WEB family
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT4G40020 |
| fragaria_vesca | FvH4_1g20290 |
| malus_domestica | MD03G1184100.v1.1 MD11G1200900.v1.1 |
| prunus_persica | Prupe.6G192500_v2.0.a1 |
| pyrus_communis | pycom03g13740 |
| rosa_chinensis | RchiOBHm_Chr2g0112431 |
| rosa_laevigata | RLG00000017980 |
| rosa_multiflora | Rmu_co8429047.1_g000001 Rmu_sc0004467.1_g000073 Rmu_sc0004467.1_g000074 |
| rosa_roxburghii | Rroxscaffold_2G00131450 |
| rosa_rugosa | Rorug02G0180500 |
| rosa_samantha | Rh2AG233900 Rh2BG247200 Rh2CG238400 Rh2DG241500 |
| rosa_wichuraiana | Rw2G018110 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2126 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 451 |
| AccII | CGCG | 1 cut(s) | 1452 |
| AciI | CCGC | 4 cut(s) | 136, 1500, 1642, 1697 |
| AclWI | GGATC | 3 cut(s) | 2168, 2181, 2229 |
| AcoI | YGGCCR | 1 cut(s) | 204 |
| AcsI | RAATTY | 7 cut(s) | 291, 378, 471, 645, 763, 1522, 1567 |
| AcuI | CTGAAG | 5 cut(s) | 45, 896, 1037, 1056, 1825 |
| AfaI | GTAC | 3 cut(s) | 1247, 1956, 2137 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 2032 |
| AjnI | CCWGG | 2 cut(s) | 1262, 1542 |
| Alw21I | GWGCWC | 4 cut(s) | 222, 396, 1003, 1149 |
| Alw26I | GTCTC | 5 cut(s) | 345, 902, 1397, 1850, 1965 |
| Alw44I | GTGCAC | 1 cut(s) | 999 |
| AlwI | GGATC | 3 cut(s) | 2168, 2181, 2229 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 313 |
| Ama87I | CYCGRG | 5 cut(s) | 656, 1400, 1512, 1578, 2083 |
| AoxI | GGCC | 5 cut(s) | 204, 1416, 1467, 1560, 1761 |
| ApaLI | GTGCAC | 1 cut(s) | 999 |
| ApeKI | GCWGC | 5 cut(s) | 4, 304, 1259, 1361, 1613 |
| ApoI | RAATTY | 7 cut(s) | 291, 378, 471, 645, 763, 1522, 1567 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 291, 378, 471, 645 |
| AspLEI | GCGC | 1 cut(s) | 1452 |
| AspS9I | GGNCC | 1 cut(s) | 903 |
| AsuC2I | CCSGG | 1 cut(s) | 1965 |
| AsuHPI | GGTGA | 5 cut(s) | 1567, 1612, 1919, 1973, 2160 |
| AvaI | CYCGRG | 5 cut(s) | 656, 1400, 1512, 1578, 2083 |
| AvaII | GGWCC | 1 cut(s) | 903 |
| BaeGI | GKGCMC | 1 cut(s) | 1003 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 855, 888 |
| BalI | TGGCCA | 1 cut(s) | 206 |
| BamHI | GGATCC | 1 cut(s) | 2173 |
| BanII | GRGCYC | 3 cut(s) | 88, 222, 396 |
| BauI | CACGAG | 1 cut(s) | 1010 |
| BbsI | GAAGAC | 1 cut(s) | 1353 |
| Bbv12I | GWGCWC | 4 cut(s) | 222, 396, 1003, 1149 |
| BbvCI | CCTCAGC | 1 cut(s) | 249 |
| BbvI | GCAGC | 5 cut(s) | 16, 316, 1246, 1348, 1600 |
| BccI | CCATC | 6 cut(s) | 655, 1898, 2034, 2057, 2061, 2173 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1340, 1374 |
| BciT130I | CCWGG | 2 cut(s) | 1264, 1544 |
| BclI | TGATCA | 2 cut(s) | 711, 2041 |
| BcnI | CCSGG | 1 cut(s) | 1965 |
| BcoDI | GTCTC | 5 cut(s) | 345, 902, 1397, 1850, 1965 |
| BfaI | CTAG | 3 cut(s) | 494, 2048, 2060 |
| BfmI | CTRYAG | 2 cut(s) | 1121, 1378 |
| BisI | GCNGC | 7 cut(s) | 5, 137, 305, 1260, 1362, 1500, 1614 |
| BlpI | GCTNAGC | 2 cut(s) | 833, 1969 |
| BlsI | GCNGC | 7 cut(s) | 6, 138, 306, 1261, 1363, 1501, 1615 |
| BmcAI | AGTACT | 1 cut(s) | 2137 |
| Bme1390I | CCNGG | 3 cut(s) | 1264, 1544, 1965 |
| Bme18I | GGWCC | 1 cut(s) | 903 |
| BmeT110I | CYCGRG | 5 cut(s) | 656, 1400, 1512, 1578, 2083 |
| BmgT120I | GGNCC | 1 cut(s) | 903 |
| BmiI | GGNNCC | 5 cut(s) | 2163, 2175, 2211, 2235, 2259 |
| BmrFI | CCNGG | 3 cut(s) | 1264, 1544, 1965 |
| BmsI | GCATC | 1 cut(s) | 415 |
| BpiI | GAAGAC | 1 cut(s) | 1353 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2007, 2039 |
| BpmI | CTGGAG | 1 cut(s) | 752 |
| Bpu10I | CCTNAGC | 2 cut(s) | 249, 429 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 833, 1969 |
| BpuEI | CTTGAG | 3 cut(s) | 1007, 1135, 1832 |
| BpuMI | CCSGG | 1 cut(s) | 1965 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2040 |
| BsaJI | CCNNGG | 4 cut(s) | 741, 1092, 1725, 1764 |
| BsaWI | WCCGGW | 2 cut(s) | 1983, 2170 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 797, 827 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 2032 |
| Bse1I | ACTGG | 4 cut(s) | 231, 735, 1321, 2247 |
| Bse3DI | GCAATG | 2 cut(s) | 1062, 1506 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2040 |
| BseBI | CCWGG | 2 cut(s) | 1264, 1544 |
| BseDI | CCNNGG | 4 cut(s) | 741, 1092, 1725, 1764 |
| BseGI | GGATG | 5 cut(s) | 889, 1066, 1225, 1714, 2026 |
| BseJI | GATNNNNATC | 1 cut(s) | 2040 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 2032 |
| BseMI | GCAATG | 2 cut(s) | 1062, 1506 |
| BseNI | ACTGG | 4 cut(s) | 231, 735, 1321, 2247 |
| BseRI | GAGGAG | 4 cut(s) | 821, 824, 1004, 1571 |
| BseSI | GKGCMC | 1 cut(s) | 1003 |
| BseXI | GCAGC | 5 cut(s) | 16, 316, 1246, 1348, 1600 |
| BsgI | GTGCAG | 3 cut(s) | 570, 839, 1347 |
| Bsh1236I | CGCG | 1 cut(s) | 1452 |
| BshFI | GGCC | 5 cut(s) | 206, 1418, 1469, 1562, 1763 |
| BsiHKAI | GWGCWC | 4 cut(s) | 222, 396, 1003, 1149 |
| BsiHKCI | CYCGRG | 5 cut(s) | 656, 1400, 1512, 1578, 2083 |
| BsiSI | CCGG | 3 cut(s) | 1965, 1984, 2171 |
| BslFI | GGGAC | 1 cut(s) | 2080 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 2032 |
| BsmAI | GTCTC | 5 cut(s) | 345, 902, 1397, 1850, 1965 |
| BsmBI | CGTCTC | 1 cut(s) | 1397 |
| BsmFI | GGGAC | 1 cut(s) | 2080 |
| BsmI | GAATGC | 2 cut(s) | 328, 2000 |
| BsnI | GGCC | 5 cut(s) | 206, 1418, 1469, 1562, 1763 |
| BsoBI | CYCGRG | 5 cut(s) | 656, 1400, 1512, 1578, 2083 |
| Bsp1286I | GDGCHC | 5 cut(s) | 88, 222, 396, 1003, 1149 |
| Bsp143I | GATC | 9 cut(s) | 34, 79, 573, 711, 1944, 2041, 2119, 2173, 2221 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 833, 1969 |
| BspACI | CCGC | 4 cut(s) | 136, 1500, 1642, 1697 |
| BspANI | GGCC | 5 cut(s) | 206, 1418, 1469, 1562, 1763 |
| BspFNI | CGCG | 1 cut(s) | 1452 |
| BspHI | TCATGA | 1 cut(s) | 1888 |
| BspLI | GGNNCC | 5 cut(s) | 2163, 2175, 2211, 2235, 2259 |
| BspMAI | CTGCAG | 1 cut(s) | 1125 |
| BspPI | GGATC | 3 cut(s) | 2168, 2181, 2229 |
| BspQI | GCTCTTC | 1 cut(s) | 1333 |
| BsrDI | GCAATG | 2 cut(s) | 1062, 1506 |
| BsrI | ACTGG | 4 cut(s) | 231, 735, 1321, 2247 |
| BssECI | CCNNGG | 4 cut(s) | 741, 1092, 1725, 1764 |
| BssMI | GATC | 9 cut(s) | 34, 79, 573, 711, 1944, 2041, 2119, 2173, 2221 |
| BssSI | CACGAG | 1 cut(s) | 1010 |
| BssT1I | CCWWGG | 3 cut(s) | 741, 1725, 1764 |
| Bst2BI | CACGAG | 1 cut(s) | 1010 |
| Bst2UI | CCWGG | 2 cut(s) | 1264, 1544 |
| Bst4CI | ACNGT | 2 cut(s) | 402, 1959 |
| Bst6I | CTCTTC | 4 cut(s) | 21, 786, 1278, 1333 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 1393, 1487, 1508 |
| BstDSI | CCRYGG | 1 cut(s) | 1092 |
| BstF5I | GGATG | 5 cut(s) | 889, 1066, 1225, 1714, 2026 |
| BstFNI | CGCG | 1 cut(s) | 1452 |
| BstHHI | GCGC | 1 cut(s) | 1452 |
| BstKTI | GATC | 9 cut(s) | 37, 82, 576, 714, 1947, 2044, 2122, 2176, 2224 |
| BstMAI | GTCTC | 5 cut(s) | 345, 902, 1397, 1850, 1965 |
| BstMBI | GATC | 9 cut(s) | 34, 79, 573, 711, 1944, 2041, 2119, 2173, 2221 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 125, 1097, 1393, 1487, 1496, 1508 |
| BstNI | CCWGG | 2 cut(s) | 1264, 1544 |
| BstNSI | RCATGY | 3 cut(s) | 62, 136, 1361 |
| BstSCI | CCNGG | 3 cut(s) | 1262, 1542, 1963 |
| BstSFI | CTRYAG | 2 cut(s) | 1121, 1378 |
| BstSLI | GKGCMC | 1 cut(s) | 1003 |
| BstUI | CGCG | 1 cut(s) | 1452 |
| BstV1I | GCAGC | 5 cut(s) | 16, 316, 1246, 1348, 1600 |
| BstV2I | GAAGAC | 1 cut(s) | 1353 |
| BstX2I | RGATCY | 2 cut(s) | 2173, 2221 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 214 |
| BstYI | RGATCY | 2 cut(s) | 2173, 2221 |
| BsuRI | GGCC | 5 cut(s) | 206, 1418, 1469, 1562, 1763 |
| BtgI | CCRYGG | 1 cut(s) | 1092 |
| BtsCI | GGATG | 5 cut(s) | 889, 1066, 1225, 1714, 2026 |
| BtsI | GCAGTG | 3 cut(s) | 832, 1118, 1254 |
| BtsIMutI | CAGTG | 4 cut(s) | 832, 1118, 1254, 2240 |
| CaiI | CAGNNNCTG | 1 cut(s) | 313 |
| CciI | TCATGA | 1 cut(s) | 1888 |
| CfoI | GCGC | 1 cut(s) | 1452 |
| Cfr13I | GGNCC | 1 cut(s) | 903 |
| CseI | GACGC | 2 cut(s) | 951, 2207 |
| Csp6I | GTAC | 3 cut(s) | 1246, 1955, 2136 |
| CviAII | CATG | 9 cut(s) | 59, 133, 860, 877, 1299, 1358, 1388, 1420, 1889 |
| CviQI | GTAC | 3 cut(s) | 1246, 1955, 2136 |
| DpnI | GATC | 9 cut(s) | 36, 81, 575, 713, 1946, 2043, 2121, 2175, 2223 |
| DpnII | GATC | 9 cut(s) | 34, 79, 573, 711, 1944, 2041, 2119, 2173, 2221 |
| DraI | TTTAAA | 1 cut(s) | 1207 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 451 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 451 |
| EaeI | YGGCCR | 1 cut(s) | 204 |
| Eam1104I | CTCTTC | 4 cut(s) | 21, 786, 1278, 1333 |
| EarI | CTCTTC | 4 cut(s) | 21, 786, 1278, 1333 |
| Ecl136II | GAGCTC | 2 cut(s) | 220, 394 |
| Eco130I | CCWWGG | 3 cut(s) | 741, 1725, 1764 |
| Eco147I | AGGCCT | 2 cut(s) | 1469, 1562 |
| Eco24I | GRGCYC | 3 cut(s) | 88, 222, 396 |
| Eco47I | GGWCC | 1 cut(s) | 903 |
| Eco53kI | GAGCTC | 2 cut(s) | 220, 394 |
| Eco57I | CTGAAG | 5 cut(s) | 45, 896, 1037, 1056, 1825 |
| Eco88I | CYCGRG | 5 cut(s) | 656, 1400, 1512, 1578, 2083 |
| EcoICRI | GAGCTC | 2 cut(s) | 220, 394 |
| EcoRI | GAATTC | 4 cut(s) | 291, 378, 471, 645 |
| EcoRII | CCWGG | 2 cut(s) | 1262, 1542 |
| EcoT14I | CCWWGG | 3 cut(s) | 741, 1725, 1764 |
| EcoT38I | GRGCYC | 3 cut(s) | 88, 222, 396 |
| ErhI | CCWWGG | 3 cut(s) | 741, 1725, 1764 |
| Esp3I | CGTCTC | 1 cut(s) | 1397 |
| FaeI | CATG | 9 cut(s) | 62, 136, 863, 880, 1302, 1361, 1391, 1423, 1892 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 180, 212, 517, 549, 2084, 2116, 2125, 2157 |
| FaqI | GGGAC | 1 cut(s) | 2080 |
| FatI | CATG | 9 cut(s) | 58, 132, 859, 876, 1298, 1357, 1387, 1419, 1888 |
| FauNDI | CATATG | 1 cut(s) | 12 |
| FbaI | TGATCA | 2 cut(s) | 711, 2041 |
| Fnu4HI | GCNGC | 7 cut(s) | 5, 137, 305, 1260, 1362, 1500, 1614 |
| FokI | GGATG | 5 cut(s) | 896, 1073, 1232, 1721, 2013 |
| FriOI | GRGCYC | 3 cut(s) | 88, 222, 396 |
| Fsp4HI | GCNGC | 7 cut(s) | 5, 137, 305, 1260, 1362, 1500, 1614 |
| FspBI | CTAG | 3 cut(s) | 494, 2048, 2060 |
| GlaI | GCGC | 1 cut(s) | 1451 |
| GluI | GCNGC | 7 cut(s) | 5, 137, 305, 1260, 1362, 1500, 1614 |
| GsuI | CTGGAG | 1 cut(s) | 752 |
| HaeIII | GGCC | 5 cut(s) | 206, 1418, 1469, 1562, 1763 |
| HapII | CCGG | 3 cut(s) | 1965, 1984, 2171 |
| HgaI | GACGC | 2 cut(s) | 951, 2207 |
| HhaI | GCGC | 1 cut(s) | 1452 |
| Hin1II | CATG | 9 cut(s) | 62, 136, 863, 880, 1302, 1361, 1391, 1423, 1892 |
| Hin6I | GCGC | 1 cut(s) | 1450 |
| HinP1I | GCGC | 1 cut(s) | 1450 |
| HincII | GTYRAC | 3 cut(s) | 156, 918, 1023 |
| HindII | GTYRAC | 3 cut(s) | 156, 918, 1023 |
| HpaII | CCGG | 3 cut(s) | 1965, 1984, 2171 |
| HphI | GGTGA | 5 cut(s) | 1567, 1612, 1919, 1973, 2160 |
| Hpy166II | GTNNAC | 4 cut(s) | 156, 918, 1001, 1023 |
| Hpy8I | GTNNAC | 4 cut(s) | 156, 918, 1001, 1023 |
| HpyCH4III | ACNGT | 2 cut(s) | 402, 1959 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 125, 1097, 1393, 1487, 1496, 1508 |
| Hsp92II | CATG | 9 cut(s) | 62, 136, 863, 880, 1302, 1361, 1391, 1423, 1892 |
| HspAI | GCGC | 1 cut(s) | 1450 |
| Ksp22I | TGATCA | 2 cut(s) | 711, 2041 |
| Kzo9I | GATC | 9 cut(s) | 34, 79, 573, 711, 1944, 2041, 2119, 2173, 2221 |
| LguI | GCTCTTC | 1 cut(s) | 1333 |
| LmnI | GCTCC | 8 cut(s) | 16, 217, 391, 733, 952, 1852, 2161, 2209 |
| Lsp1109I | GCAGC | 5 cut(s) | 16, 316, 1246, 1348, 1600 |
| LweI | GCATC | 1 cut(s) | 415 |
| MaeI | CTAG | 3 cut(s) | 494, 2048, 2060 |
| MalI | GATC | 9 cut(s) | 36, 81, 575, 713, 1946, 2043, 2121, 2175, 2223 |
| MboI | GATC | 9 cut(s) | 34, 79, 573, 711, 1944, 2041, 2119, 2173, 2221 |
| MflI | RGATCY | 2 cut(s) | 2173, 2221 |
| MhlI | GDGCHC | 5 cut(s) | 88, 222, 396, 1003, 1149 |
| MlsI | TGGCCA | 1 cut(s) | 206 |
| MluNI | TGGCCA | 1 cut(s) | 206 |
| MmeI | TCCRAC | 2 cut(s) | 369, 1197 |
| Mox20I | TGGCCA | 1 cut(s) | 206 |
| MroXI | GAANNNNTTC | 4 cut(s) | 291, 378, 471, 645 |
| MscI | TGGCCA | 1 cut(s) | 206 |
| MseI | TTAA | 3 cut(s) | 1206, 1461, 1629 |
| MslI | CAYNNNNRTG | 3 cut(s) | 1878, 1893, 2061 |
| Msp20I | TGGCCA | 1 cut(s) | 206 |
| MspI | CCGG | 3 cut(s) | 1965, 1984, 2171 |
| MspR9I | CCNGG | 3 cut(s) | 1264, 1544, 1965 |
| Mva1269I | GAATGC | 2 cut(s) | 328, 2000 |
| MvaI | CCWGG | 2 cut(s) | 1264, 1544 |
| MvnI | CGCG | 1 cut(s) | 1452 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 125, 1097, 1393, 1487, 1496, 1508 |
| NciI | CCSGG | 1 cut(s) | 1965 |
| NdeI | CATATG | 1 cut(s) | 12 |
| NdeII | GATC | 9 cut(s) | 34, 79, 573, 711, 1944, 2041, 2119, 2173, 2221 |
| NlaIII | CATG | 9 cut(s) | 62, 136, 863, 880, 1302, 1361, 1391, 1423, 1892 |
| NlaIV | GGNNCC | 5 cut(s) | 2163, 2175, 2211, 2235, 2259 |
| NmeAIII | GCCGAG | 2 cut(s) | 1162, 2101 |
| NspI | RCATGY | 3 cut(s) | 62, 136, 1361 |
| PaeR7I | CTCGAG | 4 cut(s) | 656, 1400, 1512, 2083 |
| PagI | TCATGA | 1 cut(s) | 1888 |
| PceI | AGGCCT | 2 cut(s) | 1469, 1562 |
| PciSI | GCTCTTC | 1 cut(s) | 1333 |
| PctI | GAATGC | 2 cut(s) | 328, 2000 |
| PdmI | GAANNNNTTC | 4 cut(s) | 291, 378, 471, 645 |
| PfeI | GAWTC | 7 cut(s) | 696, 704, 758, 928, 938, 1655, 1860 |
| PkrI | GCNGC | 7 cut(s) | 6, 138, 306, 1261, 1363, 1501, 1615 |
| PsiI | TTATAA | 1 cut(s) | 2126 |
| Psp124BI | GAGCTC | 2 cut(s) | 222, 396 |
| Psp6I | CCWGG | 2 cut(s) | 1262, 1542 |
| PspGI | CCWGG | 2 cut(s) | 1262, 1542 |
| PspN4I | GGNNCC | 5 cut(s) | 2163, 2175, 2211, 2235, 2259 |
| PspPI | GGNCC | 1 cut(s) | 903 |
| PspXI | VCTCGAGB | 1 cut(s) | 1512 |
| PstI | CTGCAG | 1 cut(s) | 1125 |
| PstNI | CAGNNNCTG | 1 cut(s) | 313 |
| PsuI | RGATCY | 2 cut(s) | 2173, 2221 |
| RsaI | GTAC | 3 cut(s) | 1247, 1956, 2137 |
| RsaNI | GTAC | 3 cut(s) | 1246, 1955, 2136 |
| RseI | CAYNNNNRTG | 3 cut(s) | 1878, 1893, 2061 |
| SacI | GAGCTC | 2 cut(s) | 222, 396 |
| SapI | GCTCTTC | 1 cut(s) | 1333 |
| SaqAI | TTAA | 3 cut(s) | 1206, 1461, 1629 |
| SatI | GCNGC | 7 cut(s) | 5, 137, 305, 1260, 1362, 1500, 1614 |
| Sau3AI | GATC | 9 cut(s) | 34, 79, 573, 711, 1944, 2041, 2119, 2173, 2221 |
| Sau96I | GGNCC | 1 cut(s) | 903 |
| ScaI | AGTACT | 1 cut(s) | 2137 |
| ScrFI | CCNGG | 3 cut(s) | 1264, 1544, 1965 |
| SduI | GDGCHC | 5 cut(s) | 88, 222, 396, 1003, 1149 |
| SfaNI | GCATC | 1 cut(s) | 415 |
| SfcI | CTRYAG | 2 cut(s) | 1121, 1378 |
| Sfr274I | CTCGAG | 4 cut(s) | 656, 1400, 1512, 2083 |
| SinI | GGWCC | 1 cut(s) | 903 |
| SlaI | CTCGAG | 4 cut(s) | 656, 1400, 1512, 2083 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 1878, 1893, 2061 |
| SmlI | CTYRAG | 7 cut(s) | 656, 986, 1150, 1400, 1512, 1847, 2083 |
| SmoI | CTYRAG | 7 cut(s) | 656, 986, 1150, 1400, 1512, 1847, 2083 |
| SseBI | AGGCCT | 2 cut(s) | 1469, 1562 |
| SsiI | CCGC | 4 cut(s) | 136, 1500, 1642, 1697 |
| SspI | AATATT | 1 cut(s) | 1486 |
| SspMI | CTAG | 3 cut(s) | 494, 2048, 2060 |
| SstI | GAGCTC | 2 cut(s) | 222, 396 |
| StuI | AGGCCT | 2 cut(s) | 1469, 1562 |
| StyD4I | CCNGG | 3 cut(s) | 1262, 1542, 1963 |
| StyI | CCWWGG | 3 cut(s) | 741, 1725, 1764 |
| TaaI | ACNGT | 2 cut(s) | 402, 1959 |
| TaqI | TCGA | 7 cut(s) | 82, 657, 1401, 1513, 1751, 1943, 2084 |
| TatI | WGTACW | 1 cut(s) | 2135 |
| TauI | GCSGC | 2 cut(s) | 139, 1502 |
| TfiI | GAWTC | 7 cut(s) | 696, 704, 758, 928, 938, 1655, 1860 |
| Tru1I | TTAA | 3 cut(s) | 1206, 1461, 1629 |
| Tru9I | TTAA | 3 cut(s) | 1206, 1461, 1629 |
| TscAI | CASTG | 4 cut(s) | 832, 1125, 1261, 2247 |
| TseI | GCWGC | 5 cut(s) | 4, 304, 1259, 1361, 1613 |
| TspGWI | ACGGA | 4 cut(s) | 521, 805, 1109, 1421 |
| TspRI | CASTG | 4 cut(s) | 832, 1125, 1261, 2247 |
| VneI | GTGCAC | 1 cut(s) | 999 |
| VpaK11BI | GGWCC | 1 cut(s) | 903 |
| XapI | RAATTY | 7 cut(s) | 291, 378, 471, 645, 763, 1522, 1567 |
| XbaI | TCTAGA | 2 cut(s) | 2047, 2059 |
| XceI | RCATGY | 3 cut(s) | 62, 136, 1361 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2060 |
| XhoI | CTCGAG | 4 cut(s) | 656, 1400, 1512, 2083 |
| XmnI | GAANNNNTTC | 4 cut(s) | 291, 378, 471, 645 |
| XspI | CTAG | 3 cut(s) | 494, 2048, 2060 |
| ZrmI | AGTACT | 1 cut(s) | 2137 |