Prupe.3G284900_v2.0.a1

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001188
Physical Location & Seq
Forward (+)
25804608 .. 25806567
1960 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G284900.1

Sequence Viewer

Length: 972 bp
ATGGCTGAGGCTTCGTGTGTGTCCACACCACCATCTGTAAACAAGGCTTGGATTTACAGAGAACATGGAGAAACTGCACATGTTTTGAAGTTTGAAACAAATGTGGCTGTTCCTCAAATAAAGGAAGACCAGGTGCTGATCAAGGTTGTTGCTGCAGCTCTTAACCCAATTGATGCCAAGAGGATCCTTGGCGTTTTGAGGGCCACTGACACTGCTCCGCCTATAGTTCCAGGATATGATGTGGCTGGTGTGGTGGTGAAGTTGGGAAGCAAAGCCAGCAAATTTAACATTGGGGATGAAGTGTATGGAGATATCAATGAGGAGAGTAGCCTAAATCTGAAGAAGTTTGGGACTTTGGCAGAGTACACTGCTGCAGAAGAAAGATTGTTGGCTCTGAAACCCAAAAACCTGAGCTTTGTGGAAGCTGCTAGCCTTCCGGCAGCTATTGAGACAGCCTATGAAGGGCTAAAACGAGTTGGGCTTTCTGCTGGCCAGTCCATTCTTGTTTTGGGAGGCGCTGGGGGTTGTGGAACTCATGTTATTCAGCTAGCAAAGCATGTTTTTGGTGCCTCCAGAGTAGCAGCCACTGCGAGCACTAAAAAACTTGATTTGCTGAGAAGCTTGGGTGCTGATTTGGCTATTGACTACACTAAGGAAAACGTTGAAGACCTGCCTGAGAAATTTGATGTGGTGTATGATGCAGTTGGTCAATCGGATAAGGCAGTGAAGGCAGTGAAAGAAGGGGGAAGGGTGGTGACATTAATAGGTCCAATAACACCACCAGCCTCCATGTTTGTGCTCACCTCAACTGGGTCCATTTTGGAGAAACTCAAGCCTTACTTGGAGAGTAGGAAGGTGAAGGCAGTGATTGATCCCACAGGCCCGTATCAATTTTCTAAGACCATAGACGCATTTGCCTACCTTCAAACTTCCAGGGCCACTGGAAAGGTGGTTGTGTATCCCATCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

324

Amino Acids

34.29

Weight (kDa)

8.3

Isoelectric Point (pI)

29.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 678
AccB1I GGYRCC 1 cut(s) 566
AciI CCGC 1 cut(s) 218
AclI AACGTT 1 cut(s) 660
AclWI GGATC 3 cut(s) 178, 191, 866
AcoI YGGCCR 1 cut(s) 490
AcsI RAATTY 2 cut(s) 281, 680
AcuI CTGAAG 1 cut(s) 359
AfaI GTAC 1 cut(s) 365
AfiI CCNNNNNNNGG 2 cut(s) 462, 810
AflIII ACRYGT 1 cut(s) 79
AgsI TTSAA 4 cut(s) 88, 95, 665, 926
AjnI CCWGG 3 cut(s) 129, 229, 932
AluBI AGCT 6 cut(s) 158, 414, 425, 443, 547, 621
AluI AGCT 6 cut(s) 158, 414, 425, 443, 547, 621
Alw21I GWGCWC 2 cut(s) 596, 801
Alw26I GTCTC 1 cut(s) 443
AlwI GGATC 3 cut(s) 178, 191, 866
AlwNI CAGNNNCTG 2 cut(s) 136, 587
AoxI GGCC 4 cut(s) 201, 490, 880, 936
ApeKI GCWGC 6 cut(s) 152, 155, 371, 425, 440, 581
ApoI RAATTY 2 cut(s) 281, 680
AseI ATTAAT 1 cut(s) 761
AspLEI GCGC 1 cut(s) 518
AspS9I GGNCC 5 cut(s) 201, 767, 813, 881, 936
AsuHPI GGTGA 4 cut(s) 268, 766, 793, 868
AsuNHI GCTAGC 2 cut(s) 428, 547
AvaII GGWCC 2 cut(s) 767, 813
BalI TGGCCA 1 cut(s) 492
BamHI GGATCC 1 cut(s) 183
BanI GGYRCC 1 cut(s) 566
BbsI GAAGAC 2 cut(s) 132, 672
Bbv12I GWGCWC 2 cut(s) 596, 801
BbvCI CCTCAGC 1 cut(s) 6
BbvI GCAGC 6 cut(s) 139, 167, 358, 412, 452, 593
BccI CCATC 2 cut(s) 40, 971
BciT130I CCWGG 3 cut(s) 131, 231, 934
BciVI GTATCC 1 cut(s) 969
BclI TGATCA 1 cut(s) 138
BcoDI GTCTC 1 cut(s) 443
BfaI CTAG 2 cut(s) 429, 548
BfmI CTRYAG 3 cut(s) 153, 222, 372
BfoI RGCGCY 1 cut(s) 519
BfuAI ACCTGC 1 cut(s) 678
BfuI GTATCC 1 cut(s) 969
BisI GCNGC 6 cut(s) 153, 156, 372, 426, 441, 582
BlsI GCNGC 6 cut(s) 154, 157, 373, 427, 442, 583
Bme1390I CCNGG 3 cut(s) 131, 231, 934
Bme18I GGWCC 2 cut(s) 767, 813
BmgT120I GGNCC 5 cut(s) 201, 767, 813, 881, 936
BmiI GGNNCC 3 cut(s) 185, 568, 814
BmrFI CCNGG 3 cut(s) 131, 231, 934
BmrI ACTGGG 1 cut(s) 819
BmsI GCATC 2 cut(s) 163, 688
BmtI GCTAGC 2 cut(s) 432, 551
BmuI ACTGGG 1 cut(s) 819
BpiI GAAGAC 2 cut(s) 132, 672
BpmI CTGGAG 1 cut(s) 556
Bpu10I CCTNAGC 2 cut(s) 6, 410
BpuEI CTTGAG 1 cut(s) 815
BsaJI CCNNGG 2 cut(s) 187, 933
Bsc4I CCNNNNNNNGG 2 cut(s) 462, 810
Bse1I ACTGG 3 cut(s) 493, 814, 946
BseBI CCWGG 3 cut(s) 131, 231, 934
BseDI CCNNGG 2 cut(s) 187, 933
BseGI GGATG 2 cut(s) 301, 963
BseLI CCNNNNNNNGG 2 cut(s) 462, 810
BseMII CTCAG 3 cut(s) 401, 605, 666
BseNI ACTGG 3 cut(s) 493, 814, 946
BseRI GAGGAG 1 cut(s) 335
BseXI GCAGC 6 cut(s) 139, 167, 358, 412, 452, 593
BseYI CCCAGC 1 cut(s) 518
BsgI GTGCAG 1 cut(s) 60
BshFI GGCC 4 cut(s) 203, 492, 882, 938
BshNI GGYRCC 1 cut(s) 566
BsiHKAI GWGCWC 2 cut(s) 596, 801
BsiSI CCGG 1 cut(s) 437
BslFI GGGAC 1 cut(s) 364
BslI CCNNNNNNNGG 2 cut(s) 462, 810
BsmAI GTCTC 1 cut(s) 443
BsmFI GGGAC 1 cut(s) 364
BsnI GGCC 4 cut(s) 203, 492, 882, 938
Bsp1286I GDGCHC 2 cut(s) 596, 801
Bsp143I GATC 3 cut(s) 138, 183, 871
BspACI CCGC 1 cut(s) 218
BspANI GGCC 4 cut(s) 203, 492, 882, 938
BspCNI CTCAG 3 cut(s) 402, 606, 667
BspLI GGNNCC 3 cut(s) 185, 568, 814
BspMAI CTGCAG 2 cut(s) 157, 376
BspMI ACCTGC 1 cut(s) 678
BspOI GCTAGC 2 cut(s) 432, 551
BspPI GGATC 3 cut(s) 178, 191, 866
BspT107I GGYRCC 1 cut(s) 566
BsrI ACTGG 3 cut(s) 493, 814, 946
BssECI CCNNGG 2 cut(s) 187, 933
BssMI GATC 3 cut(s) 138, 183, 871
BssT1I CCWWGG 1 cut(s) 187
Bst2UI CCWGG 3 cut(s) 131, 231, 934
BstAPI GCANNNNNTGC 1 cut(s) 587
BstC8I GCNNGC 5 cut(s) 277, 430, 490, 549, 592
BstDEI CTNAG 6 cut(s) 6, 410, 614, 651, 675, 897
BstF5I GGATG 2 cut(s) 301, 963
BstH2I RGCGCY 1 cut(s) 519
BstHHI GCGC 1 cut(s) 518
BstKTI GATC 3 cut(s) 141, 186, 874
BstMAI GTCTC 1 cut(s) 443
BstMBI GATC 3 cut(s) 138, 183, 871
BstMWI GCNNNNNNNGC 5 cut(s) 276, 553, 587, 635, 728
BstNI CCWGG 3 cut(s) 131, 231, 934
BstNSI RCATGY 2 cut(s) 83, 560
BstSCI CCNGG 3 cut(s) 129, 229, 932
BstSFI CTRYAG 3 cut(s) 153, 222, 372
BstV1I GCAGC 6 cut(s) 139, 167, 358, 412, 452, 593
BstV2I GAAGAC 2 cut(s) 132, 672
BstX2I RGATCY 1 cut(s) 183
BstYI RGATCY 1 cut(s) 183
BsuI GTATCC 1 cut(s) 969
BsuRI GGCC 4 cut(s) 203, 492, 882, 938
BtsCI GGATG 2 cut(s) 301, 963
BtsI GCAGTG 6 cut(s) 210, 366, 585, 729, 738, 870
BtsIMutI CAGTG 8 cut(s) 204, 210, 366, 585, 729, 738, 870, 939
BveI ACCTGC 1 cut(s) 678
Cac8I GCNNGC 5 cut(s) 277, 430, 490, 549, 592
CaiI CAGNNNCTG 2 cut(s) 136, 587
CfoI GCGC 1 cut(s) 518
Cfr13I GGNCC 5 cut(s) 201, 767, 813, 881, 936
CseI GACGC 1 cut(s) 917
CsiI ACCWGGT 1 cut(s) 129
Csp6I GTAC 1 cut(s) 364
CviAII CATG 5 cut(s) 65, 80, 536, 557, 790
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 6 cut(s) 6, 410, 614, 651, 675, 897
DpnI GATC 3 cut(s) 140, 185, 873
DpnII GATC 3 cut(s) 138, 183, 871
EaeI YGGCCR 1 cut(s) 490
EciI GGCGGA 1 cut(s) 207
Eco130I CCWWGG 1 cut(s) 187
Eco32I GATATC 1 cut(s) 313
Eco47I GGWCC 2 cut(s) 767, 813
Eco57I CTGAAG 1 cut(s) 359
EcoRII CCWGG 3 cut(s) 129, 229, 932
EcoRV GATATC 1 cut(s) 313
EcoT14I CCWWGG 1 cut(s) 187
ErhI CCWWGG 1 cut(s) 187
FaeI CATG 5 cut(s) 68, 83, 539, 560, 793
FalI AAGNNNNNCTT 2 cut(s) 824, 856
FaqI GGGAC 1 cut(s) 364
FatI CATG 5 cut(s) 64, 79, 535, 556, 789
FbaI TGATCA 1 cut(s) 138
Fnu4HI GCNGC 6 cut(s) 153, 156, 372, 426, 441, 582
FokI GGATG 2 cut(s) 308, 950
Fsp4HI GCNGC 6 cut(s) 153, 156, 372, 426, 441, 582
FspBI CTAG 2 cut(s) 429, 548
GlaI GCGC 1 cut(s) 517
GluI GCNGC 6 cut(s) 153, 156, 372, 426, 441, 582
GsaI CCCAGC 1 cut(s) 522
GsuI CTGGAG 1 cut(s) 556
HaeII RGCGCY 1 cut(s) 519
HaeIII GGCC 4 cut(s) 203, 492, 882, 938
HapII CCGG 1 cut(s) 437
HgaI GACGC 1 cut(s) 917
HhaI GCGC 1 cut(s) 518
Hin1II CATG 5 cut(s) 68, 83, 539, 560, 793
Hin6I GCGC 1 cut(s) 516
HinP1I GCGC 1 cut(s) 516
HindIII AAGCTT 1 cut(s) 619
HpaII CCGG 1 cut(s) 437
HphI GGTGA 4 cut(s) 268, 766, 793, 868
Hpy166II GTNNAC 3 cut(s) 24, 40, 366
Hpy188I TCNGA 3 cut(s) 339, 396, 715
Hpy188III TCNNGA 1 cut(s) 573
Hpy8I GTNNAC 3 cut(s) 24, 40, 366
HpyAV CCTTC 8 cut(s) 443, 455, 721, 734, 741, 847, 853, 932
HpyCH4IV ACGT 1 cut(s) 660
HpyCH4V TGCA 4 cut(s) 77, 155, 374, 701
HpyF10VI GCNNNNNNNGC 5 cut(s) 276, 553, 587, 635, 728
HpyF3I CTNAG 6 cut(s) 6, 410, 614, 651, 675, 897
HpySE526I ACGT 1 cut(s) 660
Hsp92II CATG 5 cut(s) 68, 83, 539, 560, 793
HspAI GCGC 1 cut(s) 516
Ksp22I TGATCA 1 cut(s) 138
Kzo9I GATC 3 cut(s) 138, 183, 871
LmnI GCTCC 1 cut(s) 220
Lsp1109I GCAGC 6 cut(s) 139, 167, 358, 412, 452, 593
LweI GCATC 2 cut(s) 163, 688
MabI ACCWGGT 1 cut(s) 129
MaeI CTAG 2 cut(s) 429, 548
MaeII ACGT 1 cut(s) 660
MaeIII GTNAC 1 cut(s) 754
MalI GATC 3 cut(s) 140, 185, 873
MboI GATC 3 cut(s) 138, 183, 871
MboII GAAGA 4 cut(s) 137, 352, 389, 677
MfeI CAATTG 1 cut(s) 168
MflI RGATCY 1 cut(s) 183
MhlI GDGCHC 2 cut(s) 596, 801
MlsI TGGCCA 1 cut(s) 492
MluCI AATT 4 cut(s) 168, 281, 680, 890
MluNI TGGCCA 1 cut(s) 492
MnlI CCTC 8 cut(s) 123, 174, 192, 313, 506, 580, 796, 814
Mox20I TGGCCA 1 cut(s) 492
MscI TGGCCA 1 cut(s) 492
MseI TTAA 3 cut(s) 162, 285, 761
MslI CAYNNNNRTG 1 cut(s) 794
Msp20I TGGCCA 1 cut(s) 492
MspI CCGG 1 cut(s) 437
MspR9I CCNGG 3 cut(s) 131, 231, 934
MunI CAATTG 1 cut(s) 168
MvaI CCWGG 3 cut(s) 131, 231, 934
MwoI GCNNNNNNNGC 5 cut(s) 276, 553, 587, 635, 728
NdeII GATC 3 cut(s) 138, 183, 871
NheI GCTAGC 2 cut(s) 428, 547
NlaIII CATG 5 cut(s) 68, 83, 539, 560, 793
NlaIV GGNNCC 3 cut(s) 185, 568, 814
NmuCI GTSAC 1 cut(s) 754
NspI RCATGY 2 cut(s) 83, 560
PciI ACATGT 1 cut(s) 79
PfoI TCCNGGA 1 cut(s) 229
PkrI GCNGC 6 cut(s) 154, 157, 373, 427, 442, 583
PscI ACATGT 1 cut(s) 79
PshBI ATTAAT 1 cut(s) 761
Psp1406I AACGTT 1 cut(s) 660
Psp6I CCWGG 3 cut(s) 129, 229, 932
PspFI CCCAGC 1 cut(s) 518
PspGI CCWGG 3 cut(s) 129, 229, 932
PspN4I GGNNCC 3 cut(s) 185, 568, 814
PspPI GGNCC 5 cut(s) 201, 767, 813, 881, 936
PstI CTGCAG 2 cut(s) 157, 376
PstNI CAGNNNCTG 2 cut(s) 136, 587
PsuI RGATCY 1 cut(s) 183
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
RseI CAYNNNNRTG 1 cut(s) 794
SaqAI TTAA 3 cut(s) 162, 285, 761
SatI GCNGC 6 cut(s) 153, 156, 372, 426, 441, 582
Sau3AI GATC 3 cut(s) 138, 183, 871
Sau96I GGNCC 5 cut(s) 201, 767, 813, 881, 936
ScrFI CCNGG 3 cut(s) 131, 231, 934
SduI GDGCHC 2 cut(s) 596, 801
SexAI ACCWGGT 1 cut(s) 129
SfaNI GCATC 2 cut(s) 163, 688
SfcI CTRYAG 3 cut(s) 153, 222, 372
SinI GGWCC 2 cut(s) 767, 813
SmiMI CAYNNNNRTG 1 cut(s) 794
SmlI CTYRAG 1 cut(s) 830
SmoI CTYRAG 1 cut(s) 830
Sse9I AATT 4 cut(s) 168, 281, 680, 890
SsiI CCGC 1 cut(s) 218
SspMI CTAG 2 cut(s) 429, 548
StyD4I CCNGG 3 cut(s) 129, 229, 932
StyI CCWWGG 1 cut(s) 187
TaiI ACGT 1 cut(s) 663
TasI AATT 4 cut(s) 168, 281, 680, 890
TatI WGTACW 1 cut(s) 363
Tru1I TTAA 3 cut(s) 162, 285, 761
Tru9I TTAA 3 cut(s) 162, 285, 761
TscAI CASTG 8 cut(s) 211, 217, 373, 592, 729, 738, 870, 946
TseFI GTSAC 1 cut(s) 754
TseI GCWGC 6 cut(s) 152, 155, 371, 425, 440, 581
Tsp45I GTSAC 1 cut(s) 754
TspDTI ATGAA 2 cut(s) 312, 474
TspRI CASTG 8 cut(s) 211, 217, 373, 592, 729, 738, 870, 946
VpaK11BI GGWCC 2 cut(s) 767, 813
VspI ATTAAT 1 cut(s) 761
XapI RAATTY 2 cut(s) 281, 680
XceI RCATGY 2 cut(s) 83, 560
XcmI CCANNNNNNNNNTGG 2 cut(s) 505, 946
XspI CTAG 2 cut(s) 429, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.