RLG00000022020

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001188
Physical Location & Seq
Forward (+)
80021833 .. 80023729
1897 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022020

Sequence Viewer

Length: 1032 bp
ATGGCTGCAGCTCCAAGTGAGTGCATACCCTCTGTATATGGGGCTTGGGTCTATTCAGAGTATGGAAAAACTGCAGATGTTCTGAAATTTGATCCAAGTGTGGCTGTTCCTGAAATTAAAGAGGACCAGGTGCTGATCAAGGTGGTTGCTGCTTCTCTTAACCCAGTTGATTTGAGAAGGGCTCTTGGTCTCTACAAGGACATAGAGGGTGATACTACCTTACCTACAATTCCAGGGTATGATGTGGCAGGTGTGGTGGTGAAGGTAGGAAGCCAAGTGCAGAAGTTTAAGGTGGGGGATGAAGTGTATGCTTGTTTGAGTAATAGCATTGAGATTCCCCAATATCATGCTAAGAACAGCCATGGGGATCTCAATGAGAAAGCATTGGTGAAACCAAACAGGCTGGGGTCTTTGGCAAAGTACACTGCTGCAGGAGAAACCATGTTGGCTCTCAAACCCAAAAATCTGAGCTTTATTGAAGCTGCTAGCCTTCCCTTAGCTATTGAGACTGCTTATGGAGCGCTTGAAAGAGCGGAGCTTTCGGCTGGTAAATCCGTCCTTGTTTTGGGAGGCGCTGGGGGTGTTGGAACACATGTGATTCAGCTAGCTAAACATGTTTTTGGGGCATCCAAAGTAGCAGCTACTGCAAGCACTAAAAAACTGGATTTGTTAAGAAGCTTGGGTGCGGATTTGGCTATTGATTACACCAAGGAAAACATCGAAGACCTGCCAGAGAAATTTGATGTAGTCTATGATGCGGTTGGGCAGACTGACAAGGCAGTGAAGGCGGTGAAGGAAGGTGGGAAGGTAGTGACAATAGTCATAGGTCCAGTAACTCCTCCGGCGTTCAGATTTGTGCTCACCCCTACAGGGTCTATCTTGGAGAAACTGAAGCCTTACTTGGAGAGCGGGAAGGTGAAGCCAATGCTTGATCCCATAGGTCCATATCCCTTTTCTAAAGTTGTTGAAGCACTTGGTTACCTTGGGACTTCTAGAGCTACAGGAAAGGTGGTTGTGTACCCTATCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

344

Amino Acids

36.29

Weight (kDa)

7.64

Isoelectric Point (pI)

27.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 41 - 153 1.7e-16 Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C PF16912 167 - 267 3e-06 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 194 - 275 6.7e-15 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 227 - 339 3.7e-17 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 239
Acc36I ACCTGC 2 cut(s) 239, 735
AccBSI CCGCTC 2 cut(s) 533, 909
AciI CCGC 5 cut(s) 533, 686, 758, 788, 909
AclWI GGATC 3 cut(s) 86, 375, 926
AcsI RAATTY 2 cut(s) 86, 737
AcuI CTGAAG 1 cut(s) 911
AfaI GTAC 2 cut(s) 422, 1019
AfeI AGCGCT 1 cut(s) 522
AfiI CCNNNNNNNGG 2 cut(s) 565, 870
AflIII ACRYGT 2 cut(s) 592, 613
AgsI TTSAA 3 cut(s) 479, 527, 968
AjnI CCWGG 2 cut(s) 126, 232
AjuI GAANNNNNNNTTGG 2 cut(s) 884, 916
Alw21I GWGCWC 1 cut(s) 861
Alw26I GTCTC 2 cut(s) 194, 500
AlwI GGATC 3 cut(s) 86, 375, 926
AlwNI CAGNNNCTG 2 cut(s) 133, 644
Aor51HI AGCGCT 1 cut(s) 522
ApeKI GCWGC 6 cut(s) 5, 8, 149, 428, 482, 638
ApoI RAATTY 2 cut(s) 86, 737
AspLEI GCGC 2 cut(s) 523, 575
AspS9I GGNCC 3 cut(s) 124, 827, 941
AsuHPI GGTGA 6 cut(s) 221, 271, 400, 802, 853, 928
AsuNHI GCTAGC 2 cut(s) 485, 604
AvaII GGWCC 3 cut(s) 124, 827, 941
BanII GRGCYC 1 cut(s) 184
BbsI GAAGAC 1 cut(s) 729
Bbv12I GWGCWC 1 cut(s) 861
BbvI GCAGC 5 cut(s) 20, 136, 415, 469, 650
BciT130I CCWGG 2 cut(s) 128, 234
BclI TGATCA 1 cut(s) 135
BcoDI GTCTC 2 cut(s) 194, 500
BfaI CTAG 3 cut(s) 486, 605, 993
BfmI CTRYAG 5 cut(s) 6, 72, 429, 867, 999
BfoI RGCGCY 2 cut(s) 524, 576
BfuAI ACCTGC 2 cut(s) 239, 735
BisI GCNGC 6 cut(s) 6, 9, 150, 429, 483, 639
BlsI GCNGC 6 cut(s) 7, 10, 151, 430, 484, 640
Bme1390I CCNGG 2 cut(s) 128, 234
Bme18I GGWCC 3 cut(s) 124, 827, 941
BmgT120I GGNCC 3 cut(s) 124, 827, 941
BmrFI CCNGG 2 cut(s) 128, 234
BmrI ACTGGG 1 cut(s) 158
BmsI GCATC 2 cut(s) 635, 745
BmtI GCTAGC 2 cut(s) 489, 608
BmuI ACTGGG 1 cut(s) 158
BoxI GACNNNNGTC 1 cut(s) 818
BpiI GAAGAC 1 cut(s) 729
BplI GAGNNNNNCTC 2 cut(s) 166, 198
Bpu10I CCTNAGC 1 cut(s) 496
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 4 cut(s) 233, 361, 708, 982
Bsc4I CCNNNNNNNGG 2 cut(s) 565, 870
Bse1I ACTGG 3 cut(s) 164, 666, 830
BseBI CCWGG 2 cut(s) 128, 234
BseDI CCNNGG 4 cut(s) 233, 361, 708, 982
BseGI GGATG 2 cut(s) 304, 626
BseLI CCNNNNNNNGG 2 cut(s) 565, 870
BseMII CTCAG 1 cut(s) 458
BseNI ACTGG 3 cut(s) 164, 666, 830
BseRI GAGGAG 1 cut(s) 828
BseXI GCAGC 5 cut(s) 20, 136, 415, 469, 650
BseYI CCCAGC 2 cut(s) 403, 575
BsgI GTGCAG 1 cut(s) 299
BsiHKAI GWGCWC 1 cut(s) 861
BsiSI CCGG 1 cut(s) 842
BslFI GGGAC 1 cut(s) 1000
BslI CCNNNNNNNGG 2 cut(s) 565, 870
BsmAI GTCTC 2 cut(s) 194, 500
BsmFI GGGAC 1 cut(s) 1000
Bso31I GGTCTC 1 cut(s) 194
Bsp1286I GDGCHC 2 cut(s) 184, 861
Bsp143I GATC 4 cut(s) 91, 135, 367, 931
Bsp19I CCATGG 1 cut(s) 361
BspACI CCGC 5 cut(s) 533, 686, 758, 788, 909
BspCNI CTCAG 1 cut(s) 459
BspMAI CTGCAG 3 cut(s) 10, 76, 433
BspMI ACCTGC 2 cut(s) 239, 735
BspOI GCTAGC 2 cut(s) 489, 608
BspPI GGATC 3 cut(s) 86, 375, 926
BspTNI GGTCTC 1 cut(s) 194
BsrBI CCGCTC 2 cut(s) 533, 909
BsrI ACTGG 3 cut(s) 164, 666, 830
BssECI CCNNGG 4 cut(s) 233, 361, 708, 982
BssMI GATC 4 cut(s) 91, 135, 367, 931
BssT1I CCWWGG 3 cut(s) 361, 708, 982
Bst2UI CCWGG 2 cut(s) 128, 234
BstAPI GCANNNNNTGC 1 cut(s) 644
BstC8I GCNNGC 3 cut(s) 487, 606, 649
BstDEI CTNAG 3 cut(s) 351, 467, 496
BstDSI CCRYGG 1 cut(s) 361
BstEII GGTNACC 1 cut(s) 977
BstF5I GGATG 2 cut(s) 304, 626
BstH2I RGCGCY 2 cut(s) 524, 576
BstHHI GCGC 2 cut(s) 523, 575
BstKTI GATC 4 cut(s) 94, 138, 370, 934
BstMAI GTCTC 2 cut(s) 194, 500
BstMBI GATC 4 cut(s) 91, 135, 367, 931
BstMWI GCNNNNNNNGC 4 cut(s) 518, 644, 692, 785
BstNI CCWGG 2 cut(s) 128, 234
BstNSI RCATGY 2 cut(s) 596, 617
BstPAI GACNNNNGTC 1 cut(s) 818
BstPI GGTNACC 1 cut(s) 977
BstSCI CCNGG 2 cut(s) 126, 232
BstSFI CTRYAG 5 cut(s) 6, 72, 429, 867, 999
BstV1I GCAGC 5 cut(s) 20, 136, 415, 469, 650
BstV2I GAAGAC 1 cut(s) 729
BstX2I RGATCY 1 cut(s) 367
BstYI RGATCY 1 cut(s) 367
BtgI CCRYGG 1 cut(s) 361
BtsCI GGATG 2 cut(s) 304, 626
BtsI GCAGTG 2 cut(s) 423, 786
BtsIMutI CAGTG 2 cut(s) 423, 786
BveI ACCTGC 2 cut(s) 239, 735
Cac8I GCNNGC 3 cut(s) 487, 606, 649
CaiI CAGNNNCTG 2 cut(s) 133, 644
CfoI GCGC 2 cut(s) 523, 575
Cfr13I GGNCC 3 cut(s) 124, 827, 941
CsiI ACCWGGT 1 cut(s) 126
Csp6I GTAC 2 cut(s) 421, 1018
CviAII CATG 6 cut(s) 347, 362, 442, 593, 614, 1029
CviQI GTAC 2 cut(s) 421, 1018
DdeI CTNAG 3 cut(s) 351, 467, 496
DpnI GATC 4 cut(s) 93, 137, 369, 933
DpnII GATC 4 cut(s) 91, 135, 367, 931
Eco130I CCWWGG 3 cut(s) 361, 708, 982
Eco24I GRGCYC 1 cut(s) 184
Eco31I GGTCTC 1 cut(s) 194
Eco47I GGWCC 3 cut(s) 124, 827, 941
Eco47III AGCGCT 1 cut(s) 522
Eco57I CTGAAG 1 cut(s) 911
Eco91I GGTNACC 1 cut(s) 977
EcoO65I GGTNACC 1 cut(s) 977
EcoRII CCWGG 2 cut(s) 126, 232
EcoT14I CCWWGG 3 cut(s) 361, 708, 982
EcoT38I GRGCYC 1 cut(s) 184
ErhI CCWWGG 3 cut(s) 361, 708, 982
FaeI CATG 6 cut(s) 350, 365, 445, 596, 617, 1032
FalI AAGNNNNNCTT 2 cut(s) 884, 916
FaqI GGGAC 1 cut(s) 1000
FatI CATG 6 cut(s) 346, 361, 441, 592, 613, 1028
FauI CCCGC 1 cut(s) 902
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 6 cut(s) 6, 9, 150, 429, 483, 639
FokI GGATG 2 cut(s) 311, 613
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 6 cut(s) 6, 9, 150, 429, 483, 639
FspBI CTAG 3 cut(s) 486, 605, 993
GlaI GCGC 2 cut(s) 522, 574
GluI GCNGC 6 cut(s) 6, 9, 150, 429, 483, 639
GsaI CCCAGC 2 cut(s) 407, 579
HaeII RGCGCY 2 cut(s) 524, 576
HapII CCGG 1 cut(s) 842
HhaI GCGC 2 cut(s) 523, 575
Hin1II CATG 6 cut(s) 350, 365, 445, 596, 617, 1032
Hin6I GCGC 2 cut(s) 521, 573
HinP1I GCGC 2 cut(s) 521, 573
HindIII AAGCTT 1 cut(s) 676
HinfI GANTC 2 cut(s) 334, 598
HpaII CCGG 1 cut(s) 842
HphI GGTGA 6 cut(s) 221, 271, 400, 802, 853, 928
Hpy166II GTNNAC 2 cut(s) 423, 1018
Hpy188I TCNGA 4 cut(s) 58, 84, 468, 851
Hpy188III TCNNGA 2 cut(s) 110, 993
Hpy8I GTNNAC 2 cut(s) 423, 1018
HpyAV CCTTC 8 cut(s) 171, 256, 500, 778, 787, 791, 799, 907
HpyCH4V TGCA 6 cut(s) 8, 24, 74, 280, 431, 647
HpyF10VI GCNNNNNNNGC 4 cut(s) 518, 644, 692, 785
HpyF3I CTNAG 3 cut(s) 351, 467, 496
Hsp92II CATG 6 cut(s) 350, 365, 445, 596, 617, 1032
HspAI GCGC 2 cut(s) 521, 573
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 4 cut(s) 91, 135, 367, 931
LmnI GCTCC 3 cut(s) 16, 518, 535
Lsp1109I GCAGC 5 cut(s) 20, 136, 415, 469, 650
LweI GCATC 2 cut(s) 635, 745
MabI ACCWGGT 1 cut(s) 126
MaeI CTAG 3 cut(s) 486, 605, 993
MaeIII GTNAC 3 cut(s) 811, 832, 977
MalI GATC 4 cut(s) 93, 137, 369, 933
MbiI CCGCTC 2 cut(s) 533, 909
MboI GATC 4 cut(s) 91, 135, 367, 931
MboII GAAGA 1 cut(s) 734
MflI RGATCY 1 cut(s) 367
MhlI GDGCHC 2 cut(s) 184, 861
MluCI AATT 4 cut(s) 86, 114, 228, 737
MmeI TCCRAC 1 cut(s) 565
MnlI CCTC 5 cut(s) 40, 115, 199, 563, 849
MseI TTAA 4 cut(s) 117, 159, 288, 671
MspI CCGG 1 cut(s) 842
MspR9I CCNGG 2 cut(s) 128, 234
MvaI CCWGG 2 cut(s) 128, 234
MwoI GCNNNNNNNGC 4 cut(s) 518, 644, 692, 785
NcoI CCATGG 1 cut(s) 361
NdeII GATC 4 cut(s) 91, 135, 367, 931
NheI GCTAGC 2 cut(s) 485, 604
NlaIII CATG 6 cut(s) 350, 365, 445, 596, 617, 1032
NmuCI GTSAC 1 cut(s) 811
NspI RCATGY 2 cut(s) 596, 617
PaqCI CACCTGC 1 cut(s) 239
PciI ACATGT 2 cut(s) 592, 613
PfeI GAWTC 2 cut(s) 334, 598
PkrI GCNGC 6 cut(s) 7, 10, 151, 430, 484, 640
PscI ACATGT 2 cut(s) 592, 613
PshAI GACNNNNGTC 1 cut(s) 818
Psp6I CCWGG 2 cut(s) 126, 232
PspEI GGTNACC 1 cut(s) 977
PspFI CCCAGC 2 cut(s) 403, 575
PspGI CCWGG 2 cut(s) 126, 232
PspPI GGNCC 3 cut(s) 124, 827, 941
PstI CTGCAG 3 cut(s) 10, 76, 433
PstNI CAGNNNCTG 2 cut(s) 133, 644
PsuI RGATCY 1 cut(s) 367
RsaI GTAC 2 cut(s) 422, 1019
RsaNI GTAC 2 cut(s) 421, 1018
SaqAI TTAA 4 cut(s) 117, 159, 288, 671
SatI GCNGC 6 cut(s) 6, 9, 150, 429, 483, 639
Sau3AI GATC 4 cut(s) 91, 135, 367, 931
Sau96I GGNCC 3 cut(s) 124, 827, 941
ScrFI CCNGG 2 cut(s) 128, 234
SduI GDGCHC 2 cut(s) 184, 861
SexAI ACCWGGT 1 cut(s) 126
SfaNI GCATC 2 cut(s) 635, 745
SfcI CTRYAG 5 cut(s) 6, 72, 429, 867, 999
SinI GGWCC 3 cut(s) 124, 827, 941
Sse9I AATT 4 cut(s) 86, 114, 228, 737
SsiI CCGC 5 cut(s) 533, 686, 758, 788, 909
SspMI CTAG 3 cut(s) 486, 605, 993
StyD4I CCNGG 2 cut(s) 126, 232
StyI CCWWGG 3 cut(s) 361, 708, 982
TaqI TCGA 1 cut(s) 720
TasI AATT 4 cut(s) 86, 114, 228, 737
TatI WGTACW 1 cut(s) 420
TfiI GAWTC 2 cut(s) 334, 598
Tru1I TTAA 4 cut(s) 117, 159, 288, 671
Tru9I TTAA 4 cut(s) 117, 159, 288, 671
TscAI CASTG 2 cut(s) 430, 786
TseFI GTSAC 1 cut(s) 811
TseI GCWGC 6 cut(s) 5, 8, 149, 428, 482, 638
Tsp45I GTSAC 1 cut(s) 811
TspDTI ATGAA 1 cut(s) 315
TspGWI ACGGA 1 cut(s) 544
TspRI CASTG 2 cut(s) 430, 786
VpaK11BI GGWCC 3 cut(s) 124, 827, 941
XapI RAATTY 2 cut(s) 86, 737
XbaI TCTAGA 1 cut(s) 992
XceI RCATGY 2 cut(s) 596, 617
XspI CTAG 3 cut(s) 486, 605, 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.