Rorug02G0554800

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001188
Physical Location & Seq
Forward (+)
68189863 .. 68199372
9510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0554800.1

Sequence Viewer

Length: 1086 bp
ATGTACTCTTCCACCAATAATGACATTAGTAGTAGTGGTACTGTATTTCCTTTCCCACCTTATAACTTTCCCTTTTCCTCCTCTTCTTCTTCTCATAACAGCCACCACCCTCTTCTTCCTCAAGAAAATACAGCCTGTGGAGGCATTTTTGGCCTACACCGTGATCAATTTTCCCTATTAAACAATTGTTTGCTGTCACCCGGACCGGAAAGTACTATGGTCAATCTCGGAGGAGCTTCATCAAACGCGATCGTATCATCATCTGGACACTACCAGCAGCATTATGGTTATGGTAATAATATTAATCATCCCTATTTTGTTTCTAAGGAGGTGAGAGTACCGGTGGTTGCTCTGAAGAAAGACCGGCACAGCAAGATCTTCACTTCTCAGGGCTTGAGGGATCGGAGGGTAAGACTCTCCATCAATGTTGCCCGTGAGTTCTTCGATCTCCAAGACTTGCTAGGGTTTGACAAAGCCAGCAAAACCTTAGAGTGGCTGCTCAGCAATTCCAGAAAGGCCATCAAGGACCTAGAAAAGAGCAAGTGCAATAACAGTACTAGTATATCATCTTCTAGTACTTCGGACTGTGATGATGTTTTGGACATAAACAACGAGGCGTCCGATGACAAGAGGAGGAAGATGAAGGAGGCTGCTGCTATAATGGCCAGAGCAACGCGGGCAAAGGCTAGAGCAAGAGCAAGAGAAAGAACAAGAGAGAAGATGTGCAGTACTAGTACGAGGAGCCATCAGATCTGTAGCCAGTTGAGCTTGTTCAATGAGCTTGAACCTGCAGATATGAATAAAGAACCGGGTAGTACACTCCTATCTAATTGTCAAGCTGAATATCATGAATCTGTTGCGCTCAAAAGAAAGCTGAAGGCGCCGTCAGTTTCCAATTATCAAGATCGAAGCCTTGAATTGAACCCCAAAAGCACGGAACCTAGTTCAAATTGCTGCAGCCACATTAATATCCAATTTCCGAATGCAATATCTCATAATTATTGGGACATCAGCGGCAGTGTCTTCACATGCCCTAGCATTTGTGCCATTACCACCAACAAAAATCTTAATTCTACAGGGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

361

Amino Acids

39.96

Weight (kDa)

8.89

Isoelectric Point (pI)

52.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TCP PF03634 119 - 243 2.4e-36 TCP family transcription factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 63
Acc36I ACCTGC 1 cut(s) 796
AccB1I GGYRCC 1 cut(s) 880
AccII CGCG 2 cut(s) 248, 676
AciI CCGC 2 cut(s) 676, 1014
AclWI GGATC 1 cut(s) 408
AcoI YGGCCR 1 cut(s) 663
AcuI CTGAAG 2 cut(s) 374, 896
AcyI GRCGYC 2 cut(s) 617, 881
AfaI GTAC 9 cut(s) 5, 40, 214, 339, 556, 577, 730, 736, 817
AfiI CCNNNNNNNGG 1 cut(s) 492
AgeI ACCGGT 1 cut(s) 340
AgsI TTSAA 5 cut(s) 775, 785, 917, 922, 948
AhlI ACTAGT 2 cut(s) 557, 731
AluBI AGCT 5 cut(s) 236, 768, 781, 839, 874
AluI AGCT 5 cut(s) 236, 768, 781, 839, 874
AlwI GGATC 1 cut(s) 408
AoxI GGCC 3 cut(s) 151, 516, 663
ApeKI GCWGC 6 cut(s) 277, 496, 650, 653, 954, 957
AseI ATTAAT 2 cut(s) 303, 966
AsiGI ACCGGT 1 cut(s) 340
AspLEI GCGC 2 cut(s) 862, 883
AspS9I GGNCC 2 cut(s) 203, 526
AsuC2I CCSGG 2 cut(s) 201, 810
AsuHPI GGTGA 2 cut(s) 189, 343
AvaII GGWCC 2 cut(s) 203, 526
BalI TGGCCA 1 cut(s) 665
BanI GGYRCC 1 cut(s) 880
BarI GAAGNNNNNNTAC 2 cut(s) 553, 585
BbsI GAAGAC 1 cut(s) 1015
BbvI GCAGC 6 cut(s) 289, 483, 637, 640, 941, 969
BccI CCATC 3 cut(s) 428, 527, 753
BceAI ACGGC 1 cut(s) 868
BclI TGATCA 1 cut(s) 163
BcnI CCSGG 2 cut(s) 201, 810
BcuI ACTAGT 2 cut(s) 557, 731
BfaI CTAG 8 cut(s) 461, 530, 558, 573, 687, 732, 942, 1035
BfmI CTRYAG 4 cut(s) 754, 789, 955, 1074
BfoI RGCGCY 1 cut(s) 884
BfuAI ACCTGC 1 cut(s) 796
BglII AGATCT 2 cut(s) 375, 750
BisI GCNGC 7 cut(s) 278, 497, 651, 654, 955, 958, 1015
BlpI GCTNAGC 1 cut(s) 500
BlsI GCNGC 7 cut(s) 279, 498, 652, 655, 956, 959, 1016
BmcAI AGTACT 4 cut(s) 214, 556, 577, 730
Bme1390I CCNGG 2 cut(s) 201, 810
Bme18I GGWCC 2 cut(s) 203, 526
BmgT120I GGNCC 2 cut(s) 203, 526
BmiI GGNNCC 3 cut(s) 743, 882, 939
BmrFI CCNGG 2 cut(s) 201, 810
BpiI GAAGAC 1 cut(s) 1015
Bpu1102I GCTNAGC 1 cut(s) 500
BpuEI CTTGAG 2 cut(s) 105, 415
BpuMI CCSGG 2 cut(s) 201, 810
BsaHI GRCGYC 2 cut(s) 617, 881
BsaWI WCCGGW 2 cut(s) 205, 340
Bsc4I CCNNNNNNNGG 1 cut(s) 492
Bse118I RCCGGY 2 cut(s) 340, 363
Bse1I ACTGG 1 cut(s) 760
BseGI GGATG 1 cut(s) 307
BseLI CCNNNNNNNGG 1 cut(s) 492
BseMII CTCAG 2 cut(s) 401, 514
BseNI ACTGG 1 cut(s) 760
BseRI GAGGAG 4 cut(s) 70, 246, 646, 754
BseXI GCAGC 6 cut(s) 289, 483, 637, 640, 941, 969
BsgI GTGCAG 1 cut(s) 745
Bsh1236I CGCG 2 cut(s) 248, 676
Bsh1285I CGRYCG 1 cut(s) 252
BshFI GGCC 3 cut(s) 153, 518, 665
BshNI GGYRCC 1 cut(s) 880
BshTI ACCGGT 1 cut(s) 340
BsiEI CGRYCG 1 cut(s) 252
BsiSI CCGG 5 cut(s) 201, 206, 341, 364, 809
BslFI GGGAC 1 cut(s) 1019
BslI CCNNNNNNNGG 1 cut(s) 492
BsmFI GGGAC 1 cut(s) 1019
BsmI GAATGC 1 cut(s) 988
BsnI GGCC 3 cut(s) 153, 518, 665
Bsp143I GATC 7 cut(s) 163, 249, 375, 400, 445, 750, 904
Bsp1720I GCTNAGC 1 cut(s) 500
BspACI CCGC 2 cut(s) 676, 1014
BspANI GGCC 3 cut(s) 153, 518, 665
BspCNI CTCAG 2 cut(s) 400, 513
BspFNI CGCG 2 cut(s) 248, 676
BspHI TCATGA 1 cut(s) 847
BspLI GGNNCC 3 cut(s) 743, 882, 939
BspMAI CTGCAG 2 cut(s) 793, 959
BspMI ACCTGC 1 cut(s) 796
BspPI GGATC 1 cut(s) 408
BspT107I GGYRCC 1 cut(s) 880
BsrFI RCCGGY 2 cut(s) 340, 363
BsrI ACTGG 1 cut(s) 760
BssAI RCCGGY 2 cut(s) 340, 363
BssMI GATC 7 cut(s) 163, 249, 375, 400, 445, 750, 904
BssNI GRCGYC 2 cut(s) 617, 881
Bst4CI ACNGT 4 cut(s) 43, 161, 554, 587
Bst6I CTCTTC 3 cut(s) 13, 88, 117
BstACI GRCGYC 2 cut(s) 617, 881
BstC8I GCNNGC 2 cut(s) 478, 678
BstDEI CTNAG 4 cut(s) 324, 387, 487, 500
BstF5I GGATG 1 cut(s) 307
BstFNI CGCG 2 cut(s) 248, 676
BstH2I RGCGCY 1 cut(s) 884
BstHHI GCGC 2 cut(s) 862, 883
BstKTI GATC 7 cut(s) 166, 252, 378, 403, 448, 753, 907
BstMBI GATC 7 cut(s) 163, 249, 375, 400, 445, 750, 904
BstMCI CGRYCG 1 cut(s) 252
BstMWI GCNNNNNNNGC 5 cut(s) 150, 662, 677, 765, 880
BstNSI RCATGY 1 cut(s) 1032
BstSCI CCNGG 2 cut(s) 199, 808
BstSFI CTRYAG 4 cut(s) 754, 789, 955, 1074
BstUI CGCG 2 cut(s) 248, 676
BstV1I GCAGC 6 cut(s) 289, 483, 637, 640, 941, 969
BstV2I GAAGAC 1 cut(s) 1015
BstX2I RGATCY 2 cut(s) 375, 750
BstYI RGATCY 2 cut(s) 375, 750
BsuRI GGCC 3 cut(s) 153, 518, 665
BtsCI GGATG 1 cut(s) 307
BtsI GCAGTG 1 cut(s) 1024
BtsIMutI CAGTG 1 cut(s) 1024
BveI ACCTGC 1 cut(s) 796
Cac8I GCNNGC 2 cut(s) 478, 678
CciI TCATGA 1 cut(s) 847
CfoI GCGC 2 cut(s) 862, 883
Cfr10I RCCGGY 2 cut(s) 340, 363
Cfr13I GGNCC 2 cut(s) 203, 526
CpoI CGGWCCG 1 cut(s) 203
CseI GACGC 1 cut(s) 606
Csp6I GTAC 9 cut(s) 4, 39, 213, 338, 555, 576, 729, 735, 816
CspAI ACCGGT 1 cut(s) 340
CspI CGGWCCG 1 cut(s) 203
CviAII CATG 2 cut(s) 848, 1029
CviQI GTAC 9 cut(s) 4, 39, 213, 338, 555, 576, 729, 735, 816
DdeI CTNAG 4 cut(s) 324, 387, 487, 500
DinI GGCGCC 1 cut(s) 882
DpnI GATC 7 cut(s) 165, 251, 377, 402, 447, 752, 906
DpnII GATC 7 cut(s) 163, 249, 375, 400, 445, 750, 904
EaeI YGGCCR 1 cut(s) 663
Eam1104I CTCTTC 3 cut(s) 13, 88, 117
EarI CTCTTC 3 cut(s) 13, 88, 117
Eco47I GGWCC 2 cut(s) 203, 526
Eco57I CTGAAG 2 cut(s) 374, 896
EcoO109I RGGNCCY 1 cut(s) 526
EgeI GGCGCC 1 cut(s) 882
EheI GGCGCC 1 cut(s) 882
FaeI CATG 2 cut(s) 851, 1032
FaqI GGGAC 1 cut(s) 1019
FatI CATG 2 cut(s) 847, 1028
FauI CCCGC 1 cut(s) 669
FbaI TGATCA 1 cut(s) 163
Fnu4HI GCNGC 7 cut(s) 278, 497, 651, 654, 955, 958, 1015
FokI GGATG 1 cut(s) 294
Fsp4HI GCNGC 7 cut(s) 278, 497, 651, 654, 955, 958, 1015
FspBI CTAG 8 cut(s) 461, 530, 558, 573, 687, 732, 942, 1035
GlaI GCGC 2 cut(s) 861, 882
GluI GCNGC 7 cut(s) 278, 497, 651, 654, 955, 958, 1015
HaeII RGCGCY 1 cut(s) 884
HaeIII GGCC 3 cut(s) 153, 518, 665
HapII CCGG 5 cut(s) 201, 206, 341, 364, 809
HgaI GACGC 1 cut(s) 606
HhaI GCGC 2 cut(s) 862, 883
Hin1I GRCGYC 2 cut(s) 617, 881
Hin1II CATG 2 cut(s) 851, 1032
Hin6I GCGC 2 cut(s) 860, 881
HinP1I GCGC 2 cut(s) 860, 881
HinfI GANTC 2 cut(s) 414, 851
HpaII CCGG 5 cut(s) 201, 206, 341, 364, 809
HphI GGTGA 2 cut(s) 189, 343
Hpy166II GTNNAC 1 cut(s) 818
Hpy188I TCNGA 7 cut(s) 230, 354, 405, 583, 622, 750, 981
Hpy188III TCNNGA 5 cut(s) 122, 264, 510, 848, 902
Hpy8I GTNNAC 1 cut(s) 818
HpyAV CCTTC 2 cut(s) 637, 871
HpyCH4III ACNGT 4 cut(s) 43, 161, 554, 587
HpyCH4V TGCA 5 cut(s) 546, 726, 791, 957, 986
HpyF10VI GCNNNNNNNGC 5 cut(s) 150, 662, 677, 765, 880
HpyF3I CTNAG 4 cut(s) 324, 387, 487, 500
Hsp92I GRCGYC 2 cut(s) 617, 881
Hsp92II CATG 2 cut(s) 851, 1032
HspAI GCGC 2 cut(s) 860, 881
KasI GGCGCC 1 cut(s) 880
Ksp22I TGATCA 1 cut(s) 163
Kzo9I GATC 7 cut(s) 163, 249, 375, 400, 445, 750, 904
LmnI GCTCC 2 cut(s) 233, 741
Lsp1109I GCAGC 6 cut(s) 289, 483, 637, 640, 941, 969
MaeI CTAG 8 cut(s) 461, 530, 558, 573, 687, 732, 942, 1035
MaeIII GTNAC 1 cut(s) 195
MalI GATC 7 cut(s) 165, 251, 377, 402, 447, 752, 906
MboI GATC 7 cut(s) 163, 249, 375, 400, 445, 750, 904
MfeI CAATTG 1 cut(s) 184
MflI RGATCY 2 cut(s) 375, 750
MlsI TGGCCA 1 cut(s) 665
MluNI TGGCCA 1 cut(s) 665
Mly113I GGCGCC 1 cut(s) 881
MlyI GAGTC 1 cut(s) 408
Mox20I TGGCCA 1 cut(s) 665
MscI TGGCCA 1 cut(s) 665
MseI TTAA 5 cut(s) 179, 303, 966, 1068, 1084
Msp20I TGGCCA 1 cut(s) 665
MspA1I CMGCKG 1 cut(s) 1014
MspI CCGG 5 cut(s) 201, 206, 341, 364, 809
MspR9I CCNGG 2 cut(s) 201, 810
MunI CAATTG 1 cut(s) 184
Mva1269I GAATGC 1 cut(s) 988
MvnI CGCG 2 cut(s) 248, 676
MwoI GCNNNNNNNGC 5 cut(s) 150, 662, 677, 765, 880
NarI GGCGCC 1 cut(s) 881
NciI CCSGG 2 cut(s) 201, 810
NdeII GATC 7 cut(s) 163, 249, 375, 400, 445, 750, 904
NlaIII CATG 2 cut(s) 851, 1032
NlaIV GGNNCC 3 cut(s) 743, 882, 939
NmuCI GTSAC 1 cut(s) 195
NspI RCATGY 1 cut(s) 1032
PagI TCATGA 1 cut(s) 847
PcsI WCGNNNNNNNCGW 1 cut(s) 618
PctI GAATGC 1 cut(s) 988
PfeI GAWTC 1 cut(s) 851
PinAI ACCGGT 1 cut(s) 340
PkrI GCNGC 7 cut(s) 279, 498, 652, 655, 956, 959, 1016
Ple19I CGATCG 1 cut(s) 252
PleI GAGTC 1 cut(s) 408
PluTI GGCGCC 1 cut(s) 884
PpsI GAGTC 1 cut(s) 408
PpuMI RGGWCCY 1 cut(s) 526
PshBI ATTAAT 2 cut(s) 303, 966
PsiI TTATAA 1 cut(s) 63
Psp5II RGGWCCY 1 cut(s) 526
PspN4I GGNNCC 3 cut(s) 743, 882, 939
PspPI GGNCC 2 cut(s) 203, 526
PspPPI RGGWCCY 1 cut(s) 526
PstI CTGCAG 2 cut(s) 793, 959
PsuI RGATCY 2 cut(s) 375, 750
PvuI CGATCG 1 cut(s) 252
RsaI GTAC 9 cut(s) 5, 40, 214, 339, 556, 577, 730, 736, 817
RsaNI GTAC 9 cut(s) 4, 39, 213, 338, 555, 576, 729, 735, 816
Rsr2I CGGWCCG 1 cut(s) 203
RsrII CGGWCCG 1 cut(s) 203
SaqAI TTAA 5 cut(s) 179, 303, 966, 1068, 1084
SatI GCNGC 7 cut(s) 278, 497, 651, 654, 955, 958, 1015
Sau3AI GATC 7 cut(s) 163, 249, 375, 400, 445, 750, 904
Sau96I GGNCC 2 cut(s) 203, 526
ScaI AGTACT 4 cut(s) 214, 556, 577, 730
SchI GAGTC 1 cut(s) 408
ScrFI CCNGG 2 cut(s) 201, 810
SfcI CTRYAG 4 cut(s) 754, 789, 955, 1074
SfoI GGCGCC 1 cut(s) 882
SinI GGWCC 2 cut(s) 203, 526
SmlI CTYRAG 2 cut(s) 120, 394
SmoI CTYRAG 2 cut(s) 120, 394
SpeI ACTAGT 2 cut(s) 557, 731
SsiI CCGC 2 cut(s) 676, 1014
SspDI GGCGCC 1 cut(s) 880
SspI AATATT 1 cut(s) 301
SspMI CTAG 8 cut(s) 461, 530, 558, 573, 687, 732, 942, 1035
StyD4I CCNGG 2 cut(s) 199, 808
TaaI ACNGT 4 cut(s) 43, 161, 554, 587
TaqI TCGA 2 cut(s) 444, 907
TatI WGTACW 6 cut(s) 3, 212, 554, 575, 728, 815
TauI GCSGC 1 cut(s) 1017
TfiI GAWTC 1 cut(s) 851
Tru1I TTAA 5 cut(s) 179, 303, 966, 1068, 1084
Tru9I TTAA 5 cut(s) 179, 303, 966, 1068, 1084
TscAI CASTG 1 cut(s) 1024
TseFI GTSAC 1 cut(s) 195
TseI GCWGC 6 cut(s) 277, 496, 650, 653, 954, 957
Tsp45I GTSAC 1 cut(s) 195
TspDTI ATGAA 4 cut(s) 228, 656, 812, 864
TspGWI ACGGA 1 cut(s) 950
TspRI CASTG 1 cut(s) 1024
VpaK11BI GGWCC 2 cut(s) 203, 526
VspI ATTAAT 2 cut(s) 303, 966
XceI RCATGY 1 cut(s) 1032
XcmI CCANNNNNNNNNTGG 1 cut(s) 281
XspI CTAG 8 cut(s) 461, 530, 558, 573, 687, 732, 942, 1035
ZrmI AGTACT 4 cut(s) 214, 556, 577, 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.