Rw2G017680

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0018781
Physical Location & Seq
Forward (+)
21893322 .. 21894430
1109 bp
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UTR
Exon/CDS
Intron
Rw2G017680.1

Sequence Viewer

Length: 603 bp
ATGTTGGCAAAATTGCTCGTGGTAATTTATTTTGTGTACGTAGGGAGCAGATTGTTCGGAATCACTTTCAAATGCACCAAAACACAACCATGGAAATACGATGTGTTTTTGAGTTTCACCGGGGAAACCCGCAAGGGTTTCACTCACTTCTTGTACAAAGCATTGCAACGGCGAGGGCTCAAAGTTTTCAGGGACCAAGAAATCCAAAGAGGTGCAAACATTTCTTCAATGCTCTCAACTGGAATTGAAGAATCGAGGTTTGCCATCATCATCCTTTCGCCAAAGTACGCTTCTTCTACTTGGTGCTTGGACGAACTTACTAAAATTGTGGAATGCATGGAAGTCAAGGGCACAAGAATTCTCCCAATATTTTATAAGGTGAAGCCAGCGAGTGTACGGAATCAAAGAGGAGCTTTTGCAAAAGCCTTCAAGAAGCATGATAATAATTATGTTAAAGACAAAGCAAAAGTGCAAAGGTGGAGAAGCGCTTTTACAAAACTGAGCGGTATCGCTGGATGGATATCAACAGAATGGTACTACGAGCTTATTGAAGAAGTCGTGGAATTAGTGTGTAGCACATGCATCTTAGCTCGATCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

200

Amino Acids

23.13

Weight (kDa)

9.76

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 32 - 188 6.2e-46 TIR domain
TIR_2 PF13676 35 - 130 1.3e-18 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018781)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19921 FvH4_5g03280
rosa_laevigata RLG00000017921 RLG00000017922 RLG00000017924
rosa_multiflora Rmu_sc0000448.1_g000008
rosa_roxburghii Rroxscaffold_2G00132130
rosa_rugosa Rorug02G0176100
rosa_wichuraiana Rw2G017680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
AccBSI CCGCTC 1 cut(s) 504
AciI CCGC 2 cut(s) 130, 504
AclWI GGATC 1 cut(s) 588
AcsI RAATTY 1 cut(s) 357
AfaI GTAC 5 cut(s) 38, 155, 287, 396, 536
AfeI AGCGCT 1 cut(s) 487
AgsI TTSAA 5 cut(s) 70, 228, 248, 430, 551
AjuI GAANNNNNNNTTGG 2 cut(s) 274, 306
AluBI AGCT 3 cut(s) 413, 544, 590
AluI AGCT 3 cut(s) 413, 544, 590
AlwI GGATC 1 cut(s) 588
Aor51HI AGCGCT 1 cut(s) 487
ApoI RAATTY 1 cut(s) 357
AspLEI GCGC 1 cut(s) 488
AspS9I GGNCC 1 cut(s) 193
AsuC2I CCSGG 1 cut(s) 121
AsuHPI GGTGA 2 cut(s) 109, 391
AvaII GGWCC 1 cut(s) 193
BaeGI GKGCMC 1 cut(s) 353
BanII GRGCYC 1 cut(s) 180
BarI GAAGNNNNNNTAC 2 cut(s) 475, 507
BauI CACGAG 1 cut(s) 17
BccI CCATC 2 cut(s) 272, 510
BceAI ACGGC 1 cut(s) 185
BcgI CGANNNNNNTGC 2 cut(s) 37, 71
BcnI CCSGG 1 cut(s) 121
BfoI RGCGCY 1 cut(s) 489
Bme1390I CCNGG 1 cut(s) 121
Bme18I GGWCC 1 cut(s) 193
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 1 cut(s) 194
BmrFI CCNGG 1 cut(s) 121
BmsI GCATC 1 cut(s) 591
BpuMI CCSGG 1 cut(s) 121
BsaAI YACGTR 1 cut(s) 40
BsaBI GATNNNNATC 1 cut(s) 520
BsaJI CCNNGG 2 cut(s) 89, 120
Bse1I ACTGG 1 cut(s) 244
Bse3DI GCAATG 1 cut(s) 161
Bse8I GATNNNNATC 1 cut(s) 520
BseDI CCNNGG 2 cut(s) 89, 120
BseGI GGATG 2 cut(s) 270, 521
BseJI GATNNNNATC 1 cut(s) 520
BseMI GCAATG 1 cut(s) 161
BseMII CTCAG 1 cut(s) 491
BseNI ACTGG 1 cut(s) 244
BseRI GAGGAG 1 cut(s) 423
BseSI GKGCMC 1 cut(s) 353
BsiSI CCGG 1 cut(s) 120
BslFI GGGAC 1 cut(s) 206
BsmFI GGGAC 1 cut(s) 206
BsmI GAATGC 1 cut(s) 338
Bsp1286I GDGCHC 2 cut(s) 180, 353
Bsp1407I TGTACA 1 cut(s) 153
Bsp143I GATC 1 cut(s) 593
Bsp19I CCATGG 1 cut(s) 89
BspACI CCGC 2 cut(s) 130, 504
BspCNI CTCAG 1 cut(s) 492
BspLI GGNNCC 1 cut(s) 194
BspPI GGATC 1 cut(s) 588
BsrBI CCGCTC 1 cut(s) 504
BsrDI GCAATG 1 cut(s) 161
BsrGI TGTACA 1 cut(s) 153
BsrI ACTGG 1 cut(s) 244
BssECI CCNNGG 2 cut(s) 89, 120
BssMI GATC 1 cut(s) 593
BssSI CACGAG 1 cut(s) 17
BssT1I CCWWGG 1 cut(s) 89
Bst2BI CACGAG 1 cut(s) 17
BstAUI TGTACA 1 cut(s) 153
BstBAI YACGTR 1 cut(s) 40
BstC8I GCNNGC 1 cut(s) 387
BstDEI CTNAG 2 cut(s) 500, 586
BstDSI CCRYGG 1 cut(s) 89
BstF5I GGATG 2 cut(s) 270, 521
BstH2I RGCGCY 1 cut(s) 489
BstHHI GCGC 1 cut(s) 488
BstKTI GATC 1 cut(s) 596
BstMBI GATC 1 cut(s) 593
BstNSI RCATGY 1 cut(s) 582
BstSCI CCNGG 1 cut(s) 119
BstSLI GKGCMC 1 cut(s) 353
BstSNI TACGTA 1 cut(s) 40
BtgI CCRYGG 1 cut(s) 89
BtsCI GGATG 2 cut(s) 270, 521
Cac8I GCNNGC 1 cut(s) 387
CfoI GCGC 1 cut(s) 488
Cfr13I GGNCC 1 cut(s) 193
Csp6I GTAC 5 cut(s) 37, 154, 286, 395, 535
CviAII CATG 4 cut(s) 90, 337, 437, 579
CviJI RGCY 6 cut(s) 178, 385, 413, 425, 544, 590
CviKI_1 RGCY 6 cut(s) 178, 385, 413, 425, 544, 590
CviQI GTAC 5 cut(s) 37, 154, 286, 395, 535
DdeI CTNAG 2 cut(s) 500, 586
DpnI GATC 1 cut(s) 595
DpnII GATC 1 cut(s) 593
Eco105I TACGTA 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 89
Eco24I GRGCYC 1 cut(s) 180
Eco32I GATATC 1 cut(s) 522
Eco47I GGWCC 1 cut(s) 193
Eco47III AGCGCT 1 cut(s) 487
EcoRI GAATTC 1 cut(s) 357
EcoRV GATATC 1 cut(s) 522
EcoT14I CCWWGG 1 cut(s) 89
EcoT22I ATGCAT 2 cut(s) 338, 584
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 89
FaeI CATG 4 cut(s) 93, 340, 440, 582
FaiI YATR 6 cut(s) 91, 338, 375, 438, 450, 580
FalI AAGNNNNNCTT 2 cut(s) 397, 429
FaqI GGGAC 1 cut(s) 206
FatI CATG 4 cut(s) 89, 336, 436, 578
FauI CCCGC 1 cut(s) 137
FokI GGATG 2 cut(s) 257, 528
FriOI GRGCYC 1 cut(s) 180
GlaI GCGC 1 cut(s) 487
HaeII RGCGCY 1 cut(s) 489
HapII CCGG 1 cut(s) 120
HhaI GCGC 1 cut(s) 488
Hin1II CATG 4 cut(s) 93, 340, 440, 582
Hin6I GCGC 1 cut(s) 486
HinP1I GCGC 1 cut(s) 486
HinfI GANTC 3 cut(s) 60, 251, 400
HpaII CCGG 1 cut(s) 120
HphI GGTGA 2 cut(s) 109, 391
Hpy166II GTNNAC 2 cut(s) 37, 395
Hpy188I TCNGA 1 cut(s) 59
Hpy188III TCNNGA 1 cut(s) 430
Hpy8I GTNNAC 2 cut(s) 37, 395
HpyAV CCTTC 1 cut(s) 436
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 7 cut(s) 75, 166, 215, 336, 419, 472, 582
HpyF3I CTNAG 2 cut(s) 500, 586
HpySE526I ACGT 1 cut(s) 39
Hsp92II CATG 4 cut(s) 93, 340, 440, 582
HspAI GCGC 1 cut(s) 486
Kzo9I GATC 1 cut(s) 593
LmnI GCTCC 2 cut(s) 45, 410
LpnPI CCDG 5 cut(s) 133, 175, 225, 399, 498
LweI GCATC 1 cut(s) 591
MaeII ACGT 1 cut(s) 39
MalI GATC 1 cut(s) 595
MbiI CCGCTC 1 cut(s) 504
MboI GATC 1 cut(s) 593
MboII GAAGA 4 cut(s) 216, 260, 285, 563
MhlI GDGCHC 2 cut(s) 180, 353
MluCI AATT 7 cut(s) 11, 24, 243, 324, 357, 445, 563
MnlI CCTC 4 cut(s) 167, 203, 249, 401
Mph1103I ATGCAT 2 cut(s) 338, 584
MseI TTAA 1 cut(s) 453
MslI CAYNNNNRTG 1 cut(s) 88
MspI CCGG 1 cut(s) 120
MspR9I CCNGG 1 cut(s) 121
Mva1269I GAATGC 1 cut(s) 338
NciI CCSGG 1 cut(s) 121
NcoI CCATGG 1 cut(s) 89
NdeII GATC 1 cut(s) 593
NlaIII CATG 4 cut(s) 93, 340, 440, 582
NlaIV GGNNCC 1 cut(s) 194
NsiI ATGCAT 2 cut(s) 338, 584
NspI RCATGY 1 cut(s) 582
PctI GAATGC 1 cut(s) 338
PfeI GAWTC 3 cut(s) 60, 251, 400
Ppu21I YACGTR 1 cut(s) 40
PsiI TTATAA 1 cut(s) 375
PspN4I GGNNCC 1 cut(s) 194
PspPI GGNCC 1 cut(s) 193
RsaI GTAC 5 cut(s) 38, 155, 287, 396, 536
RsaNI GTAC 5 cut(s) 37, 154, 286, 395, 535
RseI CAYNNNNRTG 1 cut(s) 88
SaqAI TTAA 1 cut(s) 453
Sau3AI GATC 1 cut(s) 593
Sau96I GGNCC 1 cut(s) 193
ScrFI CCNGG 1 cut(s) 121
SduI GDGCHC 2 cut(s) 180, 353
SetI ASST 8 cut(s) 42, 214, 260, 381, 415, 479, 546, 592
SfaNI GCATC 1 cut(s) 591
SinI GGWCC 1 cut(s) 193
SmiMI CAYNNNNRTG 1 cut(s) 88
SnaBI TACGTA 1 cut(s) 40
Sse9I AATT 7 cut(s) 11, 24, 243, 324, 357, 445, 563
SsiI CCGC 2 cut(s) 130, 504
SspI AATATT 1 cut(s) 369
StyD4I CCNGG 1 cut(s) 119
StyI CCWWGG 1 cut(s) 89
TaiI ACGT 1 cut(s) 42
TaqI TCGA 2 cut(s) 254, 592
TasI AATT 7 cut(s) 11, 24, 243, 324, 357, 445, 563
TatI WGTACW 1 cut(s) 153
TfiI GAWTC 3 cut(s) 60, 251, 400
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TspGWI ACGGA 1 cut(s) 412
VpaK11BI GGWCC 1 cut(s) 193
XapI RAATTY 1 cut(s) 357
XceI RCATGY 1 cut(s) 582
Zsp2I ATGCAT 2 cut(s) 338, 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.