Rh2AG628900

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0018917
Physical Location & Seq
Forward (+)
85397904 .. 85398815
912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG628900.1

Sequence Viewer

Length: 471 bp
ATGGCTGCAGCTCCAAGTGATTCCATACCCTATGTATATGGGGCTTGGGTCTATTCAGAGTATGGAAAAACTGCAGATGTTCTGAAATTTGATCCAAATGTGGCTGTTCCTGAAATTAAAGAGGACCAGGTGCTGATCAAGGTAGTTGCTGCTTCTCTTAACCCAATTGATTTCATGAGAGCTCTTAGTTTCTTCAAGGACATAGAGGACAATTCCAGGTATGATGTGGCTGGTGTGGTGGTGAAGGTAGGAAGCCAAGTGCAGAAGTTTAAGGTGGGGGATGAAGTGTATGCTTGTTTGAGTAATAGCATTGAGATTCCCCAATATCATGCTAAGAATAGCCATGGGGATCTCAATGAGAAAGCATTGGTGAAACCAAGCAGGCTGGGGTCTTTGGCAGAGTACACTGCTGCAGGAGAAACCATGTTGGCTCTCAAACCCAAAAATCTGAGCTTGATTGAAGCATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

156

Amino Acids

17.02

Weight (kDa)

5.18

Isoelectric Point (pI)

31.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 41 - 147 4.3e-13 Alcohol dehydrogenase GroES-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018917)

Species Orthologous Gene IDs
prunus_persica Prupe.3G284400_v2.0.a1
rosa_laevigata RLG00000022017
rosa_multiflora Rmu_sc0006783.1_g000003
rosa_roxburghii Rroxscaffold_2G00080620
rosa_rugosa Rorug02G0554800
rosa_samantha Rh2AG628900 Rh2AG635700 Rh2BG649100 Rh2CG608000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 86, 357
AcsI RAATTY 1 cut(s) 86
AfaI GTAC 1 cut(s) 404
AgsI TTSAA 2 cut(s) 196, 461
AjnI CCWGG 2 cut(s) 126, 215
AluBI AGCT 3 cut(s) 11, 182, 453
AluI AGCT 3 cut(s) 11, 182, 453
Alw21I GWGCWC 1 cut(s) 184
AlwI GGATC 2 cut(s) 86, 357
AlwNI CAGNNNCTG 1 cut(s) 133
ApeKI GCWGC 4 cut(s) 5, 8, 149, 410
ApoI RAATTY 1 cut(s) 86
AspS9I GGNCC 1 cut(s) 124
AsuHPI GGTGA 2 cut(s) 253, 382
AvaII GGWCC 1 cut(s) 124
BanII GRGCYC 1 cut(s) 184
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 3 cut(s) 20, 136, 397
BciT130I CCWGG 2 cut(s) 128, 217
BclI TGATCA 1 cut(s) 135
BfaI CTAG 1 cut(s) 469
BfmI CTRYAG 3 cut(s) 6, 72, 411
BisI GCNGC 4 cut(s) 6, 9, 150, 411
BlsI GCNGC 4 cut(s) 7, 10, 151, 412
Bme1390I CCNGG 2 cut(s) 128, 217
Bme18I GGWCC 1 cut(s) 124
BmgT120I GGNCC 1 cut(s) 124
BmrFI CCNGG 2 cut(s) 128, 217
BsaJI CCNNGG 1 cut(s) 343
BseBI CCWGG 2 cut(s) 128, 217
BseDI CCNNGG 1 cut(s) 343
BseGI GGATG 1 cut(s) 286
BseMII CTCAG 1 cut(s) 440
BseXI GCAGC 3 cut(s) 20, 136, 397
BseYI CCCAGC 1 cut(s) 385
BsgI GTGCAG 1 cut(s) 281
BsiHKAI GWGCWC 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 184
Bsp143I GATC 3 cut(s) 91, 135, 349
Bsp19I CCATGG 1 cut(s) 343
BspCNI CTCAG 1 cut(s) 441
BspHI TCATGA 1 cut(s) 174
BspMAI CTGCAG 3 cut(s) 10, 76, 415
BspPI GGATC 2 cut(s) 86, 357
BssECI CCNNGG 1 cut(s) 343
BssMI GATC 3 cut(s) 91, 135, 349
BssT1I CCWWGG 1 cut(s) 343
Bst2UI CCWGG 2 cut(s) 128, 217
BstC8I GCNNGC 2 cut(s) 383, 466
BstDEI CTNAG 3 cut(s) 185, 333, 449
BstDSI CCRYGG 1 cut(s) 343
BstF5I GGATG 1 cut(s) 286
BstKTI GATC 3 cut(s) 94, 138, 352
BstMBI GATC 3 cut(s) 91, 135, 349
BstNI CCWGG 2 cut(s) 128, 217
BstNSI RCATGY 1 cut(s) 468
BstSCI CCNGG 2 cut(s) 126, 215
BstSFI CTRYAG 3 cut(s) 6, 72, 411
BstV1I GCAGC 3 cut(s) 20, 136, 397
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
BtgI CCRYGG 1 cut(s) 343
BtsCI GGATG 1 cut(s) 286
BtsI GCAGTG 1 cut(s) 405
BtsIMutI CAGTG 1 cut(s) 405
Cac8I GCNNGC 2 cut(s) 383, 466
CaiI CAGNNNCTG 1 cut(s) 133
CciI TCATGA 1 cut(s) 174
Cfr13I GGNCC 1 cut(s) 124
CsiI ACCWGGT 1 cut(s) 126
Csp6I GTAC 1 cut(s) 403
CviAII CATG 5 cut(s) 175, 329, 344, 424, 465
CviQI GTAC 1 cut(s) 403
DdeI CTNAG 3 cut(s) 185, 333, 449
DpnI GATC 3 cut(s) 93, 137, 351
DpnII GATC 3 cut(s) 91, 135, 349
Ecl136II GAGCTC 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 343
Eco24I GRGCYC 1 cut(s) 184
Eco47I GGWCC 1 cut(s) 124
Eco53kI GAGCTC 1 cut(s) 182
EcoICRI GAGCTC 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 126, 215
EcoT14I CCWWGG 1 cut(s) 343
EcoT38I GRGCYC 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 343
FaeI CATG 5 cut(s) 178, 332, 347, 427, 468
FatI CATG 5 cut(s) 174, 328, 343, 423, 464
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 4 cut(s) 6, 9, 150, 411
FokI GGATG 1 cut(s) 293
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 4 cut(s) 6, 9, 150, 411
FspBI CTAG 1 cut(s) 469
GluI GCNGC 4 cut(s) 6, 9, 150, 411
GsaI CCCAGC 1 cut(s) 389
Hin1II CATG 5 cut(s) 178, 332, 347, 427, 468
HinfI GANTC 2 cut(s) 20, 316
HphI GGTGA 2 cut(s) 253, 382
Hpy166II GTNNAC 1 cut(s) 405
Hpy188I TCNGA 3 cut(s) 58, 84, 450
Hpy188III TCNNGA 2 cut(s) 110, 175
Hpy8I GTNNAC 1 cut(s) 405
HpyAV CCTTC 1 cut(s) 238
HpyCH4V TGCA 4 cut(s) 8, 74, 262, 413
HpyF3I CTNAG 3 cut(s) 185, 333, 449
Hsp92II CATG 5 cut(s) 178, 332, 347, 427, 468
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 3 cut(s) 91, 135, 349
LmnI GCTCC 1 cut(s) 16
LpnPI CCDG 9 cut(s) 113, 123, 140, 202, 216, 229, 367, 371, 399
Lsp1109I GCAGC 3 cut(s) 20, 136, 397
MabI ACCWGGT 1 cut(s) 126
MaeI CTAG 1 cut(s) 469
MalI GATC 3 cut(s) 93, 137, 351
MboI GATC 3 cut(s) 91, 135, 349
MboII GAAGA 1 cut(s) 184
MfeI CAATTG 1 cut(s) 165
MflI RGATCY 1 cut(s) 349
MhlI GDGCHC 1 cut(s) 184
MluCI AATT 4 cut(s) 86, 114, 165, 211
MnlI CCTC 2 cut(s) 115, 199
MseI TTAA 3 cut(s) 117, 159, 270
MspR9I CCNGG 2 cut(s) 128, 217
MunI CAATTG 1 cut(s) 165
MvaI CCWGG 2 cut(s) 128, 217
NcoI CCATGG 1 cut(s) 343
NdeII GATC 3 cut(s) 91, 135, 349
NlaIII CATG 5 cut(s) 178, 332, 347, 427, 468
NspI RCATGY 1 cut(s) 468
PaeI GCATGC 1 cut(s) 468
PagI TCATGA 1 cut(s) 174
PfeI GAWTC 2 cut(s) 20, 316
PkrI GCNGC 4 cut(s) 7, 10, 151, 412
Psp124BI GAGCTC 1 cut(s) 184
Psp6I CCWGG 2 cut(s) 126, 215
PspFI CCCAGC 1 cut(s) 385
PspGI CCWGG 2 cut(s) 126, 215
PspPI GGNCC 1 cut(s) 124
PstI CTGCAG 3 cut(s) 10, 76, 415
PstNI CAGNNNCTG 1 cut(s) 133
PsuI RGATCY 1 cut(s) 349
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
SacI GAGCTC 1 cut(s) 184
SaqAI TTAA 3 cut(s) 117, 159, 270
SatI GCNGC 4 cut(s) 6, 9, 150, 411
Sau3AI GATC 3 cut(s) 91, 135, 349
Sau96I GGNCC 1 cut(s) 124
ScrFI CCNGG 2 cut(s) 128, 217
SduI GDGCHC 1 cut(s) 184
SetI ASST 8 cut(s) 13, 132, 144, 184, 221, 249, 276, 455
SexAI ACCWGGT 1 cut(s) 126
SfcI CTRYAG 3 cut(s) 6, 72, 411
SinI GGWCC 1 cut(s) 124
SphI GCATGC 1 cut(s) 468
Sse9I AATT 4 cut(s) 86, 114, 165, 211
SspMI CTAG 1 cut(s) 469
SstI GAGCTC 1 cut(s) 184
StyD4I CCNGG 2 cut(s) 126, 215
StyI CCWWGG 1 cut(s) 343
TasI AATT 4 cut(s) 86, 114, 165, 211
TatI WGTACW 1 cut(s) 402
TfiI GAWTC 2 cut(s) 20, 316
Tru1I TTAA 3 cut(s) 117, 159, 270
Tru9I TTAA 3 cut(s) 117, 159, 270
TscAI CASTG 1 cut(s) 412
TseI GCWGC 4 cut(s) 5, 8, 149, 410
TspDTI ATGAA 2 cut(s) 163, 297
TspRI CASTG 1 cut(s) 412
VpaK11BI GGWCC 1 cut(s) 124
XapI RAATTY 1 cut(s) 86
XceI RCATGY 1 cut(s) 468
XcmI CCANNNNNNNNNTGG 1 cut(s) 223
XspI CTAG 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.