Prupe.3G284800_v2.0.a1

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001188
Physical Location & Seq
Forward (+)
25799212 .. 25800898
1687 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G284800.1

Sequence Viewer

Length: 975 bp
ATGGCCGATGCTTCATGTGTATCCACCACACCACCCTCTGTAAACAAGGCTTGGATTTACAGAGAGCATGGAGAAACTGCACATGTTTTGAAGTTTGAAACAAATGTGGCTGTTCCTCAAATAAAGGAAGACCAGGTGCTGATCAAGGTTGTTGCTGCAGCTCTTAACCCAATTGATGCCAAGAGGATCCTTGGCGTTTTCAGGGCCACTGACACTGCTCTGCCTACAGTTCCAGGGTTTGATGTGGCTGGTGTGGTGGTGAAGTTGGGAAGCAAAGCCAGCAAATTTAACATTGGGGATGAAGTGTATGGGGATATCAATGAGGAGGGTAGCATAAACCTGAAGAAGTTTGGGACTTTGGCAGAGTACACTGCTGCAGAAGAAAGATTGTTGGCTCTGAAACCCAAAAACCTGAGCTTTGTGGAAGCTGCTAGCCTTCCGGCGGCTATTGAGACAGCCTATGAAGGGCTGAAACGAGTTGGGCTTTCTGCTGGCCAGTCCATTCTTGTTTTGGGAGGCGCTGGGGGTTGTGGAACTCATGTTATTCAGCTAGCAAAGCATGTTTTTGGTGCCTCCAGAGTAGCAGCTACGGCGAGCACTAAAAAACTTGATTTGCTAAGAAGCTTGGGTGCTGATTTGGCTATTGACTACACTAAGGAAAACGTTGAAGACCTGCCTGAGAAATTTGATGTGGTGTATGATGCAGTTGGTCAATCGGATAAGGCAGTGAAGGCAGTGAAAGAAGGGGGAAGGGTGGTGACATTAATAGGTCCAATAACACCACCAGCCTCCATGTTTGTGCTCACCTCAACTGGGTCCATTTTGGAGAAACTCAAGCCTTACTTGGAGAGTAGGAAGGTGAAGGCAGTGATTGATCCCACAGGCCCGTATCCATTTTCTAAGACCATAGACGCATTTGCCTACCTTCAAACTTCCAGGGCTACTGGAAAGGTGGTTGTGTATCCCATCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

325

Amino Acids

34.34

Weight (kDa)

8.3

Isoelectric Point (pI)

27.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 681
AccB1I GGYRCC 1 cut(s) 569
AciI CCGC 1 cut(s) 443
AclI AACGTT 1 cut(s) 663
AclWI GGATC 3 cut(s) 181, 194, 869
AcoI YGGCCR 2 cut(s) 3, 493
AcsI RAATTY 2 cut(s) 284, 683
AcuI CTGAAG 1 cut(s) 362
AfaI GTAC 1 cut(s) 368
AfiI CCNNNNNNNGG 3 cut(s) 442, 465, 813
AflIII ACRYGT 1 cut(s) 82
AgsI TTSAA 4 cut(s) 91, 98, 668, 929
AjnI CCWGG 3 cut(s) 132, 232, 935
AluBI AGCT 6 cut(s) 161, 417, 428, 550, 587, 624
AluI AGCT 6 cut(s) 161, 417, 428, 550, 587, 624
Alw21I GWGCWC 2 cut(s) 599, 804
Alw26I GTCTC 1 cut(s) 446
AlwI GGATC 3 cut(s) 181, 194, 869
AlwNI CAGNNNCTG 1 cut(s) 139
AoxI GGCC 4 cut(s) 3, 204, 493, 883
ApeKI GCWGC 5 cut(s) 155, 158, 374, 428, 584
ApoI RAATTY 2 cut(s) 284, 683
AseI ATTAAT 1 cut(s) 764
AspLEI GCGC 1 cut(s) 521
AspS9I GGNCC 4 cut(s) 204, 770, 816, 884
AsuHPI GGTGA 4 cut(s) 271, 769, 796, 871
AsuNHI GCTAGC 2 cut(s) 431, 550
AvaII GGWCC 2 cut(s) 770, 816
BalI TGGCCA 1 cut(s) 495
BamHI GGATCC 1 cut(s) 186
BanI GGYRCC 1 cut(s) 569
BbsI GAAGAC 2 cut(s) 135, 675
Bbv12I GWGCWC 2 cut(s) 599, 804
BbvI GCAGC 5 cut(s) 142, 170, 361, 415, 596
BccI CCATC 1 cut(s) 974
BceAI ACGGC 1 cut(s) 606
BciT130I CCWGG 3 cut(s) 134, 234, 937
BciVI GTATCC 3 cut(s) 31, 900, 972
BclI TGATCA 1 cut(s) 141
BcoDI GTCTC 1 cut(s) 446
BfaI CTAG 2 cut(s) 432, 551
BfmI CTRYAG 3 cut(s) 156, 225, 375
BfoI RGCGCY 1 cut(s) 522
BfuAI ACCTGC 1 cut(s) 681
BfuI GTATCC 3 cut(s) 31, 900, 972
BisI GCNGC 6 cut(s) 156, 159, 375, 429, 444, 585
BlsI GCNGC 6 cut(s) 157, 160, 376, 430, 445, 586
Bme1390I CCNGG 3 cut(s) 134, 234, 937
Bme18I GGWCC 2 cut(s) 770, 816
BmgT120I GGNCC 4 cut(s) 204, 770, 816, 884
BmiI GGNNCC 3 cut(s) 188, 571, 817
BmrFI CCNGG 3 cut(s) 134, 234, 937
BmrI ACTGGG 1 cut(s) 822
BmsI GCATC 2 cut(s) 166, 691
BmtI GCTAGC 2 cut(s) 435, 554
BmuI ACTGGG 1 cut(s) 822
BpiI GAAGAC 2 cut(s) 135, 675
BpmI CTGGAG 1 cut(s) 559
Bpu10I CCTNAGC 1 cut(s) 413
BpuEI CTTGAG 1 cut(s) 818
BsaJI CCNNGG 3 cut(s) 190, 233, 936
Bsc4I CCNNNNNNNGG 3 cut(s) 442, 465, 813
Bse1I ACTGG 3 cut(s) 496, 817, 949
BseBI CCWGG 3 cut(s) 134, 234, 937
BseDI CCNNGG 3 cut(s) 190, 233, 936
BseGI GGATG 2 cut(s) 304, 966
BseLI CCNNNNNNNGG 3 cut(s) 442, 465, 813
BseMII CTCAG 2 cut(s) 404, 669
BseNI ACTGG 3 cut(s) 496, 817, 949
BseRI GAGGAG 1 cut(s) 338
BseXI GCAGC 5 cut(s) 142, 170, 361, 415, 596
BseYI CCCAGC 1 cut(s) 521
BsgI GTGCAG 1 cut(s) 63
BshFI GGCC 4 cut(s) 5, 206, 495, 885
BshNI GGYRCC 1 cut(s) 569
BsiHKAI GWGCWC 2 cut(s) 599, 804
BsiSI CCGG 1 cut(s) 440
BslFI GGGAC 1 cut(s) 367
BslI CCNNNNNNNGG 3 cut(s) 442, 465, 813
BsmAI GTCTC 1 cut(s) 446
BsmFI GGGAC 1 cut(s) 367
BsnI GGCC 4 cut(s) 5, 206, 495, 885
Bsp1286I GDGCHC 2 cut(s) 599, 804
Bsp143I GATC 3 cut(s) 141, 186, 874
BspACI CCGC 1 cut(s) 443
BspANI GGCC 4 cut(s) 5, 206, 495, 885
BspCNI CTCAG 2 cut(s) 405, 670
BspLI GGNNCC 3 cut(s) 188, 571, 817
BspMAI CTGCAG 2 cut(s) 160, 379
BspMI ACCTGC 1 cut(s) 681
BspOI GCTAGC 2 cut(s) 435, 554
BspPI GGATC 3 cut(s) 181, 194, 869
BspT107I GGYRCC 1 cut(s) 569
BsrI ACTGG 3 cut(s) 496, 817, 949
BssECI CCNNGG 3 cut(s) 190, 233, 936
BssMI GATC 3 cut(s) 141, 186, 874
BssT1I CCWWGG 1 cut(s) 190
Bst2UI CCWGG 3 cut(s) 134, 234, 937
Bst4CI ACNGT 1 cut(s) 229
BstC8I GCNNGC 5 cut(s) 280, 433, 493, 552, 595
BstDEI CTNAG 5 cut(s) 413, 617, 654, 678, 900
BstF5I GGATG 2 cut(s) 304, 966
BstH2I RGCGCY 1 cut(s) 522
BstHHI GCGC 1 cut(s) 521
BstKTI GATC 3 cut(s) 144, 189, 877
BstMAI GTCTC 1 cut(s) 446
BstMBI GATC 3 cut(s) 141, 186, 874
BstMWI GCNNNNNNNGC 5 cut(s) 279, 556, 590, 638, 731
BstNI CCWGG 3 cut(s) 134, 234, 937
BstNSI RCATGY 2 cut(s) 86, 563
BstSCI CCNGG 3 cut(s) 132, 232, 935
BstSFI CTRYAG 3 cut(s) 156, 225, 375
BstV1I GCAGC 5 cut(s) 142, 170, 361, 415, 596
BstV2I GAAGAC 2 cut(s) 135, 675
BstX2I RGATCY 1 cut(s) 186
BstYI RGATCY 1 cut(s) 186
BsuI GTATCC 3 cut(s) 31, 900, 972
BsuRI GGCC 4 cut(s) 5, 206, 495, 885
BtsCI GGATG 2 cut(s) 304, 966
BtsI GCAGTG 5 cut(s) 213, 369, 732, 741, 873
BtsIMutI CAGTG 6 cut(s) 207, 213, 369, 732, 741, 873
BveI ACCTGC 1 cut(s) 681
Cac8I GCNNGC 5 cut(s) 280, 433, 493, 552, 595
CaiI CAGNNNCTG 1 cut(s) 139
CfoI GCGC 1 cut(s) 521
Cfr13I GGNCC 4 cut(s) 204, 770, 816, 884
CseI GACGC 1 cut(s) 920
CsiI ACCWGGT 1 cut(s) 132
Csp6I GTAC 1 cut(s) 367
CviAII CATG 6 cut(s) 15, 68, 83, 539, 560, 793
CviQI GTAC 1 cut(s) 367
DdeI CTNAG 5 cut(s) 413, 617, 654, 678, 900
DpnI GATC 3 cut(s) 143, 188, 876
DpnII GATC 3 cut(s) 141, 186, 874
EaeI YGGCCR 2 cut(s) 3, 493
Eco130I CCWWGG 1 cut(s) 190
Eco32I GATATC 1 cut(s) 316
Eco47I GGWCC 2 cut(s) 770, 816
Eco57I CTGAAG 1 cut(s) 362
EcoRII CCWGG 3 cut(s) 132, 232, 935
EcoRV GATATC 1 cut(s) 316
EcoT14I CCWWGG 1 cut(s) 190
ErhI CCWWGG 1 cut(s) 190
FaeI CATG 6 cut(s) 18, 71, 86, 542, 563, 796
FalI AAGNNNNNCTT 2 cut(s) 827, 859
FaqI GGGAC 1 cut(s) 367
FatI CATG 6 cut(s) 14, 67, 82, 538, 559, 792
FbaI TGATCA 1 cut(s) 141
Fnu4HI GCNGC 6 cut(s) 156, 159, 375, 429, 444, 585
FokI GGATG 2 cut(s) 311, 953
Fsp4HI GCNGC 6 cut(s) 156, 159, 375, 429, 444, 585
FspBI CTAG 2 cut(s) 432, 551
GlaI GCGC 1 cut(s) 520
GluI GCNGC 6 cut(s) 156, 159, 375, 429, 444, 585
GsaI CCCAGC 1 cut(s) 525
GsuI CTGGAG 1 cut(s) 559
HaeII RGCGCY 1 cut(s) 522
HaeIII GGCC 4 cut(s) 5, 206, 495, 885
HapII CCGG 1 cut(s) 440
HgaI GACGC 1 cut(s) 920
HhaI GCGC 1 cut(s) 521
Hin1II CATG 6 cut(s) 18, 71, 86, 542, 563, 796
Hin6I GCGC 1 cut(s) 519
HinP1I GCGC 1 cut(s) 519
HindIII AAGCTT 1 cut(s) 622
HpaII CCGG 1 cut(s) 440
HphI GGTGA 4 cut(s) 271, 769, 796, 871
Hpy166II GTNNAC 2 cut(s) 43, 369
Hpy188I TCNGA 2 cut(s) 399, 718
Hpy188III TCNNGA 1 cut(s) 576
Hpy8I GTNNAC 2 cut(s) 43, 369
HpyAV CCTTC 8 cut(s) 446, 458, 724, 737, 744, 850, 856, 935
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4IV ACGT 1 cut(s) 663
HpyCH4V TGCA 4 cut(s) 80, 158, 377, 704
HpyF10VI GCNNNNNNNGC 5 cut(s) 279, 556, 590, 638, 731
HpyF3I CTNAG 5 cut(s) 413, 617, 654, 678, 900
HpySE526I ACGT 1 cut(s) 663
Hsp92II CATG 6 cut(s) 18, 71, 86, 542, 563, 796
HspAI GCGC 1 cut(s) 519
Ksp22I TGATCA 1 cut(s) 141
Kzo9I GATC 3 cut(s) 141, 186, 874
Lsp1109I GCAGC 5 cut(s) 142, 170, 361, 415, 596
LweI GCATC 2 cut(s) 166, 691
MabI ACCWGGT 1 cut(s) 132
MaeI CTAG 2 cut(s) 432, 551
MaeII ACGT 1 cut(s) 663
MaeIII GTNAC 1 cut(s) 757
MalI GATC 3 cut(s) 143, 188, 876
MboI GATC 3 cut(s) 141, 186, 874
MboII GAAGA 4 cut(s) 140, 355, 392, 680
MfeI CAATTG 1 cut(s) 171
MflI RGATCY 1 cut(s) 186
MhlI GDGCHC 2 cut(s) 599, 804
MlsI TGGCCA 1 cut(s) 495
MluCI AATT 3 cut(s) 171, 284, 683
MluNI TGGCCA 1 cut(s) 495
MnlI CCTC 9 cut(s) 46, 126, 177, 316, 319, 509, 583, 799, 817
Mox20I TGGCCA 1 cut(s) 495
MscI TGGCCA 1 cut(s) 495
MseI TTAA 3 cut(s) 165, 288, 764
MslI CAYNNNNRTG 1 cut(s) 797
Msp20I TGGCCA 1 cut(s) 495
MspI CCGG 1 cut(s) 440
MspR9I CCNGG 3 cut(s) 134, 234, 937
MunI CAATTG 1 cut(s) 171
MvaI CCWGG 3 cut(s) 134, 234, 937
MwoI GCNNNNNNNGC 5 cut(s) 279, 556, 590, 638, 731
NdeII GATC 3 cut(s) 141, 186, 874
NheI GCTAGC 2 cut(s) 431, 550
NlaIII CATG 6 cut(s) 18, 71, 86, 542, 563, 796
NlaIV GGNNCC 3 cut(s) 188, 571, 817
NmuCI GTSAC 1 cut(s) 757
NspI RCATGY 2 cut(s) 86, 563
PciI ACATGT 1 cut(s) 82
PkrI GCNGC 6 cut(s) 157, 160, 376, 430, 445, 586
PscI ACATGT 1 cut(s) 82
PshBI ATTAAT 1 cut(s) 764
Psp1406I AACGTT 1 cut(s) 663
Psp6I CCWGG 3 cut(s) 132, 232, 935
PspFI CCCAGC 1 cut(s) 521
PspGI CCWGG 3 cut(s) 132, 232, 935
PspN4I GGNNCC 3 cut(s) 188, 571, 817
PspPI GGNCC 4 cut(s) 204, 770, 816, 884
PstI CTGCAG 2 cut(s) 160, 379
PstNI CAGNNNCTG 1 cut(s) 139
PsuI RGATCY 1 cut(s) 186
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
RseI CAYNNNNRTG 1 cut(s) 797
SaqAI TTAA 3 cut(s) 165, 288, 764
SatI GCNGC 6 cut(s) 156, 159, 375, 429, 444, 585
Sau3AI GATC 3 cut(s) 141, 186, 874
Sau96I GGNCC 4 cut(s) 204, 770, 816, 884
ScrFI CCNGG 3 cut(s) 134, 234, 937
SduI GDGCHC 2 cut(s) 599, 804
SexAI ACCWGGT 1 cut(s) 132
SfaNI GCATC 2 cut(s) 166, 691
SfcI CTRYAG 3 cut(s) 156, 225, 375
SinI GGWCC 2 cut(s) 770, 816
SmiMI CAYNNNNRTG 1 cut(s) 797
SmlI CTYRAG 1 cut(s) 833
SmoI CTYRAG 1 cut(s) 833
Sse9I AATT 3 cut(s) 171, 284, 683
SsiI CCGC 1 cut(s) 443
SspMI CTAG 2 cut(s) 432, 551
StyD4I CCNGG 3 cut(s) 132, 232, 935
StyI CCWWGG 1 cut(s) 190
TaaI ACNGT 1 cut(s) 229
TaiI ACGT 1 cut(s) 666
TasI AATT 3 cut(s) 171, 284, 683
TatI WGTACW 1 cut(s) 366
TauI GCSGC 1 cut(s) 446
Tru1I TTAA 3 cut(s) 165, 288, 764
Tru9I TTAA 3 cut(s) 165, 288, 764
TscAI CASTG 6 cut(s) 214, 220, 376, 732, 741, 873
TseFI GTSAC 1 cut(s) 757
TseI GCWGC 5 cut(s) 155, 158, 374, 428, 584
Tsp45I GTSAC 1 cut(s) 757
TspDTI ATGAA 2 cut(s) 315, 477
TspRI CASTG 6 cut(s) 214, 220, 376, 732, 741, 873
VpaK11BI GGWCC 2 cut(s) 770, 816
VspI ATTAAT 1 cut(s) 764
XapI RAATTY 2 cut(s) 284, 683
XceI RCATGY 2 cut(s) 86, 563
XcmI CCANNNNNNNNNTGG 1 cut(s) 508
XspI CTAG 2 cut(s) 432, 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.