RLG00000022017

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018917
Physical Location & Seq
Forward (+)
79990889 .. 79991904
1016 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022017

Sequence Viewer

Length: 498 bp
ATGGCTGCAGCTCCAAGTGATTCCATACCCTCTGTATATAGGGCTTGGGTCCATTCAGAGTATGGAAAAACTGCAGATGTTCTGAAATTTGATCCAAATGTGGCTGTTCCTGAAATTAAAGAGGACCAGGTGCTGATCAAGGTAGTTGCTGCTTCTCTTAACCCAATTGATTTCATGAGAGCTCTTAGTTTCTTCAAGTATGATATGGCTGGTGTGGTGGTGAAGGTAGGAAGCCAAGTGCAGAAGTTTAAGGTGGGGGATGAAGTGCTGGGGTCTTTGGCAGAGTACACTGCTGCAGGAGAAACAATGTTGGCTCTCAAACCCAAAAACCTGAGCTTTATTGAAGCTGCTAGCCTTCCCTTAGCCATTGAGACTGCGTATGGAGCGCTTGAAAGAGCGGAGCTTTCGGCTGGTAAATCTGTCCTTGTTTTGGGAGGCGCTGGGGTGGGGGGTGTTGGAACACGTGATTCAGGTACATTCCGATTTCATGATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

166

Amino Acids

17.41

Weight (kDa)

5.39

Isoelectric Point (pI)

17.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 41 - 101 6.5e-10 Alcohol dehydrogenase GroES-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018917)

Species Orthologous Gene IDs
prunus_persica Prupe.3G284400_v2.0.a1
rosa_laevigata RLG00000022017
rosa_multiflora Rmu_sc0006783.1_g000003
rosa_roxburghii Rroxscaffold_2G00080620
rosa_rugosa Rorug02G0554800
rosa_samantha Rh2AG628900 Rh2AG635700 Rh2BG649100 Rh2CG608000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 398
AciI CCGC 1 cut(s) 398
AclWI GGATC 1 cut(s) 86
AcsI RAATTY 1 cut(s) 86
AcvI CACGTG 1 cut(s) 464
AfaI GTAC 2 cut(s) 287, 475
AfeI AGCGCT 1 cut(s) 387
AfiI CCNNNNNNNGG 1 cut(s) 430
AflIII ACRYGT 1 cut(s) 461
AgsI TTSAA 3 cut(s) 196, 344, 392
AjnI CCWGG 1 cut(s) 126
AluBI AGCT 5 cut(s) 11, 182, 336, 347, 403
AluI AGCT 5 cut(s) 11, 182, 336, 347, 403
Alw21I GWGCWC 1 cut(s) 184
Alw26I GTCTC 1 cut(s) 365
AlwI GGATC 1 cut(s) 86
AlwNI CAGNNNCTG 1 cut(s) 133
Aor51HI AGCGCT 1 cut(s) 387
ApeKI GCWGC 5 cut(s) 5, 8, 149, 293, 347
ApoI RAATTY 1 cut(s) 86
AspLEI GCGC 2 cut(s) 388, 440
AspS9I GGNCC 2 cut(s) 49, 124
AsuHPI GGTGA 1 cut(s) 232
AsuNHI GCTAGC 1 cut(s) 350
AvaII GGWCC 2 cut(s) 49, 124
BanII GRGCYC 1 cut(s) 184
BbrPI CACGTG 1 cut(s) 464
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 4 cut(s) 20, 136, 280, 334
BciT130I CCWGG 1 cut(s) 128
BclI TGATCA 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 365
BfaI CTAG 1 cut(s) 351
BfmI CTRYAG 3 cut(s) 6, 72, 294
BfoI RGCGCY 2 cut(s) 389, 441
BisI GCNGC 5 cut(s) 6, 9, 150, 294, 348
BlsI GCNGC 5 cut(s) 7, 10, 151, 295, 349
Bme1390I CCNGG 1 cut(s) 128
Bme18I GGWCC 2 cut(s) 49, 124
BmgT120I GGNCC 2 cut(s) 49, 124
BmiI GGNNCC 1 cut(s) 50
BmrFI CCNGG 1 cut(s) 128
BmsI GCATC 1 cut(s) 481
BmtI GCTAGC 1 cut(s) 354
Bpu10I CCTNAGC 2 cut(s) 332, 361
BsaAI YACGTR 1 cut(s) 464
Bsc4I CCNNNNNNNGG 1 cut(s) 430
BseBI CCWGG 1 cut(s) 128
BseGI GGATG 1 cut(s) 265
BseLI CCNNNNNNNGG 1 cut(s) 430
BseMII CTCAG 1 cut(s) 323
BseXI GCAGC 4 cut(s) 20, 136, 280, 334
BseYI CCCAGC 2 cut(s) 268, 440
BsgI GTGCAG 1 cut(s) 260
BsiHKAI GWGCWC 1 cut(s) 184
BslI CCNNNNNNNGG 1 cut(s) 430
BsmAI GTCTC 1 cut(s) 365
Bsp1286I GDGCHC 1 cut(s) 184
Bsp143I GATC 2 cut(s) 91, 135
BspACI CCGC 1 cut(s) 398
BspCNI CTCAG 1 cut(s) 324
BspHI TCATGA 2 cut(s) 174, 487
BspLI GGNNCC 1 cut(s) 50
BspMAI CTGCAG 3 cut(s) 10, 76, 298
BspOI GCTAGC 1 cut(s) 354
BspPI GGATC 1 cut(s) 86
BsrBI CCGCTC 1 cut(s) 398
BssMI GATC 2 cut(s) 91, 135
Bst2UI CCWGG 1 cut(s) 128
BstBAI YACGTR 1 cut(s) 464
BstC8I GCNNGC 1 cut(s) 352
BstDEI CTNAG 3 cut(s) 185, 332, 361
BstF5I GGATG 1 cut(s) 265
BstH2I RGCGCY 2 cut(s) 389, 441
BstHHI GCGC 2 cut(s) 388, 440
BstKTI GATC 2 cut(s) 94, 138
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 2 cut(s) 91, 135
BstMWI GCNNNNNNNGC 1 cut(s) 383
BstNI CCWGG 1 cut(s) 128
BstSCI CCNGG 1 cut(s) 126
BstSFI CTRYAG 3 cut(s) 6, 72, 294
BstV1I GCAGC 4 cut(s) 20, 136, 280, 334
BtsCI GGATG 1 cut(s) 265
BtsI GCAGTG 1 cut(s) 288
BtsIMutI CAGTG 1 cut(s) 288
Cac8I GCNNGC 1 cut(s) 352
CaiI CAGNNNCTG 1 cut(s) 133
CciI TCATGA 2 cut(s) 174, 487
CfoI GCGC 2 cut(s) 388, 440
Cfr13I GGNCC 2 cut(s) 49, 124
CsiI ACCWGGT 1 cut(s) 126
Csp6I GTAC 2 cut(s) 286, 474
CviAII CATG 2 cut(s) 175, 488
CviQI GTAC 2 cut(s) 286, 474
DdeI CTNAG 3 cut(s) 185, 332, 361
DpnI GATC 2 cut(s) 93, 137
DpnII GATC 2 cut(s) 91, 135
Ecl136II GAGCTC 1 cut(s) 182
Eco24I GRGCYC 1 cut(s) 184
Eco47I GGWCC 2 cut(s) 49, 124
Eco47III AGCGCT 1 cut(s) 387
Eco53kI GAGCTC 1 cut(s) 182
Eco72I CACGTG 1 cut(s) 464
EcoICRI GAGCTC 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 126
EcoT38I GRGCYC 1 cut(s) 184
FaeI CATG 2 cut(s) 178, 491
FaiI YATR 9 cut(s) 26, 37, 39, 63, 176, 201, 206, 381, 489
FatI CATG 2 cut(s) 174, 487
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 5 cut(s) 6, 9, 150, 294, 348
FokI GGATG 1 cut(s) 272
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 5 cut(s) 6, 9, 150, 294, 348
FspBI CTAG 1 cut(s) 351
GlaI GCGC 2 cut(s) 387, 439
GluI GCNGC 5 cut(s) 6, 9, 150, 294, 348
GsaI CCCAGC 2 cut(s) 272, 444
HaeII RGCGCY 2 cut(s) 389, 441
HhaI GCGC 2 cut(s) 388, 440
Hin1II CATG 2 cut(s) 178, 491
Hin6I GCGC 2 cut(s) 386, 438
HinP1I GCGC 2 cut(s) 386, 438
HinfI GANTC 2 cut(s) 20, 467
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 1 cut(s) 288
Hpy188I TCNGA 3 cut(s) 58, 84, 482
Hpy188III TCNNGA 3 cut(s) 110, 175, 488
Hpy8I GTNNAC 1 cut(s) 288
HpyAV CCTTC 2 cut(s) 217, 365
HpyCH4IV ACGT 1 cut(s) 463
HpyCH4V TGCA 4 cut(s) 8, 74, 241, 296
HpyF10VI GCNNNNNNNGC 1 cut(s) 383
HpyF3I CTNAG 3 cut(s) 185, 332, 361
HpySE526I ACGT 1 cut(s) 463
Hsp92II CATG 2 cut(s) 178, 491
HspAI GCGC 2 cut(s) 386, 438
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 2 cut(s) 91, 135
LmnI GCTCC 3 cut(s) 16, 383, 400
Lsp1109I GCAGC 4 cut(s) 20, 136, 280, 334
LweI GCATC 1 cut(s) 481
MabI ACCWGGT 1 cut(s) 126
MaeI CTAG 1 cut(s) 351
MaeII ACGT 1 cut(s) 463
MalI GATC 2 cut(s) 93, 137
MbiI CCGCTC 1 cut(s) 398
MboI GATC 2 cut(s) 91, 135
MboII GAAGA 1 cut(s) 184
MfeI CAATTG 1 cut(s) 165
MhlI GDGCHC 1 cut(s) 184
MluCI AATT 3 cut(s) 86, 114, 165
MmeI TCCRAC 1 cut(s) 436
MnlI CCTC 3 cut(s) 40, 115, 428
MseI TTAA 3 cut(s) 117, 159, 249
MspR9I CCNGG 1 cut(s) 128
MunI CAATTG 1 cut(s) 165
MvaI CCWGG 1 cut(s) 128
MwoI GCNNNNNNNGC 1 cut(s) 383
NdeII GATC 2 cut(s) 91, 135
NheI GCTAGC 1 cut(s) 350
NlaIII CATG 2 cut(s) 178, 491
NlaIV GGNNCC 1 cut(s) 50
PagI TCATGA 2 cut(s) 174, 487
PfeI GAWTC 2 cut(s) 20, 467
PkrI GCNGC 5 cut(s) 7, 10, 151, 295, 349
PmaCI CACGTG 1 cut(s) 464
PmlI CACGTG 1 cut(s) 464
Ppu21I YACGTR 1 cut(s) 464
Psp124BI GAGCTC 1 cut(s) 184
Psp6I CCWGG 1 cut(s) 126
PspCI CACGTG 1 cut(s) 464
PspFI CCCAGC 2 cut(s) 268, 440
PspGI CCWGG 1 cut(s) 126
PspN4I GGNNCC 1 cut(s) 50
PspPI GGNCC 2 cut(s) 49, 124
PstI CTGCAG 3 cut(s) 10, 76, 298
PstNI CAGNNNCTG 1 cut(s) 133
RsaI GTAC 2 cut(s) 287, 475
RsaNI GTAC 2 cut(s) 286, 474
SacI GAGCTC 1 cut(s) 184
SaqAI TTAA 3 cut(s) 117, 159, 249
SatI GCNGC 5 cut(s) 6, 9, 150, 294, 348
Sau3AI GATC 2 cut(s) 91, 135
Sau96I GGNCC 2 cut(s) 49, 124
ScrFI CCNGG 1 cut(s) 128
SduI GDGCHC 1 cut(s) 184
SexAI ACCWGGT 1 cut(s) 126
SfaNI GCATC 1 cut(s) 481
SfcI CTRYAG 3 cut(s) 6, 72, 294
SinI GGWCC 2 cut(s) 49, 124
Sse9I AATT 3 cut(s) 86, 114, 165
SsiI CCGC 1 cut(s) 398
SspMI CTAG 1 cut(s) 351
SstI GAGCTC 1 cut(s) 184
StyD4I CCNGG 1 cut(s) 126
TaiI ACGT 1 cut(s) 466
TasI AATT 3 cut(s) 86, 114, 165
TatI WGTACW 1 cut(s) 285
TfiI GAWTC 2 cut(s) 20, 467
Tru1I TTAA 3 cut(s) 117, 159, 249
Tru9I TTAA 3 cut(s) 117, 159, 249
TscAI CASTG 1 cut(s) 295
TseI GCWGC 5 cut(s) 5, 8, 149, 293, 347
TspDTI ATGAA 3 cut(s) 163, 276, 476
TspRI CASTG 1 cut(s) 295
VpaK11BI GGWCC 2 cut(s) 49, 124
XapI RAATTY 1 cut(s) 86
XcmI CCANNNNNNNNNTGG 1 cut(s) 59
XspI CTAG 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.