MD09G1034000.v1.1

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001188
Physical Location & Seq
Reverse (-)
2082430 .. 2084487
2058 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1034000.v1.1.491

Sequence Viewer

Length: 984 bp
ATGGCGGCTGCTGTAGCTTCAAGTGATTCAGTTTCCATACCATCTGTAAACAAGGCGTGGGTGTACCCAGAGTACGGAAGGTCTGCAGATGTTTTGAAGTTCGATCCGAATGTTCCTGTTCCTGAAATAAAGGAAGACCAGGTGCTGATCAAGGTGGTTGCTGCATCTCTCAACCCAGTTGATTTCAAAAGGATGCTTGGCTACTTCAAGGACTATGACTCTTCACCCCCTACAGTTCCAGGGTATGATGTTGCTGGTGTGGTGGTGAAAGTAGGGAGCCAGGTGACAAAGTTTAAGGTGGGGGAGGATGAAGTATATGGGGATCTCAACGAGAAGCCTCTCACCAAGCCGAAAAGGATCGGATCTTTGGCCGAGTACACTGCTGTAGAAGAAAGAGTGTTGGCTCTCAAGCCCAAAAATCTGAGTTTTGTTGAAGCTGCTAGCCTTCCCCTGGCCATTGAGACTGCCTATGAAGGACTCGAACGACTTGAACTCTCTGCCGGTAAATCCATCCTTGTTTTGGGAGGCGCTGGGGGAGTCGGAACACATGTTATTCAGCTAGCAAAACATGTGTTTGGTGCTTCAAAAGTAGCAGCTACAGCGAGCACGAAAAAACTCGATCTTTTGAGAAGCTTGGGTGCTGATTTGGCTATTGATTACACCAAGGAGAACTTCGAAGACCTGACCGAGAAATTTGATGTGGTTTATAATGCAGTTGGGGAGACTGACAGGGCAGTGAAGGCGGTGAAGGAAGGCGGGAAGGTATTGACAATTGTACGTCCAGTAGCGCCACCGGCATTCCTATTTTTGCTGACCTCAACCGGAACTGTATTAGAGAAACTGAAGCCTTACTTGGAGAGTGGGAAGGTGAACCCGGTGCTTGATCCCAGCGGCCCATATCCATTTTCGAAGACTGTTGAAGCATTTGCCTATCTTGAAACCTCCAGAGCTCCTGGAAAGGTGGTTGTGTATCCCATCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

328

Amino Acids

35.03

Weight (kDa)

5.91

Isoelectric Point (pI)

26.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 45 - 138 6.2e-15 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 179 - 258 1.5e-13 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 212 - 323 1.7e-18 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 708
AciI CCGC 4 cut(s) 5, 743, 756, 891
AclWI GGATC 5 cut(s) 98, 330, 365, 370, 878
AcoI YGGCCR 2 cut(s) 369, 453
AcsI RAATTY 1 cut(s) 692
AcuI CTGAAG 1 cut(s) 863
AfaI GTAC 4 cut(s) 65, 74, 377, 777
AfiI CCNNNNNNNGG 3 cut(s) 74, 451, 520
AflIII ACRYGT 2 cut(s) 547, 568
AgsI TTSAA 9 cut(s) 21, 97, 187, 208, 434, 491, 585, 920, 938
AjnI CCWGG 5 cut(s) 138, 238, 279, 450, 952
AjuI GAANNNNNNNTTGG 4 cut(s) 656, 688, 836, 868
AluBI AGCT 6 cut(s) 17, 437, 559, 596, 633, 950
AluI AGCT 6 cut(s) 17, 437, 559, 596, 633, 950
Alw21I GWGCWC 2 cut(s) 608, 952
Alw26I GTCTC 2 cut(s) 455, 716
AlwI GGATC 5 cut(s) 98, 330, 365, 370, 878
AlwNI CAGNNNCTG 1 cut(s) 145
AoxI GGCC 3 cut(s) 369, 453, 892
ApeKI GCWGC 4 cut(s) 8, 161, 437, 593
ApoI RAATTY 1 cut(s) 692
AspLEI GCGC 2 cut(s) 530, 790
AspS9I GGNCC 1 cut(s) 893
AsuC2I CCSGG 1 cut(s) 875
AsuHPI GGTGA 6 cut(s) 216, 277, 295, 334, 757, 880
AsuII TTCGAA 2 cut(s) 675, 908
AsuNHI GCTAGC 2 cut(s) 440, 559
BalI TGGCCA 1 cut(s) 455
BanII GRGCYC 1 cut(s) 952
BbsI GAAGAC 3 cut(s) 141, 684, 917
Bbv12I GWGCWC 2 cut(s) 608, 952
BbvI GCAGC 3 cut(s) 148, 424, 605
BccI CCATC 3 cut(s) 49, 518, 983
BcgI CGANNNNNNTGC 2 cut(s) 362, 396
BciT130I CCWGG 5 cut(s) 140, 240, 281, 452, 954
BciVI GTATCC 1 cut(s) 981
BclI TGATCA 1 cut(s) 147
BcnI CCSGG 1 cut(s) 875
BcoDI GTCTC 2 cut(s) 455, 716
BfaI CTAG 2 cut(s) 441, 560
BfmI CTRYAG 5 cut(s) 12, 84, 231, 384, 597
BfoI RGCGCY 2 cut(s) 531, 791
BfuI GTATCC 1 cut(s) 981
BisI GCNGC 6 cut(s) 6, 9, 162, 438, 594, 892
BlsI GCNGC 6 cut(s) 7, 10, 163, 439, 595, 893
Bme1390I CCNGG 6 cut(s) 140, 240, 281, 452, 875, 954
BmgT120I GGNCC 1 cut(s) 893
BmiI GGNNCC 1 cut(s) 278
BmrFI CCNGG 6 cut(s) 140, 240, 281, 452, 875, 954
BmrI ACTGGG 1 cut(s) 170
BmsI GCATC 2 cut(s) 173, 183
BmtI GCTAGC 2 cut(s) 444, 563
BmuI ACTGGG 1 cut(s) 170
BpiI GAAGAC 3 cut(s) 141, 684, 917
BpmI CTGGAG 1 cut(s) 928
Bpu14I TTCGAA 2 cut(s) 675, 908
BpuEI CTTGAG 1 cut(s) 392
BpuMI CCSGG 1 cut(s) 875
BsaJI CCNNGG 3 cut(s) 239, 450, 663
BsaWI WCCGGW 1 cut(s) 821
Bsc4I CCNNNNNNNGG 3 cut(s) 74, 451, 520
Bse118I RCCGGY 2 cut(s) 500, 793
Bse1I ACTGG 2 cut(s) 176, 782
BseBI CCWGG 5 cut(s) 140, 240, 281, 452, 954
BseDI CCNNGG 3 cut(s) 239, 450, 663
BseGI GGATG 4 cut(s) 198, 313, 510, 975
BseLI CCNNNNNNNGG 3 cut(s) 74, 451, 520
BseMII CTCAG 1 cut(s) 413
BseNI ACTGG 2 cut(s) 176, 782
BseXI GCAGC 3 cut(s) 148, 424, 605
BseYI CCCAGC 2 cut(s) 530, 887
BshFI GGCC 3 cut(s) 371, 455, 894
BsiHKAI GWGCWC 2 cut(s) 608, 952
BsiSI CCGG 4 cut(s) 501, 794, 822, 875
BslI CCNNNNNNNGG 3 cut(s) 74, 451, 520
BsmAI GTCTC 2 cut(s) 455, 716
BsmI GAATGC 1 cut(s) 797
BsnI GGCC 3 cut(s) 371, 455, 894
Bsp119I TTCGAA 2 cut(s) 675, 908
Bsp1286I GDGCHC 2 cut(s) 608, 952
Bsp143I GATC 7 cut(s) 103, 147, 322, 357, 362, 619, 883
BspACI CCGC 4 cut(s) 5, 743, 756, 891
BspANI GGCC 3 cut(s) 371, 455, 894
BspCNI CTCAG 1 cut(s) 414
BspLI GGNNCC 1 cut(s) 278
BspMAI CTGCAG 1 cut(s) 88
BspOI GCTAGC 2 cut(s) 444, 563
BspPI GGATC 5 cut(s) 98, 330, 365, 370, 878
BspT104I TTCGAA 2 cut(s) 675, 908
BsrFI RCCGGY 2 cut(s) 500, 793
BsrI ACTGG 2 cut(s) 176, 782
BssAI RCCGGY 2 cut(s) 500, 793
BssECI CCNNGG 3 cut(s) 239, 450, 663
BssMI GATC 7 cut(s) 103, 147, 322, 357, 362, 619, 883
BssT1I CCWWGG 1 cut(s) 663
Bst2UI CCWGG 5 cut(s) 140, 240, 281, 452, 954
Bst4CI ACNGT 3 cut(s) 235, 829, 916
Bst6I CTCTTC 1 cut(s) 226
BstBI TTCGAA 2 cut(s) 675, 908
BstC8I GCNNGC 3 cut(s) 442, 561, 604
BstDEI CTNAG 1 cut(s) 422
BstF5I GGATG 4 cut(s) 198, 313, 510, 975
BstH2I RGCGCY 2 cut(s) 531, 791
BstHHI GCGC 2 cut(s) 530, 790
BstKTI GATC 7 cut(s) 106, 150, 325, 360, 365, 622, 886
BstMAI GTCTC 2 cut(s) 455, 716
BstMBI GATC 7 cut(s) 103, 147, 322, 357, 362, 619, 883
BstMWI GCNNNNNNNGC 5 cut(s) 14, 599, 647, 740, 794
BstNI CCWGG 5 cut(s) 140, 240, 281, 452, 954
BstNSI RCATGY 2 cut(s) 551, 572
BstSCI CCNGG 6 cut(s) 138, 238, 279, 450, 873, 952
BstSFI CTRYAG 5 cut(s) 12, 84, 231, 384, 597
BstV1I GCAGC 3 cut(s) 148, 424, 605
BstV2I GAAGAC 3 cut(s) 141, 684, 917
BstX2I RGATCY 2 cut(s) 322, 362
BstYI RGATCY 2 cut(s) 322, 362
BsuI GTATCC 1 cut(s) 981
BsuRI GGCC 3 cut(s) 371, 455, 894
BtsCI GGATG 4 cut(s) 198, 313, 510, 975
BtsI GCAGTG 2 cut(s) 378, 741
BtsIMutI CAGTG 2 cut(s) 378, 741
Cac8I GCNNGC 3 cut(s) 442, 561, 604
CaiI CAGNNNCTG 1 cut(s) 145
CfoI GCGC 2 cut(s) 530, 790
Cfr10I RCCGGY 2 cut(s) 500, 793
Cfr13I GGNCC 1 cut(s) 893
CsiI ACCWGGT 1 cut(s) 138
Csp6I GTAC 4 cut(s) 64, 73, 376, 776
CviAII CATG 3 cut(s) 548, 569, 981
CviQI GTAC 4 cut(s) 64, 73, 376, 776
DdeI CTNAG 1 cut(s) 422
DpnI GATC 7 cut(s) 105, 149, 324, 359, 364, 621, 885
DpnII GATC 7 cut(s) 103, 147, 322, 357, 362, 619, 883
EaeI YGGCCR 2 cut(s) 369, 453
Eam1104I CTCTTC 1 cut(s) 226
EarI CTCTTC 1 cut(s) 226
Ecl136II GAGCTC 1 cut(s) 950
Eco130I CCWWGG 1 cut(s) 663
Eco24I GRGCYC 1 cut(s) 952
Eco53kI GAGCTC 1 cut(s) 950
Eco57I CTGAAG 1 cut(s) 863
EcoICRI GAGCTC 1 cut(s) 950
EcoRII CCWGG 5 cut(s) 138, 238, 279, 450, 952
EcoT14I CCWWGG 1 cut(s) 663
EcoT38I GRGCYC 1 cut(s) 952
ErhI CCWWGG 1 cut(s) 663
FaeI CATG 3 cut(s) 551, 572, 984
FalI AAGNNNNNCTT 4 cut(s) 656, 688, 836, 868
FatI CATG 3 cut(s) 547, 568, 980
FauI CCCGC 1 cut(s) 749
FbaI TGATCA 1 cut(s) 147
Fnu4HI GCNGC 6 cut(s) 6, 9, 162, 438, 594, 892
FokI GGATG 4 cut(s) 205, 320, 497, 962
FriOI GRGCYC 1 cut(s) 952
Fsp4HI GCNGC 6 cut(s) 6, 9, 162, 438, 594, 892
FspBI CTAG 2 cut(s) 441, 560
GlaI GCGC 2 cut(s) 529, 789
GluI GCNGC 6 cut(s) 6, 9, 162, 438, 594, 892
GsaI CCCAGC 2 cut(s) 534, 891
GsuI CTGGAG 1 cut(s) 928
HaeII RGCGCY 2 cut(s) 531, 791
HaeIII GGCC 3 cut(s) 371, 455, 894
HapII CCGG 4 cut(s) 501, 794, 822, 875
HhaI GCGC 2 cut(s) 530, 790
Hin1II CATG 3 cut(s) 551, 572, 984
Hin6I GCGC 2 cut(s) 528, 788
HinP1I GCGC 2 cut(s) 528, 788
HindIII AAGCTT 1 cut(s) 631
HinfI GANTC 4 cut(s) 26, 218, 477, 537
HpaII CCGG 4 cut(s) 501, 794, 822, 875
HphI GGTGA 6 cut(s) 216, 277, 295, 334, 757, 880
Hpy166II GTNNAC 4 cut(s) 49, 64, 378, 871
Hpy188I TCNGA 4 cut(s) 108, 362, 423, 542
Hpy188III TCNNGA 3 cut(s) 122, 935, 945
Hpy8I GTNNAC 4 cut(s) 49, 64, 378, 871
HpyAV CCTTC 8 cut(s) 72, 455, 467, 733, 742, 746, 754, 859
HpyCH4III ACNGT 3 cut(s) 235, 829, 916
HpyCH4IV ACGT 1 cut(s) 778
HpyCH4V TGCA 3 cut(s) 86, 164, 713
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 599, 647, 740, 794
HpyF3I CTNAG 1 cut(s) 422
HpySE526I ACGT 1 cut(s) 778
Hsp92II CATG 3 cut(s) 551, 572, 984
HspAI GCGC 2 cut(s) 528, 788
Ksp22I TGATCA 1 cut(s) 147
Kzo9I GATC 7 cut(s) 103, 147, 322, 357, 362, 619, 883
LmnI GCTCC 2 cut(s) 276, 955
Lsp1109I GCAGC 3 cut(s) 148, 424, 605
LweI GCATC 2 cut(s) 173, 183
MabI ACCWGGT 1 cut(s) 138
MaeI CTAG 2 cut(s) 441, 560
MaeII ACGT 1 cut(s) 778
MaeIII GTNAC 1 cut(s) 283
MalI GATC 7 cut(s) 105, 149, 324, 359, 364, 621, 885
MboI GATC 7 cut(s) 103, 147, 322, 357, 362, 619, 883
MboII GAAGA 5 cut(s) 146, 213, 401, 689, 922
MfeI CAATTG 1 cut(s) 771
MflI RGATCY 2 cut(s) 322, 362
MhlI GDGCHC 2 cut(s) 608, 952
MlsI TGGCCA 1 cut(s) 455
MluCI AATT 2 cut(s) 692, 771
MluNI TGGCCA 1 cut(s) 455
MlyI GAGTC 3 cut(s) 212, 471, 546
MmeI TCCRAC 1 cut(s) 520
MnlI CCTC 5 cut(s) 298, 348, 518, 826, 952
Mox20I TGGCCA 1 cut(s) 455
MscI TGGCCA 1 cut(s) 455
MseI TTAA 1 cut(s) 294
Msp20I TGGCCA 1 cut(s) 455
MspA1I CMGCKG 1 cut(s) 891
MspI CCGG 4 cut(s) 501, 794, 822, 875
MspR9I CCNGG 6 cut(s) 140, 240, 281, 452, 875, 954
MunI CAATTG 1 cut(s) 771
Mva1269I GAATGC 1 cut(s) 797
MvaI CCWGG 5 cut(s) 140, 240, 281, 452, 954
MwoI GCNNNNNNNGC 5 cut(s) 14, 599, 647, 740, 794
NciI CCSGG 1 cut(s) 875
NdeII GATC 7 cut(s) 103, 147, 322, 357, 362, 619, 883
NheI GCTAGC 2 cut(s) 440, 559
NlaIII CATG 3 cut(s) 551, 572, 984
NlaIV GGNNCC 1 cut(s) 278
NmeAIII GCCGAG 1 cut(s) 397
NmuCI GTSAC 1 cut(s) 283
NspI RCATGY 2 cut(s) 551, 572
NspV TTCGAA 2 cut(s) 675, 908
PciI ACATGT 2 cut(s) 547, 568
PctI GAATGC 1 cut(s) 797
PfeI GAWTC 1 cut(s) 26
PfoI TCCNGGA 1 cut(s) 952
PkrI GCNGC 6 cut(s) 7, 10, 163, 439, 595, 893
PleI GAGTC 3 cut(s) 212, 471, 545
PpsI GAGTC 3 cut(s) 212, 471, 545
PscI ACATGT 2 cut(s) 547, 568
PsiI TTATAA 1 cut(s) 708
Psp124BI GAGCTC 1 cut(s) 952
Psp6I CCWGG 5 cut(s) 138, 238, 279, 450, 952
PspFI CCCAGC 2 cut(s) 530, 887
PspGI CCWGG 5 cut(s) 138, 238, 279, 450, 952
PspN4I GGNNCC 1 cut(s) 278
PspPI GGNCC 1 cut(s) 893
PstI CTGCAG 1 cut(s) 88
PstNI CAGNNNCTG 1 cut(s) 145
PsuI RGATCY 2 cut(s) 322, 362
RsaI GTAC 4 cut(s) 65, 74, 377, 777
RsaNI GTAC 4 cut(s) 64, 73, 376, 776
SacI GAGCTC 1 cut(s) 952
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 6 cut(s) 6, 9, 162, 438, 594, 892
Sau3AI GATC 7 cut(s) 103, 147, 322, 357, 362, 619, 883
Sau96I GGNCC 1 cut(s) 893
SchI GAGTC 3 cut(s) 212, 471, 546
ScrFI CCNGG 6 cut(s) 140, 240, 281, 452, 875, 954
SduI GDGCHC 2 cut(s) 608, 952
SexAI ACCWGGT 1 cut(s) 138
SfaNI GCATC 2 cut(s) 173, 183
SfcI CTRYAG 5 cut(s) 12, 84, 231, 384, 597
SfuI TTCGAA 2 cut(s) 675, 908
SmlI CTYRAG 1 cut(s) 407
SmoI CTYRAG 1 cut(s) 407
Sse9I AATT 2 cut(s) 692, 771
SsiI CCGC 4 cut(s) 5, 743, 756, 891
SspMI CTAG 2 cut(s) 441, 560
SstI GAGCTC 1 cut(s) 952
StyD4I CCNGG 6 cut(s) 138, 238, 279, 450, 873, 952
StyI CCWWGG 1 cut(s) 663
TaaI ACNGT 3 cut(s) 235, 829, 916
TaiI ACGT 1 cut(s) 781
TaqI TCGA 5 cut(s) 102, 480, 618, 675, 908
TaqII GACCGA 1 cut(s) 701
TasI AATT 2 cut(s) 692, 771
TatI WGTACW 1 cut(s) 375
TauI GCSGC 2 cut(s) 8, 894
TfiI GAWTC 1 cut(s) 26
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 2 cut(s) 385, 741
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 4 cut(s) 8, 161, 437, 593
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 2 cut(s) 324, 486
TspGWI ACGGA 1 cut(s) 90
TspRI CASTG 2 cut(s) 385, 741
XapI RAATTY 1 cut(s) 692
XceI RCATGY 2 cut(s) 551, 572
XcmI CCANNNNNNNNNTGG 1 cut(s) 517
XspI CTAG 2 cut(s) 441, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.