RLG00000020191

Transmembrane protein 87A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016015
Physical Location & Seq
Forward (+)
62255174 .. 62257800
2627 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020191

Sequence Viewer

Length: 1518 bp
ATGTGGTACTCTGCTCTTCTTCTTGTTCTTCTTCTTGTGCTCTGCCTTGTGAGTGTGGAAGCCTCCGTCCACCACTACGCCGGAGACAGATTCGTCTCCAAAGGCAACGCCTTTGTCGTCCACGGCGGCAGCGAGGGAATTTACTCTTCTGACCCTGCCGACTACTTCGCCAAGGACAACTCTTACATACGCTTTGAGAAGGTTACGTTTCGGAGAAGCAAAGAATCGGCTAATTTCAGCTCTGGGCCTGTCCATGCCATTGTTTTTGAGGTGGAAGATAGAGAGAACATTGGGGGTTCAGCATATGGAGGTCAAAGAGCAGTATGTTGCACTGGGGATCTTGCAAAGCTCGGTGTGTGTAAGCAAGGGGATATCATTCACCGCCCGTCCACCAAGAACCCAGGTTGGCCTCAAGTTTTTGGTGTTTCATTTGAAGTAAATGAGGAAGTTGCCGCATTGCCATCCAAGTTTATACCGATTCATAAGACTGGGATGTATAATTTGTATTTCATACATTGTGATTTGAAGTTGAAGGATTTGGTGGTTGAGGGGAATACTATATGGAAAAACCCTACCGGGTATTTGCCTGGTAGAATGGCACCGTTTATACTTTTTTACGGGTTTATGTCCCTTGCTTTTGTCATACTTGGGATCTTTTGGTTCTCACAGTATGCAAGGTTTTGGAGAGAAGTTCTTCCATTGCAGAATTGCATCACACTAGTCATAACACTAGGTATGCTTGAGATGGCTTTATGGTATTTTGAGTATGCTGAATTCAATGAGACTGGGGTCAGGCCAACTGGGATCACTGTATGGGCAGTTTCATTTGGTGCAGTTAAACGAACAGTAGCGCGTTTAATACTTCTCATGGTCTCCATGGGCTATGGGGTTGTAAGGCCATCCCTAGGTGGGCTTACATCAAAAGTGGTGTTTCTTGGATTAACATTCTTCTTGGCAACTGAGGTTCTTGAGATGGTTGAAAACGTTGGTGCAGTTAGTGATCTTTCTGGAAAGGCAAGGTTGTTCTTGGTTCTTCCTGTGGCAATATTGGATGCTTTCTTCATTCTTTGGATTTTTACTTCTCTCTCTGCAACTTTAAATAAGCTTCAGGCTAGGCGAATGATGGTTAAAGTAGATATATACAGGAAGTTCACCAACGCTTTGGCAGTAACTGTTGTTGTATCTGTGGGCTGGATATGCTATGAGCTATATCACAAATCAAATGATGTTTACAATGAGCAGTGGCAGAATGCATGGATCATCCCTGCCTTCTGGCAAGTTTTATCTTTCTCTCTCCTATGTGTCATCTGTGCGCTTTGGGCACCATCCCAGAACTCAACAAGATATGCTTACTCTGGAGATGCAAGTGAAGAGTTTGACAGGGATGAAACTACTTTGACACTCATAAAACCATCTCTACTGCCTTCTAAGGATGTCCATAGAGCAACAGAATCTAGAGCAGTGCAAAGCAGCAATGGATTAACAAATGGTGATGAGGAAGAGGACAAGATAGAGTAA

Protein Analysis

506

Amino Acids

56.54

Weight (kDa)

6.19

Isoelectric Point (pI)

38.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GOST_TM PF06814 201 - 446 7.8e-90 GOST, seven transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016015)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72480
fragaria_vesca FvH4_6g35090
malus_domestica MD09G1178800.v1.1 MD17G1164600.v1.1
prunus_persica Prupe.3G038100_v2.0.a1
pyrus_communis pycom09g09720 pycom17g15770
rosa_chinensis RchiOBHm_Chr2g0146271
rosa_laevigata RLG00000020191
rosa_multiflora Rmu_sc0002054.1_g000015
rosa_roxburghii Rroxscaffold_2G00100190
rosa_rugosa Rorug02G0394100
rosa_samantha Rh2BG462500 Rh2DG471700
rosa_wichuraiana Rw2G036750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
AccB1I GGYRCC 2 cut(s) 598, 1321
AccII CGCG 1 cut(s) 853
AciI CCGC 3 cut(s) 126, 382, 453
AclI AACGTT 1 cut(s) 984
AclWI GGATC 4 cut(s) 345, 659, 812, 1265
AcsI RAATTY 2 cut(s) 138, 773
AcuI CTGAAG 1 cut(s) 1091
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 2 cut(s) 905, 909
AgsI TTSAA 5 cut(s) 434, 526, 532, 778, 980
AhlI ACTAGT 1 cut(s) 718
AjnI CCWGG 2 cut(s) 400, 586
AluBI AGCT 4 cut(s) 240, 349, 1105, 1207
AluI AGCT 4 cut(s) 240, 349, 1105, 1207
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 4 cut(s) 78, 100, 776, 877
AlwI GGATC 4 cut(s) 345, 659, 812, 1265
AlwNI CAGNNNCTG 1 cut(s) 1172
AoxI GGCC 4 cut(s) 245, 407, 794, 896
ApeKI GCWGC 2 cut(s) 129, 1470
ApoI RAATTY 2 cut(s) 138, 773
Asp700I GAANNNNTTC 1 cut(s) 693
AspA2I CCTAGG 1 cut(s) 904
AspLEI GCGC 2 cut(s) 853, 1315
AspS9I GGNCC 1 cut(s) 245
AsuC2I CCSGG 1 cut(s) 577
AsuHPI GGTGA 3 cut(s) 371, 1144, 1502
AvrII CCTAGG 1 cut(s) 904
BaeGI GKGCMC 1 cut(s) 1324
BanI GGYRCC 2 cut(s) 598, 1321
Bbv12I GWGCWC 1 cut(s) 42
BbvI GCAGC 2 cut(s) 141, 1482
BccI CCATC 7 cut(s) 469, 739, 907, 967, 1117, 1333, 1420
BceAI ACGGC 1 cut(s) 139
BciT130I CCWGG 2 cut(s) 402, 588
BcnI CCSGG 1 cut(s) 577
BcoDI GTCTC 4 cut(s) 78, 100, 776, 877
BcuI ACTAGT 1 cut(s) 718
BfaI CTAG 5 cut(s) 719, 731, 905, 1113, 1455
BisI GCNGC 4 cut(s) 127, 130, 453, 1471
BlnI CCTAGG 1 cut(s) 904
BlsI GCNGC 4 cut(s) 128, 131, 454, 1472
Bme1390I CCNGG 3 cut(s) 402, 577, 588
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 2 cut(s) 600, 1323
BmrFI CCNGG 3 cut(s) 402, 577, 588
BmrI ACTGGG 4 cut(s) 342, 498, 795, 810
BmsI GCATC 3 cut(s) 720, 1042, 1351
BmuI ACTGGG 4 cut(s) 342, 498, 795, 810
BoxI GACNNNNGTC 1 cut(s) 788
BpmI CTGGAG 1 cut(s) 1377
BpuEI CTTGAG 3 cut(s) 396, 761, 989
BpuMI CCSGG 1 cut(s) 577
BsaI GGTCTC 1 cut(s) 877
BsaJI CCNNGG 5 cut(s) 121, 171, 400, 876, 904
Bsc4I CCNNNNNNNGG 2 cut(s) 905, 909
Bse1I ACTGG 4 cut(s) 337, 493, 790, 805
Bse3DI GCAATG 3 cut(s) 455, 698, 1480
BseBI CCWGG 2 cut(s) 402, 588
BseDI CCNNGG 5 cut(s) 121, 171, 400, 876, 904
BseGI GGATG 8 cut(s) 461, 498, 899, 1057, 1260, 1325, 1390, 1438
BseLI CCNNNNNNNGG 2 cut(s) 905, 909
BseMI GCAATG 3 cut(s) 455, 698, 1480
BseMII CTCAG 1 cut(s) 951
BseNI ACTGG 4 cut(s) 337, 493, 790, 805
BseSI GKGCMC 1 cut(s) 1324
BseXI GCAGC 2 cut(s) 141, 1482
BsgI GTGCAG 2 cut(s) 852, 1011
Bsh1236I CGCG 1 cut(s) 853
BshFI GGCC 4 cut(s) 247, 409, 796, 898
BshNI GGYRCC 2 cut(s) 598, 1321
BsiHKAI GWGCWC 1 cut(s) 42
BsiSI CCGG 2 cut(s) 81, 576
BslFI GGGAC 1 cut(s) 613
BslI CCNNNNNNNGG 2 cut(s) 905, 909
BsmAI GTCTC 4 cut(s) 78, 100, 776, 877
BsmBI CGTCTC 1 cut(s) 100
BsmFI GGGAC 1 cut(s) 613
BsmI GAATGC 1 cut(s) 1255
BsnI GGCC 4 cut(s) 247, 409, 796, 898
Bso31I GGTCTC 1 cut(s) 877
Bsp1286I GDGCHC 2 cut(s) 42, 1324
Bsp143I GATC 5 cut(s) 337, 651, 804, 1000, 1257
Bsp19I CCATGG 1 cut(s) 876
BspACI CCGC 3 cut(s) 126, 382, 453
BspANI GGCC 4 cut(s) 247, 409, 796, 898
BspCNI CTCAG 1 cut(s) 952
BspFNI CGCG 1 cut(s) 853
BspLI GGNNCC 2 cut(s) 600, 1323
BspPI GGATC 4 cut(s) 345, 659, 812, 1265
BspQI GCTCTTC 1 cut(s) 21
BspT107I GGYRCC 2 cut(s) 598, 1321
BspTNI GGTCTC 1 cut(s) 877
BsrDI GCAATG 3 cut(s) 455, 698, 1480
BsrI ACTGG 4 cut(s) 337, 493, 790, 805
BssECI CCNNGG 5 cut(s) 121, 171, 400, 876, 904
BssMI GATC 5 cut(s) 337, 651, 804, 1000, 1257
BssT1I CCWWGG 3 cut(s) 171, 876, 904
Bst2UI CCWGG 2 cut(s) 402, 588
Bst4CI ACNGT 5 cut(s) 603, 669, 811, 847, 1174
Bst6I CTCTTC 4 cut(s) 21, 151, 1365, 1494
BstDEI CTNAG 2 cut(s) 960, 1428
BstDSI CCRYGG 2 cut(s) 121, 876
BstF5I GGATG 8 cut(s) 461, 498, 899, 1057, 1260, 1325, 1390, 1438
BstFNI CGCG 1 cut(s) 853
BstHHI GCGC 2 cut(s) 853, 1315
BstKTI GATC 5 cut(s) 340, 654, 807, 1003, 1260
BstMAI GTCTC 4 cut(s) 78, 100, 776, 877
BstMBI GATC 5 cut(s) 337, 651, 804, 1000, 1257
BstMWI GCNNNNNNNGC 2 cut(s) 1197, 1319
BstNI CCWGG 2 cut(s) 402, 588
BstPAI GACNNNNGTC 1 cut(s) 788
BstSCI CCNGG 3 cut(s) 400, 575, 586
BstSLI GKGCMC 1 cut(s) 1324
BstUI CGCG 1 cut(s) 853
BstV1I GCAGC 2 cut(s) 141, 1482
BstX2I RGATCY 2 cut(s) 337, 651
BstXI CCANNNNNNTGG 1 cut(s) 1162
BstYI RGATCY 2 cut(s) 337, 651
BsuRI GGCC 4 cut(s) 247, 409, 796, 898
BtgI CCRYGG 2 cut(s) 121, 876
BtsCI GGATG 8 cut(s) 461, 498, 899, 1057, 1260, 1325, 1390, 1438
BtsI GCAGTG 2 cut(s) 1247, 1467
BtsIMutI CAGTG 4 cut(s) 330, 807, 1247, 1467
CaiI CAGNNNCTG 1 cut(s) 1172
CfoI GCGC 2 cut(s) 853, 1315
Cfr13I GGNCC 1 cut(s) 245
Csp6I GTAC 1 cut(s) 7
CviAII CATG 4 cut(s) 254, 868, 877, 1254
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 2 cut(s) 960, 1428
DpnI GATC 5 cut(s) 339, 653, 806, 1002, 1259
DpnII GATC 5 cut(s) 337, 651, 804, 1000, 1257
DraI TTTAAA 1 cut(s) 1098
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
Eam1104I CTCTTC 4 cut(s) 21, 151, 1365, 1494
EarI CTCTTC 4 cut(s) 21, 151, 1365, 1494
Eco130I CCWWGG 3 cut(s) 171, 876, 904
Eco31I GGTCTC 1 cut(s) 877
Eco32I GATATC 1 cut(s) 373
Eco57I CTGAAG 1 cut(s) 1091
EcoRI GAATTC 1 cut(s) 773
EcoRII CCWGG 2 cut(s) 400, 586
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 3 cut(s) 171, 876, 904
EcoT22I ATGCAT 1 cut(s) 1255
ErhI CCWWGG 3 cut(s) 171, 876, 904
Esp3I CGTCTC 1 cut(s) 100
FaeI CATG 4 cut(s) 257, 871, 880, 1257
FalI AAGNNNNNCTT 2 cut(s) 1333, 1365
FaqI GGGAC 1 cut(s) 613
FatI CATG 4 cut(s) 253, 867, 876, 1253
FauNDI CATATG 1 cut(s) 304
Fnu4HI GCNGC 4 cut(s) 127, 130, 453, 1471
FokI GGATG 8 cut(s) 448, 505, 886, 1064, 1247, 1312, 1397, 1445
Fsp4HI GCNGC 4 cut(s) 127, 130, 453, 1471
FspBI CTAG 5 cut(s) 719, 731, 905, 1113, 1455
GlaI GCGC 2 cut(s) 852, 1314
GluI GCNGC 4 cut(s) 127, 130, 453, 1471
GsuI CTGGAG 1 cut(s) 1377
HaeIII GGCC 4 cut(s) 247, 409, 796, 898
HapII CCGG 2 cut(s) 81, 576
HhaI GCGC 2 cut(s) 853, 1315
Hin1II CATG 4 cut(s) 257, 871, 880, 1257
Hin6I GCGC 2 cut(s) 851, 1313
HinP1I GCGC 2 cut(s) 851, 1313
HindIII AAGCTT 1 cut(s) 1103
HinfI GANTC 4 cut(s) 90, 224, 478, 1451
HpaII CCGG 2 cut(s) 81, 576
HphI GGTGA 3 cut(s) 371, 1144, 1502
Hpy166II GTNNAC 5 cut(s) 70, 121, 390, 1152, 1231
Hpy188I TCNGA 2 cut(s) 151, 213
Hpy188III TCNNGA 4 cut(s) 968, 1008, 1356, 1455
Hpy8I GTNNAC 5 cut(s) 70, 121, 390, 1152, 1231
HpyAV CCTTC 4 cut(s) 193, 526, 1279, 1434
HpyCH4III ACNGT 5 cut(s) 603, 669, 811, 847, 1174
HpyCH4IV ACGT 2 cut(s) 206, 984
HpyF10VI GCNNNNNNNGC 2 cut(s) 1197, 1319
HpyF3I CTNAG 2 cut(s) 960, 1428
HpySE526I ACGT 2 cut(s) 206, 984
Hsp92II CATG 4 cut(s) 257, 871, 880, 1257
HspAI GCGC 2 cut(s) 851, 1313
Kzo9I GATC 5 cut(s) 337, 651, 804, 1000, 1257
LguI GCTCTTC 1 cut(s) 21
Lsp1109I GCAGC 2 cut(s) 141, 1482
LweI GCATC 3 cut(s) 720, 1042, 1351
MaeI CTAG 5 cut(s) 719, 731, 905, 1113, 1455
MaeII ACGT 2 cut(s) 206, 984
MaeIII GTNAC 2 cut(s) 202, 1168
MalI GATC 5 cut(s) 339, 653, 806, 1002, 1259
MboI GATC 5 cut(s) 337, 651, 804, 1000, 1257
MflI RGATCY 2 cut(s) 337, 651
MhlI GDGCHC 2 cut(s) 42, 1324
MluCI AATT 5 cut(s) 138, 232, 499, 706, 773
Mph1103I ATGCAT 1 cut(s) 1255
MroXI GAANNNNTTC 1 cut(s) 693
MseI TTAA 6 cut(s) 837, 857, 941, 1097, 1128, 1481
MspI CCGG 2 cut(s) 81, 576
MspR9I CCNGG 3 cut(s) 402, 577, 588
Mva1269I GAATGC 1 cut(s) 1255
MvaI CCWGG 2 cut(s) 402, 588
MvnI CGCG 1 cut(s) 853
MwoI GCNNNNNNNGC 2 cut(s) 1197, 1319
NciI CCSGG 1 cut(s) 577
NcoI CCATGG 1 cut(s) 876
NdeI CATATG 1 cut(s) 304
NdeII GATC 5 cut(s) 337, 651, 804, 1000, 1257
NlaIII CATG 4 cut(s) 257, 871, 880, 1257
NlaIV GGNNCC 2 cut(s) 600, 1323
NsiI ATGCAT 1 cut(s) 1255
PciSI GCTCTTC 1 cut(s) 21
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 129
PctI GAATGC 1 cut(s) 1255
PdmI GAANNNNTTC 1 cut(s) 693
PfeI GAWTC 4 cut(s) 90, 224, 478, 1451
PkrI GCNGC 4 cut(s) 128, 131, 454, 1472
PshAI GACNNNNGTC 1 cut(s) 788
Psp1406I AACGTT 1 cut(s) 984
Psp6I CCWGG 2 cut(s) 400, 586
PspGI CCWGG 2 cut(s) 400, 586
PspN4I GGNNCC 2 cut(s) 600, 1323
PspPI GGNCC 1 cut(s) 245
PstNI CAGNNNCTG 1 cut(s) 1172
PsuI RGATCY 2 cut(s) 337, 651
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
SapI GCTCTTC 1 cut(s) 21
SaqAI TTAA 6 cut(s) 837, 857, 941, 1097, 1128, 1481
SatI GCNGC 4 cut(s) 127, 130, 453, 1471
Sau3AI GATC 5 cut(s) 337, 651, 804, 1000, 1257
Sau96I GGNCC 1 cut(s) 245
ScrFI CCNGG 3 cut(s) 402, 577, 588
SduI GDGCHC 2 cut(s) 42, 1324
SfaNI GCATC 3 cut(s) 720, 1042, 1351
SmlI CTYRAG 3 cut(s) 411, 740, 968
SmoI CTYRAG 3 cut(s) 411, 740, 968
SpeI ACTAGT 1 cut(s) 718
Sse9I AATT 5 cut(s) 138, 232, 499, 706, 773
SsiI CCGC 3 cut(s) 126, 382, 453
SspI AATATT 1 cut(s) 1047
SspMI CTAG 5 cut(s) 719, 731, 905, 1113, 1455
StyD4I CCNGG 3 cut(s) 400, 575, 586
StyI CCWWGG 3 cut(s) 171, 876, 904
TaaI ACNGT 5 cut(s) 603, 669, 811, 847, 1174
TaiI ACGT 2 cut(s) 209, 987
TasI AATT 5 cut(s) 138, 232, 499, 706, 773
TauI GCSGC 2 cut(s) 129, 455
TfiI GAWTC 4 cut(s) 90, 224, 478, 1451
Tru1I TTAA 6 cut(s) 837, 857, 941, 1097, 1128, 1481
Tru9I TTAA 6 cut(s) 837, 857, 941, 1097, 1128, 1481
TscAI CASTG 4 cut(s) 337, 814, 1247, 1467
TseI GCWGC 2 cut(s) 129, 1470
TspDTI ATGAA 6 cut(s) 417, 470, 499, 813, 1051, 1401
TspGWI ACGGA 1 cut(s) 55
TspRI CASTG 4 cut(s) 337, 814, 1247, 1467
XapI RAATTY 2 cut(s) 138, 773
XbaI TCTAGA 1 cut(s) 1454
XmaJI CCTAGG 1 cut(s) 904
XmnI GAANNNNTTC 1 cut(s) 693
XspI CTAG 5 cut(s) 719, 731, 905, 1113, 1455
Zsp2I ATGCAT 1 cut(s) 1255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.