Rh2BG462500

Transmembrane protein 87A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0016015
Physical Location & Seq
Forward (+)
65405341 .. 65407941
2601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG462500.1

Sequence Viewer

Length: 1509 bp
ATGTGGTACTCTGCTCTTCTTCTTCTTGTGCTCTGCCTTCTGAGTGTGGAAGCCTCCGTCCACCACTACGCCGGAGACAGATTCGTCGCCAAAGGCAACGCCTTTGTCGTCCACGGCGGCAGCGAGGGAATTTACTCTTCTGCCCCTGCCGACGACTCCGCCAAGGACAACTCTTACATACGCTTTGAGAAGGTTACGTTTCGGAGAAGCAAAGAATCGGCTAATTTCAGCTCTGGGCCTGTCCATACCATTGTTTTTGAGGTGGAAGATAGAGAGAACATTGGGGGTTCAGCATATGGAGGTCAAAGAGCAGTATGTTGCACTGGGGATCTTGCGAAGCTCGGTGTGTGTAAGCAAGGCGATATCATTCACCGCCCGTCCACCAAGAACCCAGGTTGGCCTCAAGTTTTTGGTGTTTCGTTTGAAGTAGATGAGGAAGTTGTCGCATTGCCATCCAAGTTCATACCGATTCATAAGACTGGGATGTATAATTTGTATTTCATACATTGTGATTTGAAGTTGAAGGATTTGGTGGTTGAGGGGAATACTATATGGAAAAACCCTACCGGGTATTTGCCTGGTAGAATGGCACCGTTTATGCTTTTTTACGGGTTTATGTCTCTTGCTTTTTTCATACTTGGGATCTTTTGGTTCTCACAGTATGCAAGGTTTTGGAGAGAAGTTCTTCCGTTGCAGAATTGCATCACACTAGTCATAACACTAGGTATGCTTGAGATGGCTTTATGGTATTTTGAGTATGCTGAATTCAATGAGACTGGGGTCAGGCCAACTGGGATCACTGTATGGGCAGTTTCATTTGGTGCAGTTAAACGAACAGTAGCGCGTTTAATACTTCTCATGGTCTCCATGGGCTATGGGGTTGTAAGGCCATCCCTAGGTGGGCTTACATCAAAAGTGGTGTTTCTTGGATTAACATTCTTCTTGGCAACTGAGGTTCTTGAGATGGTTGAAAACGTTGGTGCAGTTAGTGATCTTTCTGGAAAGGCAAGGTTGTTCTTGGTTCTTCCTGTGGCAATATTGGATGCTTTCTTCATTCTTTGGATTTTTACTTCTCTCTCTGCAACTTTAAATAAGCTTCAGGCTAGGCGAATGATGGTTAAAGTAGATATATACAGGAAGTTCACCAACGCTTTGGCAGTAACTGTTGTTGTATCTGTGGGCTGGATATGCTATGAGCTATATCACAAATCAAATGATGTTTACAATGAGCAGTGGCAGAATGCATGGATCATCCCTGCCTTCTGGCAAGTTTTATCCTTCTCTCTCCTATGTGTCATCTGTGCTCTTTGGGCACCATCCCAGAACTCAACAAGATATGCTTACTCTGGAGATGCAAGTGAAGAGTTTGACAGGGATGAAACTACTTTGACCCTCATAAAACCATCTCTACTGCCTTCTAAGGATGTCCATAGAGCAACAGAAGCTAGAGCGGTGCAAAGCAGCAATGGAGTAACAAATGGTGATGAGGAAGAGGACAAGATAGAGTAA

Protein Analysis

502

Amino Acids

56.16

Weight (kDa)

6.05

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GOST_TM PF06814 198 - 443 3.6e-90 GOST, seven transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016015)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72480
fragaria_vesca FvH4_6g35090
malus_domestica MD09G1178800.v1.1 MD17G1164600.v1.1
prunus_persica Prupe.3G038100_v2.0.a1
pyrus_communis pycom09g09720 pycom17g15770
rosa_chinensis RchiOBHm_Chr2g0146271
rosa_laevigata RLG00000020191
rosa_multiflora Rmu_sc0002054.1_g000015
rosa_roxburghii Rroxscaffold_2G00100190
rosa_rugosa Rorug02G0394100
rosa_samantha Rh2BG462500 Rh2DG471700
rosa_wichuraiana Rw2G036750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 83
AccB1I GGYRCC 2 cut(s) 589, 1312
AccBSI CCGCTC 1 cut(s) 1451
AccII CGCG 1 cut(s) 844
AciI CCGC 4 cut(s) 117, 159, 373, 1451
AclI AACGTT 1 cut(s) 975
AclWI GGATC 4 cut(s) 336, 650, 803, 1256
AcsI RAATTY 2 cut(s) 129, 764
AcuI CTGAAG 1 cut(s) 1082
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 2 cut(s) 896, 900
AgsI TTSAA 5 cut(s) 425, 517, 523, 769, 971
AhlI ACTAGT 1 cut(s) 709
AjnI CCWGG 2 cut(s) 391, 577
AluBI AGCT 5 cut(s) 231, 340, 1096, 1198, 1445
AluI AGCT 5 cut(s) 231, 340, 1096, 1198, 1445
Alw21I GWGCWC 2 cut(s) 33, 1306
Alw26I GTCTC 4 cut(s) 69, 624, 767, 868
AlwI GGATC 4 cut(s) 336, 650, 803, 1256
AlwNI CAGNNNCTG 1 cut(s) 1163
AoxI GGCC 4 cut(s) 236, 398, 785, 887
ApeKI GCWGC 2 cut(s) 120, 1461
ApoI RAATTY 2 cut(s) 129, 764
Asp700I GAANNNNTTC 1 cut(s) 684
AspA2I CCTAGG 1 cut(s) 895
AspLEI GCGC 1 cut(s) 844
AspS9I GGNCC 1 cut(s) 236
AsuC2I CCSGG 1 cut(s) 568
AsuHPI GGTGA 3 cut(s) 362, 1135, 1493
AvrII CCTAGG 1 cut(s) 895
BaeGI GKGCMC 1 cut(s) 1315
BanI GGYRCC 2 cut(s) 589, 1312
Bbv12I GWGCWC 2 cut(s) 33, 1306
BbvI GCAGC 2 cut(s) 132, 1473
BccI CCATC 7 cut(s) 460, 730, 898, 958, 1108, 1324, 1411
BceAI ACGGC 1 cut(s) 130
BciT130I CCWGG 2 cut(s) 393, 579
BcnI CCSGG 1 cut(s) 568
BcoDI GTCTC 4 cut(s) 69, 624, 767, 868
BcuI ACTAGT 1 cut(s) 709
BfaI CTAG 5 cut(s) 710, 722, 896, 1104, 1446
BisI GCNGC 3 cut(s) 118, 121, 1462
BlnI CCTAGG 1 cut(s) 895
BlsI GCNGC 3 cut(s) 119, 122, 1463
Bme1390I CCNGG 3 cut(s) 393, 568, 579
BmgT120I GGNCC 1 cut(s) 236
BmiI GGNNCC 2 cut(s) 591, 1314
BmrFI CCNGG 3 cut(s) 393, 568, 579
BmrI ACTGGG 4 cut(s) 333, 489, 786, 801
BmsI GCATC 3 cut(s) 711, 1033, 1342
BmuI ACTGGG 4 cut(s) 333, 489, 786, 801
BoxI GACNNNNGTC 1 cut(s) 779
BpmI CTGGAG 1 cut(s) 1368
BpuEI CTTGAG 3 cut(s) 387, 752, 980
BpuMI CCSGG 1 cut(s) 568
BsaI GGTCTC 1 cut(s) 868
BsaJI CCNNGG 5 cut(s) 112, 162, 391, 867, 895
Bsc4I CCNNNNNNNGG 2 cut(s) 896, 900
Bse1I ACTGG 4 cut(s) 328, 484, 781, 796
Bse3DI GCAATG 2 cut(s) 446, 1471
BseBI CCWGG 2 cut(s) 393, 579
BseDI CCNNGG 5 cut(s) 112, 162, 391, 867, 895
BseGI GGATG 8 cut(s) 452, 489, 890, 1048, 1251, 1316, 1381, 1429
BseLI CCNNNNNNNGG 2 cut(s) 896, 900
BseMI GCAATG 2 cut(s) 446, 1471
BseMII CTCAG 2 cut(s) 32, 942
BseNI ACTGG 4 cut(s) 328, 484, 781, 796
BseSI GKGCMC 1 cut(s) 1315
BseXI GCAGC 2 cut(s) 132, 1473
BsgI GTGCAG 2 cut(s) 843, 1002
Bsh1236I CGCG 1 cut(s) 844
BshFI GGCC 4 cut(s) 238, 400, 787, 889
BshNI GGYRCC 2 cut(s) 589, 1312
BsiHKAI GWGCWC 2 cut(s) 33, 1306
BsiSI CCGG 2 cut(s) 72, 567
BslI CCNNNNNNNGG 2 cut(s) 896, 900
BsmAI GTCTC 4 cut(s) 69, 624, 767, 868
BsmI GAATGC 1 cut(s) 1246
BsnI GGCC 4 cut(s) 238, 400, 787, 889
Bso31I GGTCTC 1 cut(s) 868
Bsp1286I GDGCHC 3 cut(s) 33, 1306, 1315
Bsp143I GATC 5 cut(s) 328, 642, 795, 991, 1248
Bsp19I CCATGG 1 cut(s) 867
BspACI CCGC 4 cut(s) 117, 159, 373, 1451
BspANI GGCC 4 cut(s) 238, 400, 787, 889
BspCNI CTCAG 2 cut(s) 33, 943
BspFNI CGCG 1 cut(s) 844
BspLI GGNNCC 2 cut(s) 591, 1314
BspPI GGATC 4 cut(s) 336, 650, 803, 1256
BspQI GCTCTTC 1 cut(s) 21
BspT107I GGYRCC 2 cut(s) 589, 1312
BspTNI GGTCTC 1 cut(s) 868
BsrBI CCGCTC 1 cut(s) 1451
BsrDI GCAATG 2 cut(s) 446, 1471
BsrI ACTGG 4 cut(s) 328, 484, 781, 796
BssECI CCNNGG 5 cut(s) 112, 162, 391, 867, 895
BssMI GATC 5 cut(s) 328, 642, 795, 991, 1248
BssT1I CCWWGG 3 cut(s) 162, 867, 895
Bst2UI CCWGG 2 cut(s) 393, 579
Bst4CI ACNGT 5 cut(s) 594, 660, 802, 838, 1165
Bst6I CTCTTC 4 cut(s) 21, 142, 1356, 1485
BstDEI CTNAG 3 cut(s) 41, 951, 1419
BstDSI CCRYGG 2 cut(s) 112, 867
BstF5I GGATG 8 cut(s) 452, 489, 890, 1048, 1251, 1316, 1381, 1429
BstFNI CGCG 1 cut(s) 844
BstHHI GCGC 1 cut(s) 844
BstKTI GATC 5 cut(s) 331, 645, 798, 994, 1251
BstMAI GTCTC 4 cut(s) 69, 624, 767, 868
BstMBI GATC 5 cut(s) 328, 642, 795, 991, 1248
BstMWI GCNNNNNNNGC 3 cut(s) 1188, 1310, 1442
BstNI CCWGG 2 cut(s) 393, 579
BstPAI GACNNNNGTC 1 cut(s) 779
BstSCI CCNGG 3 cut(s) 391, 566, 577
BstSLI GKGCMC 1 cut(s) 1315
BstUI CGCG 1 cut(s) 844
BstV1I GCAGC 2 cut(s) 132, 1473
BstX2I RGATCY 2 cut(s) 328, 642
BstXI CCANNNNNNTGG 1 cut(s) 1153
BstYI RGATCY 2 cut(s) 328, 642
BsuRI GGCC 4 cut(s) 238, 400, 787, 889
BtgI CCRYGG 2 cut(s) 112, 867
BtsCI GGATG 8 cut(s) 452, 489, 890, 1048, 1251, 1316, 1381, 1429
BtsI GCAGTG 1 cut(s) 1238
BtsIMutI CAGTG 3 cut(s) 321, 798, 1238
CaiI CAGNNNCTG 1 cut(s) 1163
CfoI GCGC 1 cut(s) 844
Cfr13I GGNCC 1 cut(s) 236
Csp6I GTAC 1 cut(s) 7
CviAII CATG 3 cut(s) 859, 868, 1245
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 3 cut(s) 41, 951, 1419
DpnI GATC 5 cut(s) 330, 644, 797, 993, 1250
DpnII GATC 5 cut(s) 328, 642, 795, 991, 1248
DraI TTTAAA 1 cut(s) 1089
DrdI GACNNNNNNGTC 1 cut(s) 83
DseDI GACNNNNNNGTC 1 cut(s) 83
Eam1104I CTCTTC 4 cut(s) 21, 142, 1356, 1485
EarI CTCTTC 4 cut(s) 21, 142, 1356, 1485
EciI GGCGGA 1 cut(s) 148
Eco130I CCWWGG 3 cut(s) 162, 867, 895
Eco31I GGTCTC 1 cut(s) 868
Eco32I GATATC 1 cut(s) 364
Eco57I CTGAAG 1 cut(s) 1082
EcoRI GAATTC 1 cut(s) 764
EcoRII CCWGG 2 cut(s) 391, 577
EcoRV GATATC 1 cut(s) 364
EcoT14I CCWWGG 3 cut(s) 162, 867, 895
EcoT22I ATGCAT 1 cut(s) 1246
ErhI CCWWGG 3 cut(s) 162, 867, 895
FaeI CATG 3 cut(s) 862, 871, 1248
FalI AAGNNNNNCTT 2 cut(s) 1324, 1356
FatI CATG 3 cut(s) 858, 867, 1244
FauNDI CATATG 1 cut(s) 295
Fnu4HI GCNGC 3 cut(s) 118, 121, 1462
FokI GGATG 8 cut(s) 439, 496, 877, 1055, 1238, 1303, 1388, 1436
Fsp4HI GCNGC 3 cut(s) 118, 121, 1462
FspBI CTAG 5 cut(s) 710, 722, 896, 1104, 1446
GlaI GCGC 1 cut(s) 843
GluI GCNGC 3 cut(s) 118, 121, 1462
GsuI CTGGAG 1 cut(s) 1368
HaeIII GGCC 4 cut(s) 238, 400, 787, 889
HapII CCGG 2 cut(s) 72, 567
HhaI GCGC 1 cut(s) 844
Hin1II CATG 3 cut(s) 862, 871, 1248
Hin6I GCGC 1 cut(s) 842
HinP1I GCGC 1 cut(s) 842
HindIII AAGCTT 1 cut(s) 1094
HinfI GANTC 4 cut(s) 81, 155, 215, 469
HpaII CCGG 2 cut(s) 72, 567
HphI GGTGA 3 cut(s) 362, 1135, 1493
Hpy166II GTNNAC 5 cut(s) 61, 112, 381, 1143, 1222
Hpy188I TCNGA 2 cut(s) 42, 204
Hpy188III TCNNGA 3 cut(s) 959, 999, 1347
Hpy8I GTNNAC 5 cut(s) 61, 112, 381, 1143, 1222
Hpy99I CGWCG 2 cut(s) 89, 155
HpyAV CCTTC 6 cut(s) 47, 184, 517, 1270, 1288, 1425
HpyCH4III ACNGT 5 cut(s) 594, 660, 802, 838, 1165
HpyCH4IV ACGT 2 cut(s) 197, 975
HpyF10VI GCNNNNNNNGC 3 cut(s) 1188, 1310, 1442
HpyF3I CTNAG 3 cut(s) 41, 951, 1419
HpySE526I ACGT 2 cut(s) 197, 975
Hsp92II CATG 3 cut(s) 862, 871, 1248
HspAI GCGC 1 cut(s) 842
Kzo9I GATC 5 cut(s) 328, 642, 795, 991, 1248
LguI GCTCTTC 1 cut(s) 21
Lsp1109I GCAGC 2 cut(s) 132, 1473
LweI GCATC 3 cut(s) 711, 1033, 1342
MaeI CTAG 5 cut(s) 710, 722, 896, 1104, 1446
MaeII ACGT 2 cut(s) 197, 975
MaeIII GTNAC 3 cut(s) 193, 1159, 1471
MalI GATC 5 cut(s) 330, 644, 797, 993, 1250
MbiI CCGCTC 1 cut(s) 1451
MboI GATC 5 cut(s) 328, 642, 795, 991, 1248
MflI RGATCY 2 cut(s) 328, 642
MhlI GDGCHC 3 cut(s) 33, 1306, 1315
MluCI AATT 5 cut(s) 129, 223, 490, 697, 764
MlyI GAGTC 1 cut(s) 149
Mph1103I ATGCAT 1 cut(s) 1246
MroXI GAANNNNTTC 1 cut(s) 684
MseI TTAA 5 cut(s) 828, 848, 932, 1088, 1119
MspI CCGG 2 cut(s) 72, 567
MspR9I CCNGG 3 cut(s) 393, 568, 579
Mva1269I GAATGC 1 cut(s) 1246
MvaI CCWGG 2 cut(s) 393, 579
MvnI CGCG 1 cut(s) 844
MwoI GCNNNNNNNGC 3 cut(s) 1188, 1310, 1442
NciI CCSGG 1 cut(s) 568
NcoI CCATGG 1 cut(s) 867
NdeI CATATG 1 cut(s) 295
NdeII GATC 5 cut(s) 328, 642, 795, 991, 1248
NlaIII CATG 3 cut(s) 862, 871, 1248
NlaIV GGNNCC 2 cut(s) 591, 1314
NsiI ATGCAT 1 cut(s) 1246
PciSI GCTCTTC 1 cut(s) 21
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 120
PctI GAATGC 1 cut(s) 1246
PdmI GAANNNNTTC 1 cut(s) 684
PfeI GAWTC 3 cut(s) 81, 215, 469
PkrI GCNGC 3 cut(s) 119, 122, 1463
PleI GAGTC 1 cut(s) 149
PpsI GAGTC 1 cut(s) 149
PshAI GACNNNNGTC 1 cut(s) 779
Psp1406I AACGTT 1 cut(s) 975
Psp6I CCWGG 2 cut(s) 391, 577
PspGI CCWGG 2 cut(s) 391, 577
PspN4I GGNNCC 2 cut(s) 591, 1314
PspPI GGNCC 1 cut(s) 236
PstNI CAGNNNCTG 1 cut(s) 1163
PsuI RGATCY 2 cut(s) 328, 642
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
SapI GCTCTTC 1 cut(s) 21
SaqAI TTAA 5 cut(s) 828, 848, 932, 1088, 1119
SatI GCNGC 3 cut(s) 118, 121, 1462
Sau3AI GATC 5 cut(s) 328, 642, 795, 991, 1248
Sau96I GGNCC 1 cut(s) 236
SchI GAGTC 1 cut(s) 149
ScrFI CCNGG 3 cut(s) 393, 568, 579
SduI GDGCHC 3 cut(s) 33, 1306, 1315
SfaNI GCATC 3 cut(s) 711, 1033, 1342
SmlI CTYRAG 3 cut(s) 402, 731, 959
SmoI CTYRAG 3 cut(s) 402, 731, 959
SpeI ACTAGT 1 cut(s) 709
Sse9I AATT 5 cut(s) 129, 223, 490, 697, 764
SsiI CCGC 4 cut(s) 117, 159, 373, 1451
SspI AATATT 1 cut(s) 1038
SspMI CTAG 5 cut(s) 710, 722, 896, 1104, 1446
StyD4I CCNGG 3 cut(s) 391, 566, 577
StyI CCWWGG 3 cut(s) 162, 867, 895
TaaI ACNGT 5 cut(s) 594, 660, 802, 838, 1165
TaiI ACGT 2 cut(s) 200, 978
TasI AATT 5 cut(s) 129, 223, 490, 697, 764
TauI GCSGC 1 cut(s) 120
TfiI GAWTC 3 cut(s) 81, 215, 469
Tru1I TTAA 5 cut(s) 828, 848, 932, 1088, 1119
Tru9I TTAA 5 cut(s) 828, 848, 932, 1088, 1119
TscAI CASTG 3 cut(s) 328, 805, 1238
TseI GCWGC 2 cut(s) 120, 1461
TspDTI ATGAA 7 cut(s) 451, 461, 490, 622, 804, 1042, 1392
TspGWI ACGGA 2 cut(s) 46, 678
TspRI CASTG 3 cut(s) 328, 805, 1238
XapI RAATTY 2 cut(s) 129, 764
XmaJI CCTAGG 1 cut(s) 895
XmnI GAANNNNTTC 1 cut(s) 684
XspI CTAG 5 cut(s) 710, 722, 896, 1104, 1446
Zsp2I ATGCAT 1 cut(s) 1246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.