 120, 621, 1203
Bsh1236I CGCG 2 cut(s) 736, 935
BshFI GGCC 1 cut(s) 130
BshVI ATCGAT 1 cut(s) 584
BsiHKAI GWGCWC 7 cut(s) 175, 206, 227, 248, 279, 299, 1179
BsiHKCI CYCGRG 1 cut(s) 693
BslFI GGGAC 1 cut(s) 470
BsmAI GTCTC 8 cut(s) 197, 218, 260, 311, 706, 831, 1156, 1229
BsmFI GGGAC 1 cut(s) 470
BsmI GAATGC 1 cut(s) 666
BsnI GGCC 1 cut(s) 130
Bso31I GGTCTC 5 cut(s) 197, 218, 260, 311, 1156
BsoBI CYCGRG 1 cut(s) 693
Bsp119I TTCGAA 1 cut(s) 975
Bsp1286I GDGCHC 7 cut(s) 175, 206, 227, 248, 279, 299, 1179
Bsp1407I TGTACA 2 cut(s) 417, 1192
Bsp19I CCATGG 1 cut(s) 353
BspACI CCGC 7 cut(s) 4, 26, 394, 634, 736, 926, 1134
BspANI GGCC 1 cut(s) 130
BspCNI CTCAG 5 cut(s) 567, 622, 747, 1165, 1225
BspDI ATCGAT 1 cut(s) 584
BspFNI CGCG 2 cut(s) 736, 935
BspLI GGNNCC 3 cut(s) 129, 325, 458
BspMAI CTGCAG 1 cut(s) 211
BspT104I TTCGAA 1 cut(s) 975
BspTNI GGTCTC 5 cut(s) 197, 218, 260, 311, 1156
BsrBI CCGCTC 1 cut(s) 634
BsrGI TGTACA 2 cut(s) 417, 1192
BsrI ACTGG 3 cut(s) 388, 769, 1147
BssECI CCNNGG 2 cut(s) 131, 353
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 1 cut(s) 353
Bst4CI ACNGT 2 cut(s) 516, 682
BstACI GRCGYC 1 cut(s) 75
BstAPI GCANNNNNTGC 1 cut(s) 1217
BstAUI TGTACA 2 cut(s) 417, 1192
BstBAI YACGTR 1 cut(s) 1205
BstBI TTCGAA 1 cut(s) 975
BstC8I GCNNGC 2 cut(s) 73, 666
BstDEI CTNAG 5 cut(s) 575, 630, 755, 1173, 1233
BstDSI CCRYGG 1 cut(s) 353
BstF5I GGATG 3 cut(s) 553, 651, 669
BstFNI CGCG 2 cut(s) 736, 935
BstMAI GTCTC 8 cut(s) 197, 218, 260, 311, 706, 831, 1156, 1229
BstMWI GCNNNNNNNGC 4 cut(s) 56, 81, 288, 1217
BstSFI CTRYAG 2 cut(s) 207, 655
BstSNI TACGTA 1 cut(s) 1205
BstUI CGCG 2 cut(s) 736, 935
BstV1I GCAGC 6 cut(s) 27, 30, 93, 125, 169, 523
BstV2I GAAGAC 1 cut(s) 930
Bsu15I ATCGAT 1 cut(s) 584
BsuRI GGCC 1 cut(s) 130
BsuTUI ATCGAT 1 cut(s) 584
BtgI CCRYGG 1 cut(s) 353
BtgZI GCGATG 1 cut(s) 525
BtsCI GGATG 3 cut(s) 553, 651, 669
BtsIMutI CAGTG 5 cut(s) 381, 678, 762, 994, 1140
Cac8I GCNNGC 2 cut(s) 73, 666
CaiI CAGNNNCTG 2 cut(s) 21, 43
Cfr13I GGNCC 2 cut(s) 128, 457
ClaI ATCGAT 1 cut(s) 584
CseI GACGC 3 cut(s) 64, 489, 924
Csp6I GTAC 5 cut(s) 361, 418, 1165, 1193, 1202
CspCI CAANNNNNGTGG 2 cut(s) 970, 1005
CviAII CATG 2 cut(s) 354, 1076
CviQI GTAC 5 cut(s) 361, 418, 1165, 1193, 1202
DdeI CTNAG 5 cut(s) 575, 630, 755, 1173, 1233
DraIII CACNNNGTG 1 cut(s) 1146
Ecl136II GAGCTC 1 cut(s) 1177
Eco105I TACGTA 1 cut(s) 1205
Eco130I CCWWGG 1 cut(s) 353
Eco24I GRGCYC 1 cut(s) 1179
Eco31I GGTCTC 5 cut(s) 197, 218, 260, 311, 1156
Eco32I GATATC 2 cut(s) 554, 652
Eco47I GGWCC 1 cut(s) 457
Eco53kI GAGCTC 1 cut(s) 1177
Eco88I CYCGRG 1 cut(s) 693
EcoICRI GAGCTC 1 cut(s) 1177
EcoO109I RGGNCCY 1 cut(s) 128
EcoRV GATATC 2 cut(s) 554, 652
EcoT14I CCWWGG 1 cut(s) 353
EcoT38I GRGCYC 1 cut(s) 1179
ErhI CCWWGG 1 cut(s) 353
FaeI CATG 2 cut(s) 357, 1079
FaqI GGGAC 1 cut(s) 470
FatI CATG 2 cut(s) 353, 1075
FauI CCCGC 1 cut(s) 401
FblI GTMKAC 1 cut(s) 185
Fnu4HI GCNGC 7 cut(s) 16, 19, 82, 114, 158, 537, 1135
FokI GGATG 3 cut(s) 560, 656, 658
FriOI GRGCYC 1 cut(s) 1179
Fsp4HI GCNGC 7 cut(s) 16, 19, 82, 114, 158, 537, 1135
GluI GCNGC 7 cut(s) 16, 19, 82, 114, 158, 537, 1135
GsuI CTGGAG 1 cut(s) 786
HaeIII GGCC 1 cut(s) 130
HgaI GACGC 3 cut(s) 64, 489, 924
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 2 cut(s) 357, 1079
HincII GTYRAC 1 cut(s) 186
HindII GTYRAC 1 cut(s) 186
HphI GGTGA 2 cut(s) 161, 915
Hpy166II GTNNAC 1 cut(s) 186
Hpy188I TCNGA 8 cut(s) 323, 576, 674, 756, 901, 1030, 1174, 1234
Hpy188III TCNNGA 2 cut(s) 520, 880
Hpy8I GTNNAC 1 cut(s) 186
Hpy99I CGWCG 1 cut(s) 80
HpyCH4III ACNGT 2 cut(s) 516, 682
HpyCH4IV ACGT 2 cut(s) 1098, 1204
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 81, 288, 1217
HpyF3I CTNAG 5 cut(s) 575, 630, 755, 1173, 1233
HpySE526I ACGT 2 cut(s) 1098, 1204
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 2 cut(s) 357, 1079
LmnI GCTCC 2 cut(s) 476, 718
Lsp1109I GCAGC 6 cut(s) 27, 30, 93, 125, 169, 523
LweI GCATC 2 cut(s) 548, 1146
MaeII ACGT 2 cut(s) 1098, 1204
MaeIII GTNAC 2 cut(s) 1085, 1205
MbiI CCGCTC 1 cut(s) 634
MboII GAAGA 3 cut(s) 861, 930, 1196
MhlI GDGCHC 7 cut(s) 175, 206, 227, 248, 279, 299, 1179
MluCI AATT 6 cut(s) 781, 806, 996, 1127, 1196, 1240
MluI ACGCGT 1 cut(s) 933
MlyI GAGTC 7 cut(s) 435, 580, 685, 708, 831, 890, 1178
MmeI TCCRAC 2 cut(s) 588, 1053
MslI CAYNNNNRTG 2 cut(s) 352, 946
MspA1I CMGCKG 1 cut(s) 21
Mva1269I GAATGC 1 cut(s) 666
MvnI CGCG 2 cut(s) 736, 935
MwoI GCNNNNNNNGC 4 cut(s) 56, 81, 288, 1217
NcoI CCATGG 1 cut(s) 353
NlaIII CATG 2 cut(s) 357, 1079
NlaIV GGNNCC 3 cut(s) 129, 325, 458
NspV TTCGAA 1 cut(s) 975
PaeR7I CTCGAG 1 cut(s) 693
PctI GAATGC 1 cut(s) 666
PfeI GAWTC 3 cut(s) 527, 675, 977
PkrI GCNGC 7 cut(s) 17, 20, 83, 115, 159, 538, 1136
PleI GAGTC 7 cut(s) 435, 579, 685, 707, 830, 890, 1177
PpsI GAGTC 7 cut(s) 435, 579, 685, 707, 830, 890, 1177
Ppu21I YACGTR 1 cut(s) 1205
Psp124BI GAGCTC 1 cut(s) 1179
PspN4I GGNNCC 3 cut(s) 129, 325, 458
PspPI GGNCC 2 cut(s) 128, 457
PspXI VCTCGAGB 1 cut(s) 693
PstI CTGCAG 1 cut(s) 211
PstNI CAGNNNCTG 2 cut(s) 21, 43
PvuII CAGCTG 1 cut(s) 21
RsaI GTAC 5 cut(s) 362, 419, 1166, 1194, 1203
RsaNI GTAC 5 cut(s) 361, 418, 1165, 1193, 1202
RseI CAYNNNNRTG 2 cut(s) 352, 946
SacI GAGCTC 1 cut(s) 1179
SalI GTCGAC 1 cut(s) 184
SatI GCNGC 7 cut(s) 16, 19, 82, 114, 158, 537, 1135
Sau96I GGNCC 2 cut(s) 128, 457
SchI GAGTC 7 cut(s) 435, 580, 685, 708, 831, 890, 1178
SduI GDGCHC 7 cut(s) 175, 206, 227, 248, 279, 299, 1179
SfaNI GCATC 2 cut(s) 548, 1146
SfcI CTRYAG 2 cut(s) 207, 655
Sfr274I CTCGAG 1 cut(s) 693
SfuI TTCGAA 1 cut(s) 975
SinI GGWCC 1 cut(s) 457
SlaI CTCGAG 1 cut(s) 693
SmiMI CAYNNNNRTG 2 cut(s) 352, 946
SmlI CTYRAG 1 cut(s) 693
SmoI CTYRAG 1 cut(s) 693
SnaBI TACGTA 1 cut(s) 1205
Sse9I AATT 6 cut(s) 781, 806, 996, 1127, 1196, 1240
SsiI CCGC 7 cut(s) 4, 26, 394, 634, 736, 926, 1134
SstI GAGCTC 1 cut(s) 1179
StyI CCWWGG 1 cut(s) 353
TaaI ACNGT 2 cut(s) 516, 682
TaiI ACGT 2 cut(s) 1101, 1207
TaqI TCGA 8 cut(s) 185, 436, 511, 584, 694, 891, 965, 975
TasI AATT 6 cut(s) 781, 806, 996, 1127, 1196, 1240
TatI WGTACW 2 cut(s) 417, 1192
TauI GCSGC 1 cut(s) 1137
TfiI GAWTC 3 cut(s) 527, 675, 977
TscAI CASTG 5 cut(s) 388, 685, 769, 994, 1147
TseI GCWGC 6 cut(s) 15, 18, 81, 113, 157, 536
TspDTI ATGAA 2 cut(s) 930, 1092
TspRI CASTG 5 cut(s) 388, 685, 769, 994, 1147
VpaK11BI GGWCC 1 cut(s) 457
XapI RAATTY 1 cut(s) 781
XhoI CTCGAG 1 cut(s) 693
XmiI GTMKAC 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.