pycom111g02700

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0001188
Physical Location & Seq
Reverse (-)
1622795 .. 1624846
2052 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g02700.4

Sequence Viewer

Length: 981 bp
ATGGCGGCTGCTGTAGCTTCAAGTGATTCAGTTTCCATACCATCTGTAAACAAGGCGTGGGTGTACCCAGAGTACGGAAAGTCTGCAGATGTTTTGAAGTTCGATCCGAATGTTCCTGTTCCTGAAATAAAGGAAGACCAGGTGCTGATCAAGGTGGTTGCTGCATCTCTCAACCCAGTCGATTTCAAAAGGATGCTTGGCTACTTCAAGGACTATGACTCTTCACCCCCTACAGTTCCAGGGTATGATGTTGCTGGTGTGGTGGTGAAAGTAGGAAGCCAGGTGACAAAGTTTAAGGTGGGGGATGAAGTATATGGGGATCTCAACGAGAAGCCTCTCACTAAGCCGAAAAGGATCGGATCTTTGGCCGAGTACACTGCTGCAGAAGAAAGAGTGTTGGCTCTCAAGCCAAAAAATCTGAGTTTTGTTGAAGCTGCTAGCCTTCCCCTGGCCATTGAGACTGCCTATGAAGGGCTCGAACGAGTTGAATTCTCTGCTGGTAAATCCATCCTTGTTTTGGGAGGCGCTGGGGGAGTCGGAACACATGTTATTCAGCTAGCAAAGCATGTGTTTGGTGCTTCAAAAGTAGCAGCTACAGCGAGCACGAAAAAACTTGATCTTTTGAGAAGCTTGGGTGCTGATTTGGCTATTGATTACACCAAGGAGAACTTCGAAGAACCGCGCGAGAAATTTGATGTGGTTTATAATGCAGTTGGGGAGACTGACAGGGCAGTGAAGGCGGTGAAGGAAGGCGGGAAGGTATTGACAATTGTACGTCCAGTAGCGCCACCGGCAATCCTATTTTTGCTGACTTCAACCGGAACTGTATTAGAGAAACTGAAGCCTTACTTGGAGAGTGGGAAGGTGAAGCCGGTGCTTGATCCCACTGGCCCGTATCCATTTTCGAAGACTGTTGAAGCATTTGCCTATCTTGAAACCTCCAGAGCTACCGGAAAGGTGGTTGTGTATCCCATCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

327

Amino Acids

34.92

Weight (kDa)

7.7

Isoelectric Point (pI)

23.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 705
AccII CGCG 2 cut(s) 682, 684
AciI CCGC 4 cut(s) 5, 680, 740, 753
AclWI GGATC 5 cut(s) 98, 327, 362, 367, 875
AcoI YGGCCR 2 cut(s) 366, 450
AcsI RAATTY 2 cut(s) 488, 689
AcuI CTGAAG 1 cut(s) 860
AfaI GTAC 4 cut(s) 65, 74, 374, 774
AfiI CCNNNNNNNGG 4 cut(s) 74, 448, 471, 517
AflIII ACRYGT 1 cut(s) 544
AjnI CCWGG 4 cut(s) 138, 238, 279, 447
AjuI GAANNNNNNNTTGG 4 cut(s) 653, 685, 833, 865
AluBI AGCT 6 cut(s) 17, 434, 556, 593, 630, 947
AluI AGCT 6 cut(s) 17, 434, 556, 593, 630, 947
Alw21I GWGCWC 1 cut(s) 605
Alw26I GTCTC 2 cut(s) 452, 713
AlwI GGATC 5 cut(s) 98, 327, 362, 367, 875
AlwNI CAGNNNCTG 1 cut(s) 145
AoxI GGCC 3 cut(s) 366, 450, 889
ApeKI GCWGC 5 cut(s) 8, 161, 380, 434, 590
ApoI RAATTY 2 cut(s) 488, 689
AspLEI GCGC 3 cut(s) 527, 684, 787
AspS9I GGNCC 1 cut(s) 890
AsuHPI GGTGA 5 cut(s) 216, 277, 295, 754, 877
AsuII TTCGAA 2 cut(s) 672, 905
AsuNHI GCTAGC 2 cut(s) 437, 556
BalI TGGCCA 1 cut(s) 452
BanII GRGCYC 1 cut(s) 477
BbsI GAAGAC 2 cut(s) 141, 914
Bbv12I GWGCWC 1 cut(s) 605
BbvI GCAGC 4 cut(s) 148, 367, 421, 602
BccI CCATC 3 cut(s) 49, 515, 980
BcgI CGANNNNNNTGC 2 cut(s) 359, 393
BciT130I CCWGG 4 cut(s) 140, 240, 281, 449
BciVI GTATCC 2 cut(s) 906, 978
BclI TGATCA 1 cut(s) 147
BcoDI GTCTC 2 cut(s) 452, 713
BfaI CTAG 2 cut(s) 438, 557
BfmI CTRYAG 5 cut(s) 12, 84, 231, 381, 594
BfoI RGCGCY 2 cut(s) 528, 788
BfuI GTATCC 2 cut(s) 906, 978
BisI GCNGC 6 cut(s) 6, 9, 162, 381, 435, 591
BlsI GCNGC 6 cut(s) 7, 10, 163, 382, 436, 592
Bme1390I CCNGG 4 cut(s) 140, 240, 281, 449
BmgT120I GGNCC 1 cut(s) 890
BmrFI CCNGG 4 cut(s) 140, 240, 281, 449
BmrI ACTGGG 1 cut(s) 170
BmsI GCATC 2 cut(s) 173, 183
BmtI GCTAGC 2 cut(s) 441, 560
BmuI ACTGGG 1 cut(s) 170
BpiI GAAGAC 2 cut(s) 141, 914
BpmI CTGGAG 1 cut(s) 925
Bpu14I TTCGAA 2 cut(s) 672, 905
BpuEI CTTGAG 1 cut(s) 389
BsaJI CCNNGG 3 cut(s) 239, 447, 660
BsaWI WCCGGW 2 cut(s) 818, 950
Bsc4I CCNNNNNNNGG 4 cut(s) 74, 448, 471, 517
Bse118I RCCGGY 2 cut(s) 790, 871
Bse1I ACTGG 3 cut(s) 176, 779, 892
BseBI CCWGG 4 cut(s) 140, 240, 281, 449
BseDI CCNNGG 3 cut(s) 239, 447, 660
BseGI GGATG 4 cut(s) 198, 310, 507, 972
BseLI CCNNNNNNNGG 4 cut(s) 74, 448, 471, 517
BseMII CTCAG 1 cut(s) 410
BseNI ACTGG 3 cut(s) 176, 779, 892
BseXI GCAGC 4 cut(s) 148, 367, 421, 602
BseYI CCCAGC 1 cut(s) 527
Bsh1236I CGCG 2 cut(s) 682, 684
BshFI GGCC 3 cut(s) 368, 452, 891
BsiHKAI GWGCWC 1 cut(s) 605
BsiSI CCGG 4 cut(s) 791, 819, 872, 951
BslI CCNNNNNNNGG 4 cut(s) 74, 448, 471, 517
BsmAI GTCTC 2 cut(s) 452, 713
BsnI GGCC 3 cut(s) 368, 452, 891
Bsp119I TTCGAA 2 cut(s) 672, 905
Bsp1286I GDGCHC 2 cut(s) 477, 605
Bsp143I GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 880
BspACI CCGC 4 cut(s) 5, 680, 740, 753
BspANI GGCC 3 cut(s) 368, 452, 891
BspCNI CTCAG 1 cut(s) 411
BspFNI CGCG 2 cut(s) 682, 684
BspMAI CTGCAG 2 cut(s) 88, 385
BspOI GCTAGC 2 cut(s) 441, 560
BspPI GGATC 5 cut(s) 98, 327, 362, 367, 875
BspT104I TTCGAA 2 cut(s) 672, 905
BsrFI RCCGGY 2 cut(s) 790, 871
BsrI ACTGG 3 cut(s) 176, 779, 892
BssAI RCCGGY 2 cut(s) 790, 871
BssECI CCNNGG 3 cut(s) 239, 447, 660
BssMI GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 880
BssT1I CCWWGG 1 cut(s) 660
Bst2UI CCWGG 4 cut(s) 140, 240, 281, 449
Bst4CI ACNGT 3 cut(s) 235, 826, 913
Bst6I CTCTTC 1 cut(s) 226
BstBI TTCGAA 2 cut(s) 672, 905
BstC8I GCNNGC 3 cut(s) 439, 558, 601
BstDEI CTNAG 2 cut(s) 342, 419
BstF5I GGATG 4 cut(s) 198, 310, 507, 972
BstFNI CGCG 2 cut(s) 682, 684
BstH2I RGCGCY 2 cut(s) 528, 788
BstHHI GCGC 3 cut(s) 527, 684, 787
BstKTI GATC 7 cut(s) 106, 150, 322, 357, 362, 619, 883
BstMAI GTCTC 2 cut(s) 452, 713
BstMBI GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 880
BstMWI GCNNNNNNNGC 6 cut(s) 14, 562, 596, 644, 737, 791
BstNI CCWGG 4 cut(s) 140, 240, 281, 449
BstNSI RCATGY 2 cut(s) 548, 569
BstSCI CCNGG 4 cut(s) 138, 238, 279, 447
BstSFI CTRYAG 5 cut(s) 12, 84, 231, 381, 594
BstUI CGCG 2 cut(s) 682, 684
BstV1I GCAGC 4 cut(s) 148, 367, 421, 602
BstV2I GAAGAC 2 cut(s) 141, 914
BstX2I RGATCY 2 cut(s) 319, 359
BstYI RGATCY 2 cut(s) 319, 359
BsuI GTATCC 2 cut(s) 906, 978
BsuRI GGCC 3 cut(s) 368, 452, 891
BtsCI GGATG 4 cut(s) 198, 310, 507, 972
BtsI GCAGTG 2 cut(s) 375, 738
BtsIMutI CAGTG 3 cut(s) 375, 738, 885
Cac8I GCNNGC 3 cut(s) 439, 558, 601
CaiI CAGNNNCTG 1 cut(s) 145
CfoI GCGC 3 cut(s) 527, 684, 787
Cfr10I RCCGGY 2 cut(s) 790, 871
Cfr13I GGNCC 1 cut(s) 890
CsiI ACCWGGT 1 cut(s) 138
Csp6I GTAC 4 cut(s) 64, 73, 373, 773
CviAII CATG 3 cut(s) 545, 566, 978
CviQI GTAC 4 cut(s) 64, 73, 373, 773
DdeI CTNAG 2 cut(s) 342, 419
DpnI GATC 7 cut(s) 105, 149, 321, 356, 361, 618, 882
DpnII GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 880
EaeI YGGCCR 2 cut(s) 366, 450
Eam1104I CTCTTC 1 cut(s) 226
EarI CTCTTC 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 660
Eco24I GRGCYC 1 cut(s) 477
Eco57I CTGAAG 1 cut(s) 860
EcoRI GAATTC 1 cut(s) 488
EcoRII CCWGG 4 cut(s) 138, 238, 279, 447
EcoT14I CCWWGG 1 cut(s) 660
EcoT38I GRGCYC 1 cut(s) 477
ErhI CCWWGG 1 cut(s) 660
FaeI CATG 3 cut(s) 548, 569, 981
FalI AAGNNNNNCTT 4 cut(s) 653, 685, 833, 865
FatI CATG 3 cut(s) 544, 565, 977
FauI CCCGC 1 cut(s) 746
FbaI TGATCA 1 cut(s) 147
Fnu4HI GCNGC 6 cut(s) 6, 9, 162, 381, 435, 591
FokI GGATG 4 cut(s) 205, 317, 494, 959
FriOI GRGCYC 1 cut(s) 477
Fsp4HI GCNGC 6 cut(s) 6, 9, 162, 381, 435, 591
FspBI CTAG 2 cut(s) 438, 557
GlaI GCGC 3 cut(s) 526, 683, 786
GluI GCNGC 6 cut(s) 6, 9, 162, 381, 435, 591
GsaI CCCAGC 1 cut(s) 531
GsuI CTGGAG 1 cut(s) 925
HaeII RGCGCY 2 cut(s) 528, 788
HaeIII GGCC 3 cut(s) 368, 452, 891
HapII CCGG 4 cut(s) 791, 819, 872, 951
HhaI GCGC 3 cut(s) 527, 684, 787
Hin1II CATG 3 cut(s) 548, 569, 981
Hin6I GCGC 3 cut(s) 525, 682, 785
HinP1I GCGC 3 cut(s) 525, 682, 785
HindIII AAGCTT 1 cut(s) 628
HinfI GANTC 3 cut(s) 26, 218, 534
HpaII CCGG 4 cut(s) 791, 819, 872, 951
HphI GGTGA 5 cut(s) 216, 277, 295, 754, 877
Hpy166II GTNNAC 3 cut(s) 49, 64, 375
Hpy188I TCNGA 4 cut(s) 108, 359, 420, 539
Hpy188III TCNNGA 3 cut(s) 122, 932, 942
Hpy8I GTNNAC 3 cut(s) 49, 64, 375
HpyAV CCTTC 7 cut(s) 452, 464, 730, 739, 743, 751, 856
HpyCH4III ACNGT 3 cut(s) 235, 826, 913
HpyCH4IV ACGT 1 cut(s) 775
HpyCH4V TGCA 4 cut(s) 86, 164, 383, 710
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 562, 596, 644, 737, 791
HpyF3I CTNAG 2 cut(s) 342, 419
HpySE526I ACGT 1 cut(s) 775
Hsp92II CATG 3 cut(s) 548, 569, 981
HspAI GCGC 3 cut(s) 525, 682, 785
Ksp22I TGATCA 1 cut(s) 147
Kzo9I GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 880
Lsp1109I GCAGC 4 cut(s) 148, 367, 421, 602
LweI GCATC 2 cut(s) 173, 183
MabI ACCWGGT 1 cut(s) 138
MaeI CTAG 2 cut(s) 438, 557
MaeII ACGT 1 cut(s) 775
MaeIII GTNAC 1 cut(s) 283
MalI GATC 7 cut(s) 105, 149, 321, 356, 361, 618, 882
MboI GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 880
MboII GAAGA 5 cut(s) 146, 213, 398, 686, 919
MfeI CAATTG 1 cut(s) 768
MflI RGATCY 2 cut(s) 319, 359
MhlI GDGCHC 2 cut(s) 477, 605
MlsI TGGCCA 1 cut(s) 452
MluCI AATT 3 cut(s) 488, 689, 768
MluNI TGGCCA 1 cut(s) 452
MlyI GAGTC 2 cut(s) 212, 543
MmeI TCCRAC 1 cut(s) 517
MnlI CCTC 3 cut(s) 345, 515, 949
Mox20I TGGCCA 1 cut(s) 452
MscI TGGCCA 1 cut(s) 452
MseI TTAA 1 cut(s) 294
Msp20I TGGCCA 1 cut(s) 452
MspI CCGG 4 cut(s) 791, 819, 872, 951
MspR9I CCNGG 4 cut(s) 140, 240, 281, 449
MunI CAATTG 1 cut(s) 768
MvaI CCWGG 4 cut(s) 140, 240, 281, 449
MvnI CGCG 2 cut(s) 682, 684
MwoI GCNNNNNNNGC 6 cut(s) 14, 562, 596, 644, 737, 791
NdeII GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 880
NheI GCTAGC 2 cut(s) 437, 556
NlaIII CATG 3 cut(s) 548, 569, 981
NmeAIII GCCGAG 1 cut(s) 394
NmuCI GTSAC 1 cut(s) 283
NspI RCATGY 2 cut(s) 548, 569
NspV TTCGAA 2 cut(s) 672, 905
PciI ACATGT 1 cut(s) 544
PfeI GAWTC 1 cut(s) 26
PkrI GCNGC 6 cut(s) 7, 10, 163, 382, 436, 592
PleI GAGTC 2 cut(s) 212, 542
PpsI GAGTC 2 cut(s) 212, 542
PscI ACATGT 1 cut(s) 544
PsiI TTATAA 1 cut(s) 705
Psp6I CCWGG 4 cut(s) 138, 238, 279, 447
PspFI CCCAGC 1 cut(s) 527
PspGI CCWGG 4 cut(s) 138, 238, 279, 447
PspPI GGNCC 1 cut(s) 890
PstI CTGCAG 2 cut(s) 88, 385
PstNI CAGNNNCTG 1 cut(s) 145
PsuI RGATCY 2 cut(s) 319, 359
RsaI GTAC 4 cut(s) 65, 74, 374, 774
RsaNI GTAC 4 cut(s) 64, 73, 373, 773
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 6 cut(s) 6, 9, 162, 381, 435, 591
Sau3AI GATC 7 cut(s) 103, 147, 319, 354, 359, 616, 880
Sau96I GGNCC 1 cut(s) 890
SchI GAGTC 2 cut(s) 212, 543
ScrFI CCNGG 4 cut(s) 140, 240, 281, 449
SduI GDGCHC 2 cut(s) 477, 605
SexAI ACCWGGT 1 cut(s) 138
SfaNI GCATC 2 cut(s) 173, 183
SfcI CTRYAG 5 cut(s) 12, 84, 231, 381, 594
SfuI TTCGAA 2 cut(s) 672, 905
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
Sse9I AATT 3 cut(s) 488, 689, 768
SsiI CCGC 4 cut(s) 5, 680, 740, 753
SspMI CTAG 2 cut(s) 438, 557
StyD4I CCNGG 4 cut(s) 138, 238, 279, 447
StyI CCWWGG 1 cut(s) 660
TaaI ACNGT 3 cut(s) 235, 826, 913
TaiI ACGT 1 cut(s) 778
TaqI TCGA 5 cut(s) 102, 180, 477, 672, 905
TasI AATT 3 cut(s) 488, 689, 768
TatI WGTACW 1 cut(s) 372
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 26
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 3 cut(s) 382, 738, 892
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 5 cut(s) 8, 161, 380, 434, 590
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 2 cut(s) 321, 483
TspGWI ACGGA 1 cut(s) 90
TspRI CASTG 3 cut(s) 382, 738, 892
XapI RAATTY 2 cut(s) 488, 689
XceI RCATGY 2 cut(s) 548, 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XspI CTAG 2 cut(s) 438, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.