Rroxscaffold_2G00132130

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0018781
Physical Location & Seq
Reverse (-)
69151106 .. 69154832
3727 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00132130.1

Sequence Viewer

Length: 561 bp
ATGAATACAGCGAGTGAGAAGAGGTCCCGCCGCGGGTTTATATCATTTGTGGGGAGCAGATTGTTCGGAATCACTTTCAAATGCACCAAAACACAACCATGGAAATACGATGTGTTTTTGAGTTTCACCGGGGAAACCCGCAAGGGTTTCACTCACTTCTTGTACAAAGCATTGCAACGGCGAGGACTCAAAGTTTTCAGGGACCAAGAAATCCAAAGAGGTGCAAACATTTCTTCAATGCTCTCAACCGGAATTGAAGAATCGAGGTTTGCCATCATCATCCTTTCGCCAAAGTACGCTTCTTCTACTTGGTGCTTGGACGAACTTACTAAAATTGTGGAGTGCATGGAAGTCAAGGGCACAACAATTCTCCCAATATTTTATAAGGTGAAGCCAACGAGTGTAAGGAATCAAAGAGGAGCTTTTGCAAAAGCCTTCAAGAAGCATGATAATAATTATGTTAAAGATAACGCAAAAGTGCAAAGGTGGAGAAGCGCTTTTACAAAACTGAGCGGTATCGCTGGATGGATATCAACAGAATGGTACGCAGAGTCTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

186

Amino Acids

21.41

Weight (kDa)

10.08

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 35 - 185 1.6e-46 TIR domain
TIR_2 PF13676 38 - 133 2.9e-18 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018781)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19921 FvH4_5g03280
rosa_laevigata RLG00000017921 RLG00000017922 RLG00000017924
rosa_multiflora Rmu_sc0000448.1_g000008
rosa_roxburghii Rroxscaffold_2G00132130
rosa_rugosa Rorug02G0176100
rosa_wichuraiana Rw2G017680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 384
AccBSI CCGCTC 1 cut(s) 513
AccII CGCG 1 cut(s) 33
AciI CCGC 5 cut(s) 28, 31, 33, 139, 513
AfaI GTAC 3 cut(s) 164, 296, 545
AfeI AGCGCT 1 cut(s) 496
AfiI CCNNNNNNNGG 1 cut(s) 33
AgsI TTSAA 4 cut(s) 79, 237, 257, 439
AjuI GAANNNNNNNTTGG 2 cut(s) 283, 315
AluBI AGCT 1 cut(s) 422
AluI AGCT 1 cut(s) 422
AlwNI CAGNNNCTG 1 cut(s) 554
Aor51HI AGCGCT 1 cut(s) 496
AspLEI GCGC 1 cut(s) 497
AspS9I GGNCC 2 cut(s) 24, 202
AsuC2I CCSGG 1 cut(s) 130
AsuHPI GGTGA 2 cut(s) 118, 400
AvaII GGWCC 2 cut(s) 24, 202
BaeGI GKGCMC 1 cut(s) 362
BarI GAAGNNNNNNTAC 2 cut(s) 484, 516
BccI CCATC 2 cut(s) 281, 519
BceAI ACGGC 1 cut(s) 194
BcgI CGANNNNNNTGC 2 cut(s) 46, 80
BcnI CCSGG 1 cut(s) 130
BfoI RGCGCY 1 cut(s) 498
BisI GCNGC 1 cut(s) 31
BlsI GCNGC 1 cut(s) 32
Bme1390I CCNGG 1 cut(s) 130
Bme18I GGWCC 2 cut(s) 24, 202
BmgT120I GGNCC 2 cut(s) 24, 202
BmiI GGNNCC 2 cut(s) 26, 203
BmrFI CCNGG 1 cut(s) 130
BpuMI CCSGG 1 cut(s) 130
BsaBI GATNNNNATC 1 cut(s) 529
BsaJI CCNNGG 3 cut(s) 31, 98, 129
BsaWI WCCGGW 1 cut(s) 248
Bsc4I CCNNNNNNNGG 1 cut(s) 33
Bse3DI GCAATG 1 cut(s) 170
Bse8I GATNNNNATC 1 cut(s) 529
BseDI CCNNGG 3 cut(s) 31, 98, 129
BseGI GGATG 2 cut(s) 279, 530
BseJI GATNNNNATC 1 cut(s) 529
BseLI CCNNNNNNNGG 1 cut(s) 33
BseMI GCAATG 1 cut(s) 170
BseMII CTCAG 1 cut(s) 500
BseRI GAGGAG 1 cut(s) 432
BseSI GKGCMC 1 cut(s) 362
Bsh1236I CGCG 1 cut(s) 33
BsiSI CCGG 2 cut(s) 129, 249
BslFI GGGAC 2 cut(s) 10, 215
BslI CCNNNNNNNGG 1 cut(s) 33
BsmFI GGGAC 2 cut(s) 10, 215
Bsp1286I GDGCHC 1 cut(s) 362
Bsp1407I TGTACA 1 cut(s) 162
Bsp19I CCATGG 1 cut(s) 98
BspACI CCGC 5 cut(s) 28, 31, 33, 139, 513
BspCNI CTCAG 1 cut(s) 501
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 26, 203
BsrBI CCGCTC 1 cut(s) 513
BsrDI GCAATG 1 cut(s) 170
BsrGI TGTACA 1 cut(s) 162
BssECI CCNNGG 3 cut(s) 31, 98, 129
BssT1I CCWWGG 1 cut(s) 98
Bst6I CTCTTC 1 cut(s) 14
BstAUI TGTACA 1 cut(s) 162
BstDEI CTNAG 1 cut(s) 509
BstDSI CCRYGG 2 cut(s) 31, 98
BstF5I GGATG 2 cut(s) 279, 530
BstFNI CGCG 1 cut(s) 33
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 1 cut(s) 497
BstSCI CCNGG 1 cut(s) 128
BstSLI GKGCMC 1 cut(s) 362
BstUI CGCG 1 cut(s) 33
BtgI CCRYGG 2 cut(s) 31, 98
BtsCI GGATG 2 cut(s) 279, 530
CaiI CAGNNNCTG 1 cut(s) 554
CfoI GCGC 1 cut(s) 497
Cfr13I GGNCC 2 cut(s) 24, 202
Cfr42I CCGCGG 1 cut(s) 34
Csp6I GTAC 3 cut(s) 163, 295, 544
CviAII CATG 3 cut(s) 99, 346, 446
CviJI RGCY 3 cut(s) 394, 422, 434
CviKI_1 RGCY 3 cut(s) 394, 422, 434
CviQI GTAC 3 cut(s) 163, 295, 544
DdeI CTNAG 1 cut(s) 509
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
Eco130I CCWWGG 1 cut(s) 98
Eco32I GATATC 1 cut(s) 531
Eco47I GGWCC 2 cut(s) 24, 202
Eco47III AGCGCT 1 cut(s) 496
EcoO109I RGGNCCY 1 cut(s) 24
EcoRV GATATC 1 cut(s) 531
EcoT14I CCWWGG 1 cut(s) 98
ErhI CCWWGG 1 cut(s) 98
FaeI CATG 3 cut(s) 102, 349, 449
FaiI YATR 6 cut(s) 41, 100, 347, 384, 447, 459
FalI AAGNNNNNCTT 2 cut(s) 406, 438
FaqI GGGAC 2 cut(s) 10, 215
FatI CATG 3 cut(s) 98, 345, 445
FauI CCCGC 3 cut(s) 26, 35, 146
Fnu4HI GCNGC 1 cut(s) 31
FokI GGATG 2 cut(s) 266, 537
Fsp4HI GCNGC 1 cut(s) 31
GlaI GCGC 1 cut(s) 496
GluI GCNGC 1 cut(s) 31
HaeII RGCGCY 1 cut(s) 498
HapII CCGG 2 cut(s) 129, 249
HhaI GCGC 1 cut(s) 497
Hin1II CATG 3 cut(s) 102, 349, 449
Hin6I GCGC 1 cut(s) 495
HinP1I GCGC 1 cut(s) 495
HinfI GANTC 5 cut(s) 69, 186, 260, 409, 551
HpaII CCGG 2 cut(s) 129, 249
HphI GGTGA 2 cut(s) 118, 400
Hpy188I TCNGA 1 cut(s) 68
Hpy188III TCNNGA 1 cut(s) 439
HpyAV CCTTC 1 cut(s) 445
HpyCH4V TGCA 6 cut(s) 84, 175, 224, 345, 428, 481
HpyF3I CTNAG 1 cut(s) 509
Hsp92II CATG 3 cut(s) 102, 349, 449
HspAI GCGC 1 cut(s) 495
KspI CCGCGG 1 cut(s) 34
LmnI GCTCC 2 cut(s) 54, 419
LpnPI CCDG 4 cut(s) 142, 184, 262, 507
MbiI CCGCTC 1 cut(s) 513
MboII GAAGA 4 cut(s) 31, 225, 269, 294
MhlI GDGCHC 1 cut(s) 362
MluCI AATT 4 cut(s) 252, 333, 366, 454
MlyI GAGTC 2 cut(s) 180, 560
MnlI CCTC 5 cut(s) 15, 176, 212, 258, 410
MseI TTAA 1 cut(s) 462
MslI CAYNNNNRTG 1 cut(s) 97
MspA1I CMGCKG 1 cut(s) 33
MspI CCGG 2 cut(s) 129, 249
MspR9I CCNGG 1 cut(s) 130
MvnI CGCG 1 cut(s) 33
NciI CCSGG 1 cut(s) 130
NcoI CCATGG 1 cut(s) 98
NlaIII CATG 3 cut(s) 102, 349, 449
NlaIV GGNNCC 2 cut(s) 26, 203
PfeI GAWTC 3 cut(s) 69, 260, 409
PkrI GCNGC 1 cut(s) 32
PleI GAGTC 2 cut(s) 180, 559
PpsI GAGTC 2 cut(s) 180, 559
PpuMI RGGWCCY 1 cut(s) 24
PsiI TTATAA 1 cut(s) 384
Psp5II RGGWCCY 1 cut(s) 24
PspN4I GGNNCC 2 cut(s) 26, 203
PspPI GGNCC 2 cut(s) 24, 202
PspPPI RGGWCCY 1 cut(s) 24
PstNI CAGNNNCTG 1 cut(s) 554
RsaI GTAC 3 cut(s) 164, 296, 545
RsaNI GTAC 3 cut(s) 163, 295, 544
RseI CAYNNNNRTG 1 cut(s) 97
SacII CCGCGG 1 cut(s) 34
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 1 cut(s) 31
Sau96I GGNCC 2 cut(s) 24, 202
SchI GAGTC 2 cut(s) 180, 560
ScrFI CCNGG 1 cut(s) 130
SduI GDGCHC 1 cut(s) 362
SetI ASST 6 cut(s) 26, 223, 269, 390, 424, 488
Sfr303I CCGCGG 1 cut(s) 34
SgrBI CCGCGG 1 cut(s) 34
SinI GGWCC 2 cut(s) 24, 202
SmiMI CAYNNNNRTG 1 cut(s) 97
Sse9I AATT 4 cut(s) 252, 333, 366, 454
SsiI CCGC 5 cut(s) 28, 31, 33, 139, 513
SspI AATATT 1 cut(s) 378
StyD4I CCNGG 1 cut(s) 128
StyI CCWWGG 1 cut(s) 98
TaqI TCGA 1 cut(s) 263
TasI AATT 4 cut(s) 252, 333, 366, 454
TatI WGTACW 1 cut(s) 162
TauI GCSGC 1 cut(s) 33
TfiI GAWTC 3 cut(s) 69, 260, 409
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TspDTI ATGAA 1 cut(s) 17
VpaK11BI GGWCC 2 cut(s) 24, 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.