RLG00000018456

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018952
Physical Location & Seq
Reverse (-)
29989270 .. 29993347
4078 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018456

Sequence Viewer

Length: 516 bp
ATGCTTAAGCTTCGAAACCCTAGATTAAAGGTGGAGGGAGAGGAGGTCGGAGTACTTGGATTGGCAGAGTTGGGCGGCGAGGACGGAGTTGACAGTGTAGATGGTGCGGTGGCAGTTGGAGTAGATGGAGTGGCGGGTGTAGAGGGCAGCTTCTTCGTTGCGGCAGATGAAGAGCTTACAAGTCTTCTATGCCATAAGAGGTTGAGGAAGACGATGAAGAGTACAACTCCGCCACTATCGAACTCATTGAGCCACGACTCAGAGTCTCAGATCGACCACGGCCTCTTCCTCTTGTCGCAGATCGAGGTTGCCTCCAGCTCTAAGCTCCATGCCTCATCCTCTTCTCTCAGATCGACCACGACCTCTTCCTCTTGTCTCAGATCGAGGTTGCGTCCAGCTCCAAGCTCCATCCCTCATCCTCTTCTCTCAGATAGACCACGACCTCTTCCGCATCCGATCAATTTCGTCTCTGATTTTGGGTTCTACCAGGTCATTTTCTTCTCCCCTCAGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

172

Amino Acids

18.36

Weight (kDa)

6.05

Isoelectric Point (pI)

56.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018952)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0320341
rosa_laevigata RLG00000018456 RLG00000023116
rosa_multiflora Rmu_sc0004125.1_g000011
rosa_roxburghii Rroxscaffold_7G00192370
rosa_rugosa Rorug01G0346200
rosa_samantha Rh1AG448300 Rh1CG418000 Rh6DG320200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 75, 107, 134, 161, 230, 449
AfaI GTAC 2 cut(s) 54, 223
AflII CTTAAG 1 cut(s) 5
AhdI GACNNNNNGTC 1 cut(s) 262
AjnI CCWGG 1 cut(s) 486
AluBI AGCT 7 cut(s) 10, 150, 175, 318, 325, 398, 405
AluI AGCT 7 cut(s) 10, 150, 175, 318, 325, 398, 405
Alw26I GTCTC 3 cut(s) 270, 380, 472
AoxI GGCC 1 cut(s) 280
ApeKI GCWGC 1 cut(s) 147
AsuII TTCGAA 1 cut(s) 13
BbsI GAAGAC 2 cut(s) 176, 215
BbvI GCAGC 1 cut(s) 159
BccI CCATC 3 cut(s) 95, 119, 416
BceAI ACGGC 1 cut(s) 295
BcgI CGANNNNNNTGC 2 cut(s) 136, 170
BciT130I CCWGG 1 cut(s) 488
BcoDI GTCTC 3 cut(s) 270, 380, 472
BfaI CTAG 1 cut(s) 21
BfrI CTTAAG 1 cut(s) 5
BisI GCNGC 3 cut(s) 76, 148, 162
BlsI GCNGC 3 cut(s) 77, 149, 163
BmcAI AGTACT 1 cut(s) 54
Bme1390I CCNGG 1 cut(s) 488
BmeRI GACNNNNNGTC 1 cut(s) 262
BmrFI CCNGG 1 cut(s) 488
BmsI GCATC 1 cut(s) 460
BpiI GAAGAC 2 cut(s) 176, 215
BplI GAGNNNNNCTC 4 cut(s) 211, 243, 296, 328
BpmI CTGGAG 1 cut(s) 298
Bpu14I TTCGAA 1 cut(s) 13
BsaJI CCNNGG 1 cut(s) 277
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
BseBI CCWGG 1 cut(s) 488
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 4 cut(s) 335, 408, 415, 451
BseMII CTCAG 5 cut(s) 273, 281, 361, 391, 441
BseRI GAGGAG 1 cut(s) 56
BseXI GCAGC 1 cut(s) 159
BshFI GGCC 1 cut(s) 282
BsmAI GTCTC 3 cut(s) 270, 380, 472
BsmBI CGTCTC 1 cut(s) 472
BsnI GGCC 1 cut(s) 282
Bsp119I TTCGAA 1 cut(s) 13
Bsp143I GATC 5 cut(s) 270, 300, 350, 380, 456
BspACI CCGC 6 cut(s) 75, 107, 134, 161, 230, 449
BspANI GGCC 1 cut(s) 282
BspCNI CTCAG 5 cut(s) 272, 280, 360, 390, 440
BspQI GCTCTTC 1 cut(s) 165
BspT104I TTCGAA 1 cut(s) 13
BspTI CTTAAG 1 cut(s) 5
BssECI CCNNGG 1 cut(s) 277
BssMI GATC 5 cut(s) 270, 300, 350, 380, 456
Bst2UI CCWGG 1 cut(s) 488
Bst4CI ACNGT 1 cut(s) 95
Bst6I CTCTTC 7 cut(s) 165, 212, 290, 346, 370, 426, 450
BstAFI CTTAAG 1 cut(s) 5
BstBI TTCGAA 1 cut(s) 13
BstDEI CTNAG 7 cut(s) 259, 267, 321, 347, 377, 427, 507
BstDSI CCRYGG 1 cut(s) 277
BstF5I GGATG 4 cut(s) 335, 408, 415, 451
BstKTI GATC 5 cut(s) 273, 303, 353, 383, 459
BstMAI GTCTC 3 cut(s) 270, 380, 472
BstMBI GATC 5 cut(s) 270, 300, 350, 380, 456
BstNI CCWGG 1 cut(s) 488
BstSCI CCNGG 1 cut(s) 486
BstV1I GCAGC 1 cut(s) 159
BstV2I GAAGAC 2 cut(s) 176, 215
BsuRI GGCC 1 cut(s) 282
BtgI CCRYGG 1 cut(s) 277
BtsCI GGATG 4 cut(s) 335, 408, 415, 451
BtsIMutI CAGTG 1 cut(s) 100
CseI GACGC 1 cut(s) 380
CsiI ACCWGGT 1 cut(s) 486
Csp6I GTAC 2 cut(s) 53, 222
CviAII CATG 1 cut(s) 329
CviJI RGCY 9 cut(s) 10, 150, 175, 252, 282, 318, 325, 398, 405
CviKI_1 RGCY 9 cut(s) 10, 150, 175, 252, 282, 318, 325, 398, 405
CviQI GTAC 2 cut(s) 53, 222
DdeI CTNAG 7 cut(s) 259, 267, 321, 347, 377, 427, 507
DpnI GATC 5 cut(s) 272, 302, 352, 382, 458
DpnII GATC 5 cut(s) 270, 300, 350, 380, 456
DriI GACNNNNNGTC 1 cut(s) 262
Eam1104I CTCTTC 7 cut(s) 165, 212, 290, 346, 370, 426, 450
Eam1105I GACNNNNNGTC 1 cut(s) 262
EarI CTCTTC 7 cut(s) 165, 212, 290, 346, 370, 426, 450
EciI GGCGGA 1 cut(s) 219
EcoRII CCWGG 1 cut(s) 486
Esp3I CGTCTC 1 cut(s) 472
FaeI CATG 1 cut(s) 332
FaiI YATR 3 cut(s) 190, 195, 330
FatI CATG 1 cut(s) 328
FauI CCCGC 1 cut(s) 127
Fnu4HI GCNGC 3 cut(s) 76, 148, 162
FokI GGATG 4 cut(s) 322, 395, 402, 438
Fsp4HI GCNGC 3 cut(s) 76, 148, 162
FspBI CTAG 1 cut(s) 21
GluI GCNGC 3 cut(s) 76, 148, 162
GsuI CTGGAG 1 cut(s) 298
HaeIII GGCC 1 cut(s) 282
HgaI GACGC 1 cut(s) 380
Hin1II CATG 1 cut(s) 332
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 1 cut(s) 8
HinfI GANTC 2 cut(s) 257, 263
Hpy166II GTNNAC 1 cut(s) 91
Hpy188I TCNGA 8 cut(s) 50, 262, 270, 350, 380, 430, 456, 472
Hpy8I GTNNAC 1 cut(s) 91
HpyCH4III ACNGT 1 cut(s) 95
HpyF3I CTNAG 7 cut(s) 259, 267, 321, 347, 377, 427, 507
Hsp92II CATG 1 cut(s) 332
Kzo9I GATC 5 cut(s) 270, 300, 350, 380, 456
LguI GCTCTTC 1 cut(s) 165
LmnI GCTCC 3 cut(s) 330, 403, 410
LpnPI CCDG 4 cut(s) 328, 408, 473, 500
Lsp1109I GCAGC 1 cut(s) 159
LweI GCATC 1 cut(s) 460
MabI ACCWGGT 1 cut(s) 486
MaeI CTAG 1 cut(s) 21
MalI GATC 5 cut(s) 272, 302, 352, 382, 458
MboI GATC 5 cut(s) 270, 300, 350, 380, 456
MluCI AATT 1 cut(s) 460
MlyI GAGTC 2 cut(s) 251, 272
MmeI TCCRAC 2 cut(s) 28, 97
MseI TTAA 3 cut(s) 6, 26, 514
MspCI CTTAAG 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 488
MvaI CCWGG 1 cut(s) 488
NdeII GATC 5 cut(s) 270, 300, 350, 380, 456
NlaIII CATG 1 cut(s) 332
NspV TTCGAA 1 cut(s) 13
PciSI GCTCTTC 1 cut(s) 165
PkrI GCNGC 3 cut(s) 77, 149, 163
PleI GAGTC 2 cut(s) 251, 271
PpsI GAGTC 2 cut(s) 251, 271
Psp6I CCWGG 1 cut(s) 486
PspGI CCWGG 1 cut(s) 486
RsaI GTAC 2 cut(s) 54, 223
RsaNI GTAC 2 cut(s) 53, 222
SapI GCTCTTC 1 cut(s) 165
SaqAI TTAA 3 cut(s) 6, 26, 514
SatI GCNGC 3 cut(s) 76, 148, 162
Sau3AI GATC 5 cut(s) 270, 300, 350, 380, 456
ScaI AGTACT 1 cut(s) 54
SchI GAGTC 2 cut(s) 251, 272
ScrFI CCNGG 1 cut(s) 488
SexAI ACCWGGT 1 cut(s) 486
SfaNI GCATC 1 cut(s) 460
SfuI TTCGAA 1 cut(s) 13
SmlI CTYRAG 1 cut(s) 5
SmoI CTYRAG 1 cut(s) 5
Sse9I AATT 1 cut(s) 460
SsiI CCGC 6 cut(s) 75, 107, 134, 161, 230, 449
SspMI CTAG 1 cut(s) 21
StyD4I CCNGG 1 cut(s) 486
TaaI ACNGT 1 cut(s) 95
TaqI TCGA 6 cut(s) 13, 239, 273, 303, 353, 383
TasI AATT 1 cut(s) 460
TatI WGTACW 2 cut(s) 52, 221
TauI GCSGC 2 cut(s) 78, 164
Tru1I TTAA 3 cut(s) 6, 26, 514
Tru9I TTAA 3 cut(s) 6, 26, 514
TscAI CASTG 1 cut(s) 100
TseI GCWGC 1 cut(s) 147
TspDTI ATGAA 2 cut(s) 183, 230
TspGWI ACGGA 1 cut(s) 99
TspRI CASTG 1 cut(s) 100
Vha464I CTTAAG 1 cut(s) 5
XspI CTAG 1 cut(s) 21
ZrmI AGTACT 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.