RLG00000022113

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001188
Physical Location & Seq
Forward (+)
81031199 .. 81034169
2971 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022113

Sequence Viewer

Length: 942 bp
ATGGCTGCAGCTCCAAAGTATGGAAAAACTGCAGATGTTCTGAAATTTGATCCAAGTGTGGCTGTTCCTGAAATTAAAGAGGACCAGGTGCTGATCAAGGTTGTCGCTGCTTCTCTTAACCCAATTGATTTCATGAGAGCTCTTGGTGTCTTCAAGGACTTAGAGGGTGACGCTCCCTTACCTACAATTCCAGGGTATGAAGTGGCTGGTGTGGTGGTGAAGGTAGGAAGCCAAGTGCAGAAGTTTACGGTGGGGGATGAAGTGTGTGCTTGTTTGAGTAATAGCATTGAGATTCCCCAATATCATGCTAAGAATAGCCATGGGGATCTCAATGAGAAAGCATTGGTGAAACCAAACAGGCTGGGGTCTTTGGCAGAGTACACCGCTGCAGGAGAAACCATGTTGGCTCACAAACCCAAAAACCTGAGCTTTATTGAAGCTGCTAGCCTTCCCTTAGCTATTGAGACTGCGTATGGAGCGCTTGAAAGAGCGGAGCTTTCGGCTGGTAAATCAGTCCTTGTTTTGGGAGGCGCTGGGGGTGTTGGAACACATGTGATTCAGCTAGCTAAGCGTGTTTTCGGGGCTTCCATAGTAGCAGCTACTGCAAGCACTAAAAAACTGGATTTGTTGAGAAGCTTGGGTGCGGATTTGGCTATTGATTACACCAAGGAGAACATTGAAGACCTGCCAGAGAAATTTGATGTAGTCTATGATGCAGTTGGGCAGACTGACAAGGCAGTGAAGGCGGTGAAGGAAGGTGGGAATGTAGTGACAATAGTAGGGTCTATCTTGGAGAAACTGAAGCCTTACTTGGAGAGCGGGAAGGTGAAGCCAGTGCTTGATCCCACAGGTCCATATCCCTTTTCTAAAGTTGTTGAAGCACTTGGTTACCTTGAGACTTCAAAAGCTACAGGAAAGGTGGTTGTGTACCCCATCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

314

Amino Acids

32.96

Weight (kDa)

5.91

Isoelectric Point (pI)

22.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 27 - 139 2.9e-17 Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C PF16912 153 - 252 1.7e-06 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 180 - 259 2.1e-15 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 213 - 262 5e-06 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 263 - 309 1.7e-08 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 693
AccB7I CCANNNNNTGG 1 cut(s) 20
AccBSI CCGCTC 2 cut(s) 491, 819
AciI CCGC 5 cut(s) 384, 491, 644, 746, 819
AclWI GGATC 3 cut(s) 44, 333, 836
AcsI RAATTY 2 cut(s) 44, 695
AcuI CTGAAG 1 cut(s) 821
AfaI GTAC 2 cut(s) 380, 929
AfeI AGCGCT 1 cut(s) 480
AfiI CCNNNNNNNGG 2 cut(s) 20, 523
AflIII ACRYGT 1 cut(s) 550
AgsI TTSAA 6 cut(s) 154, 437, 485, 680, 878, 903
AjnI CCWGG 2 cut(s) 84, 190
AjuI GAANNNNNNNTTGG 2 cut(s) 794, 826
Alw21I GWGCWC 1 cut(s) 142
Alw26I GTCTC 2 cut(s) 458, 890
AlwI GGATC 3 cut(s) 44, 333, 836
AlwNI CAGNNNCTG 2 cut(s) 91, 602
Aor51HI AGCGCT 1 cut(s) 480
ApeKI GCWGC 6 cut(s) 5, 8, 107, 386, 440, 596
ApoI RAATTY 2 cut(s) 44, 695
AspLEI GCGC 2 cut(s) 481, 533
AspS9I GGNCC 2 cut(s) 82, 851
AsuHPI GGTGA 5 cut(s) 179, 229, 358, 760, 838
AsuNHI GCTAGC 2 cut(s) 443, 562
AvaII GGWCC 2 cut(s) 82, 851
BanII GRGCYC 1 cut(s) 142
BbsI GAAGAC 2 cut(s) 142, 687
Bbv12I GWGCWC 1 cut(s) 142
BbvI GCAGC 5 cut(s) 20, 94, 373, 427, 608
BccI CCATC 1 cut(s) 941
BciT130I CCWGG 2 cut(s) 86, 192
BclI TGATCA 1 cut(s) 93
BcoDI GTCTC 2 cut(s) 458, 890
BfaI CTAG 2 cut(s) 444, 563
BfmI CTRYAG 4 cut(s) 6, 30, 387, 909
BfoI RGCGCY 2 cut(s) 482, 534
BfuAI ACCTGC 1 cut(s) 693
BisI GCNGC 6 cut(s) 6, 9, 108, 387, 441, 597
BlpI GCTNAGC 1 cut(s) 567
BlsI GCNGC 6 cut(s) 7, 10, 109, 388, 442, 598
Bme1390I CCNGG 2 cut(s) 86, 192
Bme18I GGWCC 2 cut(s) 82, 851
BmgT120I GGNCC 2 cut(s) 82, 851
BmrFI CCNGG 2 cut(s) 86, 192
BmsI GCATC 1 cut(s) 703
BmtI GCTAGC 2 cut(s) 447, 566
BpiI GAAGAC 2 cut(s) 142, 687
Bpu10I CCTNAGC 2 cut(s) 425, 454
Bpu1102I GCTNAGC 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 914
BsaJI CCNNGG 3 cut(s) 191, 319, 666
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 523
Bse1I ACTGG 2 cut(s) 624, 833
BseBI CCWGG 2 cut(s) 86, 192
BseDI CCNNGG 3 cut(s) 191, 319, 666
BseGI GGATG 2 cut(s) 262, 933
BseLI CCNNNNNNNGG 2 cut(s) 20, 523
BseMII CTCAG 1 cut(s) 416
BseNI ACTGG 2 cut(s) 624, 833
BseXI GCAGC 5 cut(s) 20, 94, 373, 427, 608
BseYI CCCAGC 2 cut(s) 361, 533
BsgI GTGCAG 1 cut(s) 257
BsiHKAI GWGCWC 1 cut(s) 142
BslI CCNNNNNNNGG 2 cut(s) 20, 523
BsmAI GTCTC 2 cut(s) 458, 890
Bsp1286I GDGCHC 1 cut(s) 142
Bsp143I GATC 4 cut(s) 49, 93, 325, 841
Bsp1720I GCTNAGC 1 cut(s) 567
Bsp19I CCATGG 1 cut(s) 319
BspACI CCGC 5 cut(s) 384, 491, 644, 746, 819
BspCNI CTCAG 1 cut(s) 417
BspHI TCATGA 1 cut(s) 132
BspMAI CTGCAG 3 cut(s) 10, 34, 391
BspMI ACCTGC 1 cut(s) 693
BspOI GCTAGC 2 cut(s) 447, 566
BspPI GGATC 3 cut(s) 44, 333, 836
BsrBI CCGCTC 2 cut(s) 491, 819
BsrI ACTGG 2 cut(s) 624, 833
BssECI CCNNGG 3 cut(s) 191, 319, 666
BssMI GATC 4 cut(s) 49, 93, 325, 841
BssT1I CCWWGG 2 cut(s) 319, 666
Bst2UI CCWGG 2 cut(s) 86, 192
Bst4CI ACNGT 1 cut(s) 250
BstAPI GCANNNNNTGC 1 cut(s) 602
BstC8I GCNNGC 3 cut(s) 445, 564, 607
BstDEI CTNAG 5 cut(s) 160, 309, 425, 454, 567
BstDSI CCRYGG 1 cut(s) 319
BstEII GGTNACC 1 cut(s) 887
BstF5I GGATG 2 cut(s) 262, 933
BstH2I RGCGCY 2 cut(s) 482, 534
BstHHI GCGC 2 cut(s) 481, 533
BstKTI GATC 4 cut(s) 52, 96, 328, 844
BstMAI GTCTC 2 cut(s) 458, 890
BstMBI GATC 4 cut(s) 49, 93, 325, 841
BstMWI GCNNNNNNNGC 5 cut(s) 476, 568, 602, 650, 743
BstNI CCWGG 2 cut(s) 86, 192
BstNSI RCATGY 1 cut(s) 554
BstPI GGTNACC 1 cut(s) 887
BstSCI CCNGG 2 cut(s) 84, 190
BstSFI CTRYAG 4 cut(s) 6, 30, 387, 909
BstV1I GCAGC 5 cut(s) 20, 94, 373, 427, 608
BstV2I GAAGAC 2 cut(s) 142, 687
BstX2I RGATCY 1 cut(s) 325
BstYI RGATCY 1 cut(s) 325
BtgI CCRYGG 1 cut(s) 319
BtsCI GGATG 2 cut(s) 262, 933
BtsI GCAGTG 1 cut(s) 744
BtsIMutI CAGTG 2 cut(s) 744, 840
BveI ACCTGC 1 cut(s) 693
Cac8I GCNNGC 3 cut(s) 445, 564, 607
CaiI CAGNNNCTG 2 cut(s) 91, 602
CciI TCATGA 1 cut(s) 132
CfoI GCGC 2 cut(s) 481, 533
Cfr13I GGNCC 2 cut(s) 82, 851
CseI GACGC 1 cut(s) 179
CsiI ACCWGGT 1 cut(s) 84
Csp6I GTAC 2 cut(s) 379, 928
CviAII CATG 6 cut(s) 133, 305, 320, 400, 551, 939
CviQI GTAC 2 cut(s) 379, 928
DdeI CTNAG 5 cut(s) 160, 309, 425, 454, 567
DpnI GATC 4 cut(s) 51, 95, 327, 843
DpnII GATC 4 cut(s) 49, 93, 325, 841
Ecl136II GAGCTC 1 cut(s) 140
Eco130I CCWWGG 2 cut(s) 319, 666
Eco24I GRGCYC 1 cut(s) 142
Eco47I GGWCC 2 cut(s) 82, 851
Eco47III AGCGCT 1 cut(s) 480
Eco53kI GAGCTC 1 cut(s) 140
Eco57I CTGAAG 1 cut(s) 821
Eco91I GGTNACC 1 cut(s) 887
EcoICRI GAGCTC 1 cut(s) 140
EcoO65I GGTNACC 1 cut(s) 887
EcoRII CCWGG 2 cut(s) 84, 190
EcoT14I CCWWGG 2 cut(s) 319, 666
EcoT38I GRGCYC 1 cut(s) 142
ErhI CCWWGG 2 cut(s) 319, 666
FaeI CATG 6 cut(s) 136, 308, 323, 403, 554, 942
FalI AAGNNNNNCTT 2 cut(s) 794, 826
FatI CATG 6 cut(s) 132, 304, 319, 399, 550, 938
FauI CCCGC 1 cut(s) 812
FbaI TGATCA 1 cut(s) 93
Fnu4HI GCNGC 6 cut(s) 6, 9, 108, 387, 441, 597
FokI GGATG 2 cut(s) 269, 920
FriOI GRGCYC 1 cut(s) 142
Fsp4HI GCNGC 6 cut(s) 6, 9, 108, 387, 441, 597
FspBI CTAG 2 cut(s) 444, 563
GlaI GCGC 2 cut(s) 480, 532
GluI GCNGC 6 cut(s) 6, 9, 108, 387, 441, 597
GsaI CCCAGC 2 cut(s) 365, 537
HaeII RGCGCY 2 cut(s) 482, 534
HgaI GACGC 1 cut(s) 179
HhaI GCGC 2 cut(s) 481, 533
Hin1II CATG 6 cut(s) 136, 308, 323, 403, 554, 942
Hin6I GCGC 2 cut(s) 479, 531
HinP1I GCGC 2 cut(s) 479, 531
HindIII AAGCTT 1 cut(s) 634
HinfI GANTC 2 cut(s) 292, 556
HphI GGTGA 5 cut(s) 179, 229, 358, 760, 838
Hpy166II GTNNAC 3 cut(s) 246, 381, 928
Hpy188I TCNGA 1 cut(s) 42
Hpy188III TCNNGA 2 cut(s) 68, 133
Hpy8I GTNNAC 3 cut(s) 246, 381, 928
HpyAV CCTTC 6 cut(s) 214, 458, 736, 745, 749, 817
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4V TGCA 6 cut(s) 8, 32, 238, 389, 605, 716
HpyF10VI GCNNNNNNNGC 5 cut(s) 476, 568, 602, 650, 743
HpyF3I CTNAG 5 cut(s) 160, 309, 425, 454, 567
Hsp92II CATG 6 cut(s) 136, 308, 323, 403, 554, 942
HspAI GCGC 2 cut(s) 479, 531
Ksp22I TGATCA 1 cut(s) 93
Kzo9I GATC 4 cut(s) 49, 93, 325, 841
LmnI GCTCC 4 cut(s) 16, 178, 476, 493
Lsp1109I GCAGC 5 cut(s) 20, 94, 373, 427, 608
LweI GCATC 1 cut(s) 703
MabI ACCWGGT 1 cut(s) 84
MaeI CTAG 2 cut(s) 444, 563
MaeIII GTNAC 3 cut(s) 167, 769, 887
MalI GATC 4 cut(s) 51, 95, 327, 843
MbiI CCGCTC 2 cut(s) 491, 819
MboI GATC 4 cut(s) 49, 93, 325, 841
MboII GAAGA 2 cut(s) 142, 692
MfeI CAATTG 1 cut(s) 123
MflI RGATCY 1 cut(s) 325
MhlI GDGCHC 1 cut(s) 142
MluCI AATT 5 cut(s) 44, 72, 123, 186, 695
MmeI TCCRAC 1 cut(s) 523
MnlI CCTC 3 cut(s) 73, 157, 521
MseI TTAA 2 cut(s) 75, 117
MspA1I CMGCKG 1 cut(s) 386
MspR9I CCNGG 2 cut(s) 86, 192
MunI CAATTG 1 cut(s) 123
MvaI CCWGG 2 cut(s) 86, 192
MwoI GCNNNNNNNGC 5 cut(s) 476, 568, 602, 650, 743
NcoI CCATGG 1 cut(s) 319
NdeII GATC 4 cut(s) 49, 93, 325, 841
NheI GCTAGC 2 cut(s) 443, 562
NlaIII CATG 6 cut(s) 136, 308, 323, 403, 554, 942
NmuCI GTSAC 2 cut(s) 167, 769
NspI RCATGY 1 cut(s) 554
PagI TCATGA 1 cut(s) 132
PciI ACATGT 1 cut(s) 550
PfeI GAWTC 2 cut(s) 292, 556
PflMI CCANNNNNTGG 1 cut(s) 20
PkrI GCNGC 6 cut(s) 7, 10, 109, 388, 442, 598
PscI ACATGT 1 cut(s) 550
Psp124BI GAGCTC 1 cut(s) 142
Psp6I CCWGG 2 cut(s) 84, 190
PspEI GGTNACC 1 cut(s) 887
PspFI CCCAGC 2 cut(s) 361, 533
PspGI CCWGG 2 cut(s) 84, 190
PspPI GGNCC 2 cut(s) 82, 851
PstI CTGCAG 3 cut(s) 10, 34, 391
PstNI CAGNNNCTG 2 cut(s) 91, 602
PsuI RGATCY 1 cut(s) 325
RsaI GTAC 2 cut(s) 380, 929
RsaNI GTAC 2 cut(s) 379, 928
SacI GAGCTC 1 cut(s) 142
SaqAI TTAA 2 cut(s) 75, 117
SatI GCNGC 6 cut(s) 6, 9, 108, 387, 441, 597
Sau3AI GATC 4 cut(s) 49, 93, 325, 841
Sau96I GGNCC 2 cut(s) 82, 851
ScrFI CCNGG 2 cut(s) 86, 192
SduI GDGCHC 1 cut(s) 142
SexAI ACCWGGT 1 cut(s) 84
SfaNI GCATC 1 cut(s) 703
SfcI CTRYAG 4 cut(s) 6, 30, 387, 909
SinI GGWCC 2 cut(s) 82, 851
SmlI CTYRAG 1 cut(s) 893
SmoI CTYRAG 1 cut(s) 893
Sse9I AATT 5 cut(s) 44, 72, 123, 186, 695
SsiI CCGC 5 cut(s) 384, 491, 644, 746, 819
SspMI CTAG 2 cut(s) 444, 563
SstI GAGCTC 1 cut(s) 142
StyD4I CCNGG 2 cut(s) 84, 190
StyI CCWWGG 2 cut(s) 319, 666
TaaI ACNGT 1 cut(s) 250
TasI AATT 5 cut(s) 44, 72, 123, 186, 695
TatI WGTACW 1 cut(s) 378
TfiI GAWTC 2 cut(s) 292, 556
Tru1I TTAA 2 cut(s) 75, 117
Tru9I TTAA 2 cut(s) 75, 117
TscAI CASTG 2 cut(s) 744, 840
TseFI GTSAC 2 cut(s) 167, 769
TseI GCWGC 6 cut(s) 5, 8, 107, 386, 440, 596
Tsp45I GTSAC 2 cut(s) 167, 769
TspDTI ATGAA 3 cut(s) 121, 213, 273
TspRI CASTG 2 cut(s) 744, 840
Van91I CCANNNNNTGG 1 cut(s) 20
VpaK11BI GGWCC 2 cut(s) 82, 851
XapI RAATTY 2 cut(s) 44, 695
XceI RCATGY 1 cut(s) 554
XspI CTAG 2 cut(s) 444, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.