Rh2AG233900

WEB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0012986
Physical Location & Seq
Forward (+)
25211808 .. 25218109
6302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG233900.1

Sequence Viewer

Length: 3531 bp
ATGATGTTGATACTCCTGGGGCTGATGATACTTTTGAGAACTACCGCCCGTTTGAACATCGTTTCATCCGTTTCAGGAGGTTGTTATTACGAACCCCAACGCTTGGTTCCTTCACTGCTCAAGCTTTTCTCAACCCATCATGTATACACTTGCAACGTCAAGATTGGCTCTCTCTTTCGAGCGGGTCATATTGAAGCTGCCCGCCAACTGTTCGACGAAATGCCCCAAAGAGACGTGGTTACATGGAACGCTCTCGTTACTGGGTACTGGAAAAACGGTTACTTTCAGGAATCAAAGAGTCTGTTCGAATCGATGCCTCTCAGGAATGTGGTGTCCTGGAACTCCATGGTAGCCGGGTGCGTCGAAAATGGGATGGTTGATGAGGCCTTCGGCTACTTTCGAGCCATGCCCGAGAGGAACATTGCTTCTTGGAATGCTATGATATCTGGGTTTTTGAAGTGTGATCGGGTGGAGGAAGCTAGTAGGCTTTTTGTGGAGATGCCGTGTAGGAATGTTGTTTCTTATACGGCGATGATTGATGGGTATGCGAAGAAAGGAGAGGTGGAGAAGGCAAGAGCATTGTTTGATGTCATGCCGCGTAAAAATGCGGTTTCTTGGACTGTGCTGATTAGTGTGTATGTGGAGAATGGGAGGTTTGATGAAGCTAGGGAATTGTACAAGCAGATGCCGGAGAAAAATGTGGTTGCAGTGACTGCCATGATCACTGGGTGCTGTAAGGAGGGTAAGATGGAAGATGCGAGAGTGTTGTTCGATGGAATGCAGTTCAAGGATGATGTCTGTTGGAATGCAATGATAACAGGCTATGCACAAAATGGTAGGGGAGAGGAAGCATTGAAGTTACATTCACAAAAGCTCAAGATTGGTTTGCATCCAGATAACTGGACCCTTGTTTCTGTTCTCATGGCCTGTTCTACTCTTGCGTCCCTGAAGGATGGAAGACAAGCTCATGTGCTCATTGTTAAACATGGTCTTGAATCCAATGTCTCCATAAGTAATGCTTTGATTACCATGTACTGCAGATGTGGTGCGATTCTTGACTCTGAGTTAGCTTTCAAACAAATTGAGAGTCCGGACCTTGTCTCATGGAACACCATTGTTGCTGCATTTGCACAGCACGAATTCTTCTTTGGTCATAATGCAAGACCAGTTCTGCATCAAAAAGAAGCCCTCTATCAATATATATGTGATTGTTGGAGCTTGGACATGCAGCAACATATGGAGCAACCTGAAGAGAAAGATCGCAAGATGAAAGAGCAATTGCATGCAGTAGAGGTAGAAAGAGATCGAGCCCTTGATGAACTAAGAGAGATGAAAAAGGTGGCACAGGAGGCTAACATGCGGCTTAGTGAAGCATTGTCAACTCATAATAAGGAGAATATAGACTCCTTGAAAGTTGAACTAGGAAAGGCCAAAGATTTGGAGCTCAAACTGGCAGAGAATGTGGAAAACGCTGAGGCTAAGACGAAGGTTTTAGTGTCTGAGAGCGAGGAACGAATTCAAGAATTGCAGCAGAAACTGACAGAGAATGCAAAAACCTCAGAGTCTCAGATGAAGGCTTTATTGTCCGAAAGAGAGGAAAGAATTCGGGAGTTAGAGCTCAAACTGTCAGAGAATGTGAAAACCTCCGATGCTCAGGCTGAAATAAAGACTTCATTGTCTGAAAGAGAAGAAAGAATTCGGGAATTGGAGCTCAAACTGGCAGAGAACACGGAAAGCTCAGAGGCTCACAGGAGGGCTTTATTGTCTGAAAGTGGAGAAAGAATAAGGAAATTGGAGATCAAATTGGCAGAGAATGTTGAAACCAATGAGGCTCAGTTGAAAACTTTGTTTTGTCAAAGTGAGGAAAGAATTCGGGAACTTGAGATGGAAATAGAAAAAAGAAGGAAATCAGAAGCAAGAATCCTTGATTCGTTGATCACACAGACAAACCAACTGGAGCAAACCAAGGTTTTACTTGAAGAATCAAATTCTGAAATTACTTCTCTTCAAAAGAAATTGGAGAAATGGGAAGAGGAGTCCGTGCAGAGCAGTGATCTTAGCACATCACATTTTCAGGACAGCCATGATACTAATGTTTCCATGAGGGATGATTTAGAGAGTCTCAGGTCCGAGCTTCAGTTGACAAAGGAGAATCTGGTTGATTCTCAAAAGAAGGAGCAACTTGCGTCTTTGAAGGTTGAGAAATTACTTGAGGAGATACGTGCACTAAAACACGAGCTGAAGTTGACAGCCGAAGCTGAAGAAAACGGAAAGAAGGCAATGGATGACTTGGCTATTGCTCTAAAAGAAGTTGCCACGGAAGCCAATCAGTTGAAGGACTCACTGCAGATGACCAAAGCCGAGTTAGAGCACTCAAGGGAACAAGAACAGCGTTTGAATGTAGTGCTAAAGACCAATGAGGAAGATTTTAAATTGATGTTGGATGAAGTAAGGAAAGAAGCTGAGAGGCACAAAAACACTGCTGCCAGGTTGAAAATAGAACACGAAGAGTCGGTTATGGCATGGAGTGGGAAGGAAACTGGCTTTGTTGACTGTATTAGAAGAGCAGAAGACGACAGACATGCTGCACAGCAAGAGAACTATAGGTTGCATGAGTTGCTTCTCGAGACGGATAACAAAGCCATGTTGTCAAAGGAAGAAAATAGTAAATTGCGCGATATACTTAAACAGGCCTTGAATGAAGCAAATATTGCCAAAGAAGCTGCAGTCATTGCTCGAGCTGAAAATTCTCAGCTCAAAGATAGCCTGGAAGAAAAGGAGGAGGCCTTGAATTTTCTCACCCGAGAAAATGAAAATTATAGGGTGAATGAAATTGCTGCTAATGAAAACATTAAGGAGTTGAAGCGGTTGCTTATTGAATCGTCAAAGAAGGACAGCAAGAAAGAGGAAAAAGAGAAACCGCCATTGAAGGATGTGAAAAAGGAGTCCAAGGATAAAACACCTACAAAGGAATCGAAGAAGGAGGCTAAGGAGTCAAAGCCTACAAAGGAGGATAGTGATAAGGAGAAAGAGGCTGGAAACATACTTGGCAAAGTTTTCAGCTTCAGTCTCAAGGAGCTTAGAATCTCAAACTCACACGATGATGTTGTTCATGTTGTTCAAGATGATGATGATGATGATGATGAGATTGAAATAGATGAGGCTCTCAAAGGCTCGATCTTTGAGGTACAGTCTCCCGGCTCCGCTAATCATCACCGGAGAACTTCTTCTGCATTCACAGATTATGGAGAACCCCTCCATCCAGAGGATTTTGATCATCTAGACCATCATCTAGATGGGACTCACGCCGAGAACTCGAGAAAGAAGAAAGCCTTGCTAAGAAGATTTGGTGATCTTATAAAGAGAAGTACTGCTTATTCCTACTCTAAAAAGGAGCCATCACCGGATCCCAAAAAGGAACTATTGCAGGACGCAAAGAAGGAGCCTTCACAGGATCTCAAAAAGGAACCTTCACTGGATAGCAAGAAGGAACCCTCACAAGATATCAAGAAGGAAGCATTACCAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1176

Amino Acids

134.66

Weight (kDa)

5.44

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 108 - 141 9.5e-06 PPR repeat family
PPR PF01535 111 - 141 6.4e-07 PPR repeat
PPR_2 PF13041 140 - 172 9.7e-07 PPR repeat family
PPR PF01535 143 - 171 2.5e-06 PPR repeat
PPR_2 PF13041 170 - 202 1e-07 PPR repeat family
PPR_1 PF12854 170 - 198 1.4e-07 PPR repeat
PPR PF01535 173 - 202 1.1e-07 PPR repeat
PPR_2 PF13041 201 - 234 1.6e-06 PPR repeat family
PPR PF01535 204 - 234 8.2e-08 PPR repeat
PPR_2 PF13041 264 - 310 2.8e-07 PPR repeat family
PPR PF01535 266 - 290 4e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012986)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G40020
fragaria_vesca FvH4_1g20290
malus_domestica MD03G1184100.v1.1 MD11G1200900.v1.1
prunus_persica Prupe.6G192500_v2.0.a1
pyrus_communis pycom03g13740
rosa_chinensis RchiOBHm_Chr2g0112431
rosa_laevigata RLG00000017980
rosa_multiflora Rmu_co8429047.1_g000001 Rmu_sc0004467.1_g000073 Rmu_sc0004467.1_g000074
rosa_roxburghii Rroxscaffold_2G00131450
rosa_rugosa Rorug02G0180500
rosa_samantha Rh2AG233900 Rh2BG247200 Rh2CG238400 Rh2DG241500
rosa_wichuraiana Rw2G018110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3359
AasI GACNNNNNNGTC 1 cut(s) 1675
AccB7I CCANNNNNTGG 1 cut(s) 103
AccBSI CCGCTC 1 cut(s) 182
AccI GTMKAC 1 cut(s) 144
AccII CGCG 2 cut(s) 598, 2676
AccIII TCCGGA 1 cut(s) 1090
AciI CCGC 9 cut(s) 45, 182, 202, 596, 608, 1360, 2866, 2921, 3204
AclWI GGATC 3 cut(s) 3401, 3414, 3462
AcsI RAATTY 8 cut(s) 1139, 1515, 1602, 1695, 1869, 1987, 2746, 2791
AcuI CTGAAG 6 cut(s) 968, 1269, 2120, 2261, 2280, 3049
AdeI CACNNNGTG 1 cut(s) 729
AfaI GTAC 5 cut(s) 266, 677, 1034, 3189, 3370
AfiI CCNNNNNNNGG 2 cut(s) 103, 3265
AjiI CACGTC 1 cut(s) 235
AjnI CCWGG 4 cut(s) 15, 335, 2486, 2766
AjuI GAANNNNNNNTTGG 4 cut(s) 90, 122, 1131, 1163
Alw21I GWGCWC 6 cut(s) 975, 1446, 1620, 1713, 2227, 2373
Alw26I GTCTC 8 cut(s) 225, 1009, 1105, 1569, 2126, 2621, 3074, 3198
Alw44I GTGCAC 1 cut(s) 2223
AlwI GGATC 3 cut(s) 3401, 3414, 3462
AlwNI CAGNNNCTG 2 cut(s) 713, 1537
Ama87I CYCGRG 5 cut(s) 410, 2624, 2736, 2802, 3316
Aor13HI TCCGGA 1 cut(s) 1090
AoxI GGCC 5 cut(s) 384, 924, 1428, 2691, 2784
ApaLI GTGCAC 1 cut(s) 2223
ApeKI GCWGC 8 cut(s) 197, 1121, 1228, 1528, 2483, 2585, 2723, 2837
ApoI RAATTY 8 cut(s) 1139, 1515, 1602, 1695, 1869, 1987, 2746, 2791
ArsI GACNNNNNNTTYG 2 cut(s) 1072, 1104
Asp700I GAANNNNTTC 5 cut(s) 1515, 1602, 1695, 1869, 3226
AspLEI GCGC 1 cut(s) 2676
AspS9I GGNCC 3 cut(s) 903, 1093, 2127
AsuC2I CCSGG 2 cut(s) 355, 3198
AsuHPI GGTGA 5 cut(s) 2791, 2836, 3206, 3362, 3393
AsuII TTCGAA 1 cut(s) 306
AvaI CYCGRG 5 cut(s) 410, 2624, 2736, 2802, 3316
AvaII GGWCC 3 cut(s) 903, 1093, 2127
BaeGI GKGCMC 1 cut(s) 2227
BaeI ACNNNNGTAYC 2 cut(s) 2079, 2112
BamHI GGATCC 1 cut(s) 3406
BanII GRGCYC 4 cut(s) 1312, 1446, 1620, 1713
BauI CACGAG 1 cut(s) 2234
BbsI GAAGAC 2 cut(s) 964, 2577
Bbv12I GWGCWC 6 cut(s) 975, 1446, 1620, 1713, 2227, 2373
BbvCI CCTCAGC 1 cut(s) 1473
BbvI GCAGC 8 cut(s) 184, 1108, 1240, 1540, 2470, 2572, 2710, 2824
BceAI ACGGC 2 cut(s) 487, 543
BcgI CGANNNNNNTGC 2 cut(s) 2564, 2598
BciT130I CCWGG 4 cut(s) 17, 337, 2488, 2768
BclI TGATCA 3 cut(s) 720, 1935, 3274
BcnI CCSGG 2 cut(s) 355, 3198
BcoDI GTCTC 8 cut(s) 225, 1009, 1105, 1569, 2126, 2621, 3074, 3198
BfaI CTAG 5 cut(s) 480, 666, 1421, 3281, 3293
BfmI CTRYAG 4 cut(s) 1036, 2345, 2602, 2724
BmcAI AGTACT 1 cut(s) 3370
Bme1390I CCNGG 6 cut(s) 17, 337, 355, 2488, 2768, 3198
Bme18I GGWCC 3 cut(s) 903, 1093, 2127
BmeT110I CYCGRG 5 cut(s) 410, 2624, 2736, 2802, 3316
BmgBI CACGTC 1 cut(s) 235
BmgT120I GGNCC 3 cut(s) 903, 1093, 2127
BmiI GGNNCC 8 cut(s) 108, 905, 3202, 3396, 3408, 3444, 3468, 3492
BmrFI CCNGG 6 cut(s) 17, 337, 355, 2488, 2768, 3198
BmrI ACTGGG 2 cut(s) 270, 735
BmsI GCATC 7 cut(s) 303, 489, 675, 745, 898, 1183, 1639
BmuI ACTGGG 2 cut(s) 270, 735
BpiI GAAGAC 2 cut(s) 964, 2577
BplI GAGNNNNNCTC 2 cut(s) 3240, 3272
BpmI CTGGAG 1 cut(s) 1976
Bpu10I CCTNAGC 3 cut(s) 1473, 1653, 2988
Bpu14I TTCGAA 1 cut(s) 306
BpuEI CTTGAG 6 cut(s) 104, 860, 1901, 2231, 2359, 3056
BpuMI CCSGG 2 cut(s) 355, 3198
Bsa29I ATCGAT 1 cut(s) 311
BsaAI YACGTR 1 cut(s) 2222
BsaBI GATNNNNATC 1 cut(s) 3273
BsaJI CCNNGG 5 cut(s) 16, 345, 1965, 2316, 2949
BsaWI WCCGGW 3 cut(s) 1090, 3216, 3403
BsaXI ACNNNNNCTCC 2 cut(s) 488, 518
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 3265
Bse3DI GCAATG 4 cut(s) 420, 816, 2286, 2730
Bse8I GATNNNNATC 1 cut(s) 3273
BseAI TCCGGA 1 cut(s) 1090
BseBI CCWGG 4 cut(s) 17, 337, 2488, 2768
BseCI ATCGAT 1 cut(s) 311
BseDI CCNNGG 5 cut(s) 16, 345, 1965, 2316, 2949
BseJI GATNNNNATC 1 cut(s) 3273
BseLI CCNNNNNNNGG 2 cut(s) 103, 3265
BseMI GCAATG 4 cut(s) 420, 816, 2286, 2730
BseRI GAGGAG 3 cut(s) 2048, 2228, 2795
BseSI GKGCMC 1 cut(s) 2227
BseXI GCAGC 8 cut(s) 184, 1108, 1240, 1540, 2470, 2572, 2710, 2824
BsgI GTGCAG 2 cut(s) 2063, 2571
Bsh1236I CGCG 2 cut(s) 598, 2676
BshFI GGCC 5 cut(s) 386, 926, 1430, 2693, 2786
BshVI ATCGAT 1 cut(s) 311
BsiHKAI GWGCWC 6 cut(s) 975, 1446, 1620, 1713, 2227, 2373
BsiHKCI CYCGRG 5 cut(s) 410, 2624, 2736, 2802, 3316
BsiSI CCGG 6 cut(s) 354, 689, 1091, 3198, 3217, 3404
BslFI GGGAC 2 cut(s) 928, 3313
BslI CCNNNNNNNGG 2 cut(s) 103, 3265
BsmAI GTCTC 8 cut(s) 225, 1009, 1105, 1569, 2126, 2621, 3074, 3198
BsmBI CGTCTC 2 cut(s) 225, 2621
BsmFI GGGAC 2 cut(s) 928, 3313
BsmI GAATGC 5 cut(s) 439, 783, 811, 1552, 3233
BsnI GGCC 5 cut(s) 386, 926, 1430, 2693, 2786
BsoBI CYCGRG 5 cut(s) 410, 2624, 2736, 2802, 3316
Bsp119I TTCGAA 1 cut(s) 306
Bsp1286I GDGCHC 7 cut(s) 975, 1312, 1446, 1620, 1713, 2227, 2373
Bsp13I TCCGGA 1 cut(s) 1090
Bsp1407I TGTACA 1 cut(s) 675
Bsp19I CCATGG 1 cut(s) 345
BspACI CCGC 9 cut(s) 45, 182, 202, 596, 608, 1360, 2866, 2921, 3204
BspANI GGCC 5 cut(s) 386, 926, 1430, 2693, 2786
BspDI ATCGAT 1 cut(s) 311
BspEI TCCGGA 1 cut(s) 1090
BspFNI CGCG 2 cut(s) 598, 2676
BspLI GGNNCC 8 cut(s) 108, 905, 3202, 3396, 3408, 3444, 3468, 3492
BspMAI CTGCAG 3 cut(s) 1040, 2349, 2728
BspPI GGATC 3 cut(s) 3401, 3414, 3462
BspQI GCTCTTC 1 cut(s) 2557
BspT104I TTCGAA 1 cut(s) 306
BsrBI CCGCTC 1 cut(s) 182
BsrDI GCAATG 4 cut(s) 420, 816, 2286, 2730
BsrGI TGTACA 1 cut(s) 675
BssECI CCNNGG 5 cut(s) 16, 345, 1965, 2316, 2949
BssNAI GTATAC 1 cut(s) 145
BssSI CACGAG 1 cut(s) 2234
BssT1I CCWWGG 3 cut(s) 345, 1965, 2949
Bst1107I GTATAC 1 cut(s) 145
Bst2BI CACGAG 1 cut(s) 2234
Bst2UI CCWGG 4 cut(s) 17, 337, 2488, 2768
Bst4CI ACNGT 6 cut(s) 210, 278, 622, 1626, 2555, 3192
Bst6I CTCTTC 5 cut(s) 1245, 2010, 2025, 2502, 2557
BstAPI GCANNNNNTGC 4 cut(s) 713, 2617, 2711, 2732
BstAUI TGTACA 1 cut(s) 675
BstBAI YACGTR 1 cut(s) 2222
BstBI TTCGAA 1 cut(s) 306
BstC8I GCNNGC 2 cut(s) 202, 1284
BstDSI CCRYGG 2 cut(s) 345, 2316
BstFNI CGCG 2 cut(s) 598, 2676
BstHHI GCGC 1 cut(s) 2676
BstMAI GTCTC 8 cut(s) 225, 1009, 1105, 1569, 2126, 2621, 3074, 3198
BstMWI GCNNNNNNNGC 8 cut(s) 713, 1127, 1349, 2321, 2617, 2711, 2720, 2732
BstNI CCWGG 4 cut(s) 17, 337, 2488, 2768
BstNSI RCATGY 4 cut(s) 1228, 1286, 1360, 2585
BstSCI CCNGG 6 cut(s) 15, 335, 353, 2486, 2766, 3196
BstSFI CTRYAG 4 cut(s) 1036, 2345, 2602, 2724
BstSLI GKGCMC 1 cut(s) 2227
BstUI CGCG 2 cut(s) 598, 2676
BstV1I GCAGC 8 cut(s) 184, 1108, 1240, 1540, 2470, 2572, 2710, 2824
BstV2I GAAGAC 2 cut(s) 964, 2577
BstX2I RGATCY 2 cut(s) 3406, 3454
BstXI CCANNNNNNTGG 2 cut(s) 900, 1438
BstYI RGATCY 2 cut(s) 3406, 3454
BstZ17I GTATAC 1 cut(s) 145
Bsu15I ATCGAT 1 cut(s) 311
BsuRI GGCC 5 cut(s) 386, 926, 1430, 2693, 2786
BsuTUI ATCGAT 1 cut(s) 311
BtgI CCRYGG 2 cut(s) 345, 2316
BtgZI GCGATG 1 cut(s) 545
BtrI CACGTC 1 cut(s) 235
BtsI GCAGTG 5 cut(s) 113, 714, 2056, 2342, 2478
BtsIMutI CAGTG 7 cut(s) 113, 714, 723, 2056, 2342, 2478, 3473
Cac8I GCNNGC 2 cut(s) 202, 1284
CaiI CAGNNNCTG 2 cut(s) 713, 1537
CfoI GCGC 1 cut(s) 2676
Cfr13I GGNCC 3 cut(s) 903, 1093, 2127
ClaI ATCGAT 1 cut(s) 311
CseI GACGC 4 cut(s) 349, 930, 2175, 3440
Csp6I GTAC 5 cut(s) 265, 676, 1033, 3188, 3369
CviQI GTAC 5 cut(s) 265, 676, 1033, 3188, 3369
DraI TTTAAA 1 cut(s) 2431
DraIII CACNNNGTG 1 cut(s) 729
DrdI GACNNNNNNGTC 1 cut(s) 1675
DseDI GACNNNNNNGTC 1 cut(s) 1675
Eam1104I CTCTTC 5 cut(s) 1245, 2010, 2025, 2502, 2557
EarI CTCTTC 5 cut(s) 1245, 2010, 2025, 2502, 2557
Ecl136II GAGCTC 3 cut(s) 1444, 1618, 1711
Eco130I CCWWGG 3 cut(s) 345, 1965, 2949
Eco147I AGGCCT 3 cut(s) 386, 2693, 2786
Eco24I GRGCYC 4 cut(s) 1312, 1446, 1620, 1713
Eco32I GATATC 2 cut(s) 444, 3505
Eco47I GGWCC 3 cut(s) 903, 1093, 2127
Eco53kI GAGCTC 3 cut(s) 1444, 1618, 1711
Eco57I CTGAAG 6 cut(s) 968, 1269, 2120, 2261, 2280, 3049
Eco88I CYCGRG 5 cut(s) 410, 2624, 2736, 2802, 3316
EcoICRI GAGCTC 3 cut(s) 1444, 1618, 1711
EcoRI GAATTC 5 cut(s) 1139, 1515, 1602, 1695, 1869
EcoRII CCWGG 4 cut(s) 15, 335, 2486, 2766
EcoRV GATATC 2 cut(s) 444, 3505
EcoT14I CCWWGG 3 cut(s) 345, 1965, 2949
EcoT38I GRGCYC 4 cut(s) 1312, 1446, 1620, 1713
ErhI CCWWGG 3 cut(s) 345, 1965, 2949
Esp3I CGTCTC 2 cut(s) 225, 2621
FalI AAGNNNNNCTT 6 cut(s) 1003, 1035, 3317, 3349, 3358, 3390
FaqI GGGAC 2 cut(s) 928, 3313
FauI CCCGC 2 cut(s) 175, 209
FauNDI CATATG 1 cut(s) 1236
FbaI TGATCA 3 cut(s) 720, 1935, 3274
FblI GTMKAC 1 cut(s) 144
FriOI GRGCYC 4 cut(s) 1312, 1446, 1620, 1713
FspBI CTAG 5 cut(s) 480, 666, 1421, 3281, 3293
GlaI GCGC 1 cut(s) 2675
GsuI CTGGAG 1 cut(s) 1976
HaeIII GGCC 5 cut(s) 386, 926, 1430, 2693, 2786
HapII CCGG 6 cut(s) 354, 689, 1091, 3198, 3217, 3404
HgaI GACGC 4 cut(s) 349, 930, 2175, 3440
HhaI GCGC 1 cut(s) 2676
Hin6I GCGC 1 cut(s) 2674
HinP1I GCGC 1 cut(s) 2674
HincII GTYRAC 4 cut(s) 1380, 2142, 2247, 2551
HindII GTYRAC 4 cut(s) 1380, 2142, 2247, 2551
HindIII AAGCTT 1 cut(s) 122
HpaII CCGG 6 cut(s) 354, 689, 1091, 3198, 3217, 3404
HphI GGTGA 5 cut(s) 2791, 2836, 3206, 3362, 3393
Hpy166II GTNNAC 6 cut(s) 145, 1380, 2142, 2225, 2247, 2551
Hpy8I GTNNAC 6 cut(s) 145, 1380, 2142, 2225, 2247, 2551
Hpy99I CGWCG 2 cut(s) 218, 365
HpyCH4III ACNGT 6 cut(s) 210, 278, 622, 1626, 2555, 3192
HpyCH4IV ACGT 3 cut(s) 156, 234, 2221
HpyF10VI GCNNNNNNNGC 8 cut(s) 713, 1127, 1349, 2321, 2617, 2711, 2720, 2732
HpySE526I ACGT 3 cut(s) 156, 234, 2221
HspAI GCGC 1 cut(s) 2674
Kpn2I TCCGGA 1 cut(s) 1090
Ksp22I TGATCA 3 cut(s) 720, 1935, 3274
LguI GCTCTTC 1 cut(s) 2557
Lsp1109I GCAGC 8 cut(s) 184, 1108, 1240, 1540, 2470, 2572, 2710, 2824
LweI GCATC 7 cut(s) 303, 489, 675, 745, 898, 1183, 1639
MaeI CTAG 5 cut(s) 480, 666, 1421, 3281, 3293
MaeII ACGT 3 cut(s) 156, 234, 2221
MaeIII GTNAC 5 cut(s) 238, 256, 278, 709, 858
MbiI CCGCTC 1 cut(s) 182
MfeI CAATTG 1 cut(s) 1277
MflI RGATCY 2 cut(s) 3406, 3454
MhlI GDGCHC 7 cut(s) 975, 1312, 1446, 1620, 1713, 2227, 2373
MmeI TCCRAC 3 cut(s) 782, 1193, 2421
MroI TCCGGA 1 cut(s) 1090
MroXI GAANNNNTTC 5 cut(s) 1515, 1602, 1695, 1869, 3226
MseI TTAA 4 cut(s) 981, 2430, 2685, 2853
MslI CAYNNNNRTG 2 cut(s) 3102, 3294
MspI CCGG 6 cut(s) 354, 689, 1091, 3198, 3217, 3404
MspR9I CCNGG 6 cut(s) 17, 337, 355, 2488, 2768, 3198
MunI CAATTG 1 cut(s) 1277
Mva1269I GAATGC 5 cut(s) 439, 783, 811, 1552, 3233
MvaI CCWGG 4 cut(s) 17, 337, 2488, 2768
MvnI CGCG 2 cut(s) 598, 2676
MwoI GCNNNNNNNGC 8 cut(s) 713, 1127, 1349, 2321, 2617, 2711, 2720, 2732
NciI CCSGG 2 cut(s) 355, 3198
NcoI CCATGG 1 cut(s) 345
NdeI CATATG 1 cut(s) 1236
NlaIV GGNNCC 8 cut(s) 108, 905, 3202, 3396, 3408, 3444, 3468, 3492
NmeAIII GCCGAG 2 cut(s) 2386, 3334
NmuCI GTSAC 1 cut(s) 709
NspI RCATGY 4 cut(s) 1228, 1286, 1360, 2585
NspV TTCGAA 1 cut(s) 306
PaeI GCATGC 1 cut(s) 1286
PaeR7I CTCGAG 3 cut(s) 2624, 2736, 3316
PceI AGGCCT 3 cut(s) 386, 2693, 2786
PciSI GCTCTTC 1 cut(s) 2557
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 5 cut(s) 439, 783, 811, 1552, 3233
PdmI GAANNNNTTC 5 cut(s) 1515, 1602, 1695, 1869, 3226
PflFI GACNNNGTC 1 cut(s) 1097
PflMI CCANNNNNTGG 1 cut(s) 103
PfoI TCCNGGA 1 cut(s) 335
Ppu21I YACGTR 1 cut(s) 2222
PsiI TTATAA 1 cut(s) 3359
Psp124BI GAGCTC 3 cut(s) 1446, 1620, 1713
Psp6I CCWGG 4 cut(s) 15, 335, 2486, 2766
PspGI CCWGG 4 cut(s) 15, 335, 2486, 2766
PspN4I GGNNCC 8 cut(s) 108, 905, 3202, 3396, 3408, 3444, 3468, 3492
PspPI GGNCC 3 cut(s) 903, 1093, 2127
PspXI VCTCGAGB 1 cut(s) 2736
PstI CTGCAG 3 cut(s) 1040, 2349, 2728
PstNI CAGNNNCTG 2 cut(s) 713, 1537
PsuI RGATCY 2 cut(s) 3406, 3454
PsyI GACNNNGTC 1 cut(s) 1097
RsaI GTAC 5 cut(s) 266, 677, 1034, 3189, 3370
RsaNI GTAC 5 cut(s) 265, 676, 1033, 3188, 3369
RseI CAYNNNNRTG 2 cut(s) 3102, 3294
SacI GAGCTC 3 cut(s) 1446, 1620, 1713
SapI GCTCTTC 1 cut(s) 2557
SaqAI TTAA 4 cut(s) 981, 2430, 2685, 2853
Sau96I GGNCC 3 cut(s) 903, 1093, 2127
ScaI AGTACT 1 cut(s) 3370
ScrFI CCNGG 6 cut(s) 17, 337, 355, 2488, 2768, 3198
SduI GDGCHC 7 cut(s) 975, 1312, 1446, 1620, 1713, 2227, 2373
SfaNI GCATC 7 cut(s) 303, 489, 675, 745, 898, 1183, 1639
SfcI CTRYAG 4 cut(s) 1036, 2345, 2602, 2724
Sfr274I CTCGAG 3 cut(s) 2624, 2736, 3316
SfuI TTCGAA 1 cut(s) 306
SinI GGWCC 3 cut(s) 903, 1093, 2127
SlaI CTCGAG 3 cut(s) 2624, 2736, 3316
SmiMI CAYNNNNRTG 2 cut(s) 3102, 3294
SmlI CTYRAG 9 cut(s) 119, 875, 1880, 2210, 2374, 2624, 2736, 3071, 3316
SmoI CTYRAG 9 cut(s) 119, 875, 1880, 2210, 2374, 2624, 2736, 3071, 3316
SphI GCATGC 1 cut(s) 1286
SseBI AGGCCT 3 cut(s) 386, 2693, 2786
SsiI CCGC 9 cut(s) 45, 182, 202, 596, 608, 1360, 2866, 2921, 3204
SspI AATATT 1 cut(s) 2710
SspMI CTAG 5 cut(s) 480, 666, 1421, 3281, 3293
SstI GAGCTC 3 cut(s) 1446, 1620, 1713
StuI AGGCCT 3 cut(s) 386, 2693, 2786
StyD4I CCNGG 6 cut(s) 15, 335, 353, 2486, 2766, 3196
StyI CCWWGG 3 cut(s) 345, 1965, 2949
TaaI ACNGT 6 cut(s) 210, 278, 622, 1626, 2555, 3192
TaiI ACGT 3 cut(s) 159, 237, 2224
TatI WGTACW 3 cut(s) 675, 1032, 3368
TauI GCSGC 2 cut(s) 598, 1363
Tru1I TTAA 4 cut(s) 981, 2430, 2685, 2853
Tru9I TTAA 4 cut(s) 981, 2430, 2685, 2853
TscAI CASTG 7 cut(s) 120, 714, 730, 2056, 2349, 2485, 3480
TseFI GTSAC 1 cut(s) 709
TseI GCWGC 8 cut(s) 197, 1121, 1228, 1528, 2483, 2585, 2723, 2837
Tsp45I GTSAC 1 cut(s) 709
TspGWI ACGGA 6 cut(s) 58, 1745, 2029, 2283, 2333, 2645
TspRI CASTG 7 cut(s) 120, 714, 730, 2056, 2349, 2485, 3480
Tth111I GACNNNGTC 1 cut(s) 1097
Van91I CCANNNNNTGG 1 cut(s) 103
VneI GTGCAC 1 cut(s) 2223
VpaK11BI GGWCC 3 cut(s) 903, 1093, 2127
XapI RAATTY 8 cut(s) 1139, 1515, 1602, 1695, 1869, 1987, 2746, 2791
XbaI TCTAGA 2 cut(s) 3280, 3292
XceI RCATGY 4 cut(s) 1228, 1286, 1360, 2585
XcmI CCANNNNNNNNNTGG 1 cut(s) 3293
XhoI CTCGAG 3 cut(s) 2624, 2736, 3316
XmiI GTMKAC 1 cut(s) 144
XmnI GAANNNNTTC 5 cut(s) 1515, 1602, 1695, 1869, 3226
XspI CTAG 5 cut(s) 480, 666, 1421, 3281, 3293
ZrmI AGTACT 1 cut(s) 3370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.