MD09G1033200.v1.1

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001188
Physical Location & Seq
Reverse (-)
2040090 .. 2041792
1703 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1033200.v1.1.491

Sequence Viewer

Length: 978 bp
ATGGCGGCTGTTGTAGCTTCAAGTGATTCAGTTTCCATACCATCAGTAAACAAGGCGTGGGTGTACTCAGAGTACGGAAAGTCTGCAGATGTTTTGAAGTTCGATCCGAATGTTCCTGTTCCTGAAATAAAGGAAGACCAGGTGCTGATCAAGGTGGTTGCTGCATCTCTCAACCCAGTTGATTTCAAAAGGATGCTTGGCTACTTCAAGGACTCTGACTCTTCACCCCCTACGGTTCCAGGGTATGATGTTGCTGGTGTGGTGGTGAAAGTAGGAAGCCGGGTGACAAAGTTTAATGTGGGGGATGAAGTATATGGGGATCTCAACGAGAAGGCTATCGATAAGCCGAAAAAGATCGGATCTTTGGCCGAGTACACTGCTGCAGAAGAAAGAGTGTTAGCTCTTAAGCCCAAAAATCTGAGTTTTGTTGAAGCTGCTAGCCTTCCCCTGGCCATTGAGACTGCCTATGAAGGGCTCGAACGAGTTGAATTCTCTGCCGGTAAATCCATCCTTGTTTTGGGAGGCGCTGGGGGAGTCGGAACACATGTTATTCAGCTAGCAAAGCATGTGTTTGGTGCTTCAAAAGTAGCAGCTACAGCGAGCACGAAAAAACTTGATCTTTTGAGAAGCTTTGGTGCTGATTTGGCTATTGATTACACCAAGGAGAACTTCGAAGACCTGCCCGAGAAATTCGATGTGGTTTATGATGCAGTTGGGGAGACTGACAGGGCATTGAAGGCGGTGAAGGAAGGCGGGAAGGTATTGACAATTGTTCCACCTGCGCCACCGGCATTCCTTTTTATGCTGACTTCAACCGGAACTGTATTAGAGAAACTGAAGCCTTACTTGGAGAGTGGGAAGGTGAGGCCGGTGCTTGATCCCACCGGCCCGTATCCATTTTCGAAGACCGTTGAAGCATTTGCCTATCTTGAAACCTCCAGAGCTACCGGAAAGGTGGTTGTGTATCCCATTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

326

Amino Acids

34.75

Weight (kDa)

5.67

Isoelectric Point (pI)

24.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 45 - 137 3.1e-16 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 178 - 257 4e-13 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 211 - 321 4.5e-20 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 787
Acc36I ACCTGC 2 cut(s) 687, 787
AciI CCGC 3 cut(s) 5, 740, 753
AclWI GGATC 4 cut(s) 98, 327, 367, 872
AcoI YGGCCR 2 cut(s) 366, 450
AcsI RAATTY 2 cut(s) 488, 689
AcuI CTGAAG 1 cut(s) 857
AfaI GTAC 3 cut(s) 65, 74, 374
AfiI CCNNNNNNNGG 3 cut(s) 448, 471, 517
AflII CTTAAG 1 cut(s) 404
AflIII ACRYGT 1 cut(s) 544
AjnI CCWGG 3 cut(s) 138, 238, 447
AjuI GAANNNNNNNTTGG 4 cut(s) 653, 685, 830, 862
AluBI AGCT 7 cut(s) 17, 401, 434, 556, 593, 630, 944
AluI AGCT 7 cut(s) 17, 401, 434, 556, 593, 630, 944
Alw21I GWGCWC 1 cut(s) 605
Alw26I GTCTC 2 cut(s) 452, 713
AlwI GGATC 4 cut(s) 98, 327, 367, 872
AlwNI CAGNNNCTG 1 cut(s) 145
Ama87I CYCGRG 1 cut(s) 683
AoxI GGCC 4 cut(s) 366, 450, 866, 886
ApeKI GCWGC 4 cut(s) 161, 380, 434, 590
ApoI RAATTY 2 cut(s) 488, 689
AspLEI GCGC 2 cut(s) 527, 784
AspS9I GGNCC 1 cut(s) 887
AsuC2I CCSGG 1 cut(s) 281
AsuHPI GGTGA 5 cut(s) 216, 277, 295, 754, 874
AsuII TTCGAA 2 cut(s) 672, 902
AsuNHI GCTAGC 2 cut(s) 437, 556
AvaI CYCGRG 1 cut(s) 683
BalI TGGCCA 1 cut(s) 452
BanII GRGCYC 1 cut(s) 477
BbsI GAAGAC 3 cut(s) 141, 681, 911
Bbv12I GWGCWC 1 cut(s) 605
BbvI GCAGC 4 cut(s) 148, 367, 421, 602
BccI CCATC 2 cut(s) 49, 515
BcgI CGANNNNNNTGC 2 cut(s) 359, 393
BciT130I CCWGG 3 cut(s) 140, 240, 449
BciVI GTATCC 2 cut(s) 903, 975
BclI TGATCA 1 cut(s) 147
BcnI CCSGG 1 cut(s) 281
BcoDI GTCTC 2 cut(s) 452, 713
BfaI CTAG 2 cut(s) 438, 557
BfmI CTRYAG 3 cut(s) 84, 381, 594
BfoI RGCGCY 1 cut(s) 528
BfrI CTTAAG 1 cut(s) 404
BfuAI ACCTGC 2 cut(s) 687, 787
BfuI GTATCC 2 cut(s) 903, 975
BisI GCNGC 5 cut(s) 6, 162, 381, 435, 591
BlsI GCNGC 5 cut(s) 7, 163, 382, 436, 592
Bme1390I CCNGG 4 cut(s) 140, 240, 281, 449
BmeT110I CYCGRG 1 cut(s) 683
BmgT120I GGNCC 1 cut(s) 887
BmiI GGNNCC 1 cut(s) 237
BmrFI CCNGG 4 cut(s) 140, 240, 281, 449
BmrI ACTGGG 1 cut(s) 170
BmsI GCATC 3 cut(s) 173, 183, 697
BmtI GCTAGC 2 cut(s) 441, 560
BmuI ACTGGG 1 cut(s) 170
BpiI GAAGAC 3 cut(s) 141, 681, 911
BpmI CTGGAG 1 cut(s) 922
Bpu14I TTCGAA 2 cut(s) 672, 902
BpuMI CCSGG 1 cut(s) 281
Bsa29I ATCGAT 1 cut(s) 339
BsaJI CCNNGG 3 cut(s) 239, 447, 660
BsaWI WCCGGW 2 cut(s) 815, 947
Bsc4I CCNNNNNNNGG 3 cut(s) 448, 471, 517
Bse118I RCCGGY 4 cut(s) 497, 787, 868, 884
Bse1I ACTGG 1 cut(s) 176
BseBI CCWGG 3 cut(s) 140, 240, 449
BseCI ATCGAT 1 cut(s) 339
BseDI CCNNGG 3 cut(s) 239, 447, 660
BseGI GGATG 3 cut(s) 198, 310, 507
BseLI CCNNNNNNNGG 3 cut(s) 448, 471, 517
BseMII CTCAG 2 cut(s) 81, 410
BseNI ACTGG 1 cut(s) 176
BseXI GCAGC 4 cut(s) 148, 367, 421, 602
BseYI CCCAGC 1 cut(s) 527
BshFI GGCC 4 cut(s) 368, 452, 868, 888
BshVI ATCGAT 1 cut(s) 339
BsiHKAI GWGCWC 1 cut(s) 605
BsiHKCI CYCGRG 1 cut(s) 683
BsiSI CCGG 7 cut(s) 280, 498, 788, 816, 869, 885, 948
BslI CCNNNNNNNGG 3 cut(s) 448, 471, 517
BsmAI GTCTC 2 cut(s) 452, 713
BsmI GAATGC 1 cut(s) 791
BsnI GGCC 4 cut(s) 368, 452, 868, 888
BsoBI CYCGRG 1 cut(s) 683
Bsp119I TTCGAA 2 cut(s) 672, 902
Bsp1286I GDGCHC 2 cut(s) 477, 605
Bsp143I GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 877
BspACI CCGC 3 cut(s) 5, 740, 753
BspANI GGCC 4 cut(s) 368, 452, 868, 888
BspCNI CTCAG 2 cut(s) 80, 411
BspDI ATCGAT 1 cut(s) 339
BspLI GGNNCC 1 cut(s) 237
BspMAI CTGCAG 2 cut(s) 88, 385
BspMI ACCTGC 2 cut(s) 687, 787
BspOI GCTAGC 2 cut(s) 441, 560
BspPI GGATC 4 cut(s) 98, 327, 367, 872
BspT104I TTCGAA 2 cut(s) 672, 902
BspTI CTTAAG 1 cut(s) 404
BsrFI RCCGGY 4 cut(s) 497, 787, 868, 884
BsrI ACTGG 1 cut(s) 176
BssAI RCCGGY 4 cut(s) 497, 787, 868, 884
BssECI CCNNGG 3 cut(s) 239, 447, 660
BssMI GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 877
BssT1I CCWWGG 1 cut(s) 660
Bst2UI CCWGG 3 cut(s) 140, 240, 449
Bst4CI ACNGT 3 cut(s) 235, 823, 910
Bst6I CTCTTC 1 cut(s) 226
BstAFI CTTAAG 1 cut(s) 404
BstBI TTCGAA 2 cut(s) 672, 902
BstC8I GCNNGC 3 cut(s) 439, 558, 601
BstDEI CTNAG 2 cut(s) 67, 419
BstF5I GGATG 3 cut(s) 198, 310, 507
BstH2I RGCGCY 1 cut(s) 528
BstHHI GCGC 2 cut(s) 527, 784
BstKTI GATC 7 cut(s) 106, 150, 322, 357, 362, 619, 880
BstMAI GTCTC 2 cut(s) 452, 713
BstMBI GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 877
BstMWI GCNNNNNNNGC 6 cut(s) 14, 562, 596, 644, 737, 788
BstNI CCWGG 3 cut(s) 140, 240, 449
BstNSI RCATGY 2 cut(s) 548, 569
BstSCI CCNGG 4 cut(s) 138, 238, 279, 447
BstSFI CTRYAG 3 cut(s) 84, 381, 594
BstV1I GCAGC 4 cut(s) 148, 367, 421, 602
BstV2I GAAGAC 3 cut(s) 141, 681, 911
BstX2I RGATCY 2 cut(s) 319, 359
BstYI RGATCY 2 cut(s) 319, 359
Bsu15I ATCGAT 1 cut(s) 339
BsuI GTATCC 2 cut(s) 903, 975
BsuRI GGCC 4 cut(s) 368, 452, 868, 888
BsuTUI ATCGAT 1 cut(s) 339
BtsCI GGATG 3 cut(s) 198, 310, 507
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 1 cut(s) 375
BveI ACCTGC 2 cut(s) 687, 787
Cac8I GCNNGC 3 cut(s) 439, 558, 601
CaiI CAGNNNCTG 1 cut(s) 145
CfoI GCGC 2 cut(s) 527, 784
Cfr10I RCCGGY 4 cut(s) 497, 787, 868, 884
Cfr13I GGNCC 1 cut(s) 887
ClaI ATCGAT 1 cut(s) 339
CsiI ACCWGGT 1 cut(s) 138
Csp6I GTAC 3 cut(s) 64, 73, 373
CviAII CATG 3 cut(s) 545, 566, 975
CviQI GTAC 3 cut(s) 64, 73, 373
DdeI CTNAG 2 cut(s) 67, 419
DpnI GATC 7 cut(s) 105, 149, 321, 356, 361, 618, 879
DpnII GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 877
EaeI YGGCCR 2 cut(s) 366, 450
Eam1104I CTCTTC 1 cut(s) 226
EarI CTCTTC 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 660
Eco24I GRGCYC 1 cut(s) 477
Eco57I CTGAAG 1 cut(s) 857
Eco88I CYCGRG 1 cut(s) 683
EcoRI GAATTC 1 cut(s) 488
EcoRII CCWGG 3 cut(s) 138, 238, 447
EcoT14I CCWWGG 1 cut(s) 660
EcoT38I GRGCYC 1 cut(s) 477
ErhI CCWWGG 1 cut(s) 660
FaeI CATG 3 cut(s) 548, 569, 978
FalI AAGNNNNNCTT 4 cut(s) 653, 685, 830, 862
FatI CATG 3 cut(s) 544, 565, 974
FauI CCCGC 1 cut(s) 746
FbaI TGATCA 1 cut(s) 147
Fnu4HI GCNGC 5 cut(s) 6, 162, 381, 435, 591
FokI GGATG 3 cut(s) 205, 317, 494
FriOI GRGCYC 1 cut(s) 477
Fsp4HI GCNGC 5 cut(s) 6, 162, 381, 435, 591
FspBI CTAG 2 cut(s) 438, 557
GlaI GCGC 2 cut(s) 526, 783
GluI GCNGC 5 cut(s) 6, 162, 381, 435, 591
GsaI CCCAGC 1 cut(s) 531
GsuI CTGGAG 1 cut(s) 922
HaeII RGCGCY 1 cut(s) 528
HaeIII GGCC 4 cut(s) 368, 452, 868, 888
HapII CCGG 7 cut(s) 280, 498, 788, 816, 869, 885, 948
HhaI GCGC 2 cut(s) 527, 784
Hin1II CATG 3 cut(s) 548, 569, 978
Hin6I GCGC 2 cut(s) 525, 782
HinP1I GCGC 2 cut(s) 525, 782
HindIII AAGCTT 1 cut(s) 628
HinfI GANTC 4 cut(s) 26, 212, 218, 534
HpaII CCGG 7 cut(s) 280, 498, 788, 816, 869, 885, 948
HphI GGTGA 5 cut(s) 216, 277, 295, 754, 874
Hpy166II GTNNAC 3 cut(s) 49, 64, 375
Hpy188I TCNGA 6 cut(s) 70, 108, 217, 359, 420, 539
Hpy188III TCNNGA 3 cut(s) 122, 929, 939
Hpy8I GTNNAC 3 cut(s) 49, 64, 375
HpyAV CCTTC 8 cut(s) 325, 452, 464, 730, 739, 743, 751, 853
HpyCH4III ACNGT 3 cut(s) 235, 823, 910
HpyCH4V TGCA 4 cut(s) 86, 164, 383, 710
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 562, 596, 644, 737, 788
HpyF3I CTNAG 2 cut(s) 67, 419
Hsp92II CATG 3 cut(s) 548, 569, 978
HspAI GCGC 2 cut(s) 525, 782
Ksp22I TGATCA 1 cut(s) 147
Kzo9I GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 877
Lsp1109I GCAGC 4 cut(s) 148, 367, 421, 602
LweI GCATC 3 cut(s) 173, 183, 697
MabI ACCWGGT 1 cut(s) 138
MaeI CTAG 2 cut(s) 438, 557
MaeIII GTNAC 1 cut(s) 283
MalI GATC 7 cut(s) 105, 149, 321, 356, 361, 618, 879
MboI GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 877
MboII GAAGA 5 cut(s) 146, 213, 398, 686, 916
MfeI CAATTG 1 cut(s) 768
MflI RGATCY 2 cut(s) 319, 359
MhlI GDGCHC 2 cut(s) 477, 605
MlsI TGGCCA 1 cut(s) 452
MluCI AATT 3 cut(s) 488, 689, 768
MluNI TGGCCA 1 cut(s) 452
MlyI GAGTC 3 cut(s) 206, 212, 543
MmeI TCCRAC 1 cut(s) 517
MnlI CCTC 3 cut(s) 515, 858, 946
Mox20I TGGCCA 1 cut(s) 452
MscI TGGCCA 1 cut(s) 452
MseI TTAA 2 cut(s) 294, 405
Msp20I TGGCCA 1 cut(s) 452
MspCI CTTAAG 1 cut(s) 404
MspI CCGG 7 cut(s) 280, 498, 788, 816, 869, 885, 948
MspR9I CCNGG 4 cut(s) 140, 240, 281, 449
MunI CAATTG 1 cut(s) 768
Mva1269I GAATGC 1 cut(s) 791
MvaI CCWGG 3 cut(s) 140, 240, 449
MwoI GCNNNNNNNGC 6 cut(s) 14, 562, 596, 644, 737, 788
NciI CCSGG 1 cut(s) 281
NdeII GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 877
NheI GCTAGC 2 cut(s) 437, 556
NlaIII CATG 3 cut(s) 548, 569, 978
NlaIV GGNNCC 1 cut(s) 237
NmeAIII GCCGAG 1 cut(s) 394
NmuCI GTSAC 1 cut(s) 283
NspI RCATGY 2 cut(s) 548, 569
NspV TTCGAA 2 cut(s) 672, 902
PaqCI CACCTGC 1 cut(s) 787
PciI ACATGT 1 cut(s) 544
PctI GAATGC 1 cut(s) 791
PfeI GAWTC 1 cut(s) 26
PkrI GCNGC 5 cut(s) 7, 163, 382, 436, 592
PleI GAGTC 3 cut(s) 206, 212, 542
PpsI GAGTC 3 cut(s) 206, 212, 542
PscI ACATGT 1 cut(s) 544
Psp6I CCWGG 3 cut(s) 138, 238, 447
PspFI CCCAGC 1 cut(s) 527
PspGI CCWGG 3 cut(s) 138, 238, 447
PspN4I GGNNCC 1 cut(s) 237
PspPI GGNCC 1 cut(s) 887
PstI CTGCAG 2 cut(s) 88, 385
PstNI CAGNNNCTG 1 cut(s) 145
PsuI RGATCY 2 cut(s) 319, 359
RsaI GTAC 3 cut(s) 65, 74, 374
RsaNI GTAC 3 cut(s) 64, 73, 373
SaqAI TTAA 2 cut(s) 294, 405
SatI GCNGC 5 cut(s) 6, 162, 381, 435, 591
Sau3AI GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 877
Sau96I GGNCC 1 cut(s) 887
SchI GAGTC 3 cut(s) 206, 212, 543
ScrFI CCNGG 4 cut(s) 140, 240, 281, 449
SduI GDGCHC 2 cut(s) 477, 605
SexAI ACCWGGT 1 cut(s) 138
SfaNI GCATC 3 cut(s) 173, 183, 697
SfcI CTRYAG 3 cut(s) 84, 381, 594
SfuI TTCGAA 2 cut(s) 672, 902
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
Sse9I AATT 3 cut(s) 488, 689, 768
SsiI CCGC 3 cut(s) 5, 740, 753
SspMI CTAG 2 cut(s) 438, 557
StyD4I CCNGG 4 cut(s) 138, 238, 279, 447
StyI CCWWGG 1 cut(s) 660
TaaI ACNGT 3 cut(s) 235, 823, 910
TaqI TCGA 6 cut(s) 102, 339, 477, 672, 693, 902
TasI AATT 3 cut(s) 488, 689, 768
TatI WGTACW 2 cut(s) 63, 372
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 26
Tru1I TTAA 2 cut(s) 294, 405
Tru9I TTAA 2 cut(s) 294, 405
TscAI CASTG 1 cut(s) 382
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 4 cut(s) 161, 380, 434, 590
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 2 cut(s) 321, 483
TspGWI ACGGA 1 cut(s) 90
TspRI CASTG 1 cut(s) 382
Vha464I CTTAAG 1 cut(s) 404
XapI RAATTY 2 cut(s) 488, 689
XceI RCATGY 2 cut(s) 548, 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XspI CTAG 2 cut(s) 438, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.