Rmu_sc0004467.1_g000074

WEB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004467.1
OG0012986
Physical Location & Seq
Forward (+)
304184 .. 305365
1182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004467.1_g000074.1.cds

Sequence Viewer

Length: 1182 bp
atgaccaaggccgagttagagcactcaagggaacaagaacagcatttgaatgtaatgctaaagaccaatgaggaagattttaaattgatgttggatgaagtaaggaaagaagctgagaggcacaaaaacactgctgccaggttgaaaatagaacacgaagagtcggttatggcatggagtgggaaggaaactggctttgttgactgtattagaagagcagaagacgacagacatgctgcacagcaagagaactataggttgcatgagttgcttctcgagacggataacaaggccatgttgtcaaaggaagaaaatagtaaattgcgcgatatacttaaacaggccttgaatgaagcaaatatttccaaagaagctgcagtcattgctcgagctgaaaattctcagctcaaagatagcctggaagaaaaggaggaggccttgaattttctcacccgagaaaatgaaaattatagggtgaatgaaattgctgctaatgaaaacattaaggagttgaagcggttgcttattgaatcgtcaaagaaggactgcaagaaagaggaaaaagagaaaccgccattgaaggatgtgaaaaaggagtcgaaggataaaacacctacaaaggagtcgaagaaggaggctaaggagtcaaagcctacaaaggaggatagtgataaggagaaagaggctggaaacatacttggcaaagttttcagcttcagtctcaaggagcttagaatctcaaactcacacgatgatgttgttcatgttgttcaagatgatgatgatgatgatgatgagattgaaatagacgaggctctcaaaggctcgatctttgaggtacagtctcccggctccgctaatcatcaccggagaacttcttctgcattcacagattatggagaacccctccatccagaggattttgatcatctagaccatcatctagatgggactcacgccgagaactcgagaaagaagaaagccttgctaagaagatttggcgatcttataaagagaagtactgcttattcctactctaaaaaggagccatcaccggatcccaaaaaggaactattgcaggactcaaagaaggagccttcacaggatctcaaaaaggaaccttcactggatagcaagaaggaaccctcacaagatatcaagaaggaagcattaccagaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

393

Amino Acids

45.31

Weight (kDa)

5.51

Isoelectric Point (pI)

50.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012986)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G40020
fragaria_vesca FvH4_1g20290
malus_domestica MD03G1184100.v1.1 MD11G1200900.v1.1
prunus_persica Prupe.6G192500_v2.0.a1
pyrus_communis pycom03g13740
rosa_chinensis RchiOBHm_Chr2g0112431
rosa_laevigata RLG00000017980
rosa_multiflora Rmu_co8429047.1_g000001 Rmu_sc0004467.1_g000073 Rmu_sc0004467.1_g000074
rosa_roxburghii Rroxscaffold_2G00131450
rosa_rugosa Rorug02G0180500
rosa_samantha Rh2AG233900 Rh2BG247200 Rh2CG238400 Rh2DG241500
rosa_wichuraiana Rw2G018110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1010
AccII CGCG 1 cut(s) 327
AciI CCGC 3 cut(s) 517, 572, 855
AclWI GGATC 3 cut(s) 1052, 1065, 1113
AcsI RAATTY 2 cut(s) 397, 442
AcuI CTGAAG 1 cut(s) 700
AfaI GTAC 2 cut(s) 840, 1021
AfiI CCNNNNNNNGG 1 cut(s) 916
AgsI TTSAA 9 cut(s) 49, 145, 349, 442, 514, 530, 580, 773, 803
AjnI CCWGG 2 cut(s) 137, 417
AluBI AGCT 6 cut(s) 113, 374, 392, 406, 714, 730
AluI AGCT 6 cut(s) 113, 374, 392, 406, 714, 730
Alw21I GWGCWC 1 cut(s) 24
Alw26I GTCTC 3 cut(s) 272, 725, 849
AlwI GGATC 3 cut(s) 1052, 1065, 1113
Ama87I CYCGRG 4 cut(s) 275, 387, 453, 967
AoxI GGCC 4 cut(s) 9, 291, 342, 435
ApeKI GCWGC 4 cut(s) 134, 236, 374, 488
ApoI RAATTY 2 cut(s) 397, 442
Asp700I GAANNNNTTC 1 cut(s) 877
AspLEI GCGC 1 cut(s) 327
AsuC2I CCSGG 1 cut(s) 849
AsuHPI GGTGA 4 cut(s) 442, 487, 857, 1044
AvaI CYCGRG 4 cut(s) 275, 387, 453, 967
BamHI GGATCC 1 cut(s) 1057
BbsI GAAGAC 1 cut(s) 228
Bbv12I GWGCWC 1 cut(s) 24
BbvI GCAGC 4 cut(s) 121, 223, 361, 475
BccI CCATC 4 cut(s) 918, 941, 945, 1057
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 2 cut(s) 139, 419
BclI TGATCA 1 cut(s) 925
BcnI CCSGG 1 cut(s) 849
BcoDI GTCTC 3 cut(s) 272, 725, 849
BfaI CTAG 2 cut(s) 932, 944
BfmI CTRYAG 2 cut(s) 253, 375
BisI GCNGC 4 cut(s) 135, 237, 375, 489
BlsI GCNGC 4 cut(s) 136, 238, 376, 490
BmcAI AGTACT 1 cut(s) 1021
Bme1390I CCNGG 3 cut(s) 139, 419, 849
BmeT110I CYCGRG 4 cut(s) 275, 387, 453, 967
BmiI GGNNCC 6 cut(s) 853, 1047, 1059, 1095, 1119, 1143
BmrFI CCNGG 3 cut(s) 139, 419, 849
BpiI GAAGAC 1 cut(s) 228
BplI GAGNNNNNCTC 2 cut(s) 891, 923
Bpu10I CCTNAGC 1 cut(s) 639
BpuEI CTTGAG 2 cut(s) 10, 707
BpuMI CCSGG 1 cut(s) 849
BsaBI GATNNNNATC 1 cut(s) 924
BsaJI CCNNGG 1 cut(s) 6
BsaWI WCCGGW 2 cut(s) 867, 1054
Bsc4I CCNNNNNNNGG 1 cut(s) 916
Bse1I ACTGG 2 cut(s) 196, 1131
Bse3DI GCAATG 1 cut(s) 381
Bse8I GATNNNNATC 1 cut(s) 924
BseBI CCWGG 2 cut(s) 139, 419
BseDI CCNNGG 1 cut(s) 6
BseGI GGATG 3 cut(s) 100, 589, 910
BseJI GATNNNNATC 1 cut(s) 924
BseLI CCNNNNNNNGG 1 cut(s) 916
BseMI GCAATG 1 cut(s) 381
BseMII CTCAG 2 cut(s) 105, 416
BseNI ACTGG 2 cut(s) 196, 1131
BseRI GAGGAG 1 cut(s) 446
BseXI GCAGC 4 cut(s) 121, 223, 361, 475
BsgI GTGCAG 1 cut(s) 222
Bsh1236I CGCG 1 cut(s) 327
BshFI GGCC 4 cut(s) 11, 293, 344, 437
BsiHKAI GWGCWC 1 cut(s) 24
BsiHKCI CYCGRG 4 cut(s) 275, 387, 453, 967
BsiSI CCGG 3 cut(s) 849, 868, 1055
BslFI GGGAC 1 cut(s) 964
BslI CCNNNNNNNGG 1 cut(s) 916
BsmAI GTCTC 3 cut(s) 272, 725, 849
BsmBI CGTCTC 1 cut(s) 272
BsmFI GGGAC 1 cut(s) 964
BsmI GAATGC 1 cut(s) 884
BsnI GGCC 4 cut(s) 11, 293, 344, 437
BsoBI CYCGRG 4 cut(s) 275, 387, 453, 967
Bsp1286I GDGCHC 1 cut(s) 24
Bsp143I GATC 5 cut(s) 828, 925, 1003, 1057, 1105
BspACI CCGC 3 cut(s) 517, 572, 855
BspANI GGCC 4 cut(s) 11, 293, 344, 437
BspCNI CTCAG 2 cut(s) 106, 415
BspFNI CGCG 1 cut(s) 327
BspLI GGNNCC 6 cut(s) 853, 1047, 1059, 1095, 1119, 1143
BspMAI CTGCAG 1 cut(s) 379
BspPI GGATC 3 cut(s) 1052, 1065, 1113
BspQI GCTCTTC 1 cut(s) 208
BsrDI GCAATG 1 cut(s) 381
BsrI ACTGG 2 cut(s) 196, 1131
BssECI CCNNGG 1 cut(s) 6
BssMI GATC 5 cut(s) 828, 925, 1003, 1057, 1105
BssT1I CCWWGG 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 139, 419
Bst4CI ACNGT 2 cut(s) 206, 843
Bst6I CTCTTC 2 cut(s) 153, 208
BstAPI GCANNNNNTGC 2 cut(s) 268, 383
BstDEI CTNAG 5 cut(s) 114, 402, 639, 731, 989
BstF5I GGATG 3 cut(s) 100, 589, 910
BstFNI CGCG 1 cut(s) 327
BstHHI GCGC 1 cut(s) 327
BstKTI GATC 5 cut(s) 831, 928, 1006, 1060, 1108
BstMAI GTCTC 3 cut(s) 272, 725, 849
BstMBI GATC 5 cut(s) 828, 925, 1003, 1057, 1105
BstMWI GCNNNNNNNGC 2 cut(s) 268, 383
BstNI CCWGG 2 cut(s) 139, 419
BstNSI RCATGY 1 cut(s) 236
BstSCI CCNGG 3 cut(s) 137, 417, 847
BstSFI CTRYAG 2 cut(s) 253, 375
BstUI CGCG 1 cut(s) 327
BstV1I GCAGC 4 cut(s) 121, 223, 361, 475
BstV2I GAAGAC 1 cut(s) 228
BstX2I RGATCY 2 cut(s) 1057, 1105
BstYI RGATCY 2 cut(s) 1057, 1105
BsuRI GGCC 4 cut(s) 11, 293, 344, 437
BtsCI GGATG 3 cut(s) 100, 589, 910
BtsI GCAGTG 1 cut(s) 129
BtsIMutI CAGTG 2 cut(s) 129, 1124
CfoI GCGC 1 cut(s) 327
Csp6I GTAC 2 cut(s) 839, 1020
CviAII CATG 5 cut(s) 174, 233, 263, 295, 764
CviQI GTAC 2 cut(s) 839, 1020
DdeI CTNAG 5 cut(s) 114, 402, 639, 731, 989
DpnI GATC 5 cut(s) 830, 927, 1005, 1059, 1107
DpnII GATC 5 cut(s) 828, 925, 1003, 1057, 1105
DraI TTTAAA 1 cut(s) 82
Eam1104I CTCTTC 2 cut(s) 153, 208
EarI CTCTTC 2 cut(s) 153, 208
Eco130I CCWWGG 1 cut(s) 6
Eco147I AGGCCT 2 cut(s) 344, 437
Eco32I GATATC 1 cut(s) 1156
Eco57I CTGAAG 1 cut(s) 700
Eco88I CYCGRG 4 cut(s) 275, 387, 453, 967
EcoRII CCWGG 2 cut(s) 137, 417
EcoRV GATATC 1 cut(s) 1156
EcoT14I CCWWGG 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 272
FaeI CATG 5 cut(s) 177, 236, 266, 298, 767
FalI AAGNNNNNCTT 4 cut(s) 968, 1000, 1009, 1041
FaqI GGGAC 1 cut(s) 964
FatI CATG 5 cut(s) 173, 232, 262, 294, 763
FbaI TGATCA 1 cut(s) 925
Fnu4HI GCNGC 4 cut(s) 135, 237, 375, 489
FokI GGATG 3 cut(s) 107, 596, 897
Fsp4HI GCNGC 4 cut(s) 135, 237, 375, 489
FspBI CTAG 2 cut(s) 932, 944
GlaI GCGC 1 cut(s) 326
GluI GCNGC 4 cut(s) 135, 237, 375, 489
HaeIII GGCC 4 cut(s) 11, 293, 344, 437
HapII CCGG 3 cut(s) 849, 868, 1055
HhaI GCGC 1 cut(s) 327
Hin1II CATG 5 cut(s) 177, 236, 266, 298, 767
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 8 cut(s) 161, 530, 596, 623, 644, 735, 952, 1082
HpaII CCGG 3 cut(s) 849, 868, 1055
HphI GGTGA 4 cut(s) 442, 487, 857, 1044
Hpy166II GTNNAC 1 cut(s) 202
Hpy188III TCNNGA 8 cut(s) 275, 277, 773, 914, 932, 944, 969, 1159
Hpy8I GTNNAC 1 cut(s) 202
HpyCH4III ACNGT 2 cut(s) 206, 843
HpyCH4V TGCA 6 cut(s) 239, 262, 377, 549, 884, 1078
HpyF10VI GCNNNNNNNGC 2 cut(s) 268, 383
HpyF3I CTNAG 5 cut(s) 114, 402, 639, 731, 989
Hsp92II CATG 5 cut(s) 177, 236, 266, 298, 767
HspAI GCGC 1 cut(s) 325
Ksp22I TGATCA 1 cut(s) 925
Kzo9I GATC 5 cut(s) 828, 925, 1003, 1057, 1105
LguI GCTCTTC 1 cut(s) 208
LmnI GCTCC 4 cut(s) 727, 857, 1045, 1093
Lsp1109I GCAGC 4 cut(s) 121, 223, 361, 475
MaeI CTAG 2 cut(s) 932, 944
MalI GATC 5 cut(s) 830, 927, 1005, 1059, 1107
MboI GATC 5 cut(s) 828, 925, 1003, 1057, 1105
MflI RGATCY 2 cut(s) 1057, 1105
MhlI GDGCHC 1 cut(s) 24
MluCI AATT 6 cut(s) 83, 320, 397, 442, 466, 483
MlyI GAGTC 6 cut(s) 170, 605, 632, 653, 946, 1076
MmeI TCCRAC 1 cut(s) 72
MroXI GAANNNNTTC 1 cut(s) 877
MseI TTAA 3 cut(s) 81, 336, 504
MslI CAYNNNNRTG 3 cut(s) 48, 753, 945
MspI CCGG 3 cut(s) 849, 868, 1055
MspR9I CCNGG 3 cut(s) 139, 419, 849
Mva1269I GAATGC 1 cut(s) 884
MvaI CCWGG 2 cut(s) 139, 419
MvnI CGCG 1 cut(s) 327
MwoI GCNNNNNNNGC 2 cut(s) 268, 383
NciI CCSGG 1 cut(s) 849
NdeII GATC 5 cut(s) 828, 925, 1003, 1057, 1105
NlaIII CATG 5 cut(s) 177, 236, 266, 298, 767
NlaIV GGNNCC 6 cut(s) 853, 1047, 1059, 1095, 1119, 1143
NmeAIII GCCGAG 2 cut(s) 37, 985
NspI RCATGY 1 cut(s) 236
PaeR7I CTCGAG 3 cut(s) 275, 387, 967
PceI AGGCCT 2 cut(s) 344, 437
PciSI GCTCTTC 1 cut(s) 208
PctI GAATGC 1 cut(s) 884
PdmI GAANNNNTTC 1 cut(s) 877
PfeI GAWTC 2 cut(s) 530, 735
PkrI GCNGC 4 cut(s) 136, 238, 376, 490
PleI GAGTC 6 cut(s) 169, 604, 631, 652, 946, 1076
PpsI GAGTC 6 cut(s) 169, 604, 631, 652, 946, 1076
PsiI TTATAA 1 cut(s) 1010
Psp6I CCWGG 2 cut(s) 137, 417
PspGI CCWGG 2 cut(s) 137, 417
PspN4I GGNNCC 6 cut(s) 853, 1047, 1059, 1095, 1119, 1143
PspXI VCTCGAGB 1 cut(s) 387
PstI CTGCAG 1 cut(s) 379
PsuI RGATCY 2 cut(s) 1057, 1105
RsaI GTAC 2 cut(s) 840, 1021
RsaNI GTAC 2 cut(s) 839, 1020
RseI CAYNNNNRTG 3 cut(s) 48, 753, 945
SapI GCTCTTC 1 cut(s) 208
SaqAI TTAA 3 cut(s) 81, 336, 504
SatI GCNGC 4 cut(s) 135, 237, 375, 489
Sau3AI GATC 5 cut(s) 828, 925, 1003, 1057, 1105
ScaI AGTACT 1 cut(s) 1021
SchI GAGTC 6 cut(s) 170, 605, 632, 653, 946, 1076
ScrFI CCNGG 3 cut(s) 139, 419, 849
SduI GDGCHC 1 cut(s) 24
SfcI CTRYAG 2 cut(s) 253, 375
Sfr274I CTCGAG 3 cut(s) 275, 387, 967
SlaI CTCGAG 3 cut(s) 275, 387, 967
SmiMI CAYNNNNRTG 3 cut(s) 48, 753, 945
SmlI CTYRAG 5 cut(s) 25, 275, 387, 722, 967
SmoI CTYRAG 5 cut(s) 25, 275, 387, 722, 967
Sse9I AATT 6 cut(s) 83, 320, 397, 442, 466, 483
SseBI AGGCCT 2 cut(s) 344, 437
SsiI CCGC 3 cut(s) 517, 572, 855
SspI AATATT 1 cut(s) 361
SspMI CTAG 2 cut(s) 932, 944
StuI AGGCCT 2 cut(s) 344, 437
StyD4I CCNGG 3 cut(s) 137, 417, 847
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 2 cut(s) 206, 843
TaqI TCGA 6 cut(s) 276, 388, 599, 626, 827, 968
TasI AATT 6 cut(s) 83, 320, 397, 442, 466, 483
TatI WGTACW 1 cut(s) 1019
TfiI GAWTC 2 cut(s) 530, 735
Tru1I TTAA 3 cut(s) 81, 336, 504
Tru9I TTAA 3 cut(s) 81, 336, 504
TscAI CASTG 2 cut(s) 136, 1131
TseI GCWGC 4 cut(s) 134, 236, 374, 488
TspDTI ATGAA 6 cut(s) 111, 366, 477, 495, 510, 752
TspGWI ACGGA 1 cut(s) 296
TspRI CASTG 2 cut(s) 136, 1131
XapI RAATTY 2 cut(s) 397, 442
XbaI TCTAGA 2 cut(s) 931, 943
XceI RCATGY 1 cut(s) 236
XcmI CCANNNNNNNNNTGG 1 cut(s) 944
XhoI CTCGAG 3 cut(s) 275, 387, 967
XmnI GAANNNNTTC 1 cut(s) 877
XspI CTAG 2 cut(s) 932, 944
ZrmI AGTACT 1 cut(s) 1021
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.