Rorug02G0176100

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0018781
Physical Location & Seq
Reverse (-)
16186440 .. 16190022
3583 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0176100.1

Sequence Viewer

Length: 1155 bp
ATGACCGCCGCTTCTACCACGAGAGAAGAGATACGAGACATGGGTTCGAGGTTTGGTCTGTTTCTCTTGGCAGTTTCCATTCTATGGGCACCCGTGCTCTCCGATCTAGTCATCTCCATAGTTGACCGACGCATTGATTTGACATCACAAATTGTGCACATCACTTCCACACTCAAGGTGGAGAATGAGGGAAGCGACTTGGTTACTGAGGTTTTGTTTTCTTTTCCCTCATGTTTTCGGGCAGAGCATTTGGCACATGTGGCGGCAACCTCCAGTGGAGGGAAAGGAGGGAAAGGAAAAGCCAAAGGTTCTAGTACTAGCTACGCTGTTCAACTTGTTAACCCTAGAGGCGTGCCTGCTTCCTTGACTTTTTACTCGGCTGACATTCAGCTCCTAGTGTTTCAGGATACTGCATTCTACCTCTCTCCTTATGCAGTCAAGGCACAAACACTCAGTGTTAAATTGCCTGATGCAGGGAACGAATCTTATATGAGAGTAGATAATACAAAGAATGTTGGTTCAGAGATCAAATATGGTCCATATGAGAATCTCCCTCCCTTTTCGTACTCACCTATTGTTGTTCATTGTGAAAGCAATCAACCCTTTGCTGTTGCTGAAGAGTTGGTGCGAGATATAGAGATTTCCCATTGGGGTAATGTTCAAGTGACAGAGCATTACAAATTTGTCCATGGTTGTGCCCAAAGCAAAGGAGAATTTTCAAGGCTTGATTATCAAGCCAGACCTCACGTGAGAGATCAGTATGCCATTGGCGTCTTGTTGCAAAGTTGCCACCAAGAACTCATTCTGTCTATTATAGAGACGAAATTGACAGAACTAGAAATTGAGCCTAGGTATCTTGTGACTGGTGGTTGGAAAACTGCATTTACCATTGGATATGGTTTGCTGCTTCATGACTTTTTATTTCAGTCTGATGGAAAATGCCTCATTGACATAACTTTTGGTTGCCCTATGAATGACGTGGTCATCAACACTCTCATTGTGAAGAGGACCACCCCGCCGATTCCTAAGAGCCATGACTACTCCTATGTCAATTCATACCGTACTCTCTACTCTGCTTTCCAGATAGCGTTGGAGGGCAGAACTTCAAGTAGCAATGAGTTAAAGGTTGAGAATACAGGAAAGCTAAGATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

384

Amino Acids

42.6

Weight (kDa)

6.14

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribophorin_I PF04597 38 - 253 7.5e-45 Ribophorin I
Ribophorin_I PF04597 275 - 339 3.8e-13 Ribophorin I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018781)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19921 FvH4_5g03280
rosa_laevigata RLG00000017921 RLG00000017922 RLG00000017924
rosa_multiflora Rmu_sc0000448.1_g000008
rosa_roxburghii Rroxscaffold_2G00132130
rosa_rugosa Rorug02G0176100
rosa_wichuraiana Rw2G017680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 88
AccB7I CCANNNNNTGG 1 cut(s) 84
AciI CCGC 4 cut(s) 6, 9, 263, 1016
AcsI RAATTY 2 cut(s) 680, 713
AcuI CTGAAG 1 cut(s) 636
AcvI CACGTG 1 cut(s) 748
AcyI GRCGYC 1 cut(s) 771
AdeI CACNNNGTG 1 cut(s) 455
AfaI GTAC 3 cut(s) 316, 566, 1063
AfiI CCNNNNNNNGG 3 cut(s) 84, 279, 473
AflIII ACRYGT 1 cut(s) 256
AgsI TTSAA 4 cut(s) 332, 662, 720, 1107
AjiI CACGTC 1 cut(s) 979
AjuI GAANNNNNNNTTGG 2 cut(s) 786, 818
AluBI AGCT 3 cut(s) 321, 391, 1144
AluI AGCT 3 cut(s) 321, 391, 1144
Alw21I GWGCWC 2 cut(s) 99, 159
Alw26I GTCTC 2 cut(s) 30, 812
Alw44I GTGCAC 1 cut(s) 155
ApaLI GTGCAC 1 cut(s) 155
ApeKI GCWGC 1 cut(s) 904
ApoI RAATTY 2 cut(s) 680, 713
ArsI GACNNNNNNTTYG 2 cut(s) 941, 973
Asp700I GAANNNNTTC 1 cut(s) 801
AspA2I CCTAGG 1 cut(s) 848
AspS9I GGNCC 2 cut(s) 536, 1008
AsuHPI GGTGA 1 cut(s) 561
AvaII GGWCC 2 cut(s) 536, 1008
AvrII CCTAGG 1 cut(s) 848
BaeGI GKGCMC 3 cut(s) 91, 159, 700
BanI GGYRCC 1 cut(s) 88
BauI CACGAG 1 cut(s) 19
BbrPI CACGTG 1 cut(s) 748
Bbv12I GWGCWC 2 cut(s) 99, 159
BbvI GCAGC 1 cut(s) 891
BccI CCATC 1 cut(s) 926
BciVI GTATCC 1 cut(s) 400
BcoDI GTCTC 2 cut(s) 30, 812
BfaI CTAG 7 cut(s) 107, 312, 318, 345, 395, 836, 849
BfuI GTATCC 1 cut(s) 400
BisI GCNGC 3 cut(s) 9, 264, 905
BlnI CCTAGG 1 cut(s) 848
BlsI GCNGC 3 cut(s) 10, 265, 906
BmcAI AGTACT 1 cut(s) 316
Bme18I GGWCC 2 cut(s) 536, 1008
BmgBI CACGTC 1 cut(s) 979
BmgT120I GGNCC 2 cut(s) 536, 1008
BmiI GGNNCC 1 cut(s) 90
BmsI GCATC 2 cut(s) 460, 1139
BpmI CTGGAG 1 cut(s) 256
BpuEI CTTGAG 1 cut(s) 158
BsaAI YACGTR 1 cut(s) 748
BsaHI GRCGYC 1 cut(s) 771
BsaJI CCNNGG 2 cut(s) 688, 848
Bsc4I CCNNNNNNNGG 3 cut(s) 84, 279, 473
Bse1I ACTGG 2 cut(s) 273, 868
Bse3DI GCAATG 1 cut(s) 1120
BseDI CCNNGG 2 cut(s) 688, 848
BseLI CCNNNNNNNGG 3 cut(s) 84, 279, 473
BseMI GCAATG 1 cut(s) 1120
BseMII CTCAG 2 cut(s) 198, 466
BseNI ACTGG 2 cut(s) 273, 868
BseSI GKGCMC 3 cut(s) 91, 159, 700
BseXI GCAGC 1 cut(s) 891
BshNI GGYRCC 1 cut(s) 88
BsiHKAI GWGCWC 2 cut(s) 99, 159
BslI CCNNNNNNNGG 3 cut(s) 84, 279, 473
BsmAI GTCTC 2 cut(s) 30, 812
BsmBI CGTCTC 1 cut(s) 812
BsmI GAATGC 1 cut(s) 413
Bsp1286I GDGCHC 4 cut(s) 91, 99, 159, 700
Bsp143I GATC 3 cut(s) 103, 525, 754
Bsp19I CCATGG 1 cut(s) 688
BspACI CCGC 4 cut(s) 6, 9, 263, 1016
BspCNI CTCAG 2 cut(s) 199, 465
BspHI TCATGA 1 cut(s) 910
BspLI GGNNCC 1 cut(s) 90
BspT107I GGYRCC 1 cut(s) 88
BsrDI GCAATG 1 cut(s) 1120
BsrI ACTGG 2 cut(s) 273, 868
BssECI CCNNGG 2 cut(s) 688, 848
BssMI GATC 3 cut(s) 103, 525, 754
BssNI GRCGYC 1 cut(s) 771
BssSI CACGAG 1 cut(s) 19
BssT1I CCWWGG 2 cut(s) 688, 848
Bst2BI CACGAG 1 cut(s) 19
Bst4CI ACNGT 1 cut(s) 1061
Bst6I CTCTTC 3 cut(s) 21, 612, 998
BstACI GRCGYC 1 cut(s) 771
BstBAI YACGTR 1 cut(s) 748
BstC8I GCNNGC 2 cut(s) 353, 357
BstDEI CTNAG 4 cut(s) 207, 452, 1026, 1145
BstDSI CCRYGG 1 cut(s) 688
BstENI CCTNNNNNAGG 1 cut(s) 471
BstKTI GATC 3 cut(s) 106, 528, 757
BstMAI GTCTC 2 cut(s) 30, 812
BstMBI GATC 3 cut(s) 103, 525, 754
BstMWI GCNNNNNNNGC 2 cut(s) 260, 440
BstNSI RCATGY 1 cut(s) 260
BstSLI GKGCMC 3 cut(s) 91, 159, 700
BstV1I GCAGC 1 cut(s) 891
BsuI GTATCC 1 cut(s) 400
BtgI CCRYGG 1 cut(s) 688
BtrI CACGTC 1 cut(s) 979
BtsIMutI CAGTG 2 cut(s) 280, 460
Cac8I GCNNGC 2 cut(s) 353, 357
CciI TCATGA 1 cut(s) 910
Cfr13I GGNCC 2 cut(s) 536, 1008
CseI GACGC 2 cut(s) 138, 760
Csp6I GTAC 3 cut(s) 315, 565, 1062
CviAII CATG 6 cut(s) 40, 231, 257, 689, 911, 1034
CviJI RGCY 9 cut(s) 302, 321, 380, 391, 724, 737, 847, 1032, 1144
CviKI_1 RGCY 9 cut(s) 302, 321, 380, 391, 724, 737, 847, 1032, 1144
CviQI GTAC 3 cut(s) 315, 565, 1062
DdeI CTNAG 4 cut(s) 207, 452, 1026, 1145
DpnI GATC 3 cut(s) 105, 527, 756
DpnII GATC 3 cut(s) 103, 525, 754
DraIII CACNNNGTG 1 cut(s) 455
Eam1104I CTCTTC 3 cut(s) 21, 612, 998
EarI CTCTTC 3 cut(s) 21, 612, 998
Eco130I CCWWGG 2 cut(s) 688, 848
Eco47I GGWCC 2 cut(s) 536, 1008
Eco57I CTGAAG 1 cut(s) 636
Eco72I CACGTG 1 cut(s) 748
EcoNI CCTNNNNNAGG 1 cut(s) 471
EcoT14I CCWWGG 2 cut(s) 688, 848
ErhI CCWWGG 2 cut(s) 688, 848
Esp3I CGTCTC 1 cut(s) 812
FaeI CATG 6 cut(s) 43, 234, 260, 692, 914, 1037
FatI CATG 6 cut(s) 39, 230, 256, 688, 910, 1033
FauI CCCGC 1 cut(s) 1023
FauNDI CATATG 1 cut(s) 541
Fnu4HI GCNGC 3 cut(s) 9, 264, 905
Fsp4HI GCNGC 3 cut(s) 9, 264, 905
FspBI CTAG 7 cut(s) 107, 312, 318, 345, 395, 836, 849
GluI GCNGC 3 cut(s) 9, 264, 905
GsuI CTGGAG 1 cut(s) 256
HgaI GACGC 2 cut(s) 138, 760
Hin1I GRCGYC 1 cut(s) 771
Hin1II CATG 6 cut(s) 43, 234, 260, 692, 914, 1037
HincII GTYRAC 2 cut(s) 124, 340
HindII GTYRAC 2 cut(s) 124, 340
HinfI GANTC 3 cut(s) 482, 547, 1021
HpaI GTTAAC 1 cut(s) 340
HphI GGTGA 1 cut(s) 561
Hpy166II GTNNAC 3 cut(s) 124, 157, 340
Hpy188I TCNGA 3 cut(s) 103, 523, 931
Hpy188III TCNNGA 3 cut(s) 404, 911, 1081
Hpy8I GTNNAC 3 cut(s) 124, 157, 340
Hpy99I CGWCG 1 cut(s) 132
HpyCH4III ACNGT 1 cut(s) 1061
HpyCH4IV ACGT 2 cut(s) 747, 978
HpyCH4V TGCA 6 cut(s) 157, 413, 434, 473, 781, 881
HpyF10VI GCNNNNNNNGC 2 cut(s) 260, 440
HpyF3I CTNAG 4 cut(s) 207, 452, 1026, 1145
HpySE526I ACGT 2 cut(s) 747, 978
Hsp92I GRCGYC 1 cut(s) 771
Hsp92II CATG 6 cut(s) 43, 234, 260, 692, 914, 1037
KspAI GTTAAC 1 cut(s) 340
Kzo9I GATC 3 cut(s) 103, 525, 754
LmnI GCTCC 1 cut(s) 396
LpnPI CCDG 9 cut(s) 286, 369, 389, 459, 480, 751, 849, 1094, 1122
Lsp1109I GCAGC 1 cut(s) 891
LweI GCATC 2 cut(s) 460, 1139
MaeI CTAG 7 cut(s) 107, 312, 318, 345, 395, 836, 849
MaeII ACGT 2 cut(s) 747, 978
MaeIII GTNAC 3 cut(s) 202, 664, 859
MalI GATC 3 cut(s) 105, 527, 756
MboI GATC 3 cut(s) 103, 525, 754
MboII GAAGA 3 cut(s) 38, 629, 1015
MhlI GDGCHC 4 cut(s) 91, 99, 159, 700
MluCI AATT 7 cut(s) 150, 461, 680, 713, 824, 840, 1051
MmeI TCCRAC 2 cut(s) 851, 1071
MroXI GAANNNNTTC 1 cut(s) 801
MseI TTAA 3 cut(s) 339, 459, 1121
MslI CAYNNNNRTG 1 cut(s) 693
Mva1269I GAATGC 1 cut(s) 413
MwoI GCNNNNNNNGC 2 cut(s) 260, 440
NcoI CCATGG 1 cut(s) 688
NdeI CATATG 1 cut(s) 541
NdeII GATC 3 cut(s) 103, 525, 754
NlaIII CATG 6 cut(s) 43, 234, 260, 692, 914, 1037
NlaIV GGNNCC 1 cut(s) 90
NmeAIII GCCGAG 1 cut(s) 356
NmuCI GTSAC 2 cut(s) 664, 859
NspI RCATGY 1 cut(s) 260
PagI TCATGA 1 cut(s) 910
PciI ACATGT 1 cut(s) 256
PctI GAATGC 1 cut(s) 413
PdmI GAANNNNTTC 1 cut(s) 801
PfeI GAWTC 3 cut(s) 482, 547, 1021
PflFI GACNNNGTC 1 cut(s) 980
PflMI CCANNNNNTGG 1 cut(s) 84
PkrI GCNGC 3 cut(s) 10, 265, 906
PmaCI CACGTG 1 cut(s) 748
PmlI CACGTG 1 cut(s) 748
Ppu21I YACGTR 1 cut(s) 748
PscI ACATGT 1 cut(s) 256
PspCI CACGTG 1 cut(s) 748
PspN4I GGNNCC 1 cut(s) 90
PspPI GGNCC 2 cut(s) 536, 1008
PsyI GACNNNGTC 1 cut(s) 980
RsaI GTAC 3 cut(s) 316, 566, 1063
RsaNI GTAC 3 cut(s) 315, 565, 1062
RseI CAYNNNNRTG 1 cut(s) 693
SaqAI TTAA 3 cut(s) 339, 459, 1121
SatI GCNGC 3 cut(s) 9, 264, 905
Sau3AI GATC 3 cut(s) 103, 525, 754
Sau96I GGNCC 2 cut(s) 536, 1008
ScaI AGTACT 1 cut(s) 316
SduI GDGCHC 4 cut(s) 91, 99, 159, 700
SfaNI GCATC 2 cut(s) 460, 1139
SinI GGWCC 2 cut(s) 536, 1008
SmiMI CAYNNNNRTG 1 cut(s) 693
SmlI CTYRAG 1 cut(s) 173
SmoI CTYRAG 1 cut(s) 173
Sse9I AATT 7 cut(s) 150, 461, 680, 713, 824, 840, 1051
SsiI CCGC 4 cut(s) 6, 9, 263, 1016
SspMI CTAG 7 cut(s) 107, 312, 318, 345, 395, 836, 849
StyI CCWWGG 2 cut(s) 688, 848
TaaI ACNGT 1 cut(s) 1061
TaiI ACGT 2 cut(s) 750, 981
TaqI TCGA 1 cut(s) 47
TaqII GACCGA 1 cut(s) 141
TasI AATT 7 cut(s) 150, 461, 680, 713, 824, 840, 1051
TatI WGTACW 1 cut(s) 314
TauI GCSGC 2 cut(s) 11, 266
TfiI GAWTC 3 cut(s) 482, 547, 1021
Tru1I TTAA 3 cut(s) 339, 459, 1121
Tru9I TTAA 3 cut(s) 339, 459, 1121
TscAI CASTG 2 cut(s) 280, 460
TseFI GTSAC 2 cut(s) 664, 859
TseI GCWGC 1 cut(s) 904
Tsp45I GTSAC 2 cut(s) 664, 859
TspDTI ATGAA 4 cut(s) 572, 899, 986, 1044
TspRI CASTG 2 cut(s) 280, 460
Tth111I GACNNNGTC 1 cut(s) 980
Van91I CCANNNNNTGG 1 cut(s) 84
VneI GTGCAC 1 cut(s) 155
VpaK11BI GGWCC 2 cut(s) 536, 1008
XagI CCTNNNNNAGG 1 cut(s) 471
XapI RAATTY 2 cut(s) 680, 713
XceI RCATGY 1 cut(s) 260
XcmI CCANNNNNNNNNTGG 1 cut(s) 175
XmaJI CCTAGG 1 cut(s) 848
XmnI GAANNNNTTC 1 cut(s) 801
XspI CTAG 7 cut(s) 107, 312, 318, 345, 395, 836, 849
ZrmI AGTACT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.