Rroxscaffold_2G00080610

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001188
Physical Location & Seq
Reverse (-)
3589373 .. 3591725
2353 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00080610.1

Sequence Viewer

Length: 945 bp
ATGGCTGCACCTCCAAGTGATTCCATACCCTCTGTAATGGGGGCTTGGGTCTATTCAGAGTATGGAAAAACTTCTGATGTTCTGAAGTTTGATCCGAGTGTGGCTGTTCCTGAAATTAAAGAGGATCAGGTGCTGATCAAGGTGGTTGCTGCCTCTCTTAACCCAGTTGATTTCAAAAGGGCTCTTGGTTACTTCAAGGACACTGACTCTCCCCTACCTACAGTTCCAGGGTATGATGTGGCTGGTGTGGTGGTGAAGGTAGGAAGCCAAGTAACGAAGTTTAAGGTGGGGGATGAAGTATATGGGGATCTCAATGAGACAGCATTGGTGAACCCAAAAAGGTTTGGGTCTTTGGCACAGTACACTGCTGCAGAAGAAAGAGTATTGGCTCACAAACCCAAAAACCTGAGCTTTATTGAAGCTGCTAGCCTTCCCTTAGCCATTGAGACTGCCTATGAAGGGCTTGAAAGTGCCGAACTTTCTGCTGGTAAATCCATCCTTGTTTTGGGAGGCGCTGGGGCTGGCAAAGCATGTTTTGGTGCTTCCAAAGTGGTAGCTACTGCAAGCACTAGGAAACTGGATTTGTTGAGAAGCTTGGGTGCTGATTTGCCTATCGATTACACCAAGGTGAACATCGAAGACCTGCCAGAGAAATTTGATGTAGTGTATGATGCAGTTGGGGAGACTGACAAGGCTGTAAAGGCGGTGAAAGAAGGCGGGAAGGTAGTAACAATAGTAGGTCCAGCAACGCCACCAGCTATCATATTTGTGCTTACCTCTAGAGGGTCTATCTTGGAGAAACTGAAGCCTTACTTGGAGGGTGGGAAGGTGAAGCCAGTGCTTGATCCCACAGGTCCATATCCCTTTTCTAAAGTTGTGGAAGCATTTGGTTACCTTGAGAGTTCCAGAGCTACAGGAAAGGTGGTTGTATATCCCATCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

314

Amino Acids

33.18

Weight (kDa)

5.5

Isoelectric Point (pI)

30.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 41 - 133 7.8e-17 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 178 - 250 1.2e-08 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 199 - 310 2.5e-18 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 651
AciI CCGC 2 cut(s) 704, 717
AclWI GGATC 4 cut(s) 86, 132, 315, 839
AcsI RAATTY 1 cut(s) 653
AcuI CTGAAG 2 cut(s) 104, 824
AfaI GTAC 1 cut(s) 362
AfiI CCNNNNNNNGG 3 cut(s) 459, 505, 783
AgsI TTSAA 4 cut(s) 175, 196, 419, 467
AjnI CCWGG 1 cut(s) 226
AjuI GAANNNNNNNTTGG 2 cut(s) 797, 829
AleI CACNNNNGTG 1 cut(s) 626
AluBI AGCT 6 cut(s) 411, 422, 557, 594, 758, 911
AluI AGCT 6 cut(s) 411, 422, 557, 594, 758, 911
Alw26I GTCTC 3 cut(s) 311, 440, 677
AlwI GGATC 4 cut(s) 86, 132, 315, 839
AlwNI CAGNNNCTG 1 cut(s) 133
ApeKI GCWGC 4 cut(s) 5, 149, 368, 422
ApoI RAATTY 1 cut(s) 653
Asp700I GAANNNNTTC 1 cut(s) 70
AspLEI GCGC 1 cut(s) 515
AspS9I GGNCC 2 cut(s) 740, 854
AsuHPI GGTGA 5 cut(s) 265, 340, 640, 718, 841
AsuNHI GCTAGC 1 cut(s) 425
AvaII GGWCC 2 cut(s) 740, 854
BanII GRGCYC 1 cut(s) 184
BbsI GAAGAC 1 cut(s) 645
BbvI GCAGC 3 cut(s) 136, 355, 409
BccI CCATC 2 cut(s) 503, 944
BcgI CGANNNNNNTGC 2 cut(s) 464, 498
BciT130I CCWGG 1 cut(s) 228
BclI TGATCA 1 cut(s) 135
BcoDI GTCTC 3 cut(s) 311, 440, 677
BfaI CTAG 3 cut(s) 426, 570, 780
BfmI CTRYAG 3 cut(s) 219, 369, 912
BfoI RGCGCY 1 cut(s) 516
BfuAI ACCTGC 1 cut(s) 651
BisI GCNGC 4 cut(s) 6, 150, 369, 423
BlsI GCNGC 4 cut(s) 7, 151, 370, 424
Bme1390I CCNGG 1 cut(s) 228
Bme18I GGWCC 2 cut(s) 740, 854
BmgT120I GGNCC 2 cut(s) 740, 854
BmrFI CCNGG 1 cut(s) 228
BmrI ACTGGG 1 cut(s) 158
BmsI GCATC 1 cut(s) 661
BmtI GCTAGC 1 cut(s) 429
BmuI ACTGGG 1 cut(s) 158
BpiI GAAGAC 1 cut(s) 645
Bpu10I CCTNAGC 2 cut(s) 407, 436
BpuEI CTTGAG 1 cut(s) 917
Bsa29I ATCGAT 1 cut(s) 615
BsaJI CCNNGG 2 cut(s) 227, 624
BsaXI ACNNNNNCTCC 2 cut(s) 193, 223
Bsc4I CCNNNNNNNGG 3 cut(s) 459, 505, 783
Bse1I ACTGG 3 cut(s) 164, 582, 836
BseBI CCWGG 1 cut(s) 228
BseCI ATCGAT 1 cut(s) 615
BseDI CCNNGG 2 cut(s) 227, 624
BseGI GGATG 3 cut(s) 298, 495, 936
BseLI CCNNNNNNNGG 3 cut(s) 459, 505, 783
BseMII CTCAG 1 cut(s) 398
BseNI ACTGG 3 cut(s) 164, 582, 836
BseXI GCAGC 3 cut(s) 136, 355, 409
BseYI CCCAGC 1 cut(s) 515
BshVI ATCGAT 1 cut(s) 615
BslI CCNNNNNNNGG 3 cut(s) 459, 505, 783
BsmAI GTCTC 3 cut(s) 311, 440, 677
Bsp1286I GDGCHC 1 cut(s) 184
Bsp143I GATC 5 cut(s) 91, 124, 135, 307, 844
BspACI CCGC 2 cut(s) 704, 717
BspCNI CTCAG 1 cut(s) 399
BspDI ATCGAT 1 cut(s) 615
BspMAI CTGCAG 1 cut(s) 373
BspMI ACCTGC 1 cut(s) 651
BspOI GCTAGC 1 cut(s) 429
BspPI GGATC 4 cut(s) 86, 132, 315, 839
BsrI ACTGG 3 cut(s) 164, 582, 836
BssECI CCNNGG 2 cut(s) 227, 624
BssMI GATC 5 cut(s) 91, 124, 135, 307, 844
BssT1I CCWWGG 1 cut(s) 624
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 2 cut(s) 223, 360
BstC8I GCNNGC 3 cut(s) 427, 523, 565
BstDEI CTNAG 2 cut(s) 407, 436
BstEII GGTNACC 1 cut(s) 890
BstF5I GGATG 3 cut(s) 298, 495, 936
BstH2I RGCGCY 1 cut(s) 516
BstHHI GCGC 1 cut(s) 515
BstKTI GATC 5 cut(s) 94, 127, 138, 310, 847
BstMAI GTCTC 3 cut(s) 311, 440, 677
BstMBI GATC 5 cut(s) 91, 124, 135, 307, 844
BstMWI GCNNNNNNNGC 2 cut(s) 527, 701
BstNI CCWGG 1 cut(s) 228
BstNSI RCATGY 1 cut(s) 534
BstPI GGTNACC 1 cut(s) 890
BstSCI CCNGG 1 cut(s) 226
BstSFI CTRYAG 3 cut(s) 219, 369, 912
BstV1I GCAGC 3 cut(s) 136, 355, 409
BstV2I GAAGAC 1 cut(s) 645
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
Bsu15I ATCGAT 1 cut(s) 615
BsuTUI ATCGAT 1 cut(s) 615
BtsCI GGATG 3 cut(s) 298, 495, 936
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 3 cut(s) 201, 363, 843
BveI ACCTGC 1 cut(s) 651
Cac8I GCNNGC 3 cut(s) 427, 523, 565
CaiI CAGNNNCTG 1 cut(s) 133
CfoI GCGC 1 cut(s) 515
Cfr13I GGNCC 2 cut(s) 740, 854
ClaI ATCGAT 1 cut(s) 615
Csp6I GTAC 1 cut(s) 361
CviAII CATG 2 cut(s) 531, 942
CviQI GTAC 1 cut(s) 361
DdeI CTNAG 2 cut(s) 407, 436
DpnI GATC 5 cut(s) 93, 126, 137, 309, 846
DpnII GATC 5 cut(s) 91, 124, 135, 307, 844
Eco130I CCWWGG 1 cut(s) 624
Eco24I GRGCYC 1 cut(s) 184
Eco47I GGWCC 2 cut(s) 740, 854
Eco57I CTGAAG 2 cut(s) 104, 824
Eco91I GGTNACC 1 cut(s) 890
EcoO65I GGTNACC 1 cut(s) 890
EcoRII CCWGG 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 624
EcoT38I GRGCYC 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 624
FaeI CATG 2 cut(s) 534, 945
FalI AAGNNNNNCTT 2 cut(s) 797, 829
FatI CATG 2 cut(s) 530, 941
FauI CCCGC 1 cut(s) 710
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 4 cut(s) 6, 150, 369, 423
FokI GGATG 3 cut(s) 305, 482, 923
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 4 cut(s) 6, 150, 369, 423
FspBI CTAG 3 cut(s) 426, 570, 780
GlaI GCGC 1 cut(s) 514
GluI GCNGC 4 cut(s) 6, 150, 369, 423
GsaI CCCAGC 1 cut(s) 519
HaeII RGCGCY 1 cut(s) 516
HhaI GCGC 1 cut(s) 515
Hin1II CATG 2 cut(s) 534, 945
Hin6I GCGC 1 cut(s) 513
HinP1I GCGC 1 cut(s) 513
HindIII AAGCTT 1 cut(s) 592
HinfI GANTC 2 cut(s) 20, 206
HphI GGTGA 5 cut(s) 265, 340, 640, 718, 841
Hpy166II GTNNAC 3 cut(s) 331, 363, 631
Hpy188I TCNGA 4 cut(s) 58, 76, 84, 96
Hpy188III TCNNGA 3 cut(s) 110, 780, 906
Hpy8I GTNNAC 3 cut(s) 331, 363, 631
HpyAV CCTTC 6 cut(s) 250, 440, 452, 707, 715, 820
HpyCH4III ACNGT 2 cut(s) 223, 360
HpyCH4V TGCA 4 cut(s) 8, 371, 563, 674
HpyF10VI GCNNNNNNNGC 2 cut(s) 527, 701
HpyF3I CTNAG 2 cut(s) 407, 436
Hsp92II CATG 2 cut(s) 534, 945
HspAI GCGC 1 cut(s) 513
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 5 cut(s) 91, 124, 135, 307, 844
Lsp1109I GCAGC 3 cut(s) 136, 355, 409
LweI GCATC 1 cut(s) 661
MaeI CTAG 3 cut(s) 426, 570, 780
MaeIII GTNAC 4 cut(s) 188, 271, 727, 890
MalI GATC 5 cut(s) 93, 126, 137, 309, 846
MboI GATC 5 cut(s) 91, 124, 135, 307, 844
MboII GAAGA 2 cut(s) 386, 650
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 1 cut(s) 184
MluCI AATT 2 cut(s) 114, 653
MlyI GAGTC 1 cut(s) 200
MnlI CCTC 8 cut(s) 21, 40, 115, 163, 503, 776, 787, 811
MroXI GAANNNNTTC 1 cut(s) 70
MseI TTAA 3 cut(s) 117, 159, 282
MslI CAYNNNNRTG 2 cut(s) 626, 767
MspR9I CCNGG 1 cut(s) 228
MvaI CCWGG 1 cut(s) 228
MwoI GCNNNNNNNGC 2 cut(s) 527, 701
NdeII GATC 5 cut(s) 91, 124, 135, 307, 844
NheI GCTAGC 1 cut(s) 425
NlaIII CATG 2 cut(s) 534, 945
NspI RCATGY 1 cut(s) 534
OliI CACNNNNGTG 1 cut(s) 626
PdmI GAANNNNTTC 1 cut(s) 70
PfeI GAWTC 1 cut(s) 20
PkrI GCNGC 4 cut(s) 7, 151, 370, 424
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
Psp6I CCWGG 1 cut(s) 226
PspEI GGTNACC 1 cut(s) 890
PspFI CCCAGC 1 cut(s) 515
PspGI CCWGG 1 cut(s) 226
PspPI GGNCC 2 cut(s) 740, 854
PstI CTGCAG 1 cut(s) 373
PstNI CAGNNNCTG 1 cut(s) 133
PsuI RGATCY 1 cut(s) 307
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
RseI CAYNNNNRTG 2 cut(s) 626, 767
SaqAI TTAA 3 cut(s) 117, 159, 282
SatI GCNGC 4 cut(s) 6, 150, 369, 423
Sau3AI GATC 5 cut(s) 91, 124, 135, 307, 844
Sau96I GGNCC 2 cut(s) 740, 854
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 1 cut(s) 228
SduI GDGCHC 1 cut(s) 184
SfaNI GCATC 1 cut(s) 661
SfcI CTRYAG 3 cut(s) 219, 369, 912
SinI GGWCC 2 cut(s) 740, 854
SmiMI CAYNNNNRTG 2 cut(s) 626, 767
SmlI CTYRAG 1 cut(s) 896
SmoI CTYRAG 1 cut(s) 896
Sse9I AATT 2 cut(s) 114, 653
SsiI CCGC 2 cut(s) 704, 717
SspMI CTAG 3 cut(s) 426, 570, 780
StyD4I CCNGG 1 cut(s) 226
StyI CCWWGG 1 cut(s) 624
TaaI ACNGT 2 cut(s) 223, 360
TaqI TCGA 2 cut(s) 615, 636
TasI AATT 2 cut(s) 114, 653
TatI WGTACW 1 cut(s) 360
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 3 cut(s) 117, 159, 282
Tru9I TTAA 3 cut(s) 117, 159, 282
TscAI CASTG 3 cut(s) 208, 370, 843
TseI GCWGC 4 cut(s) 5, 149, 368, 422
TspDTI ATGAA 2 cut(s) 309, 471
TspRI CASTG 3 cut(s) 208, 370, 843
VpaK11BI GGWCC 2 cut(s) 740, 854
XapI RAATTY 1 cut(s) 653
XbaI TCTAGA 1 cut(s) 779
XceI RCATGY 1 cut(s) 534
XcmI CCANNNNNNNNNTGG 1 cut(s) 502
XmnI GAANNNNTTC 1 cut(s) 70
XspI CTAG 3 cut(s) 426, 570, 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.